Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0006374

Physical Location & Seq

Genomic Coordinates Reverse (-)

8125432 .. 8130366

Gene Structure 4935 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1096200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2739 bp

ATGAGGGGTGCTGTAGTTTTGCCACTGCAATTTCTGATATGGGTTTTCCTCACTGGCTGTTTTTACTGCCAGAGGCCTTCAGTGGTGAATGTTGGAGCAATTTTTACATTCAACTCGGTTATTGGTAGAGTTGCAAAGACAGCCATGGAAGCTGCTCTATCTGATATTAATGCAGATTCAAGAATACTCAGTGGGACGGAGCTGAAGTTGCATATGGAGGATGCAAATTCGAGTGTGTTCTTGGGATCCGTTGAAGCTTTTCAGGTGCTTGACAAAAGTATAGTAGCCATAATTGGACCACAGTCCTCTTCAATAGCTCATATGATCTCTGAAATTGCTAATGGTCTCCAAGTACCTCTTATCTCATATGCTGCCACTGATCCTACTCTCTCTGCTCTCCAATTCCCATTCTTTCTCCGAACCACACAAAGCGATGCTCACCAAATGGCTGCTATGGCTGGTTTAATTAATTTTTATGGGTGGAAGGAGGTTATTGCTGTCTTTGTGGATGATGATTATGGGAGGAATGCAATATCTTCTTTACATGATGAACTTGACAAAATAATGTCAAGGATTTCTTATAAGTTGGCGTTGCCTGTTGATTTTAATCTAAGTGACATTGCTGATTTGCTCAATAAATCCAAATTGCTTGGCCCTCGTGTCTATATTGTTCACGTGGATCCTGATCCCAGGTTGAGAATCTTTACTGTCGCCAAAGAACTTCATATGATGACAAGCAATTATGTGTGGTTTGCAACAGATTGGCTTTCTTCTACAGTAGATTCACTCGCTCCAATGAATCGTACTTCACTCGCTGTCCTTCAAGGTGTAGTTACTCTTCGTAAGCATATCCCAGAGTCAAGGCAAAAGCGTGCATTTATGACTAGGTGGAAAAAAATGCAGCAAGACGGTTTAGCAATATCCGAGTTAAATGTCTATGGACTTTATGCTTATGACACAGTTTGGGCTGTTGCACAATCGATTGAAAATTATATAAATGAATACAGAAATATCTCATTCTCTGTCATTGATAAATTACATGTCATGAAACCATCTCAAATTCAGCTAGGCAAGCTTAAAGTCTTTGATGGTGGATCTCTTCTTCGTGAGAAGCTATTGGAGACAAAAATGAGTGGTTTAACTGGTCACATTGAATTTGATCATGAAAGAAGCCGTGCTATTGGGACTTATGATATCATTAATATTGATCAGATGACAATTCATACGGTTGGTTTTTGGACTAATTATTCAGATTTATCAGTTTCTCCCCCAGAAACTCATAAAGGGAGAAGAAGTAGCTATTCTCCACTGGATCAGAAGCTTGGCAATATCACGTGGCCAGGTGGAAATACAGAAAGACCACGCGGTTGGGAGATTGCAGATAATGAAAAACCGTTGAGAATTGGAGTTCCAAAAAGAGTGAGCTTTGTTGAATTTGTTACAGAACTGAACAACAGCCATAAAATCGAAGGATACTGTATTGATGTGTTCAATGAAGCACGGAAGTTGATCCCATATGATGTTCCTCACAGATTTGAACCTTTTGGTGATGGTCGGTCCAATCCCAGTTATAATGAGCTTGTGAAGATGGTTGCAGAAAAGGTGTTTGATGCAGCTGTTGGGGACATTGCAATTGTGAAAAACCGGACAGTGATGGTGGATTTTTCTCAGCCCTATGCTACGACCGGGCTAGTGATAGTGGCTCCTATTGACAATTCGAAGTCAAATGCTTGGGTTTTTCTCAAACCATTTTCATTGGAGATGTGGTGTGTCACTGCATCTTTCTTTGTGATAATTGCTGTGGTAATGTGGATTCTTGAGCATCGAGTCAACAAAGATTTTCGGGGTCCCCCTAAGAGGCAGATCGTTACAATGTTTCTGTTCAGCTTCTCAACACTTTTTAAGAAAAACCAAGAAGATACCGTGAGCCCGCTTGGGCGGATGGTGATGGTGGTATGGCTTTTCCTATTGATGGTGATCACATCAAGCTATACTGCAAGCTTGACTTCAATCCTTACAGTTCAGCAGCTTTCGTCACCCATCACTGGAATTGATAGTTTGATTGCAAGCAATTGGCCTATAGGGTACCAAGTAGGGTCATTTGCTTATGGCTATCTGACTGATGGCCTCTACATTCCTAGGTCAAGACTTGTTCCACTAGGCTCCCCAGAAGAGTATGAAAATGCACTGCGGAAAGGACCGGATGATGGAGGGGTGGCAGCTATTATAGATGAGCTTACTTATGTTGAATTATTTCTTTCAAGTCAAACTGATTTCGGAATTATTGGGCAACCATTTACGAGGAGCGGATGGGGGTTTGCTTTTCCAAGAGATTCTCCCCTTGCTATTGACATGTCCACTGCAATCTTGAAACTTTCGGAGAATGGAGCACTTCAGAAGATTCACGAGAAATGGTTCTGCAAGACGGGCTGTCTTAATAAGAATCTGGACTCTGATGAGTCTAACCAACTCTACTTGATCAGCTTCTGGGGTCTATACCTATTATGTGGCAGCGTCACTCTCGCTGCGCTTATAATCTTTCTGCTAAGAGCAGTTCACCAATTTGTGCGCTACAAGAAACAGCACGCAAACCTGCCGTCATCTTCTTTATCCTCAAGCTCATGGAGTTCTCGATGGTCTCAGGTCCTGGTCAACTTTGTTGACTTCATTGACGAGAAGGAAGAAGCTATCAAAAAAATGTTTGCTCAGGGTGACAGTACTCAAGGTCAGGTTAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009506 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0030054 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055044 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

913

Amino Acids

102.25

Weight (kDa)

6.44

Isoelectric Point (pI)

41.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	31 - 327	1.3e-14	Periplasmic binding protein
ANF_receptor	PF01094	45 - 403	3.3e-88	Receptor family ligand binding region
SBP_bac_3	PF00497	480 - 806	5.9e-11	Bacterial extracellular solute-binding proteins, family 3
Lig_chan-Glu_bd	PF10613	481 - 568	6.9e-11	Ligated ion channel L-glutamate- and glycine-binding site
Lig_chan	PF00060	585 - 837	1.3e-34	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006374)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32400
fragaria_vesca	FvH4_6g43290 FvH4_6g43290 FvH4_6g43290 FvH4_6g43290
malus_domestica	MD09G1070000.v1.1 MD09G1108300.v1.1 MD17G1096200.v1.1
prunus_persica	Prupe.3G217800_v2.0.a1 Prupe.3G217800_v2.0.a1 Prupe.3G217800_v2.0.a1
pyrus_communis	pycom09g03020 pycom09g03080 pycom17g09230
rosa_chinensis	RchiOBHm_Chr2g0159561
rosa_laevigata	RLG00000021166
rosa_multiflora	Rmu_sc0012414.1_g000014 Rmu_sc0012414.1_g000015
rosa_roxburghii	Rroxscaffold_2G00090400
rosa_rugosa	Rorug02G0476900 Rorug02G0476900
