AT3G55230

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0016187
Physical Location & Seq
Forward (+)
20471787 .. 20472946
1160 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G55230.1

Sequence Viewer

Length: 921 bp
ATGGCCAAAGCTCTTAGCCTTACAATCTTTCTCTTTCTCTTGATAGCTTCCAATGTCCAATCCGCTCGACTTCTCGATGAGGTTCAAACTCAGCCACAATTAGTCCCCCAGGTACCGGAAGAGGAAGATGATAGTCCACAAGCCGTAACAACAACTCCAACACCAATTCCTCTCCCAGGACCCGCCACGGGAGGACCCGAGCCAATCCTAGAGTTCTTTATGCATGACGTGCTAGGCGGGTCACACCCATCAGCACGTGTGGTTACAGGGATAGTAGCACAAACAGAGGTGAATGGAATACCGTTTTCTAAATCCAGCAACAACATTTTCCCAGTAGATAACGCAGTGCCGCTTGTGAACGCAAACAGCATCAACAACTTAATCAATCCAAACACAGCTCCACTCCTAACAGGACTTAGTGGCTCTCAAGCCAACACCGTAATCCAAAACAGTAACGGTAACTCTCAAGGATCCCTTAGCTCCAACAACCTTCCCTTTGTAACCACGGGTCAGCTCCCTCCCATAGCTGCCCTCCAACAGCTTATGTTTGGCTCTATCACCGTGGTTGACGATGAGCTCACCGAGGGCCATGAGTTGGGCTCTGCGATCATTGGGAGAGCCCAAGGGTTTTATCTCGCTAGCTCTTTGGATGGAACTAGCCAGACCCTTTCGTTGACAGTGTTACTCCACGAAGATCATGATCATCATGACACCCTTGATGATGCCATCAGCTTCTTTGGGGTTCACCGAACCGCTTCTCATGCGTCGCATATCGCTGTTGTGGGTGGAACTGGGAGATTCGAGCATGCTAAAGGGTATGCTGTCGTGGAGACTCTACACAACCAGGAAGACCAACATGTTACTGATGGTCATGATACCATTCTCCATTTCAGTGTTTATCTTACATACTACAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

306

Amino Acids

32.51

Weight (kDa)

