Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0014581

Physical Location & Seq

Genomic Coordinates Forward (+)

8924307 .. 8927432

Gene Structure 3126 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1116100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1308 bp

ATGAGAGAGAACCAATTTCGAGCAGTCGCAATCATCTTCTTCCTGTTTTTCTTCGTCTTCAATGTCTGTGATGCACTGTACGGACCTTCAACGCCAGTTCTTCAGCTAACTCCTTCCAACTTCAAGTCCAAGGTTCTGGACTCGAACAGAGTTGTTCTGGTTGAATTCTTTGCACCGTGGTGCGGGCATTGTCAGGCTCTAACACCGATATGGGAGAAGGCGGCTACTGTCTTAAAGGGTGTGGCTACCGTGGCAGCTCTTGATGCTGATGCGCACAAGTCGCTCGCTCAGGAATACGGGATCAGAGGATTTCCAACAATAAAGGTGTTTGTACCTGGAAAGCCTCCAGTTGACTATCAGGGAGCAAGGGATGTGAAACCCGTTGCAGAATTTGCACTCCAACAGATAAAGGCACTGTTGAAAGACCGTTTAAGTGGAAAGACAACAGGAGGATCAAGTGAAAAATCTGAACCTAGTGCTTCAGTAGAATTAAACTCCCAGAATTTTAATGAACTGGTGCTTAAAAGTAAGGAACTCTGGATTGTCGAGTTCTTTGCACCTTGGTGCGGGCACTGCAAAAAATTGGCACCTGAGTGGAAGAAGGCGGCTAAAAACTTACAAGGGAAGGTGAAGTTGGGACACGTTGATTGTGATGCAGAGAAGTCTCTGATGAGCAAGTTCAACGTTCAAGGATTTCCAACCATATTGGTATTTGGAGCTGACAAAGAGAGCCCACTCCCCTATGAGGGTGCGAGGAGTGCTTCAGCCATTGAATCATTTGCTCTTGAACAATTGGAAACAAACGTTGCACCTCCTGAAGTGACCGAGCTAACTGGCCCTGATGCAATGGAAGAGAAATGTGGTTCAGCTGCCATCTGTTTTGTGGCCTTCCTACCTGATATCTTGGACTCCAAGGCAGAAGGGAGGAACAAGTACATTCAGCAGTTACTATCCGTTGCAGAGAAGTTTAAGAGAAGTCCCTACTGCTATGTCTGGGCAGCAGCTGGTAAGCAACTAGACCTTGAGAACCGAGTAGGTGTCGGAGGTTATGGATATCCAGCATTGGTGGCCTTAAATGCAAAAAAGGGAGCTTATGCACCACTTAAGAGTGCATTCGAGGTTGATCAGATTACAGAATTTGTGAGGGAAGCCGGGCGTGGAGGAAAGGGAAACTTGCCTCTGGAGGGCACCCCGAGCATTGTGAAGATCGAACCCTGGGATGGTAAAGACGGACAGATTCTTGAAGAGGACGAGTTCTCCCTTGAAGAACTGATGGGAGAATACACTACGAACAAGGACGAGTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003756 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005788 GO:0005794 GO:0005886 GO:0006457 GO:0006950 GO:0008150 GO:0009505 GO:0009987 GO:0012505 GO:0016020 GO:0016853 GO:0016860 GO:0016864 GO:0030312 GO:0031974 GO:0033554 GO:0034976 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051716 GO:0070013 GO:0071944 GO:0140096

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Thioredoxin

Protein Analysis

436

Amino Acids

47.71

Weight (kDa)

5.47

Isoelectric Point (pI)

31.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thioredoxin	PF00085	33 - 130	4.8e-29	Thioredoxin
Thioredoxin	PF00085	162 - 261	1.7e-30	Thioredoxin
Thioredoxin_2	PF13098	177 - 257	3.5e-07	Thioredoxin-like domain
Thioredoxin_6	PF13848	195 - 382	8.3e-10	Thioredoxin-like domain
Thioredox_PDIA6_C	PF24541	268 - 382	1.3e-08	PDIA6-like, C-terminal thioredoxin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014581)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04980 AT2G32920
fragaria_vesca	FvH4_6g41940
malus_domestica	MD09G1116100.v1.1 MD17G1107800.v1.1
prunus_persica	Prupe.3G208600_v2.0.a1
pyrus_communis	pycom09g04050
rosa_chinensis	RchiOBHm_Chr2g0157821
rosa_laevigata	RLG00000021023
rosa_multiflora	Rmu_sc0002441.1_g000014
rosa_roxburghii	Rroxscaffold_2G00091920
rosa_rugosa	Rorug02G0464000
rosa_samantha	Rh2AG529300 Rh2BG543400 Rh2CG513600 Rh2DG552200
rosa_wichuraiana	Rw2G043760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	273
AccB1I	GGYRCC	2 cut(s)	586, 1187
AciI	CCGC	4 cut(s)	183, 221, 567, 605
AclI	AACGTT	2 cut(s)	684, 804
AclWI	GGATC	2 cut(s)	308, 460
AcsI	RAATTY	4 cut(s)	164, 389, 502, 1136
AcuI	CTGAAG	4 cut(s)	86, 465, 747, 837
AfaI	GTAC	3 cut(s)	80, 333, 935
AfiI	CCNNNNNNNGG	6 cut(s)	182, 566, 745, 746, 1184, 1220
AflII	CTTAAG	1 cut(s)	1103
AflIII	ACRYGT	1 cut(s)	640
AjnI	CCWGG	2 cut(s)	334, 1214
AjuI	GAANNNNNNNTTGG	2 cut(s)	617, 649
AleI	CACNNNNGTG	3 cut(s)	178, 562, 592
AluBI	AGCT	7 cut(s)	106, 257, 719, 829, 869, 1004, 1091
AluI	AGCT	7 cut(s)	106, 257, 719, 829, 869, 1004, 1091
Alw26I	GTCTC	1 cut(s)	669
AlwI	GGATC	2 cut(s)	308, 460
AlwNI	CAGNNNCTG	1 cut(s)	1004
Ama87I	CYCGRG	1 cut(s)	1192
AoxI	GGCC	3 cut(s)	835, 885, 1068
ApeKI	GCWGC	4 cut(s)	254, 869, 998, 1001
ApoI	RAATTY	4 cut(s)	164, 389, 502, 1136
AspLEI	GCGC	1 cut(s)	274
AspS9I	GGNCC	2 cut(s)	83, 836
AsuC2I	CCSGG	1 cut(s)	1153
AsuHPI	GGTGA	1 cut(s)	640
AvaI	CYCGRG	1 cut(s)	1192
AvaII	GGWCC	1 cut(s)	83
BaeGI	GKGCMC	2 cut(s)	573, 1190
BanI	GGYRCC	2 cut(s)	586, 1187
BanII	GRGCYC	1 cut(s)	734
BbsI	GAAGAC	1 cut(s)	49
BbvI	GCAGC	4 cut(s)	266, 856, 1010, 1013
BccI	CCATC	3 cut(s)	881, 1214, 1267
BcgI	CGANNNNNNTGC	2 cut(s)	536, 570
BciT130I	CCWGG	2 cut(s)	336, 1216
BclI	TGATCA	1 cut(s)	1123
BcnI	CCSGG	1 cut(s)	1153
BcoDI	GTCTC	1 cut(s)	669
BfaI	CTAG	2 cut(s)	474, 1016
BfrI	CTTAAG	1 cut(s)	1103
BisI	GCNGC	6 cut(s)	222, 255, 606, 870, 999, 1002
BlsI	GCNGC	6 cut(s)	223, 256, 607, 871, 1000, 1003
Bme1390I	CCNGG	3 cut(s)	336, 1153, 1216
Bme18I	GGWCC	1 cut(s)	83
BmeT110I	CYCGRG	1 cut(s)	1192
BmgT120I	GGNCC	2 cut(s)	83, 836
BmiI	GGNNCC	2 cut(s)	588, 1189
BmrFI	CCNGG	3 cut(s)	336, 1153, 1216
BmsI	GCATC	5 cut(s)	61, 253, 259, 643, 832
BpiI	GAAGAC	1 cut(s)	49
BpmI	CTGGAG	2 cut(s)	330, 1202
Bpu10I	CCTNAGC	1 cut(s)	288
BpuEI	CTTGAG	1 cut(s)	1043
BpuMI	CCSGG	1 cut(s)	1153
BsaJI	CCNNGG	7 cut(s)	129, 176, 249, 560, 912, 1214, 1215
BsaXI	ACNNNNNCTCC	2 cut(s)	1241, 1271
Bsc4I	CCNNNNNNNGG	6 cut(s)	182, 566, 745, 746, 1184, 1220
Bse1I	ACTGG	4 cut(s)	95, 347, 519, 838
Bse3DI	GCAATG	1 cut(s)	852
BseBI	CCWGG	2 cut(s)	336, 1216
BseDI	CCNNGG	7 cut(s)	129, 176, 249, 560, 912, 1214, 1215
BseGI	GGATG	2 cut(s)	376, 1225
BseLI	CCNNNNNNNGG	6 cut(s)	182, 566, 745, 746, 1184, 1220
BseMI	GCAATG	1 cut(s)	852
BseMII	CTCAG	2 cut(s)	302, 582
BseNI	ACTGG	4 cut(s)	95, 347, 519, 838
BseRI	GAGGAG	1 cut(s)	769
BseSI	GKGCMC	2 cut(s)	573, 1190
BseXI	GCAGC	4 cut(s)	266, 856, 1010, 1013
BshFI	GGCC	3 cut(s)	837, 887, 1070
BshNI	GGYRCC	2 cut(s)	586, 1187
BsiHKCI	CYCGRG	1 cut(s)	1192
BsiSI	CCGG	1 cut(s)	1152
BslFI	GGGAC	2 cut(s)	651, 963
BslI	CCNNNNNNNGG	6 cut(s)	182, 566, 745, 746, 1184, 1220
BsmAI	GTCTC	1 cut(s)	669
BsmFI	GGGAC	2 cut(s)	651, 963
BsmI	GAATGC	1 cut(s)	1112
BsnI	GGCC	3 cut(s)	837, 887, 1070
BsoBI	CYCGRG	1 cut(s)	1192
Bsp1286I	GDGCHC	3 cut(s)	573, 734, 1190
Bsp143I	GATC	4 cut(s)	300, 452, 1123, 1206
BspACI	CCGC	4 cut(s)	183, 221, 567, 605
BspANI	GGCC	3 cut(s)	837, 887, 1070
BspCNI	CTCAG	2 cut(s)	301, 583
BspLI	GGNNCC	2 cut(s)	588, 1189
BspPI	GGATC	2 cut(s)	308, 460
BspT107I	GGYRCC	2 cut(s)	586, 1187
BspTI	CTTAAG	1 cut(s)	1103
BsrDI	GCAATG	1 cut(s)	852
BsrI	ACTGG	4 cut(s)	95, 347, 519, 838
BssECI	CCNNGG	7 cut(s)	129, 176, 249, 560, 912, 1214, 1215
BssMI	GATC	4 cut(s)	300, 452, 1123, 1206
BssT1I	CCWWGG	3 cut(s)	129, 560, 912
Bst2UI	CCWGG	2 cut(s)	336, 1216
Bst4CI	ACNGT	6 cut(s)	78, 177, 229, 250, 417, 428
Bst6I	CTCTTC	2 cut(s)	846, 1239
BstAFI	CTTAAG	1 cut(s)	1103
BstAPI	GCANNNNNTGC	1 cut(s)	392
BstC8I	GCNNGC	3 cut(s)	185, 285, 569
BstDEI	CTNAG	2 cut(s)	288, 591
BstDSI	CCRYGG	2 cut(s)	176, 249
BstF5I	GGATG	2 cut(s)	376, 1225
BstHHI	GCGC	1 cut(s)	274
BstKTI	GATC	4 cut(s)	303, 455, 1126, 1209
BstMAI	GTCTC	1 cut(s)	669
BstMBI	GATC	4 cut(s)	300, 452, 1123, 1206
BstMWI	GCNNNNNNNGC	9 cut(s)	251, 263, 280, 392, 573, 758, 1067, 1076, 1194
BstNI	CCWGG	2 cut(s)	336, 1216
BstSCI	CCNGG	3 cut(s)	334, 1151, 1214
BstSLI	GKGCMC	2 cut(s)	573, 1190
BstV1I	GCAGC	4 cut(s)	266, 856, 1010, 1013
BstV2I	GAAGAC	1 cut(s)	49
BstXI	CCANNNNNNTGG	1 cut(s)	136
BsuRI	GGCC	3 cut(s)	837, 887, 1070
BtgI	CCRYGG	2 cut(s)	176, 249
BtsCI	GGATG	2 cut(s)	376, 1225
BtsI	GCAGTG	1 cut(s)	571
BtsIMutI	CAGTG	3 cut(s)	74, 413, 571
Cac8I	GCNNGC	3 cut(s)	185, 285, 569
CaiI	CAGNNNCTG	1 cut(s)	1004
CfoI	GCGC	1 cut(s)	274
Cfr13I	GGNCC	2 cut(s)	83, 836
Csp6I	GTAC	3 cut(s)	79, 332, 934
CviQI	GTAC	3 cut(s)	79, 332, 934
DdeI	CTNAG	2 cut(s)	288, 591
DpnI	GATC	4 cut(s)	302, 454, 1125, 1208
DpnII	GATC	4 cut(s)	300, 452, 1123, 1206
Eam1104I	CTCTTC	2 cut(s)	846, 1239
EarI	CTCTTC	2 cut(s)	846, 1239
Eco130I	CCWWGG	3 cut(s)	129, 560, 912
Eco24I	GRGCYC	1 cut(s)	734
Eco32I	GATATC	2 cut(s)	901, 1055
Eco47I	GGWCC	1 cut(s)	83
Eco57I	CTGAAG	4 cut(s)	86, 465, 747, 837
Eco88I	CYCGRG	1 cut(s)	1192
EcoRI	GAATTC	1 cut(s)	164
EcoRII	CCWGG	2 cut(s)	334, 1214
EcoRV	GATATC	2 cut(s)	901, 1055
EcoT14I	CCWWGG	3 cut(s)	129, 560, 912
EcoT38I	GRGCYC	1 cut(s)	734
ErhI	CCWWGG	3 cut(s)	129, 560, 912
FaiI	YATR	6 cut(s)	211, 704, 744, 990, 1050, 1095
FalI	AAGNNNNNCTT	2 cut(s)	1157, 1189
FaqI	GGGAC	2 cut(s)	651, 963
FauI	CCCGC	2 cut(s)	176, 560
FbaI	TGATCA	1 cut(s)	1123
Fnu4HI	GCNGC	6 cut(s)	222, 255, 606, 870, 999, 1002
FokI	GGATG	2 cut(s)	383, 1232
FriOI	GRGCYC	1 cut(s)	734
Fsp4HI	GCNGC	6 cut(s)	222, 255, 606, 870, 999, 1002
FspAI	RTGCGCAY	1 cut(s)	273
FspBI	CTAG	2 cut(s)	474, 1016
FspI	TGCGCA	1 cut(s)	273
GlaI	GCGC	1 cut(s)	273
GluI	GCNGC	6 cut(s)	222, 255, 606, 870, 999, 1002
GsuI	CTGGAG	2 cut(s)	330, 1202
HaeIII	GGCC	3 cut(s)	837, 887, 1070
HapII	CCGG	1 cut(s)	1152
HhaI	GCGC	1 cut(s)	274
Hin6I	GCGC	1 cut(s)	272
HinP1I	GCGC	1 cut(s)	272
HincII	GTYRAC	1 cut(s)	352
HindII	GTYRAC	1 cut(s)	352
HinfI	GANTC	4 cut(s)	140, 773, 908, 1237
HpaII	CCGG	1 cut(s)	1152
HphI	GGTGA	1 cut(s)	640
Hpy166II	GTNNAC	1 cut(s)	352
Hpy188I	TCNGA	5 cut(s)	305, 469, 669, 1043, 1128
Hpy188III	TCNNGA	8 cut(s)	137, 260, 290, 538, 785, 815, 1181, 1241
Hpy8I	GTNNAC	1 cut(s)	352
HpyAV	CCTTC	7 cut(s)	96, 123, 211, 595, 619, 898, 914
HpyCH4III	ACNGT	6 cut(s)	78, 177, 229, 250, 417, 428
HpyCH4IV	ACGT	3 cut(s)	642, 684, 804
HpyF10VI	GCNNNNNNNGC	9 cut(s)	251, 263, 280, 392, 573, 758, 1067, 1076, 1194
HpyF3I	CTNAG	2 cut(s)	288, 591
HpySE526I	ACGT	3 cut(s)	642, 684, 804
HspAI	GCGC	1 cut(s)	272
Ksp22I	TGATCA	1 cut(s)	1123
Kzo9I	GATC	4 cut(s)	300, 452, 1123, 1206
LmnI	GCTCC	3 cut(s)	362, 716, 1088
Lsp1109I	GCAGC	4 cut(s)	266, 856, 1010, 1013
LweI	GCATC	5 cut(s)	61, 253, 259, 643, 832
MaeI	CTAG	2 cut(s)	474, 1016
MaeII	ACGT	3 cut(s)	642, 684, 804
MaeIII	GTNAC	2 cut(s)	820, 945
MalI	GATC	4 cut(s)	302, 454, 1125, 1208
MboI	GATC	4 cut(s)	300, 452, 1123, 1206
MfeI	CAATTG	1 cut(s)	791
MhlI	GDGCHC	3 cut(s)	573, 734, 1190
MluCI	AATT	8 cut(s)	14, 164, 389, 488, 502, 581, 791, 1136
MlyI	GAGTC	2 cut(s)	134, 902
MmeI	TCCRAC	5 cut(s)	141, 338, 424, 722, 1021
MseI	TTAA	8 cut(s)	233, 431, 491, 507, 522, 969, 1073, 1104
MslI	CAYNNNNRTG	4 cut(s)	178, 208, 562, 592
MspA1I	CMGCKG	2 cut(s)	869, 1004
MspCI	CTTAAG	1 cut(s)	1103
MspI	CCGG	1 cut(s)	1152
MspR9I	CCNGG	3 cut(s)	336, 1153, 1216
MunI	CAATTG	1 cut(s)	791
Mva1269I	GAATGC	1 cut(s)	1112
MvaI	CCWGG	2 cut(s)	336, 1216
MwoI	GCNNNNNNNGC	9 cut(s)	251, 263, 280, 392, 573, 758, 1067, 1076, 1194
NciI	CCSGG	1 cut(s)	1153
NdeII	GATC	4 cut(s)	300, 452, 1123, 1206
NlaIV	GGNNCC	2 cut(s)	588, 1189
NmuCI	GTSAC	1 cut(s)	820
NsbI	TGCGCA	1 cut(s)	273
OliI	CACNNNNGTG	3 cut(s)	178, 562, 592
PasI	CCCWGGG	1 cut(s)	1215
PctI	GAATGC	1 cut(s)	1112
PfeI	GAWTC	2 cut(s)	773, 1237
PkrI	GCNGC	6 cut(s)	223, 256, 607, 871, 1000, 1003
PleI	GAGTC	2 cut(s)	134, 902
PpsI	GAGTC	2 cut(s)	134, 902
Psp1406I	AACGTT	2 cut(s)	684, 804
Psp6I	CCWGG	2 cut(s)	334, 1214
PspGI	CCWGG	2 cut(s)	334, 1214
PspN4I	GGNNCC	2 cut(s)	588, 1189
PspPI	GGNCC	2 cut(s)	83, 836
PstNI	CAGNNNCTG	1 cut(s)	1004
PvuII	CAGCTG	2 cut(s)	869, 1004
RsaI	GTAC	3 cut(s)	80, 333, 935
RsaNI	GTAC	3 cut(s)	79, 332, 934
RseI	CAYNNNNRTG	4 cut(s)	178, 208, 562, 592
SaqAI	TTAA	8 cut(s)	233, 431, 491, 507, 522, 969, 1073, 1104
SatI	GCNGC	6 cut(s)	222, 255, 606, 870, 999, 1002
Sau3AI	GATC	4 cut(s)	300, 452, 1123, 1206
Sau96I	GGNCC	2 cut(s)	83, 836
SchI	GAGTC	2 cut(s)	134, 902
ScrFI	CCNGG	3 cut(s)	336, 1153, 1216
SduI	GDGCHC	3 cut(s)	573, 734, 1190
SfaNI	GCATC	5 cut(s)	61, 253, 259, 643, 832
SinI	GGWCC	1 cut(s)	83
SmiMI	CAYNNNNRTG	4 cut(s)	178, 208, 562, 592
SmlI	CTYRAG	2 cut(s)	1022, 1103
SmoI	CTYRAG	2 cut(s)	1022, 1103
Sse9I	AATT	8 cut(s)	14, 164, 389, 488, 502, 581, 791, 1136
SsiI	CCGC	4 cut(s)	183, 221, 567, 605
SspMI	CTAG	2 cut(s)	474, 1016
StyD4I	CCNGG	3 cut(s)	334, 1151, 1214
StyI	CCWWGG	3 cut(s)	129, 560, 912
TaaI	ACNGT	6 cut(s)	78, 177, 229, 250, 417, 428
TaiI	ACGT	3 cut(s)	645, 687, 807
TaqI	TCGA	5 cut(s)	19, 143, 546, 1116, 1209
TaqII	GACCGA	1 cut(s)	839
TasI	AATT	8 cut(s)	14, 164, 389, 488, 502, 581, 791, 1136
TatI	WGTACW	1 cut(s)	933
TauI	GCSGC	2 cut(s)	224, 608
TfiI	GAWTC	2 cut(s)	773, 1237
Tru1I	TTAA	8 cut(s)	233, 431, 491, 507, 522, 969, 1073, 1104
Tru9I	TTAA	8 cut(s)	233, 431, 491, 507, 522, 969, 1073, 1104
TscAI	CASTG	3 cut(s)	81, 420, 578
TseFI	GTSAC	1 cut(s)	820
TseI	GCWGC	4 cut(s)	254, 869, 998, 1001
Tsp45I	GTSAC	1 cut(s)	820
TspDTI	ATGAA	1 cut(s)	525
TspGWI	ACGGA	3 cut(s)	96, 943, 1245
TspRI	CASTG	3 cut(s)	81, 420, 578
Vha464I	CTTAAG	1 cut(s)	1103
VpaK11BI	GGWCC	1 cut(s)	83
XapI	RAATTY	4 cut(s)	164, 389, 502, 1136
XcmI	CCANNNNNNNNNTGG	1 cut(s)	880
XspI	CTAG	2 cut(s)	474, 1016

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.