MD14G1060800.v1.1

Rhodanese-like domain-containing protein 9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012197
Physical Location & Seq
Forward (+)
6417639 .. 6420203
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1060800.v1.1.491

Sequence Viewer

Length: 717 bp
ATGGCAGGGATTGGCTCTTGCTCTACGACTCTCTCCTCCACAAGCAATTTCTGCACATCTTGGTTGAAACTTGGCACCCGTACTCATGGTGGAGGAACCATCTTAGGGCAACCAACCCGCCATCGACAAAAATTCTCAATCAGAGCAGAAGTGAATTTTGTAAGTGCAGAAGAAGCAAAAGAACTTGTTGCAGTTGAGGGATATACAGTCATTGATGTCCGGGACAAATCTCAGTATGAACGAGCTCATATCAAATCTTGCCATCATGTGCCCCTCTTCATTGAAAACCAAGACAATGATTTTGGAACAATCATAAAGAGGACTCTCCACAACAATTTTTCTGGTCTCTTCTATGGATTGCCTTTTACTAAACCTAACCCTGAATTTGTAGAGTCAGTTAAAACCAAGTTCTCACCCGAAAGTAAATTGTTACTGGTGTGTCAGGAAGGGCTGAGGTCTGCTGCTGCTGCCAATAAGTTGGAGCAAGTAGGTTTTGAGAACATAGCGTGTATAACATCAGGTCTTCAAAGCGTAAAACCCGGAGAATTTGATTCAGCTGGTTCGACGGAGTTGCAAGATGCCGGCAAAGCCGGATTAGTCACTGTTCAGGGAAAGATCTCAGCTGTGCTTGGAACAGTACTAATCTGTGCATATTTGTTCATCACCTTCTTTCCCGATCAAGCAGAGAAGATACTCGCAATTTTCGCGGCCAGCTAG

Protein Analysis

239

Amino Acids

25.78

Weight (kDa)

