Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0006374

Physical Location & Seq

Genomic Coordinates Reverse (-)

4802333 .. 4806994

Gene Structure 4662 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1070000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2739 bp

ATGAGGGGTGCTGTAGTTTTGCCACTGCAATTTCTGATATGGGTTTTCCTCACTAGCTGCTTTTACTGCCAGAAGCCTTCTGTGGTGAATATTGGAGCAATTTTTAGTTTTAACTCGGTTATTGGTAGAGTTGCAAAGACAGCCATGGAAGCTGCACTCTCTGATGTTAATGCAGACCCAAGAATTCTCAGTGGGACGGAGCTGAGGTTGCTTAAGGAGAATACAAATTCTAGTGTGTTCTTGGGATCCGTTGAAGCTTTTCAGGTGCTTGACAAAAGTATAGTAGCCATAATCGGACCACAGACCTCTTCAATGGCTCATATGATCTCTGAAATTGCTAATGGTCTCCAAGTACCTCTTATCTCATATGCTGCCACTGATCCTACTCTTTCTGCTCTCCAATTCCCATTCTTTCTCCGAACTATACAAAGCGATGCTCACCAAATGGCTGCTATGGCTGGTTTGATTGATTTTTACGGGTGGAAAGAGGTTATTGCTGTCTTTGTGGATGACGATTATGGGAGGAATGGAATATCTGCTTTACATGATGAACTTGACAAAACAATGTCAAGGATTTCTCATAACTTGGCTTTGCCTGTCCAATATAATTTAAATGACATTGCTGATTTGCTCAATAAATCCAGATTTCTTGGCCCTCGTGTCTATATTGTTCATGTTGATCCCGATCCTAGGTTGAGAATCTTTACTGTCGCCAAAGAACTTCATATGATGACAAGCAATTATGTGTGGTTTGCAACAGATTGGCTTTCTACTACAGTAGATTCATTCTCTCCAATGAACCGAACTTCACTCGCTGCCCTTCAAGGTGTAGTTACTTTACGTAAGCATATCCCAGAGTCCAGTCGAAAGCGTGCATTTATGTCTAGGTGGAAAAAAATGCAGCAGGATGGCTTAGCAAGTTCTGAGTTAAATGTCTATGGACTTTACGCTTATGACACAGTTTGGGCTGTTGCACATTCGATTGAAAATTTTATGAATGAAAACAGAAATATCTCATTCTCTGCCCTTGATAAATTACTTGACATGAGACCATCTCAAATTCAGCTGGGCAAGCTTAAGGTCTTTGATGGTGGATCTCTTCTTCGTGAGAAGTTATTGGAGACAAATATGAGTGGCTTAACTGGTCATGTTGAATTTGATCAAGACAGAAGCCGTGCGAGTGGCATTTATGATATCATTAATTTTGATCAGATGAAAATTCGTACGGTTGGTTTTTGGACTTATTATTCAGGTTTTTCAGTTTCTCCCCCGGAAACTCTTAAAAGGGGAAGAAGTAGCTATTCTCCACTGGATCAGAAGCTTGACAATGTCACGTGGCCTGGTGGAAATACAGAAAGGCCACGCGGTTGGGAGATTGCCGATAATGAAAAACCATTGAGAATTGGAGTTCCAAAAAGAGTAAGCTTTGTTGATTTTGTTACAGAACGGAACAACAGCCATAAAATCGAAGGATACTGCATTGATGTGTTCAATGAAGCACTGAAGTTAGTCCCGTATGATGTTCCTTACAGATTTGAACCTTTTGGTAATGGCCGGTCCAATCCCAGTTATGATGAGCTTGTGAAGATGGTTGCAGAAAACGTGTTTGATGCAGCTGTTGGGGACATTGCAATTGTGAAAAACCGGACAATGATTGTGGATTTTTCTCAGCCCTATGTTATCACCGGGCTAGTGATAGTGGCACCTATTGACAATTCAAAGTCAAATGCTTGGGTGTTTCTCAAACCATTTTCATTGGAGATGTGGTGCGTTACTGCATCTTTCTTTGTGATAATTGCTGTGGTAATGTGGGTTCTTGAGCATCGAGTCAACAAAGATTTTCGGGGTCCCCCTAAGAGGCAGCTCGCTACAATCTTTACGTTCAGCTTTTCAACCCTATTTAAGAAAAATCAAGAAGATACTGTGAGCCCGCTTGGGCGAATGGTGATGGTGATATGGCTTTTCCTATTGATGGTGATCACGTCAAGCTATACTGCAAGCTTGACTTCAATCCTTACAGTTCAGCAGCTTTCGATACCCATCACTGGAATTGATAGTTTGATTGCAAGCAATTGGCCTATAGGCTACCAAGTAGGGTCATTTGCTTATAGCTATCTGACTGATAGCCTCTACATTCATAGCTCGAGACTTGTTCCACTAGGCTCCCCAGAGGAGTATGAAACTGCACTGCGAAAAGGGCCGGATGATGGAGGGGTGGCAGCTATTATAGATGAGCTTACTTATGTTGAATTATTTCTTTCAAGTCAAACTGATTTCGGAATTATTGGTGACACATTTACGAGGAGCGGATGGGGATTTGCCTTTCCAAGAGATTCTCCCCTTGCTGTTGACATTTCCTCTGCAATCTTGAAACTTTCGGAGAGTGGAACACTTCAGAAGATTCATGAGAAATGGTTCTGCAAGATGGGTTGTCGTGGTGACAAGAAGCTGGAGTCCGACGAGTCTAACCAACTCCACTTGATAAGCTTCTGGGGTCTATACCTATTATGTGGCGCCTTCACTCTCGCTGCGCTTGTAATCTTTCTGCTGCGAGTGATACACCAATTTGTCCGCTACAAGAAGCAGCGCGCAAACCATCTCTCTTCATCGTTGTCATCAAGCTCGTGGAGTTCTCGATGGTCTCAGGTCATGAACTTTGTTGACTTCATCGACGAGAAGGAAGAAGCAATAAAAAAAATGTACGCTCAGGGTGACAGTACTCAAGGTCAGGTTAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009506 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0030054 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055044 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

913

Amino Acids

102.48

Weight (kDa)

6.38

Isoelectric Point (pI)

37.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	31 - 325	9.5e-13	Periplasmic binding protein
ANF_receptor	PF01094	45 - 403	8.9e-90	Receptor family ligand binding region
SBP_bac_3	PF00497	482 - 806	6.2e-12	Bacterial extracellular solute-binding proteins, family 3
Lig_chan-Glu_bd	PF10613	482 - 568	5.9e-10	Ligated ion channel L-glutamate- and glycine-binding site
Lig_chan	PF00060	585 - 838	1.2e-33	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006374)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32400
fragaria_vesca	FvH4_6g43290 FvH4_6g43290 FvH4_6g43290 FvH4_6g43290
malus_domestica	MD09G1070000.v1.1 MD09G1108300.v1.1 MD17G1096200.v1.1
prunus_persica	Prupe.3G217800_v2.0.a1 Prupe.3G217800_v2.0.a1 Prupe.3G217800_v2.0.a1
pyrus_communis	pycom09g03020 pycom09g03080 pycom17g09230
rosa_chinensis	RchiOBHm_Chr2g0159561
rosa_laevigata	RLG00000021166
rosa_multiflora	Rmu_sc0012414.1_g000014 Rmu_sc0012414.1_g000015
rosa_roxburghii	Rroxscaffold_2G00090400
