Rroxscaffold_6G00401570

Rhodanese-like domain-containing protein 9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0012197
Physical Location & Seq
Reverse (-)
23952374 .. 23954417
2044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00401570.1

Sequence Viewer

Length: 717 bp
ATGGCAGGGATTGCGTTTGGCACTTGCTGTTCAACTATCTCTTCCACCAGCAATTTCCGCACTTCTTGGTTGAAACTTGAGACCAGTACTCATCGTGGAGGAACCATCTTAAAGAAACCAAACAGTGTTAGAACTCTGGCAATTAGAGCAGAAGTGAACTTTGTAAGTGCAGACGAAGCTAAAGAACTAATTGCAGAAGGATATACGGTTATTGATGTTCGGGACAAATCTCAATATGATCGAGCTCATATCAAATCATGTTATCATGTGCCCCTTTTCATCGAAAATCAAGACAATGACATCGGCACAATTGTAAAGAGGACTGTGCACAACAATTTCTCGGGTCTGTTCTTCGGGTTACCTTTCACTAAACCGAACCCTGAGTTTGTACAAGCTGTTAAAGGCCAGTTTTCACCTCAGAGTAGACTGTTACTGGTGTGCCAGGAAGGATTGAGGTCTGCAGCTGCTGCCAGTAAGTTGGAGCAGGCTGGTTTCCAAGACATAGCATGTATAACATCAGGGCTGCAAAAAGTAAAACCAGGAGCATTTGATGCTGTTGGATCCACTGAGTTGCAAGATGCTGGCAAAGCTGGTTTGATCACAGTACAAGGAAAGATATCAGCTGTACTTGGAACTGTACTAGTCTGTGCATATTTGTTCATCACCTTCTTCCCTGATCAAGCAGAGAAGCTACTTCAATTGGCTCCTACAGGCTAG

Protein Analysis

238

Amino Acids

25.69

Weight (kDa)

8.5

Isoelectric Point (pI)

