MD04G1041400.v1.1

Calmodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013958
Physical Location & Seq
Forward (+)
4909170 .. 4910255
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1041400.v1.1.491

Sequence Viewer

Length: 633 bp
ATGCCAACCATTTTCCCAAGGATTTTCCTTATTTACAATCTTCTTAATACATTCCTCCTCTCTTTGGTCCCCAAGCATCTCAGGCATCTCCTCCCCTCCTCCTGGTTCCCCCACCACACCACTCTTCTTGACACCAAAACACCGTCACCCCAACCCCCACCTCCGTCGTCGTTGTCTTTGCCCTTGCCCTTGCCTTCTGGCGGTGCTTGTCACGTCAGAATGGACCCCAATGAGCTCAAACGCGTGTTCCAGATGTTTGATCGCAATGGGGATGGGCGGATCACCAAGCAGGAGCTCAACGACTCCTTGGAGAACCTGGGAATCTACATCCCGGACAAGGAGCTCTTCAACATGATCGAAAAGATTGACGTGAACGGTGATGGGTGCGTGGACATTGACGAGTTCGGGGAGTTGTACCAGTCTATCATGGACGAGCGCGACGAGGAGGAGGACATGAAGGAGGCATTCAACGTGTTTGATCAAAACGGAGACGGGTTCATAACCGTCGATGAGTTGAGGTCGGTTTTGTCTTCCCTCGGGCTTAAGCAAGGGAGGACTATAGAGGACTGCAAGAGGATGATCATGAAGGTGGATGTGGACGGAGATGGGAGGAGGGGGAATAGGAGGGCATAG

Protein Analysis

211

Amino Acids

23.92

Weight (kDa)

4.97

Isoelectric Point (pI)

