Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006374

Physical Location & Seq

Genomic Coordinates Reverse (-)

13752263 .. 13756893

Gene Structure 4631 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G32400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2766 bp

ATGGGACTGGGCATTGACCCATCTGTCGCTATTACGGCACTGATTGTGGTCATTCTGGTGGTTCCAATGGATTGCCAAAGACCTCAACTGGTGAACATCGGTGCTGTTTTTGCTTTTGATTCGGTTATCGGAAGAGCTGCAAAAGTAGCTCTAGAGGCAGCTGTTTCCGATGTGAACAATGATAAAAGCTTTCTCAAAGAAACGGAGCTACGGCTATTAATGGAGGATTCTGCCTGCAATGTCTTTCGTGGGTCCTTTGGAGCTTTTGAATTGCTTGAGAAAGAAGTGGTGGCTATGATCGGTCCAATTTCATCCTCTGTTGCTCATACAATTTCCGATATTGCAAAAGGGCTCCACTTTCCTCTTGTCTCATTTGCAGCAACTGATCCAACTCTCTCTGCCCTCCAATTTCCCTTCTTTCTTCGGACTACACCTAATGATGCCCACCAAATGTCTGCCCTTGTGGATCTTATCAATTTTTATGGATGGAAAGAAGTGATCTCAGTTTACTCGGATGATGAGCTCGGAAGAAATGGAGTTTCTGCTCTAGATGATGAACTGTACAAGAAAAGATCCAGAATTTCCTATAAAGTGCCGCTCTCAGTTCATTCTGATGAAAAATTCCTTACTAATGCTTTGAACAAATCCAAGTCCATCGGTCCTCGAGTTTATATTCTTCATTTTGGTCCGGACCCATTGCTCAGAATTTTTGATATAGCGCAAAAGCTGCAGATGATGACCCATGAATACGTATGGCTCGCCACAGACTGGCTCTCTGTTACCTTAGATTCTTTGAGTGACAAAGGTACTCTAAAACGCCTTGAAGGAGTAGTGGGGCTTCGTCAACATATCCCAGAATCCGTAAAGATGGAACATTTCACGCATAAACTGCAGAGCAACAGATCAATGAATGCCTACGCATTACATGCCTATGATACAGTGTGGATGATTGCGCATGGCATCGAGGAATTGCTGAATGAAGGAATCAACATAACGTTTTCTTACTCCGAAAAGTTACTTCATGCACGGGGAACCAAACTGCACTTGGAGAAAATCAAATTTTTCAACAGTGGGGAGTTACTACTTGAGAAACTTCTGAAAGTAAACTTTACTGGTATAGCCGGTCAAGTTCAGTTTGGTTCTGGCCGAAACGTTATCGGCTGTGACTACGAAATTATCAATGTAAACAAAACCGATGTTCATACTGTCGGTTTCTGGTCGAAAAATGGAGGCTTTTCGGTTGTAGCCCCAAAAACCCGTCATTCACAGAAGAAGACTAGCTTTGTTTCTGATGAAAAACTTGGAGATATAACCTGGCCTGGTGGTGGCCGTGAAAAGCCACGTGGTTGGGTCATTGCAGATTCTGCAGATCCATTGAAGATTGTTGTCCCGAGAAGAGTGAGTTTTGTCGAGTTTGTGACTGAAGAAAAGAACAGTAGCCATCGGATCCAAGGATTTTGCATCGATGTCTTCATTGAAGCATTAAAGTTCGTCCCTTACAGTGTTCCTTACATATTTGAGCCTTTCGGGAATGGTCATTCAAGTCCTAATTACAACCACCTTATTCAAATGGTCACGGATGGTGTATATGATGCTGCTGTTGGGGATATTGCAATAGTCCCAAGCCGGTCCAAGTTAGTGGATTTCTCGCAGCCATATGCTTCCACAGGCCTTGTAGTGGTGATTCCTGCTAATGATGACAATGCGACTTGGATCTTTCTGAGACCCTTCACCAGCCGGTTATGGTGTGTTGTTCTAGTTTCATTCCTGGTTATTGCCGTAGTCATCTGGATCCTCGAACATCGCATCAATGAAGATTTCAGAGGGCCACCCCGAAGACAACTCAGCACAATGCTCTTGTTCAGCTTCTCAACTCTTTTCAAGAGAAACCAGGAAGATACAATAAGTAATCTAGCAAGACTAGTGATGATTGTATGGCTCTTCCTATTGATGGTTCTAACCGCGAGCTACACAGCGAACCTCACCTCAATCCTCACAGTTCAACAGCTTCCCTCTGCTATTACCGGTATTGATAGCTTGCGGGCAAGTGAGGTACCTATCGGGTACCAGGCTGGGACGTTCACTTTAGAGTATTTAACCTACAGTCTTGGCATGGCTCGGTCTAGACTGGTCCCACTTGACTCGACCGAGGAGTATGAAAAGGCTCTAAAGCTGGGGCCAACCAATTGGGGAGGTGTTGCTGCCATTGTCGACGAACTCCCTTACATCGAACTGTTTCTAGCAGAACGAACAGGCTTCAAGATTGTTGGAGAACCCTTTATGCACCGTGGTTGGGGATTTGCGTTTAAGAGAGATTCTCCATTGGCTATAGACATGTCAACAGCTATCTTGAAACTCTCTGAGACGAGGAAATTGCAAGAAATTCGAAAGAAATGGTTATGCAAGACGAATTGCGCAGGGAAATCAAATTGGAACCCAGAGCCAAACCAGCTTCATCTCAAAAGCTTCAAAGGGCTGTACCTTGTCTGCATTGCAATCACAGTCTCTGCGTTTTTGGTGTTTGTTCTCAGGATGATACGCCAGTTCGTGCGGTACAGACGGATGGAGAGAACATCCTCGATGCCACGCGCTTCTTGGTCAGCTTCTCCTACATTGCGGTTGAGAGAATTAGTGTTTGATTTTGTGGAGTTTGTGGATGAGAAAGAAGAAGCTATTAAGAGAATGTTCAGAAGAAGCGATGACTCTAATAACAACCCATCTCATGTAGGGGAAGTCCAAGCTGATACTGAGGTACCACGAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009506 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0030054 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055044 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

921

Amino Acids

103.51

Weight (kDa)

7.66

Isoelectric Point (pI)

36.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	42 - 323	8.3e-12	Periplasmic binding protein
ANF_receptor	PF01094	48 - 397	1e-92	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	474 - 564	1.2e-09	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	517 - 800	6.4e-11	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	579 - 831	1.7e-36	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006374)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32400
fragaria_vesca	FvH4_6g43290 FvH4_6g43290 FvH4_6g43290 FvH4_6g43290
malus_domestica	MD09G1070000.v1.1 MD09G1108300.v1.1 MD17G1096200.v1.1
prunus_persica	Prupe.3G217800_v2.0.a1 Prupe.3G217800_v2.0.a1 Prupe.3G217800_v2.0.a1
pyrus_communis	pycom09g03020 pycom09g03080 pycom17g09230
rosa_chinensis	RchiOBHm_Chr2g0159561
rosa_laevigata	RLG00000021166
rosa_multiflora	Rmu_sc0012414.1_g000014 Rmu_sc0012414.1_g000015
rosa_roxburghii	Rroxscaffold_2G00090400
rosa_rugosa	Rorug02G0476900 Rorug02G0476900
rosa_samantha	Rh2AG543800 Rh2AG544100 Rh2BG556900 Rh2CG527100 Rh2DG565900
rosa_wichuraiana	Rw2G044980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	23
Acc16I	TGCGCA	2 cut(s)	954, 2416
Acc65I	GGTACC	3 cut(s)	2053, 2064, 2752
AccB1I	GGYRCC	3 cut(s)	2053, 2064, 2752
AccB7I	CCANNNNNTGG	1 cut(s)	768
AccBSI	CCGCTC	1 cut(s)	598
AccI	GTMKAC	1 cut(s)	2211
AccII	CGCG	2 cut(s)	1964, 2589
AccIII	TCCGGA	1 cut(s)	688
AciI	CCGC	5 cut(s)	596, 1962, 2041, 2551, 2617
AclI	AACGTT	2 cut(s)	995, 1152
