Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0012934

Physical Location & Seq

Genomic Coordinates Forward (+)

19928491 .. 19930224

Gene Structure 1734 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1081200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1734 bp

ATGGTCGGTGTTTCCATGTCGAAACGAAAACCAAAACTCCCCATGCAACCGGCCCCGAAAGAATCCTGTAGCTGTTTCCTTTTTCTTTTCAAATTTCTCTTTCGAGTCCCGGTTGCATTTTTCATTCTCCTTATCTACCTCTGGTCCTCCTCCGCCACCATTTTCTCCGGCAACATTGTCCACATTTGTGTTTCCTCCCGGAAGCTCAACGACCATTACTGCCTTTCTGCTGGTAAAACCCAACCCAAGTTTGAAATCCCAATTCCGATCGTCAATCATAGTTCCATTTATCTCTACACTCTTGACAAGAGTTTGAACCTACCTCTTCCCTCATCGCCTCCTTTGTACCGCGGAAATGGTTCCACTAGTCCTCAATCCACTGTCAATGGTGAGAAGGTGAGAGAGACCGCTGAAGTTATCCGAGATGAGTCTGAACCGGTAACTATGAGGAATGGCGAGAACAAAGACGGAGAAGAGCAGGTTGCGAATGCAAAGAAGGACGTTGAAGAACAGATGCGACTGCACAGATCATGGATTTCGGATAAGAAGCAAGCCGGGTGCGAAGGGAGGGGAGTATATGTGTATGACTTGCCATCCAAGTTTAATTTTGACTTGATTGGTCAGTGCAGAGACATGGTTCCATGGATGGACTTCTGCAAGTACTTCAAAAATGAAGCCATGGGAGAACCAATTCCAAAATTCGGAAACGGTTGGTACCAGACACATCAGTACTCATTGGAGCCGATTTTTCATCAGAGGGTTATGAAGCATCCTTGCAGGGTTTATAATGAGGATGAAGCCAAGCTTTTCTACATTCCATTTTACGGCGGTTTGGATATTCTGAGATGGCATTTCAAGAATGTCTCCAGCGACGTGAAAGACACATTGAGTTTGGAGCTCATAACATGGCTTGAGAAACAAAGATCATGGACTAAGAATTCTCGCAAGGATCATGTCATTGTGTTGGGAAAAATTTCATGGGATTTTCGGAGAAAGGACCGCTCTTGGGGAACCAGATTCTTGGAGATTGATCAAATGAAAAACCCGGTAAAGCTTTTGATTGAGAGGCAGCCATGGCAGCCAAATGACATAGGAATCCCACACCCAACATCCTTCCATCCCAGCTCAGACGACGACATCATGGCATGGCAATGGAAAATTTTAAGCTCGCACCGAAAACAGTTAGTGGGCTTTGCTGGTGCAGAAAGGCCTGGTCAAGCTGAGAGCATAAGGTCTATGCTAATCAAACAGTGCAATCCATCCTCCAAAAATGGAAACCAATGCCGATTTCTTGACTGTGGTTCGGGCGGATGTGATCAGCCAGAGACAGTCATTGACCTCTTCCTAGAGTCTGAATTCTGCCTGCAGCCCCCAGGGGACAGCCCTACTAGAAAATCACTGTTTGATTCGCTGATAGCGGGCTGCATTCCCGTGCTTTTTGATCCTTTCACAGCTTATTACCAGTACCCGTGGCATTTGCCGGAGGATCATGGAAAGTATTCTGTGTTTGTGGATCAAGAGGAAGTGAGGGGAATGAAGGTGAATGTGGTGGAGATGTTGATGAAGATTTCAAAGGAGAAGAGGGATGATATGAGGAGGTTTATAGTGTATGAATTGTTGCCTGGTTTGGTGTATGGGGATTCAAATGCAAAATTTGAAAAGTTTGAGGATGCATTTTCCATCACAATGAGTAATCTCATGGAGAGGGTGACCAAATTGAAATTAAATGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005975 GO:0005976 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016049 GO:0016051 GO:0016740 GO:0016757 GO:0021700 GO:0022622 GO:0030154 GO:0032501 GO:0032502 GO:0040007 GO:0042546 GO:0043170 GO:0044085 GO:0044238 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0060560 GO:0071554 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090627 GO:0099402 GO:1901576 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Exostosin

