MD07G1189200.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0016187
Physical Location & Seq
Forward (+)
26959047 .. 26960234
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1189200.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGGCAAAGTCCCACAAATCGCTCAAGTCCACACTCTACATCTTGCTCCTAGCCATCACCCTTGGATTTGCCGGCTCAGCTAGGATCCTCGACGAGGCGCAACCCGTAGCATCCAATCCTCTTCAGACAACTGTCCCTACCACGAACCCTCTGCCAAGTGGCCAAATCCCTATCATCGCCCCCGCCACCACAACTGTTGATGACGATGTCGACGACGCTGACTCACCAATCCCTGAGACTGATGCCCCGCCAGAAGCTGACGTGGTGCCTCCTGTAGTGCCTCCTGTAGTCCCTCCTGTCACAACCGTCCCACAAGCTAACTCACCACTACCAGAGACACACGACGAACCTGCCATAGTGCCAACACCGGTTGCTAATGTGGCACCCGTAGCAACACCTGCCGTTCCAGTCACCACACCCGTGGCAGGTCCTGTCACCACATCGCCAACCGGGCCCGCCGCAACTGCCGCGGTCGCCAGAACGGAAACCCCGCATTTGTCCTTTTTCATGCACGACATCCTAGGAGGATCCCACCCATCAGTCAGAGTGGTGACCGGCCTCATCGCCAACACAGTCCTCAACCCCGCATTCTCCAAACCCAACAACAACATCTTCCCCGTCAGCGGTGGAACCCCACTATCCAACAACAACATCAATGGCTTCCTCAACAACAACAACATCCCCAACATCGCCGGCCTCAGTGGCCTCACCAACTCCCAATCCAGCACGGTCATACAGAACAGCGGCAACAACAACATCGTCAACGACGGCAGCAACCAGCCCTTCGTCACTGCCGGCCAGCTCCCCAACGGAGCCACCCTCCAGAAGCTCATGTTCGGCTCGGTGACCGTGATCGACGACGACCTGACCGAAGGACACGAGCTGGGGTCTGCCGTGCTCGGCAAGGCACAGGGCTTTTACCTGGCTAGCTCGCTGGACGGGAACAGCCACACCATGGCTTTTACTATGTTGCTGCACGCAGGTGAACATGATGTGGAGGACACCATTAGCCTATTTGGCGTCCACCGTACGGCGTCTCCGGTGTCCCACATTGCTGTGATTGGTGGGACTGGGAAGTACGAGAATGCGAACGGGTACGCCGCCATAGAGAGCCTGCACCAGGTGGACCAGCACACGACTGATGGGGTGGATACCATTATGCAGATCAGCGTTTACCTTTCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

396

Amino Acids

40.72

Weight (kDa)

4.6

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 253 - 393 1.4e-28 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39430 AT3G55230
fragaria_vesca FvH4_7g21000
malus_domestica MD01G1119900.v1.1 MD07G1189200.v1.1
prunus_persica Prupe.2G226700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365391
rosa_laevigata RLG00000027426
rosa_multiflora Rmu_sc0008527.1_g000004
rosa_roxburghii Rroxscaffold_4G00291260
rosa_rugosa Rorug01G0321600
rosa_samantha Rh1AG330500 Rh1BG292000 Rh1DG323600
rosa_wichuraiana Rw1G029300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 406, 971
Acc36I ACCTGC 4 cut(s) 358, 406, 416, 971
AccB1I GGYRCC 2 cut(s) 265, 382
AccB7I CCANNNNNTGG 1 cut(s) 955
AccI GTMKAC 1 cut(s) 210
AccII CGCG 1 cut(s) 470
AclWI GGATC 4 cut(s) 79, 92, 522, 535
AcoI YGGCCR 2 cut(s) 160, 796
AcuI CTGAAG 1 cut(s) 107
AcyI GRCGYC 2 cut(s) 1020, 1034
AdeI CACNNNGTG 1 cut(s) 1123
