Rw1G029300

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016187
Physical Location & Seq
Forward (+)
56988570 .. 56989811
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G029300.1

Sequence Viewer

Length: 1242 bp
ATGACCTCCAAAGCAGTCAAGGCCACCTTCTACATTTTATTCCTAGCCATCATCACACTTGGATATGCCAACTCAGCTAGGATCCTTGATGAAGCAACTAATCCCCTCCCGACAACTGTCCAGGCCTCAAACCCGATAAATCCCACCACTCCGTTGCCAAGTGGCCAAATCCCTGCCACCACAACCGTGGATGACAACAAGGACGACATGGAGGCACCAATTCCAGACGTCACACCACCCGTTGATGTGGCATCACCAATAGTTGCTCCTGTCACCCCCACAATGCCAGAAGCCGATGTCACACCACCCGCTGATGTGGCATCACCAATAGTCGCTCCTGTTACCCCCGAGGTGCCAGAAGCCGACTTACCCCAACCCGAAGAACCTGACACTACTATTGTGCCAACACCCGTAGCGGGCCCCGTCATCCCCTCCGCAACCGCGCCAATCCCGGGCCCAGCCATCCCGACACCAAAAGTGCCGCTCCCAGTTGCAACACCCGTGGCGGGGCCCGCTACCCCCGAAGCGAAACCGCATTTGGCCTTCTTCATGCACGACATTCTTGGAGGATCCCAACCGTCCGTTCGGGTGGTGACCGGGCTCATCGCCAGCACGGTGGCCAACGTCGCCTTCTCGAAACCCAACAACAACATCTTCCCCGTCAGCGGTGGGACCCCCCTAAGCAACAACAACCTCAACGGCTTCGTCAACAACAACCGCAACAACCTCCACTCCATCGCCGGCCTCACCGGCACCACCGGCCTCACCAACTCCCAATCCAGCACTGTCATACAAAACTCCGGGAACAACAACATCGTCGACGGCGGCGACAACCAGCCTTTCGTCACCGCCGGCCAGCTCCCCAACGGTGCCACCCTCCAGAAGCTCATGTTCGGCTCCGTCACCGTCATCGACGACCAACTGACCGAAGGACAGGAGCTCGGCTCCGCCGTGCTCGGAAAAGCTCAGGGTTTCTACTTGGCCAGCTCTTTGGACGGAAACAGCCACACAATGGCGTTCACTGTTCTCATGCACGGCGAGCATGACGTGGAGGACACCATCAGCTTCTTCGGGGTCCACCGCACTGCGGCGCCGGTGTCGCACATTGCTGTGATCGGCGGGACCGGGAAGTACGAGAATGCGAATGGTTATGGGGCCATTGAGAGCCTTCATCAGGAGGACCAGCACACAACGGACGGTGTGGATACCATAATGCAGATCAGTGTTTACCTCTCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

413

Amino Acids

42.67

Weight (kDa)

4.43

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 271 - 410 3.3e-27 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39430 AT3G55230
fragaria_vesca FvH4_7g21000
malus_domestica MD01G1119900.v1.1 MD07G1189200.v1.1
prunus_persica Prupe.2G226700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365391
rosa_laevigata RLG00000027426
rosa_multiflora Rmu_sc0008527.1_g000004
rosa_roxburghii Rroxscaffold_4G00291260
rosa_rugosa Rorug01G0321600
rosa_samantha Rh1AG330500 Rh1BG292000 Rh1DG323600
rosa_wichuraiana Rw1G029300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 231
AccB1I GGYRCC 5 cut(s) 214, 352, 752, 869, 1090
