MD16G1273500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015969
Physical Location & Seq
Forward (+)
36329083 .. 36329796
714 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1273500.v1.1.491

Sequence Viewer

Length: 714 bp
ATGGAGGCGGTTTACGTCGACGAGGAGGAGGTCTGGAAATGTCCAAAACACCCTTCCAAACGCCGCAAAAGCGGAATCTGCCACGTCTGCCTCCGCGAGCGCCTCGTCATTCTCTGCCCCGACTGCGCTAACGTCCGCCCCTGCGGTTGCTGCGCTACGACGTCGTCTTCCTCGTCTTCGTCTTCGTCCTTTCGCTTCCCCGCCGGCGATGGCGTCCGAGTCTCGAAGCTTCTCGAGAGCGAGCCCTCGTTCCGGCGGTCCCGATCGGTAGCGGCCCCATTTCTGCGCTCCACCTCGAAGTTTTTCGGCGGTGCTTTCAGACACACAGACTTTGACTTTGTCAGCGACCGGAGCGAACCCCAAAGCGTCGGACGGACTAAAACACCGTCGTTTTGGTCGTCGGTATTCCGGTCGTCCCGCAGGAGCAAGACAAGCGAGGTTAATAATGGAGAAGACGAGACGACGGCGGAGAAGAAAGTGGAGAGCGAAATTCAAGAGGAGGAAGAAGACGCAGATGCTGCGATGAGGAGGACGATGATGAGGAAATCGAGGTCGGTGGCCGTACCGATGATGTCATCAAAATCCGGTGGCGTCGGAGGTGAGAGACCGCCGGCGAAAGGCAGGGGACGGTATTTCCCGAGCCCGATCAAGGCCTTCCGGCACTCCAAAATTTCAAAGATCTTTTCCGAGCGGTCGCCTGTGTACAGAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF740

Protein Analysis

238

Amino Acids

26.44

Weight (kDa)

9.69

Isoelectric Point (pI)

