Rh3DG259600

Rhodanese-like domain-containing protein 9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0012197
Physical Location & Seq
Forward (+)
25709242 .. 25710816
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG259600.1

Sequence Viewer

Length: 747 bp
ATGAAGTTGCAGCTTATACAAACTACTACGATGGCAGGGATTGCGTTCGGCACTTGCTGTTCAACTATCTCTTCCACCAGCAATTTCCCCACTTCTTGGTTGAAACTTGAGACTCGTACTCATCGTGGAGGAACCATCTTAAAGAAGCCAAACAGTGTTAGAACTCTGGCAATTAGAGCAGAAGTGAACTTTGTAAATGCAGACGAAGCTAAAGAACTAATTGCAGAAGGATATACGGTTATTGATGTTCGGGACAAATCTCAATACGATCGAGCTCATATCAAATCATGTTATCATGTGCCTCTTTTCATTGAAAATCAAGACAATGACATCGGCACAATTGTAAAGAGGACTGTGCACAACAATTTCTCAGGTCTGTTCTTCGGGTTACCTTTCACTAAACCCAACCCTGAGTTTGTACAATCTGTTAAAGGCCAGTTTTCACCTCAGAGTAAACTGTTACTGGTGTGCCAGGAAGGATTGAGGTCTGCTGCTGCTGCTAGTAAGTTGGAGCAGGCTGGTTTCCAAGACATAGCATGTATAACATCAGGGCTGCAAAAAGTAAAACCAGGAGCATTTGATGCTGTTGGATCCACTGAGTTGCAAGATGCTGGCAAAGCAGGTTTGATCACGGTACAAGGAAAGATCTCAGCTGTACTTGGAACTGTACTAGTCTGTGCATATTTGTTCATCACCTTCTTCCCTGATCAAGCAGAGAAGCTACTTCAATTGGCTCCTACAGGCTAG

Protein Analysis

248

Amino Acids

26.88

Weight (kDa)

