AT2G39430

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0016187
Physical Location & Seq
Forward (+)
16463347 .. 16464531
1185 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G39430.1

Sequence Viewer

Length: 969 bp
ATGGCCAAAGCTCTTCACATAACCATCTTTCTCTTCCTCATATCCTCCAATCTCCTCGCATTTATCAACTCCGCCAGACTTCTTGACGAGATCCAACCTCAGCCTCAGTTAGTCCCTACCGGCCAAATCCCCACTGTGGCTCCAACCGAAGCTGAGGAGGAGGATGGAACCGATGACAACCCAGGACTAGCAACCACAACCACAACTGCATCAGCAGTTACAGTCCCAGCCGGTCCAGCAGAGGCTACAGAGCCATTACTAGAGTTCTTTATGCATGACGTGCTAGGCGGATCTCACCCATCAGCTCGTGTGGTTACAGGTATAGTAGCTCAAACTGAAGTGAACGGAATACCATTCTCAAAGGCCAGTAACAGCATTTTCCCGGTTGACAATGGAGTCCCACTGGTCAACTCAAACAACATCAACAGTGTCATTAACCCAAACACAGCTCCACTTCTCACCGGACTTGGCGGTGCTCAAACCTCCACCGTCATCCAAAACACTAACGGAAATTCCAACGATGCCCTTAGCGCCAACAGTCTCCCTTTTGTAACTGCCGGGAACCTTCCTCCCGGTGCTGCCCTCCAGCATCTCATGTTCGGAACCATAACCGTTGTAGATGATGAGCTAACCGAAAGCCACGAGCTTGGCTCAGCCGTTATAGGGAGAGCTCAAGGCTTTTACTTGGCCAGTTCCCTGGACGGAACAAGTCAGACTCTTTCTCTGACTGTATTGCTACATGGTGAGCATGACCAGCATGACACTTTAGACGATGCCATTAGTTTCTTTGGGGTTCACAGAACCGCCTCTCATGCCTCCCAGATCGCGGTTATAGGCGGGACCGGGAAGTTTGAACACGCCAAAGGGTATGCCATAGTGGAAACTCTGCATAACCAGGACAACCAGCATATCACTGATGGTCAAGACACCATCCTCCATTTCAGTGTATACCTTACCTACAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

322

Amino Acids

33.76

Weight (kDa)

4.63

Isoelectric Point (pI)