AclWI	GGATC	9 cut(s)	380, 474, 567, 1364, 1441, 1454, 1721, 1786, 1799
AcoI	YGGCCR	2 cut(s)	1144, 1327
AcsI	RAATTY	5 cut(s)	579, 620, 705, 1058, 2382
AcuI	CTGAAG	1 cut(s)	1443
AcvI	CACGTG	1 cut(s)	1343
AfaI	GTAC	7 cut(s)	563, 808, 2055, 2066, 2480, 2555, 2754
AfiI	CCNNNNNNNGG	5 cut(s)	768, 1325, 1678, 1951, 2293
AflIII	ACRYGT	1 cut(s)	2334
AgeI	ACCGGT	1 cut(s)	2024
AhlI	ACTAGT	1 cut(s)	1921
AjnI	CCWGG	5 cut(s)	1313, 1318, 1767, 1890, 2067
Alw21I	GWGCWC	1 cut(s)	525
Alw26I	GTCTC	4 cut(s)	373, 1717, 2357, 2509
AlwI	GGATC	9 cut(s)	380, 474, 567, 1364, 1441, 1454, 1721, 1786, 1799
AlwNI	CAGNNNCTG	3 cut(s)	383, 1364, 2507
Ama87I	CYCGRG	2 cut(s)	663, 1390
Aor13HI	TCCGGA	1 cut(s)	688
AoxI	GGCC	6 cut(s)	1144, 1316, 1327, 1669, 1826, 2177
ApeKI	GCWGC	7 cut(s)	137, 158, 377, 727, 1595, 1651, 2201
ApoI	RAATTY	5 cut(s)	579, 620, 705, 1058, 2382
AseI	ATTAAT	1 cut(s)	218
AsiGI	ACCGGT	1 cut(s)	2024
Asp700I	GAANNNNTTC	1 cut(s)	2235
Asp718I	GGTACC	3 cut(s)	2053, 2064, 2752
AspLEI	GCGC	4 cut(s)	721, 955, 2417, 2591
AspS9I	GGNCC	9 cut(s)	252, 302, 659, 686, 691, 1629, 1826, 2131, 2177
AsuHPI	GGTGA	4 cut(s)	103, 1693, 1723, 1975
AsuII	TTCGAA	1 cut(s)	2386
AvaI	CYCGRG	2 cut(s)	663, 1390
AvaII	GGWCC	7 cut(s)	252, 302, 659, 686, 691, 1629, 2131
BaeI	ACNNNNGTAYC	4 cut(s)	2528, 2561, 2736, 2736
BamHI	GGATCC	2 cut(s)	1446, 1791
BanI	GGYRCC	3 cut(s)	2053, 2064, 2752
BanII	GRGCYC	2 cut(s)	354, 525
BarI	GAAGNNNNNNTAC	2 cut(s)	822, 854
BbrPI	CACGTG	1 cut(s)	1343
BbsI	GAAGAC	3 cut(s)	1280, 1462, 1843
Bbv12I	GWGCWC	1 cut(s)	525
BbvI	GCAGC	7 cut(s)	124, 170, 389, 714, 1582, 1663, 2188
BccI	CCATC	9 cut(s)	28, 480, 662, 862, 1449, 1574, 1945, 2557, 2725
BceAI	ACGGC	4 cut(s)	51, 227, 1314, 1763
BcgI	CGANNNNNNTGC	4 cut(s)	2356, 2366, 2390, 2400
BciT130I	CCWGG	5 cut(s)	1315, 1320, 1769, 1892, 2069
BcoDI	GTCTC	4 cut(s)	373, 1717, 2357, 2509
BcuI	ACTAGT	1 cut(s)	1921
BfaI	CTAG	8 cut(s)	152, 548, 1278, 1757, 1913, 1922, 2124, 2240
BfmI	CTRYAG	5 cut(s)	728, 890, 1365, 2101, 2328
BisI	GCNGC	8 cut(s)	138, 159, 378, 596, 728, 1596, 1652, 2202
BlsI	GCNGC	8 cut(s)	139, 160, 379, 597, 729, 1597, 1653, 2203
Bme1390I	CCNGG	5 cut(s)	1315, 1320, 1769, 1892, 2069
Bme18I	GGWCC	7 cut(s)	252, 302, 659, 686, 691, 1629, 2131
BmeT110I	CYCGRG	2 cut(s)	663, 1390
BmgT120I	GGNCC	9 cut(s)	252, 302, 659, 686, 691, 1629, 1826, 2131, 2177
BmrFI	CCNGG	5 cut(s)	1315, 1320, 1769, 1892, 2069
BmrI	ACTGGG	1 cut(s)	17
