FvH4_7g25140

Autophagy-related protein 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0010981
Physical Location & Seq
Forward (+)
19239210 .. 19243407
4198 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25140.t1

Sequence Viewer

Length: 606 bp
ATGATTTTCAATCATGACCCACCTGGATGTGTTGATTGCTTGGACTGTGGCGGTCCATCACACTGTGGCATGGGTTGTAGTGCTCTTGTAGCGAAAAACATAGGAGGTTACGATGTTTGCACTACAGTTGGGCGTGCCGCGAGCATGGACATTAACATAATTGATAAGAACAATCCTCACATTGGTGTCATTGTTAAGATGTCAAGTAATGGCCTAAAGGGAAATTGTTCACTCTCTGTGTCTGTAATGTGTGATCCTAATGGAGTTCAGGAACCATATTTGTTGGAGAAAACAGGGAAATGTGACTATGCTACAATGCTAAAGCATCCTTCTGGCTGTGCCAAGATTGTACATGTTTATGGCAAAGGATGGGGCTGGTTTGTAACCTTCCTAATCATCATCTTGTGCCTTTTTGGAGTGTATCTGTTGGCTGGTGCAGGTTATCGATATTTCAGTCTTGGCTTTCATGGAATAGATATTTTACCAAACTTGGACTTTTGGACCGGTTTACCTCAGCAAGCACAGAGTGTGTCTGCTTCTTTATTGCACAAATTTAGGGGATCTTCTCAAGTTCATCGAAGCTCCTATTCTCCTGTCAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
ATG27

Protein Analysis

202

Amino Acids

21.64

Weight (kDa)

