MD07G1226900.v1.1

Autophagy-related protein 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010981
Physical Location & Seq
Forward (+)
30240314 .. 30245360
5047 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1226900.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGAAGTCCGACTCGGTTTTCTGATATGGGTTACGGTCATCTTAACGATTTTGGACCGAGTTAACTCGGTCTCCGCTAGCTGCCATTTCAGTGTCGTCGACGATGACAAGCTCTATAACTACACTTTGGACTCTCCCATCCCTTTCTTCCCTCACGGCGTCCAAAGCGAAGATGGGTTCTACAAGGTGGCAGCAAATGAGACTGTGCTCTGGTTTCAGCTTTGCAATGGAATGATTTTCAATCACGACCCACCTAGATGTGTTGATTGCTGGGATTGTGTGGGGGCATCATGCTGTGATATGGGTTCTAGTGCGCTTGTGTCAAACAACATAGGAGGTTACGATGTGTGCACTACCATTGGGCGTGCCTCAAGCATGGACATGAACATAATCGACAGGAAGAATCCTAACACTGGTGTCATTGTTAAGATGTCAAGTAGTGGCCTGAAGTTCAACTGTTCGCTCTCTGTGTCTGTAATATGTGATCCAAATAGAGTTCAGGGGCCACATTTACTAGAGAAAACTGGGAAATGCGACTACGCTACAGTATTAACGCATCCTTCTGGCTGTGCCAAGATTGAATACGTGCATGGAAGAGCGTGGGGCTGGTTTGGTACCTTAGGAATCATAATCTTATGCCTTTTTGGAGCTTATCTGCTGGTTGGGGCAGCTTATCGATATATAAAACTTGGAGTTCGTGGAATACACATTATACCAAACTTGGACTTTTGGACCACCTTACGTCAAAGTACCCAGAGTCTGTTTGCATCCTTACTGCACAAATTTAGGGGATCTTCTCATGGTCATCGAAGCACCTATTCTCCTGTCAACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
ATG27

Protein Analysis

279

Amino Acids

30.71

Weight (kDa)

7.54

Isoelectric Point (pI)

