AT2G40380

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0007014
Physical Location & Seq
Reverse (-)
16864446 .. 16865587
1142 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G40380.1

Sequence Viewer

Length: 642 bp
ATGTCTTCTTCTCCGGCGATTCTTCCCGTCACCAACCAGCAAGCAGCCACTCAATCTCAACCTCCAATCAATTCTCACGCCTTCCGTACTTTCCTCTCACGCCTCTCGTCCTCTCTCCGCGAAAGCCTCTCTCAACGCCGTCCATGGCTAGAACTCGTAGATCGGAGCTCCTTCGCTAGACCTGACTCTCTCACCGATTCCTTCTCCCGGATCCGTAAGAATCTCGCTTACTTCAAAGTCAATTACTCCGCAATTGTCTCGTTAGTCCTTGCTTTCTCCCTCCTCTCGCATCCATTCTCGCTTCTCGTCCTCCTCTCTCTCTTAGGCTCCTGGATGTTTCTCTACCTCTTCCGATCTTCAGATCAGCCTTTGGTTCTCTTTGGTCGTAGTTTCTCAGATCGTGAGACTTTGCTTGGTCTTGTTTTGACTACGATTGTGGTTGTGTTTATGACGAGTGTTGGATCTTTGTTAACATCGGCGTTAACGATTGGAATCGCGATTGTTTGCTTACATGGTGCGTTTAGGGTTCCTGATGATTTGTTCTTGGATGAACAAGAGCCTGCTAATGCTGGTTTACTCTCGTTCATTGGTAATTCTGCTGCTACTTCTGCTGCTGCTTCTGTTGTTGCAGGACGAGTTTGA

Protein Analysis

213

Amino Acids

23.16

Weight (kDa)