7.0

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 55 - 177 2.8e-11 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0012197)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42220
fragaria_vesca FvH4_6g22700 FvH4_6g22700 FvH4_6g22700
malus_domestica MD12G1061100.v1.1 MD14G1060800.v1.1
prunus_persica Prupe.7G073600_v2.0.a1 Prupe.7G073600_v2.0.a1
pyrus_communis pycom12g05400
rosa_chinensis RchiOBHm_Chr3g0480311
rosa_laevigata RLG00000023493
rosa_multiflora Rmu_ssc0000353.1_g000001
rosa_roxburghii Rroxscaffold_6G00401570
rosa_rugosa Rorug03G0182400
rosa_samantha Rh3AG234300 Rh3BG266600 Rh3CG264100 Rh3DG259600
rosa_wichuraiana Rw3G021030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 74
AccII CGCG 1 cut(s) 707
AciI CCGC 2 cut(s) 118, 707
AcoI YGGCCR 1 cut(s) 708
AcsI RAATTY 4 cut(s) 131, 154, 383, 545
AfaI GTAC 2 cut(s) 82, 639
AfiI CCNNNNNNNGG 1 cut(s) 105
AgsI TTSAA 3 cut(s) 67, 284, 527
AluBI AGCT 4 cut(s) 245, 557, 623, 714
AluI AGCT 4 cut(s) 245, 557, 623, 714
Alw21I GWGCWC 1 cut(s) 247
Alw26I GTCTC 1 cut(s) 350
AoxI GGCC 1 cut(s) 708
ApeKI GCWGC 3 cut(s) 461, 464, 467
ApoI RAATTY 4 cut(s) 131, 154, 383, 545
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
AsuC2I CCSGG 2 cut(s) 221, 540
AsuHPI GGTGA 2 cut(s) 405, 655
BaeGI GKGCMC 1 cut(s) 273
BanI GGYRCC 1 cut(s) 74
BanII GRGCYC 1 cut(s) 247
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 1 cut(s) 247
BbvCI CCTCAGC 1 cut(s) 452
BbvI GCAGC 3 cut(s) 448, 451, 454
BccI CCATC 3 cut(s) 107, 129, 270
BcgI CGANNNNNNTGC 2 cut(s) 553, 587
BcnI CCSGG 2 cut(s) 221, 540
BcoDI GTCTC 1 cut(s) 350
BfaI CTAG 1 cut(s) 715
BglII AGATCT 1 cut(s) 615
BisI GCNGC 4 cut(s) 462, 465, 468, 708
BlsI GCNGC 4 cut(s) 463, 466, 469, 709
BmcAI AGTACT 1 cut(s) 639
Bme1390I CCNGG 2 cut(s) 221, 540
BmiI GGNNCC 2 cut(s) 76, 97
BmrFI CCNGG 2 cut(s) 221, 540
BmsI GCATC 1 cut(s) 568
BpiI GAAGAC 1 cut(s) 515
Bpu10I CCTNAGC 1 cut(s) 452
BpuMI CCSGG 2 cut(s) 221, 540
BsaI GGTCTC 1 cut(s) 350
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse118I RCCGGY 1 cut(s) 581
Bse1I ACTGG 1 cut(s) 438
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMII CTCAG 3 cut(s) 245, 443, 633
BseNI ACTGG 1 cut(s) 438
BseRI GAGGAG 1 cut(s) 25
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 3 cut(s) 448, 451, 454
BsgI GTGCAG 2 cut(s) 37, 186
Bsh1236I CGCG 1 cut(s) 707
BshFI GGCC 1 cut(s) 710
BshNI GGYRCC 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 247
BsiSI CCGG 4 cut(s) 220, 540, 582, 591
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 1 cut(s) 105
BsmAI GTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 1 cut(s) 710
Bso31I GGTCTC 1 cut(s) 350
Bsp1286I GDGCHC 2 cut(s) 247, 273
Bsp143I GATC 2 cut(s) 615, 676
BspACI CCGC 2 cut(s) 118, 707
BspANI GGCC 1 cut(s) 710
BspCNI CTCAG 3 cut(s) 244, 444, 632
BspFNI CGCG 1 cut(s) 707
BspLI GGNNCC 2 cut(s) 76, 97
BspT107I GGYRCC 1 cut(s) 74
BspTNI GGTCTC 1 cut(s) 350
BsrFI RCCGGY 1 cut(s) 581
BsrI ACTGG 1 cut(s) 438
BssAI RCCGGY 1 cut(s) 581
BssMI GATC 2 cut(s) 615, 676
Bst4CI ACNGT 3 cut(s) 208, 604, 637
Bst6I CTCTTC 2 cut(s) 281, 353
BstAPI GCANNNNNTGC 1 cut(s) 51
BstC8I GCNNGC 2 cut(s) 583, 712
BstDEI CTNAG 4 cut(s) 103, 231, 452, 619
BstFNI CGCG 1 cut(s) 707
BstKTI GATC 2 cut(s) 618, 679
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 2 cut(s) 615, 676
BstMWI GCNNNNNNNGC 5 cut(s) 51, 173, 467, 587, 704
BstSCI CCNGG 2 cut(s) 219, 538
BstSLI GKGCMC 1 cut(s) 273
BstUI CGCG 1 cut(s) 707
BstV1I GCAGC 3 cut(s) 448, 451, 454
BstV2I GAAGAC 1 cut(s) 515
BstX2I RGATCY 1 cut(s) 615
BstXI CCANNNNNNTGG 1 cut(s) 478
BstYI RGATCY 1 cut(s) 615
BsuRI GGCC 1 cut(s) 710
BtsIMutI CAGTG 1 cut(s) 600
Cac8I GCNNGC 2 cut(s) 583, 712
Cfr10I RCCGGY 1 cut(s) 581
Csp6I GTAC 2 cut(s) 81, 638
CviAII CATG 2 cut(s) 86, 266
