Rorug01G0321600

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0016187
Physical Location & Seq
Forward (+)
44061442 .. 44063272
1831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0321600.1

Sequence Viewer

Length: 897 bp
ATGGCAGCCACTTGCTTAATCAGAGCACCCTCTCCTCCCTCTTGGATCACTAACAACACCACCCCGAAGACCGGAGTCACAATTTCACTTAAACGATTCGTTTTCAGTTCCGTTCCATTTTCTTCCTTGCATCACCAGATCAGGTACTCGAAGAACAGAGCCGGGGTTTTGACGGCGTTGGCCGTAGTGGAACAGGAACAGGCGGTGAGTTCAGTGGCTGAGGAAGAAGAACAAGGTGTGGACGATAATGGGTATGAAGAGCAGTATGAGCACGAGAGACACAGCGCCGATGTTTCAGGCGAGGAGGGATTGGAAATGGAGAAGCAGAATCGGCAGCCAAGACCGTGTGAGTTGTACGTGTGCAATCTTCCCAGAAGCTCTGACATTGAGGACTTGATGGAGATGTTTAAGCCACACGGGACTGTTTTCTCCGTCGAGATTTCCCGGAGTCCGGTGACCGGTTTAAGCAGAGGCTGTGGTTACATTACAATGGGCTCTATAGACTCCGCTAAAGCCGCCATTGCTGCATTGGATGGGATTGATGTTGCAGGGAGGGAGATGCGGGTTAGGTTTTCGGTTCATATGAGTCCGAGGACTAGGAACTCCGAGGCATTGAATTCGGCACCGGTGAAGAACTTGGTGTACGAAAGCCCTTATAAGCTCTATGTTGGTAACCTTGCTTGGGCTGTCCAGCCTCATGCTTTGAGGAGCCATTTCAGCAAGTTTGGGACTGTGGCTAGTGCAAGAGTGTTGCATGATCGTAAAGCTGGGAAGAACCGGGCTTATGGGTTTATCTCATTCTCTTCGGCTGCGGAGCGTGATGAAGCTGCCACTTTGGATGGAACGGAGTTTTTTGGTCGGAGGATAATAGTTAGAGGACTTAAAGAAAGGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

298

Amino Acids

32.93

Weight (kDa)

