Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G39430 AT3G55230 |
| fragaria_vesca | FvH4_7g21000 |
| malus_domestica | MD01G1119900.v1.1 MD07G1189200.v1.1 |
| prunus_persica | Prupe.2G226700_v2.0.a1 |
| rosa_chinensis | RchiOBHm_Chr1g0365391 |
| rosa_laevigata | RLG00000027426 |
| rosa_multiflora | Rmu_sc0008527.1_g000004 |
| rosa_roxburghii | Rroxscaffold_4G00291260 |
| rosa_rugosa | Rorug01G0321600 |
| rosa_samantha | Rh1AG330500 Rh1BG292000 Rh1DG323600 |
| rosa_wichuraiana | Rw1G029300 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 455 |
| AccB1I | GGYRCC | 3 cut(s) | 280, 400, 539 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1021 |
| AclI | AACGTT | 1 cut(s) | 822 |
| AclWI | GGATC | 4 cut(s) | 91, 104, 595, 793 |
| AcoI | YGGCCR | 3 cut(s) | 3, 193, 1176 |
| AcyI | GRCGYC | 1 cut(s) | 540 |
| AdeI | CACNNNGTG | 1 cut(s) | 407 |
| AfaI | GTAC | 4 cut(s) | 312, 436, 1105, 1154 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 106, 392, 410, 464, 683, 1021, 1195 |
| AgsI | TTSAA | 1 cut(s) | 640 |
| AjiI | CACGTC | 1 cut(s) | 379 |
| AjnI | CCWGG | 2 cut(s) | 535, 618 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 620, 652 |
| AleI | CACNNNNGTG | 1 cut(s) | 458 |
| AluBI | AGCT | 8 cut(s) | 92, 320, 868, 895, 931, 949, 996, 1086 |
| AluI | AGCT | 8 cut(s) | 92, 320, 868, 895, 931, 949, 996, 1086 |
| Alw26I | GTCTC | 3 cut(s) | 15, 263, 332 |
| AlwI | GGATC | 4 cut(s) | 91, 104, 595, 793 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 272, 320 |
| Ama87I | CYCGRG | 1 cut(s) | 335 |
| ApeKI | GCWGC | 3 cut(s) | 789, 837, 868 |
| AspLEI | GCGC | 2 cut(s) | 542, 1058 |
| AspS9I | GGNCC | 9 cut(s) | 58, 382, 413, 467, 491, 690, 1096, 1143, 1201 |
| AsuC2I | CCSGG | 2 cut(s) | 405, 1147 |
| AsuHPI | GGTGA | 8 cut(s) | 295, 420, 474, 757, 766, 922, 928, 1021 |
| AvaI | CYCGRG | 1 cut(s) | 335 |
| AvaII | GGWCC | 7 cut(s) | 58, 413, 467, 690, 1096, 1143, 1201 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 294, 327 |
| BalI | TGGCCA | 2 cut(s) | 5, 195 |
| BamHI | GGATCC | 1 cut(s) | 96 |
| BanI | GGYRCC | 3 cut(s) | 280, 400, 539 |
| BauI | CACGAG | 1 cut(s) | 944 |
| BbvI | GCAGC | 3 cut(s) | 801, 849, 855 |
| BccI | CCATC | 5 cut(s) | 74, 215, 604, 1153, 1211 |
| BceAI | ACGGC | 3 cut(s) | 733, 873, 944 |
| BciT130I | CCWGG | 2 cut(s) | 537, 620 |
| BcnI | CCSGG | 2 cut(s) | 405, 1147 |
| BcoDI | GTCTC | 3 cut(s) | 15, 263, 332 |
| BfaI | CTAG | 3 cut(s) | 62, 93, 581 |
| BfoI | RGCGCY | 1 cut(s) | 543 |
| BfuAI | ACCTGC | 1 cut(s) | 455 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 768 |
| Bme1390I | CCNGG | 4 cut(s) | 405, 537, 620, 1147 |
| Bme18I | GGWCC | 7 cut(s) | 58, 413, 467, 690, 1096, 1143, 1201 |
| BmeT110I | CYCGRG | 1 cut(s) | 335 |
| BmgBI | CACGTC | 1 cut(s) | 379 |
