AT5G05380

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007014
Physical Location & Seq
Forward (+)
1592013 .. 1593185
1173 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G05380.2

Sequence Viewer

Length: 654 bp
ATGATGGCTAATCCTCCAACGTTACCGATCTCAGACCATTCCGGTGGTGGTTCCCAATCTCAGCAACCCGTATCAACACCGGCGTTTCGCACGTTCCTCTCTCGCCTCTCGTCGTCAATCCGCCAAAGCCTATCGCAGCGGCGTCCATGGTTAGAACTCGTAGATCGAAGCGCAATTTCACGTCCAGAGTCTCTCACCGATGCGTATTCTCGGATCCGGAGGAATCTCCCTTACTTCAAGGTAAACTACGTGACGATCGTCTCCTTGGTGCTCGCGCTCTCGCTCCTCTCCCATCCTTTCTCTCTTCTCGTTCTCCTATGCCTCTTCTGCGCTTGGATCTTCCTCTACCTTTTCCGTCCGTCAGATCAACCTCTGGTCGTCCTCGGCCGTACGTTTTCAGATCGTGAGACGCTTGGTGTTTTGGTAATTTTGACAATCGTCGTCGTCTTTTTAACCAGCGTTGGGTCTTTATTGACCTCTGCTTTGATGATTGGTTTCGGAATCGTATGCTTACACGGCGCGTTTAGAGTCCCGGAGGATCTTTTCTTGGATGATCAAGAACCTGCTAACACCGGCTTATTGTCTTTCCTTAGCGGAGCCGCCACTTCCGCCGCAGTTGCCGCCGCATCAACTCCTGCGTCAGGTCGTGTTTGA

Protein Analysis

217

Amino Acids

23.53

Weight (kDa)

