MD06G1035300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015969
Physical Location & Seq
Reverse (-)
4443885 .. 4444598
714 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1035300.v1.1.491

Sequence Viewer

Length: 714 bp
ATGGCGGCGGTTTACGTTGACGAGGAGGAGGTCTGGAAATGCCCTAAACACCCTTCCAAGCGCCGCAAGAGTGGAATCTGCCCCGTCTGCCTCCGCGAGCGCCTCGTCGTTCTCTGCCCCGACTGCGCTAACGTCCGCCCCTGCGGTTGCTGCGCCACGACGTCGTCTTCCTCGTCTTCTTCTTCGTCCTCTCGCTTCCCCGCCGGCGACGGCGTCCGAGTCTCCAAGATTCTCGAGAGCGAGCCGTCTTTCCGGCGGTCCCGGTCGGTTGCGGTCCCTTTTCTGCGCTCCACGTCGAAGTTTCTCGGCGGCGCTTTTAGACACGCGGACTTTGACTCCGCCGGCGACAGGAGCGAGCCCCAGAGCGGCGGACGGACTAAAACACCGTCGTTTTGGTCGTCGGTATTCCGCTCGTCTCACAGGAGCAAGACAAGCGAGGTTAATAATGGAGAACACGTGACCACGGCGGAGAAGAAAGCCGAGGGCGAAATCCAAGAGGAGGAAGAAGACGCAGATGCTGCGATGAGAAGGAAGATGATGAGAAAATCGAGGTCGGTGGCCGTGCCGATGATGTCTTCAAAAGCTGGTAGCGGTGGAGGTGAGAGACCGCCGGCGAAAGGCAGGGGATGGCATTTCCCGAGCCCGATCAAGGCCTTCCGGCATTCCAAAATTTCAAAGATCGTTTCCGAGCGGTCGCCTGTGTACAGAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF740

Protein Analysis

238

Amino Acids

25.94

Weight (kDa)

9.85

Isoelectric Point (pI)