Protein Analysis

578

Amino Acids

66.61

Weight (kDa)

8.37

Isoelectric Point (pI)

44.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exostosin_GT47	PF03016	187 - 511	8.6e-77	Exostosin GT47 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012934)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63450 AT2G31990 AT2G31990 AT2G31990 AT2G32740 AT5G41250
fragaria_vesca	FvH4_5g02120
malus_domestica	MD06G1081200.v1.1 MD14G1103100.v1.1
prunus_persica	Prupe.5G098300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0198011
rosa_laevigata	RLG00000003893
rosa_multiflora	Rmu_co8320403.1_g000001 Rmu_sc0000221.1_g000033 Rmu_sc0001082.1_g000001
rosa_rugosa	Rorug07G0045300
rosa_samantha	Rh7CG178300
rosa_wichuraiana	Rw7G014750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	786
Acc36I	ACCTGC	1 cut(s)	469
Acc65I	GGTACC	1 cut(s)	714
AccB1I	GGYRCC	1 cut(s)	714
AccBSI	CCGCTC	1 cut(s)	1002
AccII	CGCG	1 cut(s)	351
AciI	CCGC	8 cut(s)	153, 349, 351, 408, 828, 1000, 1308, 1418
AclWI	GGATC	4 cut(s)	957, 1436, 1494, 1521
AcsI	RAATTY	7 cut(s)	92, 698, 937, 972, 1158, 1355, 1652
AcuI	CTGAAG	1 cut(s)	432
AfaI	GTAC	5 cut(s)	347, 662, 716, 731, 1466
AfiI	CCNNNNNNNGG	3 cut(s)	701, 824, 1006
AgeI	ACCGGT	1 cut(s)	436
AhlI	ACTAGT	1 cut(s)	365
AjiI	CACGTC	1 cut(s)	874
AjnI	CCWGG	3 cut(s)	1210, 1372, 1621
AjuI	GAANNNNNNNTTGG	2 cut(s)	1272, 1304
AleI	CACNNNNGTG	1 cut(s)	186
AluBI	AGCT	9 cut(s)	72, 205, 805, 898, 1054, 1125, 1167, 1220, 1454
AluI	AGCT	9 cut(s)	72, 205, 805, 898, 1054, 1125, 1167, 1220, 1454
Alw21I	GWGCWC	1 cut(s)	900
Alw26I	GTCTC	4 cut(s)	398, 624, 868, 1319
AlwI	GGATC	4 cut(s)	957, 1436, 1494, 1521
AoxI	GGCC	2 cut(s)	51, 1208
ApeKI	GCWGC	4 cut(s)	1069, 1078, 1366, 1422
ApoI	RAATTY	7 cut(s)	92, 698, 937, 972, 1158, 1355, 1652
AsiGI	ACCGGT	1 cut(s)	436
Asp700I	GAANNNNTTC	4 cut(s)	358, 690, 973, 1498
Asp718I	GGTACC	1 cut(s)	714
AspS9I	GGNCC	3 cut(s)	52, 144, 997
AsuC2I	CCSGG	4 cut(s)	110, 199, 556, 1046
AsuHPI	GGTGA	4 cut(s)	401, 409, 1552, 1720
AvaII	GGWCC	2 cut(s)	144, 997
