AT2G40316

Autophagy-related protein 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0010981
Physical Location & Seq
Forward (+)
16836104 .. 16839446
3343 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G40316.3

Sequence Viewer

Length: 831 bp
ATGTCGGGGAAACTTTGGATCCCGAGATTTATTCTCATCACTCTACTGTTTATTAGCTCACGAGTTAACTCTCAATGTGAATTCAGTTTCACTGGACGCAACAAGGTCTTTCATTTCAACCTAGCATCTTCGATTCGGAATTATCCTCACGGTGTGCTCAGCGAAGATGGGTTTTATAAAGTGGAAGCGAACAGTTCCGTGTTATGGTTTCAGCTTTGTGACGTGCTGATTTTCAATCATGATCCCCCTCGATGTGTTGGTTGCGAAGATTGTGGAGGTCCATCTCACTGCGGAACGTCATGTAGTGCACTTGTGTCAGAAAATGTAAGAGGGTATGATGTATGCACTTCTCTAGGGCATGCCTCAAGCACAAAAGTTGATATTATTGATAAAGAGGATCCTGGCAAAGGCGTCATTGTTCAGATGTCAACTGGAAATAGGAACCAAAATTGCTCGCTTTCAGTTTCTGTTATTTGCCAGAAAACTAAAGTTGATGGACCACTCACTTTGGCGAAATCTGGTACCTGTCAGTATGCTACTCAGCTGCGACATCCTGCCGGTTGTGCAGCAGCTATATCCGGCCATAGTAGTGGATGGGGCTGGTTTAGTACCTTACTAATCATTATCTTGTGCCTGTTTGGAGCTTATCTGGTGGGTGGTGCAGTATATCGGTATTTCTCCCTTGGAATTCGTGGCATAGATGTAATCCCGAATATGGATTACTGGGCCACTGTACCTCACAGTATACAGAGCTGTTTTGGCTCACTATTTTCAAGATTCGGGGGGTCTATTCGTGGTCAGCAAACCTCATATTCTCAGGTCAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
ATG27

Protein Analysis

276

Amino Acids

30.04

Weight (kDa)

8.37

Isoelectric Point (pI)