AfaI GTAC 3 cut(s) 1030, 1079, 1097
AfiI CCNNNNNNNGG 6 cut(s) 94, 425, 623, 955, 1030, 1120
AgeI ACCGGT 1 cut(s) 367
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 2 cut(s) 921, 1119
AleI CACNNNNGTG 2 cut(s) 419, 981
AluBI AGCT 7 cut(s) 80, 257, 317, 802, 829, 883, 930
AluI AGCT 7 cut(s) 80, 257, 317, 802, 829, 883, 930
Alw21I GWGCWC 1 cut(s) 900
Alw26I GTCTC 3 cut(s) 230, 329, 1041
AlwI GGATC 4 cut(s) 79, 92, 522, 535
AlwNI CAGNNNCTG 2 cut(s) 257, 431
AoxI GGCC 6 cut(s) 160, 452, 556, 694, 703, 796
ApaI GGGCCC 1 cut(s) 456
ApeKI GCWGC 2 cut(s) 771, 973
AsiGI ACCGGT 1 cut(s) 367
AspA2I CCTAGG 1 cut(s) 520
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 4 cut(s) 428, 452, 453, 1126
AsuC2I CCSGG 1 cut(s) 451
AsuHPI GGTGA 9 cut(s) 49, 216, 315, 403, 427, 562, 700, 856, 995
AsuNHI GCTAGC 1 cut(s) 926
AvaII GGWCC 2 cut(s) 428, 1126
AvrII CCTAGG 1 cut(s) 520
BaeGI GKGCMC 1 cut(s) 456
BalI TGGCCA 1 cut(s) 162
BamHI GGATCC 2 cut(s) 84, 527
BanI GGYRCC 2 cut(s) 265, 382
BanII GRGCYC 1 cut(s) 456
BauI CACGAG 1 cut(s) 878
Bbv12I GWGCWC 1 cut(s) 900
BbvI GCAGC 2 cut(s) 783, 960
BccI CCATC 3 cut(s) 62, 544, 1136
BceAI ACGGC 4 cut(s) 386, 784, 878, 1047
BcgI CGANNNNNNTGC 4 cut(s) 133, 167, 332, 366
BciT130I CCWGG 2 cut(s) 923, 1121
BciVI GTATCC 1 cut(s) 1144
BcnI CCSGG 1 cut(s) 451
BcoDI GTCTC 3 cut(s) 230, 329, 1041
BfaI CTAG 4 cut(s) 50, 81, 521, 927
BfmI CTRYAG 2 cut(s) 273, 285
BfuAI ACCTGC 4 cut(s) 358, 406, 416, 971
BfuI GTATCC 1 cut(s) 1144
BglI GCCNNNNNGGC 3 cut(s) 451, 702, 1017
BisI GCNGC 6 cut(s) 459, 468, 745, 772, 974, 1101
BlnI CCTAGG 1 cut(s) 520
BlpI GCTNAGC 1 cut(s) 76
BlsI GCNGC 6 cut(s) 460, 469, 746, 773, 975, 1102
Bme1390I CCNGG 3 cut(s) 451, 923, 1121
Bme18I GGWCC 2 cut(s) 428, 1126
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 4 cut(s) 428, 452, 453, 1126
BmiI GGNNCC 7 cut(s) 86, 267, 384, 454, 529, 631, 814
BmrFI CCNGG 3 cut(s) 451, 923, 1121
BmrI ACTGGG 1 cut(s) 1080
BmsI GCATC 2 cut(s) 119, 232
BmtI GCTAGC 1 cut(s) 930
BmuI ACTGGG 1 cut(s) 1080
BoxI GACNNNNGTC 1 cut(s) 131
BplI GAGNNNNNCTC 2 cut(s) 804, 836
BpmI CTGGAG 1 cut(s) 806
Bpu1102I GCTNAGC 1 cut(s) 76
BpuEI CTTGAG 1 cut(s) 8
BpuMI CCSGG 1 cut(s) 451
BsaHI GRCGYC 2 cut(s) 1020, 1034
BsaJI CCNNGG 5 cut(s) 61, 420, 468, 520, 954
BsaWI WCCGGW 2 cut(s) 367, 1039
BsaXI ACNNNNNCTCC 2 cut(s) 1021, 1051
Bsc4I CCNNNNNNNGG 6 cut(s) 94, 425, 623, 955, 1030, 1120
Bse118I RCCGGY 5 cut(s) 71, 367, 554, 692, 794