AccB7I CCANNNNNTGG 1 cut(s) 1012
AccBSI CCGCTC 1 cut(s) 484
AccI GTMKAC 1 cut(s) 819
AccII CGCG 1 cut(s) 443
AclWI GGATC 4 cut(s) 76, 89, 564, 577
AcoI YGGCCR 4 cut(s) 163, 618, 853, 981
AcyI GRCGYC 2 cut(s) 228, 1091
AfaI GTAC 1 cut(s) 1133
AfiI CCNNNNNNNGG 8 cut(s) 416, 452, 506, 507, 665, 1012, 1087, 1174
AjiI CACGTC 1 cut(s) 1048
AjnI CCWGG 1 cut(s) 120
AjuI GAANNNNNNNTTGG 6 cut(s) 521, 553, 567, 599, 614, 646
AleI CACNNNNGTG 1 cut(s) 185
AluBI AGCT 7 cut(s) 77, 859, 886, 940, 965, 987, 1065
AluI AGCT 7 cut(s) 77, 859, 886, 940, 965, 987, 1065
Alw21I GWGCWC 2 cut(s) 942, 957
AlwI GGATC 4 cut(s) 76, 89, 564, 577
Ama87I CYCGRG 2 cut(s) 347, 451
ApaI GGGCCC 3 cut(s) 422, 458, 513
AspLEI GCGC 2 cut(s) 445, 1093
AsuC2I CCSGG 5 cut(s) 452, 453, 598, 802, 1126
AsuHPI GGTGA 8 cut(s) 246, 265, 315, 604, 739, 757, 838, 895
AvaI CYCGRG 2 cut(s) 347, 451
AvaII GGWCC 4 cut(s) 672, 1075, 1122, 1180
BaeGI GKGCMC 3 cut(s) 422, 458, 513
BalI TGGCCA 3 cut(s) 165, 620, 983
BamHI GGATCC 2 cut(s) 81, 569
BanI GGYRCC 5 cut(s) 214, 352, 752, 869, 1090
BanII GRGCYC 5 cut(s) 422, 458, 513, 603, 942
BarI GAAGNNNNNNTAC 2 cut(s) 351, 383
Bbv12I GWGCWC 2 cut(s) 942, 957
BccI CCATC 4 cut(s) 56, 470, 743, 1067
BceAI ACGGC 4 cut(s) 715, 838, 935, 1051
BciT130I CCWGG 1 cut(s) 122
BciVI GTATCC 1 cut(s) 1198
BcnI CCSGG 5 cut(s) 452, 453, 598, 802, 1126
BfaI CTAG 2 cut(s) 44, 78
BfoI RGCGCY 1 cut(s) 1094
BfuI GTATCC 1 cut(s) 1198
BglI GCCNNNNNGGC 1 cut(s) 750
BisI GCNGC 3 cut(s) 482, 826, 1089
BlsI GCNGC 3 cut(s) 483, 827, 1090
Bme1390I CCNGG 6 cut(s) 122, 452, 453, 598, 802, 1126
Bme18I GGWCC 4 cut(s) 672, 1075, 1122, 1180
BmeT110I CYCGRG 2 cut(s) 347, 451
BmgBI CACGTC 1 cut(s) 1048
BmrFI CCNGG 6 cut(s) 122, 452, 453, 598, 802, 1126
BmrI ACTGGG 1 cut(s) 482
BmsI GCATC 2 cut(s) 260, 329
BmuI ACTGGG 1 cut(s) 482
BoxI GACNNNNGTC 1 cut(s) 116
BplI GAGNNNNNCTC 2 cut(s) 929, 961
BpmI CTGGAG 1 cut(s) 863
Bpu10I CCTNAGC 2 cut(s) 680, 966
BpuMI CCSGG 5 cut(s) 452, 453, 598, 802, 1126
BsaHI GRCGYC 2 cut(s) 228, 1091
BsaJI CCNNGG 4 cut(s) 186, 348, 451, 501
BsaXI ACNNNNNCTCC 2 cut(s) 716, 746
Bsc4I CCNNNNNNNGG 8 cut(s) 416, 452, 506, 507, 665, 1012, 1087, 1174
Bse118I RCCGGY 5 cut(s) 740, 749, 758, 851, 1093
