AT2G35658

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0010832
Physical Location & Seq
Reverse (-)
14990325 .. 14990935
611 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G35658.1

Sequence Viewer

Length: 276 bp
ATGCGTCGATCGGCGAGCGCCTCCATAGTTTCCGACCAACTCTCAGCGAATTCTCCTTCGCCATCACCATCTCCGCCGCGAATTAAAACCTCCGCAGATTCGGATGATAGCCACCTTCTTTTGCCTAGGTACGATCCCAACTCTCATCCCGGGAAGAAGAACAAATCGCGATTCAGATCCGCCGAGAACGCTATCCATTTCATTCCGATCGTACTCATTCTCTGCGCAGTCATACTTTGGATGTTTTCGAATCCAGTAGCAAAGATCAAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

91

Amino Acids

10.01

Weight (kDa)

10.12

Isoelectric Point (pI)

70.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35658 AT2G35658 AT2G35658 AT4G16840
fragaria_vesca FvH4_5g20070 FvH4_5g20070
malus_domestica MD04G1050500.v1.1 MD06G1049300.v1.1
prunus_persica Prupe.5G059800_v2.0.a1
pyrus_communis pycom04g04440
rosa_chinensis RchiOBHm_Chr7g0204931
rosa_multiflora Rmu_sc0004124.1_g000005
rosa_roxburghii Rroxscaffold_3G00252660
rosa_rugosa Rorug07G0086600 Rorug07G0086600
rosa_samantha Rh7AG217300 Rh7BG214200 Rh7CG230600 Rh7DG224900
rosa_wichuraiana Rw7G018850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 226
AccII CGCG 2 cut(s) 79, 169
AciI CCGC 4 cut(s) 74, 77, 93, 180
AclWI GGATC 2 cut(s) 128, 171
AcsI RAATTY 1 cut(s) 49
AfaI GTAC 2 cut(s) 131, 213
AlwI GGATC 2 cut(s) 128, 171
Ama87I CYCGRG 1 cut(s) 149
ApoI RAATTY 1 cut(s) 49
AspA2I CCTAGG 1 cut(s) 125
AspLEI GCGC 2 cut(s) 20, 227
AsuC2I CCSGG 2 cut(s) 150, 151
AsuHPI GGTGA 1 cut(s) 57
AsuII TTCGAA 1 cut(s) 248
AvaI CYCGRG 1 cut(s) 149
AvrII CCTAGG 1 cut(s) 125
BccI CCATC 2 cut(s) 70, 76
BcnI CCSGG 2 cut(s) 150, 151
BfaI CTAG 1 cut(s) 126
BfoI RGCGCY 1 cut(s) 21
BisI GCNGC 1 cut(s) 77
BlnI CCTAGG 1 cut(s) 125
BlsI GCNGC 1 cut(s) 78
Bme1390I CCNGG 2 cut(s) 150, 151
BmeT110I CYCGRG 1 cut(s) 149
BmrFI CCNGG 2 cut(s) 150, 151
Bpu14I TTCGAA 1 cut(s) 248
BpuMI CCSGG 2 cut(s) 150, 151
BsaBI GATNNNNATC 1 cut(s) 175
BsaJI CCNNGG 2 cut(s) 125, 149
Bse1I ACTGG 1 cut(s) 254
Bse8I GATNNNNATC 1 cut(s) 175
BseDI CCNNGG 2 cut(s) 125, 149
BseGI GGATG 3 cut(s) 109, 145, 246
BseJI GATNNNNATC 1 cut(s) 175
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 1 cut(s) 254
Bsh1236I CGCG 2 cut(s) 79, 169
Bsh1285I CGRYCG 2 cut(s) 11, 210
BsiEI CGRYCG 2 cut(s) 11, 210
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 1 cut(s) 150
BsoBI CYCGRG 1 cut(s) 149
Bsp119I TTCGAA 1 cut(s) 248
Bsp143I GATC 5 cut(s) 8, 133, 176, 207, 264
Bsp68I TCGCGA 1 cut(s) 169
BspACI CCGC 4 cut(s) 74, 77, 93, 180
BspCNI CTCAG 1 cut(s) 56
BspFNI CGCG 2 cut(s) 79, 169
BspPI GGATC 2 cut(s) 128, 171
BspT104I TTCGAA 1 cut(s) 248
BsrI ACTGG 1 cut(s) 254
BssECI CCNNGG 2 cut(s) 125, 149
BssMI GATC 5 cut(s) 8, 133, 176, 207, 264
BssT1I CCWWGG 1 cut(s) 125
Bst4CI ACNGT 1 cut(s) 273
BstBI TTCGAA 1 cut(s) 248
BstC8I GCNNGC 1 cut(s) 16
BstDEI CTNAG 1 cut(s) 43
BstF5I GGATG 3 cut(s) 109, 145, 246
BstFNI CGCG 2 cut(s) 79, 169
BstH2I RGCGCY 1 cut(s) 21
BstHHI GCGC 2 cut(s) 20, 227
BstKTI GATC 5 cut(s) 11, 136, 179, 210, 267
BstMBI GATC 5 cut(s) 8, 133, 176, 207, 264
