Rorug07G0086600

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0010832
Physical Location & Seq
Forward (+)
6790777 .. 6793654
2878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0086600.1

Sequence Viewer

Length: 636 bp
ATGGACGCCGCCTCCATGTCTCTCGATTCCCCCCGCACTCCCCCACGCTCACGCTCGTCCCTGATGGAGGATGATGCTGAGGTGGACGCTCAGATTTTATGTCTGAGCCAGGGCTTGGGCCACATCGTCCAAACCGTTGAGCATCTCCGTCGGAGCACTGAAGCTCGCCGCCGTCGCCTCTCGGTCTCTCCAACTGCGCCACCACCACTCGCATCTCCCTTGGTTCTTGAGCTAATTGCACAGCAAGCGACGGATCTGGACAAGCATATCCTTCACCTGAAGGCTTCGCTGGACATCGGCATTCTCCAAGGAGGCGTGACACCACTCACACTTGCCCCCCTTCCTGCGGTGAGGCTGTGGACCAGTTTCTTCGCTGTTCAGTGCGACATGAATTTGCTGGGCGAGCTGCTAAGCCGTCTCAACGCTGGGACTGTTTTCGTTGCGGAGGACGGGACTCCAGTGGTTTACCAGACTGTTGGAAATATCGAAGATGTCCAGTTGCTGAAGAGGCTGAGTGACGGTGGCTTCTGCACTCCACATGCCAGCTGCCGAGATTTTCTTACCAACGCAGAGTTTGCTGCCATCCATGAGTTTGCTGCCATCCATGACTTACTCATGGATAGGTGCCAGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

211

Amino Acids

23.0

Weight (kDa)

5.4

Isoelectric Point (pI)

