Rh4BG136900

Calmodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0013958
Physical Location & Seq
Reverse (-)
24268742 .. 24269422
681 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG136900.1

Sequence Viewer

Length: 681 bp
ATGCCAACCATTTTGGTCAGGATTTTCCTTATTTACAATCTTCTTAATGCATTCCTCCTCTCTTTGGTGCCCAAAAAACTGAGACCTCTTCTCCCCTCGTGTTGGTTTCCTGACATAAACCAAAACGAAGCCAAAGCCAAAACCAGAACCAAAACAAGAACCAGTGTTGCTGACAAGAACAAGGGTCAGGGTCAGGGTCAGGATCAAGATCAGCTGAGAATCACAAGAATGGAGCCGACTGAGCTGAAGCGCGTGTTCCAAATGTTCGACCAGAACGGCGACGGGCGCATCAGCAGAAAGGAGCTGAGCGATTCGCTGGAGAACTTGGGCATCTTCATCCCTGACAAGGAGCTGTTCAACATGATCCAGAAGATCGACGTGGATGGAGACGGGTGCGTCGACATTGACGAGTTCGGAGAGCTGTACCAATCCATAATGGACGAGCGGAACGACGAGGAGGACATGAAGGAGGCATTCAACGTTTTCGATCAGAACGGTGATGGCTTCATCGCCGTCGACGAGTTGAGGTCCGTCTTGTCCTCCCTCGGCCTCAAGCAAGGCAAGTCCATAGAGGACTGCAAGAGGATGATTATGAAGGTCGACGTCGATGGAGATGGCAGGGTCAATTACAAGGAGTTCAAGCAGATGATGAAGGGAGGAGGCTTTAGCGCTTTAACATAG

Protein Analysis

226

Amino Acids

25.75

Weight (kDa)