BmsI	GCATC	6 cut(s)	430, 969, 1470, 1582, 1815, 2571
BmuI	ACTGGG	1 cut(s)	17
BpiI	GAAGAC	3 cut(s)	1280, 1462, 1843
BplI	GAGNNNNNCTC	2 cut(s)	2302, 2334
Bpu14I	TTCGAA	1 cut(s)	2386
BpuEI	CTTGAG	2 cut(s)	296, 1106
Bsa29I	ATCGAT	1 cut(s)	1464
BsaAI	YACGTR	2 cut(s)	751, 1343
BsaI	GGTCTC	1 cut(s)	1717
BsaJI	CCNNGG	3 cut(s)	1450, 2148, 2287
BsaWI	WCCGGW	2 cut(s)	688, 2024
Bsc4I	CCNNNNNNNGG	5 cut(s)	768, 1325, 1678, 1951, 2293
Bse118I	RCCGGY	4 cut(s)	1121, 1626, 1737, 2024
Bse1I	ACTGG	6 cut(s)	12, 93, 773, 1117, 2133, 2542
Bse3DI	GCAATG	5 cut(s)	244, 695, 1353, 2490, 2612
BseAI	TCCGGA	1 cut(s)	688
BseBI	CCWGG	5 cut(s)	1315, 1320, 1769, 1892, 2069
BseCI	ATCGAT	1 cut(s)	1464
BseDI	CCNNGG	3 cut(s)	1450, 2148, 2287
BseGI	GGATG	9 cut(s)	311, 491, 520, 951, 1585, 2538, 2568, 2573, 2662
BseLI	CCNNNNNNNGG	5 cut(s)	768, 1325, 1678, 1951, 2293
BseMI	GCAATG	5 cut(s)	244, 695, 1353, 2490, 2612
BseMII	CTCAG	8 cut(s)	516, 615, 715, 1712, 1858, 2352, 2542, 2739
BseNI	ACTGG	6 cut(s)	12, 93, 773, 1117, 2133, 2542
BseRI	GAGGAG	1 cut(s)	2165
BseXI	GCAGC	7 cut(s)	124, 170, 389, 714, 1582, 1663, 2188
BseYI	CCCAGC	2 cut(s)	2072, 2173
BsgI	GTGCAG	1 cut(s)	1025
Bsh1236I	CGCG	2 cut(s)	1964, 2589
Bsh1285I	CGRYCG	1 cut(s)	2148
BshFI	GGCC	6 cut(s)	1146, 1318, 1329, 1671, 1828, 2179
BshNI	GGYRCC	3 cut(s)	2053, 2064, 2752
BshTI	ACCGGT	1 cut(s)	2024
BshVI	ATCGAT	1 cut(s)	1464
BsiEI	CGRYCG	1 cut(s)	2148
BsiHKAI	GWGCWC	1 cut(s)	525
BsiHKCI	CYCGRG	2 cut(s)	663, 1390
BsiSI	CCGG	5 cut(s)	689, 1122, 1627, 1738, 2025
BslFI	GGGAC	6 cut(s)	18, 1373, 1478, 1604, 2089, 2117
BslI	CCNNNNNNNGG	5 cut(s)	768, 1325, 1678, 1951, 2293
BsmAI	GTCTC	4 cut(s)	373, 1717, 2357, 2509
BsmBI	CGTCTC	1 cut(s)	2357
BsmFI	GGGAC	6 cut(s)	18, 1373, 1478, 1604, 2089, 2117
BsmI	GAATGC	1 cut(s)	916
BsnI	GGCC	6 cut(s)	1146, 1318, 1329, 1671, 1828, 2179
Bso31I	GGTCTC	1 cut(s)	1717
BsoBI	CYCGRG	2 cut(s)	663, 1390
Bsp119I	TTCGAA	1 cut(s)	2386
Bsp1286I	GDGCHC	2 cut(s)	354, 525
Bsp13I	TCCGGA	1 cut(s)	688
Bsp1407I	TGTACA	1 cut(s)	561
BspACI	CCGC	5 cut(s)	596, 1962, 2041, 2551, 2617
BspANI	GGCC	6 cut(s)	1146, 1318, 1329, 1671, 1828, 2179
BspCNI	CTCAG	8 cut(s)	515, 614, 714, 1713, 1857, 2353, 2541, 2740
BspDI	ATCGAT	1 cut(s)	1464
BspEI	TCCGGA	1 cut(s)	688
BspFNI	CGCG	2 cut(s)	1964, 2589
BspMAI	CTGCAG	3 cut(s)	732, 894, 1369
BspPI	GGATC	9 cut(s)	380, 474, 567, 1364, 1441, 1454, 1721, 1786, 1799
BspQI	GCTCTTC	2 cut(s)	127, 1946
BspT104I	TTCGAA	1 cut(s)	2386