8.0

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG27 PF09451 47 - 190 2.3e-15 Autophagy-related protein 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010981)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40316 AT2G40316 AT2G40316
fragaria_vesca FvH4_7g25140 FvH4_7g25140
malus_domestica MD01G1158200.v1.1 MD07G1226900.v1.1
prunus_persica Prupe.2G256500_v2.0.a1
pyrus_communis pycom01g17490 pycom07g20550
rosa_chinensis RchiOBHm_Chr1g0371041
rosa_laevigata RLG00000026990
rosa_multiflora Rmu_sc0001018.1_g000028
rosa_roxburghii Rroxscaffold_4G00286040
rosa_rugosa Rorug01G0361000 Rorug01G0361100
rosa_samantha Rh1AG371300 Rh1CG348100 Rh1DG366000
rosa_wichuraiana Rw1G032660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 428
AccII CGCG 1 cut(s) 140
AciI CCGC 2 cut(s) 51, 138
AclWI GGATC 2 cut(s) 248, 568
AcsI RAATTY 1 cut(s) 551
AdeI CACNNNGTG 2 cut(s) 65, 527
AfaI GTAC 1 cut(s) 351
AfiI CCNNNNNNNGG 1 cut(s) 182
AflIII ACRYGT 1 cut(s) 352
AgeI ACCGGT 1 cut(s) 503
AgsI TTSAA 1 cut(s) 10
AjnI CCWGG 1 cut(s) 22
AleI CACNNNNGTG 1 cut(s) 183
AluBI AGCT 1 cut(s) 582
AluI AGCT 1 cut(s) 582
Alw21I GWGCWC 1 cut(s) 85
AlwI GGATC 2 cut(s) 248, 568
AoxI GGCC 1 cut(s) 211
ApoI RAATTY 1 cut(s) 551
AsiGI ACCGGT 1 cut(s) 503
Asp700I GAANNNNTTC 1 cut(s) 226
AspS9I GGNCC 2 cut(s) 53, 501
AvaII GGWCC 2 cut(s) 53, 501
Bbv12I GWGCWC 1 cut(s) 85
BbvCI CCTCAGC 1 cut(s) 513
BccI CCATC 2 cut(s) 64, 363
BciT130I CCWGG 1 cut(s) 24
BfmI CTRYAG 1 cut(s) 123
BfuAI ACCTGC 1 cut(s) 428
BisI GCNGC 1 cut(s) 138
BlsI GCNGC 1 cut(s) 139
Bme1390I CCNGG 1 cut(s) 24
Bme18I GGWCC 2 cut(s) 53, 501
BmgT120I GGNCC 2 cut(s) 53, 501
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 1 cut(s) 24
BmsI GCATC 1 cut(s) 334
Bpu10I CCTNAGC 1 cut(s) 513
BpuEI CTTGAG 1 cut(s) 552
Bsa29I ATCGAT 1 cut(s) 445
BsaWI WCCGGW 1 cut(s) 503
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse118I RCCGGY 1 cut(s) 503
BseBI CCWGG 1 cut(s) 24
BseCI ATCGAT 1 cut(s) 445
BseGI GGATG 3 cut(s) 32, 325, 374
BseLI CCNNNNNNNGG 1 cut(s) 182
BseMII CTCAG 1 cut(s) 527
BsgI GTGCAG 1 cut(s) 456
Bsh1236I CGCG 1 cut(s) 140
BshFI GGCC 1 cut(s) 213
BshTI ACCGGT 1 cut(s) 503
BshVI ATCGAT 1 cut(s) 445
BsiHKAI GWGCWC 1 cut(s) 85
BsiSI CCGG 1 cut(s) 504
BslI CCNNNNNNNGG 1 cut(s) 182
BsnI GGCC 1 cut(s) 213
Bsp1286I GDGCHC 1 cut(s) 85
Bsp1407I TGTACA 1 cut(s) 349
Bsp143I GATC 2 cut(s) 253, 560
BspACI CCGC 2 cut(s) 51, 138
BspANI GGCC 1 cut(s) 213
BspCNI CTCAG 1 cut(s) 526
BspDI ATCGAT 1 cut(s) 445
BspFNI CGCG 1 cut(s) 140
BspHI TCATGA 1 cut(s) 13
BspLI GGNNCC 1 cut(s) 273
BspMI ACCTGC 1 cut(s) 428
BspPI GGATC 2 cut(s) 248, 568
BsrFI RCCGGY 1 cut(s) 503
BsrGI TGTACA 1 cut(s) 349
BssAI RCCGGY 1 cut(s) 503
BssMI GATC 2 cut(s) 253, 560
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 3 cut(s) 47, 65, 127
BstAUI TGTACA 1 cut(s) 349
BstC8I GCNNGC 3 cut(s) 135, 142, 519
BstDEI CTNAG 1 cut(s) 513
BstF5I GGATG 3 cut(s) 32, 325, 374
BstFNI CGCG 1 cut(s) 140
BstKTI GATC 2 cut(s) 256, 563
BstMBI GATC 2 cut(s) 253, 560
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstNI CCWGG 1 cut(s) 24
BstNSI RCATGY 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 22
BstSFI CTRYAG 1 cut(s) 123
BstUI CGCG 1 cut(s) 140
BstX2I RGATCY 1 cut(s) 560
BstYI RGATCY 1 cut(s) 560
Bsu15I ATCGAT 1 cut(s) 445
BsuRI GGCC 1 cut(s) 213
BsuTUI ATCGAT 1 cut(s) 445
BtsCI GGATG 3 cut(s) 32, 325, 374
BtsIMutI CAGTG 1 cut(s) 61
BveI ACCTGC 1 cut(s) 428
