RLG00000027426

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0016187
Physical Location & Seq
Reverse (-)
11560290 .. 11561552
1263 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027426

Sequence Viewer

Length: 1263 bp
ATGACCTCCAAAGCAGTCAAGGCCACCTTCTACATTTTATTCCTAGCCATCATTACACTTGGATATGCCAACTCAGCTAGGATCCTTGATGAAGCATCTAATCCCCTCCCGACAACCGTCCAGGCTTCAAACCCGATAAATCCCACCACTCCGTTGCCAAGTGGCCAAATCCCTGCCACCACAACCGTGGATGACAACAAGGATGACGCTGAGGCACCAATTCCAGACACTGATGCCCCTGTTGATGATGTCACACCACCCGTTGATGTGGCATCACCAATAATTGCTCCTGTCACCCCTGCAGTGCCAGAAGCTGATGTCACACCACCCGCTGATGTGGCATCACCAATAGTTGCTCCTGTTACCCCCGAGGTGCCAGAAGCTGACTCACCCCAACCTAAAGAACCCGACACTACTATCGTGCCAACACCCGTAGCGGGCCCCGTCATCCCCTCCGCAATCCCGCCAATCCCGGGCCCAGCCATCCCGACACCAAAAGTGCCGCTCCCAGTTGCAACACCCGTGGCGGGGCCCGCTACCAACGAAGCGAAACCGCATTTGACCTTCTTCATGCACGACATTCTTGGAGGATCCCAACCGTCCGTTCGGGTGGTGACCGGGCTCATCGCCAGCACGGTGGCCAACGTCGCCTTCTCGAAACCCAACAACAACATCTTCCCCGTCAGCGGTGGGACCCCCCTAAACAACAACAACCTCAACGGCTTCCTCGACAACAACCGCAACAACCTCCACTCCATCGCCGGTCTCACCGGCACCACCGGCCTCACCAACTCCCAATCCAGCACTGTCATCCAAAACTCCGGCAACAACAACATAGTCAACGGCGGCGACAACCAGCCCTTCGTCACCGCAGGCCAGCTCCCCAACGGCGCCACCCTCCAGAAGCTCATGTTCGGCTCCGTCACCGTCATCGACGATCAACTGACCGAAGGACAGGAGCTCGGCTCCGCCGTGCTCGGAAAAGCTCAGGGTTTCTACTTGGCCAGCTCTTTGGACGGAAACAGCCACACAATGGCGTTCACTGTTCTCATGCACGGCGAGCATGACGTGGAGGACACCATCAGCTTCTTCGGGGTCCACCGCACTGCGGCGCCGGTGTCGCACATTGCGGTGATCGGCGGGACCGGGAAGTATGAGAATGCGAATGGTTATGGGGCCATTGAGAGCCTTCATCAGGAGGATCAGCACACAACGGACGGTGTGGATACCATAATGCAGATCAGTGTTTACCTCTCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

421

Amino Acids

43.36

Weight (kDa)

4.4

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 278 - 417 2.8e-27 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39430 AT3G55230
fragaria_vesca FvH4_7g21000
malus_domestica MD01G1119900.v1.1 MD07G1189200.v1.1
prunus_persica Prupe.2G226700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365391
rosa_laevigata RLG00000027426
rosa_multiflora Rmu_sc0008527.1_g000004
rosa_roxburghii Rroxscaffold_4G00291260
rosa_rugosa Rorug01G0321600
rosa_samantha Rh1AG330500 Rh1BG292000 Rh1DG323600
rosa_wichuraiana Rw1G029300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 5 cut(s) 214, 373, 773, 890, 1111
