FvH4_5g20070

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010832
Physical Location & Seq
Forward (+)
11832452 .. 11834566
2115 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20070.t2

Sequence Viewer

Length: 285 bp
ATGCACAGATCCGCGAGTGCTCCCCGAGTCTCCGGGGACTTCCCGGTCAACTCCTCATCAACTGCTTCACCGCAGCAGCAAAAGCAACAATCTTCAACCACCGCAACCCACGACTCAGAGCACCACCACCACCATGACGTGGATCTGCCCACATACGACCCCCTCTCTCATGCCGCCAAGAAAGAACGGTCTCGCATCAAGTCTGCAGAGAACGCCATCCACTTAATACCTCTCATCCTTGCTCTCTGTGCCATCATCCTCTGGTTCTTCTCCAATCCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

95

Amino Acids

10.37

Weight (kDa)

7.14

Isoelectric Point (pI)

72.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35658 AT2G35658 AT2G35658 AT4G16840
fragaria_vesca FvH4_5g20070 FvH4_5g20070
malus_domestica MD04G1050500.v1.1 MD06G1049300.v1.1
prunus_persica Prupe.5G059800_v2.0.a1
pyrus_communis pycom04g04440
rosa_chinensis RchiOBHm_Chr7g0204931
rosa_multiflora Rmu_sc0004124.1_g000005
rosa_roxburghii Rroxscaffold_3G00252660
rosa_rugosa Rorug07G0086600 Rorug07G0086600
rosa_samantha Rh7AG217300 Rh7BG214200 Rh7CG230600 Rh7DG224900
rosa_wichuraiana Rw7G018850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 44
AccB7I CCANNNNNTGG 1 cut(s) 139
AccII CGCG 1 cut(s) 14
AciI CCGC 4 cut(s) 12, 71, 102, 174
AclWI GGATC 2 cut(s) 3, 150
AfiI CCNNNNNNNGG 1 cut(s) 139
AgsI TTSAA 1 cut(s) 96
AjiI CACGTC 1 cut(s) 139
Alw21I GWGCWC 2 cut(s) 22, 123
Alw26I GTCTC 2 cut(s) 34, 195
AlwI GGATC 2 cut(s) 3, 150
Ama87I CYCGRG 1 cut(s) 24
ApeKI GCWGC 2 cut(s) 73, 76
AsuC2I CCSGG 2 cut(s) 34, 44
AsuHPI GGTGA 1 cut(s) 60
AvaI CYCGRG 1 cut(s) 24
Bbv12I GWGCWC 2 cut(s) 22, 123
BbvI GCAGC 2 cut(s) 85, 88
BccI CCATC 2 cut(s) 224, 260
BcnI CCSGG 2 cut(s) 34, 44
BcoDI GTCTC 2 cut(s) 34, 195
BfmI CTRYAG 1 cut(s) 204
BisI GCNGC 3 cut(s) 74, 77, 174
BlsI GCNGC 3 cut(s) 75, 78, 175
Bme1390I CCNGG 2 cut(s) 34, 44
BmeT110I CYCGRG 1 cut(s) 24
BmgBI CACGTC 1 cut(s) 139
BmrFI CCNGG 2 cut(s) 34, 44
BmsI GCATC 1 cut(s) 204
BpuMI CCSGG 2 cut(s) 34, 44
BsaI GGTCTC 1 cut(s) 195
BsaJI CCNNGG 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 139
BseDI CCNNGG 1 cut(s) 33
BseGI GGATG 3 cut(s) 216, 234, 255
BseLI CCNNNNNNNGG 1 cut(s) 139
BseMII CTCAG 1 cut(s) 129
BseRI GAGGAG 1 cut(s) 43
BseXI GCAGC 2 cut(s) 85, 88
Bsh1236I CGCG 1 cut(s) 14
BsiHKAI GWGCWC 2 cut(s) 22, 123
BsiHKCI CYCGRG 1 cut(s) 24
BsiSI CCGG 2 cut(s) 33, 44
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 1 cut(s) 139
BsmAI GTCTC 2 cut(s) 34, 195
BsmFI GGGAC 1 cut(s) 50
Bso31I GGTCTC 1 cut(s) 195
BsoBI CYCGRG 1 cut(s) 24
Bsp1286I GDGCHC 2 cut(s) 22, 123
Bsp143I GATC 2 cut(s) 8, 142
BspACI CCGC 4 cut(s) 12, 71, 102, 174
BspCNI CTCAG 1 cut(s) 128
BspFNI CGCG 1 cut(s) 14
BspMAI CTGCAG 1 cut(s) 208
BspPI GGATC 2 cut(s) 3, 150
BspTNI GGTCTC 1 cut(s) 195
BssECI CCNNGG 1 cut(s) 33
BssMI GATC 2 cut(s) 8, 142
Bst4CI ACNGT 1 cut(s) 189