rosa_samantha	Rh2AG543800 Rh2AG544100 Rh2BG556900 Rh2CG527100 Rh2DG565900
rosa_wichuraiana	Rw2G044980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	582, 1572, 2534
Acc36I	ACCTGC	1 cut(s)	2601
Acc65I	GGTACC	1 cut(s)	2085
AccB1I	GGYRCC	1 cut(s)	2085
AccBSI	CCGCTC	1 cut(s)	2307
AccII	CGCG	1 cut(s)	1365
AciI	CCGC	5 cut(s)	1365, 1931, 1939, 2191, 2307
AclWI	GGATC	9 cut(s)	240, 253, 374, 674, 680, 687, 1102, 1320, 1504
AcoI	YGGCCR	1 cut(s)	1337
AcsI	RAATTY	4 cut(s)	226, 1059, 1154, 1433
AcuI	CTGAAG	3 cut(s)	63, 224, 2378
AcvI	CACGTG	2 cut(s)	676, 1335
AfaI	GTAC	4 cut(s)	354, 805, 2087, 2719
AfiI	CCNNNNNNNGG	4 cut(s)	1857, 1972, 2045, 2207
AflIII	ACRYGT	2 cut(s)	1039, 2352
AhdI	GACNNNNNGTC	1 cut(s)	2430
AjnI	CCWGG	3 cut(s)	689, 1339, 2646
Alw21I	GWGCWC	1 cut(s)	2392
Alw26I	GTCTC	3 cut(s)	350, 1115, 2643
AlwI	GGATC	9 cut(s)	240, 253, 374, 674, 680, 687, 1102, 1320, 1504
AlwNI	CAGNNNCTG	2 cut(s)	2487, 2647
AoxI	GGCC	5 cut(s)	74, 652, 1337, 2075, 2125
ApeKI	GCWGC	9 cut(s)	152, 371, 449, 901, 1613, 2026, 2219, 2511, 2525
ApoI	RAATTY	4 cut(s)	226, 1059, 1154, 1433
ArsI	GACNNNNNNTTYG	2 cut(s)	2582, 2614
AseI	ATTAAT	3 cut(s)	168, 468, 1200
Asp700I	GAANNNNTTC	3 cut(s)	258, 2253, 2414
Asp718I	GGTACC	1 cut(s)	2085
AspA2I	CCTAGG	1 cut(s)	2138
AspLEI	GCGC	2 cut(s)	2530, 2571
AspS9I	GGNCC	6 cut(s)	296, 653, 1557, 1847, 2198, 2644
AsuC2I	CCSGG	1 cut(s)	1687
AsuHPI	GGTGA	8 cut(s)	97, 431, 1559, 1957, 1987, 2028, 2549, 2723
AsuII	TTCGAA	1 cut(s)	1718
AvaII	GGWCC	5 cut(s)	296, 1557, 1847, 2198, 2644
AvrII	CCTAGG	1 cut(s)	2138
BalI	TGGCCA	1 cut(s)	1339
BamHI	GGATCC	2 cut(s)	245, 679
BanI	GGYRCC	1 cut(s)	2085
BanII	GRGCYC	1 cut(s)	1931
BarI	GAAGNNNNNNTAC	2 cut(s)	822, 854
BauI	CACGAG	2 cut(s)	657, 2405
BbrPI	CACGTG	2 cut(s)	676, 1335
Bbv12I	GWGCWC	1 cut(s)	2392
BbvI	GCAGC	9 cut(s)	139, 358, 436, 913, 1625, 2038, 2231, 2512, 2523
BceAI	ACGGC	2 cut(s)	1158, 2581
BciT130I	CCWGG	3 cut(s)	691, 1341, 2648
BciVI	GTATCC	1 cut(s)	1466
BclI	TGATCA	4 cut(s)	1159, 1207, 1977, 2478
BcnI	CCSGG	1 cut(s)	1687
BcoDI	GTCTC	3 cut(s)	350, 1115, 2643
BfaI	CTAG	5 cut(s)	885, 1067, 1691, 2139, 2159
BfmI	CTRYAG	3 cut(s)	12, 774, 2079
BfuAI	ACCTGC	1 cut(s)	2601
BfuI	GTATCC	1 cut(s)	1466
BisI	GCNGC	9 cut(s)	153, 372, 450, 902, 1614, 2027, 2220, 2512, 2526