9.02

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 110 - 252 3.8e-49 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 571
AccII CGCG 2 cut(s) 275, 521
AccIII TCCGGA 1 cut(s) 216
AciI CCGC 8 cut(s) 121, 139, 594, 600, 609, 612, 621, 624
AclI AACGTT 1 cut(s) 20
AclWI GGATC 4 cut(s) 208, 221, 344, 546
AcoI YGGCCR 1 cut(s) 385
AcyI GRCGYC 1 cut(s) 142
AfaI GTAC 1 cut(s) 391
AfiI CCNNNNNNNGG 2 cut(s) 462, 641
AgsI TTSAA 1 cut(s) 238
AjiI CACGTC 1 cut(s) 182
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 3 cut(s) 195, 265, 401
AlwI GGATC 4 cut(s) 208, 221, 344, 546
Aor13HI TCCGGA 1 cut(s) 216
AoxI GGCC 1 cut(s) 385
ApeKI GCWGC 1 cut(s) 136
ArsI GACNNNNNNTTYG 2 cut(s) 466, 498
AspLEI GCGC 4 cut(s) 173, 277, 332, 521
AsuC2I CCSGG 1 cut(s) 533
AsuHPI GGTGA 1 cut(s) 187
BamHI GGATCC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 1 cut(s) 148
BccI CCATC 1 cut(s) 300
BceAI ACGGC 2 cut(s) 372, 532
BclI TGATCA 1 cut(s) 553
BcnI CCSGG 1 cut(s) 533
BcoDI GTCTC 3 cut(s) 195, 265, 401
BfuAI ACCTGC 1 cut(s) 571
BisI GCNGC 6 cut(s) 137, 140, 600, 612, 621, 624
BlsI GCNGC 6 cut(s) 138, 141, 601, 613, 622, 625
Bme1390I CCNGG 1 cut(s) 533
BmgBI CACGTC 1 cut(s) 182
BmiI GGNNCC 3 cut(s) 52, 215, 598
BmrFI CCNGG 1 cut(s) 533
BmsI GCATC 2 cut(s) 190, 635
BoxI GACNNNNGTC 2 cut(s) 257, 437
Bpu10I CCTNAGC 1 cut(s) 590
BpuMI CCSGG 1 cut(s) 533
BsaAI YACGTR 1 cut(s) 250
BsaHI GRCGYC 1 cut(s) 142
BsaJI CCNNGG 3 cut(s) 146, 264, 382
BsaWI WCCGGW 2 cut(s) 41, 216
Bsc4I CCNNNNNNNGG 2 cut(s) 462, 641
Bse118I RCCGGY 2 cut(s) 79, 572
BseAI TCCGGA 1 cut(s) 216
BseDI CCNNGG 3 cut(s) 146, 264, 382
BseGI GGATG 2 cut(s) 292, 556
BseLI CCNNNNNNNGG 2 cut(s) 462, 641
BseMII CTCAG 2 cut(s) 45, 74
BseRI GAGGAG 1 cut(s) 275
BseX3I CGGCCG 1 cut(s) 385
BseXI GCAGC 1 cut(s) 148
Bsh1236I CGCG 2 cut(s) 275, 521
Bsh1285I CGRYCG 2 cut(s) 258, 388
BshFI GGCC 1 cut(s) 387
BsiEI CGRYCG 2 cut(s) 258, 388
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 5 cut(s) 42, 80, 217, 533, 573
BsiWI CGTACG 1 cut(s) 389
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 2 cut(s) 462, 641
BsmAI GTCTC 3 cut(s) 195, 265, 401
BsmBI CGTCTC 2 cut(s) 265, 401
BsmFI GGGAC 1 cut(s) 515
BsnI GGCC 1 cut(s) 387
Bsp1286I GDGCHC 1 cut(s) 273
Bsp13I TCCGGA 1 cut(s) 216
Bsp143I GATC 9 cut(s) 27, 163, 213, 255, 336, 364, 400, 538, 553
Bsp19I CCATGG 1 cut(s) 146
BspACI CCGC 8 cut(s) 121, 139, 594, 600, 609, 612, 621, 624
BspANI GGCC 1 cut(s) 387
BspCNI CTCAG 2 cut(s) 44, 73
BspEI TCCGGA 1 cut(s) 216
BspFNI CGCG 2 cut(s) 275, 521
BspLI GGNNCC 3 cut(s) 52, 215, 598
BspMI ACCTGC 1 cut(s) 571
BspPI GGATC 4 cut(s) 208, 221, 344, 546
BsrFI RCCGGY 2 cut(s) 79, 572
BssAI RCCGGY 2 cut(s) 79, 572
BssECI CCNNGG 3 cut(s) 146, 264, 382
BssMI GATC 9 cut(s) 27, 163, 213, 255, 336, 364, 400, 538, 553
