Rh3BG266600

Rhodanese-like domain-containing protein 9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0012197
Physical Location & Seq
Forward (+)
25832743 .. 25834823
2081 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG266600.1

Sequence Viewer

Length: 546 bp
ATGGCAGGAATTGCGTTTGGCACTTGCTGTTCAACTATCTCTTCCACCAGCAATTTCCGCACTTCTTGGTTGAAACTTGAGACTAGTACTCATCGTGGAGGAACCATCTTAAAGAAGCCAAACAGTGTTAGAACTTTGGCAATTAGAGCAGAAGTGAACTTTGTAAGTGCAGACGAAGCTAAAGAACTAATTGCAGAAGGATATACGGTTATTGATGTTCGGGACAAATCTCAATATGATCGAGCTCATATCAAATCATGTTATCATGTGCCTCTTTTCATCGAAAATCAAGACAAAGACATCGGCACAATTGTAAAGAGGACTGTGCACAACAATTTCTCAGGTCTATTCTTCGGGTTACCTTTCACTAAACCCAACCCTGAGTTTGTACAATCTGTTAAAGGCCAGTTTTCACCTCAGAGTAAACTGTTACTGGTGTGCCAGGAAGGATTGAGGTCTGCAGCTGCTGCTAGTAGGTTGGAGCAGGCTGGTTTCCAAGACATAGCATGTATAACATCAGGGCTGCAAAAAGTAAAACCAGGTTAG

Protein Analysis

181

Amino Acids

19.84

Weight (kDa)

9.16

Isoelectric Point (pI)

