Prupe.2G256500_v2.0.a1

Autophagy-related protein 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0010981
Physical Location & Seq
Forward (+)
26838444 .. 26842784
4341 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G256500.1

Sequence Viewer

Length: 840 bp
ATGGTGGTCGGAGGCGGTTTTATGAAATGGGTTTTAGTTATTTTAGCGATTCTGGAACGAGTTAACTCGGTCTCTGCTAACTGTCATTTCAGTGTCGTTGATGATAACAAGCTCTACGACTACAGCTTGGGCTCTCCCATCCCCTTATTCCCTCATGGCGTCCAAAGCGAAGATGGATTCTACAAGGTGGCAGCAAATGAGACTGTGCTCTGGTTTCAGCTTTGCGATGGAATGATTTTCAATCACGACCCACCTAGATGCGTCGATTGCTGGGATTGTGGGGGGCCATCACGCTGTGGCATGGGTTGTAGTGCGCTTGTGGCAAATAACATAGGAGGTTATGATGTGTGCACTACCATTGGGCGTGCCTCGAGCATTGACATTAACATAATTGACAAGAAGAATCCTAACAGTGGTGTCATTGTTAAGATGTCAAATAGTGGCCTAAAGCTCAACTGTTCGCTCTCTGTGTCTGTAATTTGTGATCCAAATAGAGTTCAGGGGCCACATTCACTGGAGAAAACTGGGAAATGTGACTACGCTACAGTGTTAACGCATCCTTCTGGTTGTGCCAAGGTCGTACATGTTCATGGACACTGGTGGGGCTGGTTTGGCACCTTAGGAACCATAGTCTTATGCCTTTTTGGAGCATATCTGCTGGCTGGGGCAGTTTATCAATATTTCAGTCTTGGAGTTCGTGGAATACACGTTATTCCAAACTTGGACTTTTGGACCAGCTTACCTCAGAGAACACAGAGTCTGTTTGCATCTTTATTGCGCAAATTTAGGGGATCTTCTTCTCAAGGTCATCGAAGCTCGTATTCTCCTGTCAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
ATG27

Protein Analysis

280

Amino Acids

30.37

Weight (kDa)

