Rw1G032660

Autophagy-related protein 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0010981
Physical Location & Seq
Forward (+)
60907613 .. 60913427
5815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G032660.1

Sequence Viewer

Length: 825 bp
ATGGTGTTCCGAGGCGATTCTCTAAAATGGGTTGTAGTCGCCTTGACGATTCTGCACCCGGTTGACCCGCTCTCTGCCAACTGCCATTTCAGTTTTGTTGCCGATTACAAGCTCTACGACTTCAGTTTGGATACTCCCATACCCTTGTATCCTCACGGAGTTCAAAGCGAAGATGGGTTCTACAAGGTCGTAGGAAATAAGACTGTGATCTGGTTTCAGCTTTGCGATGGAATGATTTTCAATCACGACCCACCTAGATGTGTTGATTGCTCGGATTGTGGTGGCCCATCACACTGTGGCATGGGTTGTAGTGCTCTTGTAGCAAAAAACACAGGAGGTTACGATGTTTGCACTAACATTGGGCGTGCCTCGAGCATGGACATTAACATAATTGATAAGAACAATCCTCACATTGGTGTCATTGTTAAGATGTCAAGTAATGGCCTAAATGAAAATTGTTCACTCTCTGTGTCTGTAATTTGTGATCCTTATGGATTTCAGGGACCATATTCGTTGGAGAAAACAGGGACATGTAACTATGGTACAGTGCTGAAGCATCCTTCTGGCTGTGCCAAGATTGTACATGTTAGTGGCAAAGGATGGGGCTGGTTTGCCACCTTTCTAATAATAGTCTTGTGCCTTTTTGGAGTGTATCTGTTGGCTGGTGCAGTTTATCGATATTACAGTCTTGGAGTTAGTGGAATAGATATTATACCAAATCTGGACTTTTGGACTGGCTTACCTCAGCAAGCACAAGTATTCTATTATTTAATCTGTTTTATCTTGCTCTTTGTTCTGTACTTCATTGCTGTCTCGCGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
ATG27

Protein Analysis

274

Amino Acids

30.09

Weight (kDa)

6.2

Isoelectric Point (pI)

