RLG00000021023

Belongs to the protein disulfide isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014581
Physical Location & Seq
Reverse (-)
71155613 .. 71158769
3157 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021023

Sequence Viewer

Length: 1203 bp
ATGATCACCAAGTTTGATTCTTATGAAGTTCTGGACTCGAACCGAGTTGTTCTGGTTGAATTCTTTGCGCCGTGGTGTGGACATTGTCAAGCTCTGACTCCTATATGGGAGAAAGCGGCTACTGTGTTGAAGGGTGTGGCCACCGTAGCTGCCCTTGATGCTGATGCGCACAAGTCCCTTGCTCAGGAATATGGTATTAGAGGATTCCCGACAATAAAGGTGTTCGTACCTGGACAGCCTCCAGTTGATTATCAGGGAGCGAGGGAAGTGAAACCCATTGCAGAATTTGCAGTCAAACAGATTAAGGCACTATTGAAGGACCGCTTAAGTGGAAAAGCAACTGGAGGATCAAGTGAAAAACCTAAACCTAATGCTTCAGTAGAACTAAATTCCCAGAATTTTGATGAATTAGTTGTTAAAAGCAAAGATCTCTGGGTTGTGGAGTTTTTTGCACCTTGGTGTGGGCACTGTAAAAAATTGGCACCTGAGTGGAAGAAGGCTGCCAAAAACTTGAAGGGAAAGGTGAAGTTGGGACATGTTGACTGTGATGCAGAGAAGTCTCTTATGAGCAGGTTCAATGTTCAAGGATTCCCAACAATATTGGTATTTGGAGCTGACAAAGATAGCCCAGTACCTTATGAGGGTGCGAGGACTGCTTCAGCAATTGAATCATTTTCTCTCGAACAACTAGAAACAAATGTTGCACCTCCTGAAGTGACTGAGCTAACTGGCCCTGATGTATTGGAAGAGAAATGTGGTTCGGCTGCCATCTGTTTTGTGGCTTTTCTACCTGATATCTTGGATTCCAAGGCAGAAGGGAGGAACAAATACCTTCAGCAGTTATTATCTGTTGCAGAGAAGTTCAAGAAGAGTCCTTATAGCTATGTCTGGGCAGCAGCTGGTAAGCAGCAAGACCTCGAGAATCGCGTAGGTGTTGGTGGTTATGGATATCCAGCATTGGTTGCCTTAAATGTGAAAAAAGGTGCTTATGCCCCACTTAAGAGTGCCTTCGAGCTTGATCAGATCATCGAATTTGTGAGAGAAGCTGGGCGTGGTGGAAAGGGAAACTTGCCTCTGAACGGCACCCCTAACATTGTGAAGAGCGAACCATGGGATGGTAAAGACGGAGAGATCATTGAAGAGGATGAGTTCTCCCTTGACGAACTGATGGGGGAAGATACTAAGAGCAAGGATGAATTGTGA

Protein Analysis

401

Amino Acids

43.71

Weight (kDa)

5.36

Isoelectric Point (pI)