9.5

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 42 - 185 9.1e-49 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 120, 497
AciI CCGC 2 cut(s) 118, 249
AclWI GGATC 3 cut(s) 205, 218, 469
AcuI CTGAAG 1 cut(s) 342
AfaI GTAC 1 cut(s) 88
AfiI CCNNNNNNNGG 1 cut(s) 207
AgsI TTSAA 1 cut(s) 235
AjnI CCWGG 1 cut(s) 329
AluBI AGCT 1 cut(s) 168
AluI AGCT 1 cut(s) 168
Alw21I GWGCWC 1 cut(s) 170
Alw26I GTCTC 2 cut(s) 262, 398
AlwI GGATC 3 cut(s) 205, 218, 469
ApeKI GCWGC 4 cut(s) 44, 599, 611, 614
AsuC2I CCSGG 1 cut(s) 208
AsuHPI GGTGA 2 cut(s) 22, 184
BamHI GGATCC 1 cut(s) 210
BanII GRGCYC 1 cut(s) 170
Bbv12I GWGCWC 1 cut(s) 170
BbvI GCAGC 4 cut(s) 56, 586, 598, 601
BceAI ACGGC 1 cut(s) 123
BcgI CGANNNNNNTGC 2 cut(s) 240, 274
BciT130I CCWGG 1 cut(s) 331
BcnI CCSGG 1 cut(s) 208
BcoDI GTCTC 2 cut(s) 262, 398
BfaI CTAG 2 cut(s) 149, 177
BisI GCNGC 4 cut(s) 45, 600, 612, 615
BlsI GCNGC 4 cut(s) 46, 601, 613, 616
Bme1390I CCNGG 2 cut(s) 208, 331
BmiI GGNNCC 3 cut(s) 212, 328, 528
BmrFI CCNGG 2 cut(s) 208, 331
BmsI GCATC 1 cut(s) 298
BpuMI CCSGG 1 cut(s) 208
BsaBI GATNNNNATC 1 cut(s) 491
BsaJI CCNNGG 1 cut(s) 143
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse8I GATNNNNATC 1 cut(s) 491
BseBI CCWGG 1 cut(s) 331
BseDI CCNNGG 1 cut(s) 143
BseGI GGATG 3 cut(s) 289, 339, 553
BseJI GATNNNNATC 1 cut(s) 491
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMII CTCAG 1 cut(s) 408
BseRI GAGGAG 2 cut(s) 272, 302
BseXI GCAGC 4 cut(s) 56, 586, 598, 601
Bsh1236I CGCG 2 cut(s) 120, 497
BsiHKAI GWGCWC 1 cut(s) 170
BsiSI CCGG 2 cut(s) 14, 208
BslI CCNNNNNNNGG 1 cut(s) 207
BsmAI GTCTC 2 cut(s) 262, 398
Bsp1286I GDGCHC 1 cut(s) 170
Bsp143I GATC 6 cut(s) 160, 210, 353, 361, 397, 461
Bsp19I CCATGG 1 cut(s) 143
Bsp68I TCGCGA 1 cut(s) 497
BspACI CCGC 2 cut(s) 118, 249
BspCNI CTCAG 1 cut(s) 407
BspFNI CGCG 2 cut(s) 120, 497
BspLI GGNNCC 3 cut(s) 212, 328, 528
BspPI GGATC 3 cut(s) 205, 218, 469
BssECI CCNNGG 1 cut(s) 143
BssMI GATC 6 cut(s) 160, 210, 353, 361, 397, 461
BssT1I CCWWGG 1 cut(s) 143
Bst2UI CCWGG 1 cut(s) 331
Bst6I CTCTTC 1 cut(s) 353
BstC8I GCNNGC 2 cut(s) 42, 561
BstDEI CTNAG 2 cut(s) 322, 394
BstDSI CCRYGG 1 cut(s) 143
BstF5I GGATG 3 cut(s) 289, 339, 553
BstFNI CGCG 2 cut(s) 120, 497
BstKTI GATC 6 cut(s) 163, 213, 356, 364, 400, 464
BstMAI GTCTC 2 cut(s) 262, 398
BstMBI GATC 6 cut(s) 160, 210, 353, 361, 397, 461
BstMWI GCNNNNNNNGC 1 cut(s) 608
BstNI CCWGG 1 cut(s) 331
BstSCI CCNGG 2 cut(s) 206, 329
BstUI CGCG 2 cut(s) 120, 497
BstV1I GCAGC 4 cut(s) 56, 586, 598, 601
BstX2I RGATCY 2 cut(s) 210, 461
BstYI RGATCY 2 cut(s) 210, 461
BtgI CCRYGG 1 cut(s) 143
BtsCI GGATG 3 cut(s) 289, 339, 553
BtuMI TCGCGA 1 cut(s) 497
Cac8I GCNNGC 2 cut(s) 42, 561
Csp6I GTAC 1 cut(s) 87
CviAII CATG 2 cut(s) 144, 512
CviJI RGCY 7 cut(s) 47, 126, 148, 168, 327, 367, 559
CviKI_1 RGCY 7 cut(s) 47, 126, 148, 168, 327, 367, 559
CviQI GTAC 1 cut(s) 87
DdeI CTNAG 2 cut(s) 322, 394
DpnI GATC 6 cut(s) 162, 212, 355, 363, 399, 463
DpnII GATC 6 cut(s) 160, 210, 353, 361, 397, 461
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
Ecl136II GAGCTC 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 170
Eco53kI GAGCTC 1 cut(s) 168
Eco57I CTGAAG 1 cut(s) 342
EcoICRI GAGCTC 1 cut(s) 168
