Rorug01G0361000

Autophagy-related protein 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0010981
Physical Location & Seq
Reverse (-)
47657674 .. 47661477
3804 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0361000.1

Sequence Viewer

Length: 1020 bp
ATGAGTTGCATGACTGCAATTCTTGTAATTGCAATCTGTTTTGTTCAAGCCTTGTATGCAGGCCTCTCCATCATCACAATCCTTGCACTAGGGCAGTGCAAGAGCCACTACACCTCTGTGGTGTATCGTATGGCCATTACAACTGCCCTGGCAAGTCCATTGGCATGGTATCTAGAGAGAAATTCTAGGCCTACAATGACCATCAAGGTCATGGCAAAGATCATGCTGCTAAGCATGTTTGACCCTGTAATGAACTTAAACTTATACTATGCGGGCATGGATAACTCCAATGCCACATTCACATCTGCCATGTGCAACATGCTTCCGGTGTTAGCATTTGTCATGGCCTGGATTTTCAGGCTGGAGAATGTGGACTACAGACACCCGAGAGGATTGGCCAAGGTTCTAGGAACCTTGGTTACTCTGGTAGGAGCAATCCTCTTTACCATGGTCAAGGGGCCTTCCCTCACCTTGCCATGGGCAAGGGACCACGAGAAAAATCATTCTTCACAGCCCAAAGTCAAGGGTGCTCTATTTCTAACACTAGCCTGTTTCTGCTGCTCTTGTTTCATGATCCTACAAGACAATGTACTCAAGTCCTACCCCTGCAAGCTCTCTCTCACCGCTTTGATTTGCTTCTGGGGTATGGTGGAAGGAGCAGTGGTGGCCGTTGTGGTTGAAAGGGGGGATTCGGAAGCATGGTCCATACACTTGGACTTCAAGTTGCTCGCAGCTGTTTATGGGGCTCTTCTATCGGGGGCTACATATTATGTCATGGGACTGGTTGTGAAGAAGAAGGGTCCAGTTTTTTACTGGGCCTTTAACCCCTTGGCCACTCTGCTTGTGGCGATTTTGGGGCCTTTTTTTCTGGCACAACAATTGCATACTGGGAGTCTCATCGGAGCTATAACCATTGTGGGAGGCCTCTATCTTGTTCTTTGGGGAAAATCAAGAGATCAGCCTTCCTCCGATTCAAAGAATGCCAATGTGAAAGAACCAACTGGAACCCGCCAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
ATG27

Protein Analysis

339

Amino Acids

37.15

Weight (kDa)

9.18

Isoelectric Point (pI)