26.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG27 PF09451 15 - 249 4.3e-18 Autophagy-related protein 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010981)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40316 AT2G40316 AT2G40316
fragaria_vesca FvH4_7g25140 FvH4_7g25140
malus_domestica MD01G1158200.v1.1 MD07G1226900.v1.1
prunus_persica Prupe.2G256500_v2.0.a1
pyrus_communis pycom01g17490 pycom07g20550
rosa_chinensis RchiOBHm_Chr1g0371041
rosa_laevigata RLG00000026990
rosa_multiflora Rmu_sc0001018.1_g000028
rosa_roxburghii Rroxscaffold_4G00286040
rosa_rugosa Rorug01G0361000 Rorug01G0361100
rosa_samantha Rh1AG371300 Rh1CG348100 Rh1DG366000
rosa_wichuraiana Rw1G032660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 70
AccII CGCG 1 cut(s) 817
AciI CCGC 2 cut(s) 68, 817
AclWI GGATC 1 cut(s) 479
AcuI CTGAAG 2 cut(s) 106, 572
AdeI CACNNNGTG 1 cut(s) 296
AfaI GTAC 3 cut(s) 544, 582, 800
AfiI CCNNNNNNNGG 1 cut(s) 413
AflIII ACRYGT 2 cut(s) 530, 583
AgsI TTSAA 2 cut(s) 164, 241
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AleI CACNNNNGTG 1 cut(s) 414
AluBI AGCT 2 cut(s) 112, 220
AluI AGCT 2 cut(s) 112, 220
Alw21I GWGCWC 1 cut(s) 316
Alw26I GTCTC 1 cut(s) 817
AlwI GGATC 1 cut(s) 479
Ama87I CYCGRG 1 cut(s) 370
AoxI GGCC 2 cut(s) 283, 442
AspS9I GGNCC 2 cut(s) 284, 503
AsuC2I CCSGG 1 cut(s) 59
AvaI CYCGRG 1 cut(s) 370
AvaII GGWCC 1 cut(s) 503
BaeI ACNNNNGTAYC 2 cut(s) 131, 164
Bbv12I GWGCWC 1 cut(s) 316
BbvCI CCTCAGC 1 cut(s) 744
BccI CCATC 4 cut(s) 167, 221, 295, 594
BciVI GTATCC 2 cut(s) 124, 159
BcnI CCSGG 1 cut(s) 59
BcoDI GTCTC 1 cut(s) 817
BfaI CTAG 1 cut(s) 255
BfuI GTATCC 2 cut(s) 124, 159
Bme1390I CCNGG 1 cut(s) 59
Bme18I GGWCC 1 cut(s) 503
BmeT110I CYCGRG 1 cut(s) 370
BmgT120I GGNCC 2 cut(s) 284, 503
BmiI GGNNCC 1 cut(s) 504
BmrFI CCNGG 1 cut(s) 59
BmsI GCATC 1 cut(s) 565
Bpu10I CCTNAGC 1 cut(s) 744
BpuMI CCSGG 1 cut(s) 59
Bsa29I ATCGAT 1 cut(s) 676
BsaJI CCNNGG 1 cut(s) 10
Bsc4I CCNNNNNNNGG 1 cut(s) 413
Bse1I ACTGG 1 cut(s) 739
Bse3DI GCAATG 1 cut(s) 804
BseCI ATCGAT 1 cut(s) 676
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 2 cut(s) 556, 605
BseLI CCNNNNNNNGG 1 cut(s) 413
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 1 cut(s) 758
BseNI ACTGG 1 cut(s) 739
BsgI GTGCAG 2 cut(s) 38, 687
Bsh1236I CGCG 1 cut(s) 817
BshFI GGCC 2 cut(s) 285, 444
BshVI ATCGAT 1 cut(s) 676
BsiHKAI GWGCWC 1 cut(s) 316
BsiHKCI CYCGRG 1 cut(s) 370
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 2 cut(s) 516, 541
BslI CCNNNNNNNGG 1 cut(s) 413
BsmAI GTCTC 1 cut(s) 817
BsmFI GGGAC 2 cut(s) 516, 541
BsnI GGCC 2 cut(s) 285, 444
BsoBI CYCGRG 1 cut(s) 370
Bsp1286I GDGCHC 1 cut(s) 316
Bsp1407I TGTACA 1 cut(s) 580
Bsp143I GATC 2 cut(s) 207, 484
BspACI CCGC 2 cut(s) 68, 817
BspANI GGCC 2 cut(s) 285, 444
BspCNI CTCAG 1 cut(s) 757
BspDI ATCGAT 1 cut(s) 676
BspFNI CGCG 1 cut(s) 817
BspLI GGNNCC 1 cut(s) 504
BspPI GGATC 1 cut(s) 479
BsrBI CCGCTC 1 cut(s) 70
BsrDI GCAATG 1 cut(s) 804
BsrGI TGTACA 1 cut(s) 580
BsrI ACTGG 1 cut(s) 739
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 2 cut(s) 207, 484
Bst4CI ACNGT 4 cut(s) 205, 296, 547, 686
BstAUI TGTACA 1 cut(s) 580
BstC8I GCNNGC 2 cut(s) 366, 750
BstDEI CTNAG 1 cut(s) 744
BstF5I GGATG 2 cut(s) 556, 605
BstFNI CGCG 1 cut(s) 817
BstKTI GATC 2 cut(s) 210, 487
BstMAI GTCTC 1 cut(s) 817
BstMBI GATC 2 cut(s) 207, 484
BstMWI GCNNNNNNNGC 1 cut(s) 320
BstNSI RCATGY 2 cut(s) 534, 587
BstSCI CCNGG 1 cut(s) 57
BstUI CGCG 1 cut(s) 817
Bsu15I ATCGAT 1 cut(s) 676