Bse1I ACTGG 2 cut(s) 407, 1075
Bse3DI GCAATG 1 cut(s) 1050
BseBI CCWGG 2 cut(s) 923, 1121
BseDI CCNNGG 5 cut(s) 61, 420, 468, 520, 954
BseGI GGATG 3 cut(s) 110, 516, 678
BseLI CCNNNNNNNGG 6 cut(s) 94, 425, 623, 955, 1030, 1120
BseMI GCAATG 1 cut(s) 1050
BseMII CTCAG 3 cut(s) 90, 225, 712
BseNI ACTGG 2 cut(s) 407, 1075
BseSI GKGCMC 1 cut(s) 456
BseXI GCAGC 2 cut(s) 783, 960
BseYI CCCAGC 1 cut(s) 883
BsgI GTGCAG 2 cut(s) 959, 1100
Bsh1236I CGCG 1 cut(s) 470
Bsh1285I CGRYCG 1 cut(s) 474
BshFI GGCC 6 cut(s) 162, 454, 558, 696, 705, 798
BshNI GGYRCC 2 cut(s) 265, 382
BshTI ACCGGT 1 cut(s) 367
BsiEI CGRYCG 1 cut(s) 474
BsiHKAI GWGCWC 1 cut(s) 900
BsiSI CCGG 7 cut(s) 72, 368, 450, 555, 693, 795, 1040
BsiWI CGTACG 1 cut(s) 1028
BslFI GGGAC 5 cut(s) 119, 275, 293, 1030, 1081
BslI CCNNNNNNNGG 6 cut(s) 94, 425, 623, 955, 1030, 1120
BsmAI GTCTC 3 cut(s) 230, 329, 1041
BsmBI CGTCTC 1 cut(s) 1041
BsmFI GGGAC 5 cut(s) 119, 275, 293, 1030, 1081
BsmI GAATGC 2 cut(s) 587, 1090
BsnI GGCC 6 cut(s) 162, 454, 558, 696, 705, 798
Bsp120I GGGCCC 1 cut(s) 452
Bsp1286I GDGCHC 2 cut(s) 456, 900
Bsp143I GATC 4 cut(s) 84, 527, 852, 1164
Bsp1720I GCTNAGC 1 cut(s) 76
Bsp19I CCATGG 1 cut(s) 954
BspANI GGCC 6 cut(s) 162, 454, 558, 696, 705, 798
BspCNI CTCAG 3 cut(s) 89, 226, 711
BspFNI CGCG 1 cut(s) 470
BspLI GGNNCC 7 cut(s) 86, 267, 384, 454, 529, 631, 814
BspMI ACCTGC 4 cut(s) 358, 406, 416, 971
BspOI GCTAGC 1 cut(s) 930
BspPI GGATC 4 cut(s) 79, 92, 522, 535
BspT107I GGYRCC 2 cut(s) 265, 382
BsrDI GCAATG 1 cut(s) 1050
BsrFI RCCGGY 5 cut(s) 71, 367, 554, 692, 794
BsrI ACTGG 2 cut(s) 407, 1075
BssAI RCCGGY 5 cut(s) 71, 367, 554, 692, 794
BssECI CCNNGG 5 cut(s) 61, 420, 468, 520, 954
BssMI GATC 4 cut(s) 84, 527, 852, 1164
BssNI GRCGYC 2 cut(s) 1020, 1034
BssSI CACGAG 1 cut(s) 878
BssT1I CCWWGG 3 cut(s) 61, 520, 954
Bst2BI CACGAG 1 cut(s) 878
Bst2UI CCWGG 2 cut(s) 923, 1121
Bst4CI ACNGT 7 cut(s) 133, 196, 307, 574, 730, 850, 1028
Bst6I CTCTTC 1 cut(s) 126
BstACI GRCGYC 2 cut(s) 1020, 1034
BstAPI GCANNNNNTGC 1 cut(s) 398
BstC8I GCNNGC 9 cut(s) 73, 456, 694, 796, 800, 928, 932, 978, 1115
BstDEI CTNAG 3 cut(s) 76, 234, 698
BstDSI CCRYGG 3 cut(s) 420, 468, 954
BstEII GGTNACC 2 cut(s) 550, 844
BstENI CCTNNNNNAGG 2 cut(s) 92, 1118
BstF5I GGATG 3 cut(s) 110, 516, 678
BstFNI CGCG 1 cut(s) 470
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 4 cut(s) 87, 530, 855, 1167
BstMAI GTCTC 3 cut(s) 230, 329, 1041
BstMBI GATC 4 cut(s) 84, 527, 852, 1164
