FvH4_6g22700

Rhodanese-like domain-containing protein 9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012197
Physical Location & Seq
Forward (+)
16321608 .. 16323801
2194 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g22700.t1

Sequence Viewer

Length: 717 bp
ATGGCAGGGATTGCGTTTGGCACTTGCTGTTCAACTATCTCTTCCACCAGCAATTTCCGCACTTCTTGGTTGAAACTTGAGACTCGTTCTCATCGAGGAGGAACCATCTTAGGGAAATCAAACAATGCCAGGACTTTGGCAATTAGAGCACAAGTGAATTTTGTGAATGCAGACGAAGCTAAAGAATTAATTGCAGAGGGATATACGGTTATTGATGTTCGGGACAAATCTCAATATGATCGAGCTCATATCAAATCATGTTATCATGTGCCTCTTTTCATCAAAAACGAAGACAACGACATCGGCACAATTGTAAAGAGGACTGTGCACAACAATTTCTCGGGTCTGTTCTTCGGGATACCTTTCACTAAACCCAACCCAGAATTTGTACAATCTGTGAAGGGCCAGTTTTCACCTCAGAGTAAACTGTTACTGGTGTGCCAGGAAGGATTGAGATCAGCTGTTGCTGCTAGTAAGTTGGAGCAGGCTGGTTTCCAAGAGATAGCATGTATAACATCAGGGCTGCAAAAAGTAAAACCAGGAGTATTTGATTCTGTTGGATCCACTCAGTTGCAAGATGCTGGCAAAGCTGGTTTGGTAACAGTACAAGGAAAGATCTCAGCTGTACTTGGAACCGTGCTAGTCTGTGCGTATTTGTTCATCACCTTCTTCCCTGATCAAGCAGAGAAGCTACTTCAATTGGCGCCTACAGGCTAG

Protein Analysis

239

Amino Acids

25.75

Weight (kDa)

9.03

Isoelectric Point (pI)