BanI	GGYRCC	1 cut(s)	714
BanII	GRGCYC	1 cut(s)	900
Bbv12I	GWGCWC	1 cut(s)	900
BbvI	GCAGC	4 cut(s)	1081, 1090, 1378, 1409
BccI	CCATC	6 cut(s)	601, 640, 840, 1125, 1267, 1688
BceAI	ACGGC	1 cut(s)	841
BciT130I	CCWGG	3 cut(s)	1212, 1374, 1623
BclI	TGATCA	2 cut(s)	1030, 1315
BcnI	CCSGG	4 cut(s)	110, 199, 556, 1046
BcoDI	GTCTC	4 cut(s)	398, 624, 868, 1319
BcuI	ACTAGT	1 cut(s)	365
BfaI	CTAG	3 cut(s)	366, 1346, 1389
BfmI	CTRYAG	2 cut(s)	67, 1364
BfuAI	ACCTGC	1 cut(s)	469
BisI	GCNGC	4 cut(s)	1070, 1079, 1367, 1423
BlsI	GCNGC	4 cut(s)	1071, 1080, 1368, 1424
BmcAI	AGTACT	2 cut(s)	662, 731
Bme1390I	CCNGG	7 cut(s)	110, 199, 556, 1046, 1212, 1374, 1623
Bme18I	GGWCC	2 cut(s)	144, 997
BmgBI	CACGTC	1 cut(s)	874
BmgT120I	GGNCC	3 cut(s)	52, 144, 997
BmiI	GGNNCC	6 cut(s)	54, 361, 639, 716, 741, 1012
BmrFI	CCNGG	7 cut(s)	110, 199, 556, 1046, 1212, 1374, 1623
BmsI	GCATC	3 cut(s)	504, 778, 1660
BpmI	CTGGAG	1 cut(s)	850
BpuEI	CTTGAG	1 cut(s)	932
BpuMI	CCSGG	4 cut(s)	110, 199, 556, 1046
BsaI	GGTCTC	1 cut(s)	398
BsaJI	CCNNGG	7 cut(s)	349, 641, 678, 1073, 1372, 1373, 1469
BsaWI	WCCGGW	1 cut(s)	436
BsaXI	ACNNNNNCTCC	4 cut(s)	21, 51, 564, 594
Bsc4I	CCNNNNNNNGG	3 cut(s)	701, 824, 1006
Bse118I	RCCGGY	2 cut(s)	49, 436
Bse1I	ACTGG	1 cut(s)	1462
Bse3DI	GCAATG	1 cut(s)	1157
BseBI	CCWGG	3 cut(s)	1212, 1374, 1623
BseDI	CCNNGG	7 cut(s)	349, 641, 678, 1073, 1372, 1373, 1469
BseLI	CCNNNNNNNGG	3 cut(s)	701, 824, 1006
BseMI	GCAATG	1 cut(s)	1157
BseMII	CTCAG	3 cut(s)	833, 1140, 1212
BseNI	ACTGG	1 cut(s)	1462
BseRI	GAGGAG	2 cut(s)	139, 1609
BseXI	GCAGC	4 cut(s)	1081, 1090, 1378, 1409
BseYI	CCCAGC	1 cut(s)	1121
BsgI	GTGCAG	3 cut(s)	506, 646, 1221
Bsh1236I	CGCG	1 cut(s)	351
Bsh1285I	CGRYCG	1 cut(s)	270
BshFI	GGCC	2 cut(s)	53, 1210
BshNI	GGYRCC	1 cut(s)	714
BshTI	ACCGGT	1 cut(s)	436
BsiEI	CGRYCG	1 cut(s)	270
BsiHKAI	GWGCWC	1 cut(s)	900
BsiSI	CCGG	8 cut(s)	50, 110, 168, 199, 437, 555, 1046, 1481
BslFI	GGGAC	2 cut(s)	92, 1391
BslI	CCNNNNNNNGG	3 cut(s)	701, 824, 1006
BsmAI	GTCTC	4 cut(s)	398, 624, 868, 1319
BsmFI	GGGAC	2 cut(s)	92, 1391
