AT2G32740

macromolecule glycosylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012934
Physical Location & Seq
Forward (+)
13886794 .. 13888330
1537 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32740.1

Sequence Viewer

Length: 1407 bp
ATGGACAAATTCAATCCAAAAAAGGAAAAGACTGTGAAGAAACGAGCTCTAAAGGTTTTGACTGAAATCTCACCAACACCTTTGTTCTCCATGCTCTTTCTGCTTCACATCAGTCAAATTGCAACCTATCTCTCTCTTTCCGACAAAGAAACCCTAAATATCACTGTGAAAACTAAACAAGGTGGAACAGATACTTGTGCCGGAAGATACGTGTATATGCACAATCTTCCAAGCAGATTCAACGAAGATCTGATCAAGAGCTGTGAGGCATACATCGAATTACGAAACAAATGTAAGTACCTTATTAACTCTGGCTTCGGTCCACGTATCTTGGAGGAAGATCATAACCATACCACTCGAGTCTTAACCATCGAGACCGGTTCTTGGTACTACACAAACCAATTCATGCTTGAGGTTATCTTCCGAGAGAAAATGAGACACTACGAGTGTTTGACCAACGATTCTTCCCTTTCTTCCGTGGTTTTCGTCCCGTTTTATGCTGGATTCGATGTGAGACGCTTTTGGGGATACAACGTTAAGCTGAGAGACGAACTTGGTGAAGATCTAGCTCAATGGCTTAGGGAGAGACCAGAGTGGAGGAAAATGTATGGACGAGATCATTTCTTTGTCACGGGACGGGTTGGTAGAGATTTCAGGAGGGTTACAGATCAAGATTCGGATTGGGGAAACAAACTGATGCGGTTGCCTGAATTCGAGAACATAACTATGTTATCCATTGAGACAAATTCTAGGAGTAACGAGTTTGCGGTTCCTTATCCAACTTATTTCCATCCAAAGAGCCGAACCGAGGTTAAGAGATGGCAGAGGCAAGTGACGATGATGCAGAGACGATACTTGTTCTCGTTTGTCGGAGCTAATCGGCCAAAAATGGAGGAATCTATTAGAGGTGAGATTATAAGACAGTGCCTTGCATCACAAGGAAGATGCAAATTTCTTGATTGTGACACATCGAGTAAAGACTGTAGTGATCCGGTTAAGGTAGTGGAAGTTTTTCAAGATTCGGTTTTCTGTTTACAACCACCAGGAGATACACCTACTCGGAGATCAACATTTGATTCGATTTTAGCCGGTTGTATACCGGTTTTCTTTAGCGTTGATTCGGTCTATAACCAGTACAAGTGGTATTTTCCAAAAGATCGTACCAAGTACTCGGTTTACATCGCGGAGGAAGGTGTGAAAAAGGGGAAGGTTAGTATTGAGAAGTTATTGGCTAATGTTAGTGAAGAGAAGATTTCAAGGATGAGAAATGAGGTTGAGAAGATTATACCAAAGATAATTTATACAAAACCGGGGGAGGTTGGACCGGAAAAGATTGAAGATGCATTTGAAATTGCAGTGGCTAGGGTGCTTGAGAGAGTGTCATTGTTCAAGATGACACGGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

468

Amino Acids

54.84

Weight (kDa)

9.11

Isoelectric Point (pI)

