AT2G42220

Rhodanese-like domain-containing protein 9, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012197
Physical Location & Seq
Forward (+)
17592038 .. 17593500
1463 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G42220.1

Sequence Viewer

Length: 705 bp
ATGGCGGGGATCATAAGCCCTAGCCCTACGGCTCTTTATTTCACCAGTAATGTCGGTGGGAGGCGACTGAAAGCAGTGAGCTGGGCGGGAAAGAGTGTCTCCGGGAACGTTATCCGCCGGAGAAGCTTGAGAATTGCTGCGGAGCTGAAATTCGTGAATGCAGAAGAAGCAAAACAGTTAATAGCTGAAGAAGGTTACTCGGTGGTGGATGTAAGAGACAAGACTCAATTCGAGAGAGCTCATATAAAATCTTGCTCCCATATTCCTCTCTTCATTTACAACGAAGACAACGATATTGGCACGATCATAAAGAGGACAGTGCACAACAATTTCTCAGGGCTCTTCTTTGGTTTACCTTTCACGAAAGTGAATCCGGAATTTCTTAAATCTGTTAGAAACGAGTTTTCTCAAGACAGCAAACTTTTACTTGTTTGCCAAGAAGGTCTCAGATCTGCAGCTGCAGCTAGTAGATTGGAGGAAGCAGGTTACGAAAACATTGCTTGTGTAACATCAGGGCTACAATCTGTAAAACCAGGGACATTTGAATCCGTCGGTTCCACTGAGTTGCAGAATGCAGGCAAAGCAGGGCTTATCACAATTCAAGGCAAGATCTCAGCAGTCTTAGGGACAGTACTCGTCTGTGCTTATTTGTTCATACAGTTCTTCCCGGACCAAGCAGAGAAGCTCTTTCCTCCAACAAGCTAA

Protein Analysis

234

Amino Acids

25.51

Weight (kDa)