AccB7I CCANNNNNTGG 1 cut(s) 1033
AccBSI CCGCTC 1 cut(s) 505
AclWI GGATC 5 cut(s) 76, 89, 585, 598, 1209
AcoI YGGCCR 3 cut(s) 163, 639, 1002
AcyI GRCGYC 2 cut(s) 891, 1112
AfiI CCNNNNNNNGG 8 cut(s) 437, 473, 527, 528, 686, 1033, 1108, 1195
AgsI TTSAA 1 cut(s) 129
AjiI CACGTC 1 cut(s) 1069
AjnI CCWGG 1 cut(s) 120
AjuI GAANNNNNNNTTGG 4 cut(s) 588, 620, 635, 667
AleI CACNNNNGTG 1 cut(s) 185
AluBI AGCT 9 cut(s) 77, 314, 383, 880, 907, 961, 986, 1008, 1086
AluI AGCT 9 cut(s) 77, 314, 383, 880, 907, 961, 986, 1008, 1086
Alw21I GWGCWC 2 cut(s) 963, 978
Alw26I GTCTC 1 cut(s) 770
AlwI GGATC 5 cut(s) 76, 89, 585, 598, 1209
AlwNI CAGNNNCTG 3 cut(s) 230, 314, 383
Ama87I CYCGRG 2 cut(s) 368, 472
ApaI GGGCCC 3 cut(s) 443, 479, 534
AspLEI GCGC 2 cut(s) 893, 1114
AsuC2I CCSGG 4 cut(s) 473, 474, 619, 1147
AvaI CYCGRG 2 cut(s) 368, 472
AvaII GGWCC 3 cut(s) 693, 1096, 1143
BaeGI GKGCMC 3 cut(s) 443, 479, 534
BalI TGGCCA 3 cut(s) 165, 641, 1004
BamHI GGATCC 2 cut(s) 81, 590
BanI GGYRCC 5 cut(s) 214, 373, 773, 890, 1111
BanII GRGCYC 5 cut(s) 443, 479, 534, 624, 963
Bbv12I GWGCWC 2 cut(s) 963, 978
BbvCI CCTCAGC 1 cut(s) 210
BccI CCATC 4 cut(s) 56, 491, 764, 1088
BceAI ACGGC 5 cut(s) 736, 859, 904, 956, 1072
BciT130I CCWGG 1 cut(s) 122
BciVI GTATCC 1 cut(s) 1219
BcnI CCSGG 4 cut(s) 473, 474, 619, 1147
BcoDI GTCTC 1 cut(s) 770
BfaI CTAG 2 cut(s) 44, 78
BfmI CTRYAG 1 cut(s) 300
BfoI RGCGCY 2 cut(s) 894, 1115
BfuI GTATCC 1 cut(s) 1219
BisI GCNGC 3 cut(s) 503, 847, 1110
BlsI GCNGC 3 cut(s) 504, 848, 1111
Bme1390I CCNGG 5 cut(s) 122, 473, 474, 619, 1147
Bme18I GGWCC 3 cut(s) 693, 1096, 1143
BmeT110I CYCGRG 2 cut(s) 368, 472
BmgBI CACGTC 1 cut(s) 1069
BmrFI CCNGG 5 cut(s) 122, 473, 474, 619, 1147
BmrI ACTGGG 1 cut(s) 503
BmsI GCATC 4 cut(s) 104, 223, 281, 350
BmuI ACTGGG 1 cut(s) 503
BoxI GACNNNNGTC 1 cut(s) 116
BplI GAGNNNNNCTC 2 cut(s) 950, 982
BpmI CTGGAG 1 cut(s) 884
Bpu10I CCTNAGC 2 cut(s) 210, 987
BpuMI CCSGG 4 cut(s) 473, 474, 619, 1147
BsaHI GRCGYC 2 cut(s) 891, 1112
BsaI GGTCTC 1 cut(s) 770
BsaJI CCNNGG 4 cut(s) 186, 369, 472, 522
BsaXI ACNNNNNCTCC 2 cut(s) 737, 767
Bsc4I CCNNNNNNNGG 8 cut(s) 437, 473, 527, 528, 686, 1033, 1108, 1195
Bse118I RCCGGY 4 cut(s) 761, 770, 779, 1114