53.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 66 - 406 4.4e-75 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012934)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G63450 AT2G31990 AT2G31990 AT2G31990 AT2G32740 AT5G41250
fragaria_vesca FvH4_5g02120
malus_domestica MD06G1081200.v1.1 MD14G1103100.v1.1
prunus_persica Prupe.5G098300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0198011
rosa_laevigata RLG00000003893
rosa_multiflora Rmu_co8320403.1_g000001 Rmu_sc0000221.1_g000033 Rmu_sc0001082.1_g000001
rosa_rugosa Rorug07G0045300
rosa_samantha Rh7CG178300
rosa_wichuraiana Rw7G014750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 917
AccI GTMKAC 1 cut(s) 1096
AccII CGCG 1 cut(s) 1184
AciI CCGC 3 cut(s) 700, 767, 1184
AclI AACGTT 1 cut(s) 534
AclWI GGATC 1 cut(s) 983
AcoI YGGCCR 1 cut(s) 881
AcsI RAATTY 4 cut(s) 8, 710, 745, 950
AfaI GTAC 5 cut(s) 299, 389, 1136, 1162, 1169
AfiI CCNNNNNNNGG 2 cut(s) 384, 808
AflIII ACRYGT 1 cut(s) 210
AgeI ACCGGT 2 cut(s) 377, 1099
AgsI TTSAA 7 cut(s) 13, 241, 1016, 1257, 1337, 1349, 1390
AjnI CCWGG 1 cut(s) 1042
AluBI AGCT 5 cut(s) 47, 261, 541, 569, 875
AluI AGCT 5 cut(s) 47, 261, 541, 569, 875
Alw21I GWGCWC 1 cut(s) 49
Alw26I GTCTC 7 cut(s) 368, 430, 508, 540, 580, 734, 841
AlwI GGATC 1 cut(s) 983
Ama87I CYCGRG 1 cut(s) 357
AoxI GGCC 1 cut(s) 881
ApoI RAATTY 4 cut(s) 8, 710, 745, 950
AsiGI ACCGGT 2 cut(s) 377, 1099
Asp700I GAANNNNTTC 1 cut(s) 1011
AspS9I GGNCC 2 cut(s) 320, 1322
AsuC2I CCSGG 1 cut(s) 1311
AsuHPI GGTGA 3 cut(s) 63, 569, 920
AvaI CYCGRG 1 cut(s) 357
AvaII GGWCC 2 cut(s) 320, 1322
BanII GRGCYC 1 cut(s) 49
Bbv12I GWGCWC 1 cut(s) 49
BccI CCATC 3 cut(s) 377, 798, 813
BciT130I CCWGG 1 cut(s) 1044
BciVI GTATCC 1 cut(s) 521
BclI TGATCA 1 cut(s) 252
BcnI CCSGG 1 cut(s) 1311
BcoDI GTCTC 7 cut(s) 368, 430, 508, 540, 580, 734, 841
BfaI CTAG 4 cut(s) 566, 750, 1362, 1405
BfmI CTRYAG 1 cut(s) 982
BfuI GTATCC 1 cut(s) 521
BglII AGATCT 2 cut(s) 247, 562
BmcAI AGTACT 1 cut(s) 1169
Bme1390I CCNGG 2 cut(s) 1044, 1311
Bme18I GGWCC 2 cut(s) 320, 1322
BmeT110I CYCGRG 1 cut(s) 357
BmgT120I GGNCC 2 cut(s) 320, 1322
BmiI GGNNCC 1 cut(s) 771
BmrFI CCNGG 2 cut(s) 1044, 1311
BmsI GCATC 5 cut(s) 687, 831, 935, 941, 1330
Bpu10I CCTNAGC 1 cut(s) 578
BpuEI CTTGAG 2 cut(s) 431, 1391
BpuMI CCSGG 1 cut(s) 1311