4.79

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 174 - 301 4.3e-27 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39430 AT3G55230
fragaria_vesca FvH4_7g21000
malus_domestica MD01G1119900.v1.1 MD07G1189200.v1.1
prunus_persica Prupe.2G226700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365391
rosa_laevigata RLG00000027426
rosa_multiflora Rmu_sc0008527.1_g000004
rosa_roxburghii Rroxscaffold_4G00291260
rosa_rugosa Rorug01G0321600
rosa_samantha Rh1AG330500 Rh1BG292000 Rh1DG323600
rosa_wichuraiana Rw1G029300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 112
AccB1I GGYRCC 1 cut(s) 112
AccB7I CCANNNNNTGG 1 cut(s) 595
AccBSI CCGCTC 1 cut(s) 65
AciI CCGC 5 cut(s) 63, 183, 237, 350, 753
AclWI GGATC 2 cut(s) 465, 478
AcoI YGGCCR 1 cut(s) 3
AcvI CACGTG 1 cut(s) 257
AfaI GTAC 1 cut(s) 114
AfiI CCNNNNNNNGG 4 cut(s) 115, 176, 188, 595
AflIII ACRYGT 2 cut(s) 256, 856
AgsI TTSAA 1 cut(s) 86
AjiI CACGTC 1 cut(s) 229
AjnI CCWGG 3 cut(s) 108, 175, 843
Alw21I GWGCWC 1 cut(s) 579
Alw26I GTCTC 1 cut(s) 824
AlwI GGATC 2 cut(s) 465, 478
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 2 cut(s) 3, 586
ApeKI GCWGC 1 cut(s) 527
Asp700I GAANNNNTTC 1 cut(s) 754
Asp718I GGTACC 1 cut(s) 112
AspS9I GGNCC 3 cut(s) 179, 194, 586
AsuHPI GGTGA 4 cut(s) 301, 550, 571, 737
AsuNHI GCTAGC 1 cut(s) 638
AvaI CYCGRG 1 cut(s) 197
AvaII GGWCC 2 cut(s) 179, 194
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 470
BanI GGYRCC 1 cut(s) 112
BanII GRGCYC 3 cut(s) 579, 602, 622
BbrPI CACGTG 1 cut(s) 257
BbsI GAAGAC 1 cut(s) 855
Bbv12I GWGCWC 1 cut(s) 579
BbvI GCAGC 1 cut(s) 514
BccI CCATC 4 cut(s) 256, 644, 734, 860
BceAI ACGGC 1 cut(s) 128
BciT130I CCWGG 3 cut(s) 110, 177, 845
BclI TGATCA 1 cut(s) 700
BcoDI GTCTC 1 cut(s) 824
BfaI CTAG 4 cut(s) 209, 233, 639, 657
BisI GCNGC 2 cut(s) 350, 528
BlsI GCNGC 2 cut(s) 351, 529
Bme1390I CCNGG 3 cut(s) 110, 177, 845
Bme18I GGWCC 2 cut(s) 179, 194
BmeT110I CYCGRG 1 cut(s) 197
BmgBI CACGTC 1 cut(s) 229
BmgT120I GGNCC 3 cut(s) 179, 194, 586
BmiI GGNNCC 4 cut(s) 114, 181, 196, 472
BmrFI CCNGG 3 cut(s) 110, 177, 845
BmrI ACTGGG 2 cut(s) 326, 801
BmsI GCATC 2 cut(s) 378, 712
BmtI GCTAGC 1 cut(s) 642
BmuI ACTGGG 2 cut(s) 326, 801
BpiI GAAGAC 1 cut(s) 855
BplI GAGNNNNNCTC 2 cut(s) 584, 616
Bpu10I CCTNAGC 1 cut(s) 476
BpuEI CTTGAG 2 cut(s) 411, 450
BsaAI YACGTR 1 cut(s) 257
BsaBI GATNNNNATC 1 cut(s) 699
BsaJI CCNNGG 7 cut(s) 108, 175, 186, 504, 561, 582, 622
BsaWI WCCGGW 1 cut(s) 115
BsaXI ACNNNNNCTCC 2 cut(s) 139, 169
Bsc4I CCNNNNNNNGG 4 cut(s) 115, 176, 188, 595