29.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG27 PF09451 31 - 201 8.3e-18 Autophagy-related protein 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010981)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40316 AT2G40316 AT2G40316
fragaria_vesca FvH4_7g25140 FvH4_7g25140
malus_domestica MD01G1158200.v1.1 MD07G1226900.v1.1
prunus_persica Prupe.2G256500_v2.0.a1
pyrus_communis pycom01g17490 pycom07g20550
rosa_chinensis RchiOBHm_Chr1g0371041
rosa_laevigata RLG00000026990
rosa_multiflora Rmu_sc0001018.1_g000028
rosa_roxburghii Rroxscaffold_4G00286040
rosa_rugosa Rorug01G0361000 Rorug01G0361100
rosa_samantha Rh1AG371300 Rh1CG348100 Rh1DG366000
rosa_wichuraiana Rw1G032660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
Acc65I GGTACC 1 cut(s) 521
AccB1I GGYRCC 1 cut(s) 521
AccI GTMKAC 1 cut(s) 745
AciI CCGC 1 cut(s) 291
AclWI GGATC 5 cut(s) 13, 26, 236, 392, 405
AcoI YGGCCR 1 cut(s) 580
AcsI RAATTY 2 cut(s) 80, 687
AcyI GRCGYC 1 cut(s) 411
AdeI CACNNNGTG 1 cut(s) 154
AfaI GTAC 3 cut(s) 523, 610, 735
AfiI CCNNNNNNNGG 3 cut(s) 204, 407, 715
AgsI TTSAA 3 cut(s) 118, 235, 774
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 1 cut(s) 400
AluBI AGCT 6 cut(s) 57, 214, 544, 572, 644, 753
AluI AGCT 6 cut(s) 57, 214, 544, 572, 644, 753
Alw21I GWGCWC 2 cut(s) 159, 310
Alw44I GTGCAC 1 cut(s) 306
AlwI GGATC 5 cut(s) 13, 26, 236, 392, 405
AlwNI CAGNNNCTG 1 cut(s) 467
Ama87I CYCGRG 1 cut(s) 22
AoxI GGCC 2 cut(s) 580, 726
ApaLI GTGCAC 1 cut(s) 306
ApeKI GCWGC 3 cut(s) 544, 566, 569
ApoI RAATTY 2 cut(s) 80, 687
Asp718I GGTACC 1 cut(s) 521
AspS9I GGNCC 3 cut(s) 278, 497, 726
AvaI CYCGRG 1 cut(s) 22
AvaII GGWCC 2 cut(s) 278, 497
BaeGI GKGCMC 1 cut(s) 310
BamHI GGATCC 2 cut(s) 18, 397
BanI GGYRCC 1 cut(s) 521
BauI CACGAG 1 cut(s) 60
Bbv12I GWGCWC 2 cut(s) 159, 310
BbvI GCAGC 3 cut(s) 531, 578, 581
BccI CCATC 4 cut(s) 161, 289, 488, 588
BciT130I CCWGG 1 cut(s) 402
BfaI CTAG 2 cut(s) 122, 353
BisI GCNGC 3 cut(s) 545, 567, 570
BlpI GCTNAGC 1 cut(s) 158
BlsI GCNGC 3 cut(s) 546, 568, 571
Bme1390I CCNGG 1 cut(s) 402
Bme18I GGWCC 2 cut(s) 278, 497
BmeT110I CYCGRG 1 cut(s) 22
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 3 cut(s) 278, 497, 726
BmiI GGNNCC 4 cut(s) 20, 399, 443, 523
BmrFI CCNGG 1 cut(s) 402
BmrI ACTGGG 1 cut(s) 733
BmsI GCATC 1 cut(s) 134
BmuI ACTGGG 1 cut(s) 733
Bpu1102I GCTNAGC 1 cut(s) 158
BpuEI CTTGAG 1 cut(s) 349
BsaHI GRCGYC 1 cut(s) 411
BsaJI CCNNGG 1 cut(s) 682
Bsc4I CCNNNNNNNGG 3 cut(s) 204, 407, 715
Bse118I RCCGGY 1 cut(s) 557
Bse1I ACTGG 3 cut(s) 97, 436, 728
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 1 cut(s) 682
BseGI GGATG 2 cut(s) 550, 599
BseLI CCNNNNNNNGG 3 cut(s) 204, 407, 715
BseMII CTCAG 3 cut(s) 172, 554, 830
BseNI ACTGG 3 cut(s) 97, 436, 728
BseSI GKGCMC 1 cut(s) 310
BseXI GCAGC 3 cut(s) 531, 578, 581
BsgI GTGCAG 2 cut(s) 585, 681
BshFI GGCC 2 cut(s) 582, 728
BshNI GGYRCC 1 cut(s) 521
BsiHKAI GWGCWC 2 cut(s) 159, 310