65.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35658 AT2G35658 AT2G35658 AT4G16840
fragaria_vesca FvH4_5g20070 FvH4_5g20070
malus_domestica MD04G1050500.v1.1 MD06G1049300.v1.1
prunus_persica Prupe.5G059800_v2.0.a1
pyrus_communis pycom04g04440
rosa_chinensis RchiOBHm_Chr7g0204931
rosa_multiflora Rmu_sc0004124.1_g000005
rosa_roxburghii Rroxscaffold_3G00252660
rosa_rugosa Rorug07G0086600 Rorug07G0086600
rosa_samantha Rh7AG217300 Rh7BG214200 Rh7CG230600 Rh7DG224900
rosa_wichuraiana Rw7G018850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 624
AccB7I CCANNNNNTGG 1 cut(s) 115
AciI CCGC 5 cut(s) 9, 34, 169, 347, 443
AclWI GGATC 1 cut(s) 261
AcsI RAATTY 1 cut(s) 391
AcuI CTGAAG 3 cut(s) 180, 299, 524
AcyI GRCGYC 1 cut(s) 6
AfiI CCNNNNNNNGG 3 cut(s) 67, 115, 346
AjnI CCWGG 1 cut(s) 108
AluBI AGCT 4 cut(s) 164, 232, 406, 546
AluI AGCT 4 cut(s) 164, 232, 406, 546
Alw21I GWGCWC 1 cut(s) 158
Alw26I GTCTC 3 cut(s) 24, 190, 422
AlwI GGATC 1 cut(s) 261
AlwNI CAGNNNCTG 1 cut(s) 502
AoxI GGCC 1 cut(s) 118
ApeKI GCWGC 4 cut(s) 406, 546, 578, 596
ApoI RAATTY 1 cut(s) 391
AspLEI GCGC 1 cut(s) 199
AspS9I GGNCC 2 cut(s) 118, 360
AsuHPI GGTGA 2 cut(s) 266, 361
AvaII GGWCC 1 cut(s) 360
BanI GGYRCC 1 cut(s) 624
Bbv12I GWGCWC 1 cut(s) 158
BbvCI CCTCAGC 1 cut(s) 78
BbvI GCAGC 4 cut(s) 393, 533, 565, 583
BccI CCATC 3 cut(s) 58, 590, 608
BceAI ACGGC 2 cut(s) 156, 399
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 1 cut(s) 110
BcoDI GTCTC 3 cut(s) 24, 190, 422
BisI GCNGC 6 cut(s) 9, 169, 407, 547, 579, 597
BlpI GCTNAGC 1 cut(s) 410
BlsI GCNGC 6 cut(s) 10, 170, 408, 548, 580, 598
Bme1390I CCNGG 1 cut(s) 110
Bme18I GGWCC 1 cut(s) 360
BmgT120I GGNCC 2 cut(s) 118, 360
BmiI GGNNCC 1 cut(s) 626
BmrFI CCNGG 1 cut(s) 110
BmsI GCATC 3 cut(s) 64, 151, 221
BpmI CTGGAG 1 cut(s) 441
Bpu10I CCTNAGC 1 cut(s) 78
Bpu1102I GCTNAGC 1 cut(s) 410
BpuEI CTTGAG 1 cut(s) 248
BsaHI GRCGYC 1 cut(s) 6
BsaI GGTCTC 1 cut(s) 190
BsaJI CCNNGG 3 cut(s) 109, 219, 307
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 115, 346
Bse1I ACTGG 3 cut(s) 363, 458, 496
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 3 cut(s) 109, 219, 307
BseGI GGATG 3 cut(s) 76, 582, 600
BseLI CCNNNNNNNGG 3 cut(s) 67, 115, 346
BseMII CTCAG 4 cut(s) 69, 95, 104, 503
BseNI ACTGG 3 cut(s) 363, 458, 496
BseXI GCAGC 4 cut(s) 393, 533, 565, 583
BseYI CCCAGC 2 cut(s) 397, 425
BsgI GTGCAG 1 cut(s) 514
BshFI GGCC 1 cut(s) 120
BshNI GGYRCC 1 cut(s) 624
BsiHKAI GWGCWC 1 cut(s) 158
BslFI GGGAC 3 cut(s) 43, 442, 466
BslI CCNNNNNNNGG 3 cut(s) 67, 115, 346
BsmAI GTCTC 3 cut(s) 24, 190, 422
BsmBI CGTCTC 1 cut(s) 422
BsmFI GGGAC 3 cut(s) 43, 442, 466
BsmI GAATGC 1 cut(s) 300
BsnI GGCC 1 cut(s) 120
Bso31I GGTCTC 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 158