30.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 9 - 96 1.8e-26 Thioredoxin
OST3_OST6 PF04756 124 - 210 9.4e-06 OST3 / OST6 family, transporter family
Thioredoxin PF00085 127 - 225 1e-30 Thioredoxin
Thioredoxin_2 PF13098 143 - 216 2.3e-06 Thioredoxin-like domain
Thioredoxin_6 PF13848 160 - 347 2.1e-10 Thioredoxin-like domain
Thioredox_PDIA6_C PF24541 233 - 347 7.4e-09 PDIA6-like, C-terminal thioredoxin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014581)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04980 AT2G32920
fragaria_vesca FvH4_6g41940
malus_domestica MD09G1116100.v1.1 MD17G1107800.v1.1
prunus_persica Prupe.3G208600_v2.0.a1
pyrus_communis pycom09g04050
rosa_chinensis RchiOBHm_Chr2g0157821
rosa_laevigata RLG00000021023
rosa_multiflora Rmu_sc0002441.1_g000014
rosa_roxburghii Rroxscaffold_2G00091920
rosa_rugosa Rorug02G0464000
rosa_samantha Rh2AG529300 Rh2BG543400 Rh2CG513600 Rh2DG552200
rosa_wichuraiana Rw2G043760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 168
Acc36I ACCTGC 1 cut(s) 561
AccB1I GGYRCC 2 cut(s) 481, 1082
AccB7I CCANNNNNTGG 1 cut(s) 1115
AccII CGCG 1 cut(s) 927
AciI CCGC 2 cut(s) 116, 322
AclWI GGATC 1 cut(s) 355
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 5 cut(s) 59, 284, 388, 397, 1031
AcuI CTGAAG 4 cut(s) 360, 642, 732, 818
AfaI GTAC 2 cut(s) 228, 633
AfiI CCNNNNNNNGG 6 cut(s) 77, 184, 461, 641, 1079, 1115
AflII CTTAAG 2 cut(s) 325, 998
AflIII ACRYGT 1 cut(s) 535
AgsI TTSAA 9 cut(s) 59, 130, 316, 514, 577, 584, 668, 865, 1139
AjnI CCWGG 1 cut(s) 229
AleI CACNNNNGTG 2 cut(s) 457, 487
AluBI AGCT 8 cut(s) 92, 149, 614, 724, 882, 899, 1015, 1046
AluI AGCT 8 cut(s) 92, 149, 614, 724, 882, 899, 1015, 1046
Alw26I GTCTC 1 cut(s) 564
AlwI GGATC 1 cut(s) 355
AlwNI CAGNNNCTG 1 cut(s) 899
Ama87I CYCGRG 1 cut(s) 917
AoxI GGCC 2 cut(s) 138, 730
ApeKI GCWGC 6 cut(s) 149, 500, 764, 893, 896, 907
ApoI RAATTY 5 cut(s) 59, 284, 388, 397, 1031
AspLEI GCGC 2 cut(s) 70, 169
AspS9I GGNCC 2 cut(s) 319, 731
AsuHPI GGTGA 1 cut(s) 535
AvaI CYCGRG 1 cut(s) 917
AvaII GGWCC 1 cut(s) 319
BaeGI GKGCMC 1 cut(s) 468
BalI TGGCCA 1 cut(s) 140
BanI GGYRCC 2 cut(s) 481, 1082
BbvI GCAGC 6 cut(s) 136, 487, 751, 905, 908, 919
BccI CCATC 3 cut(s) 776, 1109, 1162
BceAI ACGGC 2 cut(s) 55, 1096
BciT130I CCWGG 1 cut(s) 231
BclI TGATCA 2 cut(s) 3, 1018
BcoDI GTCTC 1 cut(s) 564
BfaI CTAG 1 cut(s) 689
BfrI CTTAAG 2 cut(s) 325, 998
BfuAI ACCTGC 1 cut(s) 561
BglII AGATCT 1 cut(s) 427
BisI GCNGC 7 cut(s) 117, 150, 501, 765, 894, 897, 908
BlsI GCNGC 7 cut(s) 118, 151, 502, 766, 895, 898, 909
Bme1390I CCNGG 1 cut(s) 231
Bme18I GGWCC 1 cut(s) 319
BmeT110I CYCGRG 1 cut(s) 917
BmgT120I GGNCC 2 cut(s) 319, 731
BmiI GGNNCC 2 cut(s) 483, 1084
BmrFI CCNGG 1 cut(s) 231