BsiHKCI CYCGRG 1 cut(s) 22
BsiSI CCGG 2 cut(s) 558, 579
BslI CCNNNNNNNGG 3 cut(s) 204, 407, 715
BsnI GGCC 2 cut(s) 582, 728
BsoBI CYCGRG 1 cut(s) 22
Bsp1286I GDGCHC 2 cut(s) 159, 310
Bsp143I GATC 3 cut(s) 18, 241, 397
Bsp1720I GCTNAGC 1 cut(s) 158
BspACI CCGC 1 cut(s) 291
BspANI GGCC 2 cut(s) 582, 728
BspCNI CTCAG 3 cut(s) 171, 553, 829
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 4 cut(s) 20, 399, 443, 523
BspPI GGATC 5 cut(s) 13, 26, 236, 392, 405
BspT107I GGYRCC 1 cut(s) 521
BsrFI RCCGGY 1 cut(s) 557
BsrI ACTGG 3 cut(s) 97, 436, 728
BssAI RCCGGY 1 cut(s) 557
BssECI CCNNGG 1 cut(s) 682
BssMI GATC 3 cut(s) 18, 241, 397
BssNAI GTATAC 1 cut(s) 746
BssNI GRCGYC 1 cut(s) 411
BssSI CACGAG 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 682
Bst1107I GTATAC 1 cut(s) 746
Bst2BI CACGAG 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 5 cut(s) 48, 152, 194, 733, 743
BstACI GRCGYC 1 cut(s) 411
BstC8I GCNNGC 2 cut(s) 360, 455
BstDEI CTNAG 3 cut(s) 158, 540, 816
BstENI CCTNNNNNAGG 1 cut(s) 405
BstF5I GGATG 2 cut(s) 550, 599
BstKTI GATC 3 cut(s) 21, 244, 400
BstMBI GATC 3 cut(s) 18, 241, 397
BstMWI GCNNNNNNNGC 2 cut(s) 563, 759
BstNI CCWGG 1 cut(s) 402
BstNSI RCATGY 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 400
BstSLI GKGCMC 1 cut(s) 310
BstV1I GCAGC 3 cut(s) 531, 578, 581
BstX2I RGATCY 2 cut(s) 18, 397
BstXI CCANNNNNNTGG 1 cut(s) 590
BstYI RGATCY 2 cut(s) 18, 397
BstZ17I GTATAC 1 cut(s) 746
BsuRI GGCC 2 cut(s) 582, 728
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 2 cut(s) 550, 599
BtsI GCAGTG 1 cut(s) 286
BtsIMutI CAGTG 3 cut(s) 90, 286, 729
Cac8I GCNNGC 2 cut(s) 360, 455
CaiI CAGNNNCTG 1 cut(s) 467
CciI TCATGA 1 cut(s) 238
Cfr10I RCCGGY 1 cut(s) 557
Cfr13I GGNCC 3 cut(s) 278, 497, 726
CseI GACGC 2 cut(s) 105, 400
Csp6I GTAC 3 cut(s) 522, 609, 734
CspCI CAANNNNNGTGG 2 cut(s) 489, 524
CviAII CATG 3 cut(s) 239, 300, 359
CviQI GTAC 3 cut(s) 522, 609, 734
DdeI CTNAG 3 cut(s) 158, 540, 816
DpnI GATC 3 cut(s) 20, 243, 399
DpnII GATC 3 cut(s) 18, 241, 397
DraIII CACNNNGTG 1 cut(s) 154
EaeI YGGCCR 1 cut(s) 580
Eco130I CCWWGG 1 cut(s) 682
Eco47I GGWCC 2 cut(s) 278, 497
Eco88I CYCGRG 1 cut(s) 22
EcoNI CCTNNNNNAGG 1 cut(s) 405
EcoRI GAATTC 2 cut(s) 80, 687
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 682
ErhI CCWWGG 1 cut(s) 682
FaeI CATG 3 cut(s) 242, 303, 362
FatI CATG 3 cut(s) 238, 299, 358
FblI GTMKAC 1 cut(s) 745
Fnu4HI GCNGC 3 cut(s) 545, 567, 570
FokI GGATG 2 cut(s) 537, 606
Fsp4HI GCNGC 3 cut(s) 545, 567, 570
FspBI CTAG 2 cut(s) 122, 353
GluI GCNGC 3 cut(s) 545, 567, 570
HaeIII GGCC 2 cut(s) 582, 728
HapII CCGG 2 cut(s) 558, 579
HgaI GACGC 2 cut(s) 105, 400
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 3 cut(s) 242, 303, 362
HincII GTYRAC 3 cut(s) 67, 429, 823
HindII GTYRAC 3 cut(s) 67, 429, 823
HinfI GANTC 2 cut(s) 133, 777
HpaI GTTAAC 1 cut(s) 67
HpaII CCGG 2 cut(s) 558, 579
Hpy166II GTNNAC 5 cut(s) 67, 308, 429, 746, 823
