FvH4_7g21000

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0016187
Physical Location & Seq
Forward (+)
16942177 .. 16943704
1528 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g21000.t1

Sequence Viewer

Length: 1239 bp
ATGAAATGTGGTTATTACTCTAAATGCTCTATAGAGTCTAAGCAAATACTCCTCATATCCTCACAACCTAAAATGGCCTCCAAAGCACTCAAGGCCACCCTCTACATTTTGTTCCTAGCCATCATCACACTTGGATATGCCAACTCAGCAAGGATCCTTGATGAGGTAACCAATCCCCTTCCGACAACTGTACTAGCCTCAAACCCGATAAATCCTACCACCCCGTTGCCAAGTGGCCAAATCCCTGTCACCACAACTGTTGATGACAATGTGGACGATGCCCCAGTTGATGATGTCACACCACCCGTTGATGTGGCAACACCAATAGTCCCTCCTGTCACCCCTGCGGTGCCAGAAGCCGAGTCACCCCAACCCGAAGAACCCGTCACCACTACCGTGCCAGCACCTGTAGCGGGTCCCACCATCCCCTCCGCAACCGCACCAATCCCCGTAGCGGGCCCCATTACTCCGACCGCACCTCTCCCGGTGGCAACACCCGTGGCCGGCTCCGCCACCACCGCGGCGAAACCGCATTTGGCCTTCTTCATGCACGACATTCTCGGCGGGTCCCACCCGTCGGTTCGGGTGGTGACGGGGCTCATCGCCAACACCGTGGCCAACGTCCCCTTTTCGAAACCCAACAACAACATCTTCCCCGTCAGCGGTGGGACCCCCCTAAACAACAACAACCTTAACGGCTTTCTCAACAACAACCGCAACAGCCTCCCCTCCTTCGCCGGCCTCACCGGCACCACCGGCCTCACCAACTCCCAAACCAGCACCGTCATCCAAAACTCCGGCAACAACAACATCGTCAACGGCGGCAGCAACCTGCCCTTCGTCACCGCCGGCCAGCTCCCCAACGGCGCCACCCTCCAGAAGCTCATGTTCGGCTCAGTCACCGTCATCGACGACGAACTCACCGAGGGAGAGGAGCTCGGCTCCGCCGTGCTCGGTAAGGCTCAAGGGTTTTACTTGGCAAGCTCTTTGGACGGGAACAGCCACACAATGGCGTTCACTGTTCTCATGCACGGCGAGCATGACGTGGAGGACACCATCAGCTTGTTTGGGGTCCACCGCACTGCGTCGCCGGTGTCACACATTGCGGTGATCGGCGGGACCGGGAAGTATGAGAATGCGAATGGTTACGCGGCCATTGAGAGCCTTCATCAGGAAGACCAGCACACAACGGACGGTGTAGATACCATCATGCAGATCAGTGTTTACCTCTCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

413

Amino Acids

42.67

Weight (kDa)

4.76

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 270 - 409 1.4e-27 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39430 AT3G55230
fragaria_vesca FvH4_7g21000
malus_domestica MD01G1119900.v1.1 MD07G1189200.v1.1
prunus_persica Prupe.2G226700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365391
rosa_laevigata RLG00000027426
rosa_multiflora Rmu_sc0008527.1_g000004
rosa_roxburghii Rroxscaffold_4G00291260
rosa_rugosa Rorug01G0321600
rosa_samantha Rh1AG330500 Rh1BG292000 Rh1DG323600
rosa_wichuraiana Rw1G029300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 840