BmrI ACTGGG 1 cut(s) 623
BmsI GCATC 3 cut(s) 148, 154, 538
BmuI ACTGGG 1 cut(s) 623
BpmI CTGGAG 2 cut(s) 225, 363
Bpu10I CCTNAGC 1 cut(s) 183
BsaJI CCNNGG 4 cut(s) 71, 455, 807, 1109
Bsc4I CCNNNNNNNGG 6 cut(s) 77, 184, 461, 641, 1079, 1115
Bse1I ACTGG 4 cut(s) 242, 346, 629, 733
Bse3DI GCAATG 1 cut(s) 276
BseBI CCWGG 1 cut(s) 231
BseDI CCNNGG 4 cut(s) 71, 455, 807, 1109
BseGI GGATG 3 cut(s) 1120, 1150, 1198
BseLI CCNNNNNNNGG 6 cut(s) 77, 184, 461, 641, 1079, 1115
BseMI GCAATG 1 cut(s) 276
BseMII CTCAG 3 cut(s) 197, 477, 711
BseNI ACTGG 4 cut(s) 242, 346, 629, 733
BseSI GKGCMC 1 cut(s) 468
BseXI GCAGC 6 cut(s) 136, 487, 751, 905, 908, 919
BseYI CCCAGC 1 cut(s) 1046
Bsh1236I CGCG 1 cut(s) 927
BshFI GGCC 2 cut(s) 140, 732
BshNI GGYRCC 2 cut(s) 481, 1082
BsiHKCI CYCGRG 1 cut(s) 917
BslFI GGGAC 2 cut(s) 160, 546
BslI CCNNNNNNNGG 6 cut(s) 77, 184, 461, 641, 1079, 1115
BsmAI GTCTC 1 cut(s) 564
BsmFI GGGAC 2 cut(s) 160, 546
BsnI GGCC 2 cut(s) 140, 732
BsoBI CYCGRG 1 cut(s) 917
Bsp1286I GDGCHC 1 cut(s) 468
Bsp143I GATC 6 cut(s) 3, 347, 427, 1018, 1023, 1131
Bsp19I CCATGG 1 cut(s) 1109
BspACI CCGC 2 cut(s) 116, 322
BspANI GGCC 2 cut(s) 140, 732
BspCNI CTCAG 3 cut(s) 196, 478, 712
BspFNI CGCG 1 cut(s) 927
BspLI GGNNCC 2 cut(s) 483, 1084
BspMI ACCTGC 1 cut(s) 561
BspPI GGATC 1 cut(s) 355
BspQI GCTCTTC 1 cut(s) 1094
BspT107I GGYRCC 2 cut(s) 481, 1082
BspTI CTTAAG 2 cut(s) 325, 998
BsrDI GCAATG 1 cut(s) 276
BsrI ACTGG 4 cut(s) 242, 346, 629, 733
BssECI CCNNGG 4 cut(s) 71, 455, 807, 1109
BssMI GATC 6 cut(s) 3, 347, 427, 1018, 1023, 1131
BssT1I CCWWGG 3 cut(s) 455, 807, 1109
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 4 cut(s) 124, 145, 470, 545
Bst6I CTCTTC 4 cut(s) 741, 863, 1094, 1134
BstAFI CTTAAG 2 cut(s) 325, 998
BstAPI GCANNNNNTGC 2 cut(s) 287, 962
BstDEI CTNAG 4 cut(s) 183, 486, 720, 1182
BstDSI CCRYGG 2 cut(s) 71, 1109
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 3 cut(s) 1120, 1150, 1198
BstFNI CGCG 1 cut(s) 927
BstHHI GCGC 2 cut(s) 70, 169
BstKTI GATC 6 cut(s) 6, 350, 430, 1021, 1026, 1134
BstMAI GTCTC 1 cut(s) 564
BstMBI GATC 6 cut(s) 3, 347, 427, 1018, 1023, 1131
BstMWI GCNNNNNNNGC 5 cut(s) 146, 158, 287, 653, 962
BstNI CCWGG 1 cut(s) 231
BstNSI RCATGY 1 cut(s) 539
BstSCI CCNGG 1 cut(s) 229
BstSLI GKGCMC 1 cut(s) 468
BstUI CGCG 1 cut(s) 927
BstV1I GCAGC 6 cut(s) 136, 487, 751, 905, 908, 919
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
BsuRI GGCC 2 cut(s) 140, 732
BtgI CCRYGG 2 cut(s) 71, 1109
BtsCI GGATG 3 cut(s) 1120, 1150, 1198
BtsIMutI CAGTG 1 cut(s) 466
BveI ACCTGC 1 cut(s) 561
CaiI CAGNNNCTG 1 cut(s) 899
CfoI GCGC 2 cut(s) 70, 169
Cfr13I GGNCC 2 cut(s) 319, 731
Csp6I GTAC 2 cut(s) 227, 632
CviAII CATG 2 cut(s) 536, 1110
CviQI GTAC 2 cut(s) 227, 632
