Rh3AG234300

Rhodanese-like domain-containing protein 9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0012197
Physical Location & Seq
Forward (+)
24127119 .. 24129188
2070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG234300.1

Sequence Viewer

Length: 546 bp
ATGGCAGGGATTGCGTTTGGTAGTTGCTGTTCAACTATCTCTTCCACCAGCAATTTCCGCACTTCTTGGTTGAAACTTGAGACTCGTACTCTTCGTGGAGGAACCATCTTAGGGAAGCCAAACAGTGTTAGAACTCTGGCAATTAGAGCAGAAGTGAACTTTGTAAGTGCAGACGAAGCTAAAGAACTAATTGCAGAAGGATATACGGTTATTGATGTTCGGGACAAATCTCAATATGATAGAGCTCATATCAAATCATGTTATCATGTGCCTCTTTTCATCGAAAATCAAGACAATGACATCGGCACAATTGTAAAGAGGACTCTGCACAACAATTTCTCGGGTCTGTTCTTCGGGTTACCTTTCACTAAACCCAACCCTGAGTTTGTACAATCTGTTAAAGGCCAGTTTTCACCTCAGAGTAAACTGTTACTGGTGTGCCAGGAAGGATTGAGGTCTGCTGCTGCTGCTAGTAAGTTGGAGCAGGCTGGTTTCCAAGACATAGCATGTATAACATCAGGGCTGCAAAAAGTAAAACCAGGTTAG

Protein Analysis

181

Amino Acids

19.77

Weight (kDa)