Bse1I ACTGG 1 cut(s) 509
Bse3DI GCAATG 1 cut(s) 1125
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 4 cut(s) 186, 369, 472, 522
BseGI GGATG 5 cut(s) 196, 208, 447, 483, 810
BseLI CCNNNNNNNGG 8 cut(s) 437, 473, 527, 528, 686, 1033, 1108, 1195
BseMI GCAATG 1 cut(s) 1125
BseMII CTCAG 4 cut(s) 87, 201, 1001, 1248
BseNI ACTGG 1 cut(s) 509
BseSI GKGCMC 3 cut(s) 443, 479, 534
BseYI CCCAGC 1 cut(s) 478
BshNI GGYRCC 5 cut(s) 214, 373, 773, 890, 1111
BsiHKAI GWGCWC 2 cut(s) 963, 978
BsiHKCI CYCGRG 2 cut(s) 368, 472
BsiSI CCGG 8 cut(s) 473, 618, 762, 771, 780, 822, 1115, 1146
BslFI GGGAC 2 cut(s) 706, 1156
BslI CCNNNNNNNGG 8 cut(s) 437, 473, 527, 528, 686, 1033, 1108, 1195
BsmAI GTCTC 1 cut(s) 770
BsmFI GGGAC 2 cut(s) 706, 1156
BsmI GAATGC 1 cut(s) 1165
Bso31I GGTCTC 1 cut(s) 770
BsoBI CYCGRG 2 cut(s) 368, 472
Bsp120I GGGCCC 3 cut(s) 439, 475, 530
Bsp1286I GDGCHC 6 cut(s) 443, 479, 534, 624, 963, 978
Bsp143I GATC 6 cut(s) 81, 590, 937, 1134, 1201, 1239
BspCNI CTCAG 4 cut(s) 86, 202, 1000, 1249
BspMAI CTGCAG 1 cut(s) 304
BspPI GGATC 5 cut(s) 76, 89, 585, 598, 1209
BspT107I GGYRCC 5 cut(s) 214, 373, 773, 890, 1111
BspTNI GGTCTC 1 cut(s) 770
BsrBI CCGCTC 1 cut(s) 505
BsrDI GCAATG 1 cut(s) 1125
BsrFI RCCGGY 4 cut(s) 761, 770, 779, 1114
BsrI ACTGG 1 cut(s) 509
BssAI RCCGGY 4 cut(s) 761, 770, 779, 1114
BssECI CCNNGG 4 cut(s) 186, 369, 472, 522
BssMI GATC 6 cut(s) 81, 590, 937, 1134, 1201, 1239
BssNI GRCGYC 2 cut(s) 891, 1112
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 8 cut(s) 118, 187, 600, 637, 808, 928, 1045, 1220
BstACI GRCGYC 2 cut(s) 891, 1112
BstC8I GCNNGC 7 cut(s) 439, 534, 631, 874, 878, 1006, 1061
BstDEI CTNAG 4 cut(s) 73, 210, 987, 1257
BstDSI CCRYGG 2 cut(s) 186, 522
BstEII GGTNACC 1 cut(s) 613
BstENI CCTNNNNNAGG 1 cut(s) 1193
BstF5I GGATG 5 cut(s) 196, 208, 447, 483, 810
BstH2I RGCGCY 2 cut(s) 894, 1115
BstHHI GCGC 2 cut(s) 893, 1114
BstKTI GATC 6 cut(s) 84, 593, 940, 1137, 1204, 1242
BstMAI GTCTC 1 cut(s) 770
BstMBI GATC 6 cut(s) 81, 590, 937, 1134, 1201, 1239
BstMWI GCNNNNNNNGC 8 cut(s) 20, 74, 338, 533, 647, 780, 1060, 1120
BstNI CCWGG 1 cut(s) 122
BstPAI GACNNNNGTC 1 cut(s) 116
BstPI GGTNACC 1 cut(s) 613