rosa_rugosa	Rorug02G0476900 Rorug02G0476900
rosa_samantha	Rh2AG543800 Rh2AG544100 Rh2BG556900 Rh2CG527100 Rh2DG565900
rosa_wichuraiana	Rw2G044980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	1702, 2513
AccBSI	CCGCTC	1 cut(s)	2307
AccII	CGCG	2 cut(s)	1365, 2589
AciI	CCGC	4 cut(s)	1365, 1931, 2307, 2572
AclWI	GGATC	7 cut(s)	240, 253, 374, 674, 680, 1102, 1320
AcoI	YGGCCR	1 cut(s)	1552
AcsI	RAATTY	6 cut(s)	183, 226, 988, 1059, 1154, 1218
AcuI	CTGAAG	2 cut(s)	1523, 2378
AcvI	CACGTG	1 cut(s)	1335
AcyI	GRCGYC	1 cut(s)	2514
AfaI	GTAC	4 cut(s)	354, 1225, 2702, 2719
AfiI	CCNNNNNNNGG	5 cut(s)	690, 1857, 1972, 2045, 2207
AflII	CTTAAG	2 cut(s)	212, 1076
AflIII	ACRYGT	1 cut(s)	1602
AjiI	CACGTC	1 cut(s)	1983
AjnI	CCWGG	1 cut(s)	1339
Alw26I	GTCTC	5 cut(s)	350, 1042, 1115, 2140, 2646
AlwI	GGATC	7 cut(s)	240, 253, 374, 674, 680, 1102, 1320
Ama87I	CYCGRG	1 cut(s)	2143
AoxI	GGCC	6 cut(s)	652, 1337, 1358, 1552, 2075, 2198
ApoI	RAATTY	6 cut(s)	183, 226, 988, 1059, 1154, 1218
AseI	ATTAAT	1 cut(s)	1200
Asp700I	GAANNNNTTC	3 cut(s)	258, 2253, 2414
AspA2I	CCTAGG	1 cut(s)	689
AspLEI	GCGC	4 cut(s)	2516, 2533, 2589, 2591
AspS9I	GGNCC	5 cut(s)	296, 653, 1557, 1847, 2198
AsuC2I	CCSGG	2 cut(s)	1271, 1687
AsuHPI	GGTGA	9 cut(s)	97, 431, 1675, 1957, 1963, 1987, 2300, 2450, 2723
AvaI	CYCGRG	1 cut(s)	2143
AvaII	GGWCC	3 cut(s)	296, 1557, 1847
AvrII	CCTAGG	1 cut(s)	689
BamHI	GGATCC	1 cut(s)	245
BanI	GGYRCC	2 cut(s)	1702, 2513
BanII	GRGCYC	1 cut(s)	1931
BauI	CACGAG	2 cut(s)	657, 2623
BbrPI	CACGTG	1 cut(s)	1335
BbvCI	CCTCAGC	1 cut(s)	203
BceAI	ACGGC	1 cut(s)	1158
BciT130I	CCWGG	1 cut(s)	1341
BciVI	GTATCC	1 cut(s)	1466
BclI	TGATCA	3 cut(s)	1159, 1207, 1977
BcnI	CCSGG	2 cut(s)	1271, 1687
BcoDI	GTCTC	5 cut(s)	350, 1042, 1115, 2140, 2646
BfaI	CTAG	6 cut(s)	54, 231, 690, 885, 1691, 2159
BfmI	CTRYAG	3 cut(s)	12, 774, 2079
BfoI	RGCGCY	1 cut(s)	2517
BfrI	CTTAAG	2 cut(s)	212, 1076
BfuI	GTATCC	1 cut(s)	1466
BlnI	CCTAGG	1 cut(s)	689
BlpI	GCTNAGC	1 cut(s)	913
BmcAI	AGTACT	1 cut(s)	2719
Bme1390I	CCNGG	3 cut(s)	1271, 1341, 1687
Bme18I	GGWCC	3 cut(s)	296, 1557, 1847
BmeT110I	CYCGRG	1 cut(s)	2143
BmgBI	CACGTC	1 cut(s)	1983
BmgT120I	GGNCC	5 cut(s)	296, 653, 1557, 1847, 2198