BsmI	GAATGC	2 cut(s)	493, 1425
BsnI	GGCC	2 cut(s)	53, 1210
Bso31I	GGTCTC	1 cut(s)	398
Bsp1286I	GDGCHC	1 cut(s)	900
Bsp143I	GATC	9 cut(s)	267, 527, 923, 949, 1030, 1315, 1441, 1486, 1513
Bsp19I	CCATGG	3 cut(s)	641, 678, 1073
BspACI	CCGC	8 cut(s)	153, 349, 351, 408, 828, 1000, 1308, 1418
BspANI	GGCC	2 cut(s)	53, 1210
BspCNI	CTCAG	3 cut(s)	834, 1139, 1213
BspFNI	CGCG	1 cut(s)	351
BspLI	GGNNCC	6 cut(s)	54, 361, 639, 716, 741, 1012
BspMAI	CTGCAG	1 cut(s)	1368
BspMI	ACCTGC	1 cut(s)	469
BspPI	GGATC	4 cut(s)	957, 1436, 1494, 1521
BspQI	GCTCTTC	1 cut(s)	468
BspT107I	GGYRCC	1 cut(s)	714
BspTNI	GGTCTC	1 cut(s)	398
BsrBI	CCGCTC	1 cut(s)	1002
BsrDI	GCAATG	1 cut(s)	1157
BsrFI	RCCGGY	2 cut(s)	49, 436
BsrI	ACTGG	1 cut(s)	1462
BssAI	RCCGGY	2 cut(s)	49, 436
BssECI	CCNNGG	7 cut(s)	349, 641, 678, 1073, 1372, 1373, 1469
BssMI	GATC	9 cut(s)	267, 527, 923, 949, 1030, 1315, 1441, 1486, 1513
BssT1I	CCWWGG	3 cut(s)	641, 678, 1073
Bst2UI	CCWGG	3 cut(s)	1212, 1374, 1623
Bst4CI	ACNGT	7 cut(s)	382, 710, 1182, 1251, 1298, 1330, 1401
Bst6I	CTCTTC	4 cut(s)	330, 468, 1346, 1574
BstC8I	GCNNGC	4 cut(s)	552, 1169, 1364, 1420
BstDEI	CTNAG	4 cut(s)	842, 933, 1126, 1221
BstDSI	CCRYGG	5 cut(s)	349, 641, 678, 1073, 1469
BstEII	GGTNACC	1 cut(s)	1708
BstFNI	CGCG	1 cut(s)	351
BstKTI	GATC	9 cut(s)	270, 530, 926, 952, 1033, 1318, 1444, 1489, 1516
BstMAI	GTCTC	4 cut(s)	398, 624, 868, 1319
BstMBI	GATC	9 cut(s)	267, 527, 923, 949, 1030, 1315, 1441, 1486, 1513
BstMCI	CGRYCG	1 cut(s)	270
BstMWI	GCNNNNNNNGC	2 cut(s)	1075, 1078
BstNI	CCWGG	3 cut(s)	1212, 1374, 1623
BstPI	GGTNACC	1 cut(s)	1708
BstSCI	CCNGG	7 cut(s)	108, 197, 554, 1044, 1210, 1372, 1621
BstSFI	CTRYAG	2 cut(s)	67, 1364
BstUI	CGCG	1 cut(s)	351
BstV1I	GCAGC	4 cut(s)	1081, 1090, 1378, 1409
BstXI	CCANNNNNNTGG	1 cut(s)	1021
BsuRI	GGCC	2 cut(s)	53, 1210
BtgI	CCRYGG	5 cut(s)	349, 641, 678, 1073, 1469
BtgZI	GCGATG	1 cut(s)	318
BtrI	CACGTC	1 cut(s)	874
BtsIMutI	CAGTG	4 cut(s)	378, 629, 1256, 1397
BveI	ACCTGC	1 cut(s)	469
Cac8I	GCNNGC	4 cut(s)	552, 1169, 1364, 1420