7.13

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 118 - 188 3.6e-13 RNA recognition motif
RRM_1 PF00076 221 - 289 2e-14 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39430 AT3G55230
fragaria_vesca FvH4_7g21000
malus_domestica MD01G1119900.v1.1 MD07G1189200.v1.1
prunus_persica Prupe.2G226700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365391
rosa_laevigata RLG00000027426
rosa_multiflora Rmu_sc0008527.1_g000004
rosa_roxburghii Rroxscaffold_4G00291260
rosa_rugosa Rorug01G0321600
rosa_samantha Rh1AG330500 Rh1BG292000 Rh1DG323600
rosa_wichuraiana Rw1G029300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 657
AccB1I GGYRCC 1 cut(s) 622
AciI CCGC 5 cut(s) 203, 507, 516, 562, 812
AclWI GGATC 1 cut(s) 53
AcoI YGGCCR 1 cut(s) 180
AcsI RAATTY 1 cut(s) 616
AfaI GTAC 3 cut(s) 146, 356, 644
AfiI CCNNNNNNNGG 4 cut(s) 71, 451, 458, 682
AflIII ACRYGT 1 cut(s) 357
AgeI ACCGGT 2 cut(s) 458, 625
AgsI TTSAA 1 cut(s) 616
AluBI AGCT 4 cut(s) 378, 661, 767, 827
AluI AGCT 4 cut(s) 378, 661, 767, 827
Alw21I GWGCWC 2 cut(s) 28, 273
Alw26I GTCTC 1 cut(s) 271
AlwI GGATC 1 cut(s) 53
AlwNI CAGNNNCTG 2 cut(s) 218, 474
AoxI GGCC 1 cut(s) 180
ApeKI GCWGC 5 cut(s) 5, 334, 524, 809, 827
ApoI RAATTY 1 cut(s) 616
AsiGI ACCGGT 2 cut(s) 458, 625
AspLEI GCGC 1 cut(s) 287
AsuC2I CCSGG 3 cut(s) 163, 445, 779
AsuHPI GGTGA 4 cut(s) 125, 217, 466, 640
BanI GGYRCC 1 cut(s) 622
BanII GRGCYC 1 cut(s) 497
BauI CACGAG 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 74
Bbv12I GWGCWC 2 cut(s) 28, 273
BbvCI CCTCAGC 1 cut(s) 219
BbvI GCAGC 5 cut(s) 17, 346, 511, 796, 814
BccI CCATC 3 cut(s) 391, 527, 833
BceAI ACGGC 2 cut(s) 167, 189
BcgI CGANNNNNNTGC 2 cut(s) 600, 634
BcnI CCSGG 3 cut(s) 163, 445, 779
BcoDI GTCTC 1 cut(s) 271
BfaI CTAG 2 cut(s) 597, 738
BfmI CTRYAG 1 cut(s) 498
BfoI RGCGCY 1 cut(s) 288
BisI GCNGC 6 cut(s) 6, 335, 516, 525, 810, 828
BlsI GCNGC 6 cut(s) 7, 336, 517, 526, 811, 829
Bme1390I CCNGG 3 cut(s) 163, 445, 779
BmiI GGNNCC 2 cut(s) 624, 710
BmrFI CCNGG 3 cut(s) 163, 445, 779
BmsI GCATC 2 cut(s) 139, 549
BoxI GACNNNNGTC 1 cut(s) 74
BpiI GAAGAC 1 cut(s) 74
Bpu10I CCTNAGC 1 cut(s) 219
BpuMI CCSGG 3 cut(s) 163, 445, 779
BsaAI YACGTR 1 cut(s) 358
BsaJI CCNNGG 3 cut(s) 162, 590, 606
BsaWI WCCGGW 4 cut(s) 71, 451, 458, 625
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 451, 458, 682
Bse118I RCCGGY 2 cut(s) 458, 625
Bse3DI GCAATG 1 cut(s) 519
BseDI CCNNGG 3 cut(s) 162, 590, 606
BseGI GGATG 2 cut(s) 538, 844
BseLI CCNNNNNNNGG 4 cut(s) 71, 451, 458, 682
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 1 cut(s) 210
BseRI GAGGAG 3 cut(s) 24, 317, 721
BseXI GCAGC 5 cut(s) 17, 346, 511, 796, 814
BseYI CCCAGC 1 cut(s) 767
BshFI GGCC 1 cut(s) 182
BshNI GGYRCC 1 cut(s) 622
BshTI ACCGGT 2 cut(s) 458, 625
BsiHKAI GWGCWC 2 cut(s) 28, 273
BsiSI CCGG 7 cut(s) 72, 162, 445, 452, 459, 626, 778