83.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015969)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32690 AT2G35200
fragaria_vesca FvH4_5g22200
malus_domestica MD06G1035300.v1.1 MD16G1273500.v1.1
prunus_persica Prupe.5G034500_v2.0.a1
pyrus_communis pycom06g02910 pycom16g24330
rosa_chinensis RchiOBHm_Chr7g0209881
rosa_laevigata RLG00000003081
rosa_multiflora Rmu_sc0003776.1_g000006
rosa_roxburghii Rroxscaffold_3G00248870
rosa_rugosa Rorug07G0116800
rosa_samantha Rh7BG245100
rosa_wichuraiana Rw7G021260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 164
AccBSI CCGCTC 1 cut(s) 691
AccI GTMKAC 1 cut(s) 18
AccII CGCG 1 cut(s) 96
AcoI YGGCCR 1 cut(s) 558
AcsI RAATTY 2 cut(s) 489, 669
AcyI GRCGYC 3 cut(s) 161, 213, 591
AfaI GTAC 2 cut(s) 564, 704
AfiI CCNNNNNNNGG 3 cut(s) 252, 617, 649
AgsI TTSAA 2 cut(s) 494, 675
AjiI CACGTC 1 cut(s) 85
AjuI GAANNNNNNNTTGG 2 cut(s) 37, 69
AluBI AGCT 1 cut(s) 229
AluI AGCT 1 cut(s) 229
Alw26I GTCTC 3 cut(s) 226, 452, 598
AlwNI CAGNNNCTG 1 cut(s) 518
Ama87I CYCGRG 2 cut(s) 233, 637
AoxI GGCC 3 cut(s) 273, 558, 651
ApeKI GCWGC 2 cut(s) 150, 518
ApoI RAATTY 2 cut(s) 489, 669
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
Asp700I GAANNNNTTC 1 cut(s) 302
AspLEI GCGC 4 cut(s) 102, 128, 155, 288
AspS9I GGNCC 2 cut(s) 258, 274
AsuHPI GGTGA 1 cut(s) 611
AvaI CYCGRG 2 cut(s) 233, 637
AvaII GGWCC 1 cut(s) 258
BanII GRGCYC 2 cut(s) 246, 644
BbsI GAAGAC 5 cut(s) 159, 168, 174, 459, 513
BbvI GCAGC 2 cut(s) 137, 505
BccI CCATC 1 cut(s) 203
BceAI ACGGC 2 cut(s) 480, 545
BcoDI GTCTC 3 cut(s) 226, 452, 598
BfoI RGCGCY 1 cut(s) 103
BglII AGATCT 1 cut(s) 678
BisI GCNGC 4 cut(s) 64, 151, 273, 519
BlsI GCNGC 4 cut(s) 65, 152, 274, 520
Bme18I GGWCC 1 cut(s) 258
BmeT110I CYCGRG 2 cut(s) 233, 637
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 2 cut(s) 258, 274
BmiI GGNNCC 2 cut(s) 260, 276
BmsI GCATC 1 cut(s) 505
BpiI GAAGAC 5 cut(s) 159, 168, 174, 459, 513
BsaHI GRCGYC 3 cut(s) 161, 213, 591
BsaI GGTCTC 1 cut(s) 598
BsaWI WCCGGW 3 cut(s) 348, 408, 584
Bsc4I CCNNNNNNNGG 3 cut(s) 252, 617, 649
Bse118I RCCGGY 2 cut(s) 203, 610
BseLI CCNNNNNNNGG 3 cut(s) 252, 617, 649
BseRI GAGGAG 4 cut(s) 38, 41, 512, 541
BseXI GCAGC 2 cut(s) 137, 505
Bsh1236I CGCG 1 cut(s) 96
Bsh1285I CGRYCG 4 cut(s) 266, 349, 413, 695
BshFI GGCC 3 cut(s) 275, 560, 653
BsiEI CGRYCG 4 cut(s) 266, 349, 413, 695
BsiHKCI CYCGRG 2 cut(s) 233, 637
BsiSI CCGG 7 cut(s) 204, 253, 349, 409, 585, 611, 658
BslFI GGGAC 3 cut(s) 244, 400, 639
BslI CCNNNNNNNGG 3 cut(s) 252, 617, 649
BsmAI GTCTC 3 cut(s) 226, 452, 598
BsmBI CGTCTC 1 cut(s) 452
BsmFI GGGAC 3 cut(s) 244, 400, 639
BsnI GGCC 3 cut(s) 275, 560, 653
Bso31I GGTCTC 1 cut(s) 598
BsoBI CYCGRG 2 cut(s) 233, 637
Bsp1286I GDGCHC 2 cut(s) 246, 644
Bsp1407I TGTACA 1 cut(s) 702
Bsp143I GATC 3 cut(s) 263, 645, 678
