Rmu_sc0004124.1_g000005

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004124.1
OG0010832
Physical Location & Seq
Reverse (-)
26361 .. 26681
321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004124.1_g000005.1.cds

Sequence Viewer

Length: 321 bp
atgcacagatccgcgagtgctcctcgagtctccggggacttcccggtcaacccgtcatcaacttcaccgcagcaacaacagcaatcttcaaccaccgcgacccacgactcagagcaccaccacgatcaagatcttcccacatacgatcccctctctcatgccgccaagagagaacggtctcgcatcaagtccgcagagaacgccatccacttaatacctctcgtcctcgctctctgtgctatcatcctctggttcttctccaatccaggtacacaattacaatctttcccatcttcgtcatgtcttgcttccatggactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

106

Amino Acids

11.62

Weight (kDa)

6.35

Isoelectric Point (pI)

85.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35658 AT2G35658 AT2G35658 AT4G16840
fragaria_vesca FvH4_5g20070 FvH4_5g20070
malus_domestica MD04G1050500.v1.1 MD06G1049300.v1.1
prunus_persica Prupe.5G059800_v2.0.a1
pyrus_communis pycom04g04440
rosa_chinensis RchiOBHm_Chr7g0204931
rosa_multiflora Rmu_sc0004124.1_g000005
rosa_roxburghii Rroxscaffold_3G00252660
rosa_rugosa Rorug07G0086600 Rorug07G0086600
rosa_samantha Rh7AG217300 Rh7BG214200 Rh7CG230600 Rh7DG224900
rosa_wichuraiana Rw7G018850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 44
AccII CGCG 2 cut(s) 14, 98
AciI CCGC 5 cut(s) 12, 68, 96, 162, 192
AclWI GGATC 2 cut(s) 3, 140
AfaI GTAC 1 cut(s) 271
AgsI TTSAA 1 cut(s) 90
AjnI CCWGG 1 cut(s) 265
Alw21I GWGCWC 2 cut(s) 22, 117
Alw26I GTCTC 2 cut(s) 34, 183
AlwI GGATC 2 cut(s) 3, 140
Ama87I CYCGRG 1 cut(s) 24
ApeKI GCWGC 1 cut(s) 70
AsuC2I CCSGG 2 cut(s) 34, 44
AsuHPI GGTGA 1 cut(s) 57
AvaI CYCGRG 1 cut(s) 24
Bbv12I GWGCWC 2 cut(s) 22, 117
BbvI GCAGC 1 cut(s) 82
BccI CCATC 2 cut(s) 212, 298
BciT130I CCWGG 1 cut(s) 267
BcnI CCSGG 2 cut(s) 34, 44
BcoDI GTCTC 2 cut(s) 34, 183
BglII AGATCT 1 cut(s) 130
BisI GCNGC 2 cut(s) 71, 162
BlsI GCNGC 2 cut(s) 72, 163
Bme1390I CCNGG 3 cut(s) 34, 44, 267
BmeT110I CYCGRG 1 cut(s) 24
BmrFI CCNGG 3 cut(s) 34, 44, 267
BmsI GCATC 1 cut(s) 192
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpuMI CCSGG 2 cut(s) 34, 44
BsaBI GATNNNNATC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 2 cut(s) 33, 312
Bse8I GATNNNNATC 1 cut(s) 129
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 33, 312
BseGI GGATG 2 cut(s) 204, 243
BseJI GATNNNNATC 1 cut(s) 129
BseMII CTCAG 1 cut(s) 123
BseRI GAGGAG 1 cut(s) 12
BseXI GCAGC 1 cut(s) 82
Bsh1236I CGCG 2 cut(s) 14, 98
BsiHKAI GWGCWC 2 cut(s) 22, 117
BsiHKCI CYCGRG 1 cut(s) 24
BsiSI CCGG 2 cut(s) 33, 44
BslFI GGGAC 1 cut(s) 50
BsmAI GTCTC 2 cut(s) 34, 183
BsmFI GGGAC 1 cut(s) 50
Bso31I GGTCTC 1 cut(s) 183
BsoBI CYCGRG 1 cut(s) 24
Bsp1286I GDGCHC 2 cut(s) 22, 117
Bsp143I GATC 4 cut(s) 8, 124, 130, 145
Bsp19I CCATGG 1 cut(s) 312
BspACI CCGC 5 cut(s) 12, 68, 96, 162, 192
BspCNI CTCAG 1 cut(s) 122
BspFNI CGCG 2 cut(s) 14, 98
BspPI GGATC 2 cut(s) 3, 140
BspTNI GGTCTC 1 cut(s) 183
BssECI CCNNGG 2 cut(s) 33, 312
BssMI GATC 4 cut(s) 8, 124, 130, 145
BssT1I CCWWGG 1 cut(s) 312
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 1 cut(s) 177
BstDEI CTNAG 1 cut(s) 109
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 2 cut(s) 204, 243
BstFNI CGCG 2 cut(s) 14, 98
