Rroxscaffold_3G00252660

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010832
Physical Location & Seq
Reverse (-)
46732878 .. 46734538
1661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00252660.1

Sequence Viewer

Length: 339 bp
ATGCACAGATCCGCAAGTGCTCCCCGAGTCTCCGGGGACTTCCCGGTCAACTCGTCATCAACTTCACCGCAGCAACAACAGCCATCTTCAACCACCGCAACCCACGACTCAGAGCACCACCACGATCAAGATCTTCCCACATACGATCCCCTCTCTCATGCCGCCAAGAGAGAACGGTCTCGCATCAGGTCCGCAGAGAACGTCATCCACTTAATACCTCTCGTCCTCGCTCTCTGTGCTATCATCCTCTGGTTCTTTTCCAATCCAGATGGAATATACAATTACAGTGGGACACTTCCAAGCATTTTCCTTTACCCCGTAGGTTTCAGTGACCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

112

Amino Acids

12.42

Weight (kDa)

6.11

Isoelectric Point (pI)

70.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35658 AT2G35658 AT2G35658 AT4G16840
fragaria_vesca FvH4_5g20070 FvH4_5g20070
malus_domestica MD04G1050500.v1.1 MD06G1049300.v1.1
prunus_persica Prupe.5G059800_v2.0.a1
pyrus_communis pycom04g04440
rosa_chinensis RchiOBHm_Chr7g0204931
rosa_multiflora Rmu_sc0004124.1_g000005
rosa_roxburghii Rroxscaffold_3G00252660
rosa_rugosa Rorug07G0086600 Rorug07G0086600
rosa_samantha Rh7AG217300 Rh7BG214200 Rh7CG230600 Rh7DG224900
rosa_wichuraiana Rw7G018850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 44
AciI CCGC 5 cut(s) 12, 68, 96, 162, 192
AclWI GGATC 2 cut(s) 3, 140
AgsI TTSAA 1 cut(s) 90
Alw21I GWGCWC 2 cut(s) 22, 117
Alw26I GTCTC 2 cut(s) 34, 183
AlwI GGATC 2 cut(s) 3, 140
Ama87I CYCGRG 1 cut(s) 24
ApeKI GCWGC 1 cut(s) 70
AspS9I GGNCC 1 cut(s) 189
AsuC2I CCSGG 2 cut(s) 34, 44
AsuHPI GGTGA 1 cut(s) 57
AvaI CYCGRG 1 cut(s) 24
AvaII GGWCC 1 cut(s) 189
Bbv12I GWGCWC 2 cut(s) 22, 117
BbvI GCAGC 1 cut(s) 82
BccI CCATC 2 cut(s) 91, 263
BcnI CCSGG 2 cut(s) 34, 44
BcoDI GTCTC 2 cut(s) 34, 183
BglII AGATCT 1 cut(s) 130
BisI GCNGC 2 cut(s) 71, 162
BlsI GCNGC 2 cut(s) 72, 163
Bme1390I CCNGG 2 cut(s) 34, 44
Bme18I GGWCC 1 cut(s) 189
BmeT110I CYCGRG 1 cut(s) 24
BmgT120I GGNCC 1 cut(s) 189
BmrFI CCNGG 2 cut(s) 34, 44
BmsI GCATC 1 cut(s) 192
BpuMI CCSGG 2 cut(s) 34, 44
BsaBI GATNNNNATC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 33
Bse8I GATNNNNATC 1 cut(s) 129
BseDI CCNNGG 1 cut(s) 33
BseGI GGATG 2 cut(s) 204, 243
BseJI GATNNNNATC 1 cut(s) 129
BseMII CTCAG 1 cut(s) 123
BseXI GCAGC 1 cut(s) 82
BsiHKAI GWGCWC 2 cut(s) 22, 117
BsiHKCI CYCGRG 1 cut(s) 24
BsiSI CCGG 2 cut(s) 33, 44
BslFI GGGAC 2 cut(s) 50, 304
BsmAI GTCTC 2 cut(s) 34, 183
BsmFI GGGAC 2 cut(s) 50, 304
Bso31I GGTCTC 1 cut(s) 183
BsoBI CYCGRG 1 cut(s) 24
Bsp1286I GDGCHC 2 cut(s) 22, 117
Bsp143I GATC 4 cut(s) 8, 124, 130, 145
BspACI CCGC 5 cut(s) 12, 68, 96, 162, 192
BspCNI CTCAG 1 cut(s) 122
BspPI GGATC 2 cut(s) 3, 140
BspTNI GGTCTC 1 cut(s) 183
BssECI CCNNGG 1 cut(s) 33
BssMI GATC 4 cut(s) 8, 124, 130, 145
Bst4CI ACNGT 2 cut(s) 177, 287
BstDEI CTNAG 1 cut(s) 109
BstF5I GGATG 2 cut(s) 204, 243
BstKTI GATC 4 cut(s) 11, 127, 133, 148
BstMAI GTCTC 2 cut(s) 34, 183
