MD01G1158200.v1.1

Autophagy-related protein 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010981
Physical Location & Seq
Forward (+)
26501412 .. 26507213
5802 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1158200.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGAAGTCCGACTCGGTTTTCTGAAATGGGTTGCAGTCATCTTAACGATTCTGGACCGAGTTAACTCGGTCTCCGGTAGCTGCCATTTCAGTTTCGTCGACGATAACAAGCTCTACAACTACACTCTGGACTCTCCCATCCCTTTCTTCCCTCATGGCGTCCAAAGCGAAGATGGGTTCTACAAGGTGGCAGCAAATGAGACTGTGCTCTGGTTTCAGCTTTGCGATGGAATGATTTTCAATCACGACCCACCTAGATGTGTTGATTGCCGGGATTGTGGGGGTGCATCACGCTGTGGCATGGGTTGTAGTGCGCTTGTGGCAAACAACATAGAAGGTTACGATGTGTGCACTACCATTGGGCGTGCCTCAAGCATTGACATGAACATAATTGACAGGAAGAATCCTAACACTGGTGTCATTGTTAAGATGTCTAGTAGTGGCCTAAAGTTCAACTGTTCGCTGTCTGTGTCTGTGATTTGTGATCCCAATAGAGTTCAGGGGCCACATTCACTTGAGAAAACTGGGAAATGCGACTATGCTACAGTATTGACACATCCTTCTGGCTGTGCCAAGATTGAACACGTGCATGGGAGAGGGTGGGGCTGGCTTGGTACCTTAGGAATCATAATCTTATGCCTTTTTGGAGCTTATCTGCTGGTTGGGGCAGGTTATCGATATATCAAACTTGGAGTTCGTGGAATACACATTATACCAAACTTGGACTTTTGGACCGGCTTACCTCAAACTACACAGAGTTTGTTTGCATTCTTAATGCACAAATTTAGGGGCTCTTCTCATGGTCTTCGAAGCACCTATTCTCCTGTCGACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
ATG27

Protein Analysis

279

Amino Acids

30.56

Weight (kDa)