BsaAI YACGTR 2 cut(s) 211, 326
BsaI GGTCTC 2 cut(s) 368, 580
BsaJI CCNNGG 3 cut(s) 477, 807, 1310
BsaWI WCCGGW 4 cut(s) 377, 991, 1099, 1324
Bsc4I CCNNNNNNNGG 2 cut(s) 384, 808
Bse118I RCCGGY 3 cut(s) 377, 1088, 1099
Bse1I ACTGG 1 cut(s) 1132
BseBI CCWGG 1 cut(s) 1044
BseDI CCNNGG 3 cut(s) 477, 807, 1310
BseGI GGATG 2 cut(s) 790, 1266
BseLI CCNNNNNNNGG 2 cut(s) 384, 808
BseMII CTCAG 1 cut(s) 533
BseNI ACTGG 1 cut(s) 1132
Bsh1236I CGCG 1 cut(s) 1184
BshFI GGCC 1 cut(s) 883
BshTI ACCGGT 2 cut(s) 377, 1099
BsiHKAI GWGCWC 1 cut(s) 49
BsiHKCI CYCGRG 1 cut(s) 357
BsiSI CCGG 7 cut(s) 201, 378, 992, 1089, 1100, 1310, 1325
BslFI GGGAC 2 cut(s) 473, 648
BslI CCNNNNNNNGG 2 cut(s) 384, 808
BsmAI GTCTC 7 cut(s) 368, 430, 508, 540, 580, 734, 841
BsmBI CGTCTC 3 cut(s) 508, 540, 841
BsmFI GGGAC 2 cut(s) 473, 648
BsnI GGCC 1 cut(s) 883
Bso31I GGTCTC 2 cut(s) 368, 580
BsoBI CYCGRG 1 cut(s) 357
Bsp1286I GDGCHC 1 cut(s) 49
BspACI CCGC 3 cut(s) 700, 767, 1184
BspANI GGCC 1 cut(s) 883
BspCNI CTCAG 1 cut(s) 534
BspFNI CGCG 1 cut(s) 1184
BspLI GGNNCC 1 cut(s) 771
BspPI GGATC 1 cut(s) 983
BspTNI GGTCTC 2 cut(s) 368, 580
BsrFI RCCGGY 3 cut(s) 377, 1088, 1099
BsrI ACTGG 1 cut(s) 1132
BssAI RCCGGY 3 cut(s) 377, 1088, 1099
BssECI CCNNGG 3 cut(s) 477, 807, 1310
BssNAI GTATAC 1 cut(s) 1097
Bst1107I GTATAC 1 cut(s) 1097
Bst2UI CCWGG 1 cut(s) 1044
Bst4CI ACNGT 4 cut(s) 34, 166, 924, 983
Bst6I CTCTTC 1 cut(s) 1239
BstBAI YACGTR 2 cut(s) 211, 326
BstDEI CTNAG 2 cut(s) 542, 578
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 2 cut(s) 790, 1266
BstFNI CGCG 1 cut(s) 1184
BstMAI GTCTC 7 cut(s) 368, 430, 508, 540, 580, 734, 841
BstMWI GCNNNNNNNGC 1 cut(s) 100
BstNI CCWGG 1 cut(s) 1044
BstSCI CCNGG 2 cut(s) 1042, 1309
BstSFI CTRYAG 1 cut(s) 982
BstUI CGCG 1 cut(s) 1184
BstX2I RGATCY 3 cut(s) 247, 562, 1401
BstYI RGATCY 3 cut(s) 247, 562, 1401
BstZ17I GTATAC 1 cut(s) 1097
BsuI GTATCC 1 cut(s) 521
BsuRI GGCC 1 cut(s) 883
BtgI CCRYGG 1 cut(s) 477
BtgZI GCGATG 1 cut(s) 1165
BtsCI GGATG 2 cut(s) 790, 1266
BtsI GCAGTG 1 cut(s) 1362
BtsIMutI CAGTG 3 cut(s) 162, 929, 1362
Cfr10I RCCGGY 3 cut(s) 377, 1088, 1099
Cfr13I GGNCC 2 cut(s) 320, 1322
CseI GACGC 1 cut(s) 525
Csp6I GTAC 5 cut(s) 298, 388, 1135, 1161, 1168
CspAI ACCGGT 2 cut(s) 377, 1099
CspCI CAANNNNNGTGG 2 cut(s) 312, 347