BstMBI GATC 4 cut(s) 8, 124, 130, 145
BstMWI GCNNNNNNNGC 2 cut(s) 79, 236
BstSCI CCNGG 2 cut(s) 32, 42
BstV1I GCAGC 1 cut(s) 82
BstX2I RGATCY 2 cut(s) 8, 130
BstYI RGATCY 2 cut(s) 8, 130
BtsCI GGATG 2 cut(s) 204, 243
BtsIMutI CAGTG 2 cut(s) 292, 334
Cfr13I GGNCC 1 cut(s) 189
CspCI CAANNNNNGTGG 2 cut(s) 268, 303
CviAII CATG 1 cut(s) 158
CviJI RGCY 1 cut(s) 82
CviKI_1 RGCY 1 cut(s) 82
DdeI CTNAG 1 cut(s) 109
DpnI GATC 4 cut(s) 10, 126, 132, 147
DpnII GATC 4 cut(s) 8, 124, 130, 145
DrdI GACNNNNNNGTC 1 cut(s) 44
DseDI GACNNNNNNGTC 1 cut(s) 44
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 189
Eco88I CYCGRG 1 cut(s) 24
FaeI CATG 1 cut(s) 161
FaiI YATR 3 cut(s) 142, 159, 277
FaqI GGGAC 2 cut(s) 50, 304
FatI CATG 1 cut(s) 157
Fnu4HI GCNGC 2 cut(s) 71, 162
FokI GGATG 2 cut(s) 191, 230
Fsp4HI GCNGC 2 cut(s) 71, 162
GluI GCNGC 2 cut(s) 71, 162
HapII CCGG 2 cut(s) 33, 44
Hin1II CATG 1 cut(s) 161
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 2 cut(s) 27, 107
HpaII CCGG 2 cut(s) 33, 44
HphI GGTGA 1 cut(s) 57
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 1 cut(s) 112
Hpy188III TCNNGA 2 cut(s) 128, 266
Hpy8I GTNNAC 1 cut(s) 49
HpyCH4III ACNGT 2 cut(s) 177, 287
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 236
HpyF3I CTNAG 1 cut(s) 109
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 1 cut(s) 161
Kzo9I GATC 4 cut(s) 8, 124, 130, 145
LmnI GCTCC 1 cut(s) 25
LpnPI CCDG 5 cut(s) 46, 57, 172, 235, 279
Lsp1109I GCAGC 1 cut(s) 82
LweI GCATC 1 cut(s) 192
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 1 cut(s) 329
MalI GATC 4 cut(s) 10, 126, 132, 147
MboI GATC 4 cut(s) 8, 124, 130, 145
MboII GAAGA 2 cut(s) 78, 125
MflI RGATCY 2 cut(s) 8, 130
MhlI GDGCHC 2 cut(s) 22, 117
MluCI AATT 1 cut(s) 280
MlyI GAGTC 2 cut(s) 36, 101
MnlI CCTC 4 cut(s) 161, 228, 236, 257
MseI TTAA 1 cut(s) 212
MspI CCGG 2 cut(s) 33, 44
MspR9I CCNGG 2 cut(s) 34, 44
MwoI GCNNNNNNNGC 2 cut(s) 79, 236
NciI CCSGG 2 cut(s) 34, 44
NdeII GATC 4 cut(s) 8, 124, 130, 145
NlaIII CATG 1 cut(s) 161
NmuCI GTSAC 1 cut(s) 329
PkrI GCNGC 2 cut(s) 72, 163
PleI GAGTC 2 cut(s) 35, 101
PpsI GAGTC 2 cut(s) 35, 101
PspPI GGNCC 1 cut(s) 189
PsuI RGATCY 2 cut(s) 8, 130
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 2 cut(s) 71, 162
Sau3AI GATC 4 cut(s) 8, 124, 130, 145
Sau96I GGNCC 1 cut(s) 189
SchI GAGTC 2 cut(s) 36, 101
ScrFI CCNGG 2 cut(s) 34, 44
SduI GDGCHC 2 cut(s) 22, 117
SetI ASST 5 cut(s) 191, 204, 220, 325, 336
SfaNI GCATC 1 cut(s) 192
SinI GGWCC 1 cut(s) 189
Sse9I AATT 1 cut(s) 280
SsiI CCGC 5 cut(s) 12, 68, 96, 162, 192
StyD4I CCNGG 2 cut(s) 32, 42
TaaI ACNGT 2 cut(s) 177, 287
TaiI ACGT 1 cut(s) 204
TasI AATT 1 cut(s) 280
TauI GCSGC 1 cut(s) 164
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TscAI CASTG 2 cut(s) 292, 334
TseFI GTSAC 1 cut(s) 329
TseI GCWGC 1 cut(s) 70
Tsp45I GTSAC 1 cut(s) 329
TspRI CASTG 2 cut(s) 292, 334
VpaK11BI GGWCC 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.