Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0014581

Physical Location & Seq

Genomic Coordinates Reverse (-)

13962213 .. 13965531

Gene Structure 3319 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G32920.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1323 bp

ATGTATAAATCACCATTAACGTTGCTCACACTTCTTACTATTTGTTTTGGATTCTTCGATCTCAGCAGTGCTCTTTATGGATCTTCGTCACCTGTGGTTCAGCTCACTGCTTCTAACTTTAAGTCCAAGGTTCTTAATTCAAATGGAGTTGTTTTGGTAGAGTTCTTTGCACCATGGTGTGGTCATTGCAAAGCTCTAACACCTACATGGGAGAAAGTGGCTAATATTCTGAAAGGAGTTGCTACTGTGGCTGCAATTGATGCAGATGCTCATCAGTCTGCCGCGCAGGATTATGGTATTAAAGGTTTCCCAACAATTAAGGTTTTTGTTCCTGGAAAGGCTCCAATCGATTACCAAGGAGCAAGAGATGCCAAATCCATTGCTAATTTTGCTTATAAGCAGATCAAGGGTCTTTTGAGTGACCGTTTGGAAGGCAAAAGTAAACCAACTGGAGGAGGATCTAAAGAAAAGAAATCTGAACCTAGTGCATCTGTGGAACTGAACGCTAGCAATTTTGATGATTTGGTCATCGAAAGCAATGAACTTTGGATCGTTGAGTTTTTTGCACCTTGGTGTGGACATTGCAAAAAGCTTGCCCCGGAGTGGAAGAGGGCTGCAAAAAATTTGCAGGGAAAGGTGAAATTAGGTCATGTCAATTGTGATGTTGAGCAGTCGATAATGAGCAGATTCAAAGTTCAAGGATTCCCTACAATTTTGGTATTTGGTCCAGACAAAAGTAGTCCATATCCTTACGAGGGTGCTAGATCTGCTTCAGCGATAGAATCTTTTGCTTCTGAACTTGTGGAATCCAGTGCTGGCCCTGTTGAAGTGACTGAACTAACTGGACCGGATGTCATGGAAAAGAAGTGTGGTTCTGCTGCTATTTGCTTTATCTCTTTCTTACCTGACATTCTTGACTCCAAAGCTGAAGGAAGGAATAAGTACCTCGAGATGTTATTATCAGTTGCAGAGAAGTTTAAGAAGCAGCCTTACAGTTTTATGTGGGTGGCGGCTGTAACTCAAATGGATTTGGAGAAGCGAGTTAATGTAGGAGGATACGGTTATCCAGCAATGGTAGCCATGAATGTGAAGAAAGGAGTATATGCTCCTCTTAAAAGCGCCTTTGAGCTTCAACACCTCTTAGAATTTGTGAAAGATGCTGGAACCGGTGGAAAAGGAAATGTGCCAATGAATGGAACACCAGAGATCGTGAAGACAAAGGAGTGGGATGGTAAAGATGGAGAGTTGATTGAGGAAGATGAATTCTCACTTGATGAGCTCATGGGAGGTGATGATGCTGTTGGTTCTAAGGATGAGTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003756 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005788 GO:0005794 GO:0005886 GO:0006457 GO:0006950 GO:0008150 GO:0009505 GO:0009987 GO:0012505 GO:0016020 GO:0016853 GO:0016860 GO:0016864 GO:0030312 GO:0031974 GO:0033554 GO:0034976 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051716 GO:0070013 GO:0071944 GO:0140096

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Thioredoxin

Protein Analysis

440

Amino Acids

47.75

Weight (kDa)

5.63

Isoelectric Point (pI)