BlnI	CCTAGG	1 cut(s)	2138
BlsI	GCNGC	9 cut(s)	154, 373, 451, 903, 1615, 2028, 2221, 2513, 2527
BmcAI	AGTACT	1 cut(s)	2719
Bme1390I	CCNGG	4 cut(s)	691, 1341, 1687, 2648
Bme18I	GGWCC	5 cut(s)	296, 1557, 1847, 2198, 2644
BmeRI	GACNNNNNGTC	1 cut(s)	2430
BmgT120I	GGNCC	6 cut(s)	296, 653, 1557, 1847, 2198, 2644
BmiI	GGNNCC	7 cut(s)	247, 681, 1704, 1848, 1849, 2087, 2164
BmrFI	CCNGG	4 cut(s)	691, 1341, 1687, 2648
BmrI	ACTGGG	1 cut(s)	1560
BmsI	GCATC	5 cut(s)	211, 424, 1600, 1787, 1831
BmuI	ACTGGG	1 cut(s)	1560
BoxI	GACNNNNGTC	1 cut(s)	301
Bpu10I	CCTNAGC	1 cut(s)	2706
Bpu14I	TTCGAA	1 cut(s)	1718
BpuEI	CTTGAG	3 cut(s)	1838, 2599, 2706
BpuMI	CCSGG	1 cut(s)	1687
Bsa29I	ATCGAT	1 cut(s)	980
BsaAI	YACGTR	2 cut(s)	676, 1335
BsaBI	GATNNNNATC	3 cut(s)	606, 684, 1976
BsaI	GGTCTC	2 cut(s)	350, 2643
BsaJI	CCNNGG	3 cut(s)	144, 689, 2138
BsaWI	WCCGGW	2 cut(s)	1644, 2200
BsaXI	ACNNNNNCTCC	6 cut(s)	191, 221, 1279, 1309, 1751, 1781
Bsc4I	CCNNNNNNNGG	4 cut(s)	1857, 1972, 2045, 2207
Bse1I	ACTGG	5 cut(s)	58, 1147, 1314, 1566, 2050
Bse3DI	GCAATG	2 cut(s)	618, 1626
Bse8I	GATNNNNATC	3 cut(s)	606, 684, 1976
BseBI	CCWGG	3 cut(s)	691, 1341, 2648
BseCI	ATCGAT	1 cut(s)	980
BseDI	CCNNGG	3 cut(s)	144, 689, 2138
BseGI	GGATG	5 cut(s)	226, 514, 1947, 2209, 2315
BseJI	GATNNNNATC	3 cut(s)	606, 684, 1976
BseLI	CCNNNNNNNGG	4 cut(s)	1857, 1972, 2045, 2207
BseMI	GCAATG	2 cut(s)	618, 1626
BseMII	CTCAG	4 cut(s)	202, 1682, 2654, 2720
BseNI	ACTGG	5 cut(s)	58, 1147, 1314, 1566, 2050
BseRI	GAGGAG	1 cut(s)	2317
BseXI	GCAGC	9 cut(s)	139, 358, 436, 913, 1625, 2038, 2231, 2512, 2523
Bsh1236I	CGCG	1 cut(s)	1365
Bsh1285I	CGRYCG	1 cut(s)	1686
BshFI	GGCC	5 cut(s)	76, 654, 1339, 2077, 2127
BshNI	GGYRCC	1 cut(s)	2085
BshVI	ATCGAT	1 cut(s)	980
BsiEI	CGRYCG	1 cut(s)	1686
BsiHKAI	GWGCWC	1 cut(s)	2392
BsiSI	CCGG	3 cut(s)	1645, 1686, 2201
BslFI	GGGAC	4 cut(s)	208, 1198, 1637, 1833
BslI	CCNNNNNNNGG	4 cut(s)	1857, 1972, 2045, 2207
BsmAI	GTCTC	3 cut(s)	350, 1115, 2643
BsmFI	GGGAC	4 cut(s)	208, 1198, 1637, 1833
BsmI	GAATGC	1 cut(s)	532
BsnI	GGCC	5 cut(s)	76, 654, 1339, 2077, 2127
Bso31I	GGTCTC	2 cut(s)	350, 2643
Bsp119I	TTCGAA	1 cut(s)	1718
Bsp1286I	GDGCHC	2 cut(s)	1931, 2392
Bsp19I	CCATGG	1 cut(s)	144