AccB1I GGYRCC 3 cut(s) 349, 749, 866
AccB7I CCANNNNNTGG 1 cut(s) 1009
AccII CGCG 2 cut(s) 521, 1151
AclWI GGATC 2 cut(s) 148, 161
AcoI YGGCCR 5 cut(s) 235, 501, 615, 850, 1152
AcyI GRCGYC 1 cut(s) 867
AfaI GTAC 1 cut(s) 192
AfiI CCNNNNNNNGG 9 cut(s) 163, 413, 454, 455, 503, 577, 662, 1009, 1171
AjiI CACGTC 1 cut(s) 1045
AjuI GAANNNNNNNTTGG 2 cut(s) 518, 550
AleI CACNNNNGTG 1 cut(s) 395
AluBI AGCT 5 cut(s) 856, 883, 937, 984, 1062
AluI AGCT 5 cut(s) 856, 883, 937, 984, 1062
Alw21I GWGCWC 2 cut(s) 939, 954
AlwI GGATC 2 cut(s) 148, 161
AlwNI CAGNNNCTG 1 cut(s) 407
ApaI GGGCCC 1 cut(s) 461
ApeKI GCWGC 1 cut(s) 825
ArsI GACNNNNNNTTYG 2 cut(s) 369, 401
AspLEI GCGC 1 cut(s) 869
AspS9I GGNCC 7 cut(s) 416, 457, 458, 567, 669, 1072, 1119
AsuC2I CCSGG 2 cut(s) 485, 1123
AsuII TTCGAA 1 cut(s) 632
AvaII GGWCC 5 cut(s) 416, 567, 669, 1072, 1119
BaeGI GKGCMC 1 cut(s) 461
BalI TGGCCA 2 cut(s) 237, 617
BamHI GGATCC 1 cut(s) 153
BanI GGYRCC 3 cut(s) 349, 749, 866
BanII GRGCYC 3 cut(s) 461, 600, 939
BbsI GAAGAC 1 cut(s) 1182
Bbv12I GWGCWC 2 cut(s) 939, 954
BbvI GCAGC 1 cut(s) 837
BccI CCATC 4 cut(s) 128, 431, 1064, 1214
BceAI ACGGC 5 cut(s) 712, 835, 880, 932, 1048
BcnI CCSGG 2 cut(s) 485, 1123
BfaI CTAG 2 cut(s) 116, 194
BfmI CTRYAG 2 cut(s) 30, 408
BfoI RGCGCY 1 cut(s) 870
BfuAI ACCTGC 1 cut(s) 840
BglI GCCNNNNNGGC 1 cut(s) 747
BisI GCNGC 4 cut(s) 522, 823, 826, 1152
BlsI GCNGC 4 cut(s) 523, 824, 827, 1153
Bme1390I CCNGG 2 cut(s) 485, 1123
Bme18I GGWCC 5 cut(s) 416, 567, 669, 1072, 1119
BmgBI CACGTC 1 cut(s) 1045
BmgT120I GGNCC 7 cut(s) 416, 457, 458, 567, 669, 1072, 1119
BmrFI CCNGG 2 cut(s) 485, 1123
BmrI ACTGGG 1 cut(s) 278
BmsI GCATC 1 cut(s) 268
BmuI ACTGGG 1 cut(s) 278
BpiI GAAGAC 1 cut(s) 1182
BplI GAGNNNNNCTC 4 cut(s) 921, 953, 926, 958
BpmI CTGGAG 1 cut(s) 860
Bpu14I TTCGAA 1 cut(s) 632
BpuEI CTTGAG 2 cut(s) 74, 948
BpuMI CCSGG 2 cut(s) 485, 1123
BsaHI GRCGYC 1 cut(s) 867
BsaJI CCNNGG 4 cut(s) 498, 519, 612, 924
Bsc4I CCNNNNNNNGG 9 cut(s) 163, 413, 454, 455, 503, 577, 662, 1009, 1171
Bse118I RCCGGY 6 cut(s) 503, 737, 746, 755, 848, 1090
Bse1I ACTGG 1 cut(s) 284
Bse3DI GCAATG 1 cut(s) 1101
BseDI CCNNGG 4 cut(s) 498, 519, 612, 924