32.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thioredoxin	PF00085	32 - 131	4.3e-31	Thioredoxin
Thioredoxin	PF00085	165 - 264	4.2e-28	Thioredoxin
Thioredoxin_6	PF13848	198 - 386	6.7e-07	Thioredoxin-like domain
Thioredox_PDIA6_C	PF24541	274 - 386	4.2e-08	PDIA6-like, C-terminal thioredoxin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014581)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04980 AT2G32920
fragaria_vesca	FvH4_6g41940
malus_domestica	MD09G1116100.v1.1 MD17G1107800.v1.1
prunus_persica	Prupe.3G208600_v2.0.a1
pyrus_communis	pycom09g04050
rosa_chinensis	RchiOBHm_Chr2g0157821
rosa_laevigata	RLG00000021023
rosa_multiflora	Rmu_sc0002441.1_g000014
rosa_roxburghii	Rroxscaffold_2G00091920
rosa_rugosa	Rorug02G0464000
rosa_samantha	Rh2AG529300 Rh2BG543400 Rh2CG513600 Rh2DG552200
rosa_wichuraiana	Rw2G043760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	396
AccB7I	CCANNNNNTGG	2 cut(s)	179, 1193
AccII	CGCG	1 cut(s)	284
AciI	CCGC	2 cut(s)	282, 1010
AclI	AACGTT	1 cut(s)	20
AclWI	GGATC	3 cut(s)	88, 466, 557
AcsI	RAATTY	3 cut(s)	622, 1145, 1262
AcuI	CTGAAG	2 cut(s)	756, 948
AfaI	GTAC	1 cut(s)	944
AfiI	CCNNNNNNNGG	6 cut(s)	179, 452, 575, 603, 755, 1193
AgeI	ACCGGT	1 cut(s)	1166
AgsI	TTSAA	5 cut(s)	141, 691, 698, 827, 1133
AhdI	GACNNNNNGTC	1 cut(s)	851
AjnI	CCWGG	1 cut(s)	331
AleI	CACNNNNGTG	2 cut(s)	175, 571
AluBI	AGCT	6 cut(s)	103, 194, 592, 926, 1129, 1279
AluI	AGCT	6 cut(s)	103, 194, 592, 926, 1129, 1279
Alw21I	GWGCWC	2 cut(s)	73, 1281
AlwI	GGATC	3 cut(s)	88, 466, 557
Ama87I	CYCGRG	1 cut(s)	947
AoxI	GGCC	1 cut(s)	817
ApeKI	GCWGC	4 cut(s)	251, 614, 878, 985
ApoI	RAATTY	3 cut(s)	622, 1145, 1262
AsiGI	ACCGGT	1 cut(s)	1166
AspLEI	GCGC	2 cut(s)	286, 1121
AspS9I	GGNCC	3 cut(s)	725, 818, 845
AsuC2I	CCSGG	1 cut(s)	599
AsuHPI	GGTGA	4 cut(s)	3, 81, 649, 1301
AsuNHI	GCTAGC	1 cut(s)	506
AvaI	CYCGRG	1 cut(s)	947
AvaII	GGWCC	2 cut(s)	725, 845
BanII	GRGCYC	1 cut(s)	1281
BarI	GAAGNNNNNNTAC	2 cut(s)	974, 1006
BbsI	GAAGAC	1 cut(s)	1220
Bbv12I	GWGCWC	2 cut(s)	73, 1281
BbvI	GCAGC	4 cut(s)	238, 601, 865, 997
BccI	CCATC	2 cut(s)	1223, 1232
BciT130I	CCWGG	1 cut(s)	333
BciVI	GTATCC	1 cut(s)	1049
BcnI	CCSGG	1 cut(s)	599
