Rh1CG348100

Autophagy-related protein 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0010981
Physical Location & Seq
Forward (+)
62508297 .. 62510372
2076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG348100.1

Sequence Viewer

Length: 543 bp
ATGATTTTCAATCACGACCCACCTAGATGTGTTGATTGCTCGGATTGTGGTGGCCCATCACACTGTGGCATGGGTTGTAGTGCTCTTGTAGCAAAAAACACAGGAGGTTACGATGTTTGCACTAACATTGGGCGTGCCTCGAGCATGGACATTAACATAATTGATAAGAACAATCCTCACATTGGTGTCATTGTTAAGATGTCAAGTAATGGCCTCAATGAAAATTGTTCACTCTCTGTGTCTGTAATTTGTGATCCTTATGGATTTCAGGGACCATATTCGTTGGAGAAAACAGGGACATGTAACTATGGTACAGTGCTGAAGCATCCTTCTGGCTGTGCCAAGATTGTACATGTTAGTGGCAAAGGATGGGGCTGGTTTGCCACCTTTCTAATAATAGTCTTGTGCCTTTTTGGAGTATATCTGTTGGCTGGTGCAGTTTATCGATATTACAGTCTTGGAGTTCGTGGAATAGATGTATGTCTTCTAACTCAATTGATCTGCTTCTTCCATGCAGCCAGTAGACGGTTTAGTTGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
ATG27

Protein Analysis

180

Amino Acids

19.34

Weight (kDa)

8.19

Isoelectric Point (pI)