BslFI GGGAC 2 cut(s) 433, 742
BslI CCNNNNNNNGG 4 cut(s) 71, 451, 458, 682
BsmAI GTCTC 1 cut(s) 271
BsmFI GGGAC 2 cut(s) 433, 742
BsnI GGCC 1 cut(s) 182
Bsp1286I GDGCHC 3 cut(s) 28, 273, 497
Bsp143I GATC 3 cut(s) 45, 138, 757
BspACI CCGC 5 cut(s) 203, 507, 516, 562, 812
BspANI GGCC 1 cut(s) 182
BspCNI CTCAG 1 cut(s) 211
BspLI GGNNCC 2 cut(s) 624, 710
BspPI GGATC 1 cut(s) 53
BspQI GCTCTTC 1 cut(s) 252
BspT107I GGYRCC 1 cut(s) 622
BsrDI GCAATG 1 cut(s) 519
BsrFI RCCGGY 2 cut(s) 458, 625
BssAI RCCGGY 2 cut(s) 458, 625
BssECI CCNNGG 3 cut(s) 162, 590, 606
BssMI GATC 3 cut(s) 45, 138, 757
BssSI CACGAG 1 cut(s) 272
Bst2BI CACGAG 1 cut(s) 272
Bst4CI ACNGT 3 cut(s) 345, 424, 733
Bst6I CTCTTC 2 cut(s) 252, 808
BstBAI YACGTR 1 cut(s) 358
BstDEI CTNAG 2 cut(s) 219, 894
BstEII GGTNACC 2 cut(s) 454, 671
BstF5I GGATG 2 cut(s) 538, 844
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 3 cut(s) 48, 141, 760
BstMAI GTCTC 1 cut(s) 271
BstMBI GATC 3 cut(s) 45, 138, 757
BstMWI GCNNNNNNNGC 6 cut(s) 268, 331, 515, 521, 524, 717
BstPAI GACNNNNGTC 1 cut(s) 74
BstPI GGTNACC 2 cut(s) 454, 671
BstSCI CCNGG 3 cut(s) 161, 443, 777
BstSFI CTRYAG 1 cut(s) 498
BstV1I GCAGC 5 cut(s) 17, 346, 511, 796, 814
BstV2I GAAGAC 1 cut(s) 74
BsuRI GGCC 1 cut(s) 182
BtsCI GGATG 2 cut(s) 538, 844
BtsIMutI CAGTG 1 cut(s) 219
CaiI CAGNNNCTG 2 cut(s) 218, 474
CfoI GCGC 1 cut(s) 287
Cfr10I RCCGGY 2 cut(s) 458, 625
Csp6I GTAC 3 cut(s) 145, 355, 643
CspAI ACCGGT 2 cut(s) 458, 625
CviAII CATG 2 cut(s) 698, 755
CviQI GTAC 3 cut(s) 145, 355, 643
DdeI CTNAG 2 cut(s) 219, 894
DpnI GATC 3 cut(s) 47, 140, 759
DpnII GATC 3 cut(s) 45, 138, 757
EaeI YGGCCR 1 cut(s) 180
Eam1104I CTCTTC 2 cut(s) 252, 808
EarI CTCTTC 2 cut(s) 252, 808
Eco24I GRGCYC 1 cut(s) 497
Eco91I GGTNACC 2 cut(s) 454, 671
EcoO65I GGTNACC 2 cut(s) 454, 671
EcoRI GAATTC 1 cut(s) 616
EcoT38I GRGCYC 1 cut(s) 497
FaeI CATG 2 cut(s) 701, 758
FaqI GGGAC 2 cut(s) 433, 742
FatI CATG 2 cut(s) 697, 754
FauI CCCGC 1 cut(s) 555
FauNDI CATATG 1 cut(s) 582
Fnu4HI GCNGC 6 cut(s) 6, 335, 516, 525, 810, 828
FokI GGATG 2 cut(s) 545, 851
FriOI GRGCYC 1 cut(s) 497
Fsp4HI GCNGC 6 cut(s) 6, 335, 516, 525, 810, 828
FspBI CTAG 2 cut(s) 597, 738
GlaI GCGC 1 cut(s) 286
GluI GCNGC 6 cut(s) 6, 335, 516, 525, 810, 828
GsaI CCCAGC 1 cut(s) 771
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 1 cut(s) 182
HapII CCGG 7 cut(s) 72, 162, 445, 452, 459, 626, 778
HhaI GCGC 1 cut(s) 287
Hin1II CATG 2 cut(s) 701, 758
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HinfI GANTC 6 cut(s) 75, 96, 328, 448, 503, 586
HpaII CCGG 7 cut(s) 72, 162, 445, 452, 459, 626, 778
HphI GGTGA 4 cut(s) 125, 217, 466, 640
Hpy166II GTNNAC 2 cut(s) 241, 643
Hpy188I TCNGA 5 cut(s) 23, 382, 591, 607, 861
Hpy188III TCNNGA 1 cut(s) 436
Hpy8I GTNNAC 2 cut(s) 241, 643
Hpy99I CGWCG 1 cut(s) 437
HpyCH4III ACNGT 3 cut(s) 345, 424, 733