Bsp143I GATC 1 cut(s) 253
Bsp1720I GCTNAGC 1 cut(s) 410
BspACI CCGC 5 cut(s) 9, 34, 169, 347, 443
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 4 cut(s) 70, 96, 103, 504
BspLI GGNNCC 1 cut(s) 626
BspPI GGATC 1 cut(s) 261
BspT107I GGYRCC 1 cut(s) 624
BspTNI GGTCTC 1 cut(s) 190
BsrI ACTGG 3 cut(s) 363, 458, 496
BssECI CCNNGG 3 cut(s) 109, 219, 307
BssMI GATC 1 cut(s) 253
BssNI GRCGYC 1 cut(s) 6
BssT1I CCWWGG 2 cut(s) 219, 307
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 4 cut(s) 136, 433, 475, 521
Bst6I CTCTTC 1 cut(s) 500
BstACI GRCGYC 1 cut(s) 6
BstAPI GCANNNNNTGC 1 cut(s) 575
BstC8I GCNNGC 5 cut(s) 166, 246, 404, 544, 629
BstDEI CTNAG 5 cut(s) 78, 90, 104, 410, 512
BstENI CCTNNNNNAGG 1 cut(s) 65
BstF5I GGATG 3 cut(s) 76, 582, 600
BstHHI GCGC 1 cut(s) 199
BstKTI GATC 1 cut(s) 256
BstMAI GTCTC 3 cut(s) 24, 190, 422
BstMBI GATC 1 cut(s) 253
BstMWI GCNNNNNNNGC 5 cut(s) 174, 245, 403, 508, 575
BstNI CCWGG 1 cut(s) 110
BstNSI RCATGY 1 cut(s) 542
BstSCI CCNGG 1 cut(s) 108
BstV1I GCAGC 4 cut(s) 393, 533, 565, 583
BstX2I RGATCY 1 cut(s) 253
BstXI CCANNNNNNTGG 1 cut(s) 476
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 1 cut(s) 120
BtsCI GGATG 3 cut(s) 76, 582, 600
BtsIMutI CAGTG 3 cut(s) 156, 386, 465
Cac8I GCNNGC 5 cut(s) 166, 246, 404, 544, 629
CaiI CAGNNNCTG 1 cut(s) 502
CfoI GCGC 1 cut(s) 199
Cfr13I GGNCC 2 cut(s) 118, 360
CseI GACGC 2 cut(s) 14, 95
CviAII CATG 6 cut(s) 16, 388, 539, 587, 605, 616
DdeI CTNAG 5 cut(s) 78, 90, 104, 410, 512
DpnI GATC 1 cut(s) 255
DpnII GATC 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 500
EarI CTCTTC 1 cut(s) 500
Eco130I CCWWGG 2 cut(s) 219, 307
Eco31I GGTCTC 1 cut(s) 190
Eco47I GGWCC 1 cut(s) 360
Eco57I CTGAAG 3 cut(s) 180, 299, 524
EcoNI CCTNNNNNAGG 1 cut(s) 65
EcoRII CCWGG 1 cut(s) 108
EcoT14I CCWWGG 2 cut(s) 219, 307
ErhI CCWWGG 2 cut(s) 219, 307
Esp3I CGTCTC 1 cut(s) 422
FaeI CATG 6 cut(s) 19, 391, 542, 590, 608, 619
FaiI YATR 8 cut(s) 17, 100, 267, 389, 540, 588, 606, 617
FaqI GGGAC 3 cut(s) 43, 442, 466
FatI CATG 6 cut(s) 15, 387, 538, 586, 604, 615
FauI CCCGC 1 cut(s) 41
Fnu4HI GCNGC 6 cut(s) 9, 169, 407, 547, 579, 597
FokI GGATG 3 cut(s) 83, 569, 587
Fsp4HI GCNGC 6 cut(s) 9, 169, 407, 547, 579, 597
GlaI GCGC 1 cut(s) 198
GluI GCNGC 6 cut(s) 9, 169, 407, 547, 579, 597
GsaI CCCAGC 2 cut(s) 401, 429
GsuI CTGGAG 1 cut(s) 441
HaeIII GGCC 1 cut(s) 120
HgaI GACGC 2 cut(s) 14, 95
HhaI GCGC 1 cut(s) 199
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 6 cut(s) 19, 391, 542, 590, 608, 619
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HinfI GANTC 2 cut(s) 26, 454
HphI GGTGA 2 cut(s) 266, 361
Hpy166II GTNNAC 3 cut(s) 85, 360, 466
Hpy188I TCNGA 3 cut(s) 93, 105, 153