BstMCI CGRYCG 1 cut(s) 474
BstNI CCWGG 2 cut(s) 923, 1121
BstPAI GACNNNNGTC 1 cut(s) 131
BstPI GGTNACC 2 cut(s) 550, 844
BstSCI CCNGG 3 cut(s) 449, 921, 1119
BstSFI CTRYAG 2 cut(s) 273, 285
BstSLI GKGCMC 1 cut(s) 456
BstUI CGCG 1 cut(s) 470
BstV1I GCAGC 2 cut(s) 783, 960
BstX2I RGATCY 2 cut(s) 84, 527
BstXI CCANNNNNNTGG 1 cut(s) 421
BstYI RGATCY 2 cut(s) 84, 527
BsuI GTATCC 1 cut(s) 1144
BsuRI GGCC 6 cut(s) 162, 454, 558, 696, 705, 798
BtgI CCRYGG 3 cut(s) 420, 468, 954
BtgZI GCGATG 4 cut(s) 160, 426, 547, 673
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 3 cut(s) 110, 516, 678
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 2 cut(s) 706, 789
BveI ACCTGC 4 cut(s) 358, 406, 416, 971
Cac8I GCNNGC 9 cut(s) 73, 456, 694, 796, 800, 928, 932, 978, 1115
CaiI CAGNNNCTG 2 cut(s) 257, 431
CfoI GCGC 1 cut(s) 100
Cfr10I RCCGGY 5 cut(s) 71, 367, 554, 692, 794
Cfr13I GGNCC 4 cut(s) 428, 452, 453, 1126
Cfr42I CCGCGG 1 cut(s) 471
CseI GACGC 3 cut(s) 224, 1009, 1023
CsiI ACCWGGT 1 cut(s) 1119
Csp6I GTAC 3 cut(s) 1029, 1078, 1096
CspAI ACCGGT 1 cut(s) 367
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviAII CATG 4 cut(s) 508, 832, 955, 989
CviQI GTAC 3 cut(s) 1029, 1078, 1096
DdeI CTNAG 3 cut(s) 76, 234, 698
DpnI GATC 4 cut(s) 86, 529, 854, 1166
DpnII GATC 4 cut(s) 84, 527, 852, 1164
DraIII CACNNNGTG 1 cut(s) 1123
EaeI YGGCCR 2 cut(s) 160, 796
Eam1104I CTCTTC 1 cut(s) 126
EarI CTCTTC 1 cut(s) 126
Eco130I CCWWGG 3 cut(s) 61, 520, 954
Eco24I GRGCYC 1 cut(s) 456
Eco47I GGWCC 2 cut(s) 428, 1126
Eco57I CTGAAG 1 cut(s) 107
Eco91I GGTNACC 2 cut(s) 550, 844
EcoNI CCTNNNNNAGG 2 cut(s) 92, 1118
EcoO109I RGGNCCY 1 cut(s) 428
EcoO65I GGTNACC 2 cut(s) 550, 844
EcoRII CCWGG 2 cut(s) 921, 1119
EcoT14I CCWWGG 3 cut(s) 61, 520, 954
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 3 cut(s) 61, 520, 954
Esp3I CGTCTC 1 cut(s) 1041
FaeI CATG 4 cut(s) 511, 835, 958, 992
FaiI YATR 9 cut(s) 356, 509, 734, 833, 956, 968, 990, 1106, 1160
FaqI GGGAC 5 cut(s) 119, 275, 293, 1030, 1081
FatI CATG 4 cut(s) 507, 831, 954, 988
FauI CCCGC 5 cut(s) 190, 255, 463, 498, 592
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 6 cut(s) 459, 468, 745, 772, 974, 1101
FokI GGATG 3 cut(s) 97, 503, 665
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 6 cut(s) 459, 468, 745, 772, 974, 1101
FspBI CTAG 4 cut(s) 50, 81, 521, 927
GlaI GCGC 1 cut(s) 99
GluI GCNGC 6 cut(s) 459, 468, 745, 772, 974, 1101
GsaI CCCAGC 1 cut(s) 887