28.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 10 - 149 3.1e-08 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010981)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40316 AT2G40316 AT2G40316
fragaria_vesca FvH4_7g25140 FvH4_7g25140
malus_domestica MD01G1158200.v1.1 MD07G1226900.v1.1
prunus_persica Prupe.2G256500_v2.0.a1
pyrus_communis pycom01g17490 pycom07g20550
rosa_chinensis RchiOBHm_Chr1g0371041
rosa_laevigata RLG00000026990
rosa_multiflora Rmu_sc0001018.1_g000028
rosa_roxburghii Rroxscaffold_4G00286040
rosa_rugosa Rorug01G0361000 Rorug01G0361100
rosa_samantha Rh1AG371300 Rh1CG348100 Rh1DG366000
rosa_wichuraiana Rw1G032660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 272, 624, 1009
AclWI GGATC 1 cut(s) 568
AcoI YGGCCR 4 cut(s) 132, 396, 666, 831
AcsI RAATTY 1 cut(s) 181
AfaI GTAC 1 cut(s) 591
AfiI CCNNNNNNNGG 1 cut(s) 477
AgsI TTSAA 4 cut(s) 47, 680, 721, 975
AjnI CCWGG 2 cut(s) 147, 347
AjuI GAANNNNNNNTTGG 2 cut(s) 281, 313
AleI CACNNNNGTG 1 cut(s) 116
AluBI AGCT 3 cut(s) 613, 734, 905
AluI AGCT 3 cut(s) 613, 734, 905
Alw21I GWGCWC 1 cut(s) 532
Alw26I GTCTC 1 cut(s) 899
AlwI GGATC 1 cut(s) 568
Ama87I CYCGRG 1 cut(s) 385
ApeKI GCWGC 3 cut(s) 226, 558, 731
ApoI RAATTY 1 cut(s) 181
AspS9I GGNCC 6 cut(s) 458, 487, 702, 800, 816, 857
AsuHPI GGTGA 2 cut(s) 460, 613
AvaI CYCGRG 1 cut(s) 385
AvaII GGWCC 3 cut(s) 487, 702, 800
BalI TGGCCA 3 cut(s) 134, 398, 833
BanII GRGCYC 1 cut(s) 748
BauI CACGAG 1 cut(s) 491
Bbv12I GWGCWC 1 cut(s) 532
BbvI GCAGC 3 cut(s) 213, 545, 743
BccI CCATC 2 cut(s) 77, 209
BceAI ACGGC 1 cut(s) 653
BciT130I CCWGG 2 cut(s) 149, 349
BcoDI GTCTC 1 cut(s) 899
BfaI CTAG 5 cut(s) 89, 173, 186, 407, 545
BfmI CTRYAG 1 cut(s) 376
BisI GCNGC 3 cut(s) 227, 559, 732
BlpI GCTNAGC 1 cut(s) 230
BlsI GCNGC 3 cut(s) 228, 560, 733
Bme1390I CCNGG 2 cut(s) 149, 349
Bme18I GGWCC 3 cut(s) 487, 702, 800
BmeT110I CYCGRG 1 cut(s) 385
BmgT120I GGNCC 6 cut(s) 458, 487, 702, 800, 816, 857
BmiI GGNNCC 6 cut(s) 412, 459, 488, 801, 858, 1006
BmrFI CCNGG 2 cut(s) 149, 349
BmrI ACTGGG 2 cut(s) 823, 897
BmuI ACTGGG 2 cut(s) 823, 897
BplI GAGNNNNNCTC 2 cut(s) 423, 455
BpmI CTGGAG 1 cut(s) 383
Bpu1102I GCTNAGC 1 cut(s) 230
BpuEI CTTGAG 1 cut(s) 578
BsaJI CCNNGG 6 cut(s) 147, 399, 414, 447, 476, 828
BsaWI WCCGGW 1 cut(s) 325
BsaXI ACNNNNNCTCC 2 cut(s) 648, 678
Bsc4I CCNNNNNNNGG 1 cut(s) 477
Bse1I ACTGG 5 cut(s) 786, 803, 818, 892, 1006
BseBI CCWGG 2 cut(s) 149, 349
BseDI CCNNGG 6 cut(s) 147, 399, 414, 447, 476, 828
BseLI CCNNNNNNNGG 1 cut(s) 477
BseNI ACTGG 5 cut(s) 786, 803, 818, 892, 1006
BseXI GCAGC 3 cut(s) 213, 545, 743
BsiHKAI GWGCWC 1 cut(s) 532
BsiHKCI CYCGRG 1 cut(s) 385
BsiSI CCGG 1 cut(s) 326
BslFI GGGAC 2 cut(s) 500, 792
BslI CCNNNNNNNGG 1 cut(s) 477
BsmAI GTCTC 1 cut(s) 899