DdeI CTNAG 4 cut(s) 183, 486, 720, 1182
DpnI GATC 6 cut(s) 5, 349, 429, 1020, 1025, 1133
DpnII GATC 6 cut(s) 3, 347, 427, 1018, 1023, 1131
EaeI YGGCCR 1 cut(s) 138
Eam1104I CTCTTC 4 cut(s) 741, 863, 1094, 1134
EarI CTCTTC 4 cut(s) 741, 863, 1094, 1134
Eco130I CCWWGG 3 cut(s) 455, 807, 1109
Eco32I GATATC 2 cut(s) 796, 950
Eco47I GGWCC 1 cut(s) 319
Eco57I CTGAAG 4 cut(s) 360, 642, 732, 818
Eco88I CYCGRG 1 cut(s) 917
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 59
EcoRII CCWGG 1 cut(s) 229
EcoRV GATATC 2 cut(s) 796, 950
EcoT14I CCWWGG 3 cut(s) 455, 807, 1109
ErhI CCWWGG 3 cut(s) 455, 807, 1109
FaeI CATG 2 cut(s) 539, 1113
FalI AAGNNNNNCTT 6 cut(s) 308, 340, 992, 1024, 1052, 1084
FaqI GGGAC 2 cut(s) 160, 546
FatI CATG 2 cut(s) 535, 1109
FbaI TGATCA 2 cut(s) 3, 1018
Fnu4HI GCNGC 7 cut(s) 117, 150, 501, 765, 894, 897, 908
FokI GGATG 2 cut(s) 1127, 1157
Fsp4HI GCNGC 7 cut(s) 117, 150, 501, 765, 894, 897, 908
FspAI RTGCGCAY 1 cut(s) 168
FspBI CTAG 1 cut(s) 689
FspI TGCGCA 1 cut(s) 168
GlaI GCGC 2 cut(s) 69, 168
GluI GCNGC 7 cut(s) 117, 150, 501, 765, 894, 897, 908
GsaI CCCAGC 1 cut(s) 1050
GsuI CTGGAG 2 cut(s) 225, 363
HaeIII GGCC 2 cut(s) 140, 732
HhaI GCGC 2 cut(s) 70, 169
Hin1II CATG 2 cut(s) 539, 1113
Hin6I GCGC 2 cut(s) 68, 167
HinP1I GCGC 2 cut(s) 68, 167
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HinfI GANTC 9 cut(s) 17, 35, 97, 204, 588, 668, 803, 871, 922
HphI GGTGA 1 cut(s) 535
Hpy166II GTNNAC 2 cut(s) 80, 541
Hpy188I TCNGA 3 cut(s) 96, 1023, 1077
Hpy188III TCNNGA 7 cut(s) 32, 185, 208, 680, 710, 865, 919
Hpy8I GTNNAC 2 cut(s) 80, 541
HpyAV CCTTC 7 cut(s) 124, 310, 490, 508, 809, 842, 1018
HpyCH4III ACNGT 4 cut(s) 124, 145, 470, 545
HpyCH4V TGCA 6 cut(s) 281, 290, 452, 551, 704, 854
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 158, 287, 653, 962
HpyF3I CTNAG 4 cut(s) 183, 486, 720, 1182
Hsp92II CATG 2 cut(s) 539, 1113
HspAI GCGC 2 cut(s) 68, 167
Ksp22I TGATCA 2 cut(s) 3, 1018
Kzo9I GATC 6 cut(s) 3, 347, 427, 1018, 1023, 1131
LguI GCTCTTC 1 cut(s) 1094
LmnI GCTCC 2 cut(s) 257, 611
Lsp1109I GCAGC 6 cut(s) 136, 487, 751, 905, 908, 919
LweI GCATC 3 cut(s) 148, 154, 538
MaeI CTAG 1 cut(s) 689
MaeIII GTNAC 1 cut(s) 715
MalI GATC 6 cut(s) 5, 349, 429, 1020, 1025, 1133
MboI GATC 6 cut(s) 3, 347, 427, 1018, 1023, 1131
MboII GAAGA 6 cut(s) 505, 758, 880, 1111, 1151, 1187
MfeI CAATTG 1 cut(s) 663
MflI RGATCY 1 cut(s) 427
MhlI GDGCHC 1 cut(s) 468
MlsI TGGCCA 1 cut(s) 140
MluCI AATT 9 cut(s) 59, 284, 388, 397, 407, 476, 663, 1031, 1196
MluNI TGGCCA 1 cut(s) 140
MlyI GAGTC 3 cut(s) 29, 91, 880
Mox20I TGGCCA 1 cut(s) 140
MscI TGGCCA 1 cut(s) 140
MseI TTAA 5 cut(s) 303, 326, 417, 968, 999
MslI CAYNNNNRTG 2 cut(s) 457, 487
Msp20I TGGCCA 1 cut(s) 140
MspA1I CMGCKG 1 cut(s) 899