39.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG27 PF09451 21 - 266 2.3e-14 Autophagy-related protein 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010981)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40316 AT2G40316 AT2G40316
fragaria_vesca FvH4_7g25140 FvH4_7g25140
malus_domestica MD01G1158200.v1.1 MD07G1226900.v1.1
prunus_persica Prupe.2G256500_v2.0.a1
pyrus_communis pycom01g17490 pycom07g20550
rosa_chinensis RchiOBHm_Chr1g0371041
rosa_laevigata RLG00000026990
rosa_multiflora Rmu_sc0001018.1_g000028
rosa_roxburghii Rroxscaffold_4G00286040
rosa_rugosa Rorug01G0361000 Rorug01G0361100
rosa_samantha Rh1AG371300 Rh1CG348100 Rh1DG366000
rosa_wichuraiana Rw1G032660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 614
AccB1I GGYRCC 1 cut(s) 614
AccI GTMKAC 1 cut(s) 99
AciI CCGC 1 cut(s) 75
AclWI GGATC 2 cut(s) 479, 799
AcsI RAATTY 1 cut(s) 782
AcuI CTGAAG 1 cut(s) 467
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 2 cut(s) 616, 751
AfiI CCNNNNNNNGG 1 cut(s) 413
AgsI TTSAA 3 cut(s) 241, 454, 581
AluBI AGCT 5 cut(s) 81, 112, 220, 650, 671
AluI AGCT 5 cut(s) 81, 112, 220, 650, 671
Alw21I GWGCWC 2 cut(s) 210, 353
Alw26I GTCTC 2 cut(s) 76, 194
Alw44I GTGCAC 1 cut(s) 349
AlwI GGATC 2 cut(s) 479, 799
AlwNI CAGNNNCTG 1 cut(s) 760
AoxI GGCC 2 cut(s) 442, 503
ApaLI GTGCAC 1 cut(s) 349
ApeKI GCWGC 3 cut(s) 81, 191, 668
ApoI RAATTY 1 cut(s) 782
Asp718I GGTACC 1 cut(s) 614
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 3 cut(s) 55, 503, 732
AsuNHI GCTAGC 1 cut(s) 77
AvaII GGWCC 2 cut(s) 55, 732
AxyI CCTNAGG 1 cut(s) 619
BaeGI GKGCMC 1 cut(s) 353
BanI GGYRCC 1 cut(s) 614
Bbv12I GWGCWC 2 cut(s) 210, 353
BbvI GCAGC 3 cut(s) 68, 203, 680
BccI CCATC 2 cut(s) 146, 167
BceAI ACGGC 1 cut(s) 172
BcoDI GTCTC 2 cut(s) 76, 194
BfaI CTAG 4 cut(s) 78, 255, 309, 515
BfmI CTRYAG 1 cut(s) 543
BisI GCNGC 3 cut(s) 82, 192, 669
BlsI GCNGC 3 cut(s) 83, 193, 670
Bme18I GGWCC 2 cut(s) 55, 732
BmgT120I GGNCC 3 cut(s) 55, 503, 732
BmiI GGNNCC 2 cut(s) 504, 616
BmrI ACTGGG 1 cut(s) 534
BmsI GCATC 3 cut(s) 296, 565, 776
BmtI GCTAGC 1 cut(s) 81
BmuI ACTGGG 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 355
Bsa29I ATCGAT 1 cut(s) 676
BsaAI YACGTR 1 cut(s) 586
BsaHI GRCGYC 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 76
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 805, 835
Bsc4I CCNNNNNNNGG 1 cut(s) 413
Bse1I ACTGG 2 cut(s) 418, 529
Bse21I CCTNAGG 1 cut(s) 619
Bse3DI GCAATG 1 cut(s) 232
BseCI ATCGAT 1 cut(s) 676
BseGI GGATG 3 cut(s) 138, 556, 767
BseLI CCNNNNNNNGG 1 cut(s) 413
BseMI GCAATG 1 cut(s) 232
BseNI ACTGG 2 cut(s) 418, 529
BseSI GKGCMC 1 cut(s) 353
BseXI GCAGC 3 cut(s) 68, 203, 680
BseYI CCCAGC 1 cut(s) 270
BsgI GTGCAG 1 cut(s) 761
BshFI GGCC 2 cut(s) 444, 505
BshNI GGYRCC 1 cut(s) 614
