Rroxscaffold_4G00286040

Autophagy-related protein 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010981
Physical Location & Seq
Reverse (-)
7195217 .. 7199991
4775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286040.1

Sequence Viewer

Length: 852 bp
ATGGTGTTCCGAGGCGATTCTCTCAAATGGGTTGTAGTCGCCTTGACGATTCTGCACCCAGTTGACCCGGTCTCTGCCAACTGCCATTTCAGTTTTGTTGACGATTACAAGCTCTACGACTTCAGTTTGGATACTCCTATGCCCTTATACCCTCACGGAGTTCAAAGCGAAGATGGGTTCTACAAGGTCGTTGGAAATAAGACTGTGATCTGGTTTCAGCTTTGCGATGGAATGATTTTCAATCACGACCCACCTAGATGTGTTGATTGCTCGGATTGTGGTGGCCCATCACACTGTGGCATGGGTTGTAGTGCTCTTGTAGCAAAAAACACAGGAGGTTACGATGTTTGCACTAATATTGGGCGTGCCTCGAGCATGGACATTAACATAATTGATAAGAACAATCCTCACATTGGTGTCATTGTTAAGATGTCAATACCCAATAATGTCTGTAGATTGAAAAAATTTCTTACATGCCTGGTTCATGGTCTAGTTTCTAATCTTTTTATTTTGCAGGGACCATATTCGTTGGAGAAAACAGGGACATGTAACTATGGTACAGTGCTGAAGCATCCTTCTGGCTGTGCCAAGATTGTACATGTTAGTGGCAAAGGATGGGGCTGGTTTGCCACCTTTCTAATAATAGTCTTGTGCCTTTTTGGAGTGTATCTGTTGGCTGGTGCAGTTTATCGATATTACAGTCTTGGAGTTCGTGGAATAGATATTATACCAAATCTGGACTTTTGGACTGGCTTACCTCAGCAAGCACAGAGTGTGTCTGCTTCTTTATTGCACAAATTTAGGGGATCTTCTCAAGGTCATCGAAGCGTCTATTCTCCTGTCAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
ATG27

Protein Analysis

283

Amino Acids

31.08

Weight (kDa)

8.37

Isoelectric Point (pI)