EcoRII CCWGG 1 cut(s) 329
EcoT14I CCWWGG 1 cut(s) 143
EcoT38I GRGCYC 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 143
FaeI CATG 2 cut(s) 147, 515
FaiI YATR 3 cut(s) 145, 449, 513
FatI CATG 2 cut(s) 143, 511
Fnu4HI GCNGC 4 cut(s) 45, 600, 612, 615
FokI GGATG 3 cut(s) 276, 346, 560
FriOI GRGCYC 1 cut(s) 170
Fsp4HI GCNGC 4 cut(s) 45, 600, 612, 615
FspBI CTAG 2 cut(s) 149, 177
GluI GCNGC 4 cut(s) 45, 600, 612, 615
HapII CCGG 2 cut(s) 14, 208
Hin1II CATG 2 cut(s) 147, 515
HincII GTYRAC 2 cut(s) 471, 483
HindII GTYRAC 2 cut(s) 471, 483
HinfI GANTC 5 cut(s) 19, 185, 197, 220, 492
HpaI GTTAAC 2 cut(s) 471, 483
HpaII CCGG 2 cut(s) 14, 208
HphI GGTGA 2 cut(s) 22, 184
Hpy166II GTNNAC 3 cut(s) 471, 483, 575
Hpy188I TCNGA 4 cut(s) 165, 353, 361, 397
Hpy188III TCNNGA 3 cut(s) 401, 496, 530
Hpy8I GTNNAC 3 cut(s) 471, 483, 575
HpyAV CCTTC 3 cut(s) 91, 181, 211
HpyCH4V TGCA 1 cut(s) 629
HpyF10VI GCNNNNNNNGC 1 cut(s) 608
HpyF3I CTNAG 2 cut(s) 322, 394
Hsp92II CATG 2 cut(s) 147, 515
KspAI GTTAAC 2 cut(s) 471, 483
Kzo9I GATC 6 cut(s) 160, 210, 353, 361, 397, 461
LmnI GCTCC 3 cut(s) 165, 173, 332
Lsp1109I GCAGC 4 cut(s) 56, 586, 598, 601
LweI GCATC 1 cut(s) 298
MaeI CTAG 2 cut(s) 149, 177
MaeIII GTNAC 1 cut(s) 28
MalI GATC 6 cut(s) 162, 212, 355, 363, 399, 463
MboI GATC 6 cut(s) 160, 210, 353, 361, 397, 461
MboII GAAGA 3 cut(s) 14, 340, 348
MfeI CAATTG 1 cut(s) 252
MflI RGATCY 2 cut(s) 210, 461
MhlI GDGCHC 1 cut(s) 170
MluCI AATT 4 cut(s) 70, 241, 252, 592
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 439
MseI TTAA 2 cut(s) 470, 482
MspI CCGG 2 cut(s) 14, 208
MspR9I CCNGG 2 cut(s) 208, 331
MunI CAATTG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 331
MvnI CGCG 2 cut(s) 120, 497
MwoI GCNNNNNNNGC 1 cut(s) 608
NciI CCSGG 1 cut(s) 208
NcoI CCATGG 1 cut(s) 143
NdeII GATC 6 cut(s) 160, 210, 353, 361, 397, 461
NlaIII CATG 2 cut(s) 147, 515
NlaIV GGNNCC 3 cut(s) 212, 328, 528
NmuCI GTSAC 1 cut(s) 28
NruI TCGCGA 1 cut(s) 497
PcsI WCGNNNNNNNCGW 1 cut(s) 482
PfeI GAWTC 4 cut(s) 19, 197, 220, 492
PfoI TCCNGGA 2 cut(s) 206, 329
PkrI GCNGC 4 cut(s) 46, 601, 613, 616
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
Psp124BI GAGCTC 1 cut(s) 170
Psp6I CCWGG 1 cut(s) 329
PspGI CCWGG 1 cut(s) 329
PspN4I GGNNCC 3 cut(s) 212, 328, 528
PsuI RGATCY 2 cut(s) 210, 461
RruI TCGCGA 1 cut(s) 497
RsaI GTAC 1 cut(s) 88
RsaNI GTAC 1 cut(s) 87
SacI GAGCTC 1 cut(s) 170
SaqAI TTAA 2 cut(s) 470, 482
SatI GCNGC 4 cut(s) 45, 600, 612, 615
Sau3AI GATC 6 cut(s) 160, 210, 353, 361, 397, 461
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 2 cut(s) 208, 331
SduI GDGCHC 1 cut(s) 170
SetI ASST 4 cut(s) 64, 170, 184, 348
SfaNI GCATC 1 cut(s) 298
Sse9I AATT 4 cut(s) 70, 241, 252, 592
SsiI CCGC 2 cut(s) 118, 249
SspMI CTAG 2 cut(s) 149, 177
SstI GAGCTC 1 cut(s) 170
StyD4I CCNGG 2 cut(s) 206, 329
StyI CCWWGG 1 cut(s) 143
TasI AATT 4 cut(s) 70, 241, 252, 592
TfiI GAWTC 4 cut(s) 19, 197, 220, 492
Tru1I TTAA 2 cut(s) 470, 482
Tru9I TTAA 2 cut(s) 470, 482
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 4 cut(s) 44, 599, 611, 614
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 2 cut(s) 564, 574
TspGWI ACGGA 2 cut(s) 74, 203
XspI CTAG 2 cut(s) 149, 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.