30.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 184 - 318 7.9e-27 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39430 AT3G55230
fragaria_vesca FvH4_7g21000
malus_domestica MD01G1119900.v1.1 MD07G1189200.v1.1
prunus_persica Prupe.2G226700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365391
rosa_laevigata RLG00000027426
rosa_multiflora Rmu_sc0008527.1_g000004
rosa_roxburghii Rroxscaffold_4G00291260
rosa_rugosa Rorug01G0321600
rosa_samantha Rh1AG330500 Rh1BG292000 Rh1DG323600
rosa_wichuraiana Rw1G029300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
AccI GTMKAC 1 cut(s) 948
AccII CGCG 1 cut(s) 827
AciI CCGC 6 cut(s) 72, 288, 471, 804, 827, 837
AclWI GGATC 2 cut(s) 85, 298
AcoI YGGCCR 3 cut(s) 3, 121, 687
AcsI RAATTY 1 cut(s) 511
AcuI CTGAAG 1 cut(s) 357
AfiI CCNNNNNNNGG 3 cut(s) 136, 663, 826
AgsI TTSAA 1 cut(s) 854
AjiI CACGTC 1 cut(s) 280
AjnI CCWGG 3 cut(s) 181, 696, 894
AluBI AGCT 9 cut(s) 11, 152, 305, 329, 449, 628, 646, 671, 965
AluI AGCT 9 cut(s) 11, 152, 305, 329, 449, 628, 646, 671, 965
Alw21I GWGCWC 2 cut(s) 478, 673
Alw26I GTCTC 1 cut(s) 545
AlwI GGATC 2 cut(s) 85, 298
AoxI GGCC 4 cut(s) 3, 121, 363, 687
ApeKI GCWGC 1 cut(s) 578
ApoI RAATTY 1 cut(s) 511
AspLEI GCGC 1 cut(s) 533
AspS9I GGNCC 2 cut(s) 233, 840
AsuC2I CCSGG 4 cut(s) 383, 559, 573, 844
AsuHPI GGTGA 3 cut(s) 287, 451, 755
AvaII GGWCC 2 cut(s) 233, 840
BalI TGGCCA 2 cut(s) 5, 689
BanII GRGCYC 1 cut(s) 673
BauI CACGAG 2 cut(s) 306, 641
Bbv12I GWGCWC 2 cut(s) 478, 673
BbvCI CCTCAGC 2 cut(s) 99, 153
BbvI GCAGC 1 cut(s) 565
BccI CCATC 5 cut(s) 32, 158, 307, 911, 938
BceAI ACGGC 1 cut(s) 641
BciT130I CCWGG 3 cut(s) 183, 698, 896
BcnI CCSGG 4 cut(s) 383, 559, 573, 844
BcoDI GTCTC 1 cut(s) 545
BfaI CTAG 3 cut(s) 188, 260, 284
BfmI CTRYAG 1 cut(s) 246
BfoI RGCGCY 1 cut(s) 534
BisI GCNGC 1 cut(s) 579
BlpI GCTNAGC 1 cut(s) 652
BlsI GCNGC 1 cut(s) 580
Bme1390I CCNGG 7 cut(s) 183, 383, 559, 573, 698, 844, 896
Bme18I GGWCC 2 cut(s) 233, 840
BmgBI CACGTC 1 cut(s) 280
BmgT120I GGNCC 2 cut(s) 233, 840
BmiI GGNNCC 5 cut(s) 141, 169, 563, 604, 841
BmrFI CCNGG 7 cut(s) 183, 383, 559, 573, 698, 844, 896
BmsI GCATC 4 cut(s) 218, 511, 598, 763
BplI GAGNNNNNCTC 2 cut(s) 635, 667
BpmI CTGGAG 1 cut(s) 569
Bpu10I CCTNAGC 3 cut(s) 99, 153, 527
Bpu1102I GCTNAGC 1 cut(s) 652
BpuEI CTTGAG 1 cut(s) 657
BpuMI CCSGG 4 cut(s) 383, 559, 573, 844
BsaJI CCNNGG 2 cut(s) 181, 696
BsaWI WCCGGW 1 cut(s) 461
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 663, 826
Bse118I RCCGGY 2 cut(s) 119, 230
Bse1I ACTGG 3 cut(s) 366, 408, 690
BseBI CCWGG 3 cut(s) 183, 698, 896
BseDI CCNNGG 2 cut(s) 181, 696
BseGI GGATG 3 cut(s) 169, 492, 930
BseLI CCNNNNNNNGG 3 cut(s) 136, 663, 826
BseMII CTCAG 4 cut(s) 113, 119, 144, 666
BseNI ACTGG 3 cut(s) 366, 408, 690
BseRI GAGGAG 3 cut(s) 44, 170, 173
BseXI GCAGC 1 cut(s) 565
BseYI CCCAGC 1 cut(s) 226
Bsh1236I CGCG 1 cut(s) 827
BshFI GGCC 4 cut(s) 5, 123, 365, 689
BsiHKAI GWGCWC 2 cut(s) 478, 673
BsiSI CCGG 7 cut(s) 120, 231, 383, 462, 558, 573, 843
BslFI GGGAC 4 cut(s) 98, 209, 383, 853
BslI CCNNNNNNNGG 3 cut(s) 136, 663, 826
BsmAI GTCTC 1 cut(s) 545
BsmFI GGGAC 4 cut(s) 98, 209, 383, 853