BssNI GRCGYC 1 cut(s) 142
BssT1I CCWWGG 2 cut(s) 146, 264
Bst6I CTCTTC 2 cut(s) 309, 329
BstACI GRCGYC 1 cut(s) 142
BstBAI YACGTR 1 cut(s) 250
BstC8I GCNNGC 1 cut(s) 273
BstDEI CTNAG 3 cut(s) 31, 60, 590
BstDSI CCRYGG 1 cut(s) 146
BstENI CCTNNNNNAGG 1 cut(s) 639
BstF5I GGATG 2 cut(s) 292, 556
BstFNI CGCG 2 cut(s) 275, 521
BstHHI GCGC 4 cut(s) 173, 277, 332, 521
BstKTI GATC 9 cut(s) 30, 166, 216, 258, 339, 367, 403, 541, 556
BstMAI GTCTC 3 cut(s) 195, 265, 401
BstMBI GATC 9 cut(s) 27, 163, 213, 255, 336, 364, 400, 538, 553
BstMCI CGRYCG 2 cut(s) 258, 388
BstMWI GCNNNNNNNGC 5 cut(s) 327, 516, 608, 617, 620
BstPAI GACNNNNGTC 2 cut(s) 257, 437
BstSCI CCNGG 1 cut(s) 531
BstUI CGCG 2 cut(s) 275, 521
BstV1I GCAGC 1 cut(s) 148
BstX2I RGATCY 3 cut(s) 213, 336, 538
BstXI CCANNNNNNTGG 1 cut(s) 44
BstYI RGATCY 3 cut(s) 213, 336, 538
BstZI CGGCCG 1 cut(s) 385
BsuRI GGCC 1 cut(s) 387
BtgI CCRYGG 1 cut(s) 146
BtrI CACGTC 1 cut(s) 182
BtsCI GGATG 2 cut(s) 292, 556
BveI ACCTGC 1 cut(s) 571
Cac8I GCNNGC 1 cut(s) 273
CfoI GCGC 4 cut(s) 173, 277, 332, 521
Cfr10I RCCGGY 2 cut(s) 79, 572
CseI GACGC 3 cut(s) 131, 418, 627
Csp6I GTAC 1 cut(s) 390
CviAII CATG 1 cut(s) 147
CviJI RGCY 5 cut(s) 8, 129, 387, 576, 599
CviKI_1 RGCY 5 cut(s) 8, 129, 387, 576, 599
CviQI GTAC 1 cut(s) 390
DdeI CTNAG 3 cut(s) 31, 60, 590
DpnI GATC 9 cut(s) 29, 165, 215, 257, 338, 366, 402, 540, 555
DpnII GATC 9 cut(s) 27, 163, 213, 255, 336, 364, 400, 538, 553
EaeI YGGCCR 1 cut(s) 385
EagI CGGCCG 1 cut(s) 385
Eam1104I CTCTTC 2 cut(s) 309, 329
EarI CTCTTC 2 cut(s) 309, 329
EciI GGCGGA 2 cut(s) 110, 598
EclXI CGGCCG 1 cut(s) 385
Eco130I CCWWGG 2 cut(s) 146, 264
Eco52I CGGCCG 1 cut(s) 385
EcoNI CCTNNNNNAGG 1 cut(s) 639
EcoT14I CCWWGG 2 cut(s) 146, 264
ErhI CCWWGG 2 cut(s) 146, 264
Esp3I CGTCTC 2 cut(s) 265, 401
FaeI CATG 1 cut(s) 150
FaiI YATR 3 cut(s) 148, 319, 508
FaqI GGGAC 1 cut(s) 515
FatI CATG 1 cut(s) 146
FbaI TGATCA 1 cut(s) 553
Fnu4HI GCNGC 6 cut(s) 137, 140, 600, 612, 621, 624
FokI GGATG 2 cut(s) 279, 563
Fsp4HI GCNGC 6 cut(s) 137, 140, 600, 612, 621, 624
GlaI GCGC 4 cut(s) 172, 276, 331, 520
GluI GCNGC 6 cut(s) 137, 140, 600, 612, 621, 624
HaeIII GGCC 1 cut(s) 387
HapII CCGG 5 cut(s) 42, 80, 217, 533, 573
HgaI GACGC 3 cut(s) 131, 418, 627
HhaI GCGC 4 cut(s) 173, 277, 332, 521
Hin1I GRCGYC 1 cut(s) 142
Hin1II CATG 1 cut(s) 150
Hin6I GCGC 4 cut(s) 171, 275, 330, 519
HinP1I GCGC 4 cut(s) 171, 275, 330, 519
HinfI GANTC 4 cut(s) 188, 223, 501, 528
HpaII CCGG 5 cut(s) 42, 80, 217, 533, 573
HphI GGTGA 1 cut(s) 187
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 5 cut(s) 34, 213, 364, 400, 500
Hpy188III TCNNGA 4 cut(s) 185, 217, 404, 557
Hpy8I GTNNAC 1 cut(s) 244
Hpy99I CGWCG 3 cut(s) 115, 443, 446
HpyCH4IV ACGT 5 cut(s) 20, 92, 181, 249, 392