Hpy188I TCNGA 4 cut(s) 138, 319, 423, 830
Hpy188III TCNNGA 5 cut(s) 22, 60, 239, 709, 774
Hpy8I GTNNAC 5 cut(s) 67, 308, 429, 746, 823
HpyCH4III ACNGT 5 cut(s) 48, 152, 194, 733, 743
HpyCH4IV ACGT 2 cut(s) 222, 296
HpyCH4V TGCA 4 cut(s) 308, 345, 566, 662
HpyF10VI GCNNNNNNNGC 2 cut(s) 563, 759
HpyF3I CTNAG 3 cut(s) 158, 540, 816
HpySE526I ACGT 2 cut(s) 222, 296
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 3 cut(s) 242, 303, 362
KpnI GGTACC 1 cut(s) 525
KspAI GTTAAC 1 cut(s) 67
Kzo9I GATC 3 cut(s) 18, 241, 397
LmnI GCTCC 1 cut(s) 641
Lsp1109I GCAGC 3 cut(s) 531, 578, 581
LweI GCATC 1 cut(s) 134
MaeI CTAG 2 cut(s) 122, 353
MaeII ACGT 2 cut(s) 222, 296
MaeIII GTNAC 1 cut(s) 218
MalI GATC 3 cut(s) 20, 243, 399
MboI GATC 3 cut(s) 18, 241, 397
MboII GAAGA 3 cut(s) 120, 176, 278
MflI RGATCY 2 cut(s) 18, 397
MhlI GDGCHC 2 cut(s) 159, 310
MluCI AATT 4 cut(s) 80, 139, 448, 687
MnlI CCTC 8 cut(s) 156, 258, 269, 323, 373, 388, 747, 817
MseI TTAA 1 cut(s) 66
MslI CAYNNNNRTG 1 cut(s) 588
MspA1I CMGCKG 1 cut(s) 544
MspI CCGG 2 cut(s) 558, 579
MspR9I CCNGG 1 cut(s) 402
MvaI CCWGG 1 cut(s) 402
MwoI GCNNNNNNNGC 2 cut(s) 563, 759
NdeII GATC 3 cut(s) 18, 241, 397
NlaIII CATG 3 cut(s) 242, 303, 362
NlaIV GGNNCC 4 cut(s) 20, 399, 443, 523
NmuCI GTSAC 1 cut(s) 218
NspI RCATGY 1 cut(s) 362
PaeI GCATGC 1 cut(s) 362
PagI TCATGA 1 cut(s) 238
PfeI GAWTC 2 cut(s) 133, 777
PkrI GCNGC 3 cut(s) 546, 568, 571
PsiI TTATAA 1 cut(s) 177
Psp6I CCWGG 1 cut(s) 400
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 4 cut(s) 20, 399, 443, 523
PspPI GGNCC 3 cut(s) 278, 497, 726
PstNI CAGNNNCTG 1 cut(s) 467
PsuI RGATCY 2 cut(s) 18, 397
PvuII CAGCTG 1 cut(s) 544
RsaI GTAC 3 cut(s) 523, 610, 735
RsaNI GTAC 3 cut(s) 522, 609, 734
RseI CAYNNNNRTG 1 cut(s) 588
SaqAI TTAA 1 cut(s) 66
SatI GCNGC 3 cut(s) 545, 567, 570
Sau3AI GATC 3 cut(s) 18, 241, 397
Sau96I GGNCC 3 cut(s) 278, 497, 726
ScrFI CCNGG 1 cut(s) 402
SduI GDGCHC 2 cut(s) 159, 310
SfaNI GCATC 1 cut(s) 134
SinI GGWCC 2 cut(s) 278, 497
SmiMI CAYNNNNRTG 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 364
SmoI CTYRAG 1 cut(s) 364
SphI GCATGC 1 cut(s) 362
Sse9I AATT 4 cut(s) 80, 139, 448, 687
SsiI CCGC 1 cut(s) 291
SspMI CTAG 2 cut(s) 122, 353
StyD4I CCNGG 1 cut(s) 400
StyI CCWWGG 1 cut(s) 682
TaaI ACNGT 5 cut(s) 48, 152, 194, 733, 743
TaiI ACGT 2 cut(s) 225, 299
TaqI TCGA 2 cut(s) 131, 250
TasI AATT 4 cut(s) 80, 139, 448, 687
TfiI GAWTC 2 cut(s) 133, 777
Tru1I TTAA 1 cut(s) 66
Tru9I TTAA 1 cut(s) 66
TscAI CASTG 3 cut(s) 97, 293, 736
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 3 cut(s) 544, 566, 569
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 1 cut(s) 101
TspGWI ACGGA 1 cut(s) 187
TspRI CASTG 3 cut(s) 97, 293, 736
VneI GTGCAC 1 cut(s) 306
VpaK11BI GGWCC 2 cut(s) 278, 497
XagI CCTNNNNNAGG 1 cut(s) 405
XapI RAATTY 2 cut(s) 80, 687
XceI RCATGY 1 cut(s) 362
XmiI GTMKAC 1 cut(s) 745
XspI CTAG 2 cut(s) 122, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.