32.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 57 - 178 8.3e-10 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012197)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42220
fragaria_vesca FvH4_6g22700 FvH4_6g22700 FvH4_6g22700
malus_domestica MD12G1061100.v1.1 MD14G1060800.v1.1
prunus_persica Prupe.7G073600_v2.0.a1 Prupe.7G073600_v2.0.a1
pyrus_communis pycom12g05400
rosa_chinensis RchiOBHm_Chr3g0480311
rosa_laevigata RLG00000023493
rosa_multiflora Rmu_ssc0000353.1_g000001
rosa_roxburghii Rroxscaffold_6G00401570
rosa_rugosa Rorug03G0182400
rosa_samantha Rh3AG234300 Rh3BG266600 Rh3CG264100 Rh3DG259600
rosa_wichuraiana Rw3G021030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 703
AciI CCGC 1 cut(s) 58
AclWI GGATC 2 cut(s) 555, 568
AcsI RAATTY 2 cut(s) 157, 383
AcyI GRCGYC 1 cut(s) 704
AfaI GTAC 3 cut(s) 390, 606, 627
AfiI CCNNNNNNNGG 1 cut(s) 111
AgsI TTSAA 3 cut(s) 33, 73, 698
AjnI CCWGG 3 cut(s) 128, 441, 538
AluBI AGCT 6 cut(s) 179, 245, 461, 590, 623, 691
AluI AGCT 6 cut(s) 179, 245, 461, 590, 623, 691
Alw21I GWGCWC 3 cut(s) 151, 247, 330
Alw26I GTCTC 1 cut(s) 74
Alw44I GTGCAC 1 cut(s) 326
AlwI GGATC 2 cut(s) 555, 568
Ama87I CYCGRG 1 cut(s) 340
AoxI GGCC 1 cut(s) 403
ApaLI GTGCAC 1 cut(s) 326
ApeKI GCWGC 2 cut(s) 467, 523
ApoI RAATTY 2 cut(s) 157, 383
AseI ATTAAT 1 cut(s) 188
AspLEI GCGC 1 cut(s) 706
AspS9I GGNCC 1 cut(s) 403
AsuHPI GGTGA 2 cut(s) 405, 655
AvaI CYCGRG 1 cut(s) 340
BaeGI GKGCMC 1 cut(s) 330
BamHI GGATCC 1 cut(s) 560
BanI GGYRCC 1 cut(s) 703
BanII GRGCYC 1 cut(s) 247
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 3 cut(s) 151, 247, 330
BbvI GCAGC 2 cut(s) 454, 510
BccI CCATC 1 cut(s) 113
BciT130I CCWGG 3 cut(s) 130, 443, 540
BciVI GTATCC 1 cut(s) 351
BclI TGATCA 1 cut(s) 676
BcoDI GTCTC 1 cut(s) 74
BfaI CTAG 3 cut(s) 471, 641, 715
BfmI CTRYAG 1 cut(s) 708
BfoI RGCGCY 1 cut(s) 707
BfuI GTATCC 1 cut(s) 351
BglII AGATCT 1 cut(s) 615
BisI GCNGC 2 cut(s) 468, 524
BlsI GCNGC 2 cut(s) 469, 525
Bme1390I CCNGG 3 cut(s) 130, 443, 540
BmeT110I CYCGRG 1 cut(s) 340
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 4 cut(s) 103, 562, 634, 705
BmrFI CCNGG 3 cut(s) 130, 443, 540
BmsI GCATC 1 cut(s) 568
BpiI GAAGAC 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 454
BsaHI GRCGYC 1 cut(s) 704
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse1I ACTGG 2 cut(s) 406, 438
Bse8I GATNNNNATC 1 cut(s) 454
BseBI CCWGG 3 cut(s) 130, 443, 540
BseJI GATNNNNATC 1 cut(s) 454
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 3 cut(s) 431, 581, 633
BseNI ACTGG 2 cut(s) 406, 438
BseRI GAGGAG 1 cut(s) 111
BseSI GKGCMC 1 cut(s) 330
BseXI GCAGC 2 cut(s) 454, 510
BshFI GGCC 1 cut(s) 405
BshNI GGYRCC 1 cut(s) 703
BsiHKAI GWGCWC 3 cut(s) 151, 247, 330
BsiHKCI CYCGRG 1 cut(s) 340
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 74
BsmFI GGGAC 1 cut(s) 236
BsmI GAATGC 1 cut(s) 172