BspANI GGCC 3 cut(s) 275, 560, 653
BspFNI CGCG 1 cut(s) 96
BspLI GGNNCC 2 cut(s) 260, 276
BspTNI GGTCTC 1 cut(s) 598
BsrBI CCGCTC 1 cut(s) 691
BsrFI RCCGGY 2 cut(s) 203, 610
BsrGI TGTACA 1 cut(s) 702
BssAI RCCGGY 2 cut(s) 203, 610
BssMI GATC 3 cut(s) 263, 645, 678
BssNI GRCGYC 3 cut(s) 161, 213, 591
Bst4CI ACNGT 2 cut(s) 387, 630
BstACI GRCGYC 3 cut(s) 161, 213, 591
BstAPI GCANNNNNTGC 1 cut(s) 518
BstAUI TGTACA 1 cut(s) 702
BstC8I GCNNGC 4 cut(s) 98, 205, 242, 612
BstFNI CGCG 1 cut(s) 96
BstH2I RGCGCY 1 cut(s) 103
BstHHI GCGC 4 cut(s) 102, 128, 155, 288
BstKTI GATC 3 cut(s) 266, 648, 681
BstMAI GTCTC 3 cut(s) 226, 452, 598
BstMBI GATC 3 cut(s) 263, 645, 678
BstMCI CGRYCG 4 cut(s) 266, 349, 413, 695
BstMWI GCNNNNNNNGC 8 cut(s) 69, 78, 87, 123, 150, 351, 432, 518
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 2 cut(s) 137, 505
BstV2I GAAGAC 5 cut(s) 159, 168, 174, 459, 513
BstX2I RGATCY 1 cut(s) 678
BstYI RGATCY 1 cut(s) 678
BsuRI GGCC 3 cut(s) 275, 560, 653
BtgZI GCGATG 2 cut(s) 222, 536
BtrI CACGTC 1 cut(s) 85
Cac8I GCNNGC 4 cut(s) 98, 205, 242, 612
CaiI CAGNNNCTG 1 cut(s) 518
CfoI GCGC 4 cut(s) 102, 128, 155, 288
Cfr10I RCCGGY 2 cut(s) 203, 610
Cfr13I GGNCC 2 cut(s) 258, 274
CseI GACGC 4 cut(s) 202, 355, 518, 580
Csp6I GTAC 2 cut(s) 563, 703
CviJI RGCY 6 cut(s) 229, 244, 275, 560, 642, 653
CviKI_1 RGCY 6 cut(s) 229, 244, 275, 560, 642, 653
CviQI GTAC 2 cut(s) 563, 703
DpnI GATC 3 cut(s) 265, 647, 680
DpnII GATC 3 cut(s) 263, 645, 678
EaeI YGGCCR 1 cut(s) 558
EciI GGCGGA 2 cut(s) 125, 482
Eco147I AGGCCT 1 cut(s) 653
Eco24I GRGCYC 2 cut(s) 246, 644
Eco31I GGTCTC 1 cut(s) 598
Eco47I GGWCC 1 cut(s) 258
Eco88I CYCGRG 2 cut(s) 233, 637
EcoT38I GRGCYC 2 cut(s) 246, 644
Esp3I CGTCTC 1 cut(s) 452
FaqI GGGAC 3 cut(s) 244, 400, 639
FauI CCCGC 2 cut(s) 208, 425
FblI GTMKAC 1 cut(s) 18
Fnu4HI GCNGC 4 cut(s) 64, 151, 273, 519
FriOI GRGCYC 2 cut(s) 246, 644
Fsp4HI GCNGC 4 cut(s) 64, 151, 273, 519
GlaI GCGC 4 cut(s) 101, 127, 154, 287
GluI GCNGC 4 cut(s) 64, 151, 273, 519
HaeII RGCGCY 1 cut(s) 103
HaeIII GGCC 3 cut(s) 275, 560, 653
HapII CCGG 7 cut(s) 204, 253, 349, 409, 585, 611, 658
HgaI GACGC 4 cut(s) 202, 355, 518, 580
HhaI GCGC 4 cut(s) 102, 128, 155, 288
Hin1I GRCGYC 3 cut(s) 161, 213, 591
Hin6I GCGC 4 cut(s) 100, 126, 153, 286
HinP1I GCGC 4 cut(s) 100, 126, 153, 286
HincII GTYRAC 1 cut(s) 19
HindII GTYRAC 1 cut(s) 19
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 2 cut(s) 75, 219
HpaII CCGG 7 cut(s) 204, 253, 349, 409, 585, 611, 658
HphI GGTGA 1 cut(s) 611
Hpy166II GTNNAC 3 cut(s) 13, 19, 703
Hpy188I TCNGA 5 cut(s) 218, 320, 371, 596, 688
Hpy188III TCNNGA 7 cut(s) 34, 223, 233, 235, 261, 494, 637
Hpy8I GTNNAC 3 cut(s) 13, 19, 703
Hpy99I CGWCG 9 cut(s) 20, 23, 163, 166, 371, 391, 403, 466, 596
HpyAV CCTTC 2 cut(s) 63, 664
HpyCH4III ACNGT 2 cut(s) 387, 630