Bse1I ACTGG 2 cut(s) 332, 796
Bse8I GATNNNNATC 1 cut(s) 699
BseBI CCWGG 3 cut(s) 110, 177, 845
BseDI CCNNGG 7 cut(s) 108, 175, 186, 504, 561, 582, 622
BseGI GGATG 1 cut(s) 655
BseJI GATNNNNATC 1 cut(s) 699
BseLI CCNNNNNNNGG 4 cut(s) 115, 176, 188, 595
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 2 cut(s) 332, 796
BseXI GCAGC 1 cut(s) 514
BshFI GGCC 2 cut(s) 5, 588
BshNI GGYRCC 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 579
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 1 cut(s) 116
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 4 cut(s) 115, 176, 188, 595
BsmAI GTCTC 1 cut(s) 824
BsmFI GGGAC 1 cut(s) 89
BsnI GGCC 2 cut(s) 5, 588
BsoBI CYCGRG 1 cut(s) 197
Bsp1286I GDGCHC 3 cut(s) 579, 602, 622
Bsp143I GATC 4 cut(s) 470, 606, 694, 700
BspACI CCGC 5 cut(s) 63, 183, 237, 350, 753
BspANI GGCC 2 cut(s) 5, 588
BspCNI CTCAG 1 cut(s) 103
BspHI TCATGA 3 cut(s) 697, 706, 871
BspLI GGNNCC 4 cut(s) 114, 181, 196, 472
BspOI GCTAGC 1 cut(s) 642
BspPI GGATC 2 cut(s) 465, 478
BspT107I GGYRCC 1 cut(s) 112
BsrBI CCGCTC 1 cut(s) 65
BsrI ACTGG 2 cut(s) 332, 796
BssECI CCNNGG 7 cut(s) 108, 175, 186, 504, 561, 582, 622
BssMI GATC 4 cut(s) 470, 606, 694, 700
BssT1I CCWWGG 1 cut(s) 622
Bst2UI CCWGG 3 cut(s) 110, 177, 845
Bst4CI ACNGT 6 cut(s) 303, 439, 452, 458, 562, 679
Bst6I CTCTTC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 229
BstBAI YACGTR 1 cut(s) 257
BstC8I GCNNGC 2 cut(s) 640, 807
BstDEI CTNAG 4 cut(s) 14, 90, 416, 476
BstDSI CCRYGG 3 cut(s) 186, 504, 561
BstENI CCTNNNNNAGG 1 cut(s) 174
BstF5I GGATG 1 cut(s) 655
BstKTI GATC 4 cut(s) 473, 609, 697, 703
BstMAI GTCTC 1 cut(s) 824
BstMBI GATC 4 cut(s) 470, 606, 694, 700
BstMWI GCNNNNNNNGC 2 cut(s) 229, 761
BstNI CCWGG 3 cut(s) 110, 177, 845
BstNSI RCATGY 2 cut(s) 809, 860
BstSCI CCNGG 3 cut(s) 108, 175, 843
BstV1I GCAGC 1 cut(s) 514
BstV2I GAAGAC 1 cut(s) 855
BstX2I RGATCY 1 cut(s) 470
BstYI RGATCY 1 cut(s) 470
BsuRI GGCC 2 cut(s) 5, 588
BtgI CCRYGG 3 cut(s) 186, 504, 561
BtrI CACGTC 1 cut(s) 229
BtsCI GGATG 1 cut(s) 655
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 3 cut(s) 351, 684, 898
Cac8I GCNNGC 2 cut(s) 640, 807
CciI TCATGA 3 cut(s) 697, 706, 871
Cfr13I GGNCC 3 cut(s) 179, 194, 586
CseI GACGC 1 cut(s) 753
Csp6I GTAC 1 cut(s) 113
CviAII CATG 8 cut(s) 224, 590, 698, 707, 761, 806, 857, 872
CviQI GTAC 1 cut(s) 113
DdeI CTNAG 4 cut(s) 14, 90, 416, 476
DpnI GATC 4 cut(s) 472, 608, 696, 702
DpnII GATC 4 cut(s) 470, 606, 694, 700
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 114