32.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG27 PF09451 15 - 272 8.8e-17 Autophagy-related protein 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010981)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40316 AT2G40316 AT2G40316
fragaria_vesca FvH4_7g25140 FvH4_7g25140
malus_domestica MD01G1158200.v1.1 MD07G1226900.v1.1
prunus_persica Prupe.2G256500_v2.0.a1
pyrus_communis pycom01g17490 pycom07g20550
rosa_chinensis RchiOBHm_Chr1g0371041
rosa_laevigata RLG00000026990
rosa_multiflora Rmu_sc0001018.1_g000028
rosa_roxburghii Rroxscaffold_4G00286040
rosa_rugosa Rorug01G0361000 Rorug01G0361100
rosa_samantha Rh1AG371300 Rh1CG348100 Rh1DG366000
rosa_wichuraiana Rw1G032660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 814
AcsI RAATTY 2 cut(s) 464, 797
AcuI CTGAAG 2 cut(s) 106, 587
AdeI CACNNNGTG 2 cut(s) 296, 773
AfaI GTAC 2 cut(s) 559, 597
AfiI CCNNNNNNNGG 1 cut(s) 413
AflIII ACRYGT 2 cut(s) 545, 598
AgsI TTSAA 3 cut(s) 164, 241, 460
AjnI CCWGG 1 cut(s) 477
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AleI CACNNNNGTG 1 cut(s) 414
AluBI AGCT 2 cut(s) 112, 220
AluI AGCT 2 cut(s) 112, 220
Alw21I GWGCWC 1 cut(s) 316
Alw26I GTCTC 1 cut(s) 76
AlwI GGATC 1 cut(s) 814
Ama87I CYCGRG 1 cut(s) 370
AoxI GGCC 1 cut(s) 283
ApoI RAATTY 2 cut(s) 464, 797
AspS9I GGNCC 2 cut(s) 284, 518
AsuC2I CCSGG 1 cut(s) 68
AvaI CYCGRG 1 cut(s) 370
AvaII GGWCC 1 cut(s) 518
Bbv12I GWGCWC 1 cut(s) 316
BbvCI CCTCAGC 1 cut(s) 759
BccI CCATC 4 cut(s) 167, 221, 295, 609
BciT130I CCWGG 1 cut(s) 479
BciVI GTATCC 1 cut(s) 124
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 1 cut(s) 76
BfaI CTAG 2 cut(s) 255, 491
BfmI CTRYAG 1 cut(s) 451
BfuI GTATCC 1 cut(s) 124
Bme1390I CCNGG 2 cut(s) 68, 479
Bme18I GGWCC 1 cut(s) 518
BmeT110I CYCGRG 1 cut(s) 370
BmgT120I GGNCC 2 cut(s) 284, 518
BmiI GGNNCC 1 cut(s) 519
BmrFI CCNGG 2 cut(s) 68, 479
BmrI ACTGGG 1 cut(s) 53
BmsI GCATC 1 cut(s) 580
BmuI ACTGGG 1 cut(s) 53
Bpu10I CCTNAGC 1 cut(s) 759
BpuEI CTTGAG 1 cut(s) 798
BpuMI CCSGG 1 cut(s) 68
Bsa29I ATCGAT 1 cut(s) 691
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 1 cut(s) 10
Bsc4I CCNNNNNNNGG 1 cut(s) 413
Bse1I ACTGG 2 cut(s) 59, 754
BseBI CCWGG 1 cut(s) 479
BseCI ATCGAT 1 cut(s) 691
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 2 cut(s) 571, 620
BseLI CCNNNNNNNGG 1 cut(s) 413
BseMII CTCAG 1 cut(s) 773
BseNI ACTGG 2 cut(s) 59, 754
BsgI GTGCAG 2 cut(s) 38, 702
BshFI GGCC 1 cut(s) 285
BshVI ATCGAT 1 cut(s) 691
BsiHKAI GWGCWC 1 cut(s) 316
BsiHKCI CYCGRG 1 cut(s) 370
BsiSI CCGG 1 cut(s) 68
BslFI GGGAC 2 cut(s) 531, 556
BslI CCNNNNNNNGG 1 cut(s) 413
BsmAI GTCTC 1 cut(s) 76
BsmFI GGGAC 2 cut(s) 531, 556
BsnI GGCC 1 cut(s) 285
Bso31I GGTCTC 1 cut(s) 76
BsoBI CYCGRG 1 cut(s) 370
Bsp1286I GDGCHC 1 cut(s) 316
Bsp1407I TGTACA 1 cut(s) 595
Bsp143I GATC 2 cut(s) 207, 806
BspANI GGCC 1 cut(s) 285
BspCNI CTCAG 1 cut(s) 772
BspDI ATCGAT 1 cut(s) 691
BspLI GGNNCC 1 cut(s) 519
BspPI GGATC 1 cut(s) 814
BspTNI GGTCTC 1 cut(s) 76
BsrGI TGTACA 1 cut(s) 595
BsrI ACTGG 2 cut(s) 59, 754
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 2 cut(s) 207, 806
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 4 cut(s) 205, 296, 562, 701
BstAUI TGTACA 1 cut(s) 595
BstC8I GCNNGC 2 cut(s) 366, 765
BstDEI CTNAG 1 cut(s) 759
BstF5I GGATG 2 cut(s) 571, 620
BstKTI GATC 2 cut(s) 210, 809
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 2 cut(s) 207, 806
BstMWI GCNNNNNNNGC 1 cut(s) 320
BstNI CCWGG 1 cut(s) 479
BstNSI RCATGY 3 cut(s) 477, 549, 602
BstSCI CCNGG 2 cut(s) 66, 477
BstSFI CTRYAG 1 cut(s) 451
BstX2I RGATCY 1 cut(s) 806
BstYI RGATCY 1 cut(s) 806
Bsu15I ATCGAT 1 cut(s) 691
BsuI GTATCC 1 cut(s) 124
BsuRI GGCC 1 cut(s) 285
BsuTUI ATCGAT 1 cut(s) 691
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 2 cut(s) 571, 620