BstSCI CCNGG 5 cut(s) 120, 471, 472, 617, 1145
BstSFI CTRYAG 1 cut(s) 300
BstSLI GKGCMC 3 cut(s) 443, 479, 534
BstX2I RGATCY 2 cut(s) 81, 590
BstXI CCANNNNNNTGG 3 cut(s) 187, 637, 1012
BstYI RGATCY 2 cut(s) 81, 590
BsuI GTATCC 1 cut(s) 1219
BtgI CCRYGG 2 cut(s) 186, 522
BtgZI GCGATG 2 cut(s) 610, 742
BtrI CACGTC 1 cut(s) 1069
BtsCI GGATG 5 cut(s) 196, 208, 447, 483, 810
BtsI GCAGTG 2 cut(s) 309, 1104
BtsIMutI CAGTG 6 cut(s) 228, 309, 804, 1041, 1104, 1249
Cac8I GCNNGC 7 cut(s) 439, 534, 631, 874, 878, 1006, 1061
CaiI CAGNNNCTG 3 cut(s) 230, 314, 383
CfoI GCGC 2 cut(s) 893, 1114
Cfr10I RCCGGY 4 cut(s) 761, 770, 779, 1114
Cfr9I CCCGGG 1 cut(s) 472
CseI GACGC 1 cut(s) 215
CspCI CAANNNNNGTGG 2 cut(s) 504, 539
CviAII CATG 4 cut(s) 571, 910, 1051, 1064
DdeI CTNAG 4 cut(s) 73, 210, 987, 1257
DinI GGCGCC 2 cut(s) 892, 1113
DpnI GATC 6 cut(s) 83, 592, 939, 1136, 1203, 1241
DpnII GATC 6 cut(s) 81, 590, 937, 1134, 1201, 1239
EaeI YGGCCR 3 cut(s) 163, 639, 1002
EciI GGCGGA 1 cut(s) 958
Ecl136II GAGCTC 1 cut(s) 961
Eco24I GRGCYC 5 cut(s) 443, 479, 534, 624, 963
Eco31I GGTCTC 1 cut(s) 770
Eco47I GGWCC 3 cut(s) 693, 1096, 1143
Eco53kI GAGCTC 1 cut(s) 961
Eco88I CYCGRG 2 cut(s) 368, 472
Eco91I GGTNACC 1 cut(s) 613
EcoICRI GAGCTC 1 cut(s) 961
EcoNI CCTNNNNNAGG 1 cut(s) 1193
EcoO109I RGGNCCY 3 cut(s) 440, 530, 693
EcoO65I GGTNACC 1 cut(s) 613
EcoRII CCWGG 1 cut(s) 120
EcoT38I GRGCYC 5 cut(s) 443, 479, 534, 624, 963
EgeI GGCGCC 2 cut(s) 892, 1113
EheI GGCGCC 2 cut(s) 892, 1113
FaeI CATG 4 cut(s) 574, 913, 1054, 1067
FaiI YATR 9 cut(s) 66, 572, 836, 911, 1052, 1065, 1155, 1173, 1232
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 2 cut(s) 706, 1156
FatI CATG 4 cut(s) 570, 909, 1050, 1063
FauI CCCGC 6 cut(s) 337, 430, 471, 520, 541, 1133
Fnu4HI GCNGC 3 cut(s) 503, 847, 1110
FokI GGATG 5 cut(s) 203, 215, 434, 470, 797
FriOI GRGCYC 5 cut(s) 443, 479, 534, 624, 963
Fsp4HI GCNGC 3 cut(s) 503, 847, 1110
FspBI CTAG 2 cut(s) 44, 78
GlaI GCGC 2 cut(s) 892, 1113
GluI GCNGC 3 cut(s) 503, 847, 1110
GsaI CCCAGC 1 cut(s) 482
GsuI CTGGAG 1 cut(s) 884
HaeII RGCGCY 2 cut(s) 894, 1115
HapII CCGG 8 cut(s) 473, 618, 762, 771, 780, 822, 1115, 1146
HgaI GACGC 1 cut(s) 215
HhaI GCGC 2 cut(s) 893, 1114