BsmFI GGGAC 2 cut(s) 500, 792
BsmI GAATGC 1 cut(s) 985
BsoBI CYCGRG 1 cut(s) 385
Bsp1286I GDGCHC 2 cut(s) 532, 748
Bsp143I GATC 3 cut(s) 219, 573, 955
Bsp1720I GCTNAGC 1 cut(s) 230
Bsp19I CCATGG 2 cut(s) 447, 476
BspACI CCGC 3 cut(s) 272, 624, 1009
BspHI TCATGA 1 cut(s) 570
BspLI GGNNCC 6 cut(s) 412, 459, 488, 801, 858, 1006
BspPI GGATC 1 cut(s) 568
BspQI GCTCTTC 1 cut(s) 753
BsrI ACTGG 5 cut(s) 786, 803, 818, 892, 1006
BssECI CCNNGG 6 cut(s) 147, 399, 414, 447, 476, 828
BssMI GATC 3 cut(s) 219, 573, 955
BssSI CACGAG 1 cut(s) 491
BssT1I CCWWGG 5 cut(s) 399, 414, 447, 476, 828
Bst2BI CACGAG 1 cut(s) 491
Bst2UI CCWGG 2 cut(s) 149, 349
Bst6I CTCTTC 1 cut(s) 753
BstC8I GCNNGC 4 cut(s) 61, 274, 611, 729
BstDEI CTNAG 1 cut(s) 230
BstDSI CCRYGG 2 cut(s) 447, 476
BstKTI GATC 3 cut(s) 222, 576, 958
BstMAI GTCTC 1 cut(s) 899
BstMBI GATC 3 cut(s) 219, 573, 955
BstMWI GCNNNNNNNGC 2 cut(s) 56, 665
BstNI CCWGG 2 cut(s) 149, 349
BstNSI RCATGY 2 cut(s) 238, 322
BstSCI CCNGG 2 cut(s) 147, 347
BstSFI CTRYAG 1 cut(s) 376
BstV1I GCAGC 3 cut(s) 213, 545, 743
BstXI CCANNNNNNTGG 2 cut(s) 165, 712
BtgI CCRYGG 2 cut(s) 447, 476
BtsI GCAGTG 2 cut(s) 101, 666
BtsIMutI CAGTG 2 cut(s) 101, 666
Cac8I GCNNGC 4 cut(s) 61, 274, 611, 729
CciI TCATGA 1 cut(s) 570
Cfr13I GGNCC 6 cut(s) 458, 487, 702, 800, 816, 857
Csp6I GTAC 1 cut(s) 590
CspCI CAANNNNNGTGG 2 cut(s) 823, 858
CviQI GTAC 1 cut(s) 590
DdeI CTNAG 1 cut(s) 230
DpnI GATC 3 cut(s) 221, 575, 957
DpnII GATC 3 cut(s) 219, 573, 955
EaeI YGGCCR 4 cut(s) 132, 396, 666, 831
Eam1104I CTCTTC 1 cut(s) 753
EarI CTCTTC 1 cut(s) 753
Eco130I CCWWGG 5 cut(s) 399, 414, 447, 476, 828
Eco147I AGGCCT 3 cut(s) 63, 190, 924
Eco24I GRGCYC 1 cut(s) 748
Eco47I GGWCC 3 cut(s) 487, 702, 800
Eco88I CYCGRG 1 cut(s) 385
EcoO109I RGGNCCY 2 cut(s) 458, 857
EcoRII CCWGG 2 cut(s) 147, 347
EcoT14I CCWWGG 5 cut(s) 399, 414, 447, 476, 828
EcoT38I GRGCYC 1 cut(s) 748
ErhI CCWWGG 5 cut(s) 399, 414, 447, 476, 828
FaqI GGGAC 2 cut(s) 500, 792
FauI CCCGC 2 cut(s) 265, 1016
Fnu4HI GCNGC 3 cut(s) 227, 559, 732
FriOI GRGCYC 1 cut(s) 748
Fsp4HI GCNGC 3 cut(s) 227, 559, 732
FspBI CTAG 5 cut(s) 89, 173, 186, 407, 545
GluI GCNGC 3 cut(s) 227, 559, 732
GsuI CTGGAG 1 cut(s) 383
HapII CCGG 1 cut(s) 326
HinfI GANTC 3 cut(s) 689, 892, 971
HpaII CCGG 1 cut(s) 326
HphI GGTGA 2 cut(s) 460, 613
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 3 cut(s) 694, 902, 970
Hpy188III TCNNGA 3 cut(s) 173, 571, 951
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 4 cut(s) 471, 647, 790, 972
HpyCH4V TGCA 9 cut(s) 9, 17, 32, 59, 86, 99, 315, 609, 883
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 665
HpyF3I CTNAG 1 cut(s) 230
Kzo9I GATC 3 cut(s) 219, 573, 955
LguI GCTCTTC 1 cut(s) 753
LmnI GCTCC 3 cut(s) 431, 656, 902
Lsp1109I GCAGC 3 cut(s) 213, 545, 743