BfaI	CTAG	3 cut(s)	483, 507, 762
BfoI	RGCGCY	1 cut(s)	1122
BfuI	GTATCC	1 cut(s)	1049
BglII	AGATCT	1 cut(s)	764
BisI	GCNGC	6 cut(s)	252, 282, 615, 879, 986, 1011
BlsI	GCNGC	6 cut(s)	253, 283, 616, 880, 987, 1012
Bme1390I	CCNGG	2 cut(s)	333, 599
Bme18I	GGWCC	2 cut(s)	725, 845
BmeRI	GACNNNNNGTC	1 cut(s)	851
BmeT110I	CYCGRG	1 cut(s)	947
BmgT120I	GGNCC	3 cut(s)	725, 818, 845
BmiI	GGNNCC	2 cut(s)	342, 1165
BmrFI	CCNGG	2 cut(s)	333, 599
BmsI	GCATC	6 cut(s)	250, 256, 358, 497, 1147, 1285
BmtI	GCTAGC	1 cut(s)	510
BpiI	GAAGAC	1 cut(s)	1220
BpmI	CTGGAG	1 cut(s)	471
BpuMI	CCSGG	1 cut(s)	599
Bsa29I	ATCGAT	1 cut(s)	348
BsaBI	GATNNNNATC	1 cut(s)	270
BsaJI	CCNNGG	5 cut(s)	126, 173, 355, 569, 597
BsaWI	WCCGGW	2 cut(s)	847, 1166
Bsc4I	CCNNNNNNNGG	6 cut(s)	179, 452, 575, 603, 755, 1193
Bse118I	RCCGGY	1 cut(s)	1166
Bse1I	ACTGG	3 cut(s)	454, 810, 847
Bse3DI	GCAATG	5 cut(s)	184, 378, 544, 580, 1077
Bse8I	GATNNNNATC	1 cut(s)	270
BseBI	CCWGG	1 cut(s)	333
BseCI	ATCGAT	1 cut(s)	348
BseDI	CCNNGG	5 cut(s)	126, 173, 355, 569, 597
BseGI	GGATG	3 cut(s)	856, 1234, 1318
BseJI	GATNNNNATC	1 cut(s)	270
BseLI	CCNNNNNNNGG	6 cut(s)	179, 452, 575, 603, 755, 1193
BseMI	GCAATG	5 cut(s)	184, 378, 544, 580, 1077
BseMII	CTCAG	1 cut(s)	76
BseNI	ACTGG	3 cut(s)	454, 810, 847
BseRI	GAGGAG	2 cut(s)	468, 1098
BseXI	GCAGC	4 cut(s)	238, 601, 865, 997
Bsh1236I	CGCG	1 cut(s)	284
BshFI	GGCC	1 cut(s)	819
BshTI	ACCGGT	1 cut(s)	1166
BshVI	ATCGAT	1 cut(s)	348
BsiHKAI	GWGCWC	2 cut(s)	73, 1281
BsiHKCI	CYCGRG	1 cut(s)	947
BsiSI	CCGG	3 cut(s)	599, 848, 1167
BslI	CCNNNNNNNGG	6 cut(s)	179, 452, 575, 603, 755, 1193
BsnI	GGCC	1 cut(s)	819
BsoBI	CYCGRG	1 cut(s)	947
Bsp1286I	GDGCHC	2 cut(s)	73, 1281
Bsp143I	GATC	7 cut(s)	58, 80, 402, 458, 549, 764, 1206
Bsp19I	CCATGG	1 cut(s)	173
BspACI	CCGC	2 cut(s)	282, 1010
BspANI	GGCC	1 cut(s)	819
BspCNI	CTCAG	1 cut(s)	75
BspDI	ATCGAT	1 cut(s)	348
BspFNI	CGCG	1 cut(s)	284
BspLI	GGNNCC	2 cut(s)	342, 1165
BspOI	GCTAGC	1 cut(s)	510
BspPI	GGATC	3 cut(s)	88, 466, 557
BsrDI	GCAATG	5 cut(s)	184, 378, 544, 580, 1077
BsrFI	RCCGGY	1 cut(s)	1166
BsrI	ACTGG	3 cut(s)	454, 810, 847
BssAI	RCCGGY	1 cut(s)	1166
BssECI	CCNNGG	5 cut(s)	126, 173, 355, 569, 597