CviAII CATG 2 cut(s) 91, 406
CviQI GTAC 5 cut(s) 298, 388, 1135, 1161, 1168
DdeI CTNAG 2 cut(s) 542, 578
EaeI YGGCCR 1 cut(s) 881
Eam1104I CTCTTC 1 cut(s) 1239
EarI CTCTTC 1 cut(s) 1239
Ecl136II GAGCTC 1 cut(s) 47
Eco24I GRGCYC 1 cut(s) 49
Eco31I GGTCTC 2 cut(s) 368, 580
Eco47I GGWCC 2 cut(s) 320, 1322
Eco53kI GAGCTC 1 cut(s) 47
Eco88I CYCGRG 1 cut(s) 357
EcoICRI GAGCTC 1 cut(s) 47
EcoRI GAATTC 1 cut(s) 710
EcoRII CCWGG 1 cut(s) 1042
EcoT22I ATGCAT 1 cut(s) 1345
EcoT38I GRGCYC 1 cut(s) 49
Esp3I CGTCTC 3 cut(s) 508, 540, 841
FaeI CATG 2 cut(s) 94, 409
FaqI GGGAC 2 cut(s) 473, 648
FatI CATG 2 cut(s) 90, 405
FbaI TGATCA 1 cut(s) 252
FblI GTMKAC 1 cut(s) 1096
FokI GGATG 2 cut(s) 777, 1273
FriOI GRGCYC 1 cut(s) 49
FspBI CTAG 4 cut(s) 566, 750, 1362, 1405
HaeIII GGCC 1 cut(s) 883
HapII CCGG 7 cut(s) 201, 378, 992, 1089, 1100, 1310, 1325
HgaI GACGC 1 cut(s) 525
Hin1II CATG 2 cut(s) 94, 409
HinfI GANTC 9 cut(s) 237, 360, 461, 504, 674, 896, 1019, 1076, 1118
HpaII CCGG 7 cut(s) 201, 378, 992, 1089, 1100, 1310, 1325
HphI GGTGA 3 cut(s) 63, 569, 920
Hpy166II GTNNAC 4 cut(s) 323, 1034, 1097, 1177
Hpy188I TCNGA 6 cut(s) 142, 252, 425, 679, 872, 1062
Hpy188III TCNNGA 8 cut(s) 256, 373, 655, 671, 715, 956, 1016, 1390
Hpy8I GTNNAC 4 cut(s) 323, 1034, 1097, 1177
HpyAV CCTTC 2 cut(s) 1184, 1201
HpyCH4III ACNGT 4 cut(s) 34, 166, 924, 983
HpyCH4IV ACGT 3 cut(s) 210, 325, 534
HpyCH4V TGCA 7 cut(s) 122, 220, 844, 932, 948, 1343, 1355
HpyF10VI GCNNNNNNNGC 1 cut(s) 100
HpyF3I CTNAG 2 cut(s) 542, 578
HpySE526I ACGT 3 cut(s) 210, 325, 534
Hsp92II CATG 2 cut(s) 94, 409
Ksp22I TGATCA 1 cut(s) 252
LmnI GCTCC 1 cut(s) 872
LweI GCATC 5 cut(s) 687, 831, 935, 941, 1330
MaeI CTAG 4 cut(s) 566, 750, 1362, 1405
MaeII ACGT 3 cut(s) 210, 325, 534
MaeIII GTNAC 5 cut(s) 628, 661, 755, 832, 962
MflI RGATCY 3 cut(s) 247, 562, 1401
MhlI GDGCHC 1 cut(s) 49
MluCI AATT 9 cut(s) 8, 117, 278, 401, 710, 745, 950, 1296, 1350
MlyI GAGTC 1 cut(s) 369
MmeI TCCRAC 4 cut(s) 165, 803, 850, 1300
Mph1103I ATGCAT 1 cut(s) 1345
MroXI GAANNNNTTC 1 cut(s) 1011
MseI TTAA 5 cut(s) 306, 365, 537, 813, 996
MslI CAYNNNNRTG 1 cut(s) 725
MspI CCGG 7 cut(s) 201, 378, 992, 1089, 1100, 1310, 1325
MspR9I CCNGG 2 cut(s) 1044, 1311
MvaI CCWGG 1 cut(s) 1044
MvnI CGCG 1 cut(s) 1184
MwoI GCNNNNNNNGC 1 cut(s) 100
NciI CCSGG 1 cut(s) 1311
