Rroxscaffold_4G00291260

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016187
Physical Location & Seq
Reverse (-)
11713667 .. 11714927
1261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00291260.1

Sequence Viewer

Length: 1200 bp
ATGACCTCCAAAGCAGTCAAGGCCACCTTCTACATTTTATTTCTAGCCATCATTACACTTGGATATGCCAACTCAGCTAGGATCCTTGATGAAGCAACTAATCCCCTCCCGACAACTGTCCAGGCTTCAAACCCAATAGATCCTACCACTCCGTTGCCAAGTGGCCAAATCCCTGCCACCACAACCGTGGATGACAACAAGGACGACACTGAGGCACCAATTCCAGACACTGATGCCCCTGTTGATGATGTCCAACCACCCGTTGATGTGGCATCACCAATAGTTGCTCCTCTCACCCCGCGGTGCCAGAAGCCGATAAGCCGACTCACCCCAACCCGAAGAACCCGACACTACTATCGTGCCAACACCCGTAGCGGGATCCATCATCCCCTCCGCAACCGCGCCAATCCCTGGCCCAGCCATCGCGACACCAAAAGTGCCGCTTCCAGTTGCAACACCCGTGGCGGGGCCTGCTACCACCGAAGCGAAACCGCATTTGGCCTTCTTCATGCACGACATTCTTGGAGGATCCCAACCGTCCGTTCGGGTGGTGACCGGGCTCATCGCCAGCACGGTGGCCAACGTCGCCTTCTCGAAACCCAACAACAACATCTTCCCGGTCAGCGGTGGGACCCCCTAAACAACAACAACCTCAACGGCTTCCTCGACAACAACCGCAACAACCTCCACTCCATCGCCGGCCTCACCGGCACCACCGGCCTCACCAACTCCCAATCCAGCACTGTCATCCAAAACTCCGGCAACAACAACATAGTCAACGGCGGCGACAACCAGCCCTTCGTCACAGCCGGCCAGCTCCCCAACGGCGCCACCCTCCAGAAGCTCATGTTCGGCTCCGTCACCGTCATCGACGACCAACTGACTGAAGGACAGGAGCTCGGCTCCGCCGTGCTCGGAAAAGCTCAGGGTTTCTACTTGGCCAGCTCTTTGGACGGAAACAGCCACACAATGGCGTTCACTGTTCTCATGCACGGCGAGCATGACGTGGAGGACACCATCAGCTTCTTCGGGGTCCACCGCACGGCGGCGCCGGTGTCGCACATTGCGGTGATCGGCGGGACCGGGAAGTATGAGAATGCGAATGGTTATGGGGCCATTGAGAGCCTTCATCAGGAGGACCAGCACACAACGGACGGTGTGGATACCATAATGCAGATCAGTGTTTACCTGTCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

399

Amino Acids

43.14

Weight (kDa)

6.4

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 257 - 396 2.5e-27 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39430 AT3G55230
fragaria_vesca FvH4_7g21000
malus_domestica MD01G1119900.v1.1 MD07G1189200.v1.1
prunus_persica Prupe.2G226700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365391
rosa_laevigata RLG00000027426
rosa_multiflora Rmu_sc0008527.1_g000004
rosa_roxburghii Rroxscaffold_4G00291260
rosa_rugosa Rorug01G0321600
rosa_samantha Rh1AG330500 Rh1BG292000 Rh1DG323600
rosa_wichuraiana Rw1G029300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 5 cut(s) 214, 303, 710, 827, 1048