7.94

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG27 PF09451 24 - 266 2.4e-16 Autophagy-related protein 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010981)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40316 AT2G40316 AT2G40316
fragaria_vesca FvH4_7g25140 FvH4_7g25140
malus_domestica MD01G1158200.v1.1 MD07G1226900.v1.1
prunus_persica Prupe.2G256500_v2.0.a1
pyrus_communis pycom01g17490 pycom07g20550
rosa_chinensis RchiOBHm_Chr1g0371041
rosa_laevigata RLG00000026990
rosa_multiflora Rmu_sc0001018.1_g000028
rosa_roxburghii Rroxscaffold_4G00286040
rosa_rugosa Rorug01G0361000 Rorug01G0361100
rosa_samantha Rh1AG371300 Rh1CG348100 Rh1DG366000
rosa_wichuraiana Rw1G032660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 659
Acc65I GGTACC 1 cut(s) 614
AccB1I GGYRCC 1 cut(s) 614
AccI GTMKAC 2 cut(s) 99, 828
AclWI GGATC 1 cut(s) 479
AcsI RAATTY 1 cut(s) 782
AcvI CACGTG 1 cut(s) 586
AcyI GRCGYC 1 cut(s) 159
AdeI CACNNNGTG 1 cut(s) 296
AfaI GTAC 1 cut(s) 616
AfiI CCNNNNNNNGG 1 cut(s) 413
AflIII ACRYGT 1 cut(s) 583
AgsI TTSAA 3 cut(s) 241, 454, 581
AluBI AGCT 4 cut(s) 81, 112, 220, 650
AluI AGCT 4 cut(s) 81, 112, 220, 650
Alw21I GWGCWC 2 cut(s) 210, 353
Alw26I GTCTC 2 cut(s) 76, 194
Alw44I GTGCAC 1 cut(s) 349
AlwI GGATC 1 cut(s) 479
AoxI GGCC 2 cut(s) 442, 503
ApaLI GTGCAC 1 cut(s) 349
ApeKI GCWGC 2 cut(s) 81, 191
ApoI RAATTY 1 cut(s) 782
Asp718I GGTACC 1 cut(s) 614
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 3 cut(s) 55, 503, 732
AsuC2I CCSGG 1 cut(s) 272
AsuII TTCGAA 1 cut(s) 808
AvaII GGWCC 2 cut(s) 55, 732
AxyI CCTNAGG 1 cut(s) 619
BaeGI GKGCMC 1 cut(s) 353
BanI GGYRCC 1 cut(s) 614
BanII GRGCYC 1 cut(s) 794
BbrPI CACGTG 1 cut(s) 586
BbsI GAAGAC 1 cut(s) 797
Bbv12I GWGCWC 2 cut(s) 210, 353
BbvI GCAGC 2 cut(s) 68, 203
BccI CCATC 3 cut(s) 146, 167, 221
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 2 cut(s) 76, 194
BfaI CTAG 2 cut(s) 255, 435
BfmI CTRYAG 1 cut(s) 543
BfuAI ACCTGC 1 cut(s) 659
BisI GCNGC 2 cut(s) 82, 192
BlsI GCNGC 2 cut(s) 83, 193
Bme1390I CCNGG 1 cut(s) 272
Bme18I GGWCC 2 cut(s) 55, 732
BmgT120I GGNCC 3 cut(s) 55, 503, 732
BmiI GGNNCC 2 cut(s) 504, 616
BmrFI CCNGG 1 cut(s) 272
BmrI ACTGGG 1 cut(s) 534
BmsI GCATC 1 cut(s) 296
BmuI ACTGGG 1 cut(s) 534
BpiI GAAGAC 1 cut(s) 797
Bpu14I TTCGAA 1 cut(s) 808
BpuEI CTTGAG 2 cut(s) 355, 536
BpuMI CCSGG 1 cut(s) 272
Bsa29I ATCGAT 1 cut(s) 676
BsaAI YACGTR 1 cut(s) 586
BsaHI GRCGYC 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 76
BsaWI WCCGGW 1 cut(s) 74
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 805, 835
Bsc4I CCNNNNNNNGG 1 cut(s) 413
Bse118I RCCGGY 1 cut(s) 734
Bse1I ACTGG 2 cut(s) 418, 529
Bse21I CCTNAGG 1 cut(s) 619
BseCI ATCGAT 1 cut(s) 676
BseGI GGATG 2 cut(s) 138, 556
BseLI CCNNNNNNNGG 1 cut(s) 413
BseNI ACTGG 2 cut(s) 418, 529
BseSI GKGCMC 1 cut(s) 353
BseXI GCAGC 2 cut(s) 68, 203
BshFI GGCC 2 cut(s) 444, 505
BshNI GGYRCC 1 cut(s) 614