NlaIII CATG 2 cut(s) 94, 409
NlaIV GGNNCC 1 cut(s) 771
NmuCI GTSAC 3 cut(s) 628, 832, 962
NsiI ATGCAT 1 cut(s) 1345
PaeR7I CTCGAG 1 cut(s) 357
PdmI GAANNNNTTC 1 cut(s) 1011
PfeI GAWTC 8 cut(s) 237, 461, 504, 674, 896, 1019, 1076, 1118
PinAI ACCGGT 2 cut(s) 377, 1099
PleI GAGTC 1 cut(s) 368
PpsI GAGTC 1 cut(s) 368
Ppu21I YACGTR 2 cut(s) 211, 326
PsiI TTATAA 1 cut(s) 917
Psp124BI GAGCTC 1 cut(s) 49
Psp1406I AACGTT 1 cut(s) 534
Psp6I CCWGG 1 cut(s) 1042
PspGI CCWGG 1 cut(s) 1042
PspN4I GGNNCC 1 cut(s) 771
PspPI GGNCC 2 cut(s) 320, 1322
PspXI VCTCGAGB 1 cut(s) 357
PsuI RGATCY 3 cut(s) 247, 562, 1401
RsaI GTAC 5 cut(s) 299, 389, 1136, 1162, 1169
RsaNI GTAC 5 cut(s) 298, 388, 1135, 1161, 1168
RseI CAYNNNNRTG 1 cut(s) 725
SacI GAGCTC 1 cut(s) 49
SaqAI TTAA 5 cut(s) 306, 365, 537, 813, 996
Sau96I GGNCC 2 cut(s) 320, 1322
ScaI AGTACT 1 cut(s) 1169
SchI GAGTC 1 cut(s) 369
ScrFI CCNGG 2 cut(s) 1044, 1311
SduI GDGCHC 1 cut(s) 49
SfaNI GCATC 5 cut(s) 687, 831, 935, 941, 1330
SfcI CTRYAG 1 cut(s) 982
Sfr274I CTCGAG 1 cut(s) 357
SinI GGWCC 2 cut(s) 320, 1322
SlaI CTCGAG 1 cut(s) 357
SmiMI CAYNNNNRTG 1 cut(s) 725
SmlI CTYRAG 3 cut(s) 357, 410, 1370
SmoI CTYRAG 3 cut(s) 357, 410, 1370
Sse9I AATT 9 cut(s) 8, 117, 278, 401, 710, 745, 950, 1296, 1350
SsiI CCGC 3 cut(s) 700, 767, 1184
SspMI CTAG 4 cut(s) 566, 750, 1362, 1405
SstI GAGCTC 1 cut(s) 49
StyD4I CCNGG 2 cut(s) 1042, 1309
TaaI ACNGT 4 cut(s) 34, 166, 924, 983
TaiI ACGT 3 cut(s) 213, 328, 537
TaqI TCGA 7 cut(s) 276, 358, 372, 507, 714, 971, 1079
TaqII GACCGA 2 cut(s) 308, 1111
TasI AATT 9 cut(s) 8, 117, 278, 401, 710, 745, 950, 1296, 1350
TatI WGTACW 2 cut(s) 1134, 1167
TfiI GAWTC 8 cut(s) 237, 461, 504, 674, 896, 1019, 1076, 1118
Tru1I TTAA 5 cut(s) 306, 365, 537, 813, 996
Tru9I TTAA 5 cut(s) 306, 365, 537, 813, 996
TscAI CASTG 3 cut(s) 169, 929, 1362
TseFI GTSAC 3 cut(s) 628, 832, 962
Tsp45I GTSAC 3 cut(s) 628, 832, 962
TspDTI ATGAA 1 cut(s) 394
TspGWI ACGGA 1 cut(s) 466
TspRI CASTG 3 cut(s) 169, 929, 1362
VpaK11BI GGWCC 2 cut(s) 320, 1322
XapI RAATTY 4 cut(s) 8, 710, 745, 950
XhoI CTCGAG 1 cut(s) 357
XmiI GTMKAC 1 cut(s) 1096
XmnI GAANNNNTTC 1 cut(s) 1011
XspI CTAG 4 cut(s) 566, 750, 1362, 1405
ZrmI AGTACT 1 cut(s) 1169
Zsp2I ATGCAT 1 cut(s) 1345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.