84.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015969)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32690 AT2G35200
fragaria_vesca FvH4_5g22200
malus_domestica MD06G1035300.v1.1 MD16G1273500.v1.1
prunus_persica Prupe.5G034500_v2.0.a1
pyrus_communis pycom06g02910 pycom16g24330
rosa_chinensis RchiOBHm_Chr7g0209881
rosa_laevigata RLG00000003081
rosa_multiflora Rmu_sc0003776.1_g000006
rosa_roxburghii Rroxscaffold_3G00248870
rosa_rugosa Rorug07G0116800
rosa_samantha Rh7BG245100
rosa_wichuraiana Rw7G021260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 164
AccBSI CCGCTC 3 cut(s) 366, 411, 691
AccII CGCG 2 cut(s) 96, 326
AcoI YGGCCR 1 cut(s) 558
AcsI RAATTY 1 cut(s) 669
AcvI CACGTG 1 cut(s) 457
AcyI GRCGYC 2 cut(s) 161, 213
AfaI GTAC 1 cut(s) 704
AfiI CCNNNNNNNGG 5 cut(s) 348, 365, 499, 617, 649
AflIII ACRYGT 1 cut(s) 454
AgsI TTSAA 2 cut(s) 579, 675
AjiI CACGTC 1 cut(s) 294
AluBI AGCT 1 cut(s) 584
AluI AGCT 1 cut(s) 584
Alw26I GTCTC 3 cut(s) 226, 420, 598
AlwNI CAGNNNCTG 1 cut(s) 518
Ama87I CYCGRG 2 cut(s) 233, 637
AoxI GGCC 2 cut(s) 558, 651
ApeKI GCWGC 2 cut(s) 150, 518
ApoI RAATTY 1 cut(s) 669
AspLEI GCGC 6 cut(s) 63, 102, 128, 155, 288, 314
AspS9I GGNCC 2 cut(s) 258, 274
AsuC2I CCSGG 1 cut(s) 262
AsuHPI GGTGA 1 cut(s) 611
AvaI CYCGRG 2 cut(s) 233, 637
AvaII GGWCC 2 cut(s) 258, 274
BanII GRGCYC 2 cut(s) 360, 644
BbrPI CACGTG 1 cut(s) 457
BbsI GAAGAC 4 cut(s) 159, 168, 513, 567
BbvI GCAGC 2 cut(s) 137, 505
BccI CCATC 1 cut(s) 621
BceAI ACGGC 4 cut(s) 226, 229, 480, 545
BcnI CCSGG 1 cut(s) 262
BcoDI GTCTC 3 cut(s) 226, 420, 598
BfoI RGCGCY 3 cut(s) 64, 103, 315
BisI GCNGC 6 cut(s) 6, 64, 151, 310, 367, 519
BlsI GCNGC 6 cut(s) 7, 65, 152, 311, 368, 520
Bme1390I CCNGG 1 cut(s) 262
Bme18I GGWCC 2 cut(s) 258, 274
BmeT110I CYCGRG 2 cut(s) 233, 637
BmgBI CACGTC 1 cut(s) 294
BmgT120I GGNCC 2 cut(s) 258, 274
BmiI GGNNCC 2 cut(s) 260, 276
BmrFI CCNGG 1 cut(s) 262
BmsI GCATC 1 cut(s) 505
BpiI GAAGAC 4 cut(s) 159, 168, 513, 567
BpuMI CCSGG 1 cut(s) 262
BsaAI YACGTR 1 cut(s) 457
BsaHI GRCGYC 2 cut(s) 161, 213
BsaI GGTCTC 1 cut(s) 598
BsaJI CCNNGG 2 cut(s) 462, 480
BsaXI ACNNNNNCTCC 4 cut(s) 320, 350, 441, 471
Bsc4I CCNNNNNNNGG 5 cut(s) 348, 365, 499, 617, 649
Bse118I RCCGGY 3 cut(s) 203, 341, 610
BseDI CCNNGG 2 cut(s) 462, 480
BseGI GGATG 1 cut(s) 632
BseLI CCNNNNNNNGG 5 cut(s) 348, 365, 499, 617, 649
BseRI GAGGAG 3 cut(s) 38, 41, 512
BseXI GCAGC 2 cut(s) 137, 505
Bsh1236I CGCG 2 cut(s) 96, 326
Bsh1285I CGRYCG 2 cut(s) 266, 695
BshFI GGCC 2 cut(s) 560, 653
BsiEI CGRYCG 2 cut(s) 266, 695
BsiHKCI CYCGRG 2 cut(s) 233, 637
BsiSI CCGG 6 cut(s) 204, 253, 262, 342, 611, 658
BslFI GGGAC 2 cut(s) 244, 260
BslI CCNNNNNNNGG 5 cut(s) 348, 365, 499, 617, 649
BsmAI GTCTC 3 cut(s) 226, 420, 598
BsmBI CGTCTC 1 cut(s) 420
BsmFI GGGAC 2 cut(s) 244, 260
BsmI GAATGC 1 cut(s) 661
BsnI GGCC 2 cut(s) 560, 653
Bso31I GGTCTC 1 cut(s) 598
BsoBI CYCGRG 2 cut(s) 233, 637
Bsp1286I GDGCHC 2 cut(s) 360, 644