9.02

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 56 - 178 3.1e-11 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012197)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42220
fragaria_vesca FvH4_6g22700 FvH4_6g22700 FvH4_6g22700
malus_domestica MD12G1061100.v1.1 MD14G1060800.v1.1
prunus_persica Prupe.7G073600_v2.0.a1 Prupe.7G073600_v2.0.a1
pyrus_communis pycom12g05400
rosa_chinensis RchiOBHm_Chr3g0480311
rosa_laevigata RLG00000023493
rosa_multiflora Rmu_ssc0000353.1_g000001
rosa_roxburghii Rroxscaffold_6G00401570
rosa_rugosa Rorug03G0182400
rosa_samantha Rh3AG234300 Rh3BG266600 Rh3CG264100 Rh3DG259600
rosa_wichuraiana Rw3G021030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 58
AfaI GTAC 2 cut(s) 88, 390
AfiI CCNNNNNNNGG 1 cut(s) 111
AgsI TTSAA 2 cut(s) 33, 73
AjnI CCWGG 2 cut(s) 441, 538
AluBI AGCT 2 cut(s) 179, 245
AluI AGCT 2 cut(s) 179, 245
Alw21I GWGCWC 1 cut(s) 247
Alw26I GTCTC 1 cut(s) 74
Ama87I CYCGRG 1 cut(s) 340
AoxI GGCC 1 cut(s) 403
ApeKI GCWGC 4 cut(s) 461, 464, 467, 523
AsuHPI GGTGA 1 cut(s) 405
AvaI CYCGRG 1 cut(s) 340
BanII GRGCYC 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 247
BbvI GCAGC 4 cut(s) 448, 451, 454, 510
BccI CCATC 1 cut(s) 113
BciT130I CCWGG 2 cut(s) 443, 540
BcoDI GTCTC 1 cut(s) 74
BfaI CTAG 1 cut(s) 471
BisI GCNGC 4 cut(s) 462, 465, 468, 524
BlsI GCNGC 4 cut(s) 463, 466, 469, 525
Bme1390I CCNGG 2 cut(s) 443, 540
BmeT110I CYCGRG 1 cut(s) 340
BmiI GGNNCC 1 cut(s) 103
BmrFI CCNGG 2 cut(s) 443, 540
BpuEI CTTGAG 1 cut(s) 98
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse1I ACTGG 2 cut(s) 406, 438
BseBI CCWGG 2 cut(s) 443, 540
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 2 cut(s) 372, 431
BseNI ACTGG 2 cut(s) 406, 438
BseXI GCAGC 4 cut(s) 448, 451, 454, 510
BsgI GTGCAG 2 cut(s) 189, 311
BshFI GGCC 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 247
BsiHKCI CYCGRG 1 cut(s) 340
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 74
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 1 cut(s) 405
BsoBI CYCGRG 1 cut(s) 340
Bsp1286I GDGCHC 1 cut(s) 247
Bsp1407I TGTACA 1 cut(s) 388
BspACI CCGC 1 cut(s) 58
BspANI GGCC 1 cut(s) 405
BspCNI CTCAG 2 cut(s) 373, 430
BspLI GGNNCC 1 cut(s) 103
BsrGI TGTACA 1 cut(s) 388
BsrI ACTGG 2 cut(s) 406, 438
Bst2UI CCWGG 2 cut(s) 443, 540
Bst4CI ACNGT 3 cut(s) 125, 208, 429
Bst6I CTCTTC 2 cut(s) 46, 96
BstAPI GCANNNNNTGC 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 388
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 3 cut(s) 109, 381, 417
BstEII GGTNACC 1 cut(s) 357
BstMAI GTCTC 1 cut(s) 74
BstMWI GCNNNNNNNGC 5 cut(s) 11, 57, 146, 176, 467
BstNI CCWGG 2 cut(s) 443, 540
BstNSI RCATGY 1 cut(s) 510
BstPI GGTNACC 1 cut(s) 357
BstSCI CCNGG 2 cut(s) 441, 538
BstV1I GCAGC 4 cut(s) 448, 451, 454, 510
BsuRI GGCC 1 cut(s) 405
BtsIMutI CAGTG 1 cut(s) 130
Cac8I GCNNGC 1 cut(s) 486
CsiI ACCWGGT 1 cut(s) 538
Csp6I GTAC 2 cut(s) 87, 389
CviAII CATG 3 cut(s) 258, 266, 507
CviJI RGCY 6 cut(s) 118, 179, 245, 405, 488, 523
CviKI_1 RGCY 6 cut(s) 118, 179, 245, 405, 488, 523
CviQI GTAC 2 cut(s) 87, 389
DdeI CTNAG 3 cut(s) 109, 381, 417
Eam1104I CTCTTC 2 cut(s) 46, 96
EarI CTCTTC 2 cut(s) 46, 96
Ecl136II GAGCTC 1 cut(s) 245
Eco24I GRGCYC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 340
Eco91I GGTNACC 1 cut(s) 357
EcoICRI GAGCTC 1 cut(s) 245
EcoO65I GGTNACC 1 cut(s) 357
EcoRII CCWGG 2 cut(s) 441, 538
EcoT38I GRGCYC 1 cut(s) 247
FaeI CATG 3 cut(s) 261, 269, 510
FaiI YATR 8 cut(s) 204, 237, 249, 259, 267, 503, 508, 512
FaqI GGGAC 1 cut(s) 236
FatI CATG 3 cut(s) 257, 265, 506
Fnu4HI GCNGC 4 cut(s) 462, 465, 468, 524
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 4 cut(s) 462, 465, 468, 524
FspBI CTAG 1 cut(s) 471
GluI GCNGC 4 cut(s) 462, 465, 468, 524
HaeIII GGCC 1 cut(s) 405
Hin1II CATG 3 cut(s) 261, 269, 510
HinfI GANTC 2 cut(s) 82, 322
HphI GGTGA 1 cut(s) 405
Hpy166II GTNNAC 2 cut(s) 157, 425
Hpy188I TCNGA 1 cut(s) 420
Hpy188III TCNNGA 2 cut(s) 221, 290
Hpy8I GTNNAC 2 cut(s) 157, 425
HpyAV CCTTC 2 cut(s) 191, 440
HpyCH4III ACNGT 3 cut(s) 125, 208, 429
HpyCH4V TGCA 4 cut(s) 170, 194, 328, 526
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 57, 146, 176, 467
HpyF3I CTNAG 3 cut(s) 109, 381, 417
Hsp92II CATG 3 cut(s) 261, 269, 510
LmnI GCTCC 1 cut(s) 481
Lsp1109I GCAGC 4 cut(s) 448, 451, 454, 510
MabI ACCWGGT 1 cut(s) 538
MaeI CTAG 1 cut(s) 471
MaeIII GTNAC 2 cut(s) 357, 429
MboII GAAGA 3 cut(s) 33, 83, 343
MfeI CAATTG 1 cut(s) 309
MhlI GDGCHC 1 cut(s) 247
MluCI AATT 5 cut(s) 52, 141, 189, 309, 334
MlyI GAGTC 2 cut(s) 76, 316
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 5 cut(s) 92, 282, 312, 426, 447
MseI TTAA 1 cut(s) 399
MspR9I CCNGG 2 cut(s) 443, 540
MunI CAATTG 1 cut(s) 309
MvaI CCWGG 2 cut(s) 443, 540
MwoI GCNNNNNNNGC 5 cut(s) 11, 57, 146, 176, 467
NlaIII CATG 3 cut(s) 261, 269, 510
NlaIV GGNNCC 1 cut(s) 103
NspI RCATGY 1 cut(s) 510
PcsI WCGNNNNNNNCGW 1 cut(s) 91
PkrI GCNGC 4 cut(s) 463, 466, 469, 525
PleI GAGTC 2 cut(s) 76, 316
PpsI GAGTC 2 cut(s) 76, 316
Psp124BI GAGCTC 1 cut(s) 247
Psp6I CCWGG 2 cut(s) 441, 538
PspEI GGTNACC 1 cut(s) 357
PspGI CCWGG 2 cut(s) 441, 538
PspN4I GGNNCC 1 cut(s) 103
PsrI GAACNNNNNNTAC 2 cut(s) 13, 45
RsaI GTAC 2 cut(s) 88, 390
RsaNI GTAC 2 cut(s) 87, 389
SacI GAGCTC 1 cut(s) 247
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 4 cut(s) 462, 465, 468, 524
SchI GAGTC 2 cut(s) 76, 316
ScrFI CCNGG 2 cut(s) 443, 540
SduI GDGCHC 1 cut(s) 247
SetI ASST 6 cut(s) 181, 247, 364, 418, 458, 544
SexAI ACCWGGT 1 cut(s) 538
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
Sse9I AATT 5 cut(s) 52, 141, 189, 309, 334
SsiI CCGC 1 cut(s) 58
SspMI CTAG 1 cut(s) 471
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 2 cut(s) 441, 538
TaaI ACNGT 3 cut(s) 125, 208, 429
TaqI TCGA 1 cut(s) 282
TasI AATT 5 cut(s) 52, 141, 189, 309, 334
TatI WGTACW 1 cut(s) 388
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 1 cut(s) 130
TseI GCWGC 4 cut(s) 461, 464, 467, 523
TspDTI ATGAA 1 cut(s) 268
TspRI CASTG 1 cut(s) 130
XceI RCATGY 1 cut(s) 510
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.