AccB7I CCANNNNNTGG 2 cut(s) 411, 970
AccII CGCG 3 cut(s) 301, 402, 426
AclWI GGATC 7 cut(s) 76, 89, 134, 373, 386, 523, 536
AcoI YGGCCR 4 cut(s) 163, 577, 811, 939
AcuI CTGAAG 1 cut(s) 906
AcyI GRCGYC 2 cut(s) 828, 1049
AfiI CCNNNNNNNGG 9 cut(s) 375, 411, 465, 466, 624, 970, 1042, 1045, 1132
AgsI TTSAA 1 cut(s) 129
AjiI CACGTC 1 cut(s) 1006
AjnI CCWGG 2 cut(s) 120, 410
AjuI GAANNNNNNNTTGG 6 cut(s) 480, 512, 526, 558, 573, 605
AleI CACNNNNGTG 1 cut(s) 185
AluBI AGCT 7 cut(s) 77, 817, 844, 898, 923, 945, 1023
AluI AGCT 7 cut(s) 77, 817, 844, 898, 923, 945, 1023
Alw21I GWGCWC 2 cut(s) 900, 915
AlwI GGATC 7 cut(s) 76, 89, 134, 373, 386, 523, 536
AlwNI CAGNNNCTG 1 cut(s) 230
AspLEI GCGC 3 cut(s) 404, 830, 1051
AspS9I GGNCC 7 cut(s) 414, 468, 631, 1033, 1080, 1113, 1138
AsuC2I CCSGG 3 cut(s) 557, 618, 1084
AsuHPI GGTGA 8 cut(s) 267, 286, 319, 563, 697, 715, 853, 1081
AvaII GGWCC 4 cut(s) 631, 1033, 1080, 1138
BalI TGGCCA 3 cut(s) 165, 579, 941
BamHI GGATCC 3 cut(s) 81, 378, 528
BanI GGYRCC 5 cut(s) 214, 303, 710, 827, 1048
BanII GRGCYC 2 cut(s) 562, 900
Bbv12I GWGCWC 2 cut(s) 900, 915
BccI CCATC 5 cut(s) 56, 390, 429, 701, 1025
BceAI ACGGC 6 cut(s) 673, 796, 841, 893, 1009, 1059
BciT130I CCWGG 2 cut(s) 122, 412
BciVI GTATCC 1 cut(s) 1156
BcnI CCSGG 3 cut(s) 557, 618, 1084
BfaI CTAG 2 cut(s) 44, 78
BfoI RGCGCY 2 cut(s) 831, 1052
BfuI GTATCC 1 cut(s) 1156
BglI GCCNNNNNGGC 1 cut(s) 708
BisI GCNGC 3 cut(s) 441, 784, 1047
BlsI GCNGC 3 cut(s) 442, 785, 1048
Bme1390I CCNGG 5 cut(s) 122, 412, 557, 618, 1084
Bme18I GGWCC 4 cut(s) 631, 1033, 1080, 1138
BmgBI CACGTC 1 cut(s) 1006
BmgT120I GGNCC 7 cut(s) 414, 468, 631, 1033, 1080, 1113, 1138
BmrFI CCNGG 5 cut(s) 122, 412, 557, 618, 1084
BmsI GCATC 2 cut(s) 223, 281
BoxI GACNNNNGTC 1 cut(s) 116
BplI GAGNNNNNCTC 2 cut(s) 887, 919
BpmI CTGGAG 1 cut(s) 821
Bpu10I CCTNAGC 1 cut(s) 924
BpuMI CCSGG 3 cut(s) 557, 618, 1084
BsaHI GRCGYC 2 cut(s) 828, 1049
BsaJI CCNNGG 4 cut(s) 186, 299, 410, 460
BsaXI ACNNNNNCTCC 2 cut(s) 674, 704
Bsc4I CCNNNNNNNGG 9 cut(s) 375, 411, 465, 466, 624, 970, 1042, 1045, 1132
Bse118I RCCGGY 5 cut(s) 698, 707, 716, 809, 1051
Bse1I ACTGG 1 cut(s) 447
Bse3DI GCAATG 1 cut(s) 1062