Bsp1407I TGTACA 1 cut(s) 702
Bsp143I GATC 2 cut(s) 645, 678
BspANI GGCC 2 cut(s) 560, 653
BspFNI CGCG 2 cut(s) 96, 326
BspLI GGNNCC 2 cut(s) 260, 276
BspTNI GGTCTC 1 cut(s) 598
BsrBI CCGCTC 3 cut(s) 366, 411, 691
BsrFI RCCGGY 3 cut(s) 203, 341, 610
BsrGI TGTACA 1 cut(s) 702
BssAI RCCGGY 3 cut(s) 203, 341, 610
BssECI CCNNGG 2 cut(s) 462, 480
BssMI GATC 2 cut(s) 645, 678
BssNI GRCGYC 2 cut(s) 161, 213
Bst4CI ACNGT 1 cut(s) 387
BstACI GRCGYC 2 cut(s) 161, 213
BstAPI GCANNNNNTGC 1 cut(s) 518
BstAUI TGTACA 1 cut(s) 702
BstBAI YACGTR 1 cut(s) 457
BstC8I GCNNGC 6 cut(s) 98, 205, 242, 343, 356, 612
BstDSI CCRYGG 1 cut(s) 462
BstF5I GGATG 1 cut(s) 632
BstFNI CGCG 2 cut(s) 96, 326
BstH2I RGCGCY 3 cut(s) 64, 103, 315
BstHHI GCGC 6 cut(s) 63, 102, 128, 155, 288, 314
BstKTI GATC 2 cut(s) 648, 681
BstMAI GTCTC 3 cut(s) 226, 420, 598
BstMBI GATC 2 cut(s) 645, 678
BstMCI CGRYCG 2 cut(s) 266, 695
BstMWI GCNNNNNNNGC 6 cut(s) 87, 123, 150, 351, 432, 518
BstSCI CCNGG 1 cut(s) 260
BstUI CGCG 2 cut(s) 96, 326
BstV1I GCAGC 2 cut(s) 137, 505
BstV2I GAAGAC 4 cut(s) 159, 168, 513, 567
BsuRI GGCC 2 cut(s) 560, 653
BtgI CCRYGG 1 cut(s) 462
BtgZI GCGATG 1 cut(s) 536
BtrI CACGTC 1 cut(s) 294
BtsCI GGATG 1 cut(s) 632
Cac8I GCNNGC 6 cut(s) 98, 205, 242, 343, 356, 612
CaiI CAGNNNCTG 1 cut(s) 518
CfoI GCGC 6 cut(s) 63, 102, 128, 155, 288, 314
Cfr10I RCCGGY 3 cut(s) 203, 341, 610
Cfr13I GGNCC 2 cut(s) 258, 274
CseI GACGC 2 cut(s) 202, 518
Csp6I GTAC 1 cut(s) 703
CviJI RGCY 7 cut(s) 244, 358, 479, 560, 584, 642, 653
CviKI_1 RGCY 7 cut(s) 244, 358, 479, 560, 584, 642, 653
CviQI GTAC 1 cut(s) 703
DpnI GATC 2 cut(s) 647, 680
DpnII GATC 2 cut(s) 645, 678
EaeI YGGCCR 1 cut(s) 558
EciI GGCGGA 4 cut(s) 125, 328, 384, 482
Eco147I AGGCCT 1 cut(s) 653
Eco24I GRGCYC 2 cut(s) 360, 644
Eco31I GGTCTC 1 cut(s) 598
Eco47I GGWCC 2 cut(s) 258, 274
Eco72I CACGTG 1 cut(s) 457
Eco88I CYCGRG 2 cut(s) 233, 637
EcoT38I GRGCYC 2 cut(s) 360, 644
Esp3I CGTCTC 1 cut(s) 420
FaqI GGGAC 2 cut(s) 244, 260
FauI CCCGC 1 cut(s) 208
Fnu4HI GCNGC 6 cut(s) 6, 64, 151, 310, 367, 519
FokI GGATG 1 cut(s) 639
FriOI GRGCYC 2 cut(s) 360, 644
Fsp4HI GCNGC 6 cut(s) 6, 64, 151, 310, 367, 519
GlaI GCGC 6 cut(s) 62, 101, 127, 154, 287, 313
GluI GCNGC 6 cut(s) 6, 64, 151, 310, 367, 519
HaeII RGCGCY 3 cut(s) 64, 103, 315
HaeIII GGCC 2 cut(s) 560, 653
HapII CCGG 6 cut(s) 204, 253, 262, 342, 611, 658
HgaI GACGC 2 cut(s) 202, 518
HhaI GCGC 6 cut(s) 63, 102, 128, 155, 288, 314
Hin1I GRCGYC 2 cut(s) 161, 213
Hin6I GCGC 6 cut(s) 61, 100, 126, 153, 286, 312
HinP1I GCGC 6 cut(s) 61, 100, 126, 153, 286, 312
HincII GTYRAC 1 cut(s) 19
HindII GTYRAC 1 cut(s) 19
HinfI GANTC 4 cut(s) 75, 219, 229, 335
HpaII CCGG 6 cut(s) 204, 253, 262, 342, 611, 658
HphI GGTGA 1 cut(s) 611
Hpy166II GTNNAC 3 cut(s) 13, 19, 703
Hpy188I TCNGA 2 cut(s) 218, 688
Hpy188III TCNNGA 4 cut(s) 34, 233, 235, 637
Hpy8I GTNNAC 3 cut(s) 13, 19, 703
Hpy99I CGWCG 7 cut(s) 110, 163, 166, 212, 298, 391, 403
HpyAV CCTTC 3 cut(s) 63, 522, 664