4.97

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 80 - 143 2.3e-14 EF-hand domain pair
EF-hand_1 PF00036 81 - 106 9.9e-07 EF hand domain
EF-hand_6 PF13405 81 - 110 2.5e-07 EF-hand domain
EF-hand_5 PF13202 82 - 103 9.7e-07 EF hand
EF-hand_1 PF00036 117 - 143 6.1e-06 EF hand domain
EF-hand_7 PF13499 152 - 218 6e-16 EF-hand domain pair
EF-hand_6 PF13405 154 - 183 3.5e-08 EF-hand domain
EF-hand_1 PF00036 155 - 182 8.3e-07 EF hand domain
EF-hand_1 PF00036 192 - 218 3.1e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43290
fragaria_vesca FvH4_4g10830
malus_domestica MD04G1041400.v1.1 MD13G1272800.v1.1
prunus_persica Prupe.1G194800_v2.0.a1
pyrus_communis pycom04g03640
rosa_chinensis RchiOBHm_Chr4g0406651
rosa_laevigata RLG00000008762
rosa_multiflora Rmu_co8120260.1_g000001 Rmu_sc0002774.1_g000003 Rmu_sc0019587.1_g000005
rosa_roxburghii Rroxscaffold_5G00351490
rosa_rugosa Rorug04G0072700
rosa_samantha Rh4AG140000 Rh4BG136900 Rh4DG134100
rosa_wichuraiana Rw4G011400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
AatII GACGTC 1 cut(s) 606
AccB1I GGYRCC 1 cut(s) 67
AccBSI CCGCTC 1 cut(s) 445
AccI GTMKAC 3 cut(s) 399, 516, 600
AccII CGCG 1 cut(s) 252
AciI CCGC 1 cut(s) 445
AclI AACGTT 1 cut(s) 480
AclWI GGATC 2 cut(s) 210, 358
AcuI CTGAAG 1 cut(s) 266
AcyI GRCGYC 1 cut(s) 603
AfaI GTAC 1 cut(s) 425
AfeI AGCGCT 1 cut(s) 670
AfiI CCNNNNNNNGG 3 cut(s) 64, 102, 346
AgsI TTSAA 3 cut(s) 358, 478, 640
AjiI CACGTC 1 cut(s) 379
AluBI AGCT 5 cut(s) 214, 244, 304, 352, 421
AluI AGCT 5 cut(s) 214, 244, 304, 352, 421
Alw26I GTCTC 2 cut(s) 76, 381
AlwI GGATC 2 cut(s) 210, 358
Aor51HI AGCGCT 1 cut(s) 670
AoxI GGCC 1 cut(s) 547
AspLEI GCGC 3 cut(s) 252, 288, 671
AspS9I GGNCC 1 cut(s) 528
AsuHPI GGTGA 1 cut(s) 509
AvaII GGWCC 1 cut(s) 528
BaeGI GKGCMC 1 cut(s) 72
BanI GGYRCC 1 cut(s) 67
BauI CACGAG 1 cut(s) 97
BccI CCATC 4 cut(s) 377, 494, 602, 608
BceAI ACGGC 2 cut(s) 292, 497
BcoDI GTCTC 2 cut(s) 76, 381
BfoI RGCGCY 1 cut(s) 672
BlpI GCTNAGC 1 cut(s) 305
Bme18I GGWCC 1 cut(s) 528
BmgBI CACGTC 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 528
BmiI GGNNCC 2 cut(s) 69, 234
BmsI GCATC 2 cut(s) 297, 339
BpmI CTGGAG 1 cut(s) 338
Bpu1102I GCTNAGC 1 cut(s) 305
BpuEI CTTGAG 1 cut(s) 536
BsaBI GATNNNNATC 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 603
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 1 cut(s) 544
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 102, 346
Bse1I ACTGG 1 cut(s) 162
Bse8I GATNNNNATC 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 544
BseGI GGATG 3 cut(s) 336, 388, 591
BseJI GATNNNNATC 1 cut(s) 207
BseLI CCNNNNNNNGG 3 cut(s) 64, 102, 346
BseMII CTCAG 4 cut(s) 71, 206, 231, 296
BseNI ACTGG 1 cut(s) 162
BseRI GAGGAG 3 cut(s) 47, 470, 672
BseSI GKGCMC 1 cut(s) 72
Bsh1236I CGCG 1 cut(s) 252
BshFI GGCC 1 cut(s) 549
BshNI GGYRCC 1 cut(s) 67
BslI CCNNNNNNNGG 3 cut(s) 64, 102, 346
BsmAI GTCTC 2 cut(s) 76, 381
BsmBI CGTCTC 1 cut(s) 381
BsmI GAATGC 2 cut(s) 50, 473
BsnI GGCC 1 cut(s) 549
Bso31I GGTCTC 1 cut(s) 76
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 5 cut(s) 202, 208, 363, 372, 487
Bsp1720I GCTNAGC 1 cut(s) 305
BspACI CCGC 1 cut(s) 445
BspANI GGCC 1 cut(s) 549
BspCNI CTCAG 4 cut(s) 72, 207, 232, 297
BspFNI CGCG 1 cut(s) 252
BspLI GGNNCC 2 cut(s) 69, 234
BspPI GGATC 2 cut(s) 210, 358
BspT107I GGYRCC 1 cut(s) 67
BspTNI GGTCTC 1 cut(s) 76
BsrBI CCGCTC 1 cut(s) 445
BsrI ACTGG 1 cut(s) 162
BssECI CCNNGG 1 cut(s) 544
BssMI GATC 5 cut(s) 202, 208, 363, 372, 487
BssNI GRCGYC 1 cut(s) 603
BssSI CACGAG 1 cut(s) 97
Bst2BI CACGAG 1 cut(s) 97
Bst4CI ACNGT 1 cut(s) 497
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 603
BstDEI CTNAG 4 cut(s) 80, 215, 240, 305
BstF5I GGATG 3 cut(s) 336, 388, 591
BstFNI CGCG 1 cut(s) 252
BstH2I RGCGCY 1 cut(s) 672
BstHHI GCGC 3 cut(s) 252, 288, 671
BstKTI GATC 5 cut(s) 205, 211, 366, 375, 490
BstMAI GTCTC 2 cut(s) 76, 381