BseBI CCWGG 2 cut(s) 122, 412
BseDI CCNNGG 4 cut(s) 186, 299, 410, 460
BseGI GGATG 3 cut(s) 196, 385, 747
BseLI CCNNNNNNNGG 9 cut(s) 375, 411, 465, 466, 624, 970, 1042, 1045, 1132
BseMI GCAATG 1 cut(s) 1062
BseMII CTCAG 4 cut(s) 87, 201, 938, 1185
BseNI ACTGG 1 cut(s) 447
BseRI GAGGAG 1 cut(s) 279
BseYI CCCAGC 1 cut(s) 416
Bsh1236I CGCG 3 cut(s) 301, 402, 426
BshNI GGYRCC 5 cut(s) 214, 303, 710, 827, 1048
BsiHKAI GWGCWC 2 cut(s) 900, 915
BsiSI CCGG 9 cut(s) 556, 618, 699, 708, 717, 759, 810, 1052, 1083
BslFI GGGAC 2 cut(s) 644, 1093
BslI CCNNNNNNNGG 9 cut(s) 375, 411, 465, 466, 624, 970, 1042, 1045, 1132
BsmFI GGGAC 2 cut(s) 644, 1093
BsmI GAATGC 1 cut(s) 1102
Bsp1286I GDGCHC 3 cut(s) 562, 900, 915
Bsp143I GATC 6 cut(s) 81, 139, 378, 528, 1071, 1176
Bsp68I TCGCGA 1 cut(s) 426
BspCNI CTCAG 4 cut(s) 86, 202, 937, 1186
BspFNI CGCG 3 cut(s) 301, 402, 426
BspPI GGATC 7 cut(s) 76, 89, 134, 373, 386, 523, 536
BspT107I GGYRCC 5 cut(s) 214, 303, 710, 827, 1048
BsrDI GCAATG 1 cut(s) 1062
BsrFI RCCGGY 5 cut(s) 698, 707, 716, 809, 1051
BsrI ACTGG 1 cut(s) 447
BssAI RCCGGY 5 cut(s) 698, 707, 716, 809, 1051
BssECI CCNNGG 4 cut(s) 186, 299, 410, 460
BssMI GATC 6 cut(s) 81, 139, 378, 528, 1071, 1176
BssNI GRCGYC 2 cut(s) 828, 1049
Bst2UI CCWGG 2 cut(s) 122, 412
Bst4CI ACNGT 8 cut(s) 118, 187, 538, 575, 745, 865, 982, 1157
BstACI GRCGYC 2 cut(s) 828, 1049
BstC8I GCNNGC 7 cut(s) 472, 569, 700, 811, 815, 943, 998
BstDEI CTNAG 4 cut(s) 73, 210, 924, 1194
BstDSI CCRYGG 3 cut(s) 186, 299, 460
BstEII GGTNACC 1 cut(s) 551
BstENI CCTNNNNNAGG 1 cut(s) 1130
BstF5I GGATG 3 cut(s) 196, 385, 747
BstFNI CGCG 3 cut(s) 301, 402, 426
BstH2I RGCGCY 2 cut(s) 831, 1052
BstHHI GCGC 3 cut(s) 404, 830, 1051
BstKTI GATC 6 cut(s) 84, 142, 381, 531, 1074, 1179
BstMBI GATC 6 cut(s) 81, 139, 378, 528, 1071, 1176
BstMWI GCNNNNNNNGC 8 cut(s) 20, 74, 471, 585, 708, 717, 997, 1057
BstNI CCWGG 2 cut(s) 122, 412
BstPAI GACNNNNGTC 1 cut(s) 116
BstPI GGTNACC 1 cut(s) 551
BstSCI CCNGG 5 cut(s) 120, 410, 555, 616, 1082
BstUI CGCG 3 cut(s) 301, 402, 426
BstX2I RGATCY 4 cut(s) 81, 139, 378, 528
BstXI CCANNNNNNTGG 3 cut(s) 187, 575, 949
BstYI RGATCY 4 cut(s) 81, 139, 378, 528
BsuI GTATCC 1 cut(s) 1156