BspACI	CCGC	5 cut(s)	1365, 1931, 1939, 2191, 2307
BspANI	GGCC	5 cut(s)	76, 654, 1339, 2077, 2127
BspCNI	CTCAG	4 cut(s)	201, 1681, 2653, 2719
BspDI	ATCGAT	1 cut(s)	980
BspFNI	CGCG	1 cut(s)	1365
BspHI	TCATGA	2 cut(s)	1044, 1162
BspLI	GGNNCC	7 cut(s)	247, 681, 1704, 1848, 1849, 2087, 2164
BspMI	ACCTGC	1 cut(s)	2601
BspPI	GGATC	9 cut(s)	240, 253, 374, 674, 680, 687, 1102, 1320, 1504
BspT104I	TTCGAA	1 cut(s)	1718
BspT107I	GGYRCC	1 cut(s)	2085
BspTNI	GGTCTC	2 cut(s)	350, 2643
BsrBI	CCGCTC	1 cut(s)	2307
BsrDI	GCAATG	2 cut(s)	618, 1626
BsrI	ACTGG	5 cut(s)	58, 1147, 1314, 1566, 2050
BssECI	CCNNGG	3 cut(s)	144, 689, 2138
BssSI	CACGAG	2 cut(s)	657, 2405
BssT1I	CCWWGG	2 cut(s)	144, 2138
Bst2BI	CACGAG	2 cut(s)	657, 2405
Bst2UI	CCWGG	3 cut(s)	691, 1341, 2648
Bst6I	CTCTTC	4 cut(s)	313, 843, 1104, 2166
BstBAI	YACGTR	2 cut(s)	676, 1335
BstBI	TTCGAA	1 cut(s)	1718
BstC8I	GCNNGC	6 cut(s)	873, 1073, 1931, 1999, 2068, 2586
BstDEI	CTNAG	7 cut(s)	188, 611, 1668, 1854, 2546, 2640, 2706
BstDSI	CCRYGG	1 cut(s)	144
BstF5I	GGATG	5 cut(s)	226, 514, 1947, 2209, 2315
BstFNI	CGCG	1 cut(s)	1365
BstHHI	GCGC	2 cut(s)	2530, 2571
BstMAI	GTCTC	3 cut(s)	350, 1115, 2643
BstMCI	CGRYCG	1 cut(s)	1686
BstMWI	GCNNNNNNNGC	6 cut(s)	140, 149, 208, 455, 1072, 2427
BstNI	CCWGG	3 cut(s)	691, 1341, 2648
BstNSI	RCATGY	2 cut(s)	1043, 2356
BstPAI	GACNNNNGTC	1 cut(s)	301
BstSCI	CCNGG	4 cut(s)	689, 1339, 1685, 2646
BstSFI	CTRYAG	3 cut(s)	12, 774, 2079
BstUI	CGCG	1 cut(s)	1365
BstV1I	GCAGC	9 cut(s)	139, 358, 436, 913, 1625, 2038, 2231, 2512, 2523
BstX2I	RGATCY	3 cut(s)	245, 679, 1094
BstXI	CCANNNNNNTGG	1 cut(s)	1368
BstYI	RGATCY	3 cut(s)	245, 679, 1094
Bsu15I	ATCGAT	1 cut(s)	980
BsuI	GTATCC	1 cut(s)	1466
BsuRI	GGCC	5 cut(s)	76, 654, 1339, 2077, 2127
BsuTUI	ATCGAT	1 cut(s)	980
BtgI	CCRYGG	1 cut(s)	144
BtgZI	GCGATG	1 cut(s)	447
BtsCI	GGATG	5 cut(s)	226, 514, 1947, 2209, 2315
BtsI	GCAGTG	4 cut(s)	23, 1773, 2186, 2358
BveI	ACCTGC	1 cut(s)	2601
Cac8I	GCNNGC	6 cut(s)	873, 1073, 1931, 1999, 2068, 2586
CaiI	CAGNNNCTG	2 cut(s)	2487, 2647
CciI	TCATGA	2 cut(s)	1044, 1162
CfoI	GCGC	2 cut(s)	2530, 2571
Cfr13I	GGNCC	6 cut(s)	296, 653, 1557, 1847, 2198, 2644
ClaI	ATCGAT	1 cut(s)	980
CseI	GACGC	1 cut(s)	2503