BshVI ATCGAT 1 cut(s) 676
BsiHKAI GWGCWC 2 cut(s) 210, 353
BsiSI CCGG 3 cut(s) 75, 271, 735
BslI CCNNNNNNNGG 1 cut(s) 413
BsmAI GTCTC 2 cut(s) 76, 194
BsmI GAATGC 1 cut(s) 767
BsnI GGCC 2 cut(s) 444, 505
Bso31I GGTCTC 1 cut(s) 76
Bsp119I TTCGAA 1 cut(s) 808
Bsp1286I GDGCHC 3 cut(s) 210, 353, 794
Bsp143I GATC 1 cut(s) 484
BspANI GGCC 2 cut(s) 444, 505
BspDI ATCGAT 1 cut(s) 676
BspLI GGNNCC 2 cut(s) 504, 616
BspMI ACCTGC 1 cut(s) 659
BspPI GGATC 1 cut(s) 479
BspQI GCTCTTC 1 cut(s) 799
BspT104I TTCGAA 1 cut(s) 808
BspT107I GGYRCC 1 cut(s) 614
BspTNI GGTCTC 1 cut(s) 76
BsrFI RCCGGY 1 cut(s) 734
BsrI ACTGG 2 cut(s) 418, 529
BssAI RCCGGY 1 cut(s) 734
BssMI GATC 1 cut(s) 484
BssNI GRCGYC 1 cut(s) 159
Bst4CI ACNGT 3 cut(s) 205, 458, 547
Bst6I CTCTTC 1 cut(s) 799
BstACI GRCGYC 1 cut(s) 159
BstBAI YACGTR 1 cut(s) 586
BstBI TTCGAA 1 cut(s) 808
BstC8I GCNNGC 2 cut(s) 366, 608
BstDEI CTNAG 1 cut(s) 619
BstF5I GGATG 2 cut(s) 138, 556
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 1 cut(s) 487
BstMAI GTCTC 2 cut(s) 76, 194
BstMBI GATC 1 cut(s) 484
BstMWI GCNNNNNNNGC 2 cut(s) 165, 320
BstSCI CCNGG 1 cut(s) 270
BstSFI CTRYAG 1 cut(s) 543
BstSLI GKGCMC 1 cut(s) 353
BstV1I GCAGC 2 cut(s) 68, 203
BstV2I GAAGAC 1 cut(s) 797
Bsu15I ATCGAT 1 cut(s) 676
Bsu36I CCTNAGG 1 cut(s) 619
BsuRI GGCC 2 cut(s) 444, 505
BsuTUI ATCGAT 1 cut(s) 676
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 2 cut(s) 138, 556
BtsIMutI CAGTG 1 cut(s) 411
BveI ACCTGC 1 cut(s) 659
Cac8I GCNNGC 2 cut(s) 366, 608
CfoI GCGC 1 cut(s) 316
Cfr10I RCCGGY 1 cut(s) 734
Cfr13I GGNCC 3 cut(s) 55, 503, 732
ClaI ATCGAT 1 cut(s) 676
CseI GACGC 1 cut(s) 148
Csp6I GTAC 1 cut(s) 615
CviAII CATG 5 cut(s) 155, 301, 382, 590, 800
CviQI GTAC 1 cut(s) 615
DdeI CTNAG 1 cut(s) 619
DpnI GATC 1 cut(s) 486
DpnII GATC 1 cut(s) 484
DraIII CACNNNGTG 1 cut(s) 296
Eam1104I CTCTTC 1 cut(s) 799
EarI CTCTTC 1 cut(s) 799
Eco24I GRGCYC 1 cut(s) 794
Eco31I GGTCTC 1 cut(s) 76
Eco47I GGWCC 2 cut(s) 55, 732
Eco72I CACGTG 1 cut(s) 586
Eco81I CCTNAGG 1 cut(s) 619
EcoT38I GRGCYC 1 cut(s) 794
FaeI CATG 5 cut(s) 158, 304, 385, 593, 803
FatI CATG 5 cut(s) 154, 300, 381, 589, 799
FblI GTMKAC 2 cut(s) 99, 828
Fnu4HI GCNGC 2 cut(s) 82, 192
FokI GGATG 2 cut(s) 125, 543
FriOI GRGCYC 1 cut(s) 794
Fsp4HI GCNGC 2 cut(s) 82, 192
FspBI CTAG 2 cut(s) 255, 435
GlaI GCGC 1 cut(s) 315
GluI GCNGC 2 cut(s) 82, 192
HaeIII GGCC 2 cut(s) 444, 505
HapII CCGG 3 cut(s) 75, 271, 735
HgaI GACGC 1 cut(s) 148
HhaI GCGC 1 cut(s) 316
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 5 cut(s) 158, 304, 385, 593, 803
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HincII GTYRAC 3 cut(s) 64, 100, 829
HindII GTYRAC 3 cut(s) 64, 100, 829
HinfI GANTC 5 cut(s) 12, 49, 131, 403, 624
HpaI GTTAAC 1 cut(s) 64
HpaII CCGG 3 cut(s) 75, 271, 735
Hpy166II GTNNAC 4 cut(s) 64, 100, 351, 829
Hpy188I TCNGA 2 cut(s) 11, 24
Hpy188III TCNNGA 3 cut(s) 53, 128, 245