37.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 56 - 178 4.3e-11 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012197)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42220
fragaria_vesca FvH4_6g22700 FvH4_6g22700 FvH4_6g22700
malus_domestica MD12G1061100.v1.1 MD14G1060800.v1.1
prunus_persica Prupe.7G073600_v2.0.a1 Prupe.7G073600_v2.0.a1
pyrus_communis pycom12g05400
rosa_chinensis RchiOBHm_Chr3g0480311
rosa_laevigata RLG00000023493
rosa_multiflora Rmu_ssc0000353.1_g000001
rosa_roxburghii Rroxscaffold_6G00401570
rosa_rugosa Rorug03G0182400
rosa_samantha Rh3AG234300 Rh3BG266600 Rh3CG264100 Rh3DG259600
rosa_wichuraiana Rw3G021030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 58
AfaI GTAC 2 cut(s) 88, 390
AgsI TTSAA 2 cut(s) 33, 73
AhlI ACTAGT 1 cut(s) 83
AjnI CCWGG 2 cut(s) 441, 538
AluBI AGCT 3 cut(s) 179, 245, 464
AluI AGCT 3 cut(s) 179, 245, 464
Alw21I GWGCWC 2 cut(s) 247, 330
Alw26I GTCTC 1 cut(s) 74
Alw44I GTGCAC 1 cut(s) 326
AlwNI CAGNNNCTG 1 cut(s) 467
AoxI GGCC 1 cut(s) 403
ApaLI GTGCAC 1 cut(s) 326
ApeKI GCWGC 4 cut(s) 461, 464, 467, 523
AsuHPI GGTGA 1 cut(s) 405
BaeGI GKGCMC 1 cut(s) 330
BanII GRGCYC 1 cut(s) 247
Bbv12I GWGCWC 2 cut(s) 247, 330
BbvI GCAGC 4 cut(s) 451, 454, 473, 510
BccI CCATC 1 cut(s) 113
BciT130I CCWGG 2 cut(s) 443, 540
BcoDI GTCTC 1 cut(s) 74
BcuI ACTAGT 1 cut(s) 83
BfaI CTAG 2 cut(s) 84, 471
BfmI CTRYAG 1 cut(s) 459
BisI GCNGC 4 cut(s) 462, 465, 468, 524
BlsI GCNGC 4 cut(s) 463, 466, 469, 525
BmcAI AGTACT 1 cut(s) 88
Bme1390I CCNGG 2 cut(s) 443, 540
BmiI GGNNCC 1 cut(s) 103
BmrFI CCNGG 2 cut(s) 443, 540
BpuEI CTTGAG 1 cut(s) 98
Bse1I ACTGG 2 cut(s) 406, 438
BseBI CCWGG 2 cut(s) 443, 540
BseMII CTCAG 3 cut(s) 354, 372, 431
BseNI ACTGG 2 cut(s) 406, 438
BseSI GKGCMC 1 cut(s) 330
BseXI GCAGC 4 cut(s) 451, 454, 473, 510
BsgI GTGCAG 1 cut(s) 189
BshFI GGCC 1 cut(s) 405
BsiHKAI GWGCWC 2 cut(s) 247, 330
BslFI GGGAC 1 cut(s) 236
BsmAI GTCTC 1 cut(s) 74
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 1 cut(s) 405
Bsp1286I GDGCHC 2 cut(s) 247, 330
Bsp1407I TGTACA 1 cut(s) 388
Bsp143I GATC 1 cut(s) 238
BspACI CCGC 1 cut(s) 58
BspANI GGCC 1 cut(s) 405
BspCNI CTCAG 3 cut(s) 353, 373, 430
BspLI GGNNCC 1 cut(s) 103
BspMAI CTGCAG 1 cut(s) 463
BsrGI TGTACA 1 cut(s) 388
BsrI ACTGG 2 cut(s) 406, 438
BssMI GATC 1 cut(s) 238
Bst2UI CCWGG 2 cut(s) 443, 540
Bst4CI ACNGT 4 cut(s) 125, 208, 325, 429
Bst6I CTCTTC 1 cut(s) 46
BstAPI GCANNNNNTGC 2 cut(s) 11, 467
BstAUI TGTACA 1 cut(s) 388
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 3 cut(s) 340, 381, 417
BstEII GGTNACC 1 cut(s) 357
BstKTI GATC 1 cut(s) 241
BstMAI GTCTC 1 cut(s) 74
BstMBI GATC 1 cut(s) 238
BstMWI GCNNNNNNNGC 5 cut(s) 11, 57, 146, 176, 467
BstNI CCWGG 2 cut(s) 443, 540
BstNSI RCATGY 1 cut(s) 510
BstPI GGTNACC 1 cut(s) 357
BstSCI CCNGG 2 cut(s) 441, 538
BstSFI CTRYAG 1 cut(s) 459
BstSLI GKGCMC 1 cut(s) 330
BstV1I GCAGC 4 cut(s) 451, 454, 473, 510
BsuRI GGCC 1 cut(s) 405
BtsIMutI CAGTG 1 cut(s) 130
Cac8I GCNNGC 1 cut(s) 486
CaiI CAGNNNCTG 1 cut(s) 467
CsiI ACCWGGT 1 cut(s) 538
Csp6I GTAC 2 cut(s) 87, 389
CviAII CATG 3 cut(s) 258, 266, 507
CviJI RGCY 7 cut(s) 118, 179, 245, 405, 464, 488, 523
CviKI_1 RGCY 7 cut(s) 118, 179, 245, 405, 464, 488, 523
CviQI GTAC 2 cut(s) 87, 389
DdeI CTNAG 3 cut(s) 340, 381, 417