54.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 77 - 140 4.4e-14 EF-hand domain pair
EF-hand_1 PF00036 78 - 101 5.1e-07 EF hand domain
EF-hand_6 PF13405 78 - 107 1.7e-07 EF-hand domain
EF-hand_5 PF13202 79 - 100 2.8e-07 EF hand
EF-hand_7 PF13499 149 - 204 1.1e-10 EF-hand domain pair
EF-hand_6 PF13405 151 - 180 1.9e-09 EF-hand domain
EF-hand_1 PF00036 152 - 179 1.9e-08 EF hand domain
EF-hand_5 PF13202 153 - 176 1.3e-07 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43290
fragaria_vesca FvH4_4g10830
malus_domestica MD04G1041400.v1.1 MD13G1272800.v1.1
prunus_persica Prupe.1G194800_v2.0.a1
pyrus_communis pycom04g03640
rosa_chinensis RchiOBHm_Chr4g0406651
rosa_laevigata RLG00000008762
rosa_multiflora Rmu_co8120260.1_g000001 Rmu_sc0002774.1_g000003 Rmu_sc0019587.1_g000005
rosa_roxburghii Rroxscaffold_5G00351490
rosa_rugosa Rorug04G0072700
rosa_samantha Rh4AG140000 Rh4BG136900 Rh4DG134100
rosa_wichuraiana Rw4G011400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 243, 438
AciI CCGC 2 cut(s) 201, 277
AclWI GGATC 1 cut(s) 287
AfaI GTAC 1 cut(s) 416
AfiI CCNNNNNNNGG 4 cut(s) 64, 102, 200, 337
AflII CTTAAG 1 cut(s) 542
AflIII ACRYGT 2 cut(s) 241, 471
AgsI TTSAA 2 cut(s) 349, 469
AjiI CACGTC 2 cut(s) 214, 370
AjnI CCWGG 2 cut(s) 101, 315
AluBI AGCT 3 cut(s) 235, 295, 343
AluI AGCT 3 cut(s) 235, 295, 343
Alw21I GWGCWC 3 cut(s) 237, 297, 345
Alw26I GTCTC 1 cut(s) 483
AlwI GGATC 1 cut(s) 287
Ama87I CYCGRG 1 cut(s) 536
AspLEI GCGC 1 cut(s) 438
AspS9I GGNCC 2 cut(s) 67, 223
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 3 cut(s) 138, 274, 389
AvaI CYCGRG 1 cut(s) 536
AvaII GGWCC 2 cut(s) 67, 223
BanII GRGCYC 3 cut(s) 237, 297, 345
BbsI GAAGAC 1 cut(s) 522
Bbv12I GWGCWC 3 cut(s) 237, 297, 345
BccI CCATC 3 cut(s) 266, 374, 599
BciT130I CCWGG 2 cut(s) 103, 317
BclI TGATCA 2 cut(s) 478, 579
BcnI CCSGG 1 cut(s) 332
BcoDI GTCTC 1 cut(s) 483
BfmI CTRYAG 1 cut(s) 558
BfrI CTTAAG 1 cut(s) 542
Bme1390I CCNGG 3 cut(s) 103, 317, 332
Bme18I GGWCC 2 cut(s) 67, 223
BmeT110I CYCGRG 1 cut(s) 536
BmgBI CACGTC 2 cut(s) 214, 370
BmgT120I GGNCC 2 cut(s) 67, 223
BmiI GGNNCC 3 cut(s) 69, 107, 225
BmrFI CCNGG 3 cut(s) 103, 317, 332
BmsI GCATC 2 cut(s) 85, 94
BpiI GAAGAC 1 cut(s) 522
BpuMI CCSGG 1 cut(s) 332
BsaJI CCNNGG 4 cut(s) 17, 306, 316, 535
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 102, 200, 337
Bse1I ACTGG 1 cut(s) 418
Bse3DI GCAATG 1 cut(s) 271
BseBI CCWGG 2 cut(s) 103, 317
BseDI CCNNGG 4 cut(s) 17, 306, 316, 535
BseGI GGATG 4 cut(s) 277, 327, 582, 598
BseLI CCNNNNNNNGG 4 cut(s) 64, 102, 200, 337
BseMI GCAATG 1 cut(s) 271
BseMII CTCAG 1 cut(s) 94
BseNI ACTGG 1 cut(s) 418
BseRI GAGGAG 6 cut(s) 47, 80, 88, 458, 461, 625
Bsh1236I CGCG 2 cut(s) 243, 438
BsiHKAI GWGCWC 3 cut(s) 237, 297, 345
BsiHKCI CYCGRG 1 cut(s) 536
BsiSI CCGG 1 cut(s) 332