8.21

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010981)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40316 AT2G40316 AT2G40316
fragaria_vesca FvH4_7g25140 FvH4_7g25140
malus_domestica MD01G1158200.v1.1 MD07G1226900.v1.1
prunus_persica Prupe.2G256500_v2.0.a1
pyrus_communis pycom01g17490 pycom07g20550
rosa_chinensis RchiOBHm_Chr1g0371041
rosa_laevigata RLG00000026990
rosa_multiflora Rmu_sc0001018.1_g000028
rosa_roxburghii Rroxscaffold_4G00286040
rosa_rugosa Rorug01G0361000 Rorug01G0361100
rosa_samantha Rh1AG371300 Rh1CG348100 Rh1DG366000
rosa_wichuraiana Rw1G032660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 779
AccB1I GGYRCC 1 cut(s) 614
AciI CCGC 1 cut(s) 15
AclWI GGATC 2 cut(s) 479, 799
AcsI RAATTY 1 cut(s) 782
AcyI GRCGYC 1 cut(s) 159
AdeI CACNNNGTG 1 cut(s) 296
AfaI GTAC 1 cut(s) 582
AfiI CCNNNNNNNGG 1 cut(s) 413
AflIII ACRYGT 2 cut(s) 583, 706
AgsI TTSAA 1 cut(s) 241
AluBI AGCT 6 cut(s) 112, 126, 220, 451, 738, 816
AluI AGCT 6 cut(s) 112, 126, 220, 451, 738, 816
Alw21I GWGCWC 2 cut(s) 210, 353
Alw26I GTCTC 2 cut(s) 76, 194
Alw44I GTGCAC 1 cut(s) 349
AlwI GGATC 2 cut(s) 479, 799
AlwNI CAGNNNCTG 1 cut(s) 760
Ama87I CYCGRG 1 cut(s) 370
AoxI GGCC 3 cut(s) 284, 442, 503
ApaLI GTGCAC 1 cut(s) 349
ApeKI GCWGC 1 cut(s) 191
ApoI RAATTY 1 cut(s) 782
AspLEI GCGC 2 cut(s) 316, 780
AspS9I GGNCC 3 cut(s) 284, 503, 732
AvaI CYCGRG 1 cut(s) 370
AvaII GGWCC 1 cut(s) 732
AxyI CCTNAGG 1 cut(s) 619
BaeGI GKGCMC 1 cut(s) 353
BanI GGYRCC 1 cut(s) 614
BanII GRGCYC 1 cut(s) 134
Bbv12I GWGCWC 2 cut(s) 210, 353
BbvI GCAGC 1 cut(s) 203
BccI CCATC 4 cut(s) 146, 167, 221, 295
BcoDI GTCTC 2 cut(s) 76, 194
BfaI CTAG 1 cut(s) 255
BfmI CTRYAG 2 cut(s) 121, 543
BisI GCNGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 193
Bme18I GGWCC 1 cut(s) 732
BmeT110I CYCGRG 1 cut(s) 370
BmgT120I GGNCC 3 cut(s) 284, 503, 732
BmiI GGNNCC 4 cut(s) 285, 504, 616, 625
BmrI ACTGGG 1 cut(s) 534
BmsI GCATC 3 cut(s) 248, 565, 776
BmuI ACTGGG 1 cut(s) 534
BpmI CTGGAG 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 786
BsaHI GRCGYC 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 1 cut(s) 573
Bsc4I CCNNNNNNNGG 1 cut(s) 413
Bse1I ACTGG 3 cut(s) 519, 529, 602
Bse21I CCTNAGG 1 cut(s) 619
BseDI CCNNGG 1 cut(s) 573
BseGI GGATG 2 cut(s) 138, 556
BseLI CCNNNNNNNGG 1 cut(s) 413
BseMII CTCAG 1 cut(s) 758
BseNI ACTGG 3 cut(s) 519, 529, 602
BseSI GKGCMC 1 cut(s) 353
BseXI GCAGC 1 cut(s) 203
BseYI CCCAGC 2 cut(s) 270, 662
BshFI GGCC 3 cut(s) 286, 444, 505
BshNI GGYRCC 1 cut(s) 614
BsiHKAI GWGCWC 2 cut(s) 210, 353
BsiHKCI CYCGRG 1 cut(s) 370
BslI CCNNNNNNNGG 1 cut(s) 413
BsmAI GTCTC 2 cut(s) 76, 194
BsnI GGCC 3 cut(s) 286, 444, 505
Bso31I GGTCTC 1 cut(s) 76
BsoBI CYCGRG 1 cut(s) 370
Bsp1286I GDGCHC 3 cut(s) 134, 210, 353