32.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 56 - 178 1.1e-10 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012197)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42220
fragaria_vesca FvH4_6g22700 FvH4_6g22700 FvH4_6g22700
malus_domestica MD12G1061100.v1.1 MD14G1060800.v1.1
prunus_persica Prupe.7G073600_v2.0.a1 Prupe.7G073600_v2.0.a1
pyrus_communis pycom12g05400
rosa_chinensis RchiOBHm_Chr3g0480311
rosa_laevigata RLG00000023493
rosa_multiflora Rmu_ssc0000353.1_g000001
rosa_roxburghii Rroxscaffold_6G00401570
rosa_rugosa Rorug03G0182400
rosa_samantha Rh3AG234300 Rh3BG266600 Rh3CG264100 Rh3DG259600
rosa_wichuraiana Rw3G021030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 424
AciI CCGC 1 cut(s) 58
AclWI GGATC 2 cut(s) 555, 568
AfaI GTAC 5 cut(s) 88, 390, 606, 627, 639
AgsI TTSAA 3 cut(s) 33, 73, 698
AhlI ACTAGT 1 cut(s) 640
AjnI CCWGG 2 cut(s) 441, 538
AluBI AGCT 7 cut(s) 179, 245, 395, 464, 590, 623, 691
AluI AGCT 7 cut(s) 179, 245, 395, 464, 590, 623, 691
Alw21I GWGCWC 2 cut(s) 247, 330
Alw26I GTCTC 1 cut(s) 74
Alw44I GTGCAC 1 cut(s) 326
AlwI GGATC 2 cut(s) 555, 568
AlwNI CAGNNNCTG 1 cut(s) 467
Ama87I CYCGRG 1 cut(s) 340
AoxI GGCC 1 cut(s) 403
ApaLI GTGCAC 1 cut(s) 326
ApeKI GCWGC 4 cut(s) 461, 464, 467, 523
AsuHPI GGTGA 2 cut(s) 405, 655
AvaI CYCGRG 1 cut(s) 340
BaeGI GKGCMC 2 cut(s) 273, 330
BamHI GGATCC 1 cut(s) 560
BanII GRGCYC 1 cut(s) 247
Bbv12I GWGCWC 2 cut(s) 247, 330
BbvI GCAGC 4 cut(s) 451, 454, 473, 510
BccI CCATC 1 cut(s) 113
BciT130I CCWGG 2 cut(s) 443, 540
BclI TGATCA 2 cut(s) 597, 676
BcoDI GTCTC 1 cut(s) 74
BcuI ACTAGT 1 cut(s) 640
BfaI CTAG 2 cut(s) 641, 715
BfmI CTRYAG 2 cut(s) 459, 708
BisI GCNGC 4 cut(s) 462, 465, 468, 524
BlsI GCNGC 4 cut(s) 463, 466, 469, 525
BmcAI AGTACT 1 cut(s) 88
Bme1390I CCNGG 2 cut(s) 443, 540
BmeT110I CYCGRG 1 cut(s) 340
BmiI GGNNCC 3 cut(s) 103, 562, 705
BmrFI CCNGG 2 cut(s) 443, 540
BmsI GCATC 2 cut(s) 541, 568
BpuEI CTTGAG 1 cut(s) 98
BsaI GGTCTC 1 cut(s) 74
Bse1I ACTGG 4 cut(s) 84, 406, 438, 471
BseBI CCWGG 2 cut(s) 443, 540
BseMII CTCAG 3 cut(s) 372, 431, 558
BseNI ACTGG 4 cut(s) 84, 406, 438, 471
BseSI GKGCMC 2 cut(s) 273, 330
BseXI GCAGC 4 cut(s) 451, 454, 473, 510
BsgI GTGCAG 1 cut(s) 189
BshFI GGCC 1 cut(s) 405
BsiHKAI GWGCWC 2 cut(s) 247, 330
BsiHKCI CYCGRG 1 cut(s) 340
BslFI GGGAC 1 cut(s) 236
BsmAI GTCTC 1 cut(s) 74
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 1 cut(s) 405
Bso31I GGTCTC 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 340
Bsp1286I GDGCHC 3 cut(s) 247, 273, 330
Bsp1407I TGTACA 1 cut(s) 388
Bsp143I GATC 4 cut(s) 238, 560, 597, 676
BspACI CCGC 1 cut(s) 58
BspANI GGCC 1 cut(s) 405
BspCNI CTCAG 3 cut(s) 373, 430, 559
BspLI GGNNCC 3 cut(s) 103, 562, 705
BspMAI CTGCAG 1 cut(s) 463
BspPI GGATC 2 cut(s) 555, 568
BspTNI GGTCTC 1 cut(s) 74
BsrGI TGTACA 1 cut(s) 388
BsrI ACTGG 4 cut(s) 84, 406, 438, 471
BssMI GATC 4 cut(s) 238, 560, 597, 676
Bst2UI CCWGG 2 cut(s) 443, 540
Bst4CI ACNGT 6 cut(s) 125, 208, 325, 429, 604, 637
Bst6I CTCTTC 1 cut(s) 46
BstAPI GCANNNNNTGC 3 cut(s) 11, 467, 551
BstAUI TGTACA 1 cut(s) 388
BstC8I GCNNGC 2 cut(s) 486, 583
BstDEI CTNAG 3 cut(s) 381, 417, 567
BstEII GGTNACC 1 cut(s) 357
BstKTI GATC 4 cut(s) 241, 563, 600, 679
BstMAI GTCTC 1 cut(s) 74
BstMBI GATC 4 cut(s) 238, 560, 597, 676
BstMWI GCNNNNNNNGC 7 cut(s) 11, 57, 146, 176, 467, 551, 587
BstNI CCWGG 2 cut(s) 443, 540
BstNSI RCATGY 1 cut(s) 510
BstPI GGTNACC 1 cut(s) 357
BstSCI CCNGG 2 cut(s) 441, 538
BstSFI CTRYAG 2 cut(s) 459, 708
BstSLI GKGCMC 2 cut(s) 273, 330
BstV1I GCAGC 4 cut(s) 451, 454, 473, 510
BstX2I RGATCY 1 cut(s) 560
BstXI CCANNNNNNTGG 1 cut(s) 478
BstYI RGATCY 1 cut(s) 560
BsuRI GGCC 1 cut(s) 405
BtsIMutI CAGTG 2 cut(s) 130, 564
Cac8I GCNNGC 2 cut(s) 486, 583
CaiI CAGNNNCTG 1 cut(s) 467
Csp6I GTAC 5 cut(s) 87, 389, 605, 626, 638
CviAII CATG 3 cut(s) 258, 266, 507