BmiI	GGNNCC	6 cut(s)	247, 1704, 1848, 1849, 2164, 2515
BmrFI	CCNGG	3 cut(s)	1271, 1341, 1687
BmrI	ACTGGG	1 cut(s)	1560
BmsI	GCATC	4 cut(s)	424, 1600, 1787, 1831
BmuI	ACTGGG	1 cut(s)	1560
BplI	GAGNNNNNCTC	2 cut(s)	1039, 1071
BpmI	CTGGAG	1 cut(s)	2471
Bpu10I	CCTNAGC	2 cut(s)	203, 2706
Bpu1102I	GCTNAGC	1 cut(s)	913
BpuEI	CTTGAG	2 cut(s)	1838, 2706
BpuMI	CCSGG	2 cut(s)	1271, 1687
BsaAI	YACGTR	2 cut(s)	842, 1335
BsaBI	GATNNNNATC	3 cut(s)	684, 1976, 2039
BsaHI	GRCGYC	1 cut(s)	2514
BsaI	GGTCTC	3 cut(s)	350, 1042, 2646
BsaJI	CCNNGG	3 cut(s)	144, 689, 1269
BsaWI	WCCGGW	1 cut(s)	1644
BsaXI	ACNNNNNCTCC	4 cut(s)	191, 221, 1751, 1781
Bsc4I	CCNNNNNNNGG	5 cut(s)	690, 1857, 1972, 2045, 2207
Bse118I	RCCGGY	1 cut(s)	1554
Bse1I	ACTGG	5 cut(s)	861, 1147, 1314, 1566, 2050
Bse3DI	GCAATG	2 cut(s)	618, 1626
Bse8I	GATNNNNATC	3 cut(s)	684, 1976, 2039
BseBI	CCWGG	1 cut(s)	1341
BseDI	CCNNGG	3 cut(s)	144, 689, 1269
BseGI	GGATG	4 cut(s)	514, 913, 2209, 2315
BseJI	GATNNNNATC	3 cut(s)	684, 1976, 2039
BseLI	CCNNNNNNNGG	5 cut(s)	690, 1857, 1972, 2045, 2207
BseMI	GCAATG	2 cut(s)	618, 1626
BseMII	CTCAG	6 cut(s)	194, 202, 915, 1682, 2657, 2720
BseNI	ACTGG	5 cut(s)	861, 1147, 1314, 1566, 2050
BsePI	GCGCGC	1 cut(s)	2587
BseRI	GAGGAG	2 cut(s)	2186, 2317
BseYI	CCCAGC	1 cut(s)	1066
BsgI	GTGCAG	2 cut(s)	138, 2169
Bsh1236I	CGCG	2 cut(s)	1365, 2589
BshFI	GGCC	6 cut(s)	654, 1339, 1360, 1554, 2077, 2200
BshNI	GGYRCC	2 cut(s)	1702, 2513
BsiHKCI	CYCGRG	1 cut(s)	2143
BsiSI	CCGG	5 cut(s)	1271, 1555, 1645, 1686, 2201
BsiWI	CGTACG	1 cut(s)	1223
BslFI	GGGAC	4 cut(s)	208, 1496, 1637, 1833
BslI	CCNNNNNNNGG	5 cut(s)	690, 1857, 1972, 2045, 2207
BsmAI	GTCTC	5 cut(s)	350, 1042, 1115, 2140, 2646
BsmFI	GGGAC	4 cut(s)	208, 1496, 1637, 1833
BsnI	GGCC	6 cut(s)	654, 1339, 1360, 1554, 2077, 2200
Bso31I	GGTCTC	3 cut(s)	350, 1042, 2646
BsoBI	CYCGRG	1 cut(s)	2143
Bsp1286I	GDGCHC	1 cut(s)	1931
Bsp1720I	GCTNAGC	1 cut(s)	913
Bsp19I	CCATGG	1 cut(s)	144
BspACI	CCGC	4 cut(s)	1365, 1931, 2307, 2572
BspANI	GGCC	6 cut(s)	654, 1339, 1360, 1554, 2077, 2200
BspCNI	CTCAG	6 cut(s)	195, 201, 916, 1681, 2656, 2719
BspFNI	CGCG	2 cut(s)	1365, 2589
BspHI	TCATGA	2 cut(s)	2404, 2649
BspLI	GGNNCC	6 cut(s)	247, 1704, 1848, 1849, 2164, 2515