BspT107I	GGYRCC	3 cut(s)	2053, 2064, 2752
BspTNI	GGTCTC	1 cut(s)	1717
BsrBI	CCGCTC	1 cut(s)	598
BsrDI	GCAATG	5 cut(s)	244, 695, 1353, 2490, 2612
BsrFI	RCCGGY	4 cut(s)	1121, 1626, 1737, 2024
BsrGI	TGTACA	1 cut(s)	561
BsrI	ACTGG	6 cut(s)	12, 93, 773, 1117, 2133, 2542
BssAI	RCCGGY	4 cut(s)	1121, 1626, 1737, 2024
BssECI	CCNNGG	3 cut(s)	1450, 2148, 2287
BssT1I	CCWWGG	1 cut(s)	1450
Bst2UI	CCWGG	5 cut(s)	1315, 1320, 1769, 1892, 2069
Bst6I	CTCTTC	3 cut(s)	127, 1390, 1946
BstAPI	GCANNNNNTGC	4 cut(s)	727, 889, 926, 1364
BstAUI	TGTACA	1 cut(s)	561
BstBAI	YACGTR	2 cut(s)	751, 1343
BstBI	TTCGAA	1 cut(s)	2386
BstC8I	GCNNGC	5 cut(s)	235, 759, 1966, 2039, 2043
BstDEI	CTNAG	9 cut(s)	502, 601, 701, 784, 1721, 1844, 2361, 2528, 2748
BstDSI	CCRYGG	1 cut(s)	2287
BstF5I	GGATG	9 cut(s)	311, 491, 520, 951, 1585, 2538, 2568, 2573, 2662
BstFNI	CGCG	2 cut(s)	1964, 2589
BstHHI	GCGC	4 cut(s)	721, 955, 2417, 2591
BstMAI	GTCTC	4 cut(s)	373, 1717, 2357, 2509
BstMCI	CGRYCG	1 cut(s)	2148
BstMWI	GCNNNNNNNGC	9 cut(s)	35, 110, 146, 155, 727, 889, 926, 1364, 2449
BstNI	CCWGG	5 cut(s)	1315, 1320, 1769, 1892, 2069
BstNSI	RCATGY	2 cut(s)	929, 2338
BstSCI	CCNGG	5 cut(s)	1313, 1318, 1767, 1890, 2067
BstSFI	CTRYAG	5 cut(s)	728, 890, 1365, 2101, 2328
BstSNI	TACGTA	1 cut(s)	751
BstUI	CGCG	2 cut(s)	1964, 2589
BstV1I	GCAGC	7 cut(s)	124, 170, 389, 714, 1582, 1663, 2188
BstV2I	GAAGAC	3 cut(s)	1280, 1462, 1843
BstX2I	RGATCY	6 cut(s)	466, 572, 1369, 1446, 1713, 1791
BstXI	CCANNNNNNTGG	3 cut(s)	1347, 1639, 2187
BstYI	RGATCY	6 cut(s)	466, 572, 1369, 1446, 1713, 1791
Bsu15I	ATCGAT	1 cut(s)	1464
BsuRI	GGCC	6 cut(s)	1146, 1318, 1329, 1671, 1828, 2179
BsuTUI	ATCGAT	1 cut(s)	1464
BtgI	CCRYGG	1 cut(s)	2287
BtgZI	GCGATG	2 cut(s)	1787, 2712
BtsCI	GGATG	9 cut(s)	311, 491, 520, 951, 1585, 2538, 2568, 2573, 2662
BtsIMutI	CAGTG	4 cut(s)	38, 945, 1075, 1507
Cac8I	GCNNGC	5 cut(s)	235, 759, 1966, 2039, 2043
CaiI	CAGNNNCTG	3 cut(s)	383, 1364, 2507
CfoI	GCGC	4 cut(s)	721, 955, 2417, 2591
Cfr10I	RCCGGY	4 cut(s)	1121, 1626, 1737, 2024
Cfr13I	GGNCC	9 cut(s)	252, 302, 659, 686, 691, 1629, 1826, 2131, 2177
ClaI	ATCGAT	1 cut(s)	1464
Csp6I	GTAC	7 cut(s)	562, 807, 2054, 2065, 2479, 2554, 2753
CspAI	ACCGGT	1 cut(s)	2024
CspCI	CAANNNNNGTGG	2 cut(s)	1654, 1689
CviAII	CATG	7 cut(s)	743, 926, 956, 1022, 2113, 2335, 2723
CviQI	GTAC	7 cut(s)	562, 807, 2054, 2065, 2479, 2554, 2753
DdeI	CTNAG	9 cut(s)	502, 601, 701, 784, 1721, 1844, 2361, 2528, 2748