8.87

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 54 - 175 3.8e-12 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0012197)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42220
fragaria_vesca FvH4_6g22700 FvH4_6g22700 FvH4_6g22700
malus_domestica MD12G1061100.v1.1 MD14G1060800.v1.1
prunus_persica Prupe.7G073600_v2.0.a1 Prupe.7G073600_v2.0.a1
pyrus_communis pycom12g05400
rosa_chinensis RchiOBHm_Chr3g0480311
rosa_laevigata RLG00000023493
rosa_multiflora Rmu_ssc0000353.1_g000001
rosa_roxburghii Rroxscaffold_6G00401570
rosa_rugosa Rorug03G0182400
rosa_samantha Rh3AG234300 Rh3BG266600 Rh3CG264100 Rh3DG259600
rosa_wichuraiana Rw3G021030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 473
AccIII TCCGGA 1 cut(s) 373
AciI CCGC 4 cut(s) 5, 86, 115, 140
AclI AACGTT 1 cut(s) 108
AclWI GGATC 1 cut(s) 17
AcsI RAATTY 2 cut(s) 149, 377
AcuI CTGAAG 1 cut(s) 207
AfaI GTAC 1 cut(s) 633
AgsI TTSAA 2 cut(s) 545, 602
AjnI CCWGG 1 cut(s) 532
AleI CACNNNNGTG 1 cut(s) 365
AluBI AGCT 9 cut(s) 81, 126, 145, 185, 239, 458, 464, 685, 702
AluI AGCT 9 cut(s) 81, 126, 145, 185, 239, 458, 464, 685, 702
Alw21I GWGCWC 2 cut(s) 241, 324
Alw26I GTCTC 3 cut(s) 103, 210, 449
Alw44I GTGCAC 1 cut(s) 320
AlwI GGATC 1 cut(s) 17
Aor13HI TCCGGA 1 cut(s) 373
ApaLI GTGCAC 1 cut(s) 320
ApeKI GCWGC 4 cut(s) 137, 455, 458, 461
ApoI RAATTY 2 cut(s) 149, 377
AspS9I GGNCC 1 cut(s) 670
AsuC2I CCSGG 2 cut(s) 103, 668
AsuHPI GGTGA 1 cut(s) 34
AvaII GGWCC 1 cut(s) 670
BaeGI GKGCMC 1 cut(s) 324
BanII GRGCYC 2 cut(s) 241, 342
BbsI GAAGAC 1 cut(s) 291
Bbv12I GWGCWC 2 cut(s) 241, 324
BbvI GCAGC 4 cut(s) 124, 445, 467, 473
BceAI ACGGC 1 cut(s) 45
BcgI CGANNNNNNTGC 2 cut(s) 479, 513
BciT130I CCWGG 1 cut(s) 534
BcnI CCSGG 2 cut(s) 103, 668
BcoDI GTCTC 3 cut(s) 103, 210, 449
BfaI CTAG 2 cut(s) 21, 465
BfmI CTRYAG 2 cut(s) 453, 459
BfuAI ACCTGC 1 cut(s) 473
BglII AGATCT 2 cut(s) 449, 609
BisI GCNGC 4 cut(s) 138, 456, 459, 462
BlsI GCNGC 4 cut(s) 139, 457, 460, 463
BmcAI AGTACT 1 cut(s) 633
Bme1390I CCNGG 3 cut(s) 103, 534, 668
Bme18I GGWCC 1 cut(s) 670
BmgT120I GGNCC 1 cut(s) 670
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 3 cut(s) 103, 534, 668
BpiI GAAGAC 1 cut(s) 291
BpuEI CTTGAG 2 cut(s) 148, 393
BpuMI CCSGG 2 cut(s) 103, 668
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 1 cut(s) 533
BsaWI WCCGGW 1 cut(s) 373
Bse1I ACTGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 495
BseAI TCCGGA 1 cut(s) 373
BseBI CCWGG 1 cut(s) 534
BseDI CCNNGG 1 cut(s) 533
BseGI GGATG 1 cut(s) 214
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 4 cut(s) 348, 460, 552, 627
BseNI ACTGG 1 cut(s) 45
BseSI GKGCMC 1 cut(s) 324
BseXI GCAGC 4 cut(s) 124, 445, 467, 473
BseYI CCCAGC 1 cut(s) 81
BsiHKAI GWGCWC 2 cut(s) 241, 324
BsiSI CCGG 4 cut(s) 102, 118, 374, 668
BslFI GGGAC 2 cut(s) 550, 640
BsmAI GTCTC 3 cut(s) 103, 210, 449