Cfr10I	RCCGGY	2 cut(s)	49, 436
Cfr13I	GGNCC	3 cut(s)	52, 144, 997
Cfr42I	CCGCGG	1 cut(s)	352
Csp6I	GTAC	5 cut(s)	346, 661, 715, 730, 1465
CspAI	ACCGGT	1 cut(s)	436
CviQI	GTAC	5 cut(s)	346, 661, 715, 730, 1465
DdeI	CTNAG	4 cut(s)	842, 933, 1126, 1221
DpnI	GATC	9 cut(s)	269, 529, 925, 951, 1032, 1317, 1443, 1488, 1515
DpnII	GATC	9 cut(s)	267, 527, 923, 949, 1030, 1315, 1441, 1486, 1513
Eam1104I	CTCTTC	4 cut(s)	330, 468, 1346, 1574
EarI	CTCTTC	4 cut(s)	330, 468, 1346, 1574
EciI	GGCGGA	2 cut(s)	142, 1323
Ecl136II	GAGCTC	1 cut(s)	898
Eco130I	CCWWGG	3 cut(s)	641, 678, 1073
Eco147I	AGGCCT	1 cut(s)	1210
Eco24I	GRGCYC	1 cut(s)	900
Eco31I	GGTCTC	1 cut(s)	398
Eco47I	GGWCC	2 cut(s)	144, 997
Eco53kI	GAGCTC	1 cut(s)	898
Eco57I	CTGAAG	1 cut(s)	432
Eco91I	GGTNACC	1 cut(s)	1708
EcoICRI	GAGCTC	1 cut(s)	898
EcoO65I	GGTNACC	1 cut(s)	1708
EcoRI	GAATTC	2 cut(s)	937, 1355
EcoRII	CCWGG	3 cut(s)	1210, 1372, 1621
EcoT14I	CCWWGG	3 cut(s)	641, 678, 1073
EcoT22I	ATGCAT	1 cut(s)	1675
EcoT38I	GRGCYC	1 cut(s)	900
ErhI	CCWWGG	3 cut(s)	641, 678, 1073
FalI	AAGNNNNNCTT	2 cut(s)	789, 821
FaqI	GGGAC	2 cut(s)	92, 1391
FauI	CCCGC	1 cut(s)	1411
FbaI	TGATCA	2 cut(s)	1030, 1315
Fnu4HI	GCNGC	4 cut(s)	1070, 1079, 1367, 1423
FriOI	GRGCYC	1 cut(s)	900
Fsp4HI	GCNGC	4 cut(s)	1070, 1079, 1367, 1423
FspBI	CTAG	3 cut(s)	366, 1346, 1389
GluI	GCNGC	4 cut(s)	1070, 1079, 1367, 1423
GsaI	CCCAGC	1 cut(s)	1125
GsuI	CTGGAG	1 cut(s)	850
HaeIII	GGCC	2 cut(s)	53, 1210
HapII	CCGG	8 cut(s)	50, 110, 168, 199, 437, 555, 1046, 1481
HindIII	AAGCTT	2 cut(s)	803, 1052
HinfI	GANTC	8 cut(s)	62, 105, 428, 1017, 1095, 1349, 1406, 1640
HpaII	CCGG	8 cut(s)	50, 110, 168, 199, 437, 555, 1046, 1481
HphI	GGTGA	4 cut(s)	401, 409, 1552, 1720
Hpy166II	GTNNAC	1 cut(s)	181
Hpy188III	TCNNGA	4 cut(s)	302, 856, 1292, 1517
Hpy8I	GTNNAC	1 cut(s)	181
Hpy99I	CGWCG	2 cut(s)	875, 1136
HpyAV	CCTTC	5 cut(s)	388, 490, 557, 1123, 1531
HpyCH4III	ACNGT	7 cut(s)	382, 710, 1182, 1251, 1298, 1330, 1401
HpyCH4IV	ACGT	2 cut(s)	501, 873
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1075, 1078