BsnI GGCC 4 cut(s) 5, 123, 365, 689
Bsp1286I GDGCHC 2 cut(s) 478, 673
Bsp143I GATC 3 cut(s) 90, 290, 822
Bsp1720I GCTNAGC 1 cut(s) 652
BspACI CCGC 6 cut(s) 72, 288, 471, 804, 827, 837
BspANI GGCC 4 cut(s) 5, 123, 365, 689
BspCNI CTCAG 4 cut(s) 112, 118, 145, 665
BspFNI CGCG 1 cut(s) 827
BspLI GGNNCC 5 cut(s) 141, 169, 563, 604, 841
BspPI GGATC 2 cut(s) 85, 298
BspQI GCTCTTC 1 cut(s) 18
BsrFI RCCGGY 2 cut(s) 119, 230
BsrI ACTGG 3 cut(s) 366, 408, 690
BssAI RCCGGY 2 cut(s) 119, 230
BssECI CCNNGG 2 cut(s) 181, 696
BssMI GATC 3 cut(s) 90, 290, 822
BssNAI GTATAC 1 cut(s) 949
BssSI CACGAG 2 cut(s) 306, 641
Bst1107I GTATAC 1 cut(s) 949
Bst2BI CACGAG 2 cut(s) 306, 641
Bst2UI CCWGG 3 cut(s) 183, 698, 896
Bst4CI ACNGT 7 cut(s) 136, 223, 428, 490, 539, 613, 730
Bst6I CTCTTC 2 cut(s) 18, 38
BstAPI GCANNNNNTGC 1 cut(s) 280
BstDEI CTNAG 5 cut(s) 99, 105, 153, 527, 652
BstF5I GGATG 3 cut(s) 169, 492, 930
BstFNI CGCG 1 cut(s) 827
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 1 cut(s) 533
BstKTI GATC 3 cut(s) 93, 293, 825
BstMAI GTCTC 1 cut(s) 545
BstMBI GATC 3 cut(s) 90, 290, 822
BstMWI GCNNNNNNNGC 5 cut(s) 236, 280, 530, 754, 812
BstNI CCWGG 3 cut(s) 183, 698, 896
BstSCI CCNGG 7 cut(s) 181, 381, 557, 571, 696, 842, 894
BstSFI CTRYAG 1 cut(s) 246
BstUI CGCG 1 cut(s) 827
BstV1I GCAGC 1 cut(s) 565
BstX2I RGATCY 2 cut(s) 90, 290
BstXI CCANNNNNNTGG 2 cut(s) 647, 697
BstYI RGATCY 2 cut(s) 90, 290
BstZ17I GTATAC 1 cut(s) 949
BsuRI GGCC 4 cut(s) 5, 123, 365, 689
BtrI CACGTC 1 cut(s) 280
BtsCI GGATG 3 cut(s) 169, 492, 930
BtsIMutI CAGTG 5 cut(s) 132, 401, 433, 912, 949
CfoI GCGC 1 cut(s) 533
Cfr10I RCCGGY 2 cut(s) 119, 230
Cfr13I GGNCC 2 cut(s) 233, 840
CviAII CATG 6 cut(s) 275, 595, 740, 749, 758, 812
DdeI CTNAG 5 cut(s) 99, 105, 153, 527, 652
DpnI GATC 3 cut(s) 92, 292, 824
DpnII GATC 3 cut(s) 90, 290, 822
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
EaeI YGGCCR 3 cut(s) 3, 121, 687
Eam1104I CTCTTC 2 cut(s) 18, 38
EarI CTCTTC 2 cut(s) 18, 38
EciI GGCGGA 2 cut(s) 61, 303
Ecl136II GAGCTC 1 cut(s) 671
Eco24I GRGCYC 1 cut(s) 673
Eco47I GGWCC 2 cut(s) 233, 840
Eco53kI GAGCTC 1 cut(s) 671
Eco57I CTGAAG 1 cut(s) 357
EcoICRI GAGCTC 1 cut(s) 671
EcoRII CCWGG 3 cut(s) 181, 696, 894
EcoT22I ATGCAT 1 cut(s) 276
EcoT38I GRGCYC 1 cut(s) 673
FaeI CATG 6 cut(s) 278, 598, 743, 752, 761, 815
FaqI GGGAC 4 cut(s) 98, 209, 383, 853
FatI CATG 6 cut(s) 274, 594, 739, 748, 757, 811
FauI CCCGC 1 cut(s) 830
FblI GTMKAC 1 cut(s) 948
Fnu4HI GCNGC 1 cut(s) 579
FokI GGATG 3 cut(s) 176, 479, 917
FriOI GRGCYC 1 cut(s) 673
Fsp4HI GCNGC 1 cut(s) 579
FspBI CTAG 3 cut(s) 188, 260, 284
GlaI GCGC 1 cut(s) 532
GluI GCNGC 1 cut(s) 579
GsaI CCCAGC 1 cut(s) 230
GsuI CTGGAG 1 cut(s) 569
HaeII RGCGCY 1 cut(s) 534
HaeIII GGCC 4 cut(s) 5, 123, 365, 689
HapII CCGG 7 cut(s) 120, 231, 383, 462, 558, 573, 843
HhaI GCGC 1 cut(s) 533
Hin1II CATG 6 cut(s) 278, 598, 743, 752, 761, 815
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HincII GTYRAC 2 cut(s) 388, 409
HindII GTYRAC 2 cut(s) 388, 409
HindIII AAGCTT 1 cut(s) 963
HinfI GANTC 2 cut(s) 396, 715
HpaII CCGG 7 cut(s) 120, 231, 383, 462, 558, 573, 843
HphI GGTGA 3 cut(s) 287, 451, 755