| BmgT120I | GGNCC | 9 cut(s) | 58, 382, 413, 467, 491, 690, 1096, 1143, 1201 |
| BmrFI | CCNGG | 4 cut(s) | 405, 537, 620, 1147 |
| BmrI | ACTGGG | 4 cut(s) | 440, 498, 671, 1176 |
| BmsI | GCATC | 3 cut(s) | 131, 238, 762 |
| BmuI | ACTGGG | 4 cut(s) | 440, 498, 671, 1176 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 870, 902 |
| BpmI | CTGGAG | 2 cut(s) | 872, 897 |
| BpuEI | CTTGAG | 1 cut(s) | 20 |
| BpuMI | CCSGG | 2 cut(s) | 405, 1147 |
| BsaHI | GRCGYC | 1 cut(s) | 540 |
| BsaJI | CCNNGG | 5 cut(s) | 73, 459, 525, 654, 1020 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 106, 392, 410, 464, 683, 1021, 1195 |
| Bse118I | RCCGGY | 1 cut(s) | 767 |
| Bse1I | ACTGG | 7 cut(s) | 287, 446, 493, 619, 677, 880, 1171 |
| Bse3DI | GCAATG | 2 cut(s) | 182, 1125 |
| BseBI | CCWGG | 2 cut(s) | 537, 620 |
| BseDI | CCNNGG | 5 cut(s) | 73, 459, 525, 654, 1020 |
| BseGI | GGATG | 4 cut(s) | 69, 122, 250, 744 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 106, 392, 410, 464, 683, 1021, 1195 |
| BseMI | GCAATG | 2 cut(s) | 182, 1125 |
| BseMII | CTCAG | 3 cut(s) | 102, 176, 333 |
| BseNI | ACTGG | 7 cut(s) | 287, 446, 493, 619, 677, 880, 1171 |
| BseXI | GCAGC | 3 cut(s) | 801, 849, 855 |
| BseYI | CCCAGC | 2 cut(s) | 392, 949 |
| BshNI | GGYRCC | 3 cut(s) | 280, 400, 539 |
| BsiHKCI | CYCGRG | 1 cut(s) | 335 |
| BsiSI | CCGG | 3 cut(s) | 405, 768, 1146 |
| BslFI | GGGAC | 4 cut(s) | 131, 434, 703, 1156 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 106, 392, 410, 464, 683, 1021, 1195 |
| BsmAI | GTCTC | 3 cut(s) | 15, 263, 332 |
| BsmFI | GGGAC | 4 cut(s) | 131, 434, 703, 1156 |
| BsoBI | CYCGRG | 1 cut(s) | 335 |
| Bsp143I | GATC | 5 cut(s) | 96, 587, 798, 918, 1239 |
| Bsp19I | CCATGG | 1 cut(s) | 1020 |
| BspCNI | CTCAG | 3 cut(s) | 101, 175, 334 |
| BspMI | ACCTGC | 1 cut(s) | 455 |
| BspPI | GGATC | 4 cut(s) | 91, 104, 595, 793 |
| BspT107I | GGYRCC | 3 cut(s) | 280, 400, 539 |
| BsrDI | GCAATG | 2 cut(s) | 182, 1125 |
| BsrFI | RCCGGY | 1 cut(s) | 767 |
| BsrI | ACTGG | 7 cut(s) | 287, 446, 493, 619, 677, 880, 1171 |
| BssAI | RCCGGY | 1 cut(s) | 767 |
| BssECI | CCNNGG | 5 cut(s) | 73, 459, 525, 654, 1020 |
| BssMI | GATC | 5 cut(s) | 96, 587, 798, 918, 1239 |
| BssNI | GRCGYC | 1 cut(s) | 540 |
| BssSI | CACGAG | 1 cut(s) | 944 |
| BssT1I | CCWWGG | 3 cut(s) | 73, 654, 1020 |
| Bst2BI | CACGAG | 1 cut(s) | 944 |
| Bst2UI | CCWGG | 2 cut(s) | 537, 620 |
| Bst4CI | ACNGT | 9 cut(s) | 229, 310, 358, 526, 634, 796, 916, 1033, 1103 |
| BstACI | GRCGYC | 1 cut(s) | 540 |
| BstC8I | GCNNGC | 6 cut(s) | 363, 517, 760, 862, 866, 994 |
| BstDEI | CTNAG | 3 cut(s) | 88, 162, 342 |
| BstDSI | CCRYGG | 3 cut(s) | 459, 525, 1020 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 104, 1193 |