BstKTI GATC 4 cut(s) 11, 127, 133, 148
BstMAI GTCTC 2 cut(s) 34, 183
BstMBI GATC 4 cut(s) 8, 124, 130, 145
BstMWI GCNNNNNNNGC 3 cut(s) 79, 200, 236
BstNI CCWGG 1 cut(s) 267
BstSCI CCNGG 3 cut(s) 32, 42, 265
BstUI CGCG 2 cut(s) 14, 98
BstV1I GCAGC 1 cut(s) 82
BstX2I RGATCY 2 cut(s) 8, 130
BstYI RGATCY 2 cut(s) 8, 130
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 2 cut(s) 204, 243
Csp6I GTAC 1 cut(s) 270
CviAII CATG 3 cut(s) 158, 300, 313
CviQI GTAC 1 cut(s) 270
DdeI CTNAG 1 cut(s) 109
DpnI GATC 4 cut(s) 10, 126, 132, 147
DpnII GATC 4 cut(s) 8, 124, 130, 145
DrdI GACNNNNNNGTC 1 cut(s) 44
DseDI GACNNNNNNGTC 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 312
Eco31I GGTCTC 1 cut(s) 183
Eco88I CYCGRG 1 cut(s) 24
EcoRII CCWGG 1 cut(s) 265
EcoT14I CCWWGG 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 312
FaeI CATG 3 cut(s) 161, 303, 316
FaiI YATR 4 cut(s) 142, 159, 301, 314
FaqI GGGAC 1 cut(s) 50
FatI CATG 3 cut(s) 157, 299, 312
Fnu4HI GCNGC 2 cut(s) 71, 162
FokI GGATG 2 cut(s) 191, 230
Fsp4HI GCNGC 2 cut(s) 71, 162
GluI GCNGC 2 cut(s) 71, 162
HapII CCGG 2 cut(s) 33, 44
Hin1II CATG 3 cut(s) 161, 303, 316
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 2 cut(s) 27, 107
HpaII CCGG 2 cut(s) 33, 44
HphI GGTGA 1 cut(s) 57
Hpy166II GTNNAC 2 cut(s) 49, 272
Hpy188I TCNGA 1 cut(s) 112
Hpy188III TCNNGA 1 cut(s) 128
Hpy8I GTNNAC 2 cut(s) 49, 272
HpyCH4III ACNGT 1 cut(s) 177
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 200, 236
HpyF3I CTNAG 1 cut(s) 109
Hsp92II CATG 3 cut(s) 161, 303, 316
Kzo9I GATC 4 cut(s) 8, 124, 130, 145
LmnI GCTCC 1 cut(s) 25
LpnPI CCDG 5 cut(s) 46, 57, 235, 252, 279
Lsp1109I GCAGC 1 cut(s) 82
LweI GCATC 1 cut(s) 192
MalI GATC 4 cut(s) 10, 126, 132, 147
MboI GATC 4 cut(s) 8, 124, 130, 145
MboII GAAGA 4 cut(s) 78, 125, 247, 285
MflI RGATCY 2 cut(s) 8, 130
MhlI GDGCHC 2 cut(s) 22, 117
MluCI AATT 1 cut(s) 275
MlyI GAGTC 2 cut(s) 36, 101
MnlI CCTC 5 cut(s) 33, 161, 228, 236, 257
MseI TTAA 1 cut(s) 212
MspI CCGG 2 cut(s) 33, 44
MspR9I CCNGG 3 cut(s) 34, 44, 267
MvaI CCWGG 1 cut(s) 267
MvnI CGCG 2 cut(s) 14, 98
MwoI GCNNNNNNNGC 3 cut(s) 79, 200, 236
NciI CCSGG 2 cut(s) 34, 44
NcoI CCATGG 1 cut(s) 312
NdeII GATC 4 cut(s) 8, 124, 130, 145
NlaIII CATG 3 cut(s) 161, 303, 316
PaeR7I CTCGAG 1 cut(s) 24
PkrI GCNGC 2 cut(s) 72, 163
PleI GAGTC 2 cut(s) 35, 101
PpsI GAGTC 2 cut(s) 35, 101
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspXI VCTCGAGB 1 cut(s) 24
PsuI RGATCY 2 cut(s) 8, 130
RsaI GTAC 1 cut(s) 271
RsaNI GTAC 1 cut(s) 270
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 2 cut(s) 71, 162
Sau3AI GATC 4 cut(s) 8, 124, 130, 145
SchI GAGTC 2 cut(s) 36, 101
ScrFI CCNGG 3 cut(s) 34, 44, 267
SduI GDGCHC 2 cut(s) 22, 117
SetI ASST 2 cut(s) 220, 271
SfaNI GCATC 1 cut(s) 192
Sfr274I CTCGAG 1 cut(s) 24
SlaI CTCGAG 1 cut(s) 24
SmlI CTYRAG 1 cut(s) 24
SmoI CTYRAG 1 cut(s) 24
Sse9I AATT 1 cut(s) 275
SsiI CCGC 5 cut(s) 12, 68, 96, 162, 192
StyD4I CCNGG 3 cut(s) 32, 42, 265
StyI CCWWGG 1 cut(s) 312
TaaI ACNGT 1 cut(s) 177
TaqI TCGA 1 cut(s) 25
TasI AATT 1 cut(s) 275
TauI GCSGC 1 cut(s) 164
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TseI GCWGC 1 cut(s) 70
XhoI CTCGAG 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.