AT2G43290

MSS3 MSS3 (multicopy suppressors of snf4 deficiency in yeast 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013958
Physical Location & Seq
Reverse (-)
17991052 .. 17992047
996 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G43290.1

Sequence Viewer

Length: 648 bp
ATGGTGAGAATATTCCTTCTCTACAATATACTAAATTCGTTTCTTCTCTCTTTAGTACCAAAGAAGCTACGAACTCTTTTCCCTCTTTCTTGGTTCGACAAAACTCTCCACAAGAACTCACCACCGTCTCCGTCAACGATGTTACCTTCTCCATCATCTTCTTCAGCGCCGACGAAAAGAATAGATCCGTCCGAGCTCAAACGCGTTTTCCAGATGTTCGACAAGAACGGTGACGGTCGAATCACAAAGGAAGAGCTCAACGACTCGCTTGAGAATCTTGGAATCTACATACCAGACAAAGATCTGACTCAAATGATCCACAAGATCGATGCTAACGGTGATGGATGCGTCGACATAGACGAGTTTGAGTCGCTGTACAGCTCGATTGTGGATGAGCATCACAACGATGGCGAAACAGAGGAAGAGGATATGAAAGATGCGTTTAACGTGTTTGACCAAGACGGAGATGGGTTTATCACTGTGGAGGAGTTGAAATCTGTGATGGCTTCCTTGGGACTCAAGCAAGGGAAGACCCTAGATGGTTGTAAGAAGATGATTATGCAAGTTGATGCAGATGGTGATGGTAGAGTCAATTACAAAGAGTTTCTTCAGATGATGAAAGGTGGTGGCTTTAGCAGCAGTAATTGA

Protein Analysis

215

Amino Acids

24.11

Weight (kDa)

