Rh4AG140000

Calmodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0013958
Physical Location & Seq
Reverse (-)
35579878 .. 35580552
675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG140000.1

Sequence Viewer

Length: 675 bp
ATGCCAACCATTTTGGTCAGGATTTTCCTTATTTACAATCTTCTTAATGCATTCCTCCTCTCTTTGGTACCCAAAAAACTGAGACCTCTTCTCCCCTCGTGTTGGTTTCCTGACATAAACCAAAACGAAGCCAAAGCCAAAACCAGAACCAAAACAAGAACCAGTGTTGCTGACAAGAACAAGGGTCAGGGTCAGGATCAAGATCAGCTGAGATTCACAAGAATGGAGCCGACTGAGCTGAAGCGCGTGTTCCAAATGTTCGACCAGAACGGCGACGGGCGCATCAGCAGAAAGGAGCTGGGCGATTCGCTGGAGAACTTGGGCATCTTCATCCCTGACAAGGAGCTGTTCAACATGATCCAGAAGATCGATGTCGACGGAGACGGGTGCGTCGACATTGACGAGTTCGGAGAGCTGTACCAATCCATAATGGACGAGCGGAACGAGGAGGAGGACATGAAGGAGGCATTCAACGTTTTCGATCAGAACGGTGATGGCTTCATCGCCGTCGACGAGTTGAGGTCCGTCTTGTCCTCCCTCGGCCTCAAGCAAGGCAAGTCCATAGAGGACTGCAAGAGGATGATTATGAATGTCGACGTCGATGGAGATGGCAGGGTCAATTACAAGGAGTTCAAGCAGATGATGAAGGGAGGAGGCTTTAGCGCTTTAACATAG

Protein Analysis

224

Amino Acids

25.57

Weight (kDa)

4.9

Isoelectric Point (pI)