Csp6I	GTAC	4 cut(s)	353, 804, 2086, 2718
CviAII	CATG	7 cut(s)	145, 545, 1040, 1045, 1163, 2353, 2622
CviQI	GTAC	4 cut(s)	353, 804, 2086, 2718
DdeI	CTNAG	7 cut(s)	188, 611, 1668, 1854, 2546, 2640, 2706
DriI	GACNNNNNGTC	1 cut(s)	2430
EaeI	YGGCCR	1 cut(s)	1337
Eam1104I	CTCTTC	4 cut(s)	313, 843, 1104, 2166
Eam1105I	GACNNNNNGTC	1 cut(s)	2430
EarI	CTCTTC	4 cut(s)	313, 843, 1104, 2166
EciI	GGCGGA	1 cut(s)	1954
Eco130I	CCWWGG	2 cut(s)	144, 2138
Eco147I	AGGCCT	1 cut(s)	76
Eco24I	GRGCYC	1 cut(s)	1931
Eco31I	GGTCTC	2 cut(s)	350, 2643
Eco32I	GATATC	1 cut(s)	1195
Eco47I	GGWCC	5 cut(s)	296, 1557, 1847, 2198, 2644
Eco57I	CTGAAG	3 cut(s)	63, 224, 2378
Eco72I	CACGTG	2 cut(s)	676, 1335
EcoO109I	RGGNCCY	2 cut(s)	1847, 2644
EcoRII	CCWGG	3 cut(s)	689, 1339, 2646
EcoRV	GATATC	1 cut(s)	1195
EcoT14I	CCWWGG	2 cut(s)	144, 2138
EcoT38I	GRGCYC	1 cut(s)	1931
ErhI	CCWWGG	2 cut(s)	144, 2138
FaeI	CATG	7 cut(s)	148, 548, 1043, 1048, 1166, 2356, 2625
FalI	AAGNNNNNCTT	6 cut(s)	342, 374, 562, 594, 1990, 2022
FaqI	GGGAC	4 cut(s)	208, 1198, 1637, 1833
FatI	CATG	7 cut(s)	144, 544, 1039, 1044, 1162, 2352, 2621
FauI	CCCGC	1 cut(s)	1938
FauNDI	CATATG	5 cut(s)	213, 321, 367, 726, 1516
FbaI	TGATCA	4 cut(s)	1159, 1207, 1977, 2478
Fnu4HI	GCNGC	9 cut(s)	153, 372, 450, 902, 1614, 2027, 2220, 2512, 2526
FokI	GGATG	5 cut(s)	233, 521, 1954, 2216, 2322
FriOI	GRGCYC	1 cut(s)	1931
Fsp4HI	GCNGC	9 cut(s)	153, 372, 450, 902, 1614, 2027, 2220, 2512, 2526
FspBI	CTAG	5 cut(s)	885, 1067, 1691, 2139, 2159
GlaI	GCGC	2 cut(s)	2529, 2570
GluI	GCNGC	9 cut(s)	153, 372, 450, 902, 1614, 2027, 2220, 2512, 2526
HaeIII	GGCC	5 cut(s)	76, 654, 1339, 2077, 2127
HapII	CCGG	3 cut(s)	1645, 1686, 2201
HgaI	GACGC	1 cut(s)	2503
HhaI	GCGC	2 cut(s)	2530, 2571
Hin1II	CATG	7 cut(s)	148, 548, 1043, 1048, 1166, 2356, 2625
Hin6I	GCGC	2 cut(s)	2528, 2569
HinP1I	GCGC	2 cut(s)	2528, 2569
HincII	GTYRAC	3 cut(s)	1831, 2653, 2662
HindII	GTYRAC	3 cut(s)	1831, 2653, 2662
HindIII	AAGCTT	4 cut(s)	255, 1073, 1319, 1999
HpaII	CCGG	3 cut(s)	1645, 1686, 2201
HphI	GGTGA	8 cut(s)	97, 431, 1559, 1957, 1987, 2028, 2549, 2723
Hpy166II	GTNNAC	6 cut(s)	673, 1831, 2358, 2557, 2653, 2662
Hpy8I	GTNNAC	6 cut(s)	673, 1831, 2358, 2557, 2653, 2662