DpnI GATC 1 cut(s) 240
DpnII GATC 1 cut(s) 238
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
Ecl136II GAGCTC 1 cut(s) 245
Eco24I GRGCYC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 245
Eco91I GGTNACC 1 cut(s) 357
EcoICRI GAGCTC 1 cut(s) 245
EcoO65I GGTNACC 1 cut(s) 357
EcoRII CCWGG 2 cut(s) 441, 538
EcoT38I GRGCYC 1 cut(s) 247
FaeI CATG 3 cut(s) 261, 269, 510
FaiI YATR 8 cut(s) 204, 237, 249, 259, 267, 503, 508, 512
FaqI GGGAC 1 cut(s) 236
FatI CATG 3 cut(s) 257, 265, 506
Fnu4HI GCNGC 4 cut(s) 462, 465, 468, 524
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 4 cut(s) 462, 465, 468, 524
FspBI CTAG 2 cut(s) 84, 471
GluI GCNGC 4 cut(s) 462, 465, 468, 524
HaeIII GGCC 1 cut(s) 405
Hin1II CATG 3 cut(s) 261, 269, 510
HphI GGTGA 1 cut(s) 405
Hpy166II GTNNAC 3 cut(s) 157, 328, 425
Hpy188I TCNGA 1 cut(s) 420
Hpy188III TCNNGA 2 cut(s) 221, 290
Hpy8I GTNNAC 3 cut(s) 157, 328, 425
HpyAV CCTTC 2 cut(s) 191, 440
HpyCH4III ACNGT 4 cut(s) 125, 208, 325, 429
HpyCH4V TGCA 5 cut(s) 170, 194, 328, 461, 526
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 57, 146, 176, 467
HpyF3I CTNAG 3 cut(s) 340, 381, 417
Hsp92II CATG 3 cut(s) 261, 269, 510
Kzo9I GATC 1 cut(s) 238
LmnI GCTCC 1 cut(s) 481
Lsp1109I GCAGC 4 cut(s) 451, 454, 473, 510
MabI ACCWGGT 1 cut(s) 538
MaeI CTAG 2 cut(s) 84, 471
MaeIII GTNAC 2 cut(s) 357, 429
MalI GATC 1 cut(s) 240
MboI GATC 1 cut(s) 238
MboII GAAGA 2 cut(s) 33, 343
MfeI CAATTG 1 cut(s) 309
MhlI GDGCHC 2 cut(s) 247, 330
MluCI AATT 6 cut(s) 9, 52, 141, 189, 309, 334
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 5 cut(s) 92, 282, 312, 426, 447
MseI TTAA 2 cut(s) 110, 399
MspA1I CMGCKG 1 cut(s) 464
MspR9I CCNGG 2 cut(s) 443, 540
MunI CAATTG 1 cut(s) 309
MvaI CCWGG 2 cut(s) 443, 540
MwoI GCNNNNNNNGC 5 cut(s) 11, 57, 146, 176, 467
NdeII GATC 1 cut(s) 238
NlaIII CATG 3 cut(s) 261, 269, 510
NlaIV GGNNCC 1 cut(s) 103
NspI RCATGY 1 cut(s) 510
PkrI GCNGC 4 cut(s) 463, 466, 469, 525
Psp124BI GAGCTC 1 cut(s) 247
Psp6I CCWGG 2 cut(s) 441, 538
PspEI GGTNACC 1 cut(s) 357
PspGI CCWGG 2 cut(s) 441, 538
PspN4I GGNNCC 1 cut(s) 103
PstI CTGCAG 1 cut(s) 463
PstNI CAGNNNCTG 1 cut(s) 467
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 2 cut(s) 88, 390
RsaNI GTAC 2 cut(s) 87, 389
SacI GAGCTC 1 cut(s) 247
SaqAI TTAA 2 cut(s) 110, 399
SatI GCNGC 4 cut(s) 462, 465, 468, 524
Sau3AI GATC 1 cut(s) 238
ScaI AGTACT 1 cut(s) 88
ScrFI CCNGG 2 cut(s) 443, 540
SduI GDGCHC 2 cut(s) 247, 330
SetI ASST 9 cut(s) 181, 247, 346, 364, 418, 458, 466, 479, 544
SexAI ACCWGGT 1 cut(s) 538
SfcI CTRYAG 1 cut(s) 459
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
SpeI ACTAGT 1 cut(s) 83
Sse9I AATT 6 cut(s) 9, 52, 141, 189, 309, 334
SsiI CCGC 1 cut(s) 58
SspMI CTAG 2 cut(s) 84, 471
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 2 cut(s) 441, 538
TaaI ACNGT 4 cut(s) 125, 208, 325, 429
TaqI TCGA 2 cut(s) 241, 282
TasI AATT 6 cut(s) 9, 52, 141, 189, 309, 334
TatI WGTACW 2 cut(s) 86, 388
Tru1I TTAA 2 cut(s) 110, 399
Tru9I TTAA 2 cut(s) 110, 399
TscAI CASTG 1 cut(s) 130
TseI GCWGC 4 cut(s) 461, 464, 467, 523
TspDTI ATGAA 1 cut(s) 268
TspRI CASTG 1 cut(s) 130
VneI GTGCAC 1 cut(s) 326
XceI RCATGY 1 cut(s) 510
XspI CTAG 2 cut(s) 84, 471
ZrmI AGTACT 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.