BssMI	GATC	7 cut(s)	58, 80, 402, 458, 549, 764, 1206
BssT1I	CCWWGG	4 cut(s)	126, 173, 355, 569
Bst2UI	CCWGG	1 cut(s)	333
Bst4CI	ACNGT	4 cut(s)	247, 425, 995, 1061
Bst6I	CTCTTC	1 cut(s)	602
BstAPI	GCANNNNNTGC	2 cut(s)	260, 368
BstC8I	GCNNGC	3 cut(s)	508, 594, 817
BstDEI	CTNAG	3 cut(s)	62, 1141, 1308
BstDSI	CCRYGG	1 cut(s)	173
BstF5I	GGATG	3 cut(s)	856, 1234, 1318
BstFNI	CGCG	1 cut(s)	284
BstH2I	RGCGCY	1 cut(s)	1122
BstHHI	GCGC	2 cut(s)	286, 1121
BstKTI	GATC	7 cut(s)	61, 83, 405, 461, 552, 767, 1209
BstMBI	GATC	7 cut(s)	58, 80, 402, 458, 549, 764, 1206
BstMWI	GCNNNNNNNGC	6 cut(s)	248, 260, 368, 389, 767, 1076
BstNI	CCWGG	1 cut(s)	333
BstSCI	CCNGG	2 cut(s)	331, 597
BstUI	CGCG	1 cut(s)	284
BstV1I	GCAGC	4 cut(s)	238, 601, 865, 997
BstV2I	GAAGAC	1 cut(s)	1220
BstX2I	RGATCY	3 cut(s)	80, 458, 764
BstYI	RGATCY	3 cut(s)	80, 458, 764
Bsu15I	ATCGAT	1 cut(s)	348
BsuI	GTATCC	1 cut(s)	1049
BsuRI	GGCC	1 cut(s)	819
BsuTUI	ATCGAT	1 cut(s)	348
BtgI	CCRYGG	1 cut(s)	173
BtsCI	GGATG	3 cut(s)	856, 1234, 1318
BtsI	GCAGTG	2 cut(s)	73, 105
BtsIMutI	CAGTG	3 cut(s)	73, 105, 817
Cac8I	GCNNGC	3 cut(s)	508, 594, 817
CfoI	GCGC	2 cut(s)	286, 1121
Cfr10I	RCCGGY	1 cut(s)	1166
Cfr13I	GGNCC	3 cut(s)	725, 818, 845
ClaI	ATCGAT	1 cut(s)	348
Csp6I	GTAC	1 cut(s)	943
CspAI	ACCGGT	1 cut(s)	1166
CviAII	CATG	6 cut(s)	174, 207, 650, 856, 1081, 1282
CviQI	GTAC	1 cut(s)	943
DdeI	CTNAG	3 cut(s)	62, 1141, 1308
DpnI	GATC	7 cut(s)	60, 82, 404, 460, 551, 766, 1208
DpnII	GATC	7 cut(s)	58, 80, 402, 458, 549, 764, 1206
DriI	GACNNNNNGTC	1 cut(s)	851
Eam1104I	CTCTTC	1 cut(s)	602
Eam1105I	GACNNNNNGTC	1 cut(s)	851
EarI	CTCTTC	1 cut(s)	602
Ecl136II	GAGCTC	1 cut(s)	1279
Eco130I	CCWWGG	4 cut(s)	126, 173, 355, 569
Eco24I	GRGCYC	1 cut(s)	1281
Eco47I	GGWCC	2 cut(s)	725, 845
Eco53kI	GAGCTC	1 cut(s)	1279
Eco57I	CTGAAG	2 cut(s)	756, 948
Eco88I	CYCGRG	1 cut(s)	947
EcoICRI	GAGCTC	1 cut(s)	1279
EcoRI	GAATTC	1 cut(s)	1262
EcoRII	CCWGG	1 cut(s)	331
EcoT14I	CCWWGG	4 cut(s)	126, 173, 355, 569
EcoT38I	GRGCYC	1 cut(s)	1281
ErhI	CCWWGG	4 cut(s)	126, 173, 355, 569
FaeI	CATG	6 cut(s)	177, 210, 653, 859, 1084, 1285
FatI	CATG	6 cut(s)	173, 206, 649, 855, 1080, 1281