BseGI GGATG 2 cut(s) 423, 786
BseLI CCNNNNNNNGG 9 cut(s) 163, 413, 454, 455, 503, 577, 662, 1009, 1171
BseMI GCAATG 1 cut(s) 1101
BseMII CTCAG 3 cut(s) 159, 909, 1224
BseNI ACTGG 1 cut(s) 284
BseRI GAGGAG 2 cut(s) 41, 947
BseSI GKGCMC 1 cut(s) 461
BseXI GCAGC 1 cut(s) 837
Bsh1236I CGCG 2 cut(s) 521, 1151
Bsh1285I CGRYCG 1 cut(s) 474
BshNI GGYRCC 3 cut(s) 349, 749, 866
BsiEI CGRYCG 1 cut(s) 474
BsiHKAI GWGCWC 2 cut(s) 939, 954
BsiSI CCGG 9 cut(s) 485, 504, 738, 747, 756, 798, 849, 1091, 1122
BslFI GGGAC 6 cut(s) 314, 402, 553, 608, 682, 1132
BslI CCNNNNNNNGG 9 cut(s) 163, 413, 454, 455, 503, 577, 662, 1009, 1171
BsmFI GGGAC 6 cut(s) 314, 402, 553, 608, 682, 1132
BsmI GAATGC 1 cut(s) 1141
Bsp119I TTCGAA 1 cut(s) 632
Bsp120I GGGCCC 1 cut(s) 457
Bsp1286I GDGCHC 4 cut(s) 461, 600, 939, 954
Bsp143I GATC 3 cut(s) 153, 1110, 1215
BspCNI CTCAG 3 cut(s) 158, 908, 1225
BspFNI CGCG 2 cut(s) 521, 1151
BspMI ACCTGC 1 cut(s) 840
BspPI GGATC 2 cut(s) 148, 161
BspT104I TTCGAA 1 cut(s) 632
BspT107I GGYRCC 3 cut(s) 349, 749, 866
BsrDI GCAATG 1 cut(s) 1101
BsrFI RCCGGY 6 cut(s) 503, 737, 746, 755, 848, 1090
BsrI ACTGG 1 cut(s) 284
BssAI RCCGGY 6 cut(s) 503, 737, 746, 755, 848, 1090
BssECI CCNNGG 4 cut(s) 498, 519, 612, 924
BssMI GATC 3 cut(s) 153, 1110, 1215
BssNI GRCGYC 1 cut(s) 867
Bst4CI ACNGT 8 cut(s) 190, 259, 397, 613, 784, 904, 1021, 1196
BstACI GRCGYC 1 cut(s) 867
BstBI TTCGAA 1 cut(s) 632
BstC8I GCNNGC 8 cut(s) 402, 457, 505, 739, 850, 854, 982, 1037
BstDEI CTNAG 4 cut(s) 39, 145, 895, 1233
BstDSI CCRYGG 3 cut(s) 498, 519, 612
BstEII GGTNACC 1 cut(s) 166
BstENI CCTNNNNNAGG 2 cut(s) 161, 1169
BstF5I GGATG 2 cut(s) 423, 786
BstFNI CGCG 2 cut(s) 521, 1151
BstH2I RGCGCY 1 cut(s) 870
BstHHI GCGC 1 cut(s) 869
BstKTI GATC 3 cut(s) 156, 1113, 1218
BstMBI GATC 3 cut(s) 153, 1110, 1215
BstMCI CGRYCG 1 cut(s) 474
BstMWI GCNNNNNNNGC 9 cut(s) 83, 92, 146, 410, 509, 518, 747, 756, 1036
BstPI GGTNACC 1 cut(s) 166
BstSCI CCNGG 2 cut(s) 483, 1121
BstSFI CTRYAG 2 cut(s) 30, 408
BstSLI GKGCMC 1 cut(s) 461
BstUI CGCG 2 cut(s) 521, 1151
BstV1I GCAGC 1 cut(s) 837
BstV2I GAAGAC 1 cut(s) 1182
BstX2I RGATCY 1 cut(s) 153
BstXI CCANNNNNNTGG 1 cut(s) 613