HpyF3I	CTNAG	4 cut(s)	842, 933, 1126, 1221
HpySE526I	ACGT	2 cut(s)	501, 873
KpnI	GGTACC	1 cut(s)	718
Ksp22I	TGATCA	2 cut(s)	1030, 1315
KspI	CCGCGG	1 cut(s)	352
Kzo9I	GATC	9 cut(s)	267, 527, 923, 949, 1030, 1315, 1441, 1486, 1513
LguI	GCTCTTC	1 cut(s)	468
LmnI	GCTCC	2 cut(s)	739, 895
Lsp1109I	GCAGC	4 cut(s)	1081, 1090, 1378, 1409
LweI	GCATC	3 cut(s)	504, 778, 1660
MaeI	CTAG	3 cut(s)	366, 1346, 1389
MaeII	ACGT	2 cut(s)	501, 873
MaeIII	GTNAC	2 cut(s)	439, 1708
MalI	GATC	9 cut(s)	269, 529, 925, 951, 1032, 1317, 1443, 1488, 1515
MbiI	CCGCTC	1 cut(s)	1002
MboI	GATC	9 cut(s)	267, 527, 923, 949, 1030, 1315, 1441, 1486, 1513
MboII	GAAGA	6 cut(s)	317, 485, 518, 1333, 1576, 1591
MhlI	GDGCHC	1 cut(s)	900
MlyI	GAGTC	3 cut(s)	114, 437, 1358
Mph1103I	ATGCAT	1 cut(s)	1675
MroXI	GAANNNNTTC	4 cut(s)	358, 690, 973, 1498
MseI	TTAA	3 cut(s)	603, 1163, 1724
MslI	CAYNNNNRTG	4 cut(s)	186, 1150, 1430, 1685
MspA1I	CMGCKG	2 cut(s)	351, 410
MspI	CCGG	8 cut(s)	50, 110, 168, 199, 437, 555, 1046, 1481
MspR9I	CCNGG	7 cut(s)	110, 199, 556, 1046, 1212, 1374, 1623
Mva1269I	GAATGC	2 cut(s)	493, 1425
MvaI	CCWGG	3 cut(s)	1212, 1374, 1623
MvnI	CGCG	1 cut(s)	351
MwoI	GCNNNNNNNGC	2 cut(s)	1075, 1078
NciI	CCSGG	4 cut(s)	110, 199, 556, 1046
NcoI	CCATGG	3 cut(s)	641, 678, 1073
NdeII	GATC	9 cut(s)	267, 527, 923, 949, 1030, 1315, 1441, 1486, 1513
NlaIV	GGNNCC	6 cut(s)	54, 361, 639, 716, 741, 1012
NmuCI	GTSAC	1 cut(s)	1708
NsiI	ATGCAT	1 cut(s)	1675
OliI	CACNNNNGTG	1 cut(s)	186
PasI	CCCWGGG	1 cut(s)	1373
PceI	AGGCCT	1 cut(s)	1210
PciSI	GCTCTTC	1 cut(s)	468
PctI	GAATGC	2 cut(s)	493, 1425
PdmI	GAANNNNTTC	4 cut(s)	358, 690, 973, 1498
PfeI	GAWTC	5 cut(s)	62, 1017, 1095, 1406, 1640
PfoI	TCCNGGA	1 cut(s)	197
PinAI	ACCGGT	1 cut(s)	436
PkrI	GCNGC	4 cut(s)	1071, 1080, 1368, 1424
Ple19I	CGATCG	1 cut(s)	270
PleI	GAGTC	3 cut(s)	113, 436, 1357
PpsI	GAGTC	3 cut(s)	113, 436, 1357
PsiI	TTATAA	1 cut(s)	786
Psp124BI	GAGCTC	1 cut(s)	900
Psp6I	CCWGG	3 cut(s)	1210, 1372, 1621
PspEI	GGTNACC	1 cut(s)	1708
PspFI	CCCAGC	1 cut(s)	1121