BsnI GGCC 1 cut(s) 405
BsoBI CYCGRG 1 cut(s) 340
Bsp1286I GDGCHC 3 cut(s) 151, 247, 330
Bsp1407I TGTACA 1 cut(s) 388
Bsp143I GATC 5 cut(s) 238, 455, 560, 615, 676
BspACI CCGC 1 cut(s) 58
BspANI GGCC 1 cut(s) 405
BspCNI CTCAG 3 cut(s) 430, 580, 632
BspLI GGNNCC 4 cut(s) 103, 562, 634, 705
BspPI GGATC 2 cut(s) 555, 568
BspT107I GGYRCC 1 cut(s) 703
BsrGI TGTACA 1 cut(s) 388
BsrI ACTGG 2 cut(s) 406, 438
BssMI GATC 5 cut(s) 238, 455, 560, 615, 676
BssNI GRCGYC 1 cut(s) 704
Bst2UI CCWGG 3 cut(s) 130, 443, 540
Bst4CI ACNGT 5 cut(s) 208, 325, 429, 604, 637
Bst6I CTCTTC 1 cut(s) 46
BstACI GRCGYC 1 cut(s) 704
BstAPI GCANNNNNTGC 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 388
BstC8I GCNNGC 2 cut(s) 486, 583
BstDEI CTNAG 4 cut(s) 109, 417, 567, 619
BstH2I RGCGCY 1 cut(s) 707
BstHHI GCGC 1 cut(s) 706
BstKTI GATC 5 cut(s) 241, 458, 563, 618, 679
BstMAI GTCTC 1 cut(s) 74
BstMBI GATC 5 cut(s) 238, 455, 560, 615, 676
BstMWI GCNNNNNNNGC 6 cut(s) 11, 57, 146, 176, 467, 587
BstNI CCWGG 3 cut(s) 130, 443, 540
BstNSI RCATGY 1 cut(s) 510
BstSCI CCNGG 3 cut(s) 128, 441, 538
BstSFI CTRYAG 1 cut(s) 708
BstSLI GKGCMC 1 cut(s) 330
BstV1I GCAGC 2 cut(s) 454, 510
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 2 cut(s) 560, 615
BstXI CCANNNNNNTGG 1 cut(s) 136
BstYI RGATCY 2 cut(s) 560, 615
BsuI GTATCC 1 cut(s) 351
BsuRI GGCC 1 cut(s) 405
Cac8I GCNNGC 2 cut(s) 486, 583
CfoI GCGC 1 cut(s) 706
Cfr13I GGNCC 1 cut(s) 403
Csp6I GTAC 3 cut(s) 389, 605, 626
CviAII CATG 3 cut(s) 258, 266, 507
CviQI GTAC 3 cut(s) 389, 605, 626
DdeI CTNAG 4 cut(s) 109, 417, 567, 619
DinI GGCGCC 1 cut(s) 705
DpnI GATC 5 cut(s) 240, 457, 562, 617, 678
DpnII GATC 5 cut(s) 238, 455, 560, 615, 676
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
Ecl136II GAGCTC 1 cut(s) 245
Eco24I GRGCYC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 340
EcoICRI GAGCTC 1 cut(s) 245
EcoRII CCWGG 3 cut(s) 128, 441, 538
EcoT38I GRGCYC 1 cut(s) 247
EgeI GGCGCC 1 cut(s) 705
EheI GGCGCC 1 cut(s) 705
FaeI CATG 3 cut(s) 261, 269, 510
FaiI YATR 7 cut(s) 204, 237, 249, 259, 267, 508, 512
FaqI GGGAC 1 cut(s) 236
FatI CATG 3 cut(s) 257, 265, 506
FbaI TGATCA 1 cut(s) 676
Fnu4HI GCNGC 2 cut(s) 468, 524
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 2 cut(s) 468, 524
FspBI CTAG 3 cut(s) 471, 641, 715
GlaI GCGC 1 cut(s) 705
GluI GCNGC 2 cut(s) 468, 524
HaeII RGCGCY 1 cut(s) 707
HaeIII GGCC 1 cut(s) 405
HhaI GCGC 1 cut(s) 706
Hin1I GRCGYC 1 cut(s) 704
Hin1II CATG 3 cut(s) 261, 269, 510
Hin6I GCGC 1 cut(s) 704
HinP1I GCGC 1 cut(s) 704
HinfI GANTC 2 cut(s) 82, 551
HphI GGTGA 2 cut(s) 405, 655
Hpy166II GTNNAC 2 cut(s) 328, 425
Hpy188I TCNGA 1 cut(s) 420
Hpy188III TCNNGA 2 cut(s) 221, 355
Hpy8I GTNNAC 2 cut(s) 328, 425
HpyAV CCTTC 3 cut(s) 394, 440, 676
HpyCH4III ACNGT 5 cut(s) 208, 325, 429, 604, 637
HpyCH4V TGCA 5 cut(s) 170, 194, 328, 526, 574
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 57, 146, 176, 467, 587