BtgI CCRYGG 3 cut(s) 186, 299, 460
BtgZI GCGATG 3 cut(s) 407, 548, 679
BtrI CACGTC 1 cut(s) 1006
BtsCI GGATG 3 cut(s) 196, 385, 747
BtsIMutI CAGTG 5 cut(s) 207, 228, 741, 978, 1186
BtuMI TCGCGA 1 cut(s) 426
Cac8I GCNNGC 7 cut(s) 472, 569, 700, 811, 815, 943, 998
CaiI CAGNNNCTG 1 cut(s) 230
CfoI GCGC 3 cut(s) 404, 830, 1051
Cfr10I RCCGGY 5 cut(s) 698, 707, 716, 809, 1051
Cfr13I GGNCC 7 cut(s) 414, 468, 631, 1033, 1080, 1113, 1138
Cfr42I CCGCGG 1 cut(s) 302
CspCI CAANNNNNGTGG 2 cut(s) 442, 477
CviAII CATG 4 cut(s) 509, 847, 988, 1001
DdeI CTNAG 4 cut(s) 73, 210, 924, 1194
DinI GGCGCC 2 cut(s) 829, 1050
DpnI GATC 6 cut(s) 83, 141, 380, 530, 1073, 1178
DpnII GATC 6 cut(s) 81, 139, 378, 528, 1071, 1176
EaeI YGGCCR 4 cut(s) 163, 577, 811, 939
EciI GGCGGA 1 cut(s) 895
Ecl136II GAGCTC 1 cut(s) 898
Eco24I GRGCYC 2 cut(s) 562, 900
Eco47I GGWCC 4 cut(s) 631, 1033, 1080, 1138
Eco53kI GAGCTC 1 cut(s) 898
Eco57I CTGAAG 1 cut(s) 906
Eco91I GGTNACC 1 cut(s) 551
EcoICRI GAGCTC 1 cut(s) 898
EcoNI CCTNNNNNAGG 1 cut(s) 1130
EcoO109I RGGNCCY 2 cut(s) 468, 631
EcoO65I GGTNACC 1 cut(s) 551
EcoRII CCWGG 2 cut(s) 120, 410
EcoT38I GRGCYC 2 cut(s) 562, 900
EgeI GGCGCC 2 cut(s) 829, 1050
EheI GGCGCC 2 cut(s) 829, 1050
FaeI CATG 4 cut(s) 512, 850, 991, 1004
FaiI YATR 9 cut(s) 66, 510, 773, 848, 989, 1002, 1092, 1110, 1169
FalI AAGNNNNNCTT 4 cut(s) 11, 43, 427, 459
FaqI GGGAC 2 cut(s) 644, 1093
FatI CATG 4 cut(s) 508, 846, 987, 1000
FauI CCCGC 4 cut(s) 306, 368, 458, 1070
Fnu4HI GCNGC 3 cut(s) 441, 784, 1047
FokI GGATG 3 cut(s) 203, 372, 734
FriOI GRGCYC 2 cut(s) 562, 900
Fsp4HI GCNGC 3 cut(s) 441, 784, 1047
FspBI CTAG 2 cut(s) 44, 78
GlaI GCGC 3 cut(s) 403, 829, 1050
GluI GCNGC 3 cut(s) 441, 784, 1047
GsaI CCCAGC 1 cut(s) 420
GsuI CTGGAG 1 cut(s) 821
HaeII RGCGCY 2 cut(s) 831, 1052
HapII CCGG 9 cut(s) 556, 618, 699, 708, 717, 759, 810, 1052, 1083
HhaI GCGC 3 cut(s) 404, 830, 1051
Hin1I GRCGYC 2 cut(s) 828, 1049
Hin1II CATG 4 cut(s) 512, 850, 991, 1004
Hin6I GCGC 3 cut(s) 402, 828, 1049
HinP1I GCGC 3 cut(s) 402, 828, 1049
HincII GTYRAC 1 cut(s) 778
HindII GTYRAC 1 cut(s) 778
HinfI GANTC 1 cut(s) 324
HpaII CCGG 9 cut(s) 556, 618, 699, 708, 717, 759, 810, 1052, 1083