BstMBI GATC 5 cut(s) 202, 208, 363, 372, 487
BstMWI GCNNNNNNNGC 2 cut(s) 241, 285
BstSLI GKGCMC 1 cut(s) 72
BstUI CGCG 1 cut(s) 252
BsuRI GGCC 1 cut(s) 549
BtgZI GCGATG 1 cut(s) 493
BtrI CACGTC 1 cut(s) 379
BtsCI GGATG 3 cut(s) 336, 388, 591
BtsIMutI CAGTG 1 cut(s) 169
CfoI GCGC 3 cut(s) 252, 288, 671
Cfr13I GGNCC 1 cut(s) 528
CseI GACGC 1 cut(s) 385
Csp6I GTAC 1 cut(s) 424
CviAII CATG 2 cut(s) 361, 463
CviQI GTAC 1 cut(s) 424
DdeI CTNAG 4 cut(s) 80, 215, 240, 305
DpnI GATC 5 cut(s) 204, 210, 365, 374, 489
DpnII GATC 5 cut(s) 202, 208, 363, 372, 487
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
Eco31I GGTCTC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 528
Eco47III AGCGCT 1 cut(s) 670
Eco57I CTGAAG 1 cut(s) 266
EcoT22I ATGCAT 1 cut(s) 52
Esp3I CGTCTC 1 cut(s) 381
FaeI CATG 2 cut(s) 364, 466
FaiI YATR 7 cut(s) 116, 362, 434, 464, 569, 593, 679
FatI CATG 2 cut(s) 360, 462
FblI GTMKAC 3 cut(s) 399, 516, 600
FokI GGATG 3 cut(s) 323, 395, 598
GlaI GCGC 3 cut(s) 251, 287, 670
GsuI CTGGAG 1 cut(s) 338
HaeII RGCGCY 1 cut(s) 672
HaeIII GGCC 1 cut(s) 549
HgaI GACGC 1 cut(s) 385
HhaI GCGC 3 cut(s) 252, 288, 671
Hin1I GRCGYC 1 cut(s) 603
Hin1II CATG 2 cut(s) 364, 466
Hin6I GCGC 3 cut(s) 250, 286, 669
HinP1I GCGC 3 cut(s) 250, 286, 669
HincII GTYRAC 3 cut(s) 400, 517, 601
HindII GTYRAC 3 cut(s) 400, 517, 601
HinfI GANTC 2 cut(s) 219, 311
HphI GGTGA 1 cut(s) 509
Hpy166II GTNNAC 3 cut(s) 400, 517, 601
Hpy188I TCNGA 2 cut(s) 416, 492
Hpy188III TCNNGA 5 cut(s) 19, 110, 200, 206, 367
Hpy8I GTNNAC 3 cut(s) 400, 517, 601
Hpy99I CGWCG 8 cut(s) 284, 380, 401, 455, 518, 521, 605, 608
HpyAV CCTTC 3 cut(s) 460, 589, 646
HpyCH4III ACNGT 1 cut(s) 497
HpyCH4IV ACGT 3 cut(s) 378, 480, 603
HpyCH4V TGCA 2 cut(s) 50, 579
HpyF10VI GCNNNNNNNGC 2 cut(s) 241, 285
HpyF3I CTNAG 4 cut(s) 80, 215, 240, 305
HpySE526I ACGT 3 cut(s) 378, 480, 603
Hsp92I GRCGYC 1 cut(s) 603
Hsp92II CATG 2 cut(s) 364, 466
HspAI GCGC 3 cut(s) 250, 286, 669
Kzo9I GATC 5 cut(s) 202, 208, 363, 372, 487
LmnI GCTCC 3 cut(s) 232, 301, 349
LweI GCATC 2 cut(s) 297, 339
MaeII ACGT 3 cut(s) 378, 480, 603
MalI GATC 5 cut(s) 204, 210, 365, 374, 489
MbiI CCGCTC 1 cut(s) 445
MboI GATC 5 cut(s) 202, 208, 363, 372, 487
MboII GAAGA 4 cut(s) 32, 80, 325, 382
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 1 cut(s) 625
Mph1103I ATGCAT 1 cut(s) 52
MseI TTAA 2 cut(s) 45, 674
MslI CAYNNNNRTG 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 214
Mva1269I GAATGC 2 cut(s) 50, 473
MvnI CGCG 1 cut(s) 252
MwoI GCNNNNNNNGC 2 cut(s) 241, 285
NdeII GATC 5 cut(s) 202, 208, 363, 372, 487
NlaIII CATG 2 cut(s) 364, 466
NlaIV GGNNCC 2 cut(s) 69, 234
NmeAIII GCCGAG 1 cut(s) 525
NsiI ATGCAT 1 cut(s) 52
PcsI WCGNNNNNNNCGW 4 cut(s) 396, 405, 447, 516
PctI GAATGC 2 cut(s) 50, 473
PfeI GAWTC 2 cut(s) 219, 311
Psp1406I AACGTT 1 cut(s) 480
PspN4I GGNNCC 2 cut(s) 69, 234
PspPI GGNCC 1 cut(s) 528
PvuII CAGCTG 1 cut(s) 214
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
RseI CAYNNNNRTG 1 cut(s) 227
SalI GTCGAC 3 cut(s) 398, 515, 599
SaqAI TTAA 2 cut(s) 45, 674
Sau3AI GATC 5 cut(s) 202, 208, 363, 372, 487
Sau96I GGNCC 1 cut(s) 528
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 2 cut(s) 297, 339
SgrDI CGTCGACG 1 cut(s) 515
SinI GGWCC 1 cut(s) 528
SmiMI CAYNNNNRTG 1 cut(s) 227
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
Sse9I AATT 1 cut(s) 625
SsiI CCGC 1 cut(s) 445
TaaI ACNGT 1 cut(s) 497
TaiI ACGT 3 cut(s) 381, 483, 606
TaqI TCGA 7 cut(s) 267, 375, 399, 486, 516, 600, 606
TasI AATT 1 cut(s) 625
TfiI GAWTC 2 cut(s) 219, 311
Tru1I TTAA 2 cut(s) 45, 674
Tru9I TTAA 2 cut(s) 45, 674
TscAI CASTG 1 cut(s) 169
TspDTI ATGAA 5 cut(s) 325, 479, 496, 608, 665
TspGWI ACGGA 1 cut(s) 520
TspRI CASTG 1 cut(s) 169
VpaK11BI GGWCC 1 cut(s) 528
XmiI GTMKAC 3 cut(s) 399, 516, 600
ZraI GACGTC 1 cut(s) 604
Zsp2I ATGCAT 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.