AT2G35200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015969
Physical Location & Seq
Forward (+)
14833848 .. 14834666
819 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G35200.1

Sequence Viewer

Length: 606 bp
ATGCAACGCGAGAAAACTCAAACGAATTTGCGTTTGGACGAAGGAGGAGGCAGCGTTTGGCAGAAATGCTCAAGACACGAAGGTCGTTTTGTCTGCGTTGCCGGTGTTTGTCCTTATTGCCTCCACGAACGCCTCTCCTCTCTCTGTCCCGATTGTGCTCACGATCTTCCCTGTTCGTGTACTCCACGCGCCTCCGTGTCTTCCCGTGGAGAAGGTGACGTTTCGTTCGCCCGTATTGGGTCGGTTGGCCGTGTTGCCAACTTGATTGAATGCGAACCGGCGTTTCGGAGGTCGACATCGATTACGGGTCCTTATTTCTGGTCTGCTAAACCGGAACCGATATTAGAAACTGAGAAGGATCTTAAACCGGGTCGTGGACGTTGGCTATGGAGATTGTTCAGAGGAAACAGAGAAGAAGAGACGAAAATAAAGGCTGCTAAGGTTATGAAGAAGTCGAATTCCGTTGCCGGAGAAGTTCTATTCTCACCGACGCCGGTGACTAGTAAGGGAAATGGTTGGTACTTTCCAAGTCCGATTAAGGTTTTCAGACAATCAAGAGTCTCCAAGATGATTTTTCAACAAAGATCTCCATTGTACAGAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF740

Protein Analysis

201

Amino Acids

22.6

Weight (kDa)

9.68

Isoelectric Point (pI)