GsuI CTGGAG 1 cut(s) 806
HaeIII GGCC 6 cut(s) 162, 454, 558, 696, 705, 798
HapII CCGG 7 cut(s) 72, 368, 450, 555, 693, 795, 1040
HgaI GACGC 3 cut(s) 224, 1009, 1023
HhaI GCGC 1 cut(s) 100
Hin1I GRCGYC 2 cut(s) 1020, 1034
Hin1II CATG 4 cut(s) 511, 835, 958, 992
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HincII GTYRAC 2 cut(s) 211, 763
HindII GTYRAC 2 cut(s) 211, 763
HinfI GANTC 1 cut(s) 221
HpaII CCGG 7 cut(s) 72, 368, 450, 555, 693, 795, 1040
HphI GGTGA 9 cut(s) 49, 216, 315, 403, 427, 562, 700, 856, 995
Hpy166II GTNNAC 7 cut(s) 30, 211, 763, 986, 1024, 1126, 1174
Hpy188I TCNGA 3 cut(s) 126, 545, 1183
Hpy188III TCNNGA 1 cut(s) 823
Hpy8I GTNNAC 7 cut(s) 30, 211, 763, 986, 1024, 1126, 1174
Hpy99I CGWCG 7 cut(s) 95, 215, 218, 347, 770, 860, 863
HpyAV CCTTC 2 cut(s) 793, 866
HpyCH4III ACNGT 7 cut(s) 133, 196, 307, 574, 730, 850, 1028
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 4 cut(s) 511, 976, 1117, 1162
HpyF3I CTNAG 3 cut(s) 76, 234, 698
HpySE526I ACGT 1 cut(s) 261
Hsp92I GRCGYC 2 cut(s) 1020, 1034
Hsp92II CATG 4 cut(s) 511, 835, 958, 992
HspAI GCGC 1 cut(s) 98
KroI GCCGGC 3 cut(s) 71, 692, 794
KroNI GCCGGC 3 cut(s) 73, 694, 796
KspI CCGCGG 1 cut(s) 471
Kzo9I GATC 4 cut(s) 84, 527, 852, 1164
LmnI GCTCC 3 cut(s) 51, 807, 812
Lsp1109I GCAGC 2 cut(s) 783, 960
LweI GCATC 2 cut(s) 119, 232
MabI ACCWGGT 1 cut(s) 1119
MaeI CTAG 4 cut(s) 50, 81, 521, 927
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 6 cut(s) 298, 409, 433, 550, 787, 844
MalI GATC 4 cut(s) 86, 529, 854, 1166
MboI GATC 4 cut(s) 84, 527, 852, 1164
MboII GAAGA 2 cut(s) 113, 604
MflI RGATCY 2 cut(s) 84, 527
MhlI GDGCHC 2 cut(s) 456, 900
MlsI TGGCCA 1 cut(s) 162
MluNI TGGCCA 1 cut(s) 162
MlyI GAGTC 1 cut(s) 215
MmeI TCCRAC 1 cut(s) 666
Mox20I TGGCCA 1 cut(s) 162
MroNI GCCGGC 3 cut(s) 71, 692, 794
MscI TGGCCA 1 cut(s) 162
MslI CAYNNNNRTG 3 cut(s) 419, 981, 1055
Msp20I TGGCCA 1 cut(s) 162
MspA1I CMGCKG 3 cut(s) 470, 624, 744
MspI CCGG 7 cut(s) 72, 368, 450, 555, 693, 795, 1040
MspR9I CCNGG 3 cut(s) 451, 923, 1121
Mva1269I GAATGC 2 cut(s) 587, 1090
MvaI CCWGG 2 cut(s) 923, 1121
MvnI CGCG 1 cut(s) 470
NaeI GCCGGC 3 cut(s) 73, 694, 796
NciI CCSGG 1 cut(s) 451
NcoI CCATGG 1 cut(s) 954
NdeII GATC 4 cut(s) 84, 527, 852, 1164
NgoMIV GCCGGC 3 cut(s) 71, 692, 794
NheI GCTAGC 1 cut(s) 926
NlaIII CATG 4 cut(s) 511, 835, 958, 992
NlaIV GGNNCC 7 cut(s) 86, 267, 384, 454, 529, 631, 814
NmeAIII GCCGAG 1 cut(s) 879