Bse1I ACTGG 1 cut(s) 488
Bse3DI GCAATG 1 cut(s) 1104
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 4 cut(s) 186, 348, 451, 501
BseGI GGATG 3 cut(s) 196, 426, 462
BseLI CCNNNNNNNGG 8 cut(s) 416, 452, 506, 507, 665, 1012, 1087, 1174
BseMI GCAATG 1 cut(s) 1104
BseMII CTCAG 3 cut(s) 87, 980, 1227
BseNI ACTGG 1 cut(s) 488
BseSI GKGCMC 3 cut(s) 422, 458, 513
BseYI CCCAGC 1 cut(s) 457
Bsh1236I CGCG 1 cut(s) 443
BshNI GGYRCC 5 cut(s) 214, 352, 752, 869, 1090
BsiHKAI GWGCWC 2 cut(s) 942, 957
BsiHKCI CYCGRG 2 cut(s) 347, 451
BsiSI CCGG 9 cut(s) 452, 597, 741, 750, 759, 801, 852, 1094, 1125
BslFI GGGAC 2 cut(s) 685, 1135
BslI CCNNNNNNNGG 8 cut(s) 416, 452, 506, 507, 665, 1012, 1087, 1174
BsmFI GGGAC 2 cut(s) 685, 1135
BsmI GAATGC 1 cut(s) 1144
BsoBI CYCGRG 2 cut(s) 347, 451
Bsp120I GGGCCC 3 cut(s) 418, 454, 509
Bsp1286I GDGCHC 6 cut(s) 422, 458, 513, 603, 942, 957
Bsp143I GATC 4 cut(s) 81, 569, 1113, 1218
BspCNI CTCAG 3 cut(s) 86, 979, 1228
BspFNI CGCG 1 cut(s) 443
BspPI GGATC 4 cut(s) 76, 89, 564, 577
BspT107I GGYRCC 5 cut(s) 214, 352, 752, 869, 1090
BsrBI CCGCTC 1 cut(s) 484
BsrDI GCAATG 1 cut(s) 1104
BsrFI RCCGGY 5 cut(s) 740, 749, 758, 851, 1093
BsrI ACTGG 1 cut(s) 488
BssAI RCCGGY 5 cut(s) 740, 749, 758, 851, 1093
BssECI CCNNGG 4 cut(s) 186, 348, 451, 501
BssMI GATC 4 cut(s) 81, 569, 1113, 1218
BssNI GRCGYC 2 cut(s) 228, 1091
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 9 cut(s) 118, 187, 579, 616, 787, 869, 907, 1024, 1199
BstACI GRCGYC 2 cut(s) 228, 1091
BstC8I GCNNGC 8 cut(s) 418, 513, 610, 742, 853, 857, 985, 1040
BstDEI CTNAG 4 cut(s) 73, 680, 966, 1236
BstDSI CCRYGG 2 cut(s) 186, 501
BstEII GGTNACC 1 cut(s) 592
BstENI CCTNNNNNAGG 1 cut(s) 1172
BstF5I GGATG 3 cut(s) 196, 426, 462
BstFNI CGCG 1 cut(s) 443
BstH2I RGCGCY 1 cut(s) 1094
BstHHI GCGC 2 cut(s) 445, 1093
BstKTI GATC 4 cut(s) 84, 572, 1116, 1221
BstMBI GATC 4 cut(s) 81, 569, 1113, 1218
BstMWI GCNNNNNNNGC 9 cut(s) 20, 74, 317, 512, 626, 750, 759, 1039, 1099
BstNI CCWGG 1 cut(s) 122
BstPAI GACNNNNGTC 1 cut(s) 116
BstPI GGTNACC 1 cut(s) 592
BstSCI CCNGG 6 cut(s) 120, 450, 451, 596, 800, 1124
BstSLI GKGCMC 3 cut(s) 422, 458, 513
BstUI CGCG 1 cut(s) 443
BstX2I RGATCY 2 cut(s) 81, 569