BshVI ATCGAT 1 cut(s) 676
BsiHKAI GWGCWC 2 cut(s) 210, 353
BslI CCNNNNNNNGG 1 cut(s) 413
BsmAI GTCTC 2 cut(s) 76, 194
BsnI GGCC 2 cut(s) 444, 505
Bso31I GGTCTC 1 cut(s) 76
Bsp1286I GDGCHC 2 cut(s) 210, 353
Bsp143I GATC 2 cut(s) 484, 791
BspACI CCGC 1 cut(s) 75
BspANI GGCC 2 cut(s) 444, 505
BspDI ATCGAT 1 cut(s) 676
BspLI GGNNCC 2 cut(s) 504, 616
BspOI GCTAGC 1 cut(s) 81
BspPI GGATC 2 cut(s) 479, 799
BspQI GCTCTTC 1 cut(s) 589
BspT107I GGYRCC 1 cut(s) 614
BspTNI GGTCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 232
BsrI ACTGG 2 cut(s) 418, 529
BssMI GATC 2 cut(s) 484, 791
BssNI GRCGYC 1 cut(s) 159
Bst4CI ACNGT 4 cut(s) 37, 205, 458, 547
Bst6I CTCTTC 1 cut(s) 589
BstACI GRCGYC 1 cut(s) 159
BstBAI YACGTR 1 cut(s) 586
BstC8I GCNNGC 2 cut(s) 79, 366
BstDEI CTNAG 1 cut(s) 619
BstF5I GGATG 3 cut(s) 138, 556, 767
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 2 cut(s) 487, 794
BstMAI GTCTC 2 cut(s) 76, 194
BstMBI GATC 2 cut(s) 484, 791
BstMWI GCNNNNNNNGC 1 cut(s) 165
BstSFI CTRYAG 1 cut(s) 543
BstSLI GKGCMC 1 cut(s) 353
BstV1I GCAGC 3 cut(s) 68, 203, 680
BstX2I RGATCY 1 cut(s) 791
BstYI RGATCY 1 cut(s) 791
Bsu15I ATCGAT 1 cut(s) 676
Bsu36I CCTNAGG 1 cut(s) 619
BsuRI GGCC 2 cut(s) 444, 505
BsuTUI ATCGAT 1 cut(s) 676
BtsCI GGATG 3 cut(s) 138, 556, 767
BtsIMutI CAGTG 2 cut(s) 97, 411
Cac8I GCNNGC 2 cut(s) 79, 366
CaiI CAGNNNCTG 1 cut(s) 760
CfoI GCGC 1 cut(s) 316
Cfr13I GGNCC 3 cut(s) 55, 503, 732
ClaI ATCGAT 1 cut(s) 676
CseI GACGC 1 cut(s) 148
Csp6I GTAC 2 cut(s) 615, 750
CviAII CATG 5 cut(s) 291, 376, 382, 590, 800
CviJI RGCY 9 cut(s) 81, 112, 220, 444, 505, 567, 606, 650, 671
CviKI_1 RGCY 9 cut(s) 81, 112, 220, 444, 505, 567, 606, 650, 671
CviQI GTAC 2 cut(s) 615, 750
DdeI CTNAG 1 cut(s) 619
DpnI GATC 2 cut(s) 486, 793
DpnII GATC 2 cut(s) 484, 791
Eam1104I CTCTTC 1 cut(s) 589
EarI CTCTTC 1 cut(s) 589
Eco31I GGTCTC 1 cut(s) 76
Eco47I GGWCC 2 cut(s) 55, 732
Eco57I CTGAAG 1 cut(s) 467
Eco81I CCTNAGG 1 cut(s) 619
FaeI CATG 5 cut(s) 294, 379, 385, 593, 803
FatI CATG 5 cut(s) 290, 375, 381, 589, 799
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 3 cut(s) 82, 192, 669
FokI GGATG 3 cut(s) 125, 543, 754
Fsp4HI GCNGC 3 cut(s) 82, 192, 669
FspBI CTAG 4 cut(s) 78, 255, 309, 515
GlaI GCGC 1 cut(s) 315
GluI GCNGC 3 cut(s) 82, 192, 669
GsaI CCCAGC 1 cut(s) 274
HaeIII GGCC 2 cut(s) 444, 505
HgaI GACGC 1 cut(s) 148
HhaI GCGC 1 cut(s) 316
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 5 cut(s) 294, 379, 385, 593, 803
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HincII GTYRAC 3 cut(s) 64, 100, 829
HindII GTYRAC 3 cut(s) 64, 100, 829
HinfI GANTC 5 cut(s) 12, 131, 403, 624, 757
HpaI GTTAAC 1 cut(s) 64
Hpy166II GTNNAC 4 cut(s) 64, 100, 351, 829
Hpy188I TCNGA 2 cut(s) 11, 24
Hpy188III TCNNGA 1 cut(s) 245