BstMCI CGRYCG 2 cut(s) 11, 210
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstSCI CCNGG 2 cut(s) 148, 149
BstUI CGCG 2 cut(s) 79, 169
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
BtsCI GGATG 3 cut(s) 109, 145, 246
BtuMI TCGCGA 1 cut(s) 169
Cac8I GCNNGC 1 cut(s) 16
CfoI GCGC 2 cut(s) 20, 227
Cfr9I CCCGGG 1 cut(s) 149
Csp6I GTAC 2 cut(s) 130, 212
CviJI RGCY 1 cut(s) 111
CviKI_1 RGCY 1 cut(s) 111
CviQI GTAC 2 cut(s) 130, 212
DdeI CTNAG 1 cut(s) 43
DpnI GATC 5 cut(s) 10, 135, 178, 209, 266
DpnII GATC 5 cut(s) 8, 133, 176, 207, 264
EciI GGCGGA 2 cut(s) 63, 169
Eco130I CCWWGG 1 cut(s) 125
Eco88I CYCGRG 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 49
EcoT14I CCWWGG 1 cut(s) 125
ErhI CCWWGG 1 cut(s) 125
FaiI YATR 2 cut(s) 26, 233
Fnu4HI GCNGC 1 cut(s) 77
FokI GGATG 3 cut(s) 116, 132, 253
Fsp4HI GCNGC 1 cut(s) 77
FspBI CTAG 1 cut(s) 126
FspI TGCGCA 1 cut(s) 226
GlaI GCGC 2 cut(s) 19, 226
GluI GCNGC 1 cut(s) 77
HaeII RGCGCY 1 cut(s) 21
HapII CCGG 1 cut(s) 150
HhaI GCGC 2 cut(s) 20, 227
Hin6I GCGC 2 cut(s) 18, 225
HinP1I GCGC 2 cut(s) 18, 225
HinfI GANTC 3 cut(s) 98, 171, 250
HpaII CCGG 1 cut(s) 150
HphI GGTGA 1 cut(s) 57
Hpy188I TCNGA 4 cut(s) 34, 103, 176, 207
Hpy188III TCNNGA 1 cut(s) 168
Hpy99I CGWCG 1 cut(s) 9
HpyAV CCTTC 2 cut(s) 66, 125
HpyCH4III ACNGT 1 cut(s) 273
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpyF3I CTNAG 1 cut(s) 43
HspAI GCGC 2 cut(s) 18, 225
Kzo9I GATC 5 cut(s) 8, 133, 176, 207, 264
LpnPI CCDG 2 cut(s) 163, 267
MaeI CTAG 1 cut(s) 126
MalI GATC 5 cut(s) 10, 135, 178, 209, 266
MboI GATC 5 cut(s) 8, 133, 176, 207, 264
MboII GAAGA 2 cut(s) 166, 169
MflI RGATCY 1 cut(s) 176
MluCI AATT 2 cut(s) 49, 81
MmeI TCCRAC 1 cut(s) 57
MnlI CCTC 2 cut(s) 31, 100
MseI TTAA 1 cut(s) 84
MspI CCGG 1 cut(s) 150
MspR9I CCNGG 2 cut(s) 150, 151
MvnI CGCG 2 cut(s) 79, 169
MwoI GCNNNNNNNGC 1 cut(s) 188
NciI CCSGG 2 cut(s) 150, 151
NdeII GATC 5 cut(s) 8, 133, 176, 207, 264
NmeAIII GCCGAG 1 cut(s) 208
NruI TCGCGA 1 cut(s) 169
NsbI TGCGCA 1 cut(s) 226
NspV TTCGAA 1 cut(s) 248
PfeI GAWTC 3 cut(s) 98, 171, 250
PkrI GCNGC 1 cut(s) 78
Ple19I CGATCG 2 cut(s) 11, 210
PsuI RGATCY 1 cut(s) 176
PvuI CGATCG 2 cut(s) 11, 210
RruI TCGCGA 1 cut(s) 169
RsaI GTAC 2 cut(s) 131, 213
RsaNI GTAC 2 cut(s) 130, 212
SaqAI TTAA 1 cut(s) 84
SatI GCNGC 1 cut(s) 77
Sau3AI GATC 5 cut(s) 8, 133, 176, 207, 264
ScrFI CCNGG 2 cut(s) 150, 151
SetI ASST 3 cut(s) 92, 117, 131
SfuI TTCGAA 1 cut(s) 248
SgeI CNNG 9 cut(s) 27, 90, 138, 161, 162, 163, 180, 196, 266
SmaI CCCGGG 1 cut(s) 151
Sse9I AATT 2 cut(s) 49, 81
SsiI CCGC 4 cut(s) 74, 77, 93, 180
SspMI CTAG 1 cut(s) 126
StyD4I CCNGG 2 cut(s) 148, 149
StyI CCWWGG 1 cut(s) 125
TaaI ACNGT 1 cut(s) 273
TaqI TCGA 2 cut(s) 7, 248
TasI AATT 2 cut(s) 49, 81
TauI GCSGC 1 cut(s) 79
TfiI GAWTC 3 cut(s) 98, 171, 250
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TspDTI ATGAA 1 cut(s) 190
TspMI CCCGGG 1 cut(s) 149
XapI RAATTY 1 cut(s) 49
XmaI CCCGGG 1 cut(s) 149
XmaJI CCTAGG 1 cut(s) 125
XspI CTAG 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.