BsmFI GGGAC 2 cut(s) 550, 640
BsmI GAATGC 2 cut(s) 163, 577
Bso31I GGTCTC 1 cut(s) 449
Bsp1286I GDGCHC 3 cut(s) 241, 324, 342
Bsp13I TCCGGA 1 cut(s) 373
Bsp143I GATC 4 cut(s) 9, 303, 449, 609
BspACI CCGC 4 cut(s) 5, 86, 115, 140
BspCNI CTCAG 4 cut(s) 347, 459, 553, 626
BspEI TCCGGA 1 cut(s) 373
BspLI GGNNCC 1 cut(s) 556
BspMAI CTGCAG 2 cut(s) 457, 463
BspMI ACCTGC 1 cut(s) 473
BspPI GGATC 1 cut(s) 17
BspQI GCTCTTC 1 cut(s) 347
BspTNI GGTCTC 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 495
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 1 cut(s) 533
BssMI GATC 4 cut(s) 9, 303, 449, 609
Bst2UI CCWGG 1 cut(s) 534
Bst4CI ACNGT 4 cut(s) 177, 319, 631, 660
Bst6I CTCTTC 2 cut(s) 275, 347
BstC8I GCNNGC 1 cut(s) 577
BstDEI CTNAG 5 cut(s) 334, 446, 561, 613, 622
BstF5I GGATG 1 cut(s) 214
BstKTI GATC 4 cut(s) 12, 306, 452, 612
BstMAI GTCTC 3 cut(s) 103, 210, 449
BstMBI GATC 4 cut(s) 9, 303, 449, 609
BstMWI GCNNNNNNNGC 4 cut(s) 123, 167, 461, 581
BstNI CCWGG 1 cut(s) 534
BstSCI CCNGG 3 cut(s) 101, 532, 666
BstSFI CTRYAG 2 cut(s) 453, 459
BstSLI GKGCMC 1 cut(s) 324
BstV1I GCAGC 4 cut(s) 124, 445, 467, 473
BstV2I GAAGAC 1 cut(s) 291
BstX2I RGATCY 2 cut(s) 449, 609
BstYI RGATCY 2 cut(s) 449, 609
BtsCI GGATG 1 cut(s) 214
BtsI GCAGTG 1 cut(s) 81
BtsIMutI CAGTG 3 cut(s) 81, 324, 558
BveI ACCTGC 1 cut(s) 473
Cac8I GCNNGC 1 cut(s) 577
Cfr13I GGNCC 1 cut(s) 670
Csp6I GTAC 1 cut(s) 632
CviQI GTAC 1 cut(s) 632
DdeI CTNAG 5 cut(s) 334, 446, 561, 613, 622
DpnI GATC 4 cut(s) 11, 305, 451, 611
DpnII GATC 4 cut(s) 9, 303, 449, 609
Eam1104I CTCTTC 2 cut(s) 275, 347
EarI CTCTTC 2 cut(s) 275, 347
EciI GGCGGA 1 cut(s) 104
Ecl136II GAGCTC 1 cut(s) 239
Eco24I GRGCYC 2 cut(s) 241, 342
Eco31I GGTCTC 1 cut(s) 449
Eco47I GGWCC 1 cut(s) 670
Eco53kI GAGCTC 1 cut(s) 239
Eco57I CTGAAG 1 cut(s) 207
EcoICRI GAGCTC 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 532
EcoT38I GRGCYC 2 cut(s) 241, 342
FaiI YATR 6 cut(s) 14, 243, 245, 261, 308, 656
FalI AAGNNNNNCTT 2 cut(s) 573, 605
FaqI GGGAC 2 cut(s) 550, 640
FauI CCCGC 1 cut(s) 79
Fnu4HI GCNGC 4 cut(s) 138, 456, 459, 462
FokI GGATG 1 cut(s) 221
FriOI GRGCYC 2 cut(s) 241, 342
Fsp4HI GCNGC 4 cut(s) 138, 456, 459, 462
FspBI CTAG 2 cut(s) 21, 465
GluI GCNGC 4 cut(s) 138, 456, 459, 462
GsaI CCCAGC 1 cut(s) 85
HapII CCGG 4 cut(s) 102, 118, 374, 668
HindIII AAGCTT 1 cut(s) 124
HinfI GANTC 3 cut(s) 223, 370, 545
HpaII CCGG 4 cut(s) 102, 118, 374, 668
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 2 cut(s) 322, 353
Hpy188I TCNGA 1 cut(s) 449
Hpy188III TCNNGA 5 cut(s) 154, 232, 361, 374, 410
Hpy8I GTNNAC 2 cut(s) 322, 353
Hpy99I CGWCG 1 cut(s) 554
HpyAV CCTTC 2 cut(s) 185, 434
HpyCH4III ACNGT 4 cut(s) 177, 319, 631, 660
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 6 cut(s) 161, 322, 455, 461, 568, 575
HpyF10VI GCNNNNNNNGC 4 cut(s) 123, 167, 461, 581
HpyF3I CTNAG 5 cut(s) 334, 446, 561, 613, 622