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 4 cut(s) 64, 102, 200, 337
BsmAI GTCTC 1 cut(s) 483
BsmBI CGTCTC 1 cut(s) 483
BsmFI GGGAC 1 cut(s) 53
BsmI GAATGC 1 cut(s) 464
BsoBI CYCGRG 1 cut(s) 536
Bsp1286I GDGCHC 3 cut(s) 237, 297, 345
Bsp143I GATC 5 cut(s) 259, 279, 354, 478, 579
BspACI CCGC 2 cut(s) 201, 277
BspCNI CTCAG 1 cut(s) 93
BspFNI CGCG 2 cut(s) 243, 438
BspHI TCATGA 1 cut(s) 582
BspLI GGNNCC 3 cut(s) 69, 107, 225
BspPI GGATC 1 cut(s) 287
BspQI GCTCTTC 1 cut(s) 350
BspTI CTTAAG 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 271
BsrI ACTGG 1 cut(s) 418
BssECI CCNNGG 4 cut(s) 17, 306, 316, 535
BssMI GATC 5 cut(s) 259, 279, 354, 478, 579
BssT1I CCWWGG 2 cut(s) 17, 306
Bst2UI CCWGG 2 cut(s) 103, 317
Bst4CI ACNGT 3 cut(s) 144, 377, 505
Bst6I CTCTTC 2 cut(s) 129, 350
BstAFI CTTAAG 1 cut(s) 542
BstDEI CTNAG 1 cut(s) 80
BstF5I GGATG 4 cut(s) 277, 327, 582, 598
BstFNI CGCG 2 cut(s) 243, 438
BstHHI GCGC 1 cut(s) 438
BstKTI GATC 5 cut(s) 262, 282, 357, 481, 582
BstMAI GTCTC 1 cut(s) 483
BstMBI GATC 5 cut(s) 259, 279, 354, 478, 579
BstMWI GCNNNNNNNGC 1 cut(s) 82
BstNI CCWGG 2 cut(s) 103, 317
BstSCI CCNGG 3 cut(s) 101, 315, 330
BstSFI CTRYAG 1 cut(s) 558
BstUI CGCG 2 cut(s) 243, 438
BstV2I GAAGAC 1 cut(s) 522
BtrI CACGTC 2 cut(s) 214, 370
BtsCI GGATG 4 cut(s) 277, 327, 582, 598
CciI TCATGA 1 cut(s) 582
CfoI GCGC 1 cut(s) 438
Cfr13I GGNCC 2 cut(s) 67, 223
Csp6I GTAC 1 cut(s) 415
CspCI CAANNNNNGTGG 2 cut(s) 109, 144
CviAII CATG 4 cut(s) 352, 427, 454, 583
CviJI RGCY 4 cut(s) 235, 295, 343, 541
CviKI_1 RGCY 4 cut(s) 235, 295, 343, 541
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 1 cut(s) 80
DpnI GATC 5 cut(s) 261, 281, 356, 480, 581
DpnII GATC 5 cut(s) 259, 279, 354, 478, 579
Eam1104I CTCTTC 2 cut(s) 129, 350
EarI CTCTTC 2 cut(s) 129, 350
EciI GGCGGA 1 cut(s) 292
Ecl136II GAGCTC 3 cut(s) 235, 295, 343
Eco130I CCWWGG 2 cut(s) 17, 306
Eco24I GRGCYC 3 cut(s) 237, 297, 345
Eco47I GGWCC 2 cut(s) 67, 223
Eco53kI GAGCTC 3 cut(s) 235, 295, 343
Eco88I CYCGRG 1 cut(s) 536
EcoICRI GAGCTC 3 cut(s) 235, 295, 343
EcoRII CCWGG 2 cut(s) 101, 315
EcoT14I CCWWGG 2 cut(s) 17, 306
EcoT38I GRGCYC 3 cut(s) 237, 297, 345
ErhI CCWWGG 2 cut(s) 17, 306
Esp3I CGTCTC 1 cut(s) 483
FaeI CATG 4 cut(s) 355, 430, 457, 586
FaiI YATR 7 cut(s) 353, 428, 455, 500, 560, 584, 631
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 1 cut(s) 53
FatI CATG 4 cut(s) 351, 426, 453, 582
FbaI TGATCA 2 cut(s) 478, 579
FokI GGATG 4 cut(s) 284, 314, 589, 605
FriOI GRGCYC 3 cut(s) 237, 297, 345
GlaI GCGC 1 cut(s) 437
HapII CCGG 1 cut(s) 332
HhaI GCGC 1 cut(s) 438
Hin1II CATG 4 cut(s) 355, 430, 457, 586
Hin6I GCGC 1 cut(s) 436
HinP1I GCGC 1 cut(s) 436
HinfI GANTC 2 cut(s) 302, 321
HpaII CCGG 1 cut(s) 332
HphI GGTGA 3 cut(s) 138, 274, 389
Hpy166II GTNNAC 3 cut(s) 373, 391, 598