HpyF3I CTNAG 4 cut(s) 109, 417, 567, 619
Hsp92I GRCGYC 1 cut(s) 704
Hsp92II CATG 3 cut(s) 261, 269, 510
HspAI GCGC 1 cut(s) 704
KasI GGCGCC 1 cut(s) 703
Ksp22I TGATCA 1 cut(s) 676
Kzo9I GATC 5 cut(s) 238, 455, 560, 615, 676
LmnI GCTCC 1 cut(s) 481
Lsp1109I GCAGC 2 cut(s) 454, 510
LweI GCATC 1 cut(s) 568
MaeI CTAG 3 cut(s) 471, 641, 715
MaeIII GTNAC 2 cut(s) 429, 598
MalI GATC 5 cut(s) 240, 457, 562, 617, 678
MboI GATC 5 cut(s) 238, 455, 560, 615, 676
MboII GAAGA 4 cut(s) 33, 302, 343, 661
MfeI CAATTG 2 cut(s) 309, 698
MflI RGATCY 2 cut(s) 560, 615
MhlI GDGCHC 3 cut(s) 151, 247, 330
MluCI AATT 9 cut(s) 52, 141, 157, 185, 189, 309, 334, 383, 698
Mly113I GGCGCC 1 cut(s) 704
MlyI GAGTC 1 cut(s) 76
MmeI TCCRAC 2 cut(s) 459, 538
MnlI CCTC 6 cut(s) 89, 92, 190, 282, 312, 426
MseI TTAA 1 cut(s) 188
MspA1I CMGCKG 2 cut(s) 461, 623
MspR9I CCNGG 3 cut(s) 130, 443, 540
MunI CAATTG 2 cut(s) 309, 698
Mva1269I GAATGC 1 cut(s) 172
MvaI CCWGG 3 cut(s) 130, 443, 540
MwoI GCNNNNNNNGC 6 cut(s) 11, 57, 146, 176, 467, 587
NarI GGCGCC 1 cut(s) 704
NdeII GATC 5 cut(s) 238, 455, 560, 615, 676
NlaIII CATG 3 cut(s) 261, 269, 510
NlaIV GGNNCC 4 cut(s) 103, 562, 634, 705
NspI RCATGY 1 cut(s) 510
PcsI WCGNNNNNNNCGW 2 cut(s) 91, 294
PctI GAATGC 1 cut(s) 172
PfeI GAWTC 1 cut(s) 551
PkrI GCNGC 2 cut(s) 469, 525
PleI GAGTC 1 cut(s) 76
PluTI GGCGCC 1 cut(s) 707
PpsI GAGTC 1 cut(s) 76
PshBI ATTAAT 1 cut(s) 188
Psp124BI GAGCTC 1 cut(s) 247
Psp6I CCWGG 3 cut(s) 128, 441, 538
PspGI CCWGG 3 cut(s) 128, 441, 538
PspN4I GGNNCC 4 cut(s) 103, 562, 634, 705
PspPI GGNCC 1 cut(s) 403
PsuI RGATCY 2 cut(s) 560, 615
PvuII CAGCTG 2 cut(s) 461, 623
RsaI GTAC 3 cut(s) 390, 606, 627
RsaNI GTAC 3 cut(s) 389, 605, 626
SacI GAGCTC 1 cut(s) 247
SaqAI TTAA 1 cut(s) 188
SatI GCNGC 2 cut(s) 468, 524
Sau3AI GATC 5 cut(s) 238, 455, 560, 615, 676
Sau96I GGNCC 1 cut(s) 403
SchI GAGTC 1 cut(s) 76
ScrFI CCNGG 3 cut(s) 130, 443, 540
SduI GDGCHC 3 cut(s) 151, 247, 330
SetI ASST 9 cut(s) 181, 247, 364, 418, 463, 592, 625, 668, 693
SfaNI GCATC 1 cut(s) 568
SfcI CTRYAG 1 cut(s) 708
SfoI GGCGCC 1 cut(s) 705
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
Sse9I AATT 9 cut(s) 52, 141, 157, 185, 189, 309, 334, 383, 698
SsiI CCGC 1 cut(s) 58
SspDI GGCGCC 1 cut(s) 703
SspMI CTAG 3 cut(s) 471, 641, 715
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 3 cut(s) 128, 441, 538
TaaI ACNGT 5 cut(s) 208, 325, 429, 604, 637
TaqI TCGA 2 cut(s) 94, 241
TasI AATT 9 cut(s) 52, 141, 157, 185, 189, 309, 334, 383, 698
TatI WGTACW 3 cut(s) 388, 604, 625
TfiI GAWTC 1 cut(s) 551
Tru1I TTAA 1 cut(s) 188
Tru9I TTAA 1 cut(s) 188
TseI GCWGC 2 cut(s) 467, 523
TspDTI ATGAA 2 cut(s) 268, 649
VneI GTGCAC 1 cut(s) 326
VspI ATTAAT 1 cut(s) 188
XapI RAATTY 2 cut(s) 157, 383
XceI RCATGY 1 cut(s) 510
XspI CTAG 3 cut(s) 471, 641, 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.