Hpy8I GTNNAC 4 cut(s) 64, 100, 351, 829
Hpy99I CGWCG 2 cut(s) 101, 104
HpyAV CCTTC 1 cut(s) 570
HpyCH4III ACNGT 4 cut(s) 37, 205, 458, 547
HpyCH4IV ACGT 2 cut(s) 585, 742
HpyCH4V TGCA 5 cut(s) 225, 351, 589, 767, 778
HpyF10VI GCNNNNNNNGC 1 cut(s) 165
HpyF3I CTNAG 1 cut(s) 619
HpySE526I ACGT 2 cut(s) 585, 742
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 5 cut(s) 294, 379, 385, 593, 803
HspAI GCGC 1 cut(s) 314
KpnI GGTACC 1 cut(s) 618
KspAI GTTAAC 1 cut(s) 64
Kzo9I GATC 2 cut(s) 484, 791
LguI GCTCTTC 1 cut(s) 589
LmnI GCTCC 1 cut(s) 647
Lsp1109I GCAGC 3 cut(s) 68, 203, 680
LweI GCATC 3 cut(s) 296, 565, 776
MaeI CTAG 4 cut(s) 78, 255, 309, 515
MaeII ACGT 2 cut(s) 585, 742
MaeIII GTNAC 2 cut(s) 31, 338
MalI GATC 2 cut(s) 486, 793
MboI GATC 2 cut(s) 484, 791
MboII GAAGA 5 cut(s) 139, 182, 412, 606, 786
MflI RGATCY 1 cut(s) 791
MhlI GDGCHC 2 cut(s) 210, 353
MluCI AATT 1 cut(s) 782
MlyI GAGTC 3 cut(s) 6, 125, 766
MmeI TCCRAC 1 cut(s) 34
MnlI CCTC 3 cut(s) 162, 329, 379
MseI TTAA 4 cut(s) 44, 63, 426, 551
MslI CAYNNNNRTG 3 cut(s) 90, 256, 380
MwoI GCNNNNNNNGC 1 cut(s) 165
NdeII GATC 2 cut(s) 484, 791
NheI GCTAGC 1 cut(s) 77
NlaIII CATG 5 cut(s) 294, 379, 385, 593, 803
NlaIV GGNNCC 2 cut(s) 504, 616
PciSI GCTCTTC 1 cut(s) 589
PfeI GAWTC 2 cut(s) 403, 624
PkrI GCNGC 3 cut(s) 83, 193, 670
PleI GAGTC 3 cut(s) 6, 125, 765
PpsI GAGTC 3 cut(s) 6, 125, 765
Ppu21I YACGTR 1 cut(s) 586
PspFI CCCAGC 1 cut(s) 270
PspN4I GGNNCC 2 cut(s) 504, 616
PspPI GGNCC 3 cut(s) 55, 503, 732
PstNI CAGNNNCTG 1 cut(s) 760
PsuI RGATCY 1 cut(s) 791
RsaI GTAC 2 cut(s) 616, 751
RsaNI GTAC 2 cut(s) 615, 750
RseI CAYNNNNRTG 3 cut(s) 90, 256, 380
SalI GTCGAC 1 cut(s) 98
SapI GCTCTTC 1 cut(s) 589
SaqAI TTAA 4 cut(s) 44, 63, 426, 551
SatI GCNGC 3 cut(s) 82, 192, 669
Sau3AI GATC 2 cut(s) 484, 791
Sau96I GGNCC 3 cut(s) 55, 503, 732
SchI GAGTC 3 cut(s) 6, 125, 766
SduI GDGCHC 2 cut(s) 210, 353
SfaNI GCATC 3 cut(s) 296, 565, 776
SfcI CTRYAG 1 cut(s) 543
SgrDI CGTCGACG 1 cut(s) 98
SinI GGWCC 2 cut(s) 55, 732
SmiMI CAYNNNNRTG 3 cut(s) 90, 256, 380
SmlI CTYRAG 1 cut(s) 370
SmoI CTYRAG 1 cut(s) 370
Sse9I AATT 1 cut(s) 782
SsiI CCGC 1 cut(s) 75
SspMI CTAG 4 cut(s) 78, 255, 309, 515
TaaI ACNGT 4 cut(s) 37, 205, 458, 547
TaiI ACGT 2 cut(s) 588, 745
TaqI TCGA 4 cut(s) 99, 393, 676, 808
TaqII GACCGA 2 cut(s) 58, 72
TasI AATT 1 cut(s) 782
TfiI GAWTC 2 cut(s) 403, 624
Tru1I TTAA 4 cut(s) 44, 63, 426, 551
Tru9I TTAA 4 cut(s) 44, 63, 426, 551
TscAI CASTG 2 cut(s) 97, 418
TseI GCWGC 3 cut(s) 81, 191, 668
TspDTI ATGAA 1 cut(s) 398
TspRI CASTG 2 cut(s) 97, 418
VneI GTGCAC 1 cut(s) 349
VpaK11BI GGWCC 2 cut(s) 55, 732
XapI RAATTY 1 cut(s) 782
XcmI CCANNNNNNNNNTGG 1 cut(s) 170
XmiI GTMKAC 1 cut(s) 99
XspI CTAG 4 cut(s) 78, 255, 309, 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.