BstYI RGATCY 1 cut(s) 153
BtgI CCRYGG 3 cut(s) 498, 519, 612
BtgZI GCGATG 1 cut(s) 586
BtrI CACGTC 1 cut(s) 1045
BtsCI GGATG 2 cut(s) 423, 786
BtsI GCAGTG 1 cut(s) 1080
BtsIMutI CAGTG 3 cut(s) 1017, 1080, 1225
BveI ACCTGC 1 cut(s) 840
Cac8I GCNNGC 8 cut(s) 402, 457, 505, 739, 850, 854, 982, 1037
CaiI CAGNNNCTG 1 cut(s) 407
CfoI GCGC 1 cut(s) 869
Cfr10I RCCGGY 6 cut(s) 503, 737, 746, 755, 848, 1090
Cfr13I GGNCC 7 cut(s) 416, 457, 458, 567, 669, 1072, 1119
Cfr42I CCGCGG 1 cut(s) 522
CseI GACGC 1 cut(s) 1074
Csp6I GTAC 1 cut(s) 191
CspCI CAANNNNNGTGG 4 cut(s) 241, 276, 480, 515
CviAII CATG 5 cut(s) 547, 886, 1027, 1040, 1210
CviQI GTAC 1 cut(s) 191
DdeI CTNAG 4 cut(s) 39, 145, 895, 1233
DinI GGCGCC 1 cut(s) 868
DpnI GATC 3 cut(s) 155, 1112, 1217
DpnII GATC 3 cut(s) 153, 1110, 1215
EaeI YGGCCR 5 cut(s) 235, 501, 615, 850, 1152
EciI GGCGGA 2 cut(s) 499, 934
Ecl136II GAGCTC 1 cut(s) 937
Eco24I GRGCYC 3 cut(s) 461, 600, 939
Eco47I GGWCC 5 cut(s) 416, 567, 669, 1072, 1119
Eco53kI GAGCTC 1 cut(s) 937
Eco91I GGTNACC 1 cut(s) 166
EcoICRI GAGCTC 1 cut(s) 937
EcoNI CCTNNNNNAGG 2 cut(s) 161, 1169
EcoO109I RGGNCCY 4 cut(s) 416, 458, 567, 669
EcoO65I GGTNACC 1 cut(s) 166
EcoT38I GRGCYC 3 cut(s) 461, 600, 939
EgeI GGCGCC 1 cut(s) 868
EheI GGCGCC 1 cut(s) 868
FaeI CATG 5 cut(s) 550, 889, 1030, 1043, 1213
FaiI YATR 9 cut(s) 32, 56, 138, 548, 887, 1028, 1041, 1131, 1211
FaqI GGGAC 6 cut(s) 314, 402, 553, 608, 682, 1132
FatI CATG 5 cut(s) 546, 885, 1026, 1039, 1209
FauI CCCGC 4 cut(s) 406, 448, 557, 1109
Fnu4HI GCNGC 4 cut(s) 522, 823, 826, 1152
FokI GGATG 2 cut(s) 410, 773
FriOI GRGCYC 3 cut(s) 461, 600, 939
Fsp4HI GCNGC 4 cut(s) 522, 823, 826, 1152
FspBI CTAG 2 cut(s) 116, 194
GlaI GCGC 1 cut(s) 868
GluI GCNGC 4 cut(s) 522, 823, 826, 1152
GsuI CTGGAG 1 cut(s) 860
HaeII RGCGCY 1 cut(s) 870
HapII CCGG 9 cut(s) 485, 504, 738, 747, 756, 798, 849, 1091, 1122
HgaI GACGC 1 cut(s) 1074
HhaI GCGC 1 cut(s) 869
Hin1I GRCGYC 1 cut(s) 867
Hin1II CATG 5 cut(s) 550, 889, 1030, 1043, 1213
Hin6I GCGC 1 cut(s) 867
HinP1I GCGC 1 cut(s) 867
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HinfI GANTC 2 cut(s) 35, 362