| BstF5I | GGATG | 4 cut(s) | 69, 122, 250, 744 |
| BstH2I | RGCGCY | 1 cut(s) | 543 |
| BstHHI | GCGC | 2 cut(s) | 542, 1058 |
| BstKTI | GATC | 5 cut(s) | 99, 590, 801, 921, 1242 |
| BstMAI | GTCTC | 3 cut(s) | 15, 263, 332 |
| BstMBI | GATC | 5 cut(s) | 96, 587, 798, 918, 1239 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 89, 389, 398, 512, 759, 768, 1053 |
| BstNI | CCWGG | 2 cut(s) | 537, 620 |
| BstSCI | CCNGG | 4 cut(s) | 403, 535, 618, 1145 |
| BstV1I | GCAGC | 3 cut(s) | 801, 849, 855 |
| BstX2I | RGATCY | 1 cut(s) | 96 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 460 |
| BstYI | RGATCY | 1 cut(s) | 96 |
| BtgI | CCRYGG | 3 cut(s) | 459, 525, 1020 |
| BtgZI | GCGATG | 2 cut(s) | 193, 739 |
| BtrI | CACGTC | 1 cut(s) | 379 |
| BtsCI | GGATG | 4 cut(s) | 69, 122, 250, 744 |
| BtsIMutI | CAGTG | 5 cut(s) | 306, 612, 801, 835, 1249 |
| BveI | ACCTGC | 1 cut(s) | 455 |
| Cac8I | GCNNGC | 6 cut(s) | 363, 517, 760, 862, 866, 994 |
| CaiI | CAGNNNCTG | 2 cut(s) | 272, 320 |
| CfoI | GCGC | 2 cut(s) | 542, 1058 |
| Cfr10I | RCCGGY | 1 cut(s) | 767 |
| Cfr13I | GGNCC | 9 cut(s) | 58, 382, 413, 467, 491, 690, 1096, 1143, 1201 |
| Csp6I | GTAC | 4 cut(s) | 311, 435, 1104, 1153 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 211, 246 |
| CviAII | CATG | 5 cut(s) | 568, 1021, 1043, 1052, 1061 |
| CviQI | GTAC | 4 cut(s) | 311, 435, 1104, 1153 |
| DdeI | CTNAG | 3 cut(s) | 88, 162, 342 |
| DinI | GGCGCC | 1 cut(s) | 541 |
| DpnI | GATC | 5 cut(s) | 98, 589, 800, 920, 1241 |
| DpnII | GATC | 5 cut(s) | 96, 587, 798, 918, 1239 |
| DraIII | CACNNNGTG | 1 cut(s) | 407 |
| EaeI | YGGCCR | 3 cut(s) | 3, 193, 1176 |
| Eco130I | CCWWGG | 3 cut(s) | 73, 654, 1020 |
| Eco147I | AGGCCT | 1 cut(s) | 762 |
| Eco47I | GGWCC | 7 cut(s) | 58, 413, 467, 690, 1096, 1143, 1201 |
| Eco88I | CYCGRG | 1 cut(s) | 335 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 104, 1193 |
| EcoO109I | RGGNCCY | 3 cut(s) | 413, 467, 690 |
| EcoRII | CCWGG | 2 cut(s) | 535, 618 |
| EcoT14I | CCWWGG | 3 cut(s) | 73, 654, 1020 |
| EgeI | GGCGCC | 1 cut(s) | 541 |
| EheI | GGCGCC | 1 cut(s) | 541 |
| ErhI | CCWWGG | 3 cut(s) | 73, 654, 1020 |
| FaeI | CATG | 5 cut(s) | 571, 1024, 1046, 1055, 1064 |
| FaqI | GGGAC | 4 cut(s) | 131, 434, 703, 1156 |
| FatI | CATG | 5 cut(s) | 567, 1020, 1042, 1051, 1060 |
| FauI | CCCGC | 3 cut(s) | 211, 223, 677 |
| FokI | GGATG | 4 cut(s) | 56, 109, 257, 731 |
| FspBI | CTAG | 3 cut(s) | 62, 93, 581 |
| GlaI | GCGC | 2 cut(s) | 541, 1057 |
| GsaI | CCCAGC | 2 cut(s) | 396, 953 |
| GsuI | CTGGAG | 2 cut(s) | 872, 897 |
| HaeII | RGCGCY | 1 cut(s) | 543 |
| HapII | CCGG | 3 cut(s) | 405, 768, 1146 |
| HhaI | GCGC | 2 cut(s) | 542, 1058 |
| Hin1I | GRCGYC | 1 cut(s) | 540 |