MaeI CTAG 5 cut(s) 89, 173, 186, 407, 545
MaeIII GTNAC 1 cut(s) 418
MalI GATC 3 cut(s) 221, 575, 957
MboI GATC 3 cut(s) 219, 573, 955
MboII GAAGA 4 cut(s) 498, 740, 802, 805
MfeI CAATTG 1 cut(s) 878
MhlI GDGCHC 2 cut(s) 532, 748
MlsI TGGCCA 3 cut(s) 134, 398, 833
MluCI AATT 4 cut(s) 18, 27, 181, 878
MluNI TGGCCA 3 cut(s) 134, 398, 833
MlyI GAGTC 1 cut(s) 901
MnlI CCTC 8 cut(s) 74, 124, 383, 449, 476, 914, 935, 976
Mox20I TGGCCA 3 cut(s) 134, 398, 833
MscI TGGCCA 3 cut(s) 134, 398, 833
MseI TTAA 3 cut(s) 257, 822, 1018
MslI CAYNNNNRTG 2 cut(s) 116, 163
Msp20I TGGCCA 3 cut(s) 134, 398, 833
MspA1I CMGCKG 1 cut(s) 734
MspI CCGG 1 cut(s) 326
MspR9I CCNGG 2 cut(s) 149, 349
MunI CAATTG 1 cut(s) 878
Mva1269I GAATGC 1 cut(s) 985
MvaI CCWGG 2 cut(s) 149, 349
MwoI GCNNNNNNNGC 2 cut(s) 56, 665
NcoI CCATGG 2 cut(s) 447, 476
NdeII GATC 3 cut(s) 219, 573, 955
NlaIV GGNNCC 6 cut(s) 412, 459, 488, 801, 858, 1006
NspI RCATGY 2 cut(s) 238, 322
OliI CACNNNNGTG 1 cut(s) 116
PagI TCATGA 1 cut(s) 570
PceI AGGCCT 3 cut(s) 63, 190, 924
PciSI GCTCTTC 1 cut(s) 753
PctI GAATGC 1 cut(s) 985
PfeI GAWTC 2 cut(s) 689, 971
PkrI GCNGC 3 cut(s) 228, 560, 733
PleI GAGTC 1 cut(s) 900
PpsI GAGTC 1 cut(s) 900
Psp6I CCWGG 2 cut(s) 147, 347
PspGI CCWGG 2 cut(s) 147, 347
PspN4I GGNNCC 6 cut(s) 412, 459, 488, 801, 858, 1006
PspPI GGNCC 6 cut(s) 458, 487, 702, 800, 816, 857
PsrI GAACNNNNNNTAC 2 cut(s) 403, 435
PvuII CAGCTG 1 cut(s) 734
RsaI GTAC 1 cut(s) 591
RsaNI GTAC 1 cut(s) 590
RseI CAYNNNNRTG 2 cut(s) 116, 163
SapI GCTCTTC 1 cut(s) 753
SaqAI TTAA 3 cut(s) 257, 822, 1018
SatI GCNGC 3 cut(s) 227, 559, 732
Sau3AI GATC 3 cut(s) 219, 573, 955
Sau96I GGNCC 6 cut(s) 458, 487, 702, 800, 816, 857
SchI GAGTC 1 cut(s) 901
ScrFI CCNGG 2 cut(s) 149, 349
SduI GDGCHC 2 cut(s) 532, 748
SetI ASST 8 cut(s) 116, 210, 405, 416, 473, 615, 736, 907
SfcI CTRYAG 1 cut(s) 376
SinI GGWCC 3 cut(s) 487, 702, 800
SmiMI CAYNNNNRTG 2 cut(s) 116, 163
SmlI CTYRAG 1 cut(s) 593
SmoI CTYRAG 1 cut(s) 593
Sse9I AATT 4 cut(s) 18, 27, 181, 878
SseBI AGGCCT 3 cut(s) 63, 190, 924
SsiI CCGC 3 cut(s) 272, 624, 1009
SspMI CTAG 5 cut(s) 89, 173, 186, 407, 545
StuI AGGCCT 3 cut(s) 63, 190, 924
StyD4I CCNGG 2 cut(s) 147, 347
StyI CCWWGG 5 cut(s) 399, 414, 447, 476, 828
TasI AATT 4 cut(s) 18, 27, 181, 878
TatI WGTACW 1 cut(s) 589
TfiI GAWTC 2 cut(s) 689, 971
Tru1I TTAA 3 cut(s) 257, 822, 1018
Tru9I TTAA 3 cut(s) 257, 822, 1018
TscAI CASTG 2 cut(s) 101, 666
TseI GCWGC 3 cut(s) 226, 558, 731
TspDTI ATGAA 2 cut(s) 266, 559
TspRI CASTG 2 cut(s) 101, 666
VpaK11BI GGWCC 3 cut(s) 487, 702, 800
XapI RAATTY 1 cut(s) 181
XbaI TCTAGA 1 cut(s) 172
XceI RCATGY 2 cut(s) 238, 322
XcmI CCANNNNNNNNNTGG 3 cut(s) 208, 810, 841
XspI CTAG 5 cut(s) 89, 173, 186, 407, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.