Hpy188III TCNNGA 3 cut(s) 23, 227, 257
Hpy8I GTNNAC 3 cut(s) 85, 360, 466
Hpy99I CGWCG 3 cut(s) 153, 177, 253
HpyAV CCTTC 3 cut(s) 274, 281, 350
HpyCH4III ACNGT 4 cut(s) 136, 433, 475, 521
HpyCH4V TGCA 2 cut(s) 239, 531
HpyF10VI GCNNNNNNNGC 5 cut(s) 174, 245, 403, 508, 575
HpyF3I CTNAG 5 cut(s) 78, 90, 104, 410, 512
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 6 cut(s) 19, 391, 542, 590, 608, 619
HspAI GCGC 1 cut(s) 197
Kzo9I GATC 1 cut(s) 253
LmnI GCTCC 1 cut(s) 153
Lsp1109I GCAGC 4 cut(s) 393, 533, 565, 583
LweI GCATC 3 cut(s) 64, 151, 221
MaeIII GTNAC 2 cut(s) 316, 515
MalI GATC 1 cut(s) 255
MboI GATC 1 cut(s) 253
MboII GAAGA 3 cut(s) 361, 500, 517
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 1 cut(s) 158
MluCI AATT 2 cut(s) 234, 391
MlyI GAGTC 1 cut(s) 448
MmeI TCCRAC 3 cut(s) 131, 215, 457
MnlI CCTC 8 cut(s) 22, 61, 73, 188, 305, 345, 439, 501
MspA1I CMGCKG 1 cut(s) 546
MspR9I CCNGG 1 cut(s) 110
Mva1269I GAATGC 1 cut(s) 300
MvaI CCWGG 1 cut(s) 110
MwoI GCNNNNNNNGC 5 cut(s) 174, 245, 403, 508, 575
NdeII GATC 1 cut(s) 253
NlaIII CATG 6 cut(s) 19, 391, 542, 590, 608, 619
NlaIV GGNNCC 1 cut(s) 626
NmeAIII GCCGAG 1 cut(s) 575
NmuCI GTSAC 2 cut(s) 316, 515
NspI RCATGY 1 cut(s) 542
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PctI GAATGC 1 cut(s) 300
PfeI GAWTC 1 cut(s) 26
PflMI CCANNNNNTGG 1 cut(s) 115
PkrI GCNGC 6 cut(s) 10, 170, 408, 548, 580, 598
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
Psp6I CCWGG 1 cut(s) 108
PspFI CCCAGC 2 cut(s) 397, 425
PspGI CCWGG 1 cut(s) 108
PspN4I GGNNCC 1 cut(s) 626
PspPI GGNCC 2 cut(s) 118, 360
PstNI CAGNNNCTG 1 cut(s) 502
PsuI RGATCY 1 cut(s) 253
PvuII CAGCTG 1 cut(s) 546
SatI GCNGC 6 cut(s) 9, 169, 407, 547, 579, 597
Sau3AI GATC 1 cut(s) 253
Sau96I GGNCC 2 cut(s) 118, 360
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 1 cut(s) 110
SduI GDGCHC 1 cut(s) 158
SetI ASST 7 cut(s) 84, 166, 234, 279, 408, 548, 626
SfaNI GCATC 3 cut(s) 64, 151, 221
SinI GGWCC 1 cut(s) 360
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 2 cut(s) 234, 391
SsiI CCGC 5 cut(s) 9, 34, 169, 347, 443
StyD4I CCNGG 1 cut(s) 108
StyI CCWWGG 2 cut(s) 219, 307
TaaI ACNGT 4 cut(s) 136, 433, 475, 521
TaqI TCGA 2 cut(s) 24, 486
TaqII GACCGA 1 cut(s) 172
TasI AATT 2 cut(s) 234, 391
TauI GCSGC 2 cut(s) 11, 171
TfiI GAWTC 1 cut(s) 26
TscAI CASTG 3 cut(s) 163, 386, 465
TseFI GTSAC 2 cut(s) 316, 515
TseI GCWGC 4 cut(s) 406, 546, 578, 596
Tsp45I GTSAC 2 cut(s) 316, 515
TspDTI ATGAA 1 cut(s) 404
TspGWI ACGGA 2 cut(s) 137, 266
TspRI CASTG 3 cut(s) 163, 386, 465
Van91I CCANNNNNTGG 1 cut(s) 115
VpaK11BI GGWCC 1 cut(s) 360
XagI CCTNNNNNAGG 1 cut(s) 65
XapI RAATTY 1 cut(s) 391
XceI RCATGY 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.