BtsIMutI CAGTG 2 cut(s) 292, 567
Cac8I GCNNGC 2 cut(s) 366, 765
Cfr13I GGNCC 2 cut(s) 284, 518
ClaI ATCGAT 1 cut(s) 691
CseI GACGC 1 cut(s) 817
Csp6I GTAC 2 cut(s) 558, 596
CviAII CATG 6 cut(s) 301, 376, 474, 485, 546, 599
CviJI RGCY 7 cut(s) 112, 220, 285, 582, 621, 677, 753
CviKI_1 RGCY 7 cut(s) 112, 220, 285, 582, 621, 677, 753
CviQI GTAC 2 cut(s) 558, 596
DdeI CTNAG 1 cut(s) 759
DpnI GATC 2 cut(s) 209, 808
DpnII GATC 2 cut(s) 207, 806
DraIII CACNNNGTG 2 cut(s) 296, 773
Eco31I GGTCTC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 518
Eco57I CTGAAG 2 cut(s) 106, 587
Eco88I CYCGRG 1 cut(s) 370
EcoRII CCWGG 1 cut(s) 477
FaeI CATG 6 cut(s) 304, 379, 477, 488, 549, 602
FaqI GGGAC 2 cut(s) 531, 556
FatI CATG 6 cut(s) 300, 375, 473, 484, 545, 598
FokI GGATG 2 cut(s) 558, 627
FspBI CTAG 2 cut(s) 255, 491
HaeIII GGCC 1 cut(s) 285
HapII CCGG 1 cut(s) 68
HgaI GACGC 1 cut(s) 817
Hin1II CATG 6 cut(s) 304, 379, 477, 488, 549, 602
HincII GTYRAC 3 cut(s) 64, 100, 844
HindII GTYRAC 3 cut(s) 64, 100, 844
HinfI GANTC 2 cut(s) 17, 49
HpaII CCGG 1 cut(s) 68
Hpy166II GTNNAC 3 cut(s) 64, 100, 844
Hpy188I TCNGA 3 cut(s) 11, 274, 851
Hpy188III TCNNGA 2 cut(s) 245, 737
Hpy8I GTNNAC 3 cut(s) 64, 100, 844
HpyAV CCTTC 1 cut(s) 585
HpyCH4III ACNGT 4 cut(s) 205, 296, 562, 701
HpyCH4V TGCA 5 cut(s) 55, 351, 514, 683, 793
HpyF10VI GCNNNNNNNGC 1 cut(s) 320
HpyF3I CTNAG 1 cut(s) 759
Hsp92II CATG 6 cut(s) 304, 379, 477, 488, 549, 602
Kzo9I GATC 2 cut(s) 207, 806
LweI GCATC 1 cut(s) 580
MaeI CTAG 2 cut(s) 255, 491
MaeIII GTNAC 2 cut(s) 338, 548
MalI GATC 2 cut(s) 209, 808
MboI GATC 2 cut(s) 207, 806
MboII GAAGA 2 cut(s) 182, 801
MflI RGATCY 1 cut(s) 806
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 3 cut(s) 390, 464, 797
MmeI TCCRAC 2 cut(s) 172, 510
MnlI CCTC 6 cut(s) 5, 162, 329, 379, 417, 768
MseI TTAA 2 cut(s) 384, 426
MslI CAYNNNNRTG 3 cut(s) 256, 414, 603
MspI CCGG 1 cut(s) 68
MspR9I CCNGG 2 cut(s) 68, 479
MvaI CCWGG 1 cut(s) 479
MwoI GCNNNNNNNGC 1 cut(s) 320
NciI CCSGG 1 cut(s) 68
NdeII GATC 2 cut(s) 207, 806
NlaIII CATG 6 cut(s) 304, 379, 477, 488, 549, 602
NlaIV GGNNCC 1 cut(s) 519
NspI RCATGY 3 cut(s) 477, 549, 602
OliI CACNNNNGTG 1 cut(s) 414
PaeR7I CTCGAG 1 cut(s) 370
PciI ACATGT 2 cut(s) 545, 598
PfeI GAWTC 2 cut(s) 17, 49
PflFI GACNNNGTC 1 cut(s) 68
PscI ACATGT 2 cut(s) 545, 598
Psp6I CCWGG 1 cut(s) 477
PspGI CCWGG 1 cut(s) 477
PspN4I GGNNCC 1 cut(s) 519
PspPI GGNCC 2 cut(s) 284, 518
PspXI VCTCGAGB 1 cut(s) 370
PsuI RGATCY 1 cut(s) 806
PsyI GACNNNGTC 1 cut(s) 68
RsaI GTAC 2 cut(s) 559, 597
RsaNI GTAC 2 cut(s) 558, 596
RseI CAYNNNNRTG 3 cut(s) 256, 414, 603
SaqAI TTAA 2 cut(s) 384, 426
Sau3AI GATC 2 cut(s) 207, 806
Sau96I GGNCC 2 cut(s) 284, 518
ScrFI CCNGG 2 cut(s) 68, 479
SduI GDGCHC 1 cut(s) 316
SetI ASST 8 cut(s) 114, 189, 222, 256, 340, 635, 760, 820
SfaNI GCATC 1 cut(s) 580
SfcI CTRYAG 1 cut(s) 451
Sfr274I CTCGAG 1 cut(s) 370
SinI GGWCC 1 cut(s) 518
SlaI CTCGAG 1 cut(s) 370
SmiMI CAYNNNNRTG 3 cut(s) 256, 414, 603
SmlI CTYRAG 2 cut(s) 370, 813
SmoI CTYRAG 2 cut(s) 370, 813
Sse9I AATT 3 cut(s) 390, 464, 797
SspI AATATT 1 cut(s) 358
SspMI CTAG 2 cut(s) 255, 491
StyD4I CCNGG 2 cut(s) 66, 477
TaaI ACNGT 4 cut(s) 205, 296, 562, 701
TaqI TCGA 3 cut(s) 371, 691, 823
TasI AATT 3 cut(s) 390, 464, 797
TatI WGTACW 1 cut(s) 595
TfiI GAWTC 2 cut(s) 17, 49
Tru1I TTAA 2 cut(s) 384, 426
Tru9I TTAA 2 cut(s) 384, 426
TscAI CASTG 2 cut(s) 299, 567
TspDTI ATGAA 1 cut(s) 473
TspGWI ACGGA 1 cut(s) 171
TspRI CASTG 2 cut(s) 299, 567
Tth111I GACNNNGTC 1 cut(s) 68
VpaK11BI GGWCC 1 cut(s) 518
XapI RAATTY 2 cut(s) 464, 797
XceI RCATGY 3 cut(s) 477, 549, 602
XhoI CTCGAG 1 cut(s) 370
XspI CTAG 2 cut(s) 255, 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.