PspGI	CCWGG	3 cut(s)	1210, 1372, 1621
PspN4I	GGNNCC	6 cut(s)	54, 361, 639, 716, 741, 1012
PspPI	GGNCC	3 cut(s)	52, 144, 997
PstI	CTGCAG	1 cut(s)	1368
PvuI	CGATCG	1 cut(s)	270
RsaI	GTAC	5 cut(s)	347, 662, 716, 731, 1466
RsaNI	GTAC	5 cut(s)	346, 661, 715, 730, 1465
RseI	CAYNNNNRTG	4 cut(s)	186, 1150, 1430, 1685
SacI	GAGCTC	1 cut(s)	900
SacII	CCGCGG	1 cut(s)	352
SapI	GCTCTTC	1 cut(s)	468
SaqAI	TTAA	3 cut(s)	603, 1163, 1724
SatI	GCNGC	4 cut(s)	1070, 1079, 1367, 1423
Sau3AI	GATC	9 cut(s)	267, 527, 923, 949, 1030, 1315, 1441, 1486, 1513
Sau96I	GGNCC	3 cut(s)	52, 144, 997
ScaI	AGTACT	2 cut(s)	662, 731
SchI	GAGTC	3 cut(s)	114, 437, 1358
ScrFI	CCNGG	7 cut(s)	110, 199, 556, 1046, 1212, 1374, 1623
SduI	GDGCHC	1 cut(s)	900
SfaNI	GCATC	3 cut(s)	504, 778, 1660
SfcI	CTRYAG	2 cut(s)	67, 1364
Sfr303I	CCGCGG	1 cut(s)	352
SgrBI	CCGCGG	1 cut(s)	352
SinI	GGWCC	2 cut(s)	144, 997
SmiMI	CAYNNNNRTG	4 cut(s)	186, 1150, 1430, 1685
SmlI	CTYRAG	1 cut(s)	911
SmoI	CTYRAG	1 cut(s)	911
SpeI	ACTAGT	1 cut(s)	365
SseBI	AGGCCT	1 cut(s)	1210
SsiI	CCGC	8 cut(s)	153, 349, 351, 408, 828, 1000, 1308, 1418
SspMI	CTAG	3 cut(s)	366, 1346, 1389
SstI	GAGCTC	1 cut(s)	900
StuI	AGGCCT	1 cut(s)	1210
StyD4I	CCNGG	7 cut(s)	108, 197, 554, 1044, 1210, 1372, 1621
StyI	CCWWGG	3 cut(s)	641, 678, 1073
TaaI	ACNGT	7 cut(s)	382, 710, 1182, 1251, 1298, 1330, 1401
TaiI	ACGT	2 cut(s)	504, 876
TaqI	TCGA	2 cut(s)	20, 103
TatI	WGTACW	2 cut(s)	660, 729
TfiI	GAWTC	5 cut(s)	62, 1017, 1095, 1406, 1640
Tru1I	TTAA	3 cut(s)	603, 1163, 1724
Tru9I	TTAA	3 cut(s)	603, 1163, 1724
TscAI	CASTG	4 cut(s)	385, 629, 1256, 1404
TseFI	GTSAC	1 cut(s)	1708
TseI	GCWGC	4 cut(s)	1069, 1078, 1366, 1422
Tsp45I	GTSAC	1 cut(s)	1708
TspGWI	ACGGA	1 cut(s)	483
TspRI	CASTG	4 cut(s)	385, 629, 1256, 1404
VpaK11BI	GGWCC	2 cut(s)	144, 997
XapI	RAATTY	7 cut(s)	92, 698, 937, 972, 1158, 1355, 1652
XmnI	GAANNNNTTC	4 cut(s)	358, 690, 973, 1498
XspI	CTAG	3 cut(s)	366, 1346, 1389
ZrmI	AGTACT	2 cut(s)	662, 731
Zsp2I	ATGCAT	1 cut(s)	1675

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.