NmuCI GTSAC 6 cut(s) 298, 409, 433, 550, 787, 844
OliI CACNNNNGTG 2 cut(s) 419, 981
PaqCI CACCTGC 2 cut(s) 406, 971
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 867
PctI GAATGC 2 cut(s) 587, 1090
PdiI GCCGGC 3 cut(s) 73, 694, 796
Pfl23II CGTACG 1 cut(s) 1028
PflFI GACNNNGTC 1 cut(s) 206
PflMI CCANNNNNTGG 1 cut(s) 955
PinAI ACCGGT 1 cut(s) 367
PkrI GCNGC 6 cut(s) 460, 469, 746, 773, 975, 1102
PleI GAGTC 1 cut(s) 215
PpsI GAGTC 1 cut(s) 215
PpuMI RGGWCCY 1 cut(s) 428
PshAI GACNNNNGTC 1 cut(s) 131
Psp5II RGGWCCY 1 cut(s) 428
Psp6I CCWGG 2 cut(s) 921, 1119
PspEI GGTNACC 2 cut(s) 550, 844
PspFI CCCAGC 1 cut(s) 883
PspGI CCWGG 2 cut(s) 921, 1119
PspLI CGTACG 1 cut(s) 1028
PspN4I GGNNCC 7 cut(s) 86, 267, 384, 454, 529, 631, 814
PspOMI GGGCCC 1 cut(s) 452
PspPI GGNCC 4 cut(s) 428, 452, 453, 1126
PspPPI RGGWCCY 1 cut(s) 428
PstNI CAGNNNCTG 2 cut(s) 257, 431
PsuI RGATCY 2 cut(s) 84, 527
PsyI GACNNNGTC 1 cut(s) 206
RsaI GTAC 3 cut(s) 1030, 1079, 1097
RsaNI GTAC 3 cut(s) 1029, 1078, 1096
RseI CAYNNNNRTG 3 cut(s) 419, 981, 1055
SacII CCGCGG 1 cut(s) 471
SalI GTCGAC 1 cut(s) 209
SatI GCNGC 6 cut(s) 459, 468, 745, 772, 974, 1101
Sau3AI GATC 4 cut(s) 84, 527, 852, 1164
Sau96I GGNCC 4 cut(s) 428, 452, 453, 1126
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 3 cut(s) 451, 923, 1121
SduI GDGCHC 2 cut(s) 456, 900
SexAI ACCWGGT 1 cut(s) 1119
SfaNI GCATC 2 cut(s) 119, 232
SfcI CTRYAG 2 cut(s) 273, 285
SfiI GGCCNNNNNGGCC 1 cut(s) 702
Sfr303I CCGCGG 1 cut(s) 471
SgrBI CCGCGG 1 cut(s) 471
SinI GGWCC 2 cut(s) 428, 1126
SmiMI CAYNNNNRTG 3 cut(s) 419, 981, 1055
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
SspMI CTAG 4 cut(s) 50, 81, 521, 927
StyD4I CCNGG 3 cut(s) 449, 921, 1119
StyI CCWWGG 3 cut(s) 61, 520, 954
TaaI ACNGT 7 cut(s) 133, 196, 307, 574, 730, 850, 1028
TaiI ACGT 1 cut(s) 264
TaqI TCGA 3 cut(s) 90, 210, 855
TaqII GACCGA 1 cut(s) 884
TauI GCSGC 4 cut(s) 461, 470, 747, 1103
TscAI CASTG 2 cut(s) 706, 796
TseFI GTSAC 6 cut(s) 298, 409, 433, 550, 787, 844
TseI GCWGC 2 cut(s) 771, 973
Tsp45I GTSAC 6 cut(s) 298, 409, 433, 550, 787, 844
TspDTI ATGAA 1 cut(s) 496
TspGWI ACGGA 2 cut(s) 497, 825
TspRI CASTG 2 cut(s) 706, 796
Tth111I GACNNNGTC 1 cut(s) 206
Van91I CCANNNNNTGG 1 cut(s) 955
VpaK11BI GGWCC 2 cut(s) 428, 1126
XagI CCTNNNNNAGG 2 cut(s) 92, 1118
XmaJI CCTAGG 1 cut(s) 520
XmiI GTMKAC 1 cut(s) 210
XspI CTAG 4 cut(s) 50, 81, 521, 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.