EarI CTCTTC 1 cut(s) 114
Ecl136II GAGCTC 1 cut(s) 577
Eco130I CCWWGG 1 cut(s) 622
Eco24I GRGCYC 3 cut(s) 579, 602, 622
Eco47I GGWCC 2 cut(s) 179, 194
Eco53kI GAGCTC 1 cut(s) 577
Eco72I CACGTG 1 cut(s) 257
Eco88I CYCGRG 1 cut(s) 197
EcoICRI GAGCTC 1 cut(s) 577
EcoNI CCTNNNNNAGG 1 cut(s) 174
EcoO109I RGGNCCY 2 cut(s) 179, 194
EcoRII CCWGG 3 cut(s) 108, 175, 843
EcoT14I CCWWGG 1 cut(s) 622
EcoT22I ATGCAT 1 cut(s) 225
EcoT38I GRGCYC 3 cut(s) 579, 602, 622
ErhI CCWWGG 1 cut(s) 622
FaeI CATG 8 cut(s) 227, 593, 701, 710, 764, 809, 860, 875
FalI AAGNNNNNCTT 2 cut(s) 459, 491
FaqI GGGAC 1 cut(s) 89
FatI CATG 8 cut(s) 223, 589, 697, 706, 760, 805, 856, 871
FauI CCCGC 2 cut(s) 190, 230
FbaI TGATCA 1 cut(s) 700
Fnu4HI GCNGC 2 cut(s) 350, 528
FokI GGATG 1 cut(s) 662
FriOI GRGCYC 3 cut(s) 579, 602, 622
Fsp4HI GCNGC 2 cut(s) 350, 528
FspBI CTAG 4 cut(s) 209, 233, 639, 657
GluI GCNGC 2 cut(s) 350, 528
HaeIII GGCC 2 cut(s) 5, 588
HapII CCGG 1 cut(s) 116
HgaI GACGC 1 cut(s) 753
Hin1II CATG 8 cut(s) 227, 593, 701, 710, 764, 809, 860, 875
HincII GTYRAC 2 cut(s) 568, 675
HindII GTYRAC 2 cut(s) 568, 675
HindIII AAGCTT 1 cut(s) 915
HinfI GANTC 2 cut(s) 798, 832
HpaII CCGG 1 cut(s) 116
HphI GGTGA 4 cut(s) 301, 550, 571, 737
Hpy166II GTNNAC 5 cut(s) 137, 358, 568, 675, 745
Hpy188III TCNNGA 5 cut(s) 40, 74, 698, 707, 872
Hpy8I GTNNAC 5 cut(s) 137, 358, 568, 675, 745
Hpy99I CGWCG 1 cut(s) 769
HpyAV CCTTC 1 cut(s) 500
HpyCH4III ACNGT 6 cut(s) 303, 439, 452, 458, 562, 679
HpyCH4IV ACGT 2 cut(s) 228, 256
HpyCH4V TGCA 1 cut(s) 223
HpyF10VI GCNNNNNNNGC 2 cut(s) 229, 761
HpyF3I CTNAG 4 cut(s) 14, 90, 416, 476
HpySE526I ACGT 2 cut(s) 228, 256
Hsp92II CATG 8 cut(s) 227, 593, 701, 710, 764, 809, 860, 875
KpnI GGTACC 1 cut(s) 116
Ksp22I TGATCA 1 cut(s) 700
Kzo9I GATC 4 cut(s) 470, 606, 694, 700
LmnI GCTCC 3 cut(s) 403, 485, 519
Lsp1109I GCAGC 1 cut(s) 514
LweI GCATC 2 cut(s) 378, 712
MaeI CTAG 4 cut(s) 209, 233, 639, 657
MaeII ACGT 2 cut(s) 228, 256
MaeIII GTNAC 8 cut(s) 145, 240, 262, 452, 458, 499, 681, 859
MalI GATC 4 cut(s) 472, 608, 696, 702
MbiI CCGCTC 1 cut(s) 65
MboI GATC 4 cut(s) 470, 606, 694, 700
MboII GAAGA 4 cut(s) 131, 137, 704, 860
MflI RGATCY 1 cut(s) 470
MhlI GDGCHC 3 cut(s) 579, 602, 622
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 98, 165
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 826
MmeI TCCRAC 3 cut(s) 182, 507, 559
MnlI CCTC 8 cut(s) 73, 115, 180, 185, 280, 528, 542, 577
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 225
MroXI GAANNNNTTC 1 cut(s) 754