Hin1I GRCGYC 2 cut(s) 891, 1112
Hin1II CATG 4 cut(s) 574, 913, 1054, 1067
Hin6I GCGC 2 cut(s) 891, 1112
HinP1I GCGC 2 cut(s) 891, 1112
HincII GTYRAC 1 cut(s) 841
HindII GTYRAC 1 cut(s) 841
HinfI GANTC 1 cut(s) 386
HpaII CCGG 8 cut(s) 473, 618, 762, 771, 780, 822, 1115, 1146
Hpy166II GTNNAC 4 cut(s) 841, 1041, 1099, 1249
Hpy188I TCNGA 2 cut(s) 980, 1258
Hpy188III TCNNGA 6 cut(s) 109, 224, 487, 655, 901, 1196
Hpy8I GTNNAC 4 cut(s) 841, 1041, 1099, 1249
Hpy99I CGWCG 2 cut(s) 650, 938
HpyAV CCTTC 6 cut(s) 37, 574, 661, 871, 944, 1199
HpyCH4III ACNGT 8 cut(s) 118, 187, 600, 637, 808, 928, 1045, 1220
HpyCH4IV ACGT 2 cut(s) 645, 1068
HpyCH4V TGCA 5 cut(s) 302, 515, 574, 1054, 1237
HpyF10VI GCNNNNNNNGC 8 cut(s) 20, 74, 338, 533, 647, 780, 1060, 1120
HpyF3I CTNAG 4 cut(s) 73, 210, 987, 1257
HpySE526I ACGT 2 cut(s) 645, 1068
Hsp92I GRCGYC 2 cut(s) 891, 1112
Hsp92II CATG 4 cut(s) 574, 913, 1054, 1067
HspAI GCGC 2 cut(s) 891, 1112
KasI GGCGCC 2 cut(s) 890, 1111
KflI GGGWCCC 1 cut(s) 693
Kzo9I GATC 6 cut(s) 81, 590, 937, 1134, 1201, 1239
LmnI GCTCC 7 cut(s) 292, 361, 510, 885, 923, 958, 971
LweI GCATC 4 cut(s) 104, 223, 281, 350
MaeI CTAG 2 cut(s) 44, 78
MaeII ACGT 2 cut(s) 645, 1068
MaeIII GTNAC 7 cut(s) 250, 292, 319, 361, 613, 865, 922
MalI GATC 6 cut(s) 83, 592, 939, 1136, 1203, 1241
MbiI CCGCTC 1 cut(s) 505
MboI GATC 6 cut(s) 81, 590, 937, 1134, 1201, 1239
MboII GAAGA 3 cut(s) 559, 667, 1081
MflI RGATCY 2 cut(s) 81, 590
MhlI GDGCHC 6 cut(s) 443, 479, 534, 624, 963, 978
MlsI TGGCCA 3 cut(s) 165, 641, 1004
MluCI AATT 2 cut(s) 219, 282
MluNI TGGCCA 3 cut(s) 165, 641, 1004
Mly113I GGCGCC 2 cut(s) 891, 1112
MlyI GAGTC 1 cut(s) 380
Mox20I TGGCCA 3 cut(s) 165, 641, 1004
MscI TGGCCA 3 cut(s) 165, 641, 1004
MslI CAYNNNNRTG 2 cut(s) 185, 1130
Msp20I TGGCCA 3 cut(s) 165, 641, 1004
MspA1I CMGCKG 2 cut(s) 332, 687
MspI CCGG 8 cut(s) 473, 618, 762, 771, 780, 822, 1115, 1146
MspR9I CCNGG 5 cut(s) 122, 473, 474, 619, 1147
Mva1269I GAATGC 1 cut(s) 1165
MvaI CCWGG 1 cut(s) 122
MwoI GCNNNNNNNGC 8 cut(s) 20, 74, 338, 533, 647, 780, 1060, 1120
NarI GGCGCC 2 cut(s) 891, 1112
NciI CCSGG 4 cut(s) 473, 474, 619, 1147
NdeII GATC 6 cut(s) 81, 590, 937, 1134, 1201, 1239