BstXI CCANNNNNNTGG 3 cut(s) 187, 616, 991
BstYI RGATCY 2 cut(s) 81, 569
BsuI GTATCC 1 cut(s) 1198
BtgI CCRYGG 2 cut(s) 186, 501
BtgZI GCGATG 2 cut(s) 589, 721
BtrI CACGTC 1 cut(s) 1048
BtsCI GGATG 3 cut(s) 196, 426, 462
BtsI GCAGTG 1 cut(s) 1083
BtsIMutI CAGTG 4 cut(s) 783, 1020, 1083, 1228
Cac8I GCNNGC 8 cut(s) 418, 513, 610, 742, 853, 857, 985, 1040
CfoI GCGC 2 cut(s) 445, 1093
Cfr10I RCCGGY 5 cut(s) 740, 749, 758, 851, 1093
Cfr9I CCCGGG 1 cut(s) 451
Csp6I GTAC 1 cut(s) 1132
CspCI CAANNNNNGTGG 2 cut(s) 483, 518
CviAII CATG 5 cut(s) 208, 550, 889, 1030, 1043
CviQI GTAC 1 cut(s) 1132
DdeI CTNAG 4 cut(s) 73, 680, 966, 1236
DinI GGCGCC 1 cut(s) 1092
DpnI GATC 4 cut(s) 83, 571, 1115, 1220
DpnII GATC 4 cut(s) 81, 569, 1113, 1218
EaeI YGGCCR 4 cut(s) 163, 618, 853, 981
EciI GGCGGA 1 cut(s) 937
Ecl136II GAGCTC 1 cut(s) 940
Eco147I AGGCCT 1 cut(s) 125
Eco24I GRGCYC 5 cut(s) 422, 458, 513, 603, 942
Eco47I GGWCC 4 cut(s) 672, 1075, 1122, 1180
Eco53kI GAGCTC 1 cut(s) 940
Eco88I CYCGRG 2 cut(s) 347, 451
Eco91I GGTNACC 1 cut(s) 592
EcoICRI GAGCTC 1 cut(s) 940
EcoNI CCTNNNNNAGG 1 cut(s) 1172
EcoO109I RGGNCCY 3 cut(s) 419, 509, 672
EcoO65I GGTNACC 1 cut(s) 592
EcoRII CCWGG 1 cut(s) 120
EcoT38I GRGCYC 5 cut(s) 422, 458, 513, 603, 942
EgeI GGCGCC 1 cut(s) 1092
EheI GGCGCC 1 cut(s) 1092
FaeI CATG 5 cut(s) 211, 553, 892, 1033, 1046
FaiI YATR 9 cut(s) 66, 209, 551, 791, 890, 1031, 1044, 1152, 1211
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 2 cut(s) 685, 1135
FatI CATG 5 cut(s) 207, 549, 888, 1029, 1042
FauI CCCGC 5 cut(s) 316, 409, 499, 520, 1112
FblI GTMKAC 1 cut(s) 819
Fnu4HI GCNGC 3 cut(s) 482, 826, 1089
FokI GGATG 3 cut(s) 203, 413, 449
FriOI GRGCYC 5 cut(s) 422, 458, 513, 603, 942
Fsp4HI GCNGC 3 cut(s) 482, 826, 1089
FspBI CTAG 2 cut(s) 44, 78
GlaI GCGC 2 cut(s) 444, 1092
GluI GCNGC 3 cut(s) 482, 826, 1089
GsaI CCCAGC 1 cut(s) 461
GsuI CTGGAG 1 cut(s) 863
HaeII RGCGCY 1 cut(s) 1094
HapII CCGG 9 cut(s) 452, 597, 741, 750, 759, 801, 852, 1094, 1125
HhaI GCGC 2 cut(s) 445, 1093
Hin1I GRCGYC 2 cut(s) 228, 1091
Hin1II CATG 5 cut(s) 211, 553, 892, 1033, 1046
Hin6I GCGC 2 cut(s) 443, 1091
HinP1I GCGC 2 cut(s) 443, 1091
HincII GTYRAC 2 cut(s) 709, 820
HindII GTYRAC 2 cut(s) 709, 820