CviJI RGCY 8 cut(s) 15, 245, 451, 557, 590, 623, 710, 714
CviKI_1 RGCY 8 cut(s) 15, 245, 451, 557, 590, 623, 710, 714
CviQI GTAC 2 cut(s) 81, 638
DdeI CTNAG 4 cut(s) 103, 231, 452, 619
DpnI GATC 2 cut(s) 617, 678
DpnII GATC 2 cut(s) 615, 676
EaeI YGGCCR 1 cut(s) 708
Eam1104I CTCTTC 2 cut(s) 281, 353
EarI CTCTTC 2 cut(s) 281, 353
Ecl136II GAGCTC 1 cut(s) 245
Eco24I GRGCYC 1 cut(s) 247
Eco31I GGTCTC 1 cut(s) 350
Eco53kI GAGCTC 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 245
EcoT38I GRGCYC 1 cut(s) 247
FaeI CATG 2 cut(s) 89, 269
FaqI GGGAC 1 cut(s) 236
FatI CATG 2 cut(s) 85, 265
FauI CCCGC 1 cut(s) 125
Fnu4HI GCNGC 4 cut(s) 462, 465, 468, 708
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 4 cut(s) 462, 465, 468, 708
FspBI CTAG 1 cut(s) 715
GluI GCNGC 4 cut(s) 462, 465, 468, 708
HaeIII GGCC 1 cut(s) 710
HapII CCGG 4 cut(s) 220, 540, 582, 591
Hin1II CATG 2 cut(s) 89, 269
HinfI GANTC 4 cut(s) 28, 322, 392, 551
HpaII CCGG 4 cut(s) 220, 540, 582, 591
HphI GGTGA 2 cut(s) 405, 655
Hpy188I TCNGA 1 cut(s) 143
Hpy188III TCNNGA 2 cut(s) 443, 674
Hpy99I CGWCG 1 cut(s) 568
HpyAV CCTTC 2 cut(s) 440, 676
HpyCH4III ACNGT 3 cut(s) 208, 604, 637
HpyCH4V TGCA 5 cut(s) 54, 167, 191, 574, 650
HpyF10VI GCNNNNNNNGC 5 cut(s) 51, 173, 467, 587, 704
HpyF3I CTNAG 4 cut(s) 103, 231, 452, 619
Hsp92II CATG 2 cut(s) 89, 269
KroI GCCGGC 1 cut(s) 581
KroNI GCCGGC 1 cut(s) 583
Kzo9I GATC 2 cut(s) 615, 676
LmnI GCTCC 1 cut(s) 481
Lsp1109I GCAGC 3 cut(s) 448, 451, 454
LweI GCATC 1 cut(s) 568
MaeI CTAG 1 cut(s) 715
MaeIII GTNAC 2 cut(s) 429, 598
MalI GATC 2 cut(s) 617, 678
MboI GATC 2 cut(s) 615, 676
MboII GAAGA 5 cut(s) 182, 268, 340, 515, 700
MflI RGATCY 1 cut(s) 615
MhlI GDGCHC 2 cut(s) 247, 273
MluCI AATT 8 cut(s) 46, 131, 154, 334, 383, 425, 545, 699
MlyI GAGTC 3 cut(s) 22, 316, 401
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 6 cut(s) 46, 86, 190, 284, 312, 447
MroNI GCCGGC 1 cut(s) 581
MseI TTAA 1 cut(s) 399
MspA1I CMGCKG 2 cut(s) 557, 623
MspI CCGG 4 cut(s) 220, 540, 582, 591
MspR9I CCNGG 2 cut(s) 221, 540
MvnI CGCG 1 cut(s) 707
MwoI GCNNNNNNNGC 5 cut(s) 51, 173, 467, 587, 704
NaeI GCCGGC 1 cut(s) 583
NciI CCSGG 2 cut(s) 221, 540
NdeII GATC 2 cut(s) 615, 676
NgoMIV GCCGGC 1 cut(s) 581
NlaIII CATG 2 cut(s) 89, 269
NlaIV GGNNCC 2 cut(s) 76, 97
NmuCI GTSAC 1 cut(s) 598
PdiI GCCGGC 1 cut(s) 583
PfeI GAWTC 1 cut(s) 551
PfoI TCCNGGA 1 cut(s) 219
PkrI GCNGC 4 cut(s) 463, 466, 469, 709
PleI GAGTC 3 cut(s) 22, 316, 400
PpsI GAGTC 3 cut(s) 22, 316, 400
Psp124BI GAGCTC 1 cut(s) 247
PspN4I GGNNCC 2 cut(s) 76, 97
PsuI RGATCY 1 cut(s) 615
PvuII CAGCTG 2 cut(s) 557, 623
RsaI GTAC 2 cut(s) 82, 639
RsaNI GTAC 2 cut(s) 81, 638
SacI GAGCTC 1 cut(s) 247
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 4 cut(s) 462, 465, 468, 708
Sau3AI GATC 2 cut(s) 615, 676
ScaI AGTACT 1 cut(s) 639
SchI GAGTC 3 cut(s) 22, 316, 401
ScrFI CCNGG 2 cut(s) 221, 540
SduI GDGCHC 2 cut(s) 247, 273
SetI ASST 9 cut(s) 247, 376, 458, 493, 523, 559, 625, 668, 716
SfaNI GCATC 1 cut(s) 568
Sse9I AATT 8 cut(s) 46, 131, 154, 334, 383, 425, 545, 699
SsiI CCGC 2 cut(s) 118, 707
SspMI CTAG 1 cut(s) 715
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 2 cut(s) 219, 538
TaaI ACNGT 3 cut(s) 208, 604, 637
TaqI TCGA 2 cut(s) 124, 563
TasI AATT 8 cut(s) 46, 131, 154, 334, 383, 425, 545, 699
TatI WGTACW 1 cut(s) 637
TauI GCSGC 1 cut(s) 710
TfiI GAWTC 1 cut(s) 551
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 1 cut(s) 607
TseFI GTSAC 1 cut(s) 598
TseI GCWGC 3 cut(s) 461, 464, 467
Tsp45I GTSAC 1 cut(s) 598
TspDTI ATGAA 3 cut(s) 252, 268, 649
TspGWI ACGGA 1 cut(s) 581
TspRI CASTG 1 cut(s) 607
XapI RAATTY 4 cut(s) 131, 154, 383, 545
XspI CTAG 1 cut(s) 715
ZrmI AGTACT 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.