MD01G1119900.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016187
Physical Location & Seq
Forward (+)
23359721 .. 23360077
357 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1119900.v1.1.491

Sequence Viewer

Length: 357 bp
ATGTTCGGATCTGTGACTGTGATCGATGACAACCTGACCGAAGGACACGAGTTGGGCTCTGCCGTGCTCGGCAAGGCACAGGGTTTCTACTTAGTTAGTTCGCTGGACGGGAACAGCCACACCATGGCATTTACTGTGTTACTGCACGCAGGTGAACATGATGTGGAGGACACCATTAGCCTCTTTGGGGTCCACCGTACAGCGTCGCCGGTGTCCCACATTGTTGTGATCGGTGGGACTGGGAAGTACGAGAATGCGAACGGGTACACCGCCATTGAGAGCCTGCATCAGGAGGACCAGTACACGACTGATGGGGTGGATACTATTATGCAGATCGGCCTTTACCTTTCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

119

Amino Acids

12.61

Weight (kDa)

4.53

Isoelectric Point (pI)

22.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 1 - 98 2.6e-25 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39430 AT3G55230
fragaria_vesca FvH4_7g21000
malus_domestica MD01G1119900.v1.1 MD07G1189200.v1.1
prunus_persica Prupe.2G226700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365391
rosa_laevigata RLG00000027426
rosa_multiflora Rmu_sc0008527.1_g000004
rosa_roxburghii Rroxscaffold_4G00291260
rosa_rugosa Rorug01G0321600
rosa_samantha Rh1AG330500 Rh1BG292000 Rh1DG323600
rosa_wichuraiana Rw1G029300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 140
Acc36I ACCTGC 1 cut(s) 140
AccB7I CCANNNNNTGG 1 cut(s) 124
AciI CCGC 1 cut(s) 270
AclWI GGATC 1 cut(s) 16
AfaI GTAC 4 cut(s) 199, 248, 266, 302
AfiI CCNNNNNNNGG 3 cut(s) 124, 187, 289
AleI CACNNNNGTG 1 cut(s) 150
Alw21I GWGCWC 1 cut(s) 69
AlwI GGATC 1 cut(s) 16
AoxI GGCC 1 cut(s) 337
AspS9I GGNCC 2 cut(s) 190, 295
AsuHPI GGTGA 1 cut(s) 164
AvaII GGWCC 2 cut(s) 190, 295
BanII GRGCYC 1 cut(s) 59
BauI CACGAG 1 cut(s) 47
Bbv12I GWGCWC 1 cut(s) 69
BccI CCATC 1 cut(s) 305
BceAI ACGGC 1 cut(s) 47
BciVI GTATCC 1 cut(s) 313
BfuAI ACCTGC 1 cut(s) 140
BfuI GTATCC 1 cut(s) 313
Bme18I GGWCC 2 cut(s) 190, 295
BmgT120I GGNCC 2 cut(s) 190, 295
BmiI GGNNCC 1 cut(s) 191
BmrI ACTGGG 1 cut(s) 249
BmsI GCATC 1 cut(s) 295
BmuI ACTGGG 1 cut(s) 249
BplI GAGNNNNNCTC 2 cut(s) 41, 73
Bsa29I ATCGAT 1 cut(s) 24
BsaJI CCNNGG 1 cut(s) 123
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 187, 289
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 2 cut(s) 244, 298
BseCI ATCGAT 1 cut(s) 24
BseDI CCNNGG 1 cut(s) 123
BseLI CCNNNNNNNGG 3 cut(s) 124, 187, 289
BseNI ACTGG 2 cut(s) 244, 298
BsgI GTGCAG 1 cut(s) 128
BshFI GGCC 1 cut(s) 339
BshVI ATCGAT 1 cut(s) 24
BsiHKAI GWGCWC 1 cut(s) 69
BsiSI CCGG 1 cut(s) 209
BslFI GGGAC 2 cut(s) 199, 250
BslI CCNNNNNNNGG 3 cut(s) 124, 187, 289
BsmFI GGGAC 2 cut(s) 199, 250
BsmI GAATGC 1 cut(s) 259
BsnI GGCC 1 cut(s) 339
Bsp1286I GDGCHC 2 cut(s) 59, 69
Bsp143I GATC 4 cut(s) 8, 21, 228, 333
Bsp19I CCATGG 1 cut(s) 123
BspACI CCGC 1 cut(s) 270
BspANI GGCC 1 cut(s) 339
BspDI ATCGAT 1 cut(s) 24
BspLI GGNNCC 1 cut(s) 191
BspMI ACCTGC 1 cut(s) 140
BspPI GGATC 1 cut(s) 16
BsrFI RCCGGY 1 cut(s) 208
BsrI ACTGG 2 cut(s) 244, 298
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 4 cut(s) 8, 21, 228, 333
BssSI CACGAG 1 cut(s) 47
BssT1I CCWWGG 1 cut(s) 123
Bst2BI CACGAG 1 cut(s) 47
Bst4CI ACNGT 3 cut(s) 19, 136, 197
BstC8I GCNNGC 2 cut(s) 147, 284