CviQI GTAC 5 cut(s) 87, 389, 605, 626, 638
DdeI CTNAG 3 cut(s) 381, 417, 567
DpnI GATC 4 cut(s) 240, 562, 599, 678
DpnII GATC 4 cut(s) 238, 560, 597, 676
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
Ecl136II GAGCTC 1 cut(s) 245
Eco24I GRGCYC 1 cut(s) 247
Eco31I GGTCTC 1 cut(s) 74
Eco32I GATATC 1 cut(s) 618
Eco53kI GAGCTC 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 340
Eco91I GGTNACC 1 cut(s) 357
EcoICRI GAGCTC 1 cut(s) 245
EcoO65I GGTNACC 1 cut(s) 357
EcoRII CCWGG 2 cut(s) 441, 538
EcoRV GATATC 1 cut(s) 618
EcoT38I GRGCYC 1 cut(s) 247
FaeI CATG 3 cut(s) 261, 269, 510
FaiI YATR 9 cut(s) 204, 237, 249, 259, 267, 503, 508, 512, 652
FaqI GGGAC 1 cut(s) 236
FatI CATG 3 cut(s) 257, 265, 506
FbaI TGATCA 2 cut(s) 597, 676
FblI GTMKAC 1 cut(s) 424
Fnu4HI GCNGC 4 cut(s) 462, 465, 468, 524
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 4 cut(s) 462, 465, 468, 524
FspBI CTAG 2 cut(s) 641, 715
GluI GCNGC 4 cut(s) 462, 465, 468, 524
HaeIII GGCC 1 cut(s) 405
Hin1II CATG 3 cut(s) 261, 269, 510
HphI GGTGA 2 cut(s) 405, 655
Hpy166II GTNNAC 3 cut(s) 157, 328, 425
Hpy188I TCNGA 1 cut(s) 420
Hpy188III TCNNGA 2 cut(s) 221, 290
Hpy8I GTNNAC 3 cut(s) 157, 328, 425
HpyAV CCTTC 3 cut(s) 191, 440, 676
HpyCH4III ACNGT 6 cut(s) 125, 208, 325, 429, 604, 637
HpyCH4V TGCA 7 cut(s) 170, 194, 328, 461, 526, 574, 650
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 57, 146, 176, 467, 551, 587
HpyF3I CTNAG 3 cut(s) 381, 417, 567
Hsp92II CATG 3 cut(s) 261, 269, 510
Ksp22I TGATCA 2 cut(s) 597, 676
Kzo9I GATC 4 cut(s) 238, 560, 597, 676
LmnI GCTCC 3 cut(s) 481, 542, 709
Lsp1109I GCAGC 4 cut(s) 451, 454, 473, 510
LweI GCATC 2 cut(s) 541, 568
MaeI CTAG 2 cut(s) 641, 715
MaeIII GTNAC 2 cut(s) 357, 429
MalI GATC 4 cut(s) 240, 562, 599, 678
MboI GATC 4 cut(s) 238, 560, 597, 676
MboII GAAGA 3 cut(s) 33, 343, 661
MfeI CAATTG 2 cut(s) 309, 698
MflI RGATCY 1 cut(s) 560
MhlI GDGCHC 3 cut(s) 247, 273, 330
MluCI AATT 6 cut(s) 52, 141, 189, 309, 334, 698
MmeI TCCRAC 2 cut(s) 459, 538
MnlI CCTC 4 cut(s) 92, 312, 426, 447
MseI TTAA 2 cut(s) 110, 399
MspA1I CMGCKG 2 cut(s) 464, 623
MspR9I CCNGG 2 cut(s) 443, 540
MunI CAATTG 2 cut(s) 309, 698
MvaI CCWGG 2 cut(s) 443, 540
MwoI GCNNNNNNNGC 7 cut(s) 11, 57, 146, 176, 467, 551, 587
NdeII GATC 4 cut(s) 238, 560, 597, 676
NlaIII CATG 3 cut(s) 261, 269, 510
NlaIV GGNNCC 3 cut(s) 103, 562, 705
NspI RCATGY 1 cut(s) 510
PkrI GCNGC 4 cut(s) 463, 466, 469, 525
Psp124BI GAGCTC 1 cut(s) 247
Psp6I CCWGG 2 cut(s) 441, 538
PspEI GGTNACC 1 cut(s) 357
PspGI CCWGG 2 cut(s) 441, 538
PspN4I GGNNCC 3 cut(s) 103, 562, 705
PstI CTGCAG 1 cut(s) 463
PstNI CAGNNNCTG 1 cut(s) 467
PsuI RGATCY 1 cut(s) 560
PvuII CAGCTG 2 cut(s) 464, 623
RsaI GTAC 5 cut(s) 88, 390, 606, 627, 639
RsaNI GTAC 5 cut(s) 87, 389, 605, 626, 638
SacI GAGCTC 1 cut(s) 247
SaqAI TTAA 2 cut(s) 110, 399
SatI GCNGC 4 cut(s) 462, 465, 468, 524
Sau3AI GATC 4 cut(s) 238, 560, 597, 676
ScaI AGTACT 1 cut(s) 88
ScrFI CCNGG 2 cut(s) 443, 540
SduI GDGCHC 3 cut(s) 247, 273, 330
SfaNI GCATC 2 cut(s) 541, 568
SfcI CTRYAG 2 cut(s) 459, 708
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
SpeI ACTAGT 1 cut(s) 640
Sse9I AATT 6 cut(s) 52, 141, 189, 309, 334, 698
SsiI CCGC 1 cut(s) 58
SspMI CTAG 2 cut(s) 641, 715
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 2 cut(s) 441, 538
TaaI ACNGT 6 cut(s) 125, 208, 325, 429, 604, 637
TaqI TCGA 2 cut(s) 241, 282
TasI AATT 6 cut(s) 52, 141, 189, 309, 334, 698
TatI WGTACW 5 cut(s) 86, 388, 604, 625, 637
Tru1I TTAA 2 cut(s) 110, 399
Tru9I TTAA 2 cut(s) 110, 399
TscAI CASTG 2 cut(s) 130, 571
TseI GCWGC 4 cut(s) 461, 464, 467, 523
TspDTI ATGAA 2 cut(s) 268, 649
TspRI CASTG 2 cut(s) 130, 571
VneI GTGCAC 1 cut(s) 326
XceI RCATGY 1 cut(s) 510
XmiI GTMKAC 1 cut(s) 424
XspI CTAG 2 cut(s) 641, 715
ZrmI AGTACT 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.