MD06G1049300.v1.1

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010832
Physical Location & Seq
Reverse (-)
6687909 .. 6689147
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1049300.v1.1.491

Sequence Viewer

Length: 441 bp
ATGGGAAGTAATCTAAAACCATATAAGCACGTAGCAAACTTTGTCTGCCGGGTGGGACAACTTTCAACAATTTTATGGGTCAAATTGAGTAGTTTTACCGTCTCTTTGTTGACCCCCATCCTAACATTCCAACCACAAGGAAAGATTCAGACAACTCGCAAGATGCAGAGATCCACAAGTGCTTCCAGAGTTTCCTCGGAGGAGGACTTGCTTCTGACGTACTCCTCCTCACCCGAATCTTCAACCTTCAAACCAACCACAGACGAGGGCTCAGATGGCGACCAGCACGAGCATCTGCATCTCCCCACATACGATCCCCTCTCTCATGTGGCAAAGAGAGAGCGACGCCGCATCAGGTCCGCAGAGAACGCCATCCACTTGATACCTGTCCTGCTCGTCCTCTGCGCCTTCATCCTCTGGTTCTTTTCCAACCCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

147

Amino Acids

16.51

Weight (kDa)

9.03

Isoelectric Point (pI)

56.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35658 AT2G35658 AT2G35658 AT4G16840
fragaria_vesca FvH4_5g20070 FvH4_5g20070
malus_domestica MD04G1050500.v1.1 MD06G1049300.v1.1
prunus_persica Prupe.5G059800_v2.0.a1
pyrus_communis pycom04g04440
rosa_chinensis RchiOBHm_Chr7g0204931
rosa_multiflora Rmu_sc0004124.1_g000005
rosa_roxburghii Rroxscaffold_3G00252660
rosa_rugosa Rorug07G0086600 Rorug07G0086600
rosa_samantha Rh7AG217300 Rh7BG214200 Rh7CG230600 Rh7DG224900
rosa_wichuraiana Rw7G018850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 349, 360
AclWI GGATC 2 cut(s) 165, 308
AcyI GRCGYC 1 cut(s) 346
AfaI GTAC 1 cut(s) 221
AgsI TTSAA 3 cut(s) 66, 243, 250
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 2 cut(s) 165, 308
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 1 cut(s) 357
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 1 cut(s) 222
AvaII GGWCC 1 cut(s) 357
BanII GRGCYC 1 cut(s) 272
BauI CACGAG 1 cut(s) 287
BccI CCATC 3 cut(s) 125, 269, 380
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 1 cut(s) 106
BisI GCNGC 1 cut(s) 349
BlsI GCNGC 1 cut(s) 350
Bme1390I CCNGG 1 cut(s) 50
Bme18I GGWCC 1 cut(s) 357
BmgT120I GGNCC 1 cut(s) 357
BmrFI CCNGG 1 cut(s) 50
BmsI GCATC 4 cut(s) 153, 301, 307, 360
BpuMI CCSGG 1 cut(s) 50
BsaAI YACGTR 1 cut(s) 31
BsaHI GRCGYC 1 cut(s) 346
BsaJI CCNNGG 1 cut(s) 195
BseDI CCNNGG 1 cut(s) 195
BseGI GGATG 3 cut(s) 117, 372, 411
BseMII CTCAG 1 cut(s) 285
BseRI GAGGAG 3 cut(s) 214, 215, 217
BsiSI CCGG 1 cut(s) 49
BslFI GGGAC 1 cut(s) 69
BsmAI GTCTC 1 cut(s) 106
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 1 cut(s) 69
Bsp1286I GDGCHC 1 cut(s) 272
Bsp143I GATC 2 cut(s) 170, 313
BspACI CCGC 2 cut(s) 349, 360
BspCNI CTCAG 1 cut(s) 284
BspPI GGATC 2 cut(s) 165, 308
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 2 cut(s) 170, 313
BssNI GRCGYC 1 cut(s) 346
BssSI CACGAG 1 cut(s) 287
Bst2BI CACGAG 1 cut(s) 287
Bst4CI ACNGT 1 cut(s) 100
BstACI GRCGYC 1 cut(s) 346
BstBAI YACGTR 1 cut(s) 31
BstDEI CTNAG 1 cut(s) 271
BstF5I GGATG 3 cut(s) 117, 372, 411
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 2 cut(s) 173, 316