DrdI	GACNNNNNNGTC	1 cut(s)	23
DseDI	GACNNNNNNGTC	1 cut(s)	23
EaeI	YGGCCR	2 cut(s)	1144, 1327
Eam1104I	CTCTTC	3 cut(s)	127, 1390, 1946
EarI	CTCTTC	3 cut(s)	127, 1390, 1946
Ecl136II	GAGCTC	1 cut(s)	523
Eco105I	TACGTA	1 cut(s)	751
Eco130I	CCWWGG	1 cut(s)	1450
Eco147I	AGGCCT	1 cut(s)	1671
Eco24I	GRGCYC	2 cut(s)	354, 525
Eco31I	GGTCTC	1 cut(s)	1717
Eco47I	GGWCC	7 cut(s)	252, 302, 659, 686, 691, 1629, 2131
Eco53kI	GAGCTC	1 cut(s)	523
Eco57I	CTGAAG	1 cut(s)	1443
Eco72I	CACGTG	1 cut(s)	1343
Eco88I	CYCGRG	2 cut(s)	663, 1390
EcoICRI	GAGCTC	1 cut(s)	523
EcoO109I	RGGNCCY	1 cut(s)	252
EcoRII	CCWGG	5 cut(s)	1313, 1318, 1767, 1890, 2067
EcoT14I	CCWWGG	1 cut(s)	1450
EcoT38I	GRGCYC	2 cut(s)	354, 525
ErhI	CCWWGG	1 cut(s)	1450
Esp3I	CGTCTC	1 cut(s)	2357
FaeI	CATG	7 cut(s)	746, 929, 959, 1025, 2116, 2338, 2726
FalI	AAGNNNNNCTT	2 cut(s)	1265, 1297
FaqI	GGGAC	6 cut(s)	18, 1373, 1478, 1604, 2089, 2117
FatI	CATG	7 cut(s)	742, 925, 955, 1021, 2112, 2334, 2722
FauI	CCCGC	1 cut(s)	2034
FauNDI	CATATG	1 cut(s)	1657
FblI	GTMKAC	1 cut(s)	2211
Fnu4HI	GCNGC	8 cut(s)	138, 159, 378, 596, 728, 1596, 1652, 2202
FokI	GGATG	9 cut(s)	298, 498, 527, 958, 1592, 2545, 2560, 2575, 2669
FriOI	GRGCYC	2 cut(s)	354, 525
Fsp4HI	GCNGC	8 cut(s)	138, 159, 378, 596, 728, 1596, 1652, 2202
FspBI	CTAG	8 cut(s)	152, 548, 1278, 1757, 1913, 1922, 2124, 2240
FspI	TGCGCA	2 cut(s)	954, 2416
GlaI	GCGC	4 cut(s)	720, 954, 2416, 2590
GluI	GCNGC	8 cut(s)	138, 159, 378, 596, 728, 1596, 1652, 2202
GsaI	CCCAGC	2 cut(s)	2076, 2177
HaeIII	GGCC	6 cut(s)	1146, 1318, 1329, 1671, 1828, 2179
HapII	CCGG	5 cut(s)	689, 1122, 1627, 1738, 2025
HhaI	GCGC	4 cut(s)	721, 955, 2417, 2591
Hin1II	CATG	7 cut(s)	746, 929, 959, 1025, 2116, 2338, 2726
Hin6I	GCGC	4 cut(s)	719, 953, 2415, 2589
HinP1I	GCGC	4 cut(s)	719, 953, 2415, 2589
HincII	GTYRAC	3 cut(s)	845, 2212, 2340
HindII	GTYRAC	3 cut(s)	845, 2212, 2340
HindIII	AAGCTT	2 cut(s)	187, 2464
HpaII	CCGG	5 cut(s)	689, 1122, 1627, 1738, 2025
HphI	GGTGA	4 cut(s)	103, 1693, 1723, 1975
Hpy166II	GTNNAC	9 cut(s)	94, 175, 508, 845, 1105, 1186, 2082, 2212, 2340
Hpy8I	GTNNAC	9 cut(s)	94, 175, 508, 845, 1105, 1186, 2082, 2212, 2340
Hpy99I	CGWCG	1 cut(s)	2216
HpyAV	CCTTC	4 cut(s)	424, 818, 974, 1738
HpyCH4IV	ACGT	5 cut(s)	750, 995, 1152, 1342, 2078
HpyF10VI	GCNNNNNNNGC	9 cut(s)	35, 110, 146, 155, 727, 889, 926, 1364, 2449
HpyF3I	CTNAG	9 cut(s)	502, 601, 701, 784, 1721, 1844, 2361, 2528, 2748