| Hin1II | CATG | 5 cut(s) | 571, 1024, 1046, 1055, 1064 |
| Hin6I | GCGC | 2 cut(s) | 540, 1056 |
| HinP1I | GCGC | 2 cut(s) | 540, 1056 |
| HincII | GTYRAC | 1 cut(s) | 699 |
| HindII | GTYRAC | 1 cut(s) | 699 |
| HindIII | AAGCTT | 2 cut(s) | 893, 1084 |
| HinfI | GANTC | 1 cut(s) | 254 |
| HpaII | CCGG | 3 cut(s) | 405, 768, 1146 |
| HphI | GGTGA | 8 cut(s) | 295, 420, 474, 757, 766, 922, 928, 1021 |
| Hpy166II | GTNNAC | 4 cut(s) | 699, 1099, 1225, 1249 |
| Hpy188I | TCNGA | 3 cut(s) | 165, 605, 1258 |
| Hpy188III | TCNNGA | 5 cut(s) | 266, 374, 802, 889, 1196 |
| Hpy8I | GTNNAC | 4 cut(s) | 699, 1099, 1225, 1249 |
| Hpy99I | CGWCG | 2 cut(s) | 926, 929 |
| HpyAV | CCTTC | 4 cut(s) | 571, 859, 932, 1199 |
| HpyCH4III | ACNGT | 9 cut(s) | 229, 310, 358, 526, 634, 796, 916, 1033, 1103 |
| HpyCH4IV | ACGT | 2 cut(s) | 378, 822 |
| HpyCH4V | TGCA | 3 cut(s) | 571, 1042, 1237 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 89, 389, 398, 512, 759, 768, 1053 |
| HpyF3I | CTNAG | 3 cut(s) | 88, 162, 342 |
| HpySE526I | ACGT | 2 cut(s) | 378, 822 |
| Hsp92I | GRCGYC | 1 cut(s) | 540 |
| Hsp92II | CATG | 5 cut(s) | 571, 1024, 1046, 1055, 1064 |
| HspAI | GCGC | 2 cut(s) | 540, 1056 |
| KasI | GGCGCC | 1 cut(s) | 539 |
| KflI | GGGWCCC | 1 cut(s) | 690 |
| Kzo9I | GATC | 5 cut(s) | 96, 587, 798, 918, 1239 |
| LmnI | GCTCC | 2 cut(s) | 524, 878 |
| Lsp1109I | GCAGC | 3 cut(s) | 801, 849, 855 |
| LweI | GCATC | 3 cut(s) | 131, 238, 762 |
| MaeI | CTAG | 3 cut(s) | 62, 93, 581 |
| MaeII | ACGT | 2 cut(s) | 378, 822 |
| MaeIII | GTNAC | 5 cut(s) | 301, 421, 610, 853, 910 |
| MalI | GATC | 5 cut(s) | 98, 589, 800, 920, 1241 |
| MboI | GATC | 5 cut(s) | 96, 587, 798, 918, 1239 |
| MboII | GAAGA | 2 cut(s) | 556, 664 |
| MflI | RGATCY | 1 cut(s) | 96 |
| MlsI | TGGCCA | 2 cut(s) | 5, 195 |
| MluCI | AATT | 1 cut(s) | 261 |
| MluNI | TGGCCA | 2 cut(s) | 5, 195 |
| Mly113I | GGCGCC | 1 cut(s) | 540 |
| MlyI | GAGTC | 1 cut(s) | 248 |
| MmeI | TCCRAC | 1 cut(s) | 981 |
| Mox20I | TGGCCA | 2 cut(s) | 5, 195 |
| MscI | TGGCCA | 2 cut(s) | 5, 195 |
| MseI | TTAA | 1 cut(s) | 713 |
| MslI | CAYNNNNRTG | 3 cut(s) | 458, 1130, 1232 |
| Msp20I | TGGCCA | 2 cut(s) | 5, 195 |
| MspA1I | CMGCKG | 3 cut(s) | 684, 810, 868 |
| MspI | CCGG | 3 cut(s) | 405, 768, 1146 |
| MspR9I | CCNGG | 4 cut(s) | 405, 537, 620, 1147 |
| MvaI | CCWGG | 2 cut(s) | 537, 620 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 89, 389, 398, 512, 759, 768, 1053 |
| NarI | GGCGCC | 1 cut(s) | 540 |
| NciI | CCSGG | 2 cut(s) | 405, 1147 |
| NcoI | CCATGG | 1 cut(s) | 1020 |
| NdeII | GATC | 5 cut(s) | 96, 587, 798, 918, 1239 |
| NlaIII | CATG | 5 cut(s) | 571, 1024, 1046, 1055, 1064 |
| NmuCI | GTSAC | 5 cut(s) | 301, 421, 610, 853, 910 |
| OliI | CACNNNNGTG | 1 cut(s) | 458 |