HpyAV	CCTTC	5 cut(s)	87, 478, 830, 1463, 2671
HpyCH4IV	ACGT	2 cut(s)	675, 1334
HpyF10VI	GCNNNNNNNGC	6 cut(s)	140, 149, 208, 455, 1072, 2427
HpyF3I	CTNAG	7 cut(s)	188, 611, 1668, 1854, 2546, 2640, 2706
HpySE526I	ACGT	2 cut(s)	675, 1334
Hsp92II	CATG	7 cut(s)	148, 548, 1043, 1048, 1166, 2356, 2625
HspAI	GCGC	2 cut(s)	2528, 2569
KflI	GGGWCCC	1 cut(s)	1847
KpnI	GGTACC	1 cut(s)	2089
Ksp22I	TGATCA	4 cut(s)	1159, 1207, 1977, 2478
LmnI	GCTCC	7 cut(s)	95, 199, 796, 1708, 2168, 2304, 2387
Lsp1109I	GCAGC	9 cut(s)	139, 358, 436, 913, 1625, 2038, 2231, 2512, 2523
LweI	GCATC	5 cut(s)	211, 424, 1600, 1787, 1831
MaeI	CTAG	5 cut(s)	885, 1067, 1691, 2139, 2159
MaeII	ACGT	2 cut(s)	675, 1334
MaeIII	GTNAC	9 cut(s)	614, 832, 1145, 1438, 1771, 1867, 2034, 2515, 2711
MbiI	CCGCTC	1 cut(s)	2307
MfeI	CAATTG	2 cut(s)	1632, 2071
MflI	RGATCY	3 cut(s)	245, 679, 1094
MhlI	GDGCHC	2 cut(s)	1931, 2392
MlsI	TGGCCA	1 cut(s)	1339
MluNI	TGGCCA	1 cut(s)	1339
MlyI	GAGTC	4 cut(s)	866, 1836, 2444, 2468
MmeI	TCCRAC	1 cut(s)	73
Mox20I	TGGCCA	1 cut(s)	1339
MroXI	GAANNNNTTC	3 cut(s)	258, 2253, 2414
MscI	TGGCCA	1 cut(s)	1339
Msp20I	TGGCCA	1 cut(s)	1339
MspA1I	CMGCKG	1 cut(s)	1616
MspI	CCGG	3 cut(s)	1645, 1686, 2201
MspR9I	CCNGG	4 cut(s)	691, 1341, 1687, 2648
MunI	CAATTG	2 cut(s)	1632, 2071
Mva1269I	GAATGC	1 cut(s)	532
MvaI	CCWGG	3 cut(s)	691, 1341, 2648
MvnI	CGCG	1 cut(s)	1365
MwoI	GCNNNNNNNGC	6 cut(s)	140, 149, 208, 455, 1072, 2427
NciI	CCSGG	1 cut(s)	1687
NcoI	CCATGG	1 cut(s)	144
NdeI	CATATG	5 cut(s)	213, 321, 367, 726, 1516
NlaIII	CATG	7 cut(s)	148, 548, 1043, 1048, 1166, 2356, 2625
NlaIV	GGNNCC	7 cut(s)	247, 681, 1704, 1848, 1849, 2087, 2164
NmuCI	GTSAC	6 cut(s)	614, 1145, 1771, 2034, 2515, 2711
NspI	RCATGY	2 cut(s)	1043, 2356
NspV	TTCGAA	1 cut(s)	1718
PacI	TTAATTAA	1 cut(s)	468
PagI	TCATGA	2 cut(s)	1044, 1162
PceI	AGGCCT	1 cut(s)	76
PciI	ACATGT	2 cut(s)	1039, 2352
PctI	GAATGC	1 cut(s)	532
PdmI	GAANNNNTTC	3 cut(s)	258, 2253, 2414
PfeI	GAWTC	8 cut(s)	176, 699, 782, 799, 1813, 2333, 2401, 2443
PkrI	GCNGC	9 cut(s)	154, 373, 451, 903, 1615, 2028, 2221, 2513, 2527
PleI	GAGTC	4 cut(s)	865, 1835, 2444, 2467
PmaCI	CACGTG	2 cut(s)	676, 1335
PmlI	CACGTG	2 cut(s)	676, 1335
PpsI	GAGTC	4 cut(s)	865, 1835, 2444, 2467