BstDEI CTNAG 1 cut(s) 115
BstF5I GGATG 3 cut(s) 216, 234, 255
BstFNI CGCG 1 cut(s) 14
BstKTI GATC 2 cut(s) 11, 145
BstMAI GTCTC 2 cut(s) 34, 195
BstMBI GATC 2 cut(s) 8, 142
BstMWI GCNNNNNNNGC 3 cut(s) 82, 212, 248
BstSCI CCNGG 2 cut(s) 32, 42
BstSFI CTRYAG 1 cut(s) 204
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 85, 88
BstX2I RGATCY 2 cut(s) 8, 142
BstYI RGATCY 2 cut(s) 8, 142
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 3 cut(s) 216, 234, 255
CviAII CATG 3 cut(s) 134, 170, 282
DdeI CTNAG 1 cut(s) 115
DpnI GATC 2 cut(s) 10, 144
DpnII GATC 2 cut(s) 8, 142
DrdI GACNNNNNNGTC 1 cut(s) 44
DseDI GACNNNNNNGTC 1 cut(s) 44
Eco31I GGTCTC 1 cut(s) 195
Eco88I CYCGRG 1 cut(s) 24
FaeI CATG 3 cut(s) 137, 173, 285
FaiI YATR 4 cut(s) 135, 154, 171, 283
FaqI GGGAC 1 cut(s) 50
FatI CATG 3 cut(s) 133, 169, 281
Fnu4HI GCNGC 3 cut(s) 74, 77, 174
FokI GGATG 3 cut(s) 203, 221, 242
Fsp4HI GCNGC 3 cut(s) 74, 77, 174
GluI GCNGC 3 cut(s) 74, 77, 174
HapII CCGG 2 cut(s) 33, 44
Hin1II CATG 3 cut(s) 137, 173, 285
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 2 cut(s) 27, 113
HpaII CCGG 2 cut(s) 33, 44
HphI GGTGA 1 cut(s) 60
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 1 cut(s) 118
Hpy8I GTNNAC 1 cut(s) 49
HpyCH4III ACNGT 1 cut(s) 189
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 2 cut(s) 4, 206
HpyF10VI GCNNNNNNNGC 3 cut(s) 82, 212, 248
HpyF3I CTNAG 1 cut(s) 115
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 3 cut(s) 137, 173, 285
Kzo9I GATC 2 cut(s) 8, 142
LmnI GCTCC 1 cut(s) 25
LpnPI CCDG 3 cut(s) 46, 57, 247
Lsp1109I GCAGC 2 cut(s) 85, 88
LweI GCATC 1 cut(s) 204
MaeII ACGT 1 cut(s) 138
MalI GATC 2 cut(s) 10, 144
MboI GATC 2 cut(s) 8, 142
MboII GAAGA 2 cut(s) 84, 259
MflI RGATCY 2 cut(s) 8, 142
MhlI GDGCHC 2 cut(s) 22, 123
MlyI GAGTC 2 cut(s) 36, 107
MnlI CCTC 4 cut(s) 64, 173, 240, 269
MseI TTAA 1 cut(s) 224
MslI CAYNNNNRTG 1 cut(s) 132
MspI CCGG 2 cut(s) 33, 44
MspR9I CCNGG 2 cut(s) 34, 44
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 3 cut(s) 82, 212, 248
NciI CCSGG 2 cut(s) 34, 44
NdeII GATC 2 cut(s) 8, 142
NlaIII CATG 3 cut(s) 137, 173, 285
PflMI CCANNNNNTGG 1 cut(s) 139
PkrI GCNGC 3 cut(s) 75, 78, 175
PleI GAGTC 2 cut(s) 35, 107
PpsI GAGTC 2 cut(s) 35, 107
PstI CTGCAG 1 cut(s) 208
PsuI RGATCY 2 cut(s) 8, 142
RseI CAYNNNNRTG 1 cut(s) 132
SaqAI TTAA 1 cut(s) 224
SatI GCNGC 3 cut(s) 74, 77, 174
Sau3AI GATC 2 cut(s) 8, 142
SchI GAGTC 2 cut(s) 36, 107
ScrFI CCNGG 2 cut(s) 34, 44
SduI GDGCHC 2 cut(s) 22, 123
SetI ASST 2 cut(s) 141, 232
SfaNI GCATC 1 cut(s) 204
SfcI CTRYAG 1 cut(s) 204
SmiMI CAYNNNNRTG 1 cut(s) 132
SsiI CCGC 4 cut(s) 12, 71, 102, 174
StyD4I CCNGG 2 cut(s) 32, 42
TaaI ACNGT 1 cut(s) 189
TaiI ACGT 1 cut(s) 141
TauI GCSGC 1 cut(s) 176
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TseI GCWGC 2 cut(s) 73, 76
Van91I CCANNNNNTGG 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.