Hpy166II GTNNAC 5 cut(s) 343, 388, 409, 796, 949
Hpy188I TCNGA 3 cut(s) 602, 714, 726
Hpy188III TCNNGA 2 cut(s) 83, 923
Hpy8I GTNNAC 5 cut(s) 343, 388, 409, 796, 949
HpyAV CCTTC 1 cut(s) 575
HpyCH4III ACNGT 7 cut(s) 136, 223, 428, 490, 539, 613, 730
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 3 cut(s) 209, 274, 889
HpyF10VI GCNNNNNNNGC 5 cut(s) 236, 280, 530, 754, 812
HpyF3I CTNAG 5 cut(s) 99, 105, 153, 527, 652
HpySE526I ACGT 1 cut(s) 279
Hsp92II CATG 6 cut(s) 278, 598, 743, 752, 761, 815
HspAI GCGC 1 cut(s) 531
Kzo9I GATC 3 cut(s) 90, 290, 822
LguI GCTCTTC 1 cut(s) 18
LmnI GCTCC 2 cut(s) 145, 454
Lsp1109I GCAGC 1 cut(s) 565
LweI GCATC 4 cut(s) 218, 511, 598, 763
MaeI CTAG 3 cut(s) 188, 260, 284
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 4 cut(s) 217, 313, 368, 550
MalI GATC 3 cut(s) 92, 292, 824
MboI GATC 3 cut(s) 90, 290, 822
MboII GAAGA 2 cut(s) 5, 25
MflI RGATCY 2 cut(s) 90, 290
MhlI GDGCHC 2 cut(s) 478, 673
MlsI TGGCCA 2 cut(s) 5, 689
MluCI AATT 1 cut(s) 511
MluNI TGGCCA 2 cut(s) 5, 689
MlyI GAGTC 2 cut(s) 405, 709
MmeI TCCRAC 3 cut(s) 118, 167, 540
Mox20I TGGCCA 2 cut(s) 5, 689
Mph1103I ATGCAT 1 cut(s) 276
MscI TGGCCA 2 cut(s) 5, 689
MseI TTAA 1 cut(s) 435
MslI CAYNNNNRTG 1 cut(s) 942
Msp20I TGGCCA 2 cut(s) 5, 689
MspI CCGG 7 cut(s) 120, 231, 383, 462, 558, 573, 843
MspR9I CCNGG 7 cut(s) 183, 383, 559, 573, 698, 844, 896
MvaI CCWGG 3 cut(s) 183, 698, 896
MvnI CGCG 1 cut(s) 827
MwoI GCNNNNNNNGC 5 cut(s) 236, 280, 530, 754, 812
NciI CCSGG 4 cut(s) 383, 559, 573, 844
NdeII GATC 3 cut(s) 90, 290, 822
NlaIII CATG 6 cut(s) 278, 598, 743, 752, 761, 815
NlaIV GGNNCC 5 cut(s) 141, 169, 563, 604, 841
NsiI ATGCAT 1 cut(s) 276
PciSI GCTCTTC 1 cut(s) 18
PkrI GCNGC 1 cut(s) 580
PleI GAGTC 2 cut(s) 404, 709
PpsI GAGTC 2 cut(s) 404, 709
Psp124BI GAGCTC 1 cut(s) 673
Psp6I CCWGG 3 cut(s) 181, 696, 894
PspFI CCCAGC 1 cut(s) 226
PspGI CCWGG 3 cut(s) 181, 696, 894
PspN4I GGNNCC 5 cut(s) 141, 169, 563, 604, 841
PspPI GGNCC 2 cut(s) 233, 840
PsuI RGATCY 2 cut(s) 90, 290
RseI CAYNNNNRTG 1 cut(s) 942
SacI GAGCTC 1 cut(s) 673
SapI GCTCTTC 1 cut(s) 18
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 1 cut(s) 579
Sau3AI GATC 3 cut(s) 90, 290, 822
Sau96I GGNCC 2 cut(s) 233, 840
SchI GAGTC 2 cut(s) 405, 709
ScrFI CCNGG 7 cut(s) 183, 383, 559, 573, 698, 844, 896
SduI GDGCHC 2 cut(s) 478, 673
SfaNI GCATC 4 cut(s) 218, 511, 598, 763
SfcI CTRYAG 1 cut(s) 246
SinI GGWCC 2 cut(s) 233, 840
SmiMI CAYNNNNRTG 1 cut(s) 942
SmlI CTYRAG 1 cut(s) 672
SmoI CTYRAG 1 cut(s) 672
Sse9I AATT 1 cut(s) 511
SsiI CCGC 6 cut(s) 72, 288, 471, 804, 827, 837
SspMI CTAG 3 cut(s) 188, 260, 284
SstI GAGCTC 1 cut(s) 673
StyD4I CCNGG 7 cut(s) 181, 381, 557, 571, 696, 842, 894
TaaI ACNGT 7 cut(s) 136, 223, 428, 490, 539, 613, 730
TaiI ACGT 1 cut(s) 282
TasI AATT 1 cut(s) 511
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 5 cut(s) 139, 408, 433, 919, 949
TseI GCWGC 1 cut(s) 578
TspGWI ACGGA 3 cut(s) 360, 522, 717
TspRI CASTG 5 cut(s) 139, 408, 433, 919, 949
VpaK11BI GGWCC 2 cut(s) 233, 840
XapI RAATTY 1 cut(s) 511
XmiI GTMKAC 1 cut(s) 948
XspI CTAG 3 cut(s) 188, 260, 284
Zsp2I ATGCAT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.