Cac8I GCNNGC 3 cut(s) 135, 142, 519
CciI TCATGA 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 503
Cfr13I GGNCC 2 cut(s) 53, 501
ClaI ATCGAT 1 cut(s) 445
Csp6I GTAC 1 cut(s) 350
CspAI ACCGGT 1 cut(s) 503
CviAII CATG 5 cut(s) 14, 70, 145, 353, 467
CviJI RGCY 6 cut(s) 213, 336, 375, 431, 462, 582
CviKI_1 RGCY 6 cut(s) 213, 336, 375, 431, 462, 582
CviQI GTAC 1 cut(s) 350
DdeI CTNAG 1 cut(s) 513
DpnI GATC 2 cut(s) 255, 562
DpnII GATC 2 cut(s) 253, 560
DraIII CACNNNGTG 2 cut(s) 65, 527
Eco47I GGWCC 2 cut(s) 53, 501
EcoRII CCWGG 1 cut(s) 22
FaeI CATG 5 cut(s) 17, 73, 148, 356, 470
FatI CATG 5 cut(s) 13, 69, 144, 352, 466
Fnu4HI GCNGC 1 cut(s) 138
FokI GGATG 3 cut(s) 39, 312, 381
Fsp4HI GCNGC 1 cut(s) 138
GluI GCNGC 1 cut(s) 138
HaeIII GGCC 1 cut(s) 213
HapII CCGG 1 cut(s) 504
Hin1II CATG 5 cut(s) 17, 73, 148, 356, 470
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HpaII CCGG 1 cut(s) 504
Hpy166II GTNNAC 3 cut(s) 230, 509, 598
Hpy188I TCNGA 1 cut(s) 605
Hpy188III TCNNGA 2 cut(s) 14, 269
Hpy8I GTNNAC 3 cut(s) 230, 509, 598
HpyAV CCTTC 2 cut(s) 339, 397
HpyCH4III ACNGT 3 cut(s) 47, 65, 127
HpyCH4V TGCA 3 cut(s) 120, 437, 547
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
HpyF3I CTNAG 1 cut(s) 513
Hsp92II CATG 5 cut(s) 17, 73, 148, 356, 470
Kzo9I GATC 2 cut(s) 253, 560
LmnI GCTCC 1 cut(s) 587
LpnPI CCDG 9 cut(s) 9, 36, 254, 279, 318, 361, 417, 423, 517
LweI GCATC 1 cut(s) 334
MaeIII GTNAC 3 cut(s) 107, 302, 382
MalI GATC 2 cut(s) 255, 562
MboI GATC 2 cut(s) 253, 560
MboII GAAGA 1 cut(s) 555
MflI RGATCY 1 cut(s) 560
MhlI GDGCHC 1 cut(s) 85
MluCI AATT 3 cut(s) 159, 223, 551
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 3 cut(s) 98, 186, 522
MroXI GAANNNNTTC 1 cut(s) 226
MseI TTAA 2 cut(s) 153, 195
MslI CAYNNNNRTG 3 cut(s) 25, 183, 357
MspI CCGG 1 cut(s) 504
MspR9I CCNGG 1 cut(s) 24
MvaI CCWGG 1 cut(s) 24
MvnI CGCG 1 cut(s) 140
MwoI GCNNNNNNNGC 1 cut(s) 89
NdeII GATC 2 cut(s) 253, 560
NlaIII CATG 5 cut(s) 17, 73, 148, 356, 470
NlaIV GGNNCC 1 cut(s) 273
NmuCI GTSAC 1 cut(s) 302
NspI RCATGY 1 cut(s) 356
OliI CACNNNNGTG 1 cut(s) 183
PagI TCATGA 1 cut(s) 13
PciI ACATGT 1 cut(s) 352
PdmI GAANNNNTTC 1 cut(s) 226
PinAI ACCGGT 1 cut(s) 503
PkrI GCNGC 1 cut(s) 139
PscI ACATGT 1 cut(s) 352
Psp6I CCWGG 1 cut(s) 22
PspGI CCWGG 1 cut(s) 22
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 2 cut(s) 53, 501
PsuI RGATCY 1 cut(s) 560
RsaI GTAC 1 cut(s) 351
RsaNI GTAC 1 cut(s) 350
RseI CAYNNNNRTG 3 cut(s) 25, 183, 357
SaqAI TTAA 2 cut(s) 153, 195
SatI GCNGC 1 cut(s) 138
Sau3AI GATC 2 cut(s) 253, 560
Sau96I GGNCC 2 cut(s) 53, 501
ScrFI CCNGG 1 cut(s) 24
SduI GDGCHC 1 cut(s) 85
SetI ASST 6 cut(s) 25, 109, 389, 442, 514, 584
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 1 cut(s) 123
SinI GGWCC 2 cut(s) 53, 501
SmiMI CAYNNNNRTG 3 cut(s) 25, 183, 357
SmlI CTYRAG 1 cut(s) 567
SmoI CTYRAG 1 cut(s) 567
Sse9I AATT 3 cut(s) 159, 223, 551
SsiI CCGC 2 cut(s) 51, 138
StyD4I CCNGG 1 cut(s) 22
TaaI ACNGT 3 cut(s) 47, 65, 127
TaqI TCGA 2 cut(s) 445, 577
TasI AATT 3 cut(s) 159, 223, 551
TatI WGTACW 1 cut(s) 349
TauI GCSGC 1 cut(s) 140
Tru1I TTAA 2 cut(s) 153, 195
Tru9I TTAA 2 cut(s) 153, 195
TscAI CASTG 1 cut(s) 68
TseFI GTSAC 1 cut(s) 302
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 2 cut(s) 455, 563
TspRI CASTG 1 cut(s) 68
VpaK11BI GGWCC 2 cut(s) 53, 501
XapI RAATTY 1 cut(s) 551
XceI RCATGY 1 cut(s) 356
XmnI GAANNNNTTC 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.