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 2 cut(s) 170, 313
BstMWI GCNNNNNNNGC 2 cut(s) 276, 368
BstSCI CCNGG 1 cut(s) 48
BstX2I RGATCY 1 cut(s) 170
BstYI RGATCY 1 cut(s) 170
BtsCI GGATG 3 cut(s) 117, 372, 411
CfoI GCGC 1 cut(s) 407
Cfr13I GGNCC 1 cut(s) 357
CseI GACGC 1 cut(s) 354
Csp6I GTAC 1 cut(s) 220
CviAII CATG 1 cut(s) 326
CviJI RGCY 1 cut(s) 270
CviKI_1 RGCY 1 cut(s) 270
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 1 cut(s) 271
DpnI GATC 2 cut(s) 172, 315
DpnII GATC 2 cut(s) 170, 313
Eco24I GRGCYC 1 cut(s) 272
Eco47I GGWCC 1 cut(s) 357
EcoT38I GRGCYC 1 cut(s) 272
Esp3I CGTCTC 1 cut(s) 106
FaeI CATG 1 cut(s) 329
FaiI YATR 5 cut(s) 22, 24, 76, 310, 327
FaqI GGGAC 1 cut(s) 69
FatI CATG 1 cut(s) 325
Fnu4HI GCNGC 1 cut(s) 349
FokI GGATG 3 cut(s) 104, 359, 398
FriOI GRGCYC 1 cut(s) 272
Fsp4HI GCNGC 1 cut(s) 349
GlaI GCGC 1 cut(s) 406
GluI GCNGC 1 cut(s) 349
HapII CCGG 1 cut(s) 49
HgaI GACGC 1 cut(s) 354
HhaI GCGC 1 cut(s) 407
Hin1I GRCGYC 1 cut(s) 346
Hin1II CATG 1 cut(s) 329
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HinfI GANTC 2 cut(s) 145, 236
HpaII CCGG 1 cut(s) 49
HphI GGTGA 1 cut(s) 222
Hpy166II GTNNAC 1 cut(s) 111
Hpy188I TCNGA 4 cut(s) 150, 199, 216, 274
Hpy188III TCNNGA 1 cut(s) 186
Hpy8I GTNNAC 1 cut(s) 111
Hpy99I CGWCG 1 cut(s) 348
HpyAV CCTTC 2 cut(s) 256, 418
HpyCH4III ACNGT 1 cut(s) 100
HpyCH4IV ACGT 2 cut(s) 30, 218
HpyCH4V TGCA 2 cut(s) 166, 298
HpyF10VI GCNNNNNNNGC 2 cut(s) 276, 368
HpyF3I CTNAG 1 cut(s) 271
HpySE526I ACGT 2 cut(s) 30, 218
Hsp92I GRCGYC 1 cut(s) 346
Hsp92II CATG 1 cut(s) 329
HspAI GCGC 1 cut(s) 405
Kzo9I GATC 2 cut(s) 170, 313
LpnPI CCDG 7 cut(s) 62, 199, 296, 340, 399, 403, 404
LweI GCATC 4 cut(s) 153, 301, 307, 360
MaeII ACGT 2 cut(s) 30, 218
MalI GATC 2 cut(s) 172, 315
MboI GATC 2 cut(s) 170, 313
MboII GAAGA 1 cut(s) 231
MflI RGATCY 1 cut(s) 170
MhlI GDGCHC 1 cut(s) 272
MluCI AATT 2 cut(s) 69, 83
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 9 cut(s) 193, 196, 205, 235, 238, 259, 329, 410, 425
MspI CCGG 1 cut(s) 49
MspR9I CCNGG 1 cut(s) 50
MwoI GCNNNNNNNGC 2 cut(s) 276, 368
NciI CCSGG 1 cut(s) 50
NdeII GATC 2 cut(s) 170, 313
NlaIII CATG 1 cut(s) 329
PfeI GAWTC 2 cut(s) 145, 236
PkrI GCNGC 1 cut(s) 350
Ppu21I YACGTR 1 cut(s) 31
PspPI GGNCC 1 cut(s) 357
PsuI RGATCY 1 cut(s) 170
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
SatI GCNGC 1 cut(s) 349
Sau3AI GATC 2 cut(s) 170, 313
Sau96I GGNCC 1 cut(s) 357
ScrFI CCNGG 1 cut(s) 50
SduI GDGCHC 1 cut(s) 272
SetI ASST 5 cut(s) 33, 221, 248, 359, 388
SfaNI GCATC 4 cut(s) 153, 301, 307, 360
SinI GGWCC 1 cut(s) 357
Sse9I AATT 2 cut(s) 69, 83
SsiI CCGC 2 cut(s) 349, 360
StyD4I CCNGG 1 cut(s) 48
TaaI ACNGT 1 cut(s) 100
TaiI ACGT 2 cut(s) 33, 221
TasI AATT 2 cut(s) 69, 83
TauI GCSGC 1 cut(s) 351
TfiI GAWTC 2 cut(s) 145, 236
TspDTI ATGAA 1 cut(s) 400
VpaK11BI GGWCC 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.