36.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 78 - 141 2.6e-14 EF-hand domain pair
EF-hand_1 PF00036 79 - 104 8e-07 EF hand domain
EF-hand_6 PF13405 79 - 108 1.2e-07 EF-hand domain
EF-hand_5 PF13202 80 - 101 1e-06 EF hand
EF-hand_1 PF00036 115 - 141 6.1e-06 EF hand domain
AIF-1 PF21008 144 - 218 8.6e-06 Allograft inflammatory factor 1
EF-hand_7 PF13499 150 - 216 8.6e-16 EF-hand domain pair
EF-hand_6 PF13405 152 - 181 3.4e-08 EF-hand domain
EF-hand_1 PF00036 153 - 180 8.2e-07 EF hand domain
EF-hand_1 PF00036 190 - 216 1.1e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43290
fragaria_vesca FvH4_4g10830
malus_domestica MD04G1041400.v1.1 MD13G1272800.v1.1
prunus_persica Prupe.1G194800_v2.0.a1
pyrus_communis pycom04g03640
rosa_chinensis RchiOBHm_Chr4g0406651
rosa_laevigata RLG00000008762
rosa_multiflora Rmu_co8120260.1_g000001 Rmu_sc0002774.1_g000003 Rmu_sc0019587.1_g000005
rosa_roxburghii Rroxscaffold_5G00351490
rosa_rugosa Rorug04G0072700
rosa_samantha Rh4AG140000 Rh4BG136900 Rh4DG134100
rosa_wichuraiana Rw4G011400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 389
AatII GACGTC 1 cut(s) 600
Acc65I GGTACC 1 cut(s) 67
AccB1I GGYRCC 1 cut(s) 67
AccBSI CCGCTC 1 cut(s) 439
AccI GTMKAC 4 cut(s) 375, 393, 510, 594
AccII CGCG 1 cut(s) 246
AciI CCGC 1 cut(s) 439
AclI AACGTT 1 cut(s) 474
AclWI GGATC 2 cut(s) 204, 352
AcuI CTGAAG 1 cut(s) 260
AcyI GRCGYC 1 cut(s) 597
AfaI GTAC 2 cut(s) 69, 419
AfeI AGCGCT 1 cut(s) 664
AfiI CCNNNNNNNGG 3 cut(s) 64, 102, 340
AgsI TTSAA 3 cut(s) 352, 472, 634
AluBI AGCT 5 cut(s) 208, 238, 298, 346, 415
AluI AGCT 5 cut(s) 208, 238, 298, 346, 415
Alw26I GTCTC 2 cut(s) 76, 375
AlwI GGATC 2 cut(s) 204, 352
Aor51HI AGCGCT 1 cut(s) 664
AoxI GGCC 1 cut(s) 541
Asp718I GGTACC 1 cut(s) 67
AspLEI GCGC 3 cut(s) 246, 282, 665
AspS9I GGNCC 1 cut(s) 522
AsuHPI GGTGA 1 cut(s) 503
AvaII GGWCC 1 cut(s) 522
BanI GGYRCC 1 cut(s) 67
BauI CACGAG 1 cut(s) 97
BccI CCATC 3 cut(s) 488, 596, 602
BceAI ACGGC 2 cut(s) 286, 491
BcoDI GTCTC 2 cut(s) 76, 375
BfoI RGCGCY 1 cut(s) 666
Bme18I GGWCC 1 cut(s) 522
BmgT120I GGNCC 1 cut(s) 522
BmiI GGNNCC 2 cut(s) 69, 228
BmsI GCATC 2 cut(s) 291, 333
BpmI CTGGAG 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 530
Bsa29I ATCGAT 1 cut(s) 369
BsaBI GATNNNNATC 1 cut(s) 201
BsaHI GRCGYC 1 cut(s) 597
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 1 cut(s) 538
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 102, 340
Bse1I ACTGG 1 cut(s) 162
Bse8I GATNNNNATC 1 cut(s) 201
BseCI ATCGAT 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 538
BseGI GGATG 2 cut(s) 330, 585
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 3 cut(s) 64, 102, 340
BseMII CTCAG 3 cut(s) 71, 200, 225
BseNI ACTGG 1 cut(s) 162
BseRI GAGGAG 4 cut(s) 47, 461, 464, 666
BseYI CCCAGC 1 cut(s) 298
Bsh1236I CGCG 1 cut(s) 246
BshFI GGCC 1 cut(s) 543
BshNI GGYRCC 1 cut(s) 67
BshVI ATCGAT 1 cut(s) 369
BslI CCNNNNNNNGG 3 cut(s) 64, 102, 340
BsmAI GTCTC 2 cut(s) 76, 375
BsmBI CGTCTC 1 cut(s) 375
BsmI GAATGC 2 cut(s) 50, 467
BsnI GGCC 1 cut(s) 543
Bso31I GGTCTC 1 cut(s) 76
Bsp143I GATC 5 cut(s) 196, 202, 357, 366, 481
BspACI CCGC 1 cut(s) 439
BspANI GGCC 1 cut(s) 543
BspCNI CTCAG 3 cut(s) 72, 201, 226
BspDI ATCGAT 1 cut(s) 369
BspFNI CGCG 1 cut(s) 246
BspLI GGNNCC 2 cut(s) 69, 228
BspPI GGATC 2 cut(s) 204, 352
BspT107I GGYRCC 1 cut(s) 67
BspTNI GGTCTC 1 cut(s) 76
BsrBI CCGCTC 1 cut(s) 439
BsrI ACTGG 1 cut(s) 162
BssECI CCNNGG 1 cut(s) 538
BssMI GATC 5 cut(s) 196, 202, 357, 366, 481
BssNI GRCGYC 1 cut(s) 597
BssSI CACGAG 1 cut(s) 97
Bst2BI CACGAG 1 cut(s) 97
Bst4CI ACNGT 1 cut(s) 491
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 597
BstDEI CTNAG 3 cut(s) 80, 209, 234
BstF5I GGATG 2 cut(s) 330, 585
BstFNI CGCG 1 cut(s) 246
BstH2I RGCGCY 1 cut(s) 666
BstHHI GCGC 3 cut(s) 246, 282, 665
BstKTI GATC 5 cut(s) 199, 205, 360, 369, 484
BstMAI GTCTC 2 cut(s) 76, 375
BstMBI GATC 5 cut(s) 196, 202, 357, 366, 481
BstMWI GCNNNNNNNGC 2 cut(s) 235, 279