Hpy188I TCNGA 1 cut(s) 218
Hpy188III TCNNGA 3 cut(s) 128, 250, 583
Hpy8I GTNNAC 3 cut(s) 373, 391, 598
Hpy99I CGWCG 4 cut(s) 169, 172, 443, 509
HpyAV CCTTC 3 cut(s) 204, 451, 580
HpyCH4III ACNGT 3 cut(s) 144, 377, 505
HpyCH4IV ACGT 3 cut(s) 213, 369, 471
HpyCH4V TGCA 1 cut(s) 570
HpyF10VI GCNNNNNNNGC 1 cut(s) 82
HpyF3I CTNAG 1 cut(s) 80
HpySE526I ACGT 3 cut(s) 213, 369, 471
Hsp92II CATG 4 cut(s) 355, 430, 457, 586
HspAI GCGC 1 cut(s) 436
Ksp22I TGATCA 2 cut(s) 478, 579
Kzo9I GATC 5 cut(s) 259, 279, 354, 478, 579
LguI GCTCTTC 1 cut(s) 350
LmnI GCTCC 2 cut(s) 292, 340
LweI GCATC 2 cut(s) 85, 94
MaeII ACGT 3 cut(s) 213, 369, 471
MaeIII GTNAC 2 cut(s) 144, 209
MalI GATC 5 cut(s) 261, 281, 356, 480, 581
MboI GATC 5 cut(s) 259, 279, 354, 478, 579
MboII GAAGA 4 cut(s) 32, 116, 337, 522
MhlI GDGCHC 3 cut(s) 237, 297, 345
MluI ACGCGT 1 cut(s) 241
MlyI GAGTC 1 cut(s) 296
MseI TTAA 2 cut(s) 45, 543
MslI CAYNNNNRTG 1 cut(s) 587
MspCI CTTAAG 1 cut(s) 542
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 3 cut(s) 103, 317, 332
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 2 cut(s) 103, 317
MvnI CGCG 2 cut(s) 243, 438
MwoI GCNNNNNNNGC 1 cut(s) 82
NciI CCSGG 1 cut(s) 332
NdeII GATC 5 cut(s) 259, 279, 354, 478, 579
NlaIII CATG 4 cut(s) 355, 430, 457, 586
NlaIV GGNNCC 3 cut(s) 69, 107, 225
NmuCI GTSAC 2 cut(s) 144, 209
PagI TCATGA 1 cut(s) 582
PciSI GCTCTTC 1 cut(s) 350
PcsI WCGNNNNNNNCGW 1 cut(s) 438
PctI GAATGC 1 cut(s) 464
PfeI GAWTC 1 cut(s) 321
PfoI TCCNGGA 1 cut(s) 330
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
Psp124BI GAGCTC 3 cut(s) 237, 297, 345
Psp6I CCWGG 2 cut(s) 101, 315
PspGI CCWGG 2 cut(s) 101, 315
PspN4I GGNNCC 3 cut(s) 69, 107, 225
PspPI GGNCC 2 cut(s) 67, 223
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
RseI CAYNNNNRTG 1 cut(s) 587
SacI GAGCTC 3 cut(s) 237, 297, 345
SapI GCTCTTC 1 cut(s) 350
SaqAI TTAA 2 cut(s) 45, 543
Sau3AI GATC 5 cut(s) 259, 279, 354, 478, 579
Sau96I GGNCC 2 cut(s) 67, 223
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 3 cut(s) 103, 317, 332
SduI GDGCHC 3 cut(s) 237, 297, 345
SfaNI GCATC 2 cut(s) 85, 94
SfcI CTRYAG 1 cut(s) 558
SinI GGWCC 2 cut(s) 67, 223
SmiMI CAYNNNNRTG 1 cut(s) 587
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
SsiI CCGC 2 cut(s) 201, 277
SstI GAGCTC 3 cut(s) 237, 297, 345
StyD4I CCNGG 3 cut(s) 101, 315, 330
StyI CCWWGG 2 cut(s) 17, 306
TaaI ACNGT 3 cut(s) 144, 377, 505
TaiI ACGT 3 cut(s) 216, 372, 474
TaqI TCGA 2 cut(s) 357, 507
TfiI GAWTC 1 cut(s) 321
Tru1I TTAA 2 cut(s) 45, 543
Tru9I TTAA 2 cut(s) 45, 543
TseFI GTSAC 2 cut(s) 144, 209
Tsp45I GTSAC 2 cut(s) 144, 209
TspDTI ATGAA 3 cut(s) 470, 487, 599
TspGWI ACGGA 3 cut(s) 153, 501, 615
Vha464I CTTAAG 1 cut(s) 542
VpaK11BI GGWCC 2 cut(s) 67, 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.