HpaII CCGG 9 cut(s) 485, 504, 738, 747, 756, 798, 849, 1091, 1122
Hpy166II GTNNAC 5 cut(s) 274, 817, 1017, 1075, 1225
Hpy188I TCNGA 3 cut(s) 183, 471, 1234
Hpy188III TCNNGA 2 cut(s) 877, 1172
Hpy8I GTNNAC 5 cut(s) 274, 817, 1017, 1075, 1225
Hpy99I CGWCG 4 cut(s) 580, 914, 917, 1090
HpyAV CCTTC 5 cut(s) 188, 550, 742, 847, 1175
HpyCH4III ACNGT 8 cut(s) 190, 259, 397, 613, 784, 904, 1021, 1196
HpyCH4IV ACGT 2 cut(s) 621, 1044
HpyCH4V TGCA 3 cut(s) 550, 1030, 1213
HpyF10VI GCNNNNNNNGC 9 cut(s) 83, 92, 146, 410, 509, 518, 747, 756, 1036
HpyF3I CTNAG 4 cut(s) 39, 145, 895, 1233
HpySE526I ACGT 2 cut(s) 621, 1044
Hsp92I GRCGYC 1 cut(s) 867
Hsp92II CATG 5 cut(s) 550, 889, 1030, 1043, 1213
HspAI GCGC 1 cut(s) 867
KasI GGCGCC 1 cut(s) 866
KflI GGGWCCC 3 cut(s) 416, 567, 669
KroI GCCGGC 3 cut(s) 503, 737, 848
KroNI GCCGGC 3 cut(s) 505, 739, 850
KspI CCGCGG 1 cut(s) 522
Kzo9I GATC 3 cut(s) 153, 1110, 1215
LmnI GCTCC 4 cut(s) 512, 861, 934, 947
Lsp1109I GCAGC 1 cut(s) 837
LweI GCATC 1 cut(s) 268
MaeI CTAG 2 cut(s) 116, 194
MaeII ACGT 2 cut(s) 621, 1044
MalI GATC 3 cut(s) 155, 1112, 1217
MboI GATC 3 cut(s) 153, 1110, 1215
MboII GAAGA 4 cut(s) 389, 535, 643, 1187
MflI RGATCY 1 cut(s) 153
MhlI GDGCHC 4 cut(s) 461, 600, 939, 954
MlsI TGGCCA 2 cut(s) 237, 617
MluNI TGGCCA 2 cut(s) 237, 617
Mly113I GGCGCC 1 cut(s) 867
MlyI GAGTC 2 cut(s) 44, 371
MmeI TCCRAC 2 cut(s) 206, 494
Mox20I TGGCCA 2 cut(s) 237, 617
MroNI GCCGGC 3 cut(s) 503, 737, 848
MscI TGGCCA 2 cut(s) 237, 617
MseI TTAA 1 cut(s) 693
MslI CAYNNNNRTG 2 cut(s) 395, 1106
Msp20I TGGCCA 2 cut(s) 237, 617
MspA1I CMGCKG 2 cut(s) 521, 663
MspI CCGG 9 cut(s) 485, 504, 738, 747, 756, 798, 849, 1091, 1122
MspR9I CCNGG 2 cut(s) 485, 1123
Mva1269I GAATGC 1 cut(s) 1141
MvnI CGCG 2 cut(s) 521, 1151
MwoI GCNNNNNNNGC 9 cut(s) 83, 92, 146, 410, 509, 518, 747, 756, 1036
NaeI GCCGGC 3 cut(s) 505, 739, 850
NarI GGCGCC 1 cut(s) 867
NciI CCSGG 2 cut(s) 485, 1123
NdeII GATC 3 cut(s) 153, 1110, 1215
NgoMIV GCCGGC 3 cut(s) 503, 737, 848
NlaIII CATG 5 cut(s) 550, 889, 1030, 1043, 1213
NmeAIII GCCGAG 3 cut(s) 385, 540, 918
NmuCI GTSAC 9 cut(s) 247, 295, 337, 363, 385, 589, 841, 898, 1095
NspV TTCGAA 1 cut(s) 632