4.76

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 64 - 127 1.1e-15 EF-hand domain pair
EF_EFCAB10_C PF24548 64 - 110 9.6e-06 EFCAB10, C-terminal EF-hand
EF-hand_1 PF00036 65 - 88 1.7e-07 EF hand domain
EF-hand_6 PF13405 65 - 94 8.9e-08 EF-hand domain
EF-hand_5 PF13202 66 - 87 8.9e-08 EF hand
AIF-1 PF21008 134 - 208 6e-07 Allograft inflammatory factor 1
EF-hand_7 PF13499 141 - 207 3e-15 EF-hand domain pair
EF-hand_6 PF13405 143 - 172 4.8e-09 EF-hand domain
EF-hand_1 PF00036 144 - 171 9.9e-09 EF hand domain
EF-hand_5 PF13202 144 - 168 2.2e-08 EF hand
EF-hand_8 PF13833 159 - 207 9.2e-06 EF-hand domain pair
EF-hand_1 PF00036 182 - 207 2.1e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43290
fragaria_vesca FvH4_4g10830
malus_domestica MD04G1041400.v1.1 MD13G1272800.v1.1
prunus_persica Prupe.1G194800_v2.0.a1
pyrus_communis pycom04g03640
rosa_chinensis RchiOBHm_Chr4g0406651
rosa_laevigata RLG00000008762
rosa_multiflora Rmu_co8120260.1_g000001 Rmu_sc0002774.1_g000003 Rmu_sc0019587.1_g000005
rosa_roxburghii Rroxscaffold_5G00351490
rosa_rugosa Rorug04G0072700
rosa_samantha Rh4AG140000 Rh4BG136900 Rh4DG134100
rosa_wichuraiana Rw4G011400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 351
AccII CGCG 1 cut(s) 204
AclWI GGATC 2 cut(s) 179, 310
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 2 cut(s) 147, 593
AfaI GTAC 2 cut(s) 57, 377
AflIII ACRYGT 2 cut(s) 202, 447
AgsI TTSAA 1 cut(s) 493
AluBI AGCT 4 cut(s) 67, 196, 256, 381
AluI AGCT 4 cut(s) 67, 196, 256, 381
Alw21I GWGCWC 2 cut(s) 198, 258
Alw26I GTCTC 1 cut(s) 132
AlwI GGATC 2 cut(s) 179, 310
ApeKI GCWGC 1 cut(s) 636
ApoI RAATTY 1 cut(s) 34
AspLEI GCGC 1 cut(s) 169
AsuHPI GGTGA 5 cut(s) 16, 111, 242, 350, 590
BanII GRGCYC 2 cut(s) 198, 258
BbsI GAAGAC 1 cut(s) 536
Bbv12I GWGCWC 2 cut(s) 198, 258
BccI CCATC 8 cut(s) 160, 335, 401, 461, 496, 533, 569, 575
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 1 cut(s) 536
BfoI RGCGCY 1 cut(s) 170
BglII AGATCT 1 cut(s) 301
BisI GCNGC 1 cut(s) 637
BlsI GCNGC 1 cut(s) 638
BmsI GCATC 5 cut(s) 319, 335, 406, 427, 559
BpiI GAAGAC 1 cut(s) 536
BpuEI CTTGAG 2 cut(s) 290, 503
Bsa29I ATCGAT 1 cut(s) 327
BsaBI GATNNNNATC 1 cut(s) 396
BsaJI CCNNGG 1 cut(s) 510
Bse8I GATNNNNATC 1 cut(s) 396
BseCI ATCGAT 1 cut(s) 327
BseDI CCNNGG 1 cut(s) 510
BseGI GGATG 2 cut(s) 350, 397
BseJI GATNNNNATC 1 cut(s) 396
BseRI GAGGAG 1 cut(s) 500
Bsh1236I CGCG 1 cut(s) 204
Bsh1285I CGRYCG 1 cut(s) 238
BshVI ATCGAT 1 cut(s) 327
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 2 cut(s) 198, 258
BslFI GGGAC 1 cut(s) 528
BsmAI GTCTC 1 cut(s) 132
BsmBI CGTCTC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 528
Bsp1286I GDGCHC 2 cut(s) 198, 258
Bsp1407I TGTACA 1 cut(s) 375
Bsp143I GATC 4 cut(s) 184, 301, 315, 324
BspDI ATCGAT 1 cut(s) 327
BspFNI CGCG 1 cut(s) 204
BspPI GGATC 2 cut(s) 179, 310
BspQI GCTCTTC 1 cut(s) 246
BsrGI TGTACA 1 cut(s) 375
BssECI CCNNGG 1 cut(s) 510
BssMI GATC 4 cut(s) 184, 301, 315, 324
BssT1I CCWWGG 1 cut(s) 510
Bst4CI ACNGT 5 cut(s) 126, 230, 236, 338, 481
Bst6I CTCTTC 2 cut(s) 246, 417
BstAUI TGTACA 1 cut(s) 375
BstF5I GGATG 2 cut(s) 350, 397
BstFNI CGCG 1 cut(s) 204
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 4 cut(s) 187, 304, 318, 327
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 4 cut(s) 184, 301, 315, 324
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 1 cut(s) 636
BstUI CGCG 1 cut(s) 204
BstV2I GAAGAC 1 cut(s) 536
BstX2I RGATCY 2 cut(s) 184, 301
BstYI RGATCY 2 cut(s) 184, 301
Bsu15I ATCGAT 1 cut(s) 327
BsuTUI ATCGAT 1 cut(s) 327
BtsCI GGATG 2 cut(s) 350, 397
BtsIMutI CAGTG 1 cut(s) 477
CfoI GCGC 1 cut(s) 169
ClaI ATCGAT 1 cut(s) 327
CseI GACGC 1 cut(s) 337
Csp6I GTAC 2 cut(s) 56, 376
CviJI RGCY 6 cut(s) 67, 196, 256, 381, 506, 630
CviKI_1 RGCY 6 cut(s) 67, 196, 256, 381, 506, 630
CviQI GTAC 2 cut(s) 56, 376