HphI GGTGA 8 cut(s) 267, 286, 319, 563, 697, 715, 853, 1081
Hpy166II GTNNAC 4 cut(s) 778, 978, 1036, 1186
Hpy188I TCNGA 2 cut(s) 917, 1195
Hpy188III TCNNGA 6 cut(s) 109, 224, 425, 593, 838, 1133
Hpy8I GTNNAC 4 cut(s) 778, 978, 1036, 1186
Hpy99I CGWCG 2 cut(s) 588, 875
HpyAV CCTTC 6 cut(s) 37, 512, 599, 808, 881, 1136
HpyCH4III ACNGT 8 cut(s) 118, 187, 538, 575, 745, 865, 982, 1157
HpyCH4IV ACGT 2 cut(s) 583, 1005
HpyCH4V TGCA 4 cut(s) 453, 512, 991, 1174
HpyF10VI GCNNNNNNNGC 8 cut(s) 20, 74, 471, 585, 708, 717, 997, 1057
HpyF3I CTNAG 4 cut(s) 73, 210, 924, 1194
HpySE526I ACGT 2 cut(s) 583, 1005
Hsp92I GRCGYC 2 cut(s) 828, 1049
Hsp92II CATG 4 cut(s) 512, 850, 991, 1004
HspAI GCGC 3 cut(s) 402, 828, 1049
KasI GGCGCC 2 cut(s) 827, 1048
KflI GGGWCCC 1 cut(s) 631
KroI GCCGGC 2 cut(s) 698, 809
KroNI GCCGGC 2 cut(s) 700, 811
KspI CCGCGG 1 cut(s) 302
Kzo9I GATC 6 cut(s) 81, 139, 378, 528, 1071, 1176
LmnI GCTCC 5 cut(s) 292, 822, 860, 895, 908
LweI GCATC 2 cut(s) 223, 281
MaeI CTAG 2 cut(s) 44, 78
MaeII ACGT 2 cut(s) 583, 1005
MaeIII GTNAC 3 cut(s) 551, 802, 859
MalI GATC 6 cut(s) 83, 141, 380, 530, 1073, 1178
MboI GATC 6 cut(s) 81, 139, 378, 528, 1071, 1176
MboII GAAGA 4 cut(s) 351, 497, 605, 1018
MflI RGATCY 4 cut(s) 81, 139, 378, 528
MhlI GDGCHC 3 cut(s) 562, 900, 915
MlsI TGGCCA 3 cut(s) 165, 579, 941
MluCI AATT 1 cut(s) 219
MluNI TGGCCA 3 cut(s) 165, 579, 941
Mly113I GGCGCC 2 cut(s) 828, 1049
MlyI GAGTC 1 cut(s) 318
MmeI TCCRAC 1 cut(s) 277
Mox20I TGGCCA 3 cut(s) 165, 579, 941
MroNI GCCGGC 2 cut(s) 698, 809
MscI TGGCCA 3 cut(s) 165, 579, 941
MslI CAYNNNNRTG 2 cut(s) 185, 1067
Msp20I TGGCCA 3 cut(s) 165, 579, 941
MspA1I CMGCKG 2 cut(s) 301, 625
MspI CCGG 9 cut(s) 556, 618, 699, 708, 717, 759, 810, 1052, 1083
MspR9I CCNGG 5 cut(s) 122, 412, 557, 618, 1084
Mva1269I GAATGC 1 cut(s) 1102
MvaI CCWGG 2 cut(s) 122, 412
MvnI CGCG 3 cut(s) 301, 402, 426
MwoI GCNNNNNNNGC 8 cut(s) 20, 74, 471, 585, 708, 717, 997, 1057
NaeI GCCGGC 2 cut(s) 700, 811
NarI GGCGCC 2 cut(s) 828, 1049
NciI CCSGG 3 cut(s) 557, 618, 1084
NdeII GATC 6 cut(s) 81, 139, 378, 528, 1071, 1176
NgoMIV GCCGGC 2 cut(s) 698, 809
NlaIII CATG 4 cut(s) 512, 850, 991, 1004