Bsp143I GATC 2 cut(s) 484, 791
BspACI CCGC 1 cut(s) 15
BspANI GGCC 3 cut(s) 286, 444, 505
BspCNI CTCAG 1 cut(s) 757
BspLI GGNNCC 4 cut(s) 285, 504, 616, 625
BspPI GGATC 2 cut(s) 479, 799
BspT107I GGYRCC 1 cut(s) 614
BspTNI GGTCTC 1 cut(s) 76
BsrI ACTGG 3 cut(s) 519, 529, 602
BssECI CCNNGG 1 cut(s) 573
BssMI GATC 2 cut(s) 484, 791
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 573
Bst4CI ACNGT 5 cut(s) 83, 205, 413, 458, 547
BstACI GRCGYC 1 cut(s) 159
BstC8I GCNNGC 2 cut(s) 366, 660
BstDEI CTNAG 2 cut(s) 619, 744
BstF5I GGATG 2 cut(s) 138, 556
BstHHI GCGC 2 cut(s) 316, 780
BstKTI GATC 2 cut(s) 487, 794
BstMAI GTCTC 2 cut(s) 76, 194
BstMBI GATC 2 cut(s) 484, 791
BstMWI GCNNNNNNNGC 4 cut(s) 165, 267, 320, 612
BstNSI RCATGY 1 cut(s) 587
BstSFI CTRYAG 2 cut(s) 121, 543
BstSLI GKGCMC 1 cut(s) 353
BstV1I GCAGC 1 cut(s) 203
BstX2I RGATCY 1 cut(s) 791
BstYI RGATCY 1 cut(s) 791
Bsu36I CCTNAGG 1 cut(s) 619
BsuRI GGCC 3 cut(s) 286, 444, 505
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 2 cut(s) 138, 556
BtsIMutI CAGTG 5 cut(s) 97, 418, 512, 552, 595
Cac8I GCNNGC 2 cut(s) 366, 660
CaiI CAGNNNCTG 1 cut(s) 760
CfoI GCGC 2 cut(s) 316, 780
Cfr13I GGNCC 3 cut(s) 284, 503, 732
CseI GACGC 2 cut(s) 148, 250
Csp6I GTAC 1 cut(s) 581
CviAII CATG 4 cut(s) 155, 301, 584, 590
CviQI GTAC 1 cut(s) 581
DdeI CTNAG 2 cut(s) 619, 744
DpnI GATC 2 cut(s) 486, 793
DpnII GATC 2 cut(s) 484, 791
DraIII CACNNNGTG 1 cut(s) 296
Eco130I CCWWGG 1 cut(s) 573
Eco24I GRGCYC 1 cut(s) 134
Eco31I GGTCTC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 732
Eco81I CCTNAGG 1 cut(s) 619
Eco88I CYCGRG 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 573
EcoT38I GRGCYC 1 cut(s) 134
ErhI CCWWGG 1 cut(s) 573
FaeI CATG 4 cut(s) 158, 304, 587, 593
FatI CATG 4 cut(s) 154, 300, 583, 589
Fnu4HI GCNGC 1 cut(s) 192
FokI GGATG 2 cut(s) 125, 543
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 1 cut(s) 192
FspBI CTAG 1 cut(s) 255
FspI TGCGCA 1 cut(s) 779
GlaI GCGC 2 cut(s) 315, 779
GluI GCNGC 1 cut(s) 192
GsaI CCCAGC 2 cut(s) 274, 666
GsuI CTGGAG 1 cut(s) 536
HaeIII GGCC 3 cut(s) 286, 444, 505
HgaI GACGC 2 cut(s) 148, 250
HhaI GCGC 2 cut(s) 316, 780
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 4 cut(s) 158, 304, 587, 593
Hin6I GCGC 2 cut(s) 314, 778
HinP1I GCGC 2 cut(s) 314, 778
HincII GTYRAC 3 cut(s) 64, 552, 832
HindII GTYRAC 3 cut(s) 64, 552, 832
HinfI GANTC 4 cut(s) 49, 177, 403, 757
HpaI GTTAAC 2 cut(s) 64, 552
Hpy166II GTNNAC 4 cut(s) 64, 351, 552, 832
Hpy188I TCNGA 3 cut(s) 11, 747, 839
Hpy188III TCNNGA 2 cut(s) 53, 245
Hpy8I GTNNAC 4 cut(s) 64, 351, 552, 832
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 1 cut(s) 570
HpyCH4III ACNGT 5 cut(s) 83, 205, 413, 458, 547
HpyCH4IV ACGT 1 cut(s) 708