BsuI GTATCC 2 cut(s) 124, 159
BsuRI GGCC 2 cut(s) 285, 444
BsuTUI ATCGAT 1 cut(s) 676
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 2 cut(s) 556, 605
BtsIMutI CAGTG 2 cut(s) 292, 552
Cac8I GCNNGC 2 cut(s) 366, 750
Cfr13I GGNCC 2 cut(s) 284, 503
ClaI ATCGAT 1 cut(s) 676
Csp6I GTAC 3 cut(s) 543, 581, 799
CviAII CATG 4 cut(s) 301, 376, 531, 584
CviJI RGCY 8 cut(s) 112, 220, 285, 444, 567, 606, 662, 738
CviKI_1 RGCY 8 cut(s) 112, 220, 285, 444, 567, 606, 662, 738
CviQI GTAC 3 cut(s) 543, 581, 799
DdeI CTNAG 1 cut(s) 744
DpnI GATC 2 cut(s) 209, 486
DpnII GATC 2 cut(s) 207, 484
DraIII CACNNNGTG 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 503
Eco57I CTGAAG 2 cut(s) 106, 572
Eco88I CYCGRG 1 cut(s) 370
FaeI CATG 4 cut(s) 304, 379, 534, 587
FaqI GGGAC 2 cut(s) 516, 541
FatI CATG 4 cut(s) 300, 375, 530, 583
FauI CCCGC 1 cut(s) 75
FokI GGATG 2 cut(s) 543, 612
FspBI CTAG 1 cut(s) 255
HaeIII GGCC 2 cut(s) 285, 444
HapII CCGG 1 cut(s) 59
Hin1II CATG 4 cut(s) 304, 379, 534, 587
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HinfI GANTC 2 cut(s) 17, 49
HpaII CCGG 1 cut(s) 59
Hpy166II GTNNAC 2 cut(s) 64, 461
Hpy188I TCNGA 2 cut(s) 11, 274
Hpy188III TCNNGA 2 cut(s) 245, 722
Hpy8I GTNNAC 2 cut(s) 64, 461
HpyAV CCTTC 1 cut(s) 570
HpyCH4III ACNGT 4 cut(s) 205, 296, 547, 686
HpyCH4V TGCA 3 cut(s) 55, 351, 668
HpyF10VI GCNNNNNNNGC 1 cut(s) 320
HpyF3I CTNAG 1 cut(s) 744
Hsp92II CATG 4 cut(s) 304, 379, 534, 587
Kzo9I GATC 2 cut(s) 207, 484
LweI GCATC 1 cut(s) 565
MaeI CTAG 1 cut(s) 255
MaeIII GTNAC 2 cut(s) 338, 533
MalI GATC 2 cut(s) 209, 486
MbiI CCGCTC 1 cut(s) 70
MboI GATC 2 cut(s) 207, 484
MboII GAAGA 1 cut(s) 182
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 3 cut(s) 390, 454, 477
MmeI TCCRAC 1 cut(s) 495
MnlI CCTC 6 cut(s) 5, 162, 329, 379, 417, 753
MseI TTAA 3 cut(s) 384, 426, 770
MslI CAYNNNNRTG 3 cut(s) 256, 414, 588
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 1 cut(s) 59
MvnI CGCG 1 cut(s) 817
MwoI GCNNNNNNNGC 1 cut(s) 320
NciI CCSGG 1 cut(s) 59
NdeII GATC 2 cut(s) 207, 484
NlaIII CATG 4 cut(s) 304, 379, 534, 587
NlaIV GGNNCC 1 cut(s) 504
NspI RCATGY 2 cut(s) 534, 587
OliI CACNNNNGTG 1 cut(s) 414
PaeR7I CTCGAG 1 cut(s) 370
PciI ACATGT 2 cut(s) 530, 583
PfeI GAWTC 2 cut(s) 17, 49
PscI ACATGT 2 cut(s) 530, 583
PspN4I GGNNCC 1 cut(s) 504
PspPI GGNCC 2 cut(s) 284, 503
PspXI VCTCGAGB 1 cut(s) 370
RsaI GTAC 3 cut(s) 544, 582, 800
RsaNI GTAC 3 cut(s) 543, 581, 799
RseI CAYNNNNRTG 3 cut(s) 256, 414, 588
SaqAI TTAA 3 cut(s) 384, 426, 770
Sau3AI GATC 2 cut(s) 207, 484
Sau96I GGNCC 2 cut(s) 284, 503
ScrFI CCNGG 1 cut(s) 59
SduI GDGCHC 1 cut(s) 316
SetI ASST 7 cut(s) 114, 189, 222, 256, 340, 620, 745
SfaNI GCATC 1 cut(s) 565
Sfr274I CTCGAG 1 cut(s) 370
SinI GGWCC 1 cut(s) 503
SlaI CTCGAG 1 cut(s) 370
SmiMI CAYNNNNRTG 3 cut(s) 256, 414, 588
SmlI CTYRAG 1 cut(s) 370
SmoI CTYRAG 1 cut(s) 370
Sse9I AATT 3 cut(s) 390, 454, 477
SsiI CCGC 2 cut(s) 68, 817
SspMI CTAG 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 57
TaaI ACNGT 4 cut(s) 205, 296, 547, 686
TaqI TCGA 2 cut(s) 371, 676
TasI AATT 3 cut(s) 390, 454, 477
TatI WGTACW 2 cut(s) 580, 798
TfiI GAWTC 2 cut(s) 17, 49
Tru1I TTAA 3 cut(s) 384, 426, 770
Tru9I TTAA 3 cut(s) 384, 426, 770
TscAI CASTG 2 cut(s) 299, 552
TspDTI ATGAA 2 cut(s) 465, 793
TspGWI ACGGA 1 cut(s) 171
TspRI CASTG 2 cut(s) 299, 552
VpaK11BI GGWCC 1 cut(s) 503
XceI RCATGY 2 cut(s) 534, 587
XhoI CTCGAG 1 cut(s) 370
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.