BspPI	GGATC	7 cut(s)	240, 253, 374, 674, 680, 1102, 1320
BspT107I	GGYRCC	2 cut(s)	1702, 2513
BspTI	CTTAAG	2 cut(s)	212, 1076
BspTNI	GGTCTC	3 cut(s)	350, 1042, 2646
BsrBI	CCGCTC	1 cut(s)	2307
BsrDI	GCAATG	2 cut(s)	618, 1626
BsrFI	RCCGGY	1 cut(s)	1554
BsrI	ACTGG	5 cut(s)	861, 1147, 1314, 1566, 2050
BssAI	RCCGGY	1 cut(s)	1554
BssECI	CCNNGG	3 cut(s)	144, 689, 1269
BssHII	GCGCGC	1 cut(s)	2587
BssNI	GRCGYC	1 cut(s)	2514
BssSI	CACGAG	2 cut(s)	657, 2623
BssT1I	CCWWGG	2 cut(s)	144, 689
Bst2BI	CACGAG	2 cut(s)	657, 2623
Bst2UI	CCWGG	1 cut(s)	1341
Bst4CI	ACNGT	7 cut(s)	709, 778, 961, 1228, 1924, 2020, 2717
Bst6I	CTCTTC	3 cut(s)	313, 1104, 2608
BstACI	GRCGYC	1 cut(s)	2514
BstAFI	CTTAAG	2 cut(s)	212, 1076
BstBAI	YACGTR	2 cut(s)	842, 1335
BstC8I	GCNNGC	7 cut(s)	873, 1073, 1866, 1931, 1999, 2068, 2589
BstDEI	CTNAG	8 cut(s)	188, 203, 913, 924, 1668, 1854, 2643, 2706
BstDSI	CCRYGG	1 cut(s)	144
BstF5I	GGATG	4 cut(s)	514, 913, 2209, 2315
BstFNI	CGCG	2 cut(s)	1365, 2589
BstH2I	RGCGCY	1 cut(s)	2517
BstHHI	GCGC	4 cut(s)	2516, 2533, 2589, 2591
BstMAI	GTCTC	5 cut(s)	350, 1042, 1115, 2140, 2646
BstMWI	GCNNNNNNNGC	7 cut(s)	66, 140, 149, 208, 455, 1072, 2197
BstNI	CCWGG	1 cut(s)	1341
BstSCI	CCNGG	3 cut(s)	1269, 1339, 1685
BstSFI	CTRYAG	3 cut(s)	12, 774, 2079
BstSNI	TACGTA	1 cut(s)	842
BstUI	CGCG	2 cut(s)	1365, 2589
BstX2I	RGATCY	2 cut(s)	245, 1094
BstXI	CCANNNNNNTGG	1 cut(s)	1368
BstYI	RGATCY	2 cut(s)	245, 1094
BsuI	GTATCC	1 cut(s)	1466
BsuRI	GGCC	6 cut(s)	654, 1339, 1360, 1554, 2077, 2200
BtgI	CCRYGG	1 cut(s)	144
BtgZI	GCGATG	1 cut(s)	447
BtrI	CACGTC	1 cut(s)	1983
BtsCI	GGATG	4 cut(s)	514, 913, 2209, 2315
BtsI	GCAGTG	2 cut(s)	23, 2186
BtsIMutI	CAGTG	7 cut(s)	23, 196, 375, 1307, 1499, 2043, 2186
Cac8I	GCNNGC	7 cut(s)	873, 1073, 1866, 1931, 1999, 2068, 2589
CciI	TCATGA	2 cut(s)	2404, 2649
CfoI	GCGC	4 cut(s)	2516, 2533, 2589, 2591
Cfr10I	RCCGGY	1 cut(s)	1554
Cfr13I	GGNCC	5 cut(s)	296, 653, 1557, 1847, 2198
Csp6I	GTAC	4 cut(s)	353, 1224, 2701, 2718
CspCI	CAANNNNNGTGG	2 cut(s)	1638, 1673
CviAII	CATG	7 cut(s)	145, 545, 674, 1045, 1148, 2405, 2650
CviQI	GTAC	4 cut(s)	353, 1224, 2701, 2718
DdeI	CTNAG	8 cut(s)	188, 203, 913, 924, 1668, 1854, 2643, 2706