HpaII CCGG 9 cut(s) 452, 597, 741, 750, 759, 801, 852, 1094, 1125
HphI GGTGA 8 cut(s) 246, 265, 315, 604, 739, 757, 838, 895
Hpy166II GTNNAC 5 cut(s) 709, 820, 1020, 1078, 1228
Hpy188I TCNGA 2 cut(s) 959, 1237
Hpy188III TCNNGA 6 cut(s) 109, 224, 466, 634, 880, 1175
Hpy8I GTNNAC 5 cut(s) 709, 820, 1020, 1078, 1228
Hpy99I CGWCG 4 cut(s) 629, 821, 824, 917
HpyAV CCTTC 5 cut(s) 37, 553, 640, 923, 1178
HpyCH4III ACNGT 9 cut(s) 118, 187, 579, 616, 787, 869, 907, 1024, 1199
HpyCH4IV ACGT 3 cut(s) 228, 624, 1047
HpyCH4V TGCA 4 cut(s) 494, 553, 1033, 1216
HpyF10VI GCNNNNNNNGC 9 cut(s) 20, 74, 317, 512, 626, 750, 759, 1039, 1099
HpyF3I CTNAG 4 cut(s) 73, 680, 966, 1236
HpySE526I ACGT 3 cut(s) 228, 624, 1047
Hsp92I GRCGYC 2 cut(s) 228, 1091
Hsp92II CATG 5 cut(s) 211, 553, 892, 1033, 1046
HspAI GCGC 2 cut(s) 443, 1091
KasI GGCGCC 1 cut(s) 1090
KflI GGGWCCC 1 cut(s) 672
KroI GCCGGC 2 cut(s) 740, 851
KroNI GCCGGC 2 cut(s) 742, 853
Kzo9I GATC 4 cut(s) 81, 569, 1113, 1218
LmnI GCTCC 7 cut(s) 271, 340, 489, 864, 902, 937, 950
LweI GCATC 2 cut(s) 260, 329
MaeI CTAG 2 cut(s) 44, 78
MaeII ACGT 3 cut(s) 228, 624, 1047
MaeIII GTNAC 7 cut(s) 229, 271, 298, 340, 592, 844, 901
MalI GATC 4 cut(s) 83, 571, 1115, 1220
MbiI CCGCTC 1 cut(s) 484
MboI GATC 4 cut(s) 81, 569, 1113, 1218
MboII GAAGA 4 cut(s) 392, 538, 646, 1060
MflI RGATCY 2 cut(s) 81, 569
MhlI GDGCHC 6 cut(s) 422, 458, 513, 603, 942, 957
MlsI TGGCCA 3 cut(s) 165, 620, 983
MluCI AATT 1 cut(s) 219
MluNI TGGCCA 3 cut(s) 165, 620, 983
Mly113I GGCGCC 1 cut(s) 1091
Mox20I TGGCCA 3 cut(s) 165, 620, 983
MroNI GCCGGC 2 cut(s) 740, 851
MscI TGGCCA 3 cut(s) 165, 620, 983
MslI CAYNNNNRTG 2 cut(s) 185, 1109
Msp20I TGGCCA 3 cut(s) 165, 620, 983
MspA1I CMGCKG 2 cut(s) 311, 666
MspI CCGG 9 cut(s) 452, 597, 741, 750, 759, 801, 852, 1094, 1125
MspR9I CCNGG 6 cut(s) 122, 452, 453, 598, 802, 1126
Mva1269I GAATGC 1 cut(s) 1144
MvaI CCWGG 1 cut(s) 122
MvnI CGCG 1 cut(s) 443
MwoI GCNNNNNNNGC 9 cut(s) 20, 74, 317, 512, 626, 750, 759, 1039, 1099
NaeI GCCGGC 2 cut(s) 742, 853
NarI GGCGCC 1 cut(s) 1091
NciI CCSGG 5 cut(s) 452, 453, 598, 802, 1126
NdeII GATC 4 cut(s) 81, 569, 1113, 1218
NgoMIV GCCGGC 2 cut(s) 740, 851
NlaIII CATG 5 cut(s) 211, 553, 892, 1033, 1046