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 380
MslI CAYNNNNRTG 1 cut(s) 891
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 116
MspR9I CCNGG 3 cut(s) 110, 177, 845
MvaI CCWGG 3 cut(s) 110, 177, 845
MwoI GCNNNNNNNGC 2 cut(s) 229, 761
NdeII GATC 4 cut(s) 470, 606, 694, 700
NheI GCTAGC 1 cut(s) 638
NlaIII CATG 8 cut(s) 227, 593, 701, 710, 764, 809, 860, 875
NlaIV GGNNCC 4 cut(s) 114, 181, 196, 472
NmuCI GTSAC 1 cut(s) 240
NsiI ATGCAT 1 cut(s) 225
NspI RCATGY 2 cut(s) 809, 860
PaeI GCATGC 1 cut(s) 809
PagI TCATGA 3 cut(s) 697, 706, 871
PciI ACATGT 1 cut(s) 856
PdmI GAANNNNTTC 1 cut(s) 754
PfeI GAWTC 1 cut(s) 798
PflMI CCANNNNNTGG 1 cut(s) 595
PkrI GCNGC 2 cut(s) 351, 529
PleI GAGTC 1 cut(s) 826
PmaCI CACGTG 1 cut(s) 257
PmlI CACGTG 1 cut(s) 257
PpsI GAGTC 1 cut(s) 826
Ppu21I YACGTR 1 cut(s) 257
PpuMI RGGWCCY 2 cut(s) 179, 194
PscI ACATGT 1 cut(s) 856
Psp124BI GAGCTC 1 cut(s) 579
Psp5II RGGWCCY 2 cut(s) 179, 194
Psp6I CCWGG 3 cut(s) 108, 175, 843
PspCI CACGTG 1 cut(s) 257
PspGI CCWGG 3 cut(s) 108, 175, 843
PspN4I GGNNCC 4 cut(s) 114, 181, 196, 472
PspPI GGNCC 3 cut(s) 179, 194, 586
PspPPI RGGWCCY 2 cut(s) 179, 194
PsuI RGATCY 1 cut(s) 470
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
RseI CAYNNNNRTG 1 cut(s) 891
SacI GAGCTC 1 cut(s) 579
SaqAI TTAA 1 cut(s) 380
SatI GCNGC 2 cut(s) 350, 528
Sau3AI GATC 4 cut(s) 470, 606, 694, 700
Sau96I GGNCC 3 cut(s) 179, 194, 586
SchI GAGTC 1 cut(s) 826
ScrFI CCNGG 3 cut(s) 110, 177, 845
SduI GDGCHC 3 cut(s) 579, 602, 622
SfaNI GCATC 2 cut(s) 378, 712
SinI GGWCC 2 cut(s) 179, 194
SmiMI CAYNNNNRTG 1 cut(s) 891
SmlI CTYRAG 2 cut(s) 426, 465
SmoI CTYRAG 2 cut(s) 426, 465
SphI GCATGC 1 cut(s) 809
Sse9I AATT 2 cut(s) 98, 165
SsiI CCGC 5 cut(s) 63, 183, 237, 350, 753
SspMI CTAG 4 cut(s) 209, 233, 639, 657
SstI GAGCTC 1 cut(s) 579
StyD4I CCNGG 3 cut(s) 108, 175, 843
StyI CCWWGG 1 cut(s) 622
TaaI ACNGT 6 cut(s) 303, 439, 452, 458, 562, 679
TaiI ACGT 2 cut(s) 231, 259
TaqI TCGA 3 cut(s) 67, 75, 801
TasI AATT 2 cut(s) 98, 165
TauI GCSGC 1 cut(s) 352
TfiI GAWTC 1 cut(s) 798
Tru1I TTAA 1 cut(s) 380
Tru9I TTAA 1 cut(s) 380
TscAI CASTG 3 cut(s) 351, 684, 898
TseFI GTSAC 1 cut(s) 240
TseI GCWGC 1 cut(s) 527
Tsp45I GTSAC 1 cut(s) 240
TspRI CASTG 3 cut(s) 351, 684, 898
Van91I CCANNNNNTGG 1 cut(s) 595
VpaK11BI GGWCC 2 cut(s) 179, 194
XagI CCTNNNNNAGG 1 cut(s) 174
XceI RCATGY 2 cut(s) 809, 860
XmnI GAANNNNTTC 1 cut(s) 754
XspI CTAG 4 cut(s) 209, 233, 639, 657
Zsp2I ATGCAT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.