NmeAIII GCCGAG 1 cut(s) 879
NmuCI GTSAC 3 cut(s) 551, 802, 859
NruI TCGCGA 1 cut(s) 426
OliI CACNNNNGTG 1 cut(s) 185
PcsI WCGNNNNNNNCGW 2 cut(s) 663, 906
PctI GAATGC 1 cut(s) 1102
PdiI GCCGGC 2 cut(s) 700, 811
PflMI CCANNNNNTGG 2 cut(s) 411, 970
PkrI GCNGC 3 cut(s) 442, 785, 1048
PleI GAGTC 1 cut(s) 318
PluTI GGCGCC 2 cut(s) 831, 1052
PpsI GAGTC 1 cut(s) 318
PpuMI RGGWCCY 1 cut(s) 631
PshAI GACNNNNGTC 1 cut(s) 116
Psp124BI GAGCTC 1 cut(s) 900
Psp5II RGGWCCY 1 cut(s) 631
Psp6I CCWGG 2 cut(s) 120, 410
PspEI GGTNACC 1 cut(s) 551
PspFI CCCAGC 1 cut(s) 416
PspGI CCWGG 2 cut(s) 120, 410
PspPI GGNCC 7 cut(s) 414, 468, 631, 1033, 1080, 1113, 1138
PspPPI RGGWCCY 1 cut(s) 631
PstNI CAGNNNCTG 1 cut(s) 230
PsuI RGATCY 4 cut(s) 81, 139, 378, 528
RruI TCGCGA 1 cut(s) 426
RseI CAYNNNNRTG 2 cut(s) 185, 1067
SacI GAGCTC 1 cut(s) 900
SacII CCGCGG 1 cut(s) 302
SatI GCNGC 3 cut(s) 441, 784, 1047
Sau3AI GATC 6 cut(s) 81, 139, 378, 528, 1071, 1176
Sau96I GGNCC 7 cut(s) 414, 468, 631, 1033, 1080, 1113, 1138
SchI GAGTC 1 cut(s) 318
ScrFI CCNGG 5 cut(s) 122, 412, 557, 618, 1084
SduI GDGCHC 3 cut(s) 562, 900, 915
SfaNI GCATC 2 cut(s) 223, 281
SfoI GGCGCC 2 cut(s) 829, 1050
Sfr303I CCGCGG 1 cut(s) 302
SgrAI CRCCGGYG 1 cut(s) 1051
SgrBI CCGCGG 1 cut(s) 302
SinI GGWCC 4 cut(s) 631, 1033, 1080, 1138
SmiMI CAYNNNNRTG 2 cut(s) 185, 1067
Sse9I AATT 1 cut(s) 219
SspDI GGCGCC 2 cut(s) 827, 1048
SspMI CTAG 2 cut(s) 44, 78
SstI GAGCTC 1 cut(s) 900
StyD4I CCNGG 5 cut(s) 120, 410, 555, 616, 1082
TaaI ACNGT 8 cut(s) 118, 187, 538, 575, 745, 865, 982, 1157
TaiI ACGT 2 cut(s) 586, 1008
TaqI TCGA 3 cut(s) 594, 666, 870
TasI AATT 1 cut(s) 219
TauI GCSGC 3 cut(s) 443, 786, 1049
TscAI CASTG 5 cut(s) 214, 235, 748, 985, 1186
TseFI GTSAC 3 cut(s) 551, 802, 859
Tsp45I GTSAC 3 cut(s) 551, 802, 859
TspDTI ATGAA 3 cut(s) 105, 497, 1118
TspGWI ACGGA 5 cut(s) 141, 530, 847, 969, 1166
TspRI CASTG 5 cut(s) 214, 235, 748, 985, 1186
Van91I CCANNNNNTGG 2 cut(s) 411, 970
VpaK11BI GGWCC 4 cut(s) 631, 1033, 1080, 1138
XagI CCTNNNNNAGG 1 cut(s) 1130
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XspI CTAG 2 cut(s) 44, 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.