Hpy8I GTNNAC 4 cut(s) 64, 100, 351, 829
Hpy99I CGWCG 2 cut(s) 101, 104
HpyAV CCTTC 2 cut(s) 329, 570
HpyCH4III ACNGT 3 cut(s) 205, 458, 547
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 6 cut(s) 35, 287, 351, 589, 767, 778
HpyF10VI GCNNNNNNNGC 2 cut(s) 165, 320
HpyF3I CTNAG 1 cut(s) 619
HpySE526I ACGT 1 cut(s) 585
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 5 cut(s) 158, 304, 385, 593, 803
HspAI GCGC 1 cut(s) 314
KpnI GGTACC 1 cut(s) 618
KspAI GTTAAC 1 cut(s) 64
Kzo9I GATC 1 cut(s) 484
LguI GCTCTTC 1 cut(s) 799
LmnI GCTCC 1 cut(s) 647
Lsp1109I GCAGC 2 cut(s) 68, 203
LweI GCATC 1 cut(s) 296
MaeI CTAG 2 cut(s) 255, 435
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 1 cut(s) 338
MalI GATC 1 cut(s) 486
MboI GATC 1 cut(s) 484
MboII GAAGA 5 cut(s) 139, 182, 412, 786, 797
MhlI GDGCHC 3 cut(s) 210, 353, 794
MluCI AATT 2 cut(s) 390, 782
MlyI GAGTC 2 cut(s) 6, 125
MmeI TCCRAC 1 cut(s) 34
MnlI CCTC 4 cut(s) 162, 379, 590, 753
MseI TTAA 4 cut(s) 44, 63, 426, 773
MslI CAYNNNNRTG 3 cut(s) 256, 380, 588
MspI CCGG 3 cut(s) 75, 271, 735
MspR9I CCNGG 1 cut(s) 272
Mva1269I GAATGC 1 cut(s) 767
MwoI GCNNNNNNNGC 2 cut(s) 165, 320
NciI CCSGG 1 cut(s) 272
NdeII GATC 1 cut(s) 484
NlaIII CATG 5 cut(s) 158, 304, 385, 593, 803
NlaIV GGNNCC 2 cut(s) 504, 616
NspV TTCGAA 1 cut(s) 808
PciSI GCTCTTC 1 cut(s) 799
PctI GAATGC 1 cut(s) 767
PfeI GAWTC 3 cut(s) 49, 403, 624
PkrI GCNGC 2 cut(s) 83, 193
PleI GAGTC 2 cut(s) 6, 125
PmaCI CACGTG 1 cut(s) 586
PmlI CACGTG 1 cut(s) 586
PpsI GAGTC 2 cut(s) 6, 125
Ppu21I YACGTR 1 cut(s) 586
PspCI CACGTG 1 cut(s) 586
PspN4I GGNNCC 2 cut(s) 504, 616
PspPI GGNCC 3 cut(s) 55, 503, 732
RsaI GTAC 1 cut(s) 616
RsaNI GTAC 1 cut(s) 615
RseI CAYNNNNRTG 3 cut(s) 256, 380, 588
SalI GTCGAC 2 cut(s) 98, 827
SapI GCTCTTC 1 cut(s) 799
SaqAI TTAA 4 cut(s) 44, 63, 426, 773
SatI GCNGC 2 cut(s) 82, 192
Sau3AI GATC 1 cut(s) 484
Sau96I GGNCC 3 cut(s) 55, 503, 732
SchI GAGTC 2 cut(s) 6, 125
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 3 cut(s) 210, 353, 794
SfaNI GCATC 1 cut(s) 296
SfcI CTRYAG 1 cut(s) 543
SfuI TTCGAA 1 cut(s) 808
SgrDI CGTCGACG 1 cut(s) 98
SinI GGWCC 2 cut(s) 55, 732
SmiMI CAYNNNNRTG 3 cut(s) 256, 380, 588
SmlI CTYRAG 2 cut(s) 370, 515
SmoI CTYRAG 2 cut(s) 370, 515
Sse9I AATT 2 cut(s) 390, 782
SspMI CTAG 2 cut(s) 255, 435
StyD4I CCNGG 1 cut(s) 270
TaaI ACNGT 3 cut(s) 205, 458, 547
TaiI ACGT 1 cut(s) 588
TaqI TCGA 4 cut(s) 99, 676, 808, 828
TaqII GACCGA 2 cut(s) 58, 72
TasI AATT 2 cut(s) 390, 782
TfiI GAWTC 3 cut(s) 49, 403, 624
Tru1I TTAA 4 cut(s) 44, 63, 426, 773
Tru9I TTAA 4 cut(s) 44, 63, 426, 773
TscAI CASTG 1 cut(s) 418
TseI GCWGC 2 cut(s) 81, 191
TspDTI ATGAA 1 cut(s) 398
TspRI CASTG 1 cut(s) 418
VneI GTGCAC 1 cut(s) 349
VpaK11BI GGWCC 2 cut(s) 55, 732
XapI RAATTY 1 cut(s) 782
XcmI CCANNNNNNNNNTGG 1 cut(s) 170
XmiI GTMKAC 2 cut(s) 99, 828
XspI CTAG 2 cut(s) 255, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.