OliI CACNNNNGTG 1 cut(s) 395
PcsI WCGNNNNNNNCGW 3 cut(s) 609, 921, 945
PctI GAATGC 1 cut(s) 1141
PdiI GCCGGC 3 cut(s) 505, 739, 850
PflMI CCANNNNNTGG 1 cut(s) 1009
PkrI GCNGC 4 cut(s) 523, 824, 827, 1153
PleI GAGTC 2 cut(s) 43, 370
PluTI GGCGCC 1 cut(s) 870
PpsI GAGTC 2 cut(s) 43, 370
PpuMI RGGWCCY 3 cut(s) 416, 567, 669
Psp124BI GAGCTC 1 cut(s) 939
Psp5II RGGWCCY 3 cut(s) 416, 567, 669
PspEI GGTNACC 1 cut(s) 166
PspOMI GGGCCC 1 cut(s) 457
PspPI GGNCC 7 cut(s) 416, 457, 458, 567, 669, 1072, 1119
PspPPI RGGWCCY 3 cut(s) 416, 567, 669
PstNI CAGNNNCTG 1 cut(s) 407
PsuI RGATCY 1 cut(s) 153
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
RseI CAYNNNNRTG 2 cut(s) 395, 1106
SacI GAGCTC 1 cut(s) 939
SacII CCGCGG 1 cut(s) 522
SaqAI TTAA 1 cut(s) 693
SatI GCNGC 4 cut(s) 522, 823, 826, 1152
Sau3AI GATC 3 cut(s) 153, 1110, 1215
Sau96I GGNCC 7 cut(s) 416, 457, 458, 567, 669, 1072, 1119
SchI GAGTC 2 cut(s) 44, 371
ScrFI CCNGG 2 cut(s) 485, 1123
SduI GDGCHC 4 cut(s) 461, 600, 939, 954
SfaNI GCATC 1 cut(s) 268
SfcI CTRYAG 2 cut(s) 30, 408
SfoI GGCGCC 1 cut(s) 868
Sfr303I CCGCGG 1 cut(s) 522
SfuI TTCGAA 1 cut(s) 632
SgrAI CRCCGGYG 1 cut(s) 1090
SgrBI CCGCGG 1 cut(s) 522
SinI GGWCC 5 cut(s) 416, 567, 669, 1072, 1119
SmiMI CAYNNNNRTG 2 cut(s) 395, 1106
SmlI CTYRAG 2 cut(s) 89, 963
SmoI CTYRAG 2 cut(s) 89, 963
SspDI GGCGCC 1 cut(s) 866
SspMI CTAG 2 cut(s) 116, 194
SstI GAGCTC 1 cut(s) 939
StyD4I CCNGG 2 cut(s) 483, 1121
TaaI ACNGT 8 cut(s) 190, 259, 397, 613, 784, 904, 1021, 1196
TaiI ACGT 2 cut(s) 624, 1047
TaqI TCGA 2 cut(s) 632, 909
TatI WGTACW 1 cut(s) 190
TauI GCSGC 3 cut(s) 524, 825, 1154
Tru1I TTAA 1 cut(s) 693
Tru9I TTAA 1 cut(s) 693
TscAI CASTG 3 cut(s) 1024, 1087, 1225
TseFI GTSAC 9 cut(s) 247, 295, 337, 363, 385, 589, 841, 898, 1095
TseI GCWGC 1 cut(s) 825
Tsp45I GTSAC 9 cut(s) 247, 295, 337, 363, 385, 589, 841, 898, 1095
TspDTI ATGAA 3 cut(s) 17, 535, 1157
TspGWI ACGGA 1 cut(s) 1205
TspRI CASTG 3 cut(s) 1024, 1087, 1225
Van91I CCANNNNNTGG 1 cut(s) 1009
VpaK11BI GGWCC 5 cut(s) 416, 567, 669, 1072, 1119
XagI CCTNNNNNAGG 2 cut(s) 161, 1169
XspI CTAG 2 cut(s) 116, 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.