DinI	GGCGCC	1 cut(s)	2515
DraI	TTTAAA	1 cut(s)	612
EaeI	YGGCCR	1 cut(s)	1552
Eam1104I	CTCTTC	3 cut(s)	313, 1104, 2608
EarI	CTCTTC	3 cut(s)	313, 1104, 2608
Eco105I	TACGTA	1 cut(s)	842
Eco130I	CCWWGG	2 cut(s)	144, 689
Eco24I	GRGCYC	1 cut(s)	1931
Eco31I	GGTCTC	3 cut(s)	350, 1042, 2646
Eco32I	GATATC	1 cut(s)	1195
Eco47I	GGWCC	3 cut(s)	296, 1557, 1847
Eco57I	CTGAAG	2 cut(s)	1523, 2378
Eco72I	CACGTG	1 cut(s)	1335
Eco88I	CYCGRG	1 cut(s)	2143
EcoO109I	RGGNCCY	1 cut(s)	1847
EcoRI	GAATTC	1 cut(s)	183
EcoRII	CCWGG	1 cut(s)	1339
EcoRV	GATATC	1 cut(s)	1195
EcoT14I	CCWWGG	2 cut(s)	144, 689
EcoT38I	GRGCYC	1 cut(s)	1931
EgeI	GGCGCC	1 cut(s)	2515
EheI	GGCGCC	1 cut(s)	2515
ErhI	CCWWGG	2 cut(s)	144, 689
FaeI	CATG	7 cut(s)	148, 548, 677, 1048, 1151, 2408, 2653
FalI	AAGNNNNNCTT	4 cut(s)	342, 374, 1990, 2022
FaqI	GGGAC	4 cut(s)	208, 1496, 1637, 1833
FatI	CATG	7 cut(s)	144, 544, 673, 1044, 1147, 2404, 2649
FauI	CCCGC	1 cut(s)	1938
FauNDI	CATATG	3 cut(s)	321, 367, 726
FbaI	TGATCA	3 cut(s)	1159, 1207, 1977
FokI	GGATG	4 cut(s)	521, 920, 2216, 2322
FriOI	GRGCYC	1 cut(s)	1931
FspBI	CTAG	6 cut(s)	54, 231, 690, 885, 1691, 2159
GlaI	GCGC	4 cut(s)	2515, 2532, 2588, 2590
GsaI	CCCAGC	1 cut(s)	1070
GsuI	CTGGAG	1 cut(s)	2471
HaeII	RGCGCY	1 cut(s)	2517
HaeIII	GGCC	6 cut(s)	654, 1339, 1360, 1554, 2077, 2200
HapII	CCGG	5 cut(s)	1271, 1555, 1645, 1686, 2201
HhaI	GCGC	4 cut(s)	2516, 2533, 2589, 2591
Hin1I	GRCGYC	1 cut(s)	2514
Hin1II	CATG	7 cut(s)	148, 548, 677, 1048, 1151, 2408, 2653
Hin6I	GCGC	4 cut(s)	2514, 2531, 2587, 2589
HinP1I	GCGC	4 cut(s)	2514, 2531, 2587, 2589
HincII	GTYRAC	3 cut(s)	1831, 2350, 2662
HindII	GTYRAC	3 cut(s)	1831, 2350, 2662
HindIII	AAGCTT	6 cut(s)	255, 1073, 1319, 1423, 1999, 2485
HinfI	GANTC	8 cut(s)	699, 782, 857, 1827, 2333, 2401, 2453, 2462
HpaII	CCGG	5 cut(s)	1271, 1555, 1645, 1686, 2201
HphI	GGTGA	9 cut(s)	97, 431, 1675, 1957, 1963, 1987, 2300, 2450, 2723
Hpy166II	GTNNAC	3 cut(s)	1831, 2350, 2662
Hpy8I	GTNNAC	3 cut(s)	1831, 2350, 2662
Hpy99I	CGWCG	2 cut(s)	2462, 2675
HpyAV	CCTTC	5 cut(s)	87, 830, 1463, 2527, 2671
HpyCH4III	ACNGT	7 cut(s)	709, 778, 961, 1228, 1924, 2020, 2717
HpyCH4IV	ACGT	5 cut(s)	841, 1334, 1602, 1880, 1982