HpySE526I	ACGT	5 cut(s)	750, 995, 1152, 1342, 2078
Hsp92II	CATG	7 cut(s)	746, 929, 959, 1025, 2116, 2338, 2726
HspAI	GCGC	4 cut(s)	719, 953, 2415, 2589
Kpn2I	TCCGGA	1 cut(s)	688
KpnI	GGTACC	3 cut(s)	2057, 2068, 2756
LguI	GCTCTTC	2 cut(s)	127, 1946
LmnI	GCTCC	3 cut(s)	205, 260, 357
Lsp1109I	GCAGC	7 cut(s)	124, 170, 389, 714, 1582, 1663, 2188
LweI	GCATC	6 cut(s)	430, 969, 1470, 1582, 1815, 2571
MaeI	CTAG	8 cut(s)	152, 548, 1278, 1757, 1913, 1922, 2124, 2240
MaeII	ACGT	5 cut(s)	750, 995, 1152, 1342, 2078
MaeIII	GTNAC	7 cut(s)	778, 797, 1014, 1077, 1163, 1417, 1573
MbiI	CCGCTC	1 cut(s)	598
MfeI	CAATTG	1 cut(s)	2185
MflI	RGATCY	6 cut(s)	466, 572, 1369, 1446, 1713, 1791
MhlI	GDGCHC	2 cut(s)	354, 525
MlyI	GAGTC	2 cut(s)	2135, 2696
MmeI	TCCRAC	2 cut(s)	413, 2248
MroI	TCCGGA	1 cut(s)	688
MroXI	GAANNNNTTC	1 cut(s)	2235
MseI	TTAA	5 cut(s)	218, 1484, 2096, 2307, 2676
MslI	CAYNNNNRTG	3 cut(s)	56, 612, 930
MspA1I	CMGCKG	1 cut(s)	161
MspI	CCGG	5 cut(s)	689, 1122, 1627, 1738, 2025
MspR9I	CCNGG	5 cut(s)	1315, 1320, 1769, 1892, 2069
MunI	CAATTG	1 cut(s)	2185
Mva1269I	GAATGC	1 cut(s)	916
MvaI	CCWGG	5 cut(s)	1315, 1320, 1769, 1892, 2069
MvnI	CGCG	2 cut(s)	1964, 2589
MwoI	GCNNNNNNNGC	9 cut(s)	35, 110, 146, 155, 727, 889, 926, 1364, 2449
NdeI	CATATG	1 cut(s)	1657
NlaIII	CATG	7 cut(s)	746, 929, 959, 1025, 2116, 2338, 2726
NmuCI	GTSAC	4 cut(s)	797, 1163, 1417, 1573
NsbI	TGCGCA	2 cut(s)	954, 2416
NspI	RCATGY	2 cut(s)	929, 2338
NspV	TTCGAA	1 cut(s)	2386
PaeR7I	CTCGAG	1 cut(s)	663
PceI	AGGCCT	1 cut(s)	1671
PciI	ACATGT	1 cut(s)	2334
PciSI	GCTCTTC	2 cut(s)	127, 1946
PctI	GAATGC	1 cut(s)	916
PdmI	GAANNNNTTC	1 cut(s)	2235
PfeI	GAWTC	8 cut(s)	119, 227, 788, 857, 984, 1361, 1684, 2315
PflMI	CCANNNNNTGG	1 cut(s)	768
PinAI	ACCGGT	1 cut(s)	2024
PkrI	GCNGC	8 cut(s)	139, 160, 379, 597, 729, 1597, 1653, 2203
PleI	GAGTC	2 cut(s)	2135, 2696
PmaCI	CACGTG	1 cut(s)	1343
PmlI	CACGTG	1 cut(s)	1343
PpsI	GAGTC	2 cut(s)	2135, 2696
Ppu21I	YACGTR	2 cut(s)	751, 1343
PpuMI	RGGWCCY	1 cut(s)	252
PscI	ACATGT	1 cut(s)	2334
PshBI	ATTAAT	1 cut(s)	218
Psp124BI	GAGCTC	1 cut(s)	525
Psp1406I	AACGTT	2 cut(s)	995, 1152
Psp5II	RGGWCCY	1 cut(s)	252
Psp6I	CCWGG	5 cut(s)	1313, 1318, 1767, 1890, 2067
PspCI	CACGTG	1 cut(s)	1343
PspFI	CCCAGC	2 cut(s)	2072, 2173
PspGI	CCWGG	5 cut(s)	1313, 1318, 1767, 1890, 2067
PspPI	GGNCC	9 cut(s)	252, 302, 659, 686, 691, 1629, 1826, 2131, 2177
PspPPI	RGGWCCY	1 cut(s)	252
PspXI	VCTCGAGB	1 cut(s)	663