Fnu4HI	GCNGC	6 cut(s)	252, 282, 615, 879, 986, 1011
FokI	GGATG	2 cut(s)	863, 1241
FriOI	GRGCYC	1 cut(s)	1281
Fsp4HI	GCNGC	6 cut(s)	252, 282, 615, 879, 986, 1011
FspBI	CTAG	3 cut(s)	483, 507, 762
GlaI	GCGC	2 cut(s)	285, 1120
GluI	GCNGC	6 cut(s)	252, 282, 615, 879, 986, 1011
GsuI	CTGGAG	1 cut(s)	471
HaeII	RGCGCY	1 cut(s)	1122
HaeIII	GGCC	1 cut(s)	819
HapII	CCGG	3 cut(s)	599, 848, 1167
HhaI	GCGC	2 cut(s)	286, 1121
Hin1II	CATG	6 cut(s)	177, 210, 653, 859, 1084, 1285
Hin6I	GCGC	2 cut(s)	284, 1119
HinP1I	GCGC	2 cut(s)	284, 1119
HindIII	AAGCTT	1 cut(s)	590
HinfI	GANTC	6 cut(s)	51, 687, 702, 782, 806, 917
HpaII	CCGG	3 cut(s)	599, 848, 1167
HphI	GGTGA	4 cut(s)	3, 81, 649, 1301
Hpy166II	GTNNAC	2 cut(s)	443, 578
Hpy188I	TCNGA	3 cut(s)	231, 478, 796
Hpy188III	TCNNGA	4 cut(s)	728, 914, 949, 1210
Hpy8I	GTNNAC	2 cut(s)	443, 578
HpyAV	CCTTC	3 cut(s)	425, 923, 927
HpyCH4III	ACNGT	4 cut(s)	247, 425, 995, 1061
HpyCH4IV	ACGT	1 cut(s)	20
HpyF10VI	GCNNNNNNNGC	6 cut(s)	248, 260, 368, 389, 767, 1076
HpyF3I	CTNAG	3 cut(s)	62, 1141, 1308
HpySE526I	ACGT	1 cut(s)	20
Hsp92II	CATG	6 cut(s)	177, 210, 653, 859, 1084, 1285
HspAI	GCGC	2 cut(s)	284, 1119
Kzo9I	GATC	7 cut(s)	58, 80, 402, 458, 549, 764, 1206
LmnI	GCTCC	3 cut(s)	346, 359, 1111
Lsp1109I	GCAGC	4 cut(s)	238, 601, 865, 997
LweI	GCATC	6 cut(s)	250, 256, 358, 497, 1147, 1285
MaeI	CTAG	3 cut(s)	483, 507, 762
MaeII	ACGT	1 cut(s)	20
MaeIII	GTNAC	4 cut(s)	87, 419, 829, 1015
MalI	GATC	7 cut(s)	60, 82, 404, 460, 551, 766, 1208
MboI	GATC	7 cut(s)	58, 80, 402, 458, 549, 764, 1206
MboII	GAAGA	6 cut(s)	46, 75, 619, 1102, 1225, 1268
MfeI	CAATTG	2 cut(s)	255, 655
MflI	RGATCY	3 cut(s)	80, 458, 764
MhlI	GDGCHC	2 cut(s)	73, 1281
MlyI	GAGTC	1 cut(s)	911
MseI	TTAA	8 cut(s)	17, 120, 135, 300, 318, 978, 1044, 1113
MslI	CAYNNNNRTG	4 cut(s)	175, 205, 571, 1085
MspI	CCGG	3 cut(s)	599, 848, 1167
MspR9I	CCNGG	2 cut(s)	333, 599
MunI	CAATTG	2 cut(s)	255, 655
MvaI	CCWGG	1 cut(s)	333
MvnI	CGCG	1 cut(s)	284
MwoI	GCNNNNNNNGC	6 cut(s)	248, 260, 368, 389, 767, 1076
NciI	CCSGG	1 cut(s)	599
NcoI	CCATGG	1 cut(s)	173
NdeII	GATC	7 cut(s)	58, 80, 402, 458, 549, 764, 1206
NheI	GCTAGC	1 cut(s)	506
NlaIII	CATG	6 cut(s)	177, 210, 653, 859, 1084, 1285