BstUI CGCG 1 cut(s) 246
Bsu15I ATCGAT 1 cut(s) 369
BsuRI GGCC 1 cut(s) 543
BsuTUI ATCGAT 1 cut(s) 369
BtgZI GCGATG 1 cut(s) 487
BtsCI GGATG 2 cut(s) 330, 585
BtsIMutI CAGTG 1 cut(s) 169
CfoI GCGC 3 cut(s) 246, 282, 665
Cfr13I GGNCC 1 cut(s) 522
ClaI ATCGAT 1 cut(s) 369
CseI GACGC 1 cut(s) 379
Csp6I GTAC 2 cut(s) 68, 418
CviAII CATG 2 cut(s) 355, 457
CviQI GTAC 2 cut(s) 68, 418
DdeI CTNAG 3 cut(s) 80, 209, 234
DpnI GATC 5 cut(s) 198, 204, 359, 368, 483
DpnII GATC 5 cut(s) 196, 202, 357, 366, 481
DrdI GACNNNNNNGTC 1 cut(s) 389
DseDI GACNNNNNNGTC 1 cut(s) 389
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
Eco31I GGTCTC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 522
Eco47III AGCGCT 1 cut(s) 664
Eco57I CTGAAG 1 cut(s) 260
EcoT22I ATGCAT 1 cut(s) 52
Esp3I CGTCTC 1 cut(s) 375
FaeI CATG 2 cut(s) 358, 460
FaiI YATR 7 cut(s) 116, 356, 428, 458, 563, 587, 673
FatI CATG 2 cut(s) 354, 456
FblI GTMKAC 4 cut(s) 375, 393, 510, 594
FokI GGATG 2 cut(s) 317, 592
GlaI GCGC 3 cut(s) 245, 281, 664
GsaI CCCAGC 1 cut(s) 302
GsuI CTGGAG 1 cut(s) 332
HaeII RGCGCY 1 cut(s) 666
HaeIII GGCC 1 cut(s) 543
HgaI GACGC 1 cut(s) 379
HhaI GCGC 3 cut(s) 246, 282, 665
Hin1I GRCGYC 1 cut(s) 597
Hin1II CATG 2 cut(s) 358, 460
Hin6I GCGC 3 cut(s) 244, 280, 663
HinP1I GCGC 3 cut(s) 244, 280, 663
HincII GTYRAC 4 cut(s) 376, 394, 511, 595
HindII GTYRAC 4 cut(s) 376, 394, 511, 595
HinfI GANTC 2 cut(s) 213, 305
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 4 cut(s) 376, 394, 511, 595
Hpy188I TCNGA 2 cut(s) 410, 486
Hpy188III TCNNGA 5 cut(s) 19, 110, 194, 200, 361
Hpy8I GTNNAC 4 cut(s) 376, 394, 511, 595
Hpy99I CGWCG 7 cut(s) 278, 380, 395, 512, 515, 599, 602
HpyAV CCTTC 2 cut(s) 454, 640
HpyCH4III ACNGT 1 cut(s) 491
HpyCH4IV ACGT 2 cut(s) 474, 597
HpyCH4V TGCA 2 cut(s) 50, 573
HpyF10VI GCNNNNNNNGC 2 cut(s) 235, 279
HpyF3I CTNAG 3 cut(s) 80, 209, 234
HpySE526I ACGT 2 cut(s) 474, 597
Hsp92I GRCGYC 1 cut(s) 597
Hsp92II CATG 2 cut(s) 358, 460
HspAI GCGC 3 cut(s) 244, 280, 663
KpnI GGTACC 1 cut(s) 71
Kzo9I GATC 5 cut(s) 196, 202, 357, 366, 481
LmnI GCTCC 3 cut(s) 226, 295, 343
LweI GCATC 2 cut(s) 291, 333
MaeII ACGT 2 cut(s) 474, 597
MalI GATC 5 cut(s) 198, 204, 359, 368, 483
MbiI CCGCTC 1 cut(s) 439
MboI GATC 5 cut(s) 196, 202, 357, 366, 481
MboII GAAGA 4 cut(s) 32, 80, 319, 376
MluCI AATT 1 cut(s) 619
Mph1103I ATGCAT 1 cut(s) 52
MseI TTAA 2 cut(s) 45, 668
MslI CAYNNNNRTG 1 cut(s) 221
MspA1I CMGCKG 1 cut(s) 208
Mva1269I GAATGC 2 cut(s) 50, 467
MvnI CGCG 1 cut(s) 246
MwoI GCNNNNNNNGC 2 cut(s) 235, 279
NdeII GATC 5 cut(s) 196, 202, 357, 366, 481
NlaIII CATG 2 cut(s) 358, 460
NlaIV GGNNCC 2 cut(s) 69, 228
NmeAIII GCCGAG 1 cut(s) 519
NsiI ATGCAT 1 cut(s) 52
PcsI WCGNNNNNNNCGW 4 cut(s) 390, 399, 441, 510
PctI GAATGC 2 cut(s) 50, 467
PfeI GAWTC 2 cut(s) 213, 305
Psp1406I AACGTT 1 cut(s) 474
PspFI CCCAGC 1 cut(s) 298
PspN4I GGNNCC 2 cut(s) 69, 228
PspPI GGNCC 1 cut(s) 522
PvuII CAGCTG 1 cut(s) 208
RsaI GTAC 2 cut(s) 69, 419
RsaNI GTAC 2 cut(s) 68, 418
RseI CAYNNNNRTG 1 cut(s) 221
SalI GTCGAC 4 cut(s) 374, 392, 509, 593
SaqAI TTAA 2 cut(s) 45, 668
Sau3AI GATC 5 cut(s) 196, 202, 357, 366, 481
Sau96I GGNCC 1 cut(s) 522
SetI ASST 9 cut(s) 88, 210, 240, 300, 348, 417, 477, 524, 600
SfaNI GCATC 2 cut(s) 291, 333
SgrDI CGTCGACG 1 cut(s) 509
SinI GGWCC 1 cut(s) 522
SmiMI CAYNNNNRTG 1 cut(s) 221
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
Sse9I AATT 1 cut(s) 619
SsiI CCGC 1 cut(s) 439
TaaI ACNGT 1 cut(s) 491
TaiI ACGT 2 cut(s) 477, 600
TaqI TCGA 8 cut(s) 261, 369, 375, 393, 480, 510, 594, 600
TasI AATT 1 cut(s) 619
TfiI GAWTC 2 cut(s) 213, 305
Tru1I TTAA 2 cut(s) 45, 668
Tru9I TTAA 2 cut(s) 45, 668
TscAI CASTG 1 cut(s) 169
TspDTI ATGAA 5 cut(s) 319, 473, 490, 602, 659
TspGWI ACGGA 2 cut(s) 393, 514
TspRI CASTG 1 cut(s) 169
VpaK11BI GGWCC 1 cut(s) 522
XmiI GTMKAC 4 cut(s) 375, 393, 510, 594
ZraI GACGTC 1 cut(s) 598
Zsp2I ATGCAT 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.