29.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG27 PF09451 21 - 160 3.3e-10 Autophagy-related protein 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010981)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40316 AT2G40316 AT2G40316
fragaria_vesca FvH4_7g25140 FvH4_7g25140
malus_domestica MD01G1158200.v1.1 MD07G1226900.v1.1
prunus_persica Prupe.2G256500_v2.0.a1
pyrus_communis pycom01g17490 pycom07g20550
rosa_chinensis RchiOBHm_Chr1g0371041
rosa_laevigata RLG00000026990
rosa_multiflora Rmu_sc0001018.1_g000028
rosa_roxburghii Rroxscaffold_4G00286040
rosa_rugosa Rorug01G0361000 Rorug01G0361100
rosa_samantha Rh1AG371300 Rh1CG348100 Rh1DG366000
rosa_wichuraiana Rw1G032660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 523
AclWI GGATC 1 cut(s) 248
AcuI CTGAAG 1 cut(s) 341
AdeI CACNNNGTG 1 cut(s) 65
AfaI GTAC 3 cut(s) 313, 351, 538
AfiI CCNNNNNNNGG 2 cut(s) 182, 525
AflIII ACRYGT 2 cut(s) 299, 352
AgsI TTSAA 1 cut(s) 10
AleI CACNNNNGTG 1 cut(s) 183
Alw21I GWGCWC 1 cut(s) 85
AlwI GGATC 1 cut(s) 248
Ama87I CYCGRG 1 cut(s) 139
AoxI GGCC 2 cut(s) 52, 211
ApeKI GCWGC 1 cut(s) 515
AspS9I GGNCC 2 cut(s) 53, 272
AvaI CYCGRG 1 cut(s) 139
AvaII GGWCC 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 85
BbvI GCAGC 1 cut(s) 527
BccI CCATC 2 cut(s) 64, 363
BfaI CTAG 1 cut(s) 24
BisI GCNGC 1 cut(s) 516
BlsI GCNGC 1 cut(s) 517
Bme18I GGWCC 1 cut(s) 272
BmeT110I CYCGRG 1 cut(s) 139
BmgT120I GGNCC 2 cut(s) 53, 272
BmiI GGNNCC 1 cut(s) 273
BmsI GCATC 1 cut(s) 334
BpiI GAAGAC 1 cut(s) 476
Bsa29I ATCGAT 1 cut(s) 445
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 525
Bse1I ACTGG 1 cut(s) 519
BseCI ATCGAT 1 cut(s) 445
BseGI GGATG 2 cut(s) 325, 374
BseLI CCNNNNNNNGG 2 cut(s) 182, 525
BseNI ACTGG 1 cut(s) 519
BseXI GCAGC 1 cut(s) 527
BsgI GTGCAG 1 cut(s) 456
BshFI GGCC 2 cut(s) 54, 213
BshVI ATCGAT 1 cut(s) 445
BsiHKAI GWGCWC 1 cut(s) 85
BsiHKCI CYCGRG 1 cut(s) 139
BslFI GGGAC 2 cut(s) 285, 310
BslI CCNNNNNNNGG 2 cut(s) 182, 525
BsmFI GGGAC 2 cut(s) 285, 310
BsnI GGCC 2 cut(s) 54, 213
BsoBI CYCGRG 1 cut(s) 139
Bsp1286I GDGCHC 1 cut(s) 85
Bsp1407I TGTACA 1 cut(s) 349
Bsp143I GATC 2 cut(s) 253, 498
BspANI GGCC 2 cut(s) 54, 213
BspDI ATCGAT 1 cut(s) 445
BspLI GGNNCC 1 cut(s) 273
BspPI GGATC 1 cut(s) 248
BsrGI TGTACA 1 cut(s) 349
BsrI ACTGG 1 cut(s) 519
BssMI GATC 2 cut(s) 253, 498
Bst4CI ACNGT 4 cut(s) 65, 316, 455, 528
BstAUI TGTACA 1 cut(s) 349
BstC8I GCNNGC 1 cut(s) 135
BstF5I GGATG 2 cut(s) 325, 374
BstKTI GATC 2 cut(s) 256, 501
BstMBI GATC 2 cut(s) 253, 498
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstNSI RCATGY 2 cut(s) 303, 356
BstV1I GCAGC 1 cut(s) 527
BstV2I GAAGAC 1 cut(s) 476
Bsu15I ATCGAT 1 cut(s) 445
BsuRI GGCC 2 cut(s) 54, 213
BsuTUI ATCGAT 1 cut(s) 445
BtsCI GGATG 2 cut(s) 325, 374
BtsIMutI CAGTG 2 cut(s) 61, 321
Cac8I GCNNGC 1 cut(s) 135
Cfr13I GGNCC 2 cut(s) 53, 272
ClaI ATCGAT 1 cut(s) 445
Csp6I GTAC 3 cut(s) 312, 350, 537
CviAII CATG 5 cut(s) 70, 145, 300, 353, 512
CviJI RGCY 6 cut(s) 54, 213, 336, 375, 431, 518
CviKI_1 RGCY 6 cut(s) 54, 213, 336, 375, 431, 518
CviQI GTAC 3 cut(s) 312, 350, 537
DpnI GATC 2 cut(s) 255, 500
DpnII GATC 2 cut(s) 253, 498
DraIII CACNNNGTG 1 cut(s) 65
Eco47I GGWCC 1 cut(s) 272
Eco57I CTGAAG 1 cut(s) 341
Eco88I CYCGRG 1 cut(s) 139
FaeI CATG 5 cut(s) 73, 148, 303, 356, 515
FaqI GGGAC 2 cut(s) 285, 310
FatI CATG 5 cut(s) 69, 144, 299, 352, 511
FblI GTMKAC 1 cut(s) 523
Fnu4HI GCNGC 1 cut(s) 516
FokI GGATG 2 cut(s) 312, 381
Fsp4HI GCNGC 1 cut(s) 516
FspBI CTAG 1 cut(s) 24
GluI GCNGC 1 cut(s) 516
HaeIII GGCC 2 cut(s) 54, 213
Hin1II CATG 5 cut(s) 73, 148, 303, 356, 515
Hpy166II GTNNAC 2 cut(s) 230, 524
Hpy188I TCNGA 1 cut(s) 43
Hpy188III TCNNGA 1 cut(s) 14
Hpy8I GTNNAC 2 cut(s) 230, 524
HpyAV CCTTC 1 cut(s) 339
HpyCH4III ACNGT 4 cut(s) 65, 316, 455, 528
HpyCH4V TGCA 3 cut(s) 120, 437, 515
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
Hsp92II CATG 5 cut(s) 73, 148, 303, 356, 515
Kzo9I GATC 2 cut(s) 253, 498
LpnPI CCDG 7 cut(s) 87, 254, 279, 318, 361, 417, 532
Lsp1109I GCAGC 1 cut(s) 527
LweI GCATC 1 cut(s) 334
MaeI CTAG 1 cut(s) 24
MaeIII GTNAC 2 cut(s) 107, 302
MalI GATC 2 cut(s) 255, 500
MboI GATC 2 cut(s) 253, 498
MboII GAAGA 2 cut(s) 476, 499
MfeI CAATTG 1 cut(s) 494
MhlI GDGCHC 1 cut(s) 85
MluCI AATT 4 cut(s) 159, 223, 246, 494
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 4 cut(s) 98, 148, 186, 224
MseI TTAA 2 cut(s) 153, 195
MslI CAYNNNNRTG 3 cut(s) 25, 183, 357
MunI CAATTG 1 cut(s) 494
MwoI GCNNNNNNNGC 1 cut(s) 89
NdeII GATC 2 cut(s) 253, 498
NlaIII CATG 5 cut(s) 73, 148, 303, 356, 515
NlaIV GGNNCC 1 cut(s) 273
NspI RCATGY 2 cut(s) 303, 356
OliI CACNNNNGTG 1 cut(s) 183
PaeR7I CTCGAG 1 cut(s) 139
PciI ACATGT 2 cut(s) 299, 352
PkrI GCNGC 1 cut(s) 517
PscI ACATGT 2 cut(s) 299, 352
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 2 cut(s) 53, 272
PspXI VCTCGAGB 1 cut(s) 139
RsaI GTAC 3 cut(s) 313, 351, 538
RsaNI GTAC 3 cut(s) 312, 350, 537
RseI CAYNNNNRTG 3 cut(s) 25, 183, 357
SaqAI TTAA 2 cut(s) 153, 195
SatI GCNGC 1 cut(s) 516
Sau3AI GATC 2 cut(s) 253, 498
Sau96I GGNCC 2 cut(s) 53, 272
SduI GDGCHC 1 cut(s) 85
SetI ASST 3 cut(s) 25, 109, 389
SfaNI GCATC 1 cut(s) 334
Sfr274I CTCGAG 1 cut(s) 139
SinI GGWCC 1 cut(s) 272
SlaI CTCGAG 1 cut(s) 139
SmiMI CAYNNNNRTG 3 cut(s) 25, 183, 357
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
Sse9I AATT 4 cut(s) 159, 223, 246, 494
SspMI CTAG 1 cut(s) 24
TaaI ACNGT 4 cut(s) 65, 316, 455, 528
TaqI TCGA 2 cut(s) 140, 445
TasI AATT 4 cut(s) 159, 223, 246, 494
TatI WGTACW 2 cut(s) 349, 536
Tru1I TTAA 2 cut(s) 153, 195
Tru9I TTAA 2 cut(s) 153, 195
TscAI CASTG 2 cut(s) 68, 321
TseI GCWGC 1 cut(s) 515
TspDTI ATGAA 1 cut(s) 234
TspRI CASTG 2 cut(s) 68, 321
VpaK11BI GGWCC 1 cut(s) 272
XceI RCATGY 2 cut(s) 303, 356
XhoI CTCGAG 1 cut(s) 139
XmiI GTMKAC 1 cut(s) 523
XspI CTAG 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.