8.7

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 67 - 188 1.3e-10 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012197)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42220
fragaria_vesca FvH4_6g22700 FvH4_6g22700 FvH4_6g22700
malus_domestica MD12G1061100.v1.1 MD14G1060800.v1.1
prunus_persica Prupe.7G073600_v2.0.a1 Prupe.7G073600_v2.0.a1
pyrus_communis pycom12g05400
rosa_chinensis RchiOBHm_Chr3g0480311
rosa_laevigata RLG00000023493
rosa_multiflora Rmu_ssc0000353.1_g000001
rosa_roxburghii Rroxscaffold_6G00401570
rosa_rugosa Rorug03G0182400
rosa_samantha Rh3AG234300 Rh3BG266600 Rh3CG264100 Rh3DG259600
rosa_wichuraiana Rw3G021030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 611
AccB7I CCANNNNNTGG 1 cut(s) 96
AclWI GGATC 2 cut(s) 585, 598
AfaI GTAC 5 cut(s) 118, 420, 636, 657, 669
AfiI CCNNNNNNNGG 1 cut(s) 96
AgsI TTSAA 4 cut(s) 63, 103, 314, 728
AhlI ACTAGT 1 cut(s) 670
AjnI CCWGG 2 cut(s) 471, 568
AluBI AGCT 5 cut(s) 13, 209, 275, 653, 721
AluI AGCT 5 cut(s) 13, 209, 275, 653, 721
Alw21I GWGCWC 2 cut(s) 277, 360
Alw26I GTCTC 1 cut(s) 104
Alw44I GTGCAC 1 cut(s) 356
AlwI GGATC 2 cut(s) 585, 598
AoxI GGCC 1 cut(s) 433
ApaLI GTGCAC 1 cut(s) 356
ApeKI GCWGC 5 cut(s) 10, 491, 494, 497, 553
AsuHPI GGTGA 2 cut(s) 435, 685
BaeGI GKGCMC 1 cut(s) 360
BamHI GGATCC 1 cut(s) 590
BanII GRGCYC 1 cut(s) 277
Bbv12I GWGCWC 2 cut(s) 277, 360
BbvI GCAGC 5 cut(s) 22, 478, 481, 484, 540
BccI CCATC 2 cut(s) 25, 143
BciT130I CCWGG 2 cut(s) 473, 570
BclI TGATCA 2 cut(s) 627, 706
BcoDI GTCTC 1 cut(s) 104
BcuI ACTAGT 1 cut(s) 670
BfaI CTAG 3 cut(s) 501, 671, 745
BfmI CTRYAG 1 cut(s) 738
BfuAI ACCTGC 1 cut(s) 611
BglII AGATCT 1 cut(s) 645
BisI GCNGC 5 cut(s) 11, 492, 495, 498, 554
BlsI GCNGC 5 cut(s) 12, 493, 496, 499, 555
Bme1390I CCNGG 2 cut(s) 473, 570
BmiI GGNNCC 3 cut(s) 133, 592, 735
BmrFI CCNGG 2 cut(s) 473, 570
BmsI GCATC 2 cut(s) 571, 598
BpuEI CTTGAG 1 cut(s) 128
Bsc4I CCNNNNNNNGG 1 cut(s) 96
Bse1I ACTGG 2 cut(s) 436, 468
BseBI CCWGG 2 cut(s) 473, 570
BseLI CCNNNNNNNGG 1 cut(s) 96
BseMII CTCAG 5 cut(s) 384, 402, 461, 588, 663
BseNI ACTGG 2 cut(s) 436, 468
BseSI GKGCMC 1 cut(s) 360
BseXI GCAGC 5 cut(s) 22, 478, 481, 484, 540
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 1 cut(s) 435
BsiEI CGRYCG 1 cut(s) 271
BsiHKAI GWGCWC 2 cut(s) 277, 360
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 1 cut(s) 96
BsmAI GTCTC 1 cut(s) 104
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 1 cut(s) 435
Bsp1286I GDGCHC 2 cut(s) 277, 360
Bsp1407I TGTACA 1 cut(s) 418
Bsp143I GATC 5 cut(s) 268, 590, 627, 645, 706
BspANI GGCC 1 cut(s) 435
BspCNI CTCAG 5 cut(s) 383, 403, 460, 589, 662
BspLI GGNNCC 3 cut(s) 133, 592, 735
BspMI ACCTGC 1 cut(s) 611
BspPI GGATC 2 cut(s) 585, 598
BsrGI TGTACA 1 cut(s) 418
BsrI ACTGG 2 cut(s) 436, 468
BssMI GATC 5 cut(s) 268, 590, 627, 645, 706
Bst2UI CCWGG 2 cut(s) 473, 570
Bst4CI ACNGT 6 cut(s) 155, 238, 355, 459, 634, 667
Bst6I CTCTTC 1 cut(s) 76
BstAPI GCANNNNNTGC 2 cut(s) 41, 581
BstAUI TGTACA 1 cut(s) 418
BstC8I GCNNGC 2 cut(s) 516, 613
BstDEI CTNAG 5 cut(s) 370, 411, 447, 597, 649
BstEII GGTNACC 1 cut(s) 387
BstKTI GATC 5 cut(s) 271, 593, 630, 648, 709
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 5 cut(s) 268, 590, 627, 645, 706
BstMCI CGRYCG 1 cut(s) 271
BstMWI GCNNNNNNNGC 6 cut(s) 41, 176, 206, 497, 581, 617
BstNI CCWGG 2 cut(s) 473, 570
BstNSI RCATGY 1 cut(s) 540
BstPI GGTNACC 1 cut(s) 387
BstSCI CCNGG 2 cut(s) 471, 568
BstSFI CTRYAG 1 cut(s) 738
BstSLI GKGCMC 1 cut(s) 360
BstV1I GCAGC 5 cut(s) 22, 478, 481, 484, 540
BstX2I RGATCY 2 cut(s) 590, 645
BstYI RGATCY 2 cut(s) 590, 645
BsuRI GGCC 1 cut(s) 435
BtsIMutI CAGTG 2 cut(s) 160, 594
BveI ACCTGC 1 cut(s) 611
Cac8I GCNNGC 2 cut(s) 516, 613
Csp6I GTAC 5 cut(s) 117, 419, 635, 656, 668
CviAII CATG 3 cut(s) 288, 296, 537
CviQI GTAC 5 cut(s) 117, 419, 635, 656, 668