HpyF10VI	GCNNNNNNNGC	7 cut(s)	66, 140, 149, 208, 455, 1072, 2197
HpyF3I	CTNAG	8 cut(s)	188, 203, 913, 924, 1668, 1854, 2643, 2706
HpySE526I	ACGT	5 cut(s)	841, 1334, 1602, 1880, 1982
Hsp92I	GRCGYC	1 cut(s)	2514
Hsp92II	CATG	7 cut(s)	148, 548, 677, 1048, 1151, 2408, 2653
HspAI	GCGC	4 cut(s)	2514, 2531, 2587, 2589
KasI	GGCGCC	1 cut(s)	2513
KflI	GGGWCCC	1 cut(s)	1847
Ksp22I	TGATCA	3 cut(s)	1159, 1207, 1977
LmnI	GCTCC	4 cut(s)	95, 199, 2168, 2304
LweI	GCATC	4 cut(s)	424, 1600, 1787, 1831
MaeI	CTAG	6 cut(s)	54, 231, 690, 885, 1691, 2159
MaeII	ACGT	5 cut(s)	841, 1334, 1602, 1880, 1982
MaeIII	GTNAC	7 cut(s)	832, 1330, 1438, 1771, 2288, 2438, 2711
MbiI	CCGCTC	1 cut(s)	2307
MboII	GAAGA	9 cut(s)	300, 1091, 1094, 1302, 1597, 1928, 2410, 2595, 2693
MfeI	CAATTG	2 cut(s)	1632, 2071
MflI	RGATCY	2 cut(s)	245, 1094
MhlI	GDGCHC	1 cut(s)	1931
Mly113I	GGCGCC	1 cut(s)	2514
MlyI	GAGTC	4 cut(s)	866, 1836, 2462, 2471
MmeI	TCCRAC	1 cut(s)	2481
MroXI	GAANNNNTTC	3 cut(s)	258, 2253, 2414
MslI	CAYNNNNRTG	1 cut(s)	1484
MspA1I	CMGCKG	2 cut(s)	1066, 1616
MspCI	CTTAAG	2 cut(s)	212, 1076
MspI	CCGG	5 cut(s)	1271, 1555, 1645, 1686, 2201
MspR9I	CCNGG	3 cut(s)	1271, 1341, 1687
MunI	CAATTG	2 cut(s)	1632, 2071
MvaI	CCWGG	1 cut(s)	1341
MvnI	CGCG	2 cut(s)	1365, 2589
MwoI	GCNNNNNNNGC	7 cut(s)	66, 140, 149, 208, 455, 1072, 2197
NarI	GGCGCC	1 cut(s)	2514
NciI	CCSGG	2 cut(s)	1271, 1687
NcoI	CCATGG	1 cut(s)	144
NdeI	CATATG	3 cut(s)	321, 367, 726
NlaIII	CATG	7 cut(s)	148, 548, 677, 1048, 1151, 2408, 2653
NlaIV	GGNNCC	6 cut(s)	247, 1704, 1848, 1849, 2164, 2515
NmuCI	GTSAC	4 cut(s)	1330, 2288, 2438, 2711
PaeR7I	CTCGAG	1 cut(s)	2143
PagI	TCATGA	2 cut(s)	2404, 2649
PauI	GCGCGC	1 cut(s)	2587
PdmI	GAANNNNTTC	3 cut(s)	258, 2253, 2414
PfeI	GAWTC	4 cut(s)	699, 782, 2333, 2401
Pfl23II	CGTACG	1 cut(s)	1223
PflFI	GACNNNGTC	1 cut(s)	1328
PleI	GAGTC	4 cut(s)	865, 1835, 2461, 2470
PluTI	GGCGCC	1 cut(s)	2517
PmaCI	CACGTG	1 cut(s)	1335
PmlI	CACGTG	1 cut(s)	1335
PpsI	GAGTC	4 cut(s)	865, 1835, 2461, 2470
Ppu21I	YACGTR	2 cut(s)	842, 1335
PpuMI	RGGWCCY	1 cut(s)	1847
PshBI	ATTAAT	1 cut(s)	1200
Psp5II	RGGWCCY	1 cut(s)	1847
Psp6I	CCWGG	1 cut(s)	1339
PspCI	CACGTG	1 cut(s)	1335