HpySE526I ACGT 1 cut(s) 108
Kpn2I TCCGGA 1 cut(s) 373
Kzo9I GATC 4 cut(s) 9, 303, 449, 609
LguI GCTCTTC 1 cut(s) 347
LmnI GCTCC 2 cut(s) 142, 260
Lsp1109I GCAGC 4 cut(s) 124, 445, 467, 473
MaeI CTAG 2 cut(s) 21, 465
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 3 cut(s) 194, 485, 505
MalI GATC 4 cut(s) 11, 305, 451, 611
MboI GATC 4 cut(s) 9, 303, 449, 609
MboII GAAGA 6 cut(s) 176, 200, 262, 296, 334, 655
MflI RGATCY 2 cut(s) 449, 609
MhlI GDGCHC 3 cut(s) 241, 324, 342
MluCI AATT 6 cut(s) 132, 149, 227, 328, 377, 597
MlyI GAGTC 1 cut(s) 217
MnlI CCTC 5 cut(s) 54, 276, 306, 469, 702
MroI TCCGGA 1 cut(s) 373
MseI TTAA 2 cut(s) 179, 384
MslI CAYNNNNRTG 1 cut(s) 365
MspA1I CMGCKG 1 cut(s) 458
MspI CCGG 4 cut(s) 102, 118, 374, 668
MspR9I CCNGG 3 cut(s) 103, 534, 668
Mva1269I GAATGC 2 cut(s) 163, 577
MvaI CCWGG 1 cut(s) 534
MwoI GCNNNNNNNGC 4 cut(s) 123, 167, 461, 581
NciI CCSGG 2 cut(s) 103, 668
NdeII GATC 4 cut(s) 9, 303, 449, 609
NlaIV GGNNCC 1 cut(s) 556
OliI CACNNNNGTG 1 cut(s) 365
PciSI GCTCTTC 1 cut(s) 347
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PctI GAATGC 2 cut(s) 163, 577
PfeI GAWTC 2 cut(s) 370, 545
PfoI TCCNGGA 2 cut(s) 101, 666
PkrI GCNGC 4 cut(s) 139, 457, 460, 463
PleI GAGTC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 217
Psp124BI GAGCTC 1 cut(s) 241
Psp1406I AACGTT 1 cut(s) 108
Psp6I CCWGG 1 cut(s) 532
PspFI CCCAGC 1 cut(s) 81
PspGI CCWGG 1 cut(s) 532
PspN4I GGNNCC 1 cut(s) 556
PspPI GGNCC 1 cut(s) 670
PstI CTGCAG 2 cut(s) 457, 463
PsuI RGATCY 2 cut(s) 449, 609
PvuII CAGCTG 1 cut(s) 458
RsaI GTAC 1 cut(s) 633
RsaNI GTAC 1 cut(s) 632
RseI CAYNNNNRTG 1 cut(s) 365
SacI GAGCTC 1 cut(s) 241
SapI GCTCTTC 1 cut(s) 347
SaqAI TTAA 2 cut(s) 179, 384
SatI GCNGC 4 cut(s) 138, 456, 459, 462
Sau3AI GATC 4 cut(s) 9, 303, 449, 609
Sau96I GGNCC 1 cut(s) 670
ScaI AGTACT 1 cut(s) 633
SchI GAGTC 1 cut(s) 217
ScrFI CCNGG 3 cut(s) 103, 534, 668
SduI GDGCHC 3 cut(s) 241, 324, 342
SfcI CTRYAG 2 cut(s) 453, 459
SinI GGWCC 1 cut(s) 670
SmiMI CAYNNNNRTG 1 cut(s) 365
SmlI CTYRAG 2 cut(s) 127, 408
SmoI CTYRAG 2 cut(s) 127, 408
Sse9I AATT 6 cut(s) 132, 149, 227, 328, 377, 597
SsiI CCGC 4 cut(s) 5, 86, 115, 140
SspMI CTAG 2 cut(s) 21, 465
SstI GAGCTC 1 cut(s) 241
StyD4I CCNGG 3 cut(s) 101, 532, 666
TaaI ACNGT 4 cut(s) 177, 319, 631, 660
TaiI ACGT 1 cut(s) 111
TaqI TCGA 1 cut(s) 231
TasI AATT 6 cut(s) 132, 149, 227, 328, 377, 597
TatI WGTACW 1 cut(s) 631
TfiI GAWTC 2 cut(s) 370, 545
Tru1I TTAA 2 cut(s) 179, 384
Tru9I TTAA 2 cut(s) 179, 384
TscAI CASTG 3 cut(s) 81, 324, 565
TseI GCWGC 4 cut(s) 137, 455, 458, 461
TspDTI ATGAA 2 cut(s) 262, 643
TspGWI ACGGA 1 cut(s) 538
TspRI CASTG 3 cut(s) 81, 324, 565
VneI GTGCAC 1 cut(s) 320
VpaK11BI GGWCC 1 cut(s) 670
XapI RAATTY 2 cut(s) 149, 377
XspI CTAG 2 cut(s) 21, 465
ZrmI AGTACT 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.