Ppu21I	YACGTR	2 cut(s)	676, 1335
PpuMI	RGGWCCY	2 cut(s)	1847, 2644
PscI	ACATGT	2 cut(s)	1039, 2352
PshAI	GACNNNNGTC	1 cut(s)	301
PshBI	ATTAAT	3 cut(s)	168, 468, 1200
PsiI	TTATAA	3 cut(s)	582, 1572, 2534
Psp5II	RGGWCCY	2 cut(s)	1847, 2644
Psp6I	CCWGG	3 cut(s)	689, 1339, 2646
PspCI	CACGTG	2 cut(s)	676, 1335
PspGI	CCWGG	3 cut(s)	689, 1339, 2646
PspN4I	GGNNCC	7 cut(s)	247, 681, 1704, 1848, 1849, 2087, 2164
PspPI	GGNCC	6 cut(s)	296, 653, 1557, 1847, 2198, 2644
PspPPI	RGGWCCY	2 cut(s)	1847, 2644
PstNI	CAGNNNCTG	2 cut(s)	2487, 2647
PsuI	RGATCY	3 cut(s)	245, 679, 1094
PvuII	CAGCTG	1 cut(s)	1616
RsaI	GTAC	4 cut(s)	354, 805, 2087, 2719
RsaNI	GTAC	4 cut(s)	353, 804, 2086, 2718
SatI	GCNGC	9 cut(s)	153, 372, 450, 902, 1614, 2027, 2220, 2512, 2526
Sau96I	GGNCC	6 cut(s)	296, 653, 1557, 1847, 2198, 2644
ScaI	AGTACT	1 cut(s)	2719
SchI	GAGTC	4 cut(s)	866, 1836, 2444, 2468
ScrFI	CCNGG	4 cut(s)	691, 1341, 1687, 2648
SduI	GDGCHC	2 cut(s)	1931, 2392
SfaNI	GCATC	5 cut(s)	211, 424, 1600, 1787, 1831
SfcI	CTRYAG	3 cut(s)	12, 774, 2079
SfuI	TTCGAA	1 cut(s)	1718
SinI	GGWCC	5 cut(s)	296, 1557, 1847, 2198, 2644
SmlI	CTYRAG	3 cut(s)	1817, 2614, 2721
SmoI	CTYRAG	3 cut(s)	1817, 2614, 2721
SseBI	AGGCCT	1 cut(s)	76
SsiI	CCGC	5 cut(s)	1365, 1931, 1939, 2191, 2307
SspI	AATATT	1 cut(s)	1204
SspMI	CTAG	5 cut(s)	885, 1067, 1691, 2139, 2159
StuI	AGGCCT	1 cut(s)	76
StyD4I	CCNGG	4 cut(s)	689, 1339, 1685, 2646
StyI	CCWWGG	2 cut(s)	144, 2138
TaiI	ACGT	2 cut(s)	678, 1337
TaqI	TCGA	6 cut(s)	230, 980, 1467, 1718, 1825, 2632
TaqII	GACCGA	1 cut(s)	1545
TatI	WGTACW	1 cut(s)	2717
TfiI	GAWTC	8 cut(s)	176, 699, 782, 799, 1813, 2333, 2401, 2443
TseFI	GTSAC	6 cut(s)	614, 1145, 1771, 2034, 2515, 2711
TseI	GCWGC	9 cut(s)	152, 371, 449, 901, 1613, 2026, 2219, 2511, 2525
Tsp45I	GTSAC	6 cut(s)	614, 1145, 1771, 2034, 2515, 2711
TspGWI	ACGGA	3 cut(s)	212, 238, 1516
VpaK11BI	GGWCC	5 cut(s)	296, 1557, 1847, 2198, 2644
VspI	ATTAAT	3 cut(s)	168, 468, 1200
XapI	RAATTY	4 cut(s)	226, 1059, 1154, 1433
XceI	RCATGY	2 cut(s)	1043, 2356
XmaJI	CCTAGG	1 cut(s)	2138
XmnI	GAANNNNTTC	3 cut(s)	258, 2253, 2414
XspI	CTAG	5 cut(s)	885, 1067, 1691, 2139, 2159
ZrmI	AGTACT	1 cut(s)	2719

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.