DdeI CTNAG 5 cut(s) 370, 411, 447, 597, 649
DpnI GATC 5 cut(s) 270, 592, 629, 647, 708
DpnII GATC 5 cut(s) 268, 590, 627, 645, 706
Eam1104I CTCTTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 76
Ecl136II GAGCTC 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 277
Eco53kI GAGCTC 1 cut(s) 275
Eco91I GGTNACC 1 cut(s) 387
EcoICRI GAGCTC 1 cut(s) 275
EcoO65I GGTNACC 1 cut(s) 387
EcoRII CCWGG 2 cut(s) 471, 568
EcoT38I GRGCYC 1 cut(s) 277
FaeI CATG 3 cut(s) 291, 299, 540
FaiI YATR 9 cut(s) 17, 234, 279, 289, 297, 533, 538, 542, 682
FaqI GGGAC 1 cut(s) 266
FatI CATG 3 cut(s) 287, 295, 536
FbaI TGATCA 2 cut(s) 627, 706
Fnu4HI GCNGC 5 cut(s) 11, 492, 495, 498, 554
FriOI GRGCYC 1 cut(s) 277
Fsp4HI GCNGC 5 cut(s) 11, 492, 495, 498, 554
FspBI CTAG 3 cut(s) 501, 671, 745
GluI GCNGC 5 cut(s) 11, 492, 495, 498, 554
HaeIII GGCC 1 cut(s) 435
Hin1II CATG 3 cut(s) 291, 299, 540
HinfI GANTC 1 cut(s) 112
HphI GGTGA 2 cut(s) 435, 685
Hpy166II GTNNAC 3 cut(s) 187, 358, 455
Hpy188I TCNGA 1 cut(s) 450
Hpy188III TCNNGA 2 cut(s) 251, 320
Hpy8I GTNNAC 3 cut(s) 187, 358, 455
HpyAV CCTTC 3 cut(s) 221, 470, 706
HpyCH4III ACNGT 6 cut(s) 155, 238, 355, 459, 634, 667
HpyCH4V TGCA 7 cut(s) 10, 200, 224, 358, 556, 604, 680
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 176, 206, 497, 581, 617
HpyF3I CTNAG 5 cut(s) 370, 411, 447, 597, 649
Hsp92II CATG 3 cut(s) 291, 299, 540
Ksp22I TGATCA 2 cut(s) 627, 706
Kzo9I GATC 5 cut(s) 268, 590, 627, 645, 706
LmnI GCTCC 3 cut(s) 511, 572, 739
Lsp1109I GCAGC 5 cut(s) 22, 478, 481, 484, 540
LweI GCATC 2 cut(s) 571, 598
MaeI CTAG 3 cut(s) 501, 671, 745
MaeIII GTNAC 2 cut(s) 387, 459
MalI GATC 5 cut(s) 270, 592, 629, 647, 708
MboI GATC 5 cut(s) 268, 590, 627, 645, 706
MboII GAAGA 3 cut(s) 63, 373, 691
MfeI CAATTG 2 cut(s) 339, 728
MflI RGATCY 2 cut(s) 590, 645
MhlI GDGCHC 2 cut(s) 277, 360
MluCI AATT 6 cut(s) 82, 171, 219, 339, 364, 728
MlyI GAGTC 1 cut(s) 106
MmeI TCCRAC 2 cut(s) 489, 568
MnlI CCTC 5 cut(s) 122, 312, 342, 456, 477
MseI TTAA 2 cut(s) 140, 429
MspA1I CMGCKG 1 cut(s) 653
MspR9I CCNGG 2 cut(s) 473, 570
MunI CAATTG 2 cut(s) 339, 728
MvaI CCWGG 2 cut(s) 473, 570
MwoI GCNNNNNNNGC 6 cut(s) 41, 176, 206, 497, 581, 617
NdeII GATC 5 cut(s) 268, 590, 627, 645, 706
NlaIII CATG 3 cut(s) 291, 299, 540
NlaIV GGNNCC 3 cut(s) 133, 592, 735
NspI RCATGY 1 cut(s) 540
PcsI WCGNNNNNNNCGW 1 cut(s) 121
PflMI CCANNNNNTGG 1 cut(s) 96
PkrI GCNGC 5 cut(s) 12, 493, 496, 499, 555
Ple19I CGATCG 1 cut(s) 271
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
Psp124BI GAGCTC 1 cut(s) 277
Psp6I CCWGG 2 cut(s) 471, 568
PspEI GGTNACC 1 cut(s) 387
PspGI CCWGG 2 cut(s) 471, 568
PspN4I GGNNCC 3 cut(s) 133, 592, 735
PsuI RGATCY 2 cut(s) 590, 645
PvuI CGATCG 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 653
RsaI GTAC 5 cut(s) 118, 420, 636, 657, 669
RsaNI GTAC 5 cut(s) 117, 419, 635, 656, 668
SacI GAGCTC 1 cut(s) 277
SaqAI TTAA 2 cut(s) 140, 429
SatI GCNGC 5 cut(s) 11, 492, 495, 498, 554
Sau3AI GATC 5 cut(s) 268, 590, 627, 645, 706
SchI GAGTC 1 cut(s) 106
ScrFI CCNGG 2 cut(s) 473, 570
SduI GDGCHC 2 cut(s) 277, 360
SfaNI GCATC 2 cut(s) 571, 598
SfcI CTRYAG 1 cut(s) 738
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
SpeI ACTAGT 1 cut(s) 670
Sse9I AATT 6 cut(s) 82, 171, 219, 339, 364, 728
SspMI CTAG 3 cut(s) 501, 671, 745
SstI GAGCTC 1 cut(s) 277
StyD4I CCNGG 2 cut(s) 471, 568
TaaI ACNGT 6 cut(s) 155, 238, 355, 459, 634, 667
TaqI TCGA 1 cut(s) 271
TasI AATT 6 cut(s) 82, 171, 219, 339, 364, 728
TatI WGTACW 3 cut(s) 418, 655, 667
Tru1I TTAA 2 cut(s) 140, 429
Tru9I TTAA 2 cut(s) 140, 429
TscAI CASTG 2 cut(s) 160, 601
TseI GCWGC 5 cut(s) 10, 491, 494, 497, 553
TspDTI ATGAA 3 cut(s) 17, 298, 679
TspRI CASTG 2 cut(s) 160, 601
Van91I CCANNNNNTGG 1 cut(s) 96
VneI GTGCAC 1 cut(s) 356
XceI RCATGY 1 cut(s) 540
XspI CTAG 3 cut(s) 501, 671, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.