NmeAIII GCCGAG 1 cut(s) 921
NmuCI GTSAC 6 cut(s) 229, 271, 298, 592, 844, 901
OliI CACNNNNGTG 1 cut(s) 185
PceI AGGCCT 1 cut(s) 125
PcsI WCGNNNNNNNCGW 2 cut(s) 825, 948
PctI GAATGC 1 cut(s) 1144
PdiI GCCGGC 2 cut(s) 742, 853
PflMI CCANNNNNTGG 1 cut(s) 1012
PfoI TCCNGGA 1 cut(s) 800
PkrI GCNGC 3 cut(s) 483, 827, 1090
PluTI GGCGCC 1 cut(s) 1094
PpuMI RGGWCCY 1 cut(s) 672
PshAI GACNNNNGTC 1 cut(s) 116
Psp124BI GAGCTC 1 cut(s) 942
Psp5II RGGWCCY 1 cut(s) 672
Psp6I CCWGG 1 cut(s) 120
PspEI GGTNACC 1 cut(s) 592
PspFI CCCAGC 1 cut(s) 457
PspGI CCWGG 1 cut(s) 120
PspOMI GGGCCC 3 cut(s) 418, 454, 509
PspPPI RGGWCCY 1 cut(s) 672
PsuI RGATCY 2 cut(s) 81, 569
RsaI GTAC 1 cut(s) 1133
RsaNI GTAC 1 cut(s) 1132
RseI CAYNNNNRTG 2 cut(s) 185, 1109
SacI GAGCTC 1 cut(s) 942
SalI GTCGAC 1 cut(s) 818
SatI GCNGC 3 cut(s) 482, 826, 1089
Sau3AI GATC 4 cut(s) 81, 569, 1113, 1218
ScrFI CCNGG 6 cut(s) 122, 452, 453, 598, 802, 1126
SduI GDGCHC 6 cut(s) 422, 458, 513, 603, 942, 957
SfaNI GCATC 2 cut(s) 260, 329
SfoI GGCGCC 1 cut(s) 1092
SgrAI CRCCGGYG 1 cut(s) 1093
SgrDI CGTCGACG 1 cut(s) 818
SinI GGWCC 4 cut(s) 672, 1075, 1122, 1180
SmaI CCCGGG 1 cut(s) 453
SmiMI CAYNNNNRTG 2 cut(s) 185, 1109
Sse9I AATT 1 cut(s) 219
SseBI AGGCCT 1 cut(s) 125
SspDI GGCGCC 1 cut(s) 1090
SspMI CTAG 2 cut(s) 44, 78
SstI GAGCTC 1 cut(s) 942
StuI AGGCCT 1 cut(s) 125
StyD4I CCNGG 6 cut(s) 120, 450, 451, 596, 800, 1124
TaaI ACNGT 9 cut(s) 118, 187, 579, 616, 787, 869, 907, 1024, 1199
TaiI ACGT 3 cut(s) 231, 627, 1050
TaqI TCGA 3 cut(s) 635, 819, 912
TaqII GACCGA 1 cut(s) 941
TasI AATT 1 cut(s) 219
TauI GCSGC 3 cut(s) 484, 828, 1091
TscAI CASTG 4 cut(s) 790, 1027, 1090, 1228
TseFI GTSAC 6 cut(s) 229, 271, 298, 592, 844, 901
Tsp45I GTSAC 6 cut(s) 229, 271, 298, 592, 844, 901
TspDTI ATGAA 3 cut(s) 105, 538, 1160
TspGWI ACGGA 5 cut(s) 141, 571, 889, 1011, 1208
TspMI CCCGGG 1 cut(s) 451
TspRI CASTG 4 cut(s) 790, 1027, 1090, 1228
Van91I CCANNNNNTGG 1 cut(s) 1012
VpaK11BI GGWCC 4 cut(s) 672, 1075, 1122, 1180
XagI CCTNNNNNAGG 1 cut(s) 1172
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XmaI CCCGGG 1 cut(s) 451
XmiI GTMKAC 1 cut(s) 819
XspI CTAG 2 cut(s) 44, 78
ZraI GACGTC 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.