HpyCH4III ACNGT 1 cut(s) 387
HpyCH4IV ACGT 5 cut(s) 15, 132, 161, 293, 456
HpyF10VI GCNNNNNNNGC 6 cut(s) 87, 123, 150, 351, 432, 518
HpySE526I ACGT 5 cut(s) 15, 132, 161, 293, 456
Hsp92I GRCGYC 2 cut(s) 161, 213
HspAI GCGC 6 cut(s) 61, 100, 126, 153, 286, 312
KroI GCCGGC 3 cut(s) 203, 341, 610
KroNI GCCGGC 3 cut(s) 205, 343, 612
Kzo9I GATC 2 cut(s) 645, 678
LmnI GCTCC 3 cut(s) 293, 351, 423
Lsp1109I GCAGC 2 cut(s) 137, 505
LweI GCATC 1 cut(s) 505
MaeII ACGT 5 cut(s) 15, 132, 161, 293, 456
MaeIII GTNAC 1 cut(s) 457
MalI GATC 2 cut(s) 647, 680
MbiI CCGCTC 3 cut(s) 366, 411, 691
MboI GATC 2 cut(s) 645, 678
MboII GAAGA 9 cut(s) 159, 168, 171, 174, 484, 515, 518, 544, 567
MhlI GDGCHC 2 cut(s) 360, 644
MluCI AATT 1 cut(s) 669
MlyI GAGTC 2 cut(s) 228, 329
MreI CGCCGGCG 3 cut(s) 203, 341, 610
MroNI GCCGGC 3 cut(s) 203, 341, 610
MseI TTAA 2 cut(s) 441, 712
MspI CCGG 6 cut(s) 204, 253, 262, 342, 611, 658
MspR9I CCNGG 1 cut(s) 262
Mva1269I GAATGC 1 cut(s) 661
MvnI CGCG 2 cut(s) 96, 326
MwoI GCNNNNNNNGC 6 cut(s) 87, 123, 150, 351, 432, 518
NaeI GCCGGC 3 cut(s) 205, 343, 612
NciI CCSGG 1 cut(s) 262
NdeII GATC 2 cut(s) 645, 678
NgoMIV GCCGGC 3 cut(s) 203, 341, 610
NlaIV GGNNCC 2 cut(s) 260, 276
NmeAIII GCCGAG 2 cut(s) 285, 505
NmuCI GTSAC 1 cut(s) 457
PaeR7I CTCGAG 1 cut(s) 233
PceI AGGCCT 1 cut(s) 653
PcsI WCGNNNNNNNCGW 2 cut(s) 170, 395
PctI GAATGC 1 cut(s) 661
PdiI GCCGGC 3 cut(s) 205, 343, 612
PfeI GAWTC 2 cut(s) 75, 229
PflFI GACNNNGTC 2 cut(s) 163, 212
PkrI GCNGC 6 cut(s) 7, 65, 152, 311, 368, 520
PleI GAGTC 2 cut(s) 227, 329
PmaCI CACGTG 1 cut(s) 457
PmlI CACGTG 1 cut(s) 457
PpsI GAGTC 2 cut(s) 227, 329
Ppu21I YACGTR 1 cut(s) 457
PspCI CACGTG 1 cut(s) 457
PspN4I GGNNCC 2 cut(s) 260, 276
PspPI GGNCC 2 cut(s) 258, 274
PstNI CAGNNNCTG 1 cut(s) 518
PsyI GACNNNGTC 2 cut(s) 163, 212
RsaI GTAC 1 cut(s) 704
RsaNI GTAC 1 cut(s) 703
SaqAI TTAA 2 cut(s) 441, 712
SatI GCNGC 6 cut(s) 6, 64, 151, 310, 367, 519
Sau3AI GATC 2 cut(s) 645, 678
Sau96I GGNCC 2 cut(s) 258, 274
SchI GAGTC 2 cut(s) 228, 329
ScrFI CCNGG 1 cut(s) 262
SduI GDGCHC 2 cut(s) 360, 644
SfaNI GCATC 1 cut(s) 505
Sfr274I CTCGAG 1 cut(s) 233
SgrAI CRCCGGYG 3 cut(s) 203, 341, 610
SinI GGWCC 2 cut(s) 258, 274
SlaI CTCGAG 1 cut(s) 233
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
Sse9I AATT 1 cut(s) 669
SseBI AGGCCT 1 cut(s) 653
StuI AGGCCT 1 cut(s) 653
StyD4I CCNGG 1 cut(s) 260
TaaI ACNGT 1 cut(s) 387
TaiI ACGT 5 cut(s) 18, 135, 164, 296, 459
TaqI TCGA 3 cut(s) 234, 296, 548
TasI AATT 1 cut(s) 669
TatI WGTACW 1 cut(s) 702
TauI GCSGC 4 cut(s) 8, 66, 312, 369
TfiI GAWTC 2 cut(s) 75, 229
Tru1I TTAA 2 cut(s) 441, 712
Tru9I TTAA 2 cut(s) 441, 712
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 2 cut(s) 150, 518
Tsp45I GTSAC 1 cut(s) 457
TspGWI ACGGA 1 cut(s) 388
Tth111I GACNNNGTC 2 cut(s) 163, 212
VpaK11BI GGWCC 2 cut(s) 258, 274
XapI RAATTY 1 cut(s) 669
XhoI CTCGAG 1 cut(s) 233
ZraI GACGTC 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.