HpyCH4V TGCA 2 cut(s) 351, 767
HpyF10VI GCNNNNNNNGC 4 cut(s) 165, 267, 320, 612
HpyF3I CTNAG 2 cut(s) 619, 744
HpySE526I ACGT 1 cut(s) 708
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 4 cut(s) 158, 304, 587, 593
HspAI GCGC 2 cut(s) 314, 778
KspAI GTTAAC 2 cut(s) 64, 552
Kzo9I GATC 2 cut(s) 484, 791
LmnI GCTCC 1 cut(s) 647
Lsp1109I GCAGC 1 cut(s) 203
LweI GCATC 3 cut(s) 248, 565, 776
MaeI CTAG 1 cut(s) 255
MaeII ACGT 1 cut(s) 708
MaeIII GTNAC 1 cut(s) 533
MalI GATC 2 cut(s) 486, 793
MboI GATC 2 cut(s) 484, 791
MboII GAAGA 4 cut(s) 182, 412, 786, 789
MflI RGATCY 1 cut(s) 791
MhlI GDGCHC 3 cut(s) 134, 210, 353
MluCI AATT 3 cut(s) 390, 477, 782
MlyI GAGTC 1 cut(s) 766
MnlI CCTC 5 cut(s) 5, 162, 329, 379, 753
MseI TTAA 4 cut(s) 63, 384, 426, 551
MslI CAYNNNNRTG 3 cut(s) 90, 256, 588
MwoI GCNNNNNNNGC 4 cut(s) 165, 267, 320, 612
NdeII GATC 2 cut(s) 484, 791
NlaIII CATG 4 cut(s) 158, 304, 587, 593
NlaIV GGNNCC 4 cut(s) 285, 504, 616, 625
NmuCI GTSAC 1 cut(s) 533
NsbI TGCGCA 1 cut(s) 779
NspI RCATGY 1 cut(s) 587
PaeR7I CTCGAG 1 cut(s) 370
PciI ACATGT 1 cut(s) 583
PfeI GAWTC 3 cut(s) 49, 177, 403
PkrI GCNGC 1 cut(s) 193
PleI GAGTC 1 cut(s) 765
PpsI GAGTC 1 cut(s) 765
PscI ACATGT 1 cut(s) 583
PspFI CCCAGC 2 cut(s) 270, 662
PspN4I GGNNCC 4 cut(s) 285, 504, 616, 625
PspPI GGNCC 3 cut(s) 284, 503, 732
PspXI VCTCGAGB 1 cut(s) 370
PstNI CAGNNNCTG 1 cut(s) 760
PsuI RGATCY 1 cut(s) 791
RsaI GTAC 1 cut(s) 582
RsaNI GTAC 1 cut(s) 581
RseI CAYNNNNRTG 3 cut(s) 90, 256, 588
SaqAI TTAA 4 cut(s) 63, 384, 426, 551
SatI GCNGC 1 cut(s) 192
Sau3AI GATC 2 cut(s) 484, 791
Sau96I GGNCC 3 cut(s) 284, 503, 732
SchI GAGTC 1 cut(s) 766
SduI GDGCHC 3 cut(s) 134, 210, 353
SfaNI GCATC 3 cut(s) 248, 565, 776
SfcI CTRYAG 2 cut(s) 121, 543
Sfr274I CTCGAG 1 cut(s) 370
SinI GGWCC 1 cut(s) 732
SlaI CTCGAG 1 cut(s) 370
SmiMI CAYNNNNRTG 3 cut(s) 90, 256, 588
SmlI CTYRAG 2 cut(s) 370, 801
SmoI CTYRAG 2 cut(s) 370, 801
Sse9I AATT 3 cut(s) 390, 477, 782
SsiI CCGC 1 cut(s) 15
SspI AATATT 1 cut(s) 680
SspMI CTAG 1 cut(s) 255
StyI CCWWGG 1 cut(s) 573
TaaI ACNGT 5 cut(s) 83, 205, 413, 458, 547
TaiI ACGT 1 cut(s) 711
TaqI TCGA 3 cut(s) 264, 371, 811
TaqII GACCGA 1 cut(s) 58
TasI AATT 3 cut(s) 390, 477, 782
TfiI GAWTC 3 cut(s) 49, 177, 403
Tru1I TTAA 4 cut(s) 63, 384, 426, 551
Tru9I TTAA 4 cut(s) 63, 384, 426, 551
TscAI CASTG 5 cut(s) 97, 418, 519, 552, 602
TseFI GTSAC 1 cut(s) 533
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 533
TspDTI ATGAA 2 cut(s) 38, 578
TspRI CASTG 5 cut(s) 97, 418, 519, 552, 602
VneI GTGCAC 1 cut(s) 349
VpaK11BI GGWCC 1 cut(s) 732
XapI RAATTY 1 cut(s) 782
XceI RCATGY 1 cut(s) 587
XcmI CCANNNNNNNNNTGG 1 cut(s) 170
XhoI CTCGAG 1 cut(s) 370
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.