DpnI GATC 4 cut(s) 186, 303, 317, 326
DpnII GATC 4 cut(s) 184, 301, 315, 324
Eam1104I CTCTTC 2 cut(s) 246, 417
EarI CTCTTC 2 cut(s) 246, 417
Ecl136II GAGCTC 2 cut(s) 196, 256
Eco130I CCWWGG 1 cut(s) 510
Eco24I GRGCYC 2 cut(s) 198, 258
Eco53kI GAGCTC 2 cut(s) 196, 256
Eco57I CTGAAG 2 cut(s) 147, 593
EcoICRI GAGCTC 2 cut(s) 196, 256
EcoT14I CCWWGG 1 cut(s) 510
EcoT38I GRGCYC 2 cut(s) 198, 258
ErhI CCWWGG 1 cut(s) 510
Esp3I CGTCTC 1 cut(s) 132
FaiI YATR 5 cut(s) 29, 290, 356, 431, 560
FalI AAGNNNNNCTT 2 cut(s) 591, 623
FaqI GGGAC 1 cut(s) 528
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 1 cut(s) 637
FokI GGATG 2 cut(s) 357, 404
FriOI GRGCYC 2 cut(s) 198, 258
Fsp4HI GCNGC 1 cut(s) 637
FspBI CTAG 1 cut(s) 536
GlaI GCGC 1 cut(s) 168
GluI GCNGC 1 cut(s) 637
HaeII RGCGCY 1 cut(s) 170
HgaI GACGC 1 cut(s) 337
HhaI GCGC 1 cut(s) 169
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HincII GTYRAC 2 cut(s) 135, 352
HindII GTYRAC 2 cut(s) 135, 352
HinfI GANTC 8 cut(s) 240, 263, 274, 282, 307, 368, 516, 588
HphI GGTGA 5 cut(s) 16, 111, 242, 350, 590
Hpy166II GTNNAC 2 cut(s) 135, 352
Hpy188I TCNGA 3 cut(s) 193, 306, 612
Hpy188III TCNNGA 1 cut(s) 211
Hpy8I GTNNAC 2 cut(s) 135, 352
Hpy99I CGWCG 2 cut(s) 175, 353
HpyAV CCTTC 2 cut(s) 26, 156
HpyCH4III ACNGT 5 cut(s) 126, 230, 236, 338, 481
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 2 cut(s) 562, 572
HpyF10VI GCNNNNNNNGC 1 cut(s) 636
HpySE526I ACGT 1 cut(s) 447
HspAI GCGC 1 cut(s) 167
Kzo9I GATC 4 cut(s) 184, 301, 315, 324
LguI GCTCTTC 1 cut(s) 246
LpnPI CCDG 2 cut(s) 224, 306
LweI GCATC 5 cut(s) 319, 335, 406, 427, 559
MaeI CTAG 1 cut(s) 536
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 2 cut(s) 141, 230
MalI GATC 4 cut(s) 186, 303, 317, 326
MboI GATC 4 cut(s) 184, 301, 315, 324
MboII GAAGA 8 cut(s) 35, 150, 153, 263, 434, 541, 562, 599
MflI RGATCY 2 cut(s) 184, 301
MhlI GDGCHC 2 cut(s) 198, 258
MluCI AATT 3 cut(s) 34, 592, 643
MluI ACGCGT 1 cut(s) 202
MlyI GAGTC 5 cut(s) 257, 301, 377, 510, 597
MnlI CCTC 4 cut(s) 93, 412, 418, 478
MseI TTAA 1 cut(s) 444
MslI CAYNNNNRTG 1 cut(s) 405
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 1 cut(s) 636
NdeII GATC 4 cut(s) 184, 301, 315, 324
NmuCI GTSAC 1 cut(s) 230
PciSI GCTCTTC 1 cut(s) 246
PcsI WCGNNNNNNNCGW 1 cut(s) 357
PfeI GAWTC 3 cut(s) 240, 274, 282
PkrI GCNGC 1 cut(s) 638
PleI GAGTC 5 cut(s) 257, 301, 376, 510, 596
PpsI GAGTC 5 cut(s) 257, 301, 376, 510, 596
Psp124BI GAGCTC 2 cut(s) 198, 258
PsuI RGATCY 2 cut(s) 184, 301
RsaI GTAC 2 cut(s) 57, 377
RsaNI GTAC 2 cut(s) 56, 376
RseI CAYNNNNRTG 1 cut(s) 405
SacI GAGCTC 2 cut(s) 198, 258
SalI GTCGAC 1 cut(s) 350
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 1 cut(s) 444
SatI GCNGC 1 cut(s) 637
Sau3AI GATC 4 cut(s) 184, 301, 315, 324
SchI GAGTC 5 cut(s) 257, 301, 377, 510, 597
SduI GDGCHC 2 cut(s) 198, 258
SetI ASST 7 cut(s) 69, 148, 198, 258, 383, 450, 625
SfaNI GCATC 5 cut(s) 319, 335, 406, 427, 559
SmiMI CAYNNNNRTG 1 cut(s) 405
SmlI CTYRAG 2 cut(s) 269, 518
SmoI CTYRAG 2 cut(s) 269, 518
Sse9I AATT 3 cut(s) 34, 592, 643
SspI AATATT 1 cut(s) 12
SspMI CTAG 1 cut(s) 536
SstI GAGCTC 2 cut(s) 198, 258
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 5 cut(s) 126, 230, 236, 338, 481
TaiI ACGT 1 cut(s) 450
TaqI TCGA 6 cut(s) 96, 219, 238, 327, 351, 383
TasI AATT 3 cut(s) 34, 592, 643
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 3 cut(s) 240, 274, 282
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TscAI CASTG 1 cut(s) 484
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 1 cut(s) 636
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 2 cut(s) 446, 632
TspGWI ACGGA 3 cut(s) 120, 177, 477
TspRI CASTG 1 cut(s) 484
XapI RAATTY 1 cut(s) 34
XcmI CCANNNNNNNNNTGG 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 351
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.