NlaIII CATG 4 cut(s) 574, 913, 1054, 1067
NmeAIII GCCGAG 1 cut(s) 942
NmuCI GTSAC 6 cut(s) 250, 292, 319, 613, 865, 922
OliI CACNNNNGTG 1 cut(s) 185
PcsI WCGNNNNNNNCGW 2 cut(s) 726, 969
PctI GAATGC 1 cut(s) 1165
PflMI CCANNNNNTGG 1 cut(s) 1033
PkrI GCNGC 3 cut(s) 504, 848, 1111
PleI GAGTC 1 cut(s) 380
PluTI GGCGCC 2 cut(s) 894, 1115
PpsI GAGTC 1 cut(s) 380
PpuMI RGGWCCY 1 cut(s) 693
PshAI GACNNNNGTC 1 cut(s) 116
Psp124BI GAGCTC 1 cut(s) 963
Psp5II RGGWCCY 1 cut(s) 693
Psp6I CCWGG 1 cut(s) 120
PspEI GGTNACC 1 cut(s) 613
PspFI CCCAGC 1 cut(s) 478
PspGI CCWGG 1 cut(s) 120
PspOMI GGGCCC 3 cut(s) 439, 475, 530
PspPPI RGGWCCY 1 cut(s) 693
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 3 cut(s) 230, 314, 383
PsuI RGATCY 2 cut(s) 81, 590
RseI CAYNNNNRTG 2 cut(s) 185, 1130
SacI GAGCTC 1 cut(s) 963
SatI GCNGC 3 cut(s) 503, 847, 1110
Sau3AI GATC 6 cut(s) 81, 590, 937, 1134, 1201, 1239
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 5 cut(s) 122, 473, 474, 619, 1147
SduI GDGCHC 6 cut(s) 443, 479, 534, 624, 963, 978
SfaNI GCATC 4 cut(s) 104, 223, 281, 350
SfcI CTRYAG 1 cut(s) 300
SfoI GGCGCC 2 cut(s) 892, 1113
SgrAI CRCCGGYG 1 cut(s) 1114
SinI GGWCC 3 cut(s) 693, 1096, 1143
SmaI CCCGGG 1 cut(s) 474
SmiMI CAYNNNNRTG 2 cut(s) 185, 1130
Sse9I AATT 2 cut(s) 219, 282
SspDI GGCGCC 2 cut(s) 890, 1111
SspMI CTAG 2 cut(s) 44, 78
SstI GAGCTC 1 cut(s) 963
StyD4I CCNGG 5 cut(s) 120, 471, 472, 617, 1145
TaaI ACNGT 8 cut(s) 118, 187, 600, 637, 808, 928, 1045, 1220
TaiI ACGT 2 cut(s) 648, 1071
TaqI TCGA 3 cut(s) 656, 729, 933
TaqII GACCGA 1 cut(s) 962
TasI AATT 2 cut(s) 219, 282
TauI GCSGC 3 cut(s) 505, 849, 1112
TscAI CASTG 6 cut(s) 235, 309, 811, 1048, 1111, 1249
TseFI GTSAC 6 cut(s) 250, 292, 319, 613, 865, 922
Tsp45I GTSAC 6 cut(s) 250, 292, 319, 613, 865, 922
TspDTI ATGAA 3 cut(s) 105, 559, 1181
TspGWI ACGGA 5 cut(s) 141, 592, 910, 1032, 1229
TspMI CCCGGG 1 cut(s) 472
TspRI CASTG 6 cut(s) 235, 309, 811, 1048, 1111, 1249
Van91I CCANNNNNTGG 1 cut(s) 1033
VpaK11BI GGWCC 3 cut(s) 693, 1096, 1143
XagI CCTNNNNNAGG 1 cut(s) 1193
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XmaI CCCGGG 1 cut(s) 472
XspI CTAG 2 cut(s) 44, 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.