MspCI CTTAAG 2 cut(s) 325, 998
MspR9I CCNGG 1 cut(s) 231
MunI CAATTG 1 cut(s) 663
MvaI CCWGG 1 cut(s) 231
MvnI CGCG 1 cut(s) 927
MwoI GCNNNNNNNGC 5 cut(s) 146, 158, 287, 653, 962
NcoI CCATGG 1 cut(s) 1109
NdeII GATC 6 cut(s) 3, 347, 427, 1018, 1023, 1131
NlaIII CATG 2 cut(s) 539, 1113
NlaIV GGNNCC 2 cut(s) 483, 1084
NmuCI GTSAC 1 cut(s) 715
NsbI TGCGCA 1 cut(s) 168
NspI RCATGY 1 cut(s) 539
OliI CACNNNNGTG 2 cut(s) 457, 487
PaeR7I CTCGAG 1 cut(s) 917
PciI ACATGT 1 cut(s) 535
PciSI GCTCTTC 1 cut(s) 1094
PcsI WCGNNNNNNNCGW 1 cut(s) 924
PfeI GAWTC 6 cut(s) 17, 204, 588, 668, 803, 922
PflFI GACNNNGTC 1 cut(s) 84
PflMI CCANNNNNTGG 1 cut(s) 1115
PkrI GCNGC 7 cut(s) 118, 151, 502, 766, 895, 898, 909
PleI GAGTC 3 cut(s) 29, 91, 879
PpsI GAGTC 3 cut(s) 29, 91, 879
PscI ACATGT 1 cut(s) 535
Psp6I CCWGG 1 cut(s) 229
PspFI CCCAGC 1 cut(s) 1046
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 2 cut(s) 483, 1084
PspPI GGNCC 2 cut(s) 319, 731
PstNI CAGNNNCTG 1 cut(s) 899
PsuI RGATCY 1 cut(s) 427
PsyI GACNNNGTC 1 cut(s) 84
PvuII CAGCTG 1 cut(s) 899
RsaI GTAC 2 cut(s) 228, 633
RsaNI GTAC 2 cut(s) 227, 632
RseI CAYNNNNRTG 2 cut(s) 457, 487
SapI GCTCTTC 1 cut(s) 1094
SaqAI TTAA 5 cut(s) 303, 326, 417, 968, 999
SatI GCNGC 7 cut(s) 117, 150, 501, 765, 894, 897, 908
Sau3AI GATC 6 cut(s) 3, 347, 427, 1018, 1023, 1131
Sau96I GGNCC 2 cut(s) 319, 731
SchI GAGTC 3 cut(s) 29, 91, 880
ScrFI CCNGG 1 cut(s) 231
SduI GDGCHC 1 cut(s) 468
SfaNI GCATC 3 cut(s) 148, 154, 538
Sfr274I CTCGAG 1 cut(s) 917
SinI GGWCC 1 cut(s) 319
SlaI CTCGAG 1 cut(s) 917
SmiMI CAYNNNNRTG 2 cut(s) 457, 487
SmlI CTYRAG 3 cut(s) 325, 917, 998
SmoI CTYRAG 3 cut(s) 325, 917, 998
Sse9I AATT 9 cut(s) 59, 284, 388, 397, 407, 476, 663, 1031, 1196
SsiI CCGC 2 cut(s) 116, 322
SspI AATATT 1 cut(s) 600
SspMI CTAG 1 cut(s) 689
StyD4I CCNGG 1 cut(s) 229
StyI CCWWGG 3 cut(s) 455, 807, 1109
TaaI ACNGT 4 cut(s) 124, 145, 470, 545
TaqI TCGA 5 cut(s) 38, 681, 918, 1011, 1029
TasI AATT 9 cut(s) 59, 284, 388, 397, 407, 476, 663, 1031, 1196
TauI GCSGC 1 cut(s) 119
TfiI GAWTC 6 cut(s) 17, 204, 588, 668, 803, 922
Tru1I TTAA 5 cut(s) 303, 326, 417, 968, 999
Tru9I TTAA 5 cut(s) 303, 326, 417, 968, 999
TscAI CASTG 1 cut(s) 473
TseFI GTSAC 1 cut(s) 715
TseI GCWGC 6 cut(s) 149, 500, 764, 893, 896, 907
Tsp45I GTSAC 1 cut(s) 715
TspDTI ATGAA 2 cut(s) 39, 420
TspGWI ACGGA 1 cut(s) 1140
TspRI CASTG 1 cut(s) 473
Tth111I GACNNNGTC 1 cut(s) 84
Van91I CCANNNNNTGG 1 cut(s) 1115
Vha464I CTTAAG 2 cut(s) 325, 998
VpaK11BI GGWCC 1 cut(s) 319
XagI CCTNNNNNAGG 1 cut(s) 182
XapI RAATTY 5 cut(s) 59, 284, 388, 397, 1031
XceI RCATGY 1 cut(s) 539
XcmI CCANNNNNNNNNTGG 1 cut(s) 775
XhoI CTCGAG 1 cut(s) 917
XspI CTAG 1 cut(s) 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.