BstDEI CTNAG 1 cut(s) 91
BstDSI CCRYGG 1 cut(s) 123
BstENI CCTNNNNNAGG 1 cut(s) 287
BstKTI GATC 4 cut(s) 11, 24, 231, 336
BstMBI GATC 4 cut(s) 8, 21, 228, 333
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
Bsu15I ATCGAT 1 cut(s) 24
BsuI GTATCC 1 cut(s) 313
BsuRI GGCC 1 cut(s) 339
BsuTUI ATCGAT 1 cut(s) 24
BtgI CCRYGG 1 cut(s) 123
BveI ACCTGC 1 cut(s) 140
Cac8I GCNNGC 2 cut(s) 147, 284
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 2 cut(s) 190, 295
ClaI ATCGAT 1 cut(s) 24
CseI GACGC 1 cut(s) 192
Csp6I GTAC 4 cut(s) 198, 247, 265, 301
CviAII CATG 2 cut(s) 124, 158
CviJI RGCY 5 cut(s) 57, 117, 180, 282, 339
CviKI_1 RGCY 5 cut(s) 57, 117, 180, 282, 339
CviQI GTAC 4 cut(s) 198, 247, 265, 301
DdeI CTNAG 1 cut(s) 91
DpnI GATC 4 cut(s) 10, 23, 230, 335
DpnII GATC 4 cut(s) 8, 21, 228, 333
Eco130I CCWWGG 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 59
Eco47I GGWCC 2 cut(s) 190, 295
EcoNI CCTNNNNNAGG 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 123
EcoT38I GRGCYC 1 cut(s) 59
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 2 cut(s) 127, 161
FaiI YATR 3 cut(s) 125, 159, 329
FaqI GGGAC 2 cut(s) 199, 250
FatI CATG 2 cut(s) 123, 157
FriOI GRGCYC 1 cut(s) 59
HaeIII GGCC 1 cut(s) 339
HapII CCGG 1 cut(s) 209
HgaI GACGC 1 cut(s) 192
Hin1II CATG 2 cut(s) 127, 161
HpaII CCGG 1 cut(s) 209
HphI GGTGA 1 cut(s) 164
Hpy166II GTNNAC 4 cut(s) 155, 193, 267, 303
Hpy188I TCNGA 2 cut(s) 8, 352
Hpy188III TCNNGA 1 cut(s) 290
Hpy8I GTNNAC 4 cut(s) 155, 193, 267, 303
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 1 cut(s) 35
HpyCH4III ACNGT 3 cut(s) 19, 136, 197
HpyCH4V TGCA 3 cut(s) 145, 286, 331
HpyF3I CTNAG 1 cut(s) 91
Hsp92II CATG 2 cut(s) 127, 161
Kzo9I GATC 4 cut(s) 8, 21, 228, 333
LpnPI CCDG 9 cut(s) 47, 65, 89, 135, 222, 225, 275, 296, 311
LweI GCATC 1 cut(s) 295
MaeIII GTNAC 2 cut(s) 13, 138
MalI GATC 4 cut(s) 10, 23, 230, 335
MboI GATC 4 cut(s) 8, 21, 228, 333
MflI RGATCY 1 cut(s) 8
MhlI GDGCHC 2 cut(s) 59, 69
MnlI CCTC 3 cut(s) 160, 191, 286
MslI CAYNNNNRTG 2 cut(s) 150, 224
MspI CCGG 1 cut(s) 209
Mva1269I GAATGC 1 cut(s) 259
NcoI CCATGG 1 cut(s) 123
NdeII GATC 4 cut(s) 8, 21, 228, 333
NlaIII CATG 2 cut(s) 127, 161
NlaIV GGNNCC 1 cut(s) 191
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 1 cut(s) 13
OliI CACNNNNGTG 1 cut(s) 150
PaqCI CACCTGC 1 cut(s) 140
PctI GAATGC 1 cut(s) 259
PflMI CCANNNNNTGG 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 191
PspPI GGNCC 2 cut(s) 190, 295
PsuI RGATCY 1 cut(s) 8
RsaI GTAC 4 cut(s) 199, 248, 266, 302
RsaNI GTAC 4 cut(s) 198, 247, 265, 301
RseI CAYNNNNRTG 2 cut(s) 150, 224
Sau3AI GATC 4 cut(s) 8, 21, 228, 333
Sau96I GGNCC 2 cut(s) 190, 295
SduI GDGCHC 2 cut(s) 59, 69
SetI ASST 3 cut(s) 36, 154, 348
SfaNI GCATC 1 cut(s) 295
SgrAI CRCCGGYG 1 cut(s) 208
SinI GGWCC 2 cut(s) 190, 295
SmiMI CAYNNNNRTG 2 cut(s) 150, 224
SsiI CCGC 1 cut(s) 270
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 3 cut(s) 19, 136, 197
TaqI TCGA 1 cut(s) 24
TaqII GACCGA 1 cut(s) 53
TatI WGTACW 1 cut(s) 300
TseFI GTSAC 1 cut(s) 13
Tsp45I GTSAC 1 cut(s) 13
Van91I CCANNNNNTGG 1 cut(s) 124
VpaK11BI GGWCC 2 cut(s) 190, 295
XagI CCTNNNNNAGG 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.