HpyF10VI GCNNNNNNNGC 5 cut(s) 327, 516, 608, 617, 620
HpyF3I CTNAG 3 cut(s) 31, 60, 590
HpySE526I ACGT 5 cut(s) 20, 92, 181, 249, 392
Hsp92I GRCGYC 1 cut(s) 142
Hsp92II CATG 1 cut(s) 150
HspAI GCGC 4 cut(s) 171, 275, 330, 519
Kpn2I TCCGGA 1 cut(s) 216
Ksp22I TGATCA 1 cut(s) 553
Kzo9I GATC 9 cut(s) 27, 163, 213, 255, 336, 364, 400, 538, 553
LmnI GCTCC 2 cut(s) 288, 596
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 2 cut(s) 190, 635
MaeII ACGT 5 cut(s) 20, 92, 181, 249, 392
MaeIII GTNAC 2 cut(s) 21, 250
MalI GATC 9 cut(s) 29, 165, 215, 257, 338, 366, 402, 540, 555
MboI GATC 9 cut(s) 27, 163, 213, 255, 336, 364, 400, 538, 553
MboII GAAGA 3 cut(s) 296, 316, 331
MflI RGATCY 3 cut(s) 213, 336, 538
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 2 cut(s) 174, 426
MlyI GAGTC 2 cut(s) 197, 537
MmeI TCCRAC 1 cut(s) 41
MroI TCCGGA 1 cut(s) 216
MseI TTAA 1 cut(s) 452
MslI CAYNNNNRTG 1 cut(s) 42
MspA1I CMGCKG 1 cut(s) 139
MspI CCGG 5 cut(s) 42, 80, 217, 533, 573
MspR9I CCNGG 1 cut(s) 533
MvnI CGCG 2 cut(s) 275, 521
MwoI GCNNNNNNNGC 5 cut(s) 327, 516, 608, 617, 620
NciI CCSGG 1 cut(s) 533
NcoI CCATGG 1 cut(s) 146
NdeII GATC 9 cut(s) 27, 163, 213, 255, 336, 364, 400, 538, 553
NlaIII CATG 1 cut(s) 150
NlaIV GGNNCC 3 cut(s) 52, 215, 598
NmeAIII GCCGAG 1 cut(s) 363
NmuCI GTSAC 1 cut(s) 250
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PfeI GAWTC 2 cut(s) 223, 501
Pfl23II CGTACG 1 cut(s) 389
PfoI TCCNGGA 1 cut(s) 531
PkrI GCNGC 6 cut(s) 138, 141, 601, 613, 622, 625
Ple19I CGATCG 1 cut(s) 258
PleI GAGTC 2 cut(s) 196, 536
PpsI GAGTC 2 cut(s) 196, 536
Ppu21I YACGTR 1 cut(s) 250
PshAI GACNNNNGTC 2 cut(s) 257, 437
Psp1406I AACGTT 1 cut(s) 20
PspLI CGTACG 1 cut(s) 389
PspN4I GGNNCC 3 cut(s) 52, 215, 598
PsuI RGATCY 3 cut(s) 213, 336, 538
PvuI CGATCG 1 cut(s) 258
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
RseI CAYNNNNRTG 1 cut(s) 42
SaqAI TTAA 1 cut(s) 452
SatI GCNGC 6 cut(s) 137, 140, 600, 612, 621, 624
Sau3AI GATC 9 cut(s) 27, 163, 213, 255, 336, 364, 400, 538, 553
SchI GAGTC 2 cut(s) 197, 537
ScrFI CCNGG 1 cut(s) 533
SduI GDGCHC 1 cut(s) 273
SfaNI GCATC 2 cut(s) 190, 635
SgrAI CRCCGGYG 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 42
Sse9I AATT 2 cut(s) 174, 426
SsiI CCGC 8 cut(s) 121, 139, 594, 600, 609, 612, 621, 624
StyD4I CCNGG 1 cut(s) 531
StyI CCWWGG 2 cut(s) 146, 264
TaiI ACGT 5 cut(s) 23, 95, 184, 252, 395
TaqI TCGA 1 cut(s) 166
TasI AATT 2 cut(s) 174, 426
TauI GCSGC 5 cut(s) 142, 602, 614, 623, 626
TfiI GAWTC 2 cut(s) 223, 501
Tru1I TTAA 1 cut(s) 452
Tru9I TTAA 1 cut(s) 452
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 1 cut(s) 136
Tsp45I GTSAC 1 cut(s) 250
TspGWI ACGGA 2 cut(s) 344, 348
XagI CCTNNNNNAGG 1 cut(s) 639
XcmI CCANNNNNNNNNTGG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.