NlaIV	GGNNCC	2 cut(s)	342, 1165
NmuCI	GTSAC	3 cut(s)	87, 419, 829
OliI	CACNNNNGTG	2 cut(s)	175, 571
PaeR7I	CTCGAG	1 cut(s)	947
PfeI	GAWTC	5 cut(s)	51, 687, 702, 782, 806
PflMI	CCANNNNNTGG	2 cut(s)	179, 1193
PfoI	TCCNGGA	1 cut(s)	331
PinAI	ACCGGT	1 cut(s)	1166
PkrI	GCNGC	6 cut(s)	253, 283, 616, 880, 987, 1012
PleI	GAGTC	1 cut(s)	911
PpsI	GAGTC	1 cut(s)	911
PsiI	TTATAA	1 cut(s)	396
Psp124BI	GAGCTC	1 cut(s)	1281
Psp1406I	AACGTT	1 cut(s)	20
Psp6I	CCWGG	1 cut(s)	331
PspGI	CCWGG	1 cut(s)	331
PspN4I	GGNNCC	2 cut(s)	342, 1165
PspPI	GGNCC	3 cut(s)	725, 818, 845
PsuI	RGATCY	3 cut(s)	80, 458, 764
RsaI	GTAC	1 cut(s)	944
RsaNI	GTAC	1 cut(s)	943
RseI	CAYNNNNRTG	4 cut(s)	175, 205, 571, 1085
SacI	GAGCTC	1 cut(s)	1281
SaqAI	TTAA	8 cut(s)	17, 120, 135, 300, 318, 978, 1044, 1113
SatI	GCNGC	6 cut(s)	252, 282, 615, 879, 986, 1011
Sau3AI	GATC	7 cut(s)	58, 80, 402, 458, 549, 764, 1206
Sau96I	GGNCC	3 cut(s)	725, 818, 845
SchI	GAGTC	1 cut(s)	911
ScrFI	CCNGG	2 cut(s)	333, 599
SduI	GDGCHC	2 cut(s)	73, 1281
SfaNI	GCATC	6 cut(s)	250, 256, 358, 497, 1147, 1285
Sfr274I	CTCGAG	1 cut(s)	947
SinI	GGWCC	2 cut(s)	725, 845
SlaI	CTCGAG	1 cut(s)	947
SmiMI	CAYNNNNRTG	4 cut(s)	175, 205, 571, 1085
SmlI	CTYRAG	1 cut(s)	947
SmoI	CTYRAG	1 cut(s)	947
SsiI	CCGC	2 cut(s)	282, 1010
SspI	AATATT	1 cut(s)	226
SspMI	CTAG	3 cut(s)	483, 507, 762
SstI	GAGCTC	1 cut(s)	1281
StyD4I	CCNGG	2 cut(s)	331, 597
StyI	CCWWGG	4 cut(s)	126, 173, 355, 569
TaaI	ACNGT	4 cut(s)	247, 425, 995, 1061
TaiI	ACGT	1 cut(s)	23
TaqI	TCGA	5 cut(s)	57, 348, 531, 674, 948
TauI	GCSGC	2 cut(s)	284, 1013
TfiI	GAWTC	5 cut(s)	51, 687, 702, 782, 806
Tru1I	TTAA	8 cut(s)	17, 120, 135, 300, 318, 978, 1044, 1113
Tru9I	TTAA	8 cut(s)	17, 120, 135, 300, 318, 978, 1044, 1113
TscAI	CASTG	3 cut(s)	73, 112, 817
TseFI	GTSAC	3 cut(s)	87, 419, 829
TseI	GCWGC	4 cut(s)	251, 614, 878, 985
Tsp45I	GTSAC	3 cut(s)	87, 419, 829
TspDTI	ATGAA	4 cut(s)	555, 1097, 1205, 1275
TspRI	CASTG	3 cut(s)	73, 112, 817
Van91I	CCANNNNNTGG	2 cut(s)	179, 1193
VpaK11BI	GGWCC	2 cut(s)	725, 845
XapI	RAATTY	3 cut(s)	622, 1145, 1262
XhoI	CTCGAG	1 cut(s)	947
XspI	CTAG	3 cut(s)	483, 507, 762

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.