| PceI | AGGCCT | 1 cut(s) | 762 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 723, 933 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1021 |
| PleI | GAGTC | 1 cut(s) | 248 |
| PluTI | GGCGCC | 1 cut(s) | 543 |
| PpsI | GAGTC | 1 cut(s) | 248 |
| PpuMI | RGGWCCY | 3 cut(s) | 413, 467, 690 |
| Psp1406I | AACGTT | 1 cut(s) | 822 |
| Psp5II | RGGWCCY | 3 cut(s) | 413, 467, 690 |
| Psp6I | CCWGG | 2 cut(s) | 535, 618 |
| PspFI | CCCAGC | 2 cut(s) | 392, 949 |
| PspGI | CCWGG | 2 cut(s) | 535, 618 |
| PspPI | GGNCC | 9 cut(s) | 58, 382, 413, 467, 491, 690, 1096, 1143, 1201 |
| PspPPI | RGGWCCY | 3 cut(s) | 413, 467, 690 |
| PstNI | CAGNNNCTG | 2 cut(s) | 272, 320 |
| PsuI | RGATCY | 1 cut(s) | 96 |
| PvuII | CAGCTG | 1 cut(s) | 868 |
| RsaI | GTAC | 4 cut(s) | 312, 436, 1105, 1154 |
| RsaNI | GTAC | 4 cut(s) | 311, 435, 1104, 1153 |
| RseI | CAYNNNNRTG | 3 cut(s) | 458, 1130, 1232 |
| SaqAI | TTAA | 1 cut(s) | 713 |
| Sau3AI | GATC | 5 cut(s) | 96, 587, 798, 918, 1239 |
| Sau96I | GGNCC | 9 cut(s) | 58, 382, 413, 467, 491, 690, 1096, 1143, 1201 |
| SchI | GAGTC | 1 cut(s) | 248 |
| ScrFI | CCNGG | 4 cut(s) | 405, 537, 620, 1147 |
| SfaNI | GCATC | 3 cut(s) | 131, 238, 762 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 768 |
| SfoI | GGCGCC | 1 cut(s) | 541 |
| SinI | GGWCC | 7 cut(s) | 58, 413, 467, 690, 1096, 1143, 1201 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 458, 1130, 1232 |
| SmlI | CTYRAG | 1 cut(s) | 35 |
| SmoI | CTYRAG | 1 cut(s) | 35 |
| Sse9I | AATT | 1 cut(s) | 261 |
| SseBI | AGGCCT | 1 cut(s) | 762 |
| SspDI | GGCGCC | 1 cut(s) | 539 |
| SspMI | CTAG | 3 cut(s) | 62, 93, 581 |
| StuI | AGGCCT | 1 cut(s) | 762 |
| StyD4I | CCNGG | 4 cut(s) | 403, 535, 618, 1145 |
| StyI | CCWWGG | 3 cut(s) | 73, 654, 1020 |
| TaaI | ACNGT | 9 cut(s) | 229, 310, 358, 526, 634, 796, 916, 1033, 1103 |
| TaiI | ACGT | 2 cut(s) | 381, 825 |
| TaqI | TCGA | 2 cut(s) | 726, 921 |
| TaqII | GACCGA | 1 cut(s) | 950 |
| TasI | AATT | 1 cut(s) | 261 |
| TauI | GCSGC | 7 cut(s) | 401, 509, 512, 515, 813, 837, 1178 |
| Tru1I | TTAA | 1 cut(s) | 713 |
| Tru9I | TTAA | 1 cut(s) | 713 |
| TscAI | CASTG | 5 cut(s) | 313, 619, 801, 835, 1249 |
| TseFI | GTSAC | 5 cut(s) | 301, 421, 610, 853, 910 |
| TseI | GCWGC | 3 cut(s) | 789, 837, 868 |
| Tsp45I | GTSAC | 5 cut(s) | 301, 421, 610, 853, 910 |
| TspDTI | ATGAA | 2 cut(s) | 556, 1181 |
| TspGWI | ACGGA | 1 cut(s) | 254 |
| TspRI | CASTG | 5 cut(s) | 313, 619, 801, 835, 1249 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1021 |
| VpaK11BI | GGWCC | 7 cut(s) | 58, 413, 467, 690, 1096, 1143, 1201 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 104, 1193 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 376, 872 |
| XspI | CTAG | 3 cut(s) | 62, 93, 581 |