PspFI	CCCAGC	1 cut(s)	1066
PspGI	CCWGG	1 cut(s)	1339
PspLI	CGTACG	1 cut(s)	1223
PspN4I	GGNNCC	6 cut(s)	247, 1704, 1848, 1849, 2164, 2515
PspPI	GGNCC	5 cut(s)	296, 653, 1557, 1847, 2198
PspPPI	RGGWCCY	1 cut(s)	1847
PsrI	GAACNNNNNNTAC	2 cut(s)	1797, 1829
PsuI	RGATCY	2 cut(s)	245, 1094
PsyI	GACNNNGTC	1 cut(s)	1328
PteI	GCGCGC	1 cut(s)	2587
PvuII	CAGCTG	2 cut(s)	1066, 1616
RsaI	GTAC	4 cut(s)	354, 1225, 2702, 2719
RsaNI	GTAC	4 cut(s)	353, 1224, 2701, 2718
RseI	CAYNNNNRTG	1 cut(s)	1484
Sau96I	GGNCC	5 cut(s)	296, 653, 1557, 1847, 2198
ScaI	AGTACT	1 cut(s)	2719
SchI	GAGTC	4 cut(s)	866, 1836, 2462, 2471
ScrFI	CCNGG	3 cut(s)	1271, 1341, 1687
SduI	GDGCHC	1 cut(s)	1931
SfaNI	GCATC	4 cut(s)	424, 1600, 1787, 1831
SfcI	CTRYAG	3 cut(s)	12, 774, 2079
SfoI	GGCGCC	1 cut(s)	2515
Sfr274I	CTCGAG	1 cut(s)	2143
SinI	GGWCC	3 cut(s)	296, 1557, 1847
SlaI	CTCGAG	1 cut(s)	2143
SmiI	ATTTAAAT	1 cut(s)	612
SmiMI	CAYNNNNRTG	1 cut(s)	1484
SmlI	CTYRAG	5 cut(s)	212, 1076, 1817, 2143, 2721
SmoI	CTYRAG	5 cut(s)	212, 1076, 1817, 2143, 2721
SnaBI	TACGTA	1 cut(s)	842
SsiI	CCGC	4 cut(s)	1365, 1931, 2307, 2572
SspDI	GGCGCC	1 cut(s)	2513
SspI	AATATT	1 cut(s)	91
SspMI	CTAG	6 cut(s)	54, 231, 690, 885, 1691, 2159
StyD4I	CCNGG	3 cut(s)	1269, 1339, 1685
StyI	CCWWGG	2 cut(s)	144, 689
SwaI	ATTTAAAT	1 cut(s)	612
TaaI	ACNGT	7 cut(s)	709, 778, 961, 1228, 1924, 2020, 2717
TaiI	ACGT	5 cut(s)	844, 1337, 1605, 1883, 1985
TaqI	TCGA	8 cut(s)	865, 980, 1467, 1825, 2033, 2144, 2635, 2670
TatI	WGTACW	1 cut(s)	2717
TfiI	GAWTC	4 cut(s)	699, 782, 2333, 2401
TscAI	CASTG	7 cut(s)	30, 196, 382, 1314, 1506, 2050, 2193
TseFI	GTSAC	4 cut(s)	1330, 2288, 2438, 2711
Tsp45I	GTSAC	4 cut(s)	1330, 2288, 2438, 2711
TspGWI	ACGGA	3 cut(s)	212, 238, 1462
TspRI	CASTG	7 cut(s)	30, 196, 382, 1314, 1506, 2050, 2193
Tth111I	GACNNNGTC	1 cut(s)	1328
Vha464I	CTTAAG	2 cut(s)	212, 1076
VpaK11BI	GGWCC	3 cut(s)	296, 1557, 1847
VspI	ATTAAT	1 cut(s)	1200
XapI	RAATTY	6 cut(s)	183, 226, 988, 1059, 1154, 1218
XhoI	CTCGAG	1 cut(s)	2143
XmaJI	CCTAGG	1 cut(s)	689
XmnI	GAANNNNTTC	3 cut(s)	258, 2253, 2414
XspI	CTAG	6 cut(s)	54, 231, 690, 885, 1691, 2159
ZrmI	AGTACT	1 cut(s)	2719

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.