HpyCH4IV ACGT 4 cut(s) 15, 84, 132, 161
HpyF10VI GCNNNNNNNGC 8 cut(s) 69, 78, 87, 123, 150, 351, 432, 518
HpySE526I ACGT 4 cut(s) 15, 84, 132, 161
Hsp92I GRCGYC 3 cut(s) 161, 213, 591
HspAI GCGC 4 cut(s) 100, 126, 153, 286
KroI GCCGGC 2 cut(s) 203, 610
KroNI GCCGGC 2 cut(s) 205, 612
Kzo9I GATC 3 cut(s) 263, 645, 678
LmnI GCTCC 3 cut(s) 293, 351, 423
Lsp1109I GCAGC 2 cut(s) 137, 505
LweI GCATC 1 cut(s) 505
MaeII ACGT 4 cut(s) 15, 84, 132, 161
MalI GATC 3 cut(s) 265, 647, 680
MbiI CCGCTC 1 cut(s) 691
MboI GATC 3 cut(s) 263, 645, 678
MboII GAAGA 7 cut(s) 159, 168, 174, 464, 484, 515, 518
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 2 cut(s) 246, 644
MluCI AATT 2 cut(s) 489, 669
MlyI GAGTC 1 cut(s) 228
MmeI TCCRAC 2 cut(s) 349, 574
MreI CGCCGGCG 2 cut(s) 203, 610
MroNI GCCGGC 2 cut(s) 203, 610
MroXI GAANNNNTTC 1 cut(s) 302
MseI TTAA 2 cut(s) 441, 712
MspI CCGG 7 cut(s) 204, 253, 349, 409, 585, 611, 658
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 8 cut(s) 69, 78, 87, 123, 150, 351, 432, 518
NaeI GCCGGC 2 cut(s) 205, 612
NdeII GATC 3 cut(s) 263, 645, 678
NgoMIV GCCGGC 2 cut(s) 203, 610
NlaIV GGNNCC 2 cut(s) 260, 276
PaeR7I CTCGAG 1 cut(s) 233
PceI AGGCCT 1 cut(s) 653
PcsI WCGNNNNNNNCGW 2 cut(s) 170, 395
PdiI GCCGGC 2 cut(s) 205, 612
PdmI GAANNNNTTC 1 cut(s) 302
PfeI GAWTC 1 cut(s) 75
PflFI GACNNNGTC 2 cut(s) 163, 338
PkrI GCNGC 4 cut(s) 65, 152, 274, 520
Ple19I CGATCG 1 cut(s) 266
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
PspN4I GGNNCC 2 cut(s) 260, 276
PspPI GGNCC 2 cut(s) 258, 274
PstNI CAGNNNCTG 1 cut(s) 518
PsuI RGATCY 1 cut(s) 678
PsyI GACNNNGTC 2 cut(s) 163, 338
PvuI CGATCG 1 cut(s) 266
RsaI GTAC 2 cut(s) 564, 704
RsaNI GTAC 2 cut(s) 563, 703
SalI GTCGAC 1 cut(s) 17
SaqAI TTAA 2 cut(s) 441, 712
SatI GCNGC 4 cut(s) 64, 151, 273, 519
Sau3AI GATC 3 cut(s) 263, 645, 678
Sau96I GGNCC 2 cut(s) 258, 274
SchI GAGTC 1 cut(s) 228
SduI GDGCHC 2 cut(s) 246, 644
SfaNI GCATC 1 cut(s) 505
Sfr274I CTCGAG 1 cut(s) 233
SgrAI CRCCGGYG 2 cut(s) 203, 610
SgrDI CGTCGACG 1 cut(s) 17
SinI GGWCC 1 cut(s) 258
SlaI CTCGAG 1 cut(s) 233
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
Sse9I AATT 2 cut(s) 489, 669
SseBI AGGCCT 1 cut(s) 653
StuI AGGCCT 1 cut(s) 653
TaaI ACNGT 2 cut(s) 387, 630
TaiI ACGT 4 cut(s) 18, 87, 135, 164
TaqI TCGA 5 cut(s) 18, 224, 234, 296, 548
TasI AATT 2 cut(s) 489, 669
TatI WGTACW 1 cut(s) 702
TauI GCSGC 2 cut(s) 66, 275
TfiI GAWTC 1 cut(s) 75
Tru1I TTAA 2 cut(s) 441, 712
Tru9I TTAA 2 cut(s) 441, 712
TseI GCWGC 2 cut(s) 150, 518
TspGWI ACGGA 1 cut(s) 388
Tth111I GACNNNGTC 2 cut(s) 163, 338
VpaK11BI GGWCC 1 cut(s) 258
XapI RAATTY 2 cut(s) 489, 669
XhoI CTCGAG 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 18
XmnI GAANNNNTTC 1 cut(s) 302
ZraI GACGTC 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.