PstI	CTGCAG	3 cut(s)	732, 894, 1369
PstNI	CAGNNNCTG	3 cut(s)	383, 1364, 2507
PsuI	RGATCY	6 cut(s)	466, 572, 1369, 1446, 1713, 1791
PvuII	CAGCTG	1 cut(s)	161
RsaI	GTAC	7 cut(s)	563, 808, 2055, 2066, 2480, 2555, 2754
RsaNI	GTAC	7 cut(s)	562, 807, 2054, 2065, 2479, 2554, 2753
RseI	CAYNNNNRTG	3 cut(s)	56, 612, 930
SacI	GAGCTC	1 cut(s)	525
SalI	GTCGAC	1 cut(s)	2210
SapI	GCTCTTC	2 cut(s)	127, 1946
SaqAI	TTAA	5 cut(s)	218, 1484, 2096, 2307, 2676
SatI	GCNGC	8 cut(s)	138, 159, 378, 596, 728, 1596, 1652, 2202
Sau96I	GGNCC	9 cut(s)	252, 302, 659, 686, 691, 1629, 1826, 2131, 2177
SchI	GAGTC	2 cut(s)	2135, 2696
ScrFI	CCNGG	5 cut(s)	1315, 1320, 1769, 1892, 2069
SduI	GDGCHC	2 cut(s)	354, 525
SfaNI	GCATC	6 cut(s)	430, 969, 1470, 1582, 1815, 2571
SfcI	CTRYAG	5 cut(s)	728, 890, 1365, 2101, 2328
Sfr274I	CTCGAG	1 cut(s)	663
SfuI	TTCGAA	1 cut(s)	2386
SinI	GGWCC	7 cut(s)	252, 302, 659, 686, 691, 1629, 2131
SlaI	CTCGAG	1 cut(s)	663
SmiMI	CAYNNNNRTG	3 cut(s)	56, 612, 930
SmlI	CTYRAG	3 cut(s)	275, 663, 1085
SmoI	CTYRAG	3 cut(s)	275, 663, 1085
SnaBI	TACGTA	1 cut(s)	751
SpeI	ACTAGT	1 cut(s)	1921
SseBI	AGGCCT	1 cut(s)	1671
SsiI	CCGC	5 cut(s)	596, 1962, 2041, 2551, 2617
SspMI	CTAG	8 cut(s)	152, 548, 1278, 1757, 1913, 1922, 2124, 2240
SstI	GAGCTC	1 cut(s)	525
StuI	AGGCCT	1 cut(s)	1671
StyD4I	CCNGG	5 cut(s)	1313, 1318, 1767, 1890, 2067
StyI	CCWWGG	1 cut(s)	1450
TaiI	ACGT	5 cut(s)	753, 998, 1155, 1345, 2081
TaqII	GACCGA	4 cut(s)	290, 647, 2109, 2162
TatI	WGTACW	1 cut(s)	561
TauI	GCSGC	1 cut(s)	598
TfiI	GAWTC	8 cut(s)	119, 227, 788, 857, 984, 1361, 1684, 2315
Tru1I	TTAA	5 cut(s)	218, 1484, 2096, 2307, 2676
Tru9I	TTAA	5 cut(s)	218, 1484, 2096, 2307, 2676
TscAI	CASTG	4 cut(s)	45, 945, 1075, 1507
TseFI	GTSAC	4 cut(s)	797, 1163, 1417, 1573
TseI	GCWGC	7 cut(s)	137, 158, 377, 727, 1595, 1651, 2201
Tsp45I	GTSAC	4 cut(s)	797, 1163, 1417, 1573
TspGWI	ACGGA	4 cut(s)	218, 850, 1592, 2575
TspRI	CASTG	4 cut(s)	45, 945, 1075, 1507
Van91I	CCANNNNNTGG	1 cut(s)	768
VpaK11BI	GGWCC	7 cut(s)	252, 302, 659, 686, 691, 1629, 2131
VspI	ATTAAT	1 cut(s)	218
XapI	RAATTY	5 cut(s)	579, 620, 705, 1058, 2382
XbaI	TCTAGA	3 cut(s)	151, 547, 2123
XceI	RCATGY	2 cut(s)	929, 2338
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1042, 2592
XhoI	CTCGAG	1 cut(s)	663
XmiI	GTMKAC	1 cut(s)	2211
XmnI	GAANNNNTTC	1 cut(s)	2235
XspI	CTAG	8 cut(s)	152, 548, 1278, 1757, 1913, 1922, 2124, 2240

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.