HpyCH4IV ACGT 1 cut(s) 357
HpyCH4V TGCA 6 cut(s) 130, 363, 527, 548, 743, 754
HpyF10VI GCNNNNNNNGC 6 cut(s) 268, 331, 515, 521, 524, 717
HpyF3I CTNAG 2 cut(s) 219, 894
HpySE526I ACGT 1 cut(s) 357
Hsp92II CATG 2 cut(s) 701, 758
HspAI GCGC 1 cut(s) 285
Kzo9I GATC 3 cut(s) 45, 138, 757
LguI GCTCTTC 1 cut(s) 252
LmnI GCTCC 2 cut(s) 708, 814
Lsp1109I GCAGC 5 cut(s) 17, 346, 511, 796, 814
LweI GCATC 2 cut(s) 139, 549
MaeI CTAG 2 cut(s) 597, 738
MaeII ACGT 1 cut(s) 357
MaeIII GTNAC 4 cut(s) 76, 454, 479, 671
MalI GATC 3 cut(s) 47, 140, 759
MboI GATC 3 cut(s) 45, 138, 757
MhlI GDGCHC 3 cut(s) 28, 273, 497
MluCI AATT 2 cut(s) 81, 616
MlyI GAGTC 4 cut(s) 84, 457, 497, 595
MmeI TCCRAC 1 cut(s) 839
MseI TTAA 5 cut(s) 17, 90, 408, 464, 882
MslI CAYNNNNRTG 1 cut(s) 488
MspI CCGG 7 cut(s) 72, 162, 445, 452, 459, 626, 778
MspR9I CCNGG 3 cut(s) 163, 445, 779
MwoI GCNNNNNNNGC 6 cut(s) 268, 331, 515, 521, 524, 717
NciI CCSGG 3 cut(s) 163, 445, 779
NdeI CATATG 1 cut(s) 582
NdeII GATC 3 cut(s) 45, 138, 757
NlaIII CATG 2 cut(s) 701, 758
NlaIV GGNNCC 2 cut(s) 624, 710
NmuCI GTSAC 2 cut(s) 76, 454
PciSI GCTCTTC 1 cut(s) 252
PfeI GAWTC 2 cut(s) 96, 328
PfoI TCCNGGA 1 cut(s) 443
PinAI ACCGGT 2 cut(s) 458, 625
PkrI GCNGC 6 cut(s) 7, 336, 517, 526, 811, 829
PleI GAGTC 4 cut(s) 83, 456, 497, 594
PpsI GAGTC 4 cut(s) 83, 456, 497, 594
Ppu21I YACGTR 1 cut(s) 358
PshAI GACNNNNGTC 1 cut(s) 74
PsiI TTATAA 1 cut(s) 657
PspEI GGTNACC 2 cut(s) 454, 671
PspFI CCCAGC 1 cut(s) 767
PspN4I GGNNCC 2 cut(s) 624, 710
PsrI GAACNNNNNNTAC 2 cut(s) 626, 658
PstNI CAGNNNCTG 2 cut(s) 218, 474
RsaI GTAC 3 cut(s) 146, 356, 644
RsaNI GTAC 3 cut(s) 145, 355, 643
RseI CAYNNNNRTG 1 cut(s) 488
SapI GCTCTTC 1 cut(s) 252
SaqAI TTAA 5 cut(s) 17, 90, 408, 464, 882
SatI GCNGC 6 cut(s) 6, 335, 516, 525, 810, 828
Sau3AI GATC 3 cut(s) 45, 138, 757
SchI GAGTC 4 cut(s) 84, 457, 497, 595
ScrFI CCNGG 3 cut(s) 163, 445, 779
SduI GDGCHC 3 cut(s) 28, 273, 497
SetI ASST 9 cut(s) 146, 238, 360, 380, 572, 663, 678, 769, 829
SfaNI GCATC 2 cut(s) 139, 549
SfcI CTRYAG 1 cut(s) 498
SgrAI CRCCGGYG 1 cut(s) 625
SmiMI CAYNNNNRTG 1 cut(s) 488
Sse9I AATT 2 cut(s) 81, 616
SsiI CCGC 5 cut(s) 203, 507, 516, 562, 812
SspMI CTAG 2 cut(s) 597, 738
StyD4I CCNGG 3 cut(s) 161, 443, 777
TaaI ACNGT 3 cut(s) 345, 424, 733
TaiI ACGT 1 cut(s) 360
TaqI TCGA 2 cut(s) 149, 435
TasI AATT 2 cut(s) 81, 616
TauI GCSGC 1 cut(s) 518
TfiI GAWTC 2 cut(s) 96, 328
Tru1I TTAA 5 cut(s) 17, 90, 408, 464, 882
Tru9I TTAA 5 cut(s) 17, 90, 408, 464, 882
TscAI CASTG 1 cut(s) 219
TseFI GTSAC 2 cut(s) 76, 454
TseI GCWGC 5 cut(s) 5, 334, 524, 809, 827
Tsp45I GTSAC 2 cut(s) 76, 454
TspDTI ATGAA 3 cut(s) 270, 569, 837
TspGWI ACGGA 3 cut(s) 100, 421, 860
TspRI CASTG 1 cut(s) 219
XapI RAATTY 1 cut(s) 616
XcmI CCANNNNNNNNNTGG 1 cut(s) 526
XspI CTAG 2 cut(s) 597, 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.