MD17G1107800.v1.1

Belongs to the protein disulfide isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014581
Physical Location & Seq
Forward (+)
9268152 .. 9271369
3218 bp
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UTR
Exon/CDS
Intron
MD17G1107800.v1.1.491

Sequence Viewer

Length: 1305 bp
ATGAGAGAGAATCAATTTCGAGCAGTCCCAATCATCTTCTTGTTTTTCTTCGTCTTCAGTGTCTGTGATGCACTATACGGACCATCATCGCCAGTTCTTCAGCTAACTCCTTCCAACTTCAAGTCCAAGGTTCTGGACTCAAACAGAGTTGTTCTGGTTGAATTCTTTGCACCGTGGTGCGGACATTGTCAGGCTCTTACACCGATATGGGAGAAAGCGGCTACTGTCTTAAAGGGTGTGGTTGCCGTGGCAGCTCTTGATGCTGATGCACACAAGTCCCTCGCTCAGGAATATGGGATTAGAGGATTTCCAACAATAAAGGTGTTTGTACCCGGAAAGCCTCCAGTTGACTATCAGGGAGCAAGGGATGTGAAACCCATTGCAGAATTTGCACTCCAACAGATAAAGGCGCTGTTGAAAGACCGCTTAAGTGGAAAAACAACAGGAGGATCAAGCGAAAAATCTGAACCTAGTGCTTCGGTAGAATTAAATTCCCAGAATTTTAATGAACTGGTGCTTAAAAGTAAAGATCTCTGGATTGTGGAGTTCTTTGCACCTTGGTGTGGGCACTGCAAAAAATTGGCACCTGAGTGGAAGAAGGCCGCTAAAAACTTGCAGGGGAAGGTGAAGTTGGGACACGTTGACTGTGATGCAGAGAAGTCTCTGATGAGCAAGTTCAACGTTCAAGGATTTCCGACCATATTGGTATTTGGAGCTGACAAAGACAGCCCACTCCCCTATGAGGGTGCAAGGAGTGCTTCAGCCATTGAATCATTTGCTCTCGAACAATTGGAAACAAACGTTGCACCTCCTGAAGTGACCGAGCTAACTGGCCCAGATGTCATGGAAGAGAAATGTGGTTCAGCTGCCATCTGTTTTGTGGCTTTCCTACCTGATATTTTGGATTCCAAGGCAGAAGGGAGGAACAAGTACATTCAGCAGTTATTATCCGTTGCGGAGAAGTTTAAAAGAAGTCCATACAGCTTTGTGTGGGCAGCAGCTGGTAAGCAGCCAGACCTCGAGAACCGAGTGGGTGTCGGTGGTTATGGATATCCAGCATTGGTGGCCTTAAACGCAAAAAAGGGAGCTTACGCACCACTTAAGAGTGCATTCGAGGTTGATCAGATCACAGAATTTGTGAAGGAAGCCGGGCGCGGAGGAAAGGGAAACTTGCCCCTGGAGGGCACCCCGAACATTGTGAAGATCGAACCCTGGGATGGTAAAGACGGAGAGATAATTGAAGAGGACGAGTTCTCCCTTGACGAACTAATGGGAGAAGATACCACTAACAAGGAAGAGTTGTGA

Protein Analysis

435

Amino Acids

47.46

Weight (kDa)

5.29

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 32 - 130 9.5e-30 Thioredoxin
OST3_OST6 PF04756 156 - 249 6.1e-06 OST3 / OST6 family, transporter family
Thioredoxin PF00085 161 - 260 2.1e-30 Thioredoxin
Thioredoxin_2 PF13098 177 - 255 8e-07 Thioredoxin-like domain
Thioredoxin_6 PF13848 194 - 382 2.8e-12 Thioredoxin-like domain
Thioredox_PDIA6_C PF24541 267 - 382 1.6e-09 PDIA6-like, C-terminal thioredoxin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014581)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04980 AT2G32920
fragaria_vesca FvH4_6g41940
malus_domestica MD09G1116100.v1.1 MD17G1107800.v1.1
prunus_persica Prupe.3G208600_v2.0.a1
pyrus_communis pycom09g04050
rosa_chinensis RchiOBHm_Chr2g0157821
rosa_laevigata RLG00000021023
rosa_multiflora Rmu_sc0002441.1_g000014
rosa_roxburghii Rroxscaffold_2G00091920
rosa_rugosa Rorug02G0464000
rosa_samantha Rh2AG529300 Rh2BG543400 Rh2CG513600 Rh2DG552200
rosa_wichuraiana Rw2G043760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 583, 1184
AccII CGCG 1 cut(s) 1155
AciI CCGC 6 cut(s) 180, 218, 424, 603, 956, 1155
AclI AACGTT 2 cut(s) 681, 801
AclWI GGATC 1 cut(s) 457
AcsI RAATTY 5 cut(s) 161, 386, 490, 499, 1133
AcuI CTGAAG 4 cut(s) 40, 83, 744, 834
AfaI GTAC 2 cut(s) 330, 932
AfiI CCNNNNNNNGG 7 cut(s) 179, 286, 563, 742, 743, 1181, 1217
AflII CTTAAG 2 cut(s) 427, 1100
AflIII ACRYGT 1 cut(s) 637
AgsI TTSAA 7 cut(s) 121, 161, 418, 679, 686, 770, 1241
AjnI CCWGG 2 cut(s) 1176, 1211
AjuI GAANNNNNNNTTGG 2 cut(s) 614, 646
AleI CACNNNNGTG 3 cut(s) 175, 559, 589
AluBI AGCT 8 cut(s) 103, 254, 716, 826, 866, 984, 1001, 1088
AluI AGCT 8 cut(s) 103, 254, 716, 826, 866, 984, 1001, 1088
Alw26I GTCTC 1 cut(s) 666
AlwI GGATC 1 cut(s) 457
AlwNI CAGNNNCTG 2 cut(s) 63, 1001
Ama87I CYCGRG 1 cut(s) 1019
AoxI GGCC 3 cut(s) 600, 832, 1065
ApeKI GCWGC 5 cut(s) 251, 866, 995, 998, 1009
ApoI RAATTY 5 cut(s) 161, 386, 490, 499, 1133
AspLEI GCGC 2 cut(s) 412, 1155
AspS9I GGNCC 2 cut(s) 80, 833
AsuC2I CCSGG 2 cut(s) 333, 1150
AsuHPI GGTGA 1 cut(s) 637
AvaI CYCGRG 1 cut(s) 1019
AvaII GGWCC 1 cut(s) 80
BaeGI GKGCMC 2 cut(s) 570, 1187
BanI GGYRCC 2 cut(s) 583, 1184
BbsI GAAGAC 1 cut(s) 46
BbvI GCAGC 5 cut(s) 263, 853, 1007, 1010, 1021
BccI CCATC 3 cut(s) 91, 878, 1211
BceAI ACGGC 1 cut(s) 230
BciT130I CCWGG 2 cut(s) 1178, 1213
BclI TGATCA 1 cut(s) 1120
BcnI CCSGG 2 cut(s) 333, 1150
BcoDI GTCTC 1 cut(s) 666
BfaI CTAG 1 cut(s) 471
BfoI RGCGCY 1 cut(s) 413
BfrI CTTAAG 2 cut(s) 427, 1100
BglII AGATCT 1 cut(s) 529
BisI GCNGC 7 cut(s) 219, 252, 603, 867, 996, 999, 1010
BlsI GCNGC 7 cut(s) 220, 253, 604, 868, 997, 1000, 1011
Bme1390I CCNGG 4 cut(s) 333, 1150, 1178, 1213
Bme18I GGWCC 1 cut(s) 80
BmeT110I CYCGRG 1 cut(s) 1019
BmgT120I GGNCC 2 cut(s) 80, 833
BmiI GGNNCC 2 cut(s) 585, 1186
BmrFI CCNGG 4 cut(s) 333, 1150, 1178, 1213
BmsI GCATC 4 cut(s) 58, 250, 256, 640
BpiI GAAGAC 1 cut(s) 46
BpmI CTGGAG 2 cut(s) 327, 1199
Bpu10I CCTNAGC 1 cut(s) 285
BpuMI CCSGG 2 cut(s) 333, 1150
BsaJI CCNNGG 8 cut(s) 126, 173, 246, 557, 909, 1176, 1211, 1212
BsaXI ACNNNNNCTCC 2 cut(s) 1238, 1268
Bsc4I CCNNNNNNNGG 7 cut(s) 179, 286, 563, 742, 743, 1181, 1217
Bse1I ACTGG 4 cut(s) 92, 344, 516, 835
Bse3DI GCAATG 1 cut(s) 378
BseBI CCWGG 2 cut(s) 1178, 1213
BseDI CCNNGG 8 cut(s) 126, 173, 246, 557, 909, 1176, 1211, 1212
BseGI GGATG 2 cut(s) 373, 1222
BseLI CCNNNNNNNGG 7 cut(s) 179, 286, 563, 742, 743, 1181, 1217
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 2 cut(s) 299, 579
BseNI ACTGG 4 cut(s) 92, 344, 516, 835
BseSI GKGCMC 2 cut(s) 570, 1187
BseXI GCAGC 5 cut(s) 263, 853, 1007, 1010, 1021
Bsh1236I CGCG 1 cut(s) 1155
BshFI GGCC 3 cut(s) 602, 834, 1067
BshNI GGYRCC 2 cut(s) 583, 1184
BsiHKCI CYCGRG 1 cut(s) 1019
BsiSI CCGG 2 cut(s) 333, 1149
BslFI GGGAC 3 cut(s) 11, 262, 648
BslI CCNNNNNNNGG 7 cut(s) 179, 286, 563, 742, 743, 1181, 1217
BsmAI GTCTC 1 cut(s) 666
BsmFI GGGAC 3 cut(s) 11, 262, 648
BsmI GAATGC 1 cut(s) 1109
BsnI GGCC 3 cut(s) 602, 834, 1067
BsoBI CYCGRG 1 cut(s) 1019
Bsp1286I GDGCHC 2 cut(s) 570, 1187
Bsp143I GATC 5 cut(s) 449, 529, 1120, 1125, 1203
BspACI CCGC 6 cut(s) 180, 218, 424, 603, 956, 1155
BspANI GGCC 3 cut(s) 602, 834, 1067
BspCNI CTCAG 2 cut(s) 298, 580
BspFNI CGCG 1 cut(s) 1155
BspLI GGNNCC 2 cut(s) 585, 1186
BspPI GGATC 1 cut(s) 457
BspT107I GGYRCC 2 cut(s) 583, 1184
BspTI CTTAAG 2 cut(s) 427, 1100
BsrDI GCAATG 1 cut(s) 378
BsrI ACTGG 4 cut(s) 92, 344, 516, 835
BssECI CCNNGG 8 cut(s) 126, 173, 246, 557, 909, 1176, 1211, 1212
BssMI GATC 5 cut(s) 449, 529, 1120, 1125, 1203
BssT1I CCWWGG 3 cut(s) 126, 557, 909
Bst2UI CCWGG 2 cut(s) 1178, 1213
Bst4CI ACNGT 3 cut(s) 174, 226, 647
Bst6I CTCTTC 3 cut(s) 843, 1236, 1290
BstAFI CTTAAG 2 cut(s) 427, 1100
BstAPI GCANNNNNTGC 2 cut(s) 389, 755
BstDEI CTNAG 2 cut(s) 285, 588
BstDSI CCRYGG 2 cut(s) 173, 246
BstENI CCTNNNNNAGG 1 cut(s) 284
BstF5I GGATG 2 cut(s) 373, 1222
BstFNI CGCG 1 cut(s) 1155
BstH2I RGCGCY 1 cut(s) 413
BstHHI GCGC 2 cut(s) 412, 1155
BstKTI GATC 5 cut(s) 452, 532, 1123, 1128, 1206
BstMAI GTCTC 1 cut(s) 666
BstMBI GATC 5 cut(s) 449, 529, 1120, 1125, 1203
BstMWI GCNNNNNNNGC 6 cut(s) 251, 260, 389, 755, 1064, 1073
BstNI CCWGG 2 cut(s) 1178, 1213
BstSCI CCNGG 4 cut(s) 331, 1148, 1176, 1211
BstSLI GKGCMC 2 cut(s) 570, 1187
BstUI CGCG 1 cut(s) 1155
BstV1I GCAGC 5 cut(s) 263, 853, 1007, 1010, 1021
BstV2I GAAGAC 1 cut(s) 46
BstX2I RGATCY 1 cut(s) 529
BstXI CCANNNNNNTGG 1 cut(s) 133
BstYI RGATCY 1 cut(s) 529
BsuRI GGCC 3 cut(s) 602, 834, 1067
BtgI CCRYGG 2 cut(s) 173, 246
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 2 cut(s) 373, 1222
BtsI GCAGTG 1 cut(s) 568
BtsIMutI CAGTG 2 cut(s) 64, 568
CaiI CAGNNNCTG 2 cut(s) 63, 1001
CfoI GCGC 2 cut(s) 412, 1155
Cfr13I GGNCC 2 cut(s) 80, 833
Csp6I GTAC 2 cut(s) 329, 931
CviAII CATG 1 cut(s) 844
CviQI GTAC 2 cut(s) 329, 931
DdeI CTNAG 2 cut(s) 285, 588
DpnI GATC 5 cut(s) 451, 531, 1122, 1127, 1205
DpnII GATC 5 cut(s) 449, 529, 1120, 1125, 1203
DraI TTTAAA 1 cut(s) 967
Eam1104I CTCTTC 3 cut(s) 843, 1236, 1290
EarI CTCTTC 3 cut(s) 843, 1236, 1290
Eco130I CCWWGG 3 cut(s) 126, 557, 909
Eco32I GATATC 1 cut(s) 1052
Eco47I GGWCC 1 cut(s) 80
Eco57I CTGAAG 4 cut(s) 40, 83, 744, 834
Eco88I CYCGRG 1 cut(s) 1019
EcoNI CCTNNNNNAGG 1 cut(s) 284
EcoRI GAATTC 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 1176, 1211
EcoRV GATATC 1 cut(s) 1052
EcoT14I CCWWGG 3 cut(s) 126, 557, 909
ErhI CCWWGG 3 cut(s) 126, 557, 909
FaeI CATG 1 cut(s) 847
FaiI YATR 8 cut(s) 76, 208, 294, 701, 741, 845, 979, 1047
FalI AAGNNNNNCTT 4 cut(s) 742, 774, 1154, 1186
FaqI GGGAC 3 cut(s) 11, 262, 648
FatI CATG 1 cut(s) 843
FbaI TGATCA 1 cut(s) 1120
Fnu4HI GCNGC 7 cut(s) 219, 252, 603, 867, 996, 999, 1010
FokI GGATG 2 cut(s) 380, 1229
Fsp4HI GCNGC 7 cut(s) 219, 252, 603, 867, 996, 999, 1010
FspBI CTAG 1 cut(s) 471
GlaI GCGC 2 cut(s) 411, 1154
GluI GCNGC 7 cut(s) 219, 252, 603, 867, 996, 999, 1010
GsuI CTGGAG 2 cut(s) 327, 1199
HaeII RGCGCY 1 cut(s) 413
HaeIII GGCC 3 cut(s) 602, 834, 1067
HapII CCGG 2 cut(s) 333, 1149
HhaI GCGC 2 cut(s) 412, 1155
Hin1II CATG 1 cut(s) 847
Hin6I GCGC 2 cut(s) 410, 1153
HinP1I GCGC 2 cut(s) 410, 1153
HincII GTYRAC 2 cut(s) 349, 643
HindII GTYRAC 2 cut(s) 349, 643
HinfI GANTC 4 cut(s) 10, 137, 770, 905
HpaII CCGG 2 cut(s) 333, 1149
HphI GGTGA 1 cut(s) 637
Hpy166II GTNNAC 2 cut(s) 349, 643
Hpy188I TCNGA 4 cut(s) 466, 666, 696, 1125
Hpy188III TCNNGA 7 cut(s) 134, 257, 287, 535, 782, 812, 1021
Hpy8I GTNNAC 2 cut(s) 349, 643
HpyAV CCTTC 5 cut(s) 120, 592, 616, 911, 1135
HpyCH4III ACNGT 3 cut(s) 174, 226, 647
HpyCH4IV ACGT 3 cut(s) 639, 681, 801
HpyF10VI GCNNNNNNNGC 6 cut(s) 251, 260, 389, 755, 1064, 1073
HpyF3I CTNAG 2 cut(s) 285, 588
HpySE526I ACGT 3 cut(s) 639, 681, 801
Hsp92II CATG 1 cut(s) 847
HspAI GCGC 2 cut(s) 410, 1153
Ksp22I TGATCA 1 cut(s) 1120
Kzo9I GATC 5 cut(s) 449, 529, 1120, 1125, 1203
LmnI GCTCC 3 cut(s) 359, 713, 1085
Lsp1109I GCAGC 5 cut(s) 263, 853, 1007, 1010, 1021
LweI GCATC 4 cut(s) 58, 250, 256, 640
MaeI CTAG 1 cut(s) 471
MaeII ACGT 3 cut(s) 639, 681, 801
MaeIII GTNAC 1 cut(s) 817
MalI GATC 5 cut(s) 451, 531, 1122, 1127, 1205
MboI GATC 5 cut(s) 449, 529, 1120, 1125, 1203
MboII GAAGA 9 cut(s) 28, 40, 46, 89, 607, 860, 1213, 1253, 1289
MfeI CAATTG 1 cut(s) 788
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 2 cut(s) 570, 1187
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 4 cut(s) 138, 335, 421, 719
MseI TTAA 8 cut(s) 230, 428, 488, 504, 519, 966, 1070, 1101
MslI CAYNNNNRTG 4 cut(s) 175, 205, 559, 589
MspA1I CMGCKG 2 cut(s) 866, 1001
MspCI CTTAAG 2 cut(s) 427, 1100
MspI CCGG 2 cut(s) 333, 1149
MspR9I CCNGG 4 cut(s) 333, 1150, 1178, 1213
MunI CAATTG 1 cut(s) 788
Mva1269I GAATGC 1 cut(s) 1109
MvaI CCWGG 2 cut(s) 1178, 1213
MvnI CGCG 1 cut(s) 1155
MwoI GCNNNNNNNGC 6 cut(s) 251, 260, 389, 755, 1064, 1073
NciI CCSGG 2 cut(s) 333, 1150
NdeII GATC 5 cut(s) 449, 529, 1120, 1125, 1203
NlaIII CATG 1 cut(s) 847
NlaIV GGNNCC 2 cut(s) 585, 1186
NmuCI GTSAC 1 cut(s) 817
OliI CACNNNNGTG 3 cut(s) 175, 559, 589
PaeR7I CTCGAG 1 cut(s) 1019
PasI CCCWGGG 1 cut(s) 1212
PctI GAATGC 1 cut(s) 1109
PfeI GAWTC 3 cut(s) 10, 770, 905
PflFI GACNNNGTC 1 cut(s) 186
PkrI GCNGC 7 cut(s) 220, 253, 604, 868, 997, 1000, 1011
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
Psp1406I AACGTT 2 cut(s) 681, 801
Psp6I CCWGG 2 cut(s) 1176, 1211
PspGI CCWGG 2 cut(s) 1176, 1211
PspN4I GGNNCC 2 cut(s) 585, 1186
PspPI GGNCC 2 cut(s) 80, 833
PstNI CAGNNNCTG 2 cut(s) 63, 1001
PsuI RGATCY 1 cut(s) 529
PsyI GACNNNGTC 1 cut(s) 186
PvuII CAGCTG 2 cut(s) 866, 1001
RsaI GTAC 2 cut(s) 330, 932
RsaNI GTAC 2 cut(s) 329, 931
RseI CAYNNNNRTG 4 cut(s) 175, 205, 559, 589
SaqAI TTAA 8 cut(s) 230, 428, 488, 504, 519, 966, 1070, 1101
SatI GCNGC 7 cut(s) 219, 252, 603, 867, 996, 999, 1010
Sau3AI GATC 5 cut(s) 449, 529, 1120, 1125, 1203
Sau96I GGNCC 2 cut(s) 80, 833
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 4 cut(s) 333, 1150, 1178, 1213
SduI GDGCHC 2 cut(s) 570, 1187
SfaNI GCATC 4 cut(s) 58, 250, 256, 640
Sfr274I CTCGAG 1 cut(s) 1019
SinI GGWCC 1 cut(s) 80
SlaI CTCGAG 1 cut(s) 1019
SmiMI CAYNNNNRTG 4 cut(s) 175, 205, 559, 589
SmlI CTYRAG 3 cut(s) 427, 1019, 1100
SmoI CTYRAG 3 cut(s) 427, 1019, 1100
SsiI CCGC 6 cut(s) 180, 218, 424, 603, 956, 1155
SspMI CTAG 1 cut(s) 471
StyD4I CCNGG 4 cut(s) 331, 1148, 1176, 1211
StyI CCWWGG 3 cut(s) 126, 557, 909
TaaI ACNGT 3 cut(s) 174, 226, 647
TaiI ACGT 3 cut(s) 642, 684, 804
TaqI TCGA 5 cut(s) 19, 783, 1020, 1113, 1206
TaqII GACCGA 1 cut(s) 836
TatI WGTACW 1 cut(s) 930
TauI GCSGC 2 cut(s) 221, 605
TfiI GAWTC 3 cut(s) 10, 770, 905
Tru1I TTAA 8 cut(s) 230, 428, 488, 504, 519, 966, 1070, 1101
Tru9I TTAA 8 cut(s) 230, 428, 488, 504, 519, 966, 1070, 1101
TscAI CASTG 2 cut(s) 64, 575
TseFI GTSAC 1 cut(s) 817
TseI GCWGC 5 cut(s) 251, 866, 995, 998, 1009
Tsp45I GTSAC 1 cut(s) 817
TspDTI ATGAA 1 cut(s) 522
TspGWI ACGGA 3 cut(s) 93, 940, 1242
TspRI CASTG 2 cut(s) 64, 575
Tth111I GACNNNGTC 1 cut(s) 186
Vha464I CTTAAG 2 cut(s) 427, 1100
VpaK11BI GGWCC 1 cut(s) 80
XagI CCTNNNNNAGG 1 cut(s) 284
XapI RAATTY 5 cut(s) 161, 386, 490, 499, 1133
XcmI CCANNNNNNNNNTGG 1 cut(s) 877
XhoI CTCGAG 1 cut(s) 1019
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.