52.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015969)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32690 AT2G35200
fragaria_vesca FvH4_5g22200
malus_domestica MD06G1035300.v1.1 MD16G1273500.v1.1
prunus_persica Prupe.5G034500_v2.0.a1
pyrus_communis pycom06g02910 pycom16g24330
rosa_chinensis RchiOBHm_Chr7g0209881
rosa_laevigata RLG00000003081
rosa_multiflora Rmu_sc0003776.1_g000006
rosa_roxburghii Rroxscaffold_3G00248870
rosa_rugosa Rorug07G0116800
rosa_samantha Rh7BG245100
rosa_wichuraiana Rw7G021260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 81
AccI GTMKAC 1 cut(s) 293
AccII CGCG 2 cut(s) 9, 189
AclWI GGATC 1 cut(s) 366
AcoI YGGCCR 1 cut(s) 247
AcsI RAATTY 2 cut(s) 25, 457
AcyI GRCGYC 1 cut(s) 491
AfaI GTAC 3 cut(s) 181, 521, 596
AfiI CCNNNNNNNGG 2 cut(s) 237, 374
AgsI TTSAA 2 cut(s) 269, 578
AhlI ACTAGT 1 cut(s) 500
AjuI GAANNNNNNNTTGG 2 cut(s) 17, 49
Alw21I GWGCWC 1 cut(s) 160
Alw26I GTCTC 2 cut(s) 413, 565
AlwI GGATC 1 cut(s) 366
AoxI GGCC 1 cut(s) 247
ApeKI GCWGC 2 cut(s) 51, 434
ApoI RAATTY 2 cut(s) 25, 457
ArsI GACNNNNNNTTYG 2 cut(s) 209, 241
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 1 cut(s) 308
AsuC2I CCSGG 1 cut(s) 369
AsuHPI GGTGA 3 cut(s) 227, 477, 508
AvaII GGWCC 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 2 cut(s) 63, 421
BceAI ACGGC 1 cut(s) 234
BcnI CCSGG 1 cut(s) 369
BcoDI GTCTC 2 cut(s) 413, 565
BcuI ACTAGT 1 cut(s) 500
BfaI CTAG 1 cut(s) 501
BglII AGATCT 1 cut(s) 584
BisI GCNGC 2 cut(s) 52, 435
BlsI GCNGC 2 cut(s) 53, 436
Bme1390I CCNGG 1 cut(s) 369
Bme18I GGWCC 1 cut(s) 308
BmgT120I GGNCC 1 cut(s) 308
BmiI GGNNCC 2 cut(s) 309, 336
BmrFI CCNGG 1 cut(s) 369
BpiI GAAGAC 1 cut(s) 192
Bpu10I CCTNAGC 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 55
BpuMI CCSGG 1 cut(s) 369
Bsa29I ATCGAT 1 cut(s) 299
BsaHI GRCGYC 1 cut(s) 491
BsaJI CCNNGG 1 cut(s) 205
BsaWI WCCGGW 1 cut(s) 331
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bsc4I CCNNNNNNNGG 2 cut(s) 237, 374
Bse118I RCCGGY 3 cut(s) 101, 277, 493
BseCI ATCGAT 1 cut(s) 299
BseDI CCNNGG 1 cut(s) 205
BseLI CCNNNNNNNGG 2 cut(s) 237, 374
BseMII CTCAG 1 cut(s) 342
BseRI GAGGAG 2 cut(s) 60, 127
BseXI GCAGC 2 cut(s) 63, 421
Bsh1236I CGCG 2 cut(s) 9, 189
BshFI GGCC 1 cut(s) 249
BshVI ATCGAT 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 160
BsiSI CCGG 6 cut(s) 102, 278, 332, 368, 468, 494
BslFI GGGAC 1 cut(s) 132
BslI CCNNNNNNNGG 2 cut(s) 237, 374
BsmAI GTCTC 2 cut(s) 413, 565
BsmBI CGTCTC 1 cut(s) 413
BsmFI GGGAC 1 cut(s) 132
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 160
Bsp1407I TGTACA 1 cut(s) 594
Bsp143I GATC 3 cut(s) 163, 358, 584
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 1 cut(s) 343
BspDI ATCGAT 1 cut(s) 299
BspFNI CGCG 2 cut(s) 9, 189
BspLI GGNNCC 2 cut(s) 309, 336
BspPI GGATC 1 cut(s) 366
BsrFI RCCGGY 3 cut(s) 101, 277, 493
BsrGI TGTACA 1 cut(s) 594
BssAI RCCGGY 3 cut(s) 101, 277, 493
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 3 cut(s) 163, 358, 584
BssNI GRCGYC 1 cut(s) 491
Bst6I CTCTTC 1 cut(s) 411
BstACI GRCGYC 1 cut(s) 491
BstAUI TGTACA 1 cut(s) 594
BstDEI CTNAG 2 cut(s) 351, 438
BstDSI CCRYGG 1 cut(s) 205
BstFNI CGCG 2 cut(s) 9, 189
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 3 cut(s) 166, 361, 587
BstMAI GTCTC 2 cut(s) 413, 565
BstMBI GATC 3 cut(s) 163, 358, 584
BstSCI CCNGG 1 cut(s) 367
BstUI CGCG 2 cut(s) 9, 189
BstV1I GCAGC 2 cut(s) 63, 421
BstV2I GAAGAC 1 cut(s) 192
BstX2I RGATCY 2 cut(s) 358, 584
BstYI RGATCY 2 cut(s) 358, 584
Bsu15I ATCGAT 1 cut(s) 299
BsuRI GGCC 1 cut(s) 249
BsuTUI ATCGAT 1 cut(s) 299
BtgI CCRYGG 1 cut(s) 205
CfoI GCGC 1 cut(s) 191
Cfr10I RCCGGY 3 cut(s) 101, 277, 493
Cfr13I GGNCC 1 cut(s) 308
ClaI ATCGAT 1 cut(s) 299
CseI GACGC 1 cut(s) 499
Csp6I GTAC 3 cut(s) 180, 520, 595
CviJI RGCY 3 cut(s) 249, 385, 434
CviKI_1 RGCY 3 cut(s) 249, 385, 434
CviQI GTAC 3 cut(s) 180, 520, 595
DdeI CTNAG 2 cut(s) 351, 438
DpnI GATC 3 cut(s) 165, 360, 586
DpnII GATC 3 cut(s) 163, 358, 584
DrdI GACNNNNNNGTC 1 cut(s) 81
DseDI GACNNNNNNGTC 1 cut(s) 81
EaeI YGGCCR 1 cut(s) 247
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
Eco47I GGWCC 1 cut(s) 308
EcoO109I RGGNCCY 1 cut(s) 308
EcoRI GAATTC 1 cut(s) 457
Esp3I CGTCTC 1 cut(s) 413
FaiI YATR 2 cut(s) 388, 446
FaqI GGGAC 1 cut(s) 132
FblI GTMKAC 1 cut(s) 293
Fnu4HI GCNGC 2 cut(s) 52, 435
Fsp4HI GCNGC 2 cut(s) 52, 435
FspBI CTAG 1 cut(s) 501
GlaI GCGC 1 cut(s) 190
GluI GCNGC 2 cut(s) 52, 435
HaeIII GGCC 1 cut(s) 249
HapII CCGG 6 cut(s) 102, 278, 332, 368, 468, 494
HgaI GACGC 1 cut(s) 499
HhaI GCGC 1 cut(s) 191
Hin1I GRCGYC 1 cut(s) 491
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HincII GTYRAC 1 cut(s) 294
HindII GTYRAC 1 cut(s) 294
HinfI GANTC 1 cut(s) 558
HpaII CCGG 6 cut(s) 102, 278, 332, 368, 468, 494
HphI GGTGA 3 cut(s) 227, 477, 508
Hpy166II GTNNAC 3 cut(s) 180, 294, 377
Hpy188I TCNGA 4 cut(s) 288, 401, 534, 548
Hpy188III TCNNGA 4 cut(s) 72, 149, 161, 555
Hpy8I GTNNAC 3 cut(s) 180, 294, 377
Hpy99I CGWCG 1 cut(s) 493
HpyAV CCTTC 4 cut(s) 35, 74, 206, 349
HpyCH4IV ACGT 2 cut(s) 219, 379
HpyCH4V TGCA 1 cut(s) 4
HpyF3I CTNAG 2 cut(s) 351, 438
HpySE526I ACGT 2 cut(s) 219, 379
Hsp92I GRCGYC 1 cut(s) 491
HspAI GCGC 1 cut(s) 189
Kzo9I GATC 3 cut(s) 163, 358, 584
LpnPI CCDG 8 cut(s) 115, 184, 291, 304, 345, 381, 481, 507
Lsp1109I GCAGC 2 cut(s) 63, 421
MaeI CTAG 1 cut(s) 501
MaeII ACGT 2 cut(s) 219, 379
MaeIII GTNAC 2 cut(s) 215, 496
MalI GATC 3 cut(s) 165, 360, 586
MboI GATC 3 cut(s) 163, 358, 584
MboII GAAGA 5 cut(s) 158, 192, 425, 428, 460
MflI RGATCY 2 cut(s) 358, 584
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 2 cut(s) 25, 457
MlyI GAGTC 1 cut(s) 567
MnlI CCTC 9 cut(s) 38, 41, 131, 143, 148, 202, 282, 395, 593
MseI TTAA 3 cut(s) 363, 537, 604
MspI CCGG 6 cut(s) 102, 278, 332, 368, 468, 494
MspR9I CCNGG 1 cut(s) 369
Mva1269I GAATGC 1 cut(s) 275
MvnI CGCG 2 cut(s) 9, 189
NciI CCSGG 1 cut(s) 369
NdeII GATC 3 cut(s) 163, 358, 584
NlaIV GGNNCC 2 cut(s) 309, 336
NmuCI GTSAC 2 cut(s) 215, 496
PctI GAATGC 1 cut(s) 275
PkrI GCNGC 2 cut(s) 53, 436
PleI GAGTC 1 cut(s) 566
PpsI GAGTC 1 cut(s) 566
PpuMI RGGWCCY 1 cut(s) 308
Psp5II RGGWCCY 1 cut(s) 308
PspN4I GGNNCC 2 cut(s) 309, 336
PspPI GGNCC 1 cut(s) 308
PspPPI RGGWCCY 1 cut(s) 308
PsuI RGATCY 2 cut(s) 358, 584
RsaI GTAC 3 cut(s) 181, 521, 596
RsaNI GTAC 3 cut(s) 180, 520, 595
SalI GTCGAC 1 cut(s) 292
SaqAI TTAA 3 cut(s) 363, 537, 604
SatI GCNGC 2 cut(s) 52, 435
Sau3AI GATC 3 cut(s) 163, 358, 584
Sau96I GGNCC 1 cut(s) 308
SchI GAGTC 1 cut(s) 567
ScrFI CCNGG 1 cut(s) 369
SduI GDGCHC 1 cut(s) 160
SetI ASST 8 cut(s) 85, 217, 222, 293, 382, 444, 543, 604
SgrAI CRCCGGYG 1 cut(s) 493
SinI GGWCC 1 cut(s) 308
SmlI CTYRAG 1 cut(s) 70
SmoI CTYRAG 1 cut(s) 70
SpeI ACTAGT 1 cut(s) 500
Sse9I AATT 2 cut(s) 25, 457
SspMI CTAG 1 cut(s) 501
StyD4I CCNGG 1 cut(s) 367
TaiI ACGT 2 cut(s) 222, 382
TaqI TCGA 3 cut(s) 293, 299, 455
TasI AATT 2 cut(s) 25, 457
TatI WGTACW 2 cut(s) 179, 594
Tru1I TTAA 3 cut(s) 363, 537, 604
Tru9I TTAA 3 cut(s) 363, 537, 604
TseFI GTSAC 2 cut(s) 215, 496
TseI GCWGC 2 cut(s) 51, 434
Tsp45I GTSAC 2 cut(s) 215, 496
TspDTI ATGAA 1 cut(s) 461
TspGWI ACGGA 2 cut(s) 184, 451
VpaK11BI GGWCC 1 cut(s) 308
XapI